Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008194

Physical Location & Seq

Genomic Coordinates Reverse (-)

5274478 .. 5277841

Gene Structure 3364 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1065900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2220 bp

ATGGCCTCCCATTACTCCTCAGACGACGCTGTTCCACCCATCAATGCAACCGCCGTGGCCGTCGACAAGGACAACAAGAACAGCAACTATGCCGTCCGGTGGGCTATCGATCATCTTGTAATCGCCAGCGCCACTCCGTACATCATCCTCATCCATGTCAAGCACAAAAACCATCAGAGTGATGGTGAGGGTGATAATAATGGACATAGCGTTTTCATTCCTTACCGTGCATATTGCGCTCGTAAAGGGGCACAAGTGAAAGAGGTTATCCTTGATGAAGGTGATGTTGCAAAAGCACTTTTGGAATATATCAACTGCAACTACCTCAATAGTATTGTAGTTGGTGCATCAACAAGGAATGCTCTTACAAGGAAGTTCAAAAGTGGGAATGATGTGTCCAGCAGCCTTGTAAAACTTGCACCGGAATTCTGTTCGGTATACGTCATTCAGAAAGGGAAGATGTTGTCAACAAGACCTGCAAAGAGGCCTGCGCTTAATACTGCAGCTCCACCAAAACAGCCATCCTCTCTGGGACTCCCACCTTATATCCCAGCAGATCATAATGAATTTGAAAACGGAGCTAGAGGACAGCCTGCATCAAGGGGAGGGTGGAAAGCTCCAGGATCAGAGAAAATGCCTGCGGCGCGCGAGAGGACAAGGAGTGCTCCAAACAATCTCTCGTTGGATATTAATCTCGACATGGCTAACCATACTTCAGCACGGCCTTCGCTTAGCTGCCGCACTTCGAATGCTGATGAACGTGACTTGTCAATGCCGTTATTTGGGTCAATGGATATTACAAACCAATGCTTCGATTTCTCTAGTGCATTAAATTCTCCCAAGGAGTCATCCAGACAATCTGCACGGGATTTGGAGGCTGAAATGAGAAGATTAAAGCTCGAATTGAAGCAAACAATGGACATGTACAGCTCGGCGTGCAAAGAAGCAATCTTGGCAAAAAATAAGGCTAAAGAGCTTAGTCAGTGGAAGCAAGAGGAGGCGCGAAAGTTCGATGAAGTGAGGGTTGCTGAAGAGACAGCCCTTGCTATTGTGGAAATGGAGAAGGCTAAGTGCAAAGCAGCTATAGAAGCAGCTGAGGCGGCGCAGAAGTTGGCGGAGATGGAAGCTCAGAGAAGAAGACAGGCAGAGATGAAGGCAAAGAAAGAGGCAGAAGAGAAGATCCGGGCGTTGAATGCTTTGGCACAAAATGATGTCCGGTATAGGAAGTACACCATAGAAGAAATTGAAGAAGCCACGGAAAAATTTGCAGAAGCGAATAAAATTGGTGAGGGTGGATATGGACCTGTGTACAAAGGAAAACTCGATCACACGCCTGTTGCCATCAAGGTTCTGAGACCTGATGCTGCTCAAGGGAGGAAGCAATTCCAACAGGAGGTTGAGGTCCTGAGCTGCCTGAGATATCCTAACATGGTTCTCCTCCTTGGTGCTTGCCCCGAATACGGATGCCTAGTATACGAATACATGGAATACGGCAGCTTAGAAGACCGGTTATTTAGAAGAGGTAACACGCCCCCAATATCGTGGAGGAGACGGTTCAAAATAGCTTCTGAGATTTCAATAACGCTTCATTTCCTTCACCAAGCAAAGCCGGAACCCCTTGTACACCGTGACCTAAAGCCAGCAAACATCTTATTAGACCGGAACTTTGTGAGCAAGATCAGCGATGTCGGGCTAGCACGCTTAGTTCCACCATCTGTAGCAGACGAGGTGACACAATACCACATGACTGCAGCCGCGGGAACATTTTGTTACATCGATCCTGAGTACCAACAAACTGGCATGTTAACAACAAAATCAGATATATACTCGTTGGGGATAGTACTACTCCAAATCATCACAGCCAAGCCTGCCATGGGAGTTGCTCATCATGTCAGGAGGTCCATCGAGAAGGGAACATTTTCAGAGATGCTTGATCCAGCAGTGACCGACTGGCCAATCGAAGAGGCTCTAGCGTTCGCTAACTTGGCATTACAATGCACAGAGCTAAGGAAGAAGGACAGGCCTGATCTTGGCACGGTTGTTGTGCCGGAACTGAACCGGTTAAGAGACTTTGGAAGGACTGGCGACATTACTCCTCCTGGGTTCAATTATAGTCCAACTCGAAGCTCTGCGCCGCGTCCTTCCACATCAAGAACGTCACCTACAAGTAGTCAGGAGCCACTGGATAAGAGTAGCTCCGTGGGTGGAGTTGAAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Usp

Protein Analysis

740

Amino Acids

81.89

Weight (kDa)

8.49

Isoelectric Point (pI)

51.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	423 - 675	2.6e-36	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	426 - 680	3.4e-38	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008194)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12000 AT5G12000 AT5G26150 AT5G26150
fragaria_vesca	FvH4_2g31490 FvH4_2g31490
malus_domestica	MD08G1065900.v1.1 MD15G1055500.v1.1
prunus_persica	Prupe.1G408600_v2.0.a1 Prupe.1G408600_v2.0.a1
pyrus_communis	pycom08g05450
rosa_chinensis	RchiOBHm_Chr6g0311301
rosa_laevigata	RLG00000010420
rosa_multiflora	Rmu_co8488183.1_g000001
rosa_roxburghii	Rroxscaffold_7G00157780
rosa_rugosa	Rorug06G0387100 Rorug06G0387100 Rorug06G0387100
rosa_samantha	Rh6AG501900 Rh6BG512600 Rh6CG518300 Rh6DG504500
rosa_wichuraiana	Rw6G043710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	484
AccI	GTMKAC	3 cut(s)	63, 438, 1473
AccII	CGCG	5 cut(s)	646, 648, 1003, 1757, 2137
AciI	CCGC	8 cut(s)	51, 641, 739, 1100, 1115, 1755, 1757, 2135
AclWI	GGATC	4 cut(s)	631, 1174, 1772, 1928
AcoI	YGGCCR	2 cut(s)	57, 1952
AcsI	RAATTY	4 cut(s)	425, 566, 832, 1262
AcuI	CTGAAG	2 cut(s)	699, 1050
AfaI	GTAC	7 cut(s)	140, 926, 1229, 1310, 1623, 1787, 1842
AfiI	CCNNNNNNNGG	6 cut(s)	99, 782, 1393, 1460, 1874, 2030
AflIII	ACRYGT	1 cut(s)	921
AgeI	ACCGGT	2 cut(s)	1506, 2058
AgsI	TTSAA	9 cut(s)	379, 572, 907, 1192, 1247, 1558, 1578, 2107, 2213
AjnI	CCWGG	2 cut(s)	619, 2098
Alw21I	GWGCWC	1 cut(s)	667
Alw26I	GTCTC	4 cut(s)	1028, 1348, 1543, 2061
AlwI	GGATC	4 cut(s)	631, 1174, 1772, 1928
AoxI	GGCC	6 cut(s)	3, 57, 485, 722, 1952, 2021
ApeKI	GCWGC	9 cut(s)	402, 503, 735, 1079, 1091, 1364, 1410, 1494, 1751
ApoI	RAATTY	4 cut(s)	425, 566, 832, 1262
ArsI	GACNNNNNNTTYG	2 cut(s)	1649, 1681
AseI	ATTAAT	1 cut(s)	690
AsiGI	ACCGGT	2 cut(s)	1506, 2058
Asp700I	GAANNNNTTC	2 cut(s)	1382, 1918
AspLEI	GCGC	8 cut(s)	131, 239, 493, 646, 648, 1003, 1105, 2134
AspS9I	GGNCC	3 cut(s)	1301, 1402, 1899
AsuC2I	CCSGG	1 cut(s)	1184
AsuHPI	GGTGA	7 cut(s)	197, 203, 293, 1298, 1589, 1741, 2151
AsuII	TTCGAA	1 cut(s)	746
AsuNHI	GCTAGC	1 cut(s)	1693
AvaII	GGWCC	3 cut(s)	1301, 1402, 1899
BaeGI	GKGCMC	1 cut(s)	253
BalI	TGGCCA	1 cut(s)	1954
BbsI	GAAGAC	2 cut(s)	1144, 1509
Bbv12I	GWGCWC	1 cut(s)	667
BbvCI	CCTCAGC	1 cut(s)	1095
BbvI	GCAGC	9 cut(s)	414, 515, 722, 1091, 1103, 1351, 1397, 1506, 1763
BccI	CCATC	8 cut(s)	47, 176, 180, 529, 1114, 1349, 1720, 1910
BceAI	ACGGC	6 cut(s)	38, 44, 77, 737, 760, 1507
BcgI	CGANNNNNNTGC	2 cut(s)	708, 742
BciT130I	CCWGG	2 cut(s)	621, 2100
BcnI	CCSGG	1 cut(s)	1184
BcoDI	GTCTC	4 cut(s)	1028, 1348, 1543, 2061
BfaI	CTAG	5 cut(s)	582, 822, 1469, 1694, 1970
BfmI	CTRYAG	4 cut(s)	501, 1083, 1716, 1749
BfoI	RGCGCY	1 cut(s)	132
BfuAI	ACCTGC	1 cut(s)	484
BlpI	GCTNAGC	1 cut(s)	731
BmcAI	AGTACT	1 cut(s)	1842
Bme1390I	CCNGG	3 cut(s)	621, 1184, 2100
Bme18I	GGWCC	3 cut(s)	1301, 1402, 1899
BmgT120I	GGNCC	3 cut(s)	1301, 1402, 1899
BmiI	GGNNCC	2 cut(s)	1614, 2178
BmrFI	CCNGG	3 cut(s)	621, 1184, 2100
BmsI	GCATC	5 cut(s)	356, 605, 1351, 1454, 1917
BmtI	GCTAGC	1 cut(s)	1697
BpiI	GAAGAC	2 cut(s)	1144, 1509
BpmI	CTGGAG	1 cut(s)	603
Bpu10I	CCTNAGC	3 cut(s)	1095, 1406, 2006
Bpu1102I	GCTNAGC	1 cut(s)	731
Bpu14I	TTCGAA	1 cut(s)	746
BpuEI	CTTGAG	1 cut(s)	1353
BpuMI	CCSGG	1 cut(s)	1184
Bsa29I	ATCGAT	2 cut(s)	108, 1776
BsaBI	GATNNNNATC	1 cut(s)	690
BsaI	GGTCTC	1 cut(s)	1348
BsaJI	CCNNGG	8 cut(s)	54, 840, 1254, 1441, 1755, 1872, 2099, 2199
BsaWI	WCCGGW	6 cut(s)	96, 421, 1215, 1506, 1659, 2058
BsaXI	ACNNNNNCTCC	2 cut(s)	490, 520
Bsc4I	CCNNNNNNNGG	6 cut(s)	99, 782, 1393, 1460, 1874, 2030
Bse118I	RCCGGY	2 cut(s)	1506, 2058
Bse1I	ACTGG	4 cut(s)	1801, 1955, 2086, 2187
Bse8I	GATNNNNATC	1 cut(s)	690
BseBI	CCWGG	2 cut(s)	621, 2100
BseCI	ATCGAT	2 cut(s)	108, 1776
BseDI	CCNNGG	8 cut(s)	54, 840, 1254, 1441, 1755, 1872, 2099, 2199
BseGI	GGATG	5 cut(s)	144, 150, 521, 848, 1469
BseJI	GATNNNNATC	1 cut(s)	690
BseLI	CCNNNNNNNGG	6 cut(s)	99, 782, 1393, 1460, 1874, 2030
BseMII	CTCAG	8 cut(s)	33, 1086, 1142, 1343, 1397, 1406, 1560, 1773
BseNI	ACTGG	4 cut(s)	1801, 1955, 2086, 2187
BsePI	GCGCGC	1 cut(s)	644
BseRI	GAGGAG	5 cut(s)	7, 1010, 1427, 1561, 2085
BseSI	GKGCMC	1 cut(s)	253
BseXI	GCAGC	9 cut(s)	414, 515, 722, 1091, 1103, 1351, 1397, 1506, 1763
BseYI	CCCAGC	1 cut(s)	550
BsgI	GTGCAG	1 cut(s)	846
Bsh1236I	CGCG	5 cut(s)	646, 648, 1003, 1757, 2137
BshFI	GGCC	6 cut(s)	5, 59, 487, 724, 1954, 2023
BshTI	ACCGGT	2 cut(s)	1506, 2058
BshVI	ATCGAT	2 cut(s)	108, 1776
BsiHKAI	GWGCWC	1 cut(s)	667
BsiSI	CCGG	9 cut(s)	97, 422, 1183, 1216, 1507, 1610, 1660, 2048, 2059
BslFI	GGGAC	1 cut(s)	546
BslI	CCNNNNNNNGG	6 cut(s)	99, 782, 1393, 1460, 1874, 2030
BsmAI	GTCTC	4 cut(s)	1028, 1348, 1543, 2061
BsmBI	CGTCTC	1 cut(s)	1543
BsmFI	GGGAC	1 cut(s)	546
BsmI	GAATGC	3 cut(s)	364, 754, 1198
BsnI	GGCC	6 cut(s)	5, 59, 487, 724, 1954, 2023
Bso31I	GGTCTC	1 cut(s)	1348
Bsp119I	TTCGAA	1 cut(s)	746
Bsp1286I	GDGCHC	2 cut(s)	253, 667
Bsp1407I	TGTACA	3 cut(s)	924, 1308, 1621
Bsp143I	GATC	9 cut(s)	109, 556, 623, 1179, 1324, 1677, 1777, 1933, 2026
Bsp1720I	GCTNAGC	1 cut(s)	731
Bsp19I	CCATGG	1 cut(s)	1872
BspACI	CCGC	8 cut(s)	51, 641, 739, 1100, 1115, 1755, 1757, 2135
BspANI	GGCC	6 cut(s)	5, 59, 487, 724, 1954, 2023
BspCNI	CTCAG	8 cut(s)	32, 1087, 1141, 1344, 1398, 1407, 1561, 1774
BspDI	ATCGAT	2 cut(s)	108, 1776
BspFNI	CGCG	5 cut(s)	646, 648, 1003, 1757, 2137
BspLI	GGNNCC	2 cut(s)	1614, 2178
BspMAI	CTGCAG	2 cut(s)	505, 1753
BspMI	ACCTGC	1 cut(s)	484
BspOI	GCTAGC	1 cut(s)	1697
BspPI	GGATC	4 cut(s)	631, 1174, 1772, 1928
BspT104I	TTCGAA	1 cut(s)	746
BspTNI	GGTCTC	1 cut(s)	1348
BsrFI	RCCGGY	2 cut(s)	1506, 2058
BsrGI	TGTACA	3 cut(s)	924, 1308, 1621
BsrI	ACTGG	4 cut(s)	1801, 1955, 2086, 2187
BssAI	RCCGGY	2 cut(s)	1506, 2058
BssECI	CCNNGG	8 cut(s)	54, 840, 1254, 1441, 1755, 1872, 2099, 2199
BssHII	GCGCGC	1 cut(s)	644
BssMI	GATC	9 cut(s)	109, 556, 623, 1179, 1324, 1677, 1777, 1933, 2026
BssNAI	GTATAC	2 cut(s)	439, 1474
BssT1I	CCWWGG	3 cut(s)	840, 1441, 1872
Bst1107I	GTATAC	2 cut(s)	439, 1474
Bst2UI	CCWGG	2 cut(s)	621, 2100
Bst4CI	ACNGT	4 cut(s)	227, 1554, 1628, 2038
Bst6I	CTCTTC	4 cut(s)	1026, 1167, 1513, 1956
BstAUI	TGTACA	3 cut(s)	924, 1308, 1621
BstBI	TTCGAA	1 cut(s)	746
BstDSI	CCRYGG	5 cut(s)	54, 1254, 1755, 1872, 2199
BstF5I	GGATG	5 cut(s)	144, 150, 521, 848, 1469
BstFNI	CGCG	5 cut(s)	646, 648, 1003, 1757, 2137
BstH2I	RGCGCY	1 cut(s)	132
BstHHI	GCGC	8 cut(s)	131, 239, 493, 646, 648, 1003, 1105, 2134
BstKTI	GATC	9 cut(s)	112, 559, 626, 1182, 1327, 1680, 1780, 1936, 2029
BstMAI	GTCTC	4 cut(s)	1028, 1348, 1543, 2061
BstMBI	GATC	9 cut(s)	109, 556, 623, 1179, 1324, 1677, 1777, 1933, 2026
BstNI	CCWGG	2 cut(s)	621, 2100
BstNSI	RCATGY	2 cut(s)	925, 1804
BstSCI	CCNGG	3 cut(s)	619, 1182, 2098
BstSFI	CTRYAG	4 cut(s)	501, 1083, 1716, 1749
BstSLI	GKGCMC	1 cut(s)	253
BstUI	CGCG	5 cut(s)	646, 648, 1003, 1757, 2137
BstV1I	GCAGC	9 cut(s)	414, 515, 722, 1091, 1103, 1351, 1397, 1506, 1763
BstV2I	GAAGAC	2 cut(s)	1144, 1509
BstX2I	RGATCY	1 cut(s)	1179
BstXI	CCANNNNNNTGG	2 cut(s)	1542, 1796
BstYI	RGATCY	1 cut(s)	1179
BstZ17I	GTATAC	2 cut(s)	439, 1474
Bsu15I	ATCGAT	2 cut(s)	108, 1776
BsuRI	GGCC	6 cut(s)	5, 59, 487, 724, 1954, 2023
BsuTUI	ATCGAT	2 cut(s)	108, 1776
BtgI	CCRYGG	5 cut(s)	54, 1254, 1755, 1872, 2199
BtgZI	GCGATG	1 cut(s)	1698
BtsCI	GGATG	5 cut(s)	144, 150, 521, 848, 1469
BtsI	GCAGTG	1 cut(s)	1947
BtsIMutI	CAGTG	3 cut(s)	989, 1947, 2180
BveI	ACCTGC	1 cut(s)	484
CfoI	GCGC	8 cut(s)	131, 239, 493, 646, 648, 1003, 1105, 2134
Cfr10I	RCCGGY	2 cut(s)	1506, 2058
Cfr13I	GGNCC	3 cut(s)	1301, 1402, 1899
Cfr42I	CCGCGG	1 cut(s)	1758
ClaI	ATCGAT	2 cut(s)	108, 1776
CseI	GACGC	2 cut(s)	35, 2126
Csp6I	GTAC	7 cut(s)	139, 925, 1228, 1309, 1622, 1786, 1841
CspAI	ACCGGT	2 cut(s)	1506, 2058
CspCI	CAANNNNNGTGG	2 cut(s)	36, 71
CviAII	CATG	9 cut(s)	155, 700, 922, 1429, 1483, 1744, 1801, 1873, 1889
CviQI	GTAC	7 cut(s)	139, 925, 1228, 1309, 1622, 1786, 1841
DpnI	GATC	9 cut(s)	111, 558, 625, 1181, 1326, 1679, 1779, 1935, 2028
DpnII	GATC	9 cut(s)	109, 556, 623, 1179, 1324, 1677, 1777, 1933, 2026
EaeI	YGGCCR	2 cut(s)	57, 1952
Eam1104I	CTCTTC	4 cut(s)	1026, 1167, 1513, 1956
EarI	CTCTTC	4 cut(s)	1026, 1167, 1513, 1956
EciI	GGCGGA	1 cut(s)	1130
Eco130I	CCWWGG	3 cut(s)	840, 1441, 1872
Eco147I	AGGCCT	2 cut(s)	487, 2023
Eco31I	GGTCTC	1 cut(s)	1348
Eco32I	GATATC	1 cut(s)	1421
Eco47I	GGWCC	3 cut(s)	1301, 1402, 1899
Eco57I	CTGAAG	2 cut(s)	699, 1050
EcoO109I	RGGNCCY	1 cut(s)	1402
EcoRI	GAATTC	1 cut(s)	425
EcoRII	CCWGG	2 cut(s)	619, 2098
EcoRV	GATATC	1 cut(s)	1421
EcoT14I	CCWWGG	3 cut(s)	840, 1441, 1872
ErhI	CCWWGG	3 cut(s)	840, 1441, 1872
Esp3I	CGTCTC	1 cut(s)	1543
FaeI	CATG	9 cut(s)	158, 703, 925, 1432, 1486, 1747, 1804, 1876, 1892
FaqI	GGGAC	1 cut(s)	546
FatI	CATG	9 cut(s)	154, 699, 921, 1428, 1482, 1743, 1800, 1872, 1888
FauI	CCCGC	1 cut(s)	1750
FblI	GTMKAC	3 cut(s)	63, 438, 1473
FokI	GGATG	5 cut(s)	131, 137, 508, 835, 1476
FspBI	CTAG	5 cut(s)	582, 822, 1469, 1694, 1970
GlaI	GCGC	8 cut(s)	130, 238, 492, 645, 647, 1002, 1104, 2133
GsaI	CCCAGC	1 cut(s)	554
GsuI	CTGGAG	1 cut(s)	603
HaeII	RGCGCY	1 cut(s)	132
HaeIII	GGCC	6 cut(s)	5, 59, 487, 724, 1954, 2023
HapII	CCGG	9 cut(s)	97, 422, 1183, 1216, 1507, 1610, 1660, 2048, 2059
HgaI	GACGC	2 cut(s)	35, 2126
HhaI	GCGC	8 cut(s)	131, 239, 493, 646, 648, 1003, 1105, 2134
Hin1II	CATG	9 cut(s)	158, 703, 925, 1432, 1486, 1747, 1804, 1876, 1892
Hin6I	GCGC	8 cut(s)	129, 237, 491, 644, 646, 1001, 1103, 2132
HinP1I	GCGC	8 cut(s)	129, 237, 491, 644, 646, 1001, 1103, 2132
HincII	GTYRAC	3 cut(s)	64, 468, 1806
HindII	GTYRAC	3 cut(s)	64, 468, 1806
HinfI	GANTC	2 cut(s)	534, 845
HpaI	GTTAAC	1 cut(s)	1806
HpaII	CCGG	9 cut(s)	97, 422, 1183, 1216, 1507, 1610, 1660, 2048, 2059
HphI	GGTGA	7 cut(s)	197, 203, 293, 1298, 1589, 1741, 2151
Hpy166II	GTNNAC	8 cut(s)	64, 439, 468, 1230, 1309, 1474, 1624, 1806
Hpy188I	TCNGA	9 cut(s)	22, 177, 450, 628, 1131, 1353, 1570, 1819, 1924
Hpy188III	TCNNGA	8 cut(s)	695, 852, 1405, 1781, 1894, 1906, 2151, 2174
Hpy8I	GTNNAC	8 cut(s)	64, 439, 468, 1230, 1309, 1474, 1624, 1806
Hpy99I	CGWCG	2 cut(s)	29, 65
HpyAV	CCTTC	9 cut(s)	272, 735, 1057, 1147, 1604, 1903, 2008, 2070, 2151
HpyCH4III	ACNGT	4 cut(s)	227, 1554, 1628, 2038
HpyCH4IV	ACGT	3 cut(s)	441, 760, 2156
HpySE526I	ACGT	3 cut(s)	441, 760, 2156
Hsp92II	CATG	9 cut(s)	158, 703, 925, 1432, 1486, 1747, 1804, 1876, 1892
HspAI	GCGC	8 cut(s)	129, 237, 491, 644, 646, 1001, 1103, 2132
KspAI	GTTAAC	1 cut(s)	1806
KspI	CCGCGG	1 cut(s)	1758
Kzo9I	GATC	9 cut(s)	109, 556, 623, 1179, 1324, 1677, 1777, 1933, 2026
LmnI	GCTCC	6 cut(s)	511, 578, 622, 670, 2176, 2201
Lsp1109I	GCAGC	9 cut(s)	414, 515, 722, 1091, 1103, 1351, 1397, 1506, 1763
LweI	GCATC	5 cut(s)	356, 605, 1351, 1454, 1917
MaeI	CTAG	5 cut(s)	582, 822, 1469, 1694, 1970
MaeII	ACGT	3 cut(s)	441, 760, 2156
MaeIII	GTNAC	7 cut(s)	761, 1523, 1628, 1729, 1769, 1942, 2157
MalI	GATC	9 cut(s)	111, 558, 625, 1181, 1326, 1679, 1779, 1935, 2028
MboI	GATC	9 cut(s)	109, 556, 623, 1179, 1324, 1677, 1777, 1933, 2026
MflI	RGATCY	1 cut(s)	1179
MhlI	GDGCHC	2 cut(s)	253, 667
MlsI	TGGCCA	1 cut(s)	1954
MluCI	AATT	9 cut(s)	425, 566, 832, 902, 1242, 1262, 1281, 1382, 2107
MluNI	TGGCCA	1 cut(s)	1954
MlyI	GAGTC	2 cut(s)	528, 854
MmeI	TCCRAC	3 cut(s)	663, 1411, 2141
Mox20I	TGGCCA	1 cut(s)	1954
MroXI	GAANNNNTTC	2 cut(s)	1382, 1918
MscI	TGGCCA	1 cut(s)	1954
MseI	TTAA	6 cut(s)	495, 690, 830, 893, 1805, 2063
MslI	CAYNNNNRTG	2 cut(s)	177, 1993
Msp20I	TGGCCA	1 cut(s)	1954
MspA1I	CMGCKG	2 cut(s)	1094, 1757
MspI	CCGG	9 cut(s)	97, 422, 1183, 1216, 1507, 1610, 1660, 2048, 2059
MspR9I	CCNGG	3 cut(s)	621, 1184, 2100
Mva1269I	GAATGC	3 cut(s)	364, 754, 1198
MvaI	CCWGG	2 cut(s)	621, 2100
MvnI	CGCG	5 cut(s)	646, 648, 1003, 1757, 2137
NciI	CCSGG	1 cut(s)	1184
NcoI	CCATGG	1 cut(s)	1872
NdeII	GATC	9 cut(s)	109, 556, 623, 1179, 1324, 1677, 1777, 1933, 2026
NheI	GCTAGC	1 cut(s)	1693
NlaIII	CATG	9 cut(s)	158, 703, 925, 1432, 1486, 1747, 1804, 1876, 1892
NlaIV	GGNNCC	2 cut(s)	1614, 2178
NmeAIII	GCCGAG	1 cut(s)	911
NmuCI	GTSAC	5 cut(s)	761, 1628, 1729, 1942, 2157
NspI	RCATGY	2 cut(s)	925, 1804
NspV	TTCGAA	1 cut(s)	746
PauI	GCGCGC	1 cut(s)	644
PceI	AGGCCT	2 cut(s)	487, 2023
PciI	ACATGT	1 cut(s)	921
PctI	GAATGC	3 cut(s)	364, 754, 1198
PdmI	GAANNNNTTC	2 cut(s)	1382, 1918
PfoI	TCCNGGA	1 cut(s)	619
PinAI	ACCGGT	2 cut(s)	1506, 2058
PleI	GAGTC	2 cut(s)	528, 853
PpsI	GAGTC	2 cut(s)	528, 853
PpuMI	RGGWCCY	1 cut(s)	1402
PscI	ACATGT	1 cut(s)	921
PshBI	ATTAAT	1 cut(s)	690
Psp5II	RGGWCCY	1 cut(s)	1402
Psp6I	CCWGG	2 cut(s)	619, 2098
PspFI	CCCAGC	1 cut(s)	550
PspGI	CCWGG	2 cut(s)	619, 2098
PspN4I	GGNNCC	2 cut(s)	1614, 2178
PspPI	GGNCC	3 cut(s)	1301, 1402, 1899
PspPPI	RGGWCCY	1 cut(s)	1402
PsrI	GAACNNNNNNTAC	4 cut(s)	1605, 1637, 2146, 2178
PstI	CTGCAG	2 cut(s)	505, 1753
PsuI	RGATCY	1 cut(s)	1179
PteI	GCGCGC	1 cut(s)	644
PvuII	CAGCTG	1 cut(s)	1094
RsaI	GTAC	7 cut(s)	140, 926, 1229, 1310, 1623, 1787, 1842
RsaNI	GTAC	7 cut(s)	139, 925, 1228, 1309, 1622, 1786, 1841
RseI	CAYNNNNRTG	2 cut(s)	177, 1993
SacII	CCGCGG	1 cut(s)	1758
SalI	GTCGAC	1 cut(s)	62
SaqAI	TTAA	6 cut(s)	495, 690, 830, 893, 1805, 2063
Sau3AI	GATC	9 cut(s)	109, 556, 623, 1179, 1324, 1677, 1777, 1933, 2026
Sau96I	GGNCC	3 cut(s)	1301, 1402, 1899
ScaI	AGTACT	1 cut(s)	1842
SchI	GAGTC	2 cut(s)	528, 854
ScrFI	CCNGG	3 cut(s)	621, 1184, 2100
SduI	GDGCHC	2 cut(s)	253, 667
SfaNI	GCATC	5 cut(s)	356, 605, 1351, 1454, 1917
SfcI	CTRYAG	4 cut(s)	501, 1083, 1716, 1749
Sfr303I	CCGCGG	1 cut(s)	1758
SfuI	TTCGAA	1 cut(s)	746
SgrBI	CCGCGG	1 cut(s)	1758
SinI	GGWCC	3 cut(s)	1301, 1402, 1899
SmiMI	CAYNNNNRTG	2 cut(s)	177, 1993
SmlI	CTYRAG	1 cut(s)	1368
SmoI	CTYRAG	1 cut(s)	1368
Sse9I	AATT	9 cut(s)	425, 566, 832, 902, 1242, 1262, 1281, 1382, 2107
SseBI	AGGCCT	2 cut(s)	487, 2023
SsiI	CCGC	8 cut(s)	51, 641, 739, 1100, 1115, 1755, 1757, 2135
SspMI	CTAG	5 cut(s)	582, 822, 1469, 1694, 1970
StuI	AGGCCT	2 cut(s)	487, 2023
StyD4I	CCNGG	3 cut(s)	619, 1182, 2098
StyI	CCWWGG	3 cut(s)	840, 1441, 1872
TaaI	ACNGT	4 cut(s)	227, 1554, 1628, 2038
TaiI	ACGT	3 cut(s)	444, 763, 2159
TaqII	GACCGA	1 cut(s)	1961
TasI	AATT	9 cut(s)	425, 566, 832, 902, 1242, 1262, 1281, 1382, 2107
TatI	WGTACW	5 cut(s)	924, 1227, 1308, 1621, 1840
TauI	GCSGC	5 cut(s)	644, 741, 1103, 1757, 2137
Tru1I	TTAA	6 cut(s)	495, 690, 830, 893, 1805, 2063
Tru9I	TTAA	6 cut(s)	495, 690, 830, 893, 1805, 2063
TscAI	CASTG	3 cut(s)	989, 1947, 2187
TseFI	GTSAC	5 cut(s)	761, 1628, 1729, 1942, 2157
TseI	GCWGC	9 cut(s)	402, 503, 735, 1079, 1091, 1364, 1410, 1494, 1751
Tsp45I	GTSAC	5 cut(s)	761, 1628, 1729, 1942, 2157
TspDTI	ATGAA	7 cut(s)	205, 291, 579, 771, 1029, 1166, 1577
TspGWI	ACGGA	5 cut(s)	126, 591, 1271, 1476, 2188
TspRI	CASTG	3 cut(s)	989, 1947, 2187
VpaK11BI	GGWCC	3 cut(s)	1301, 1402, 1899
VspI	ATTAAT	1 cut(s)	690
XapI	RAATTY	4 cut(s)	425, 566, 832, 1262
XceI	RCATGY	2 cut(s)	925, 1804
XcmI	CCANNNNNNNNNTGG	2 cut(s)	179, 1870
XmiI	GTMKAC	3 cut(s)	63, 438, 1473
XmnI	GAANNNNTTC	2 cut(s)	1382, 1918
XspI	CTAG	5 cut(s)	582, 822, 1469, 1694, 1970
ZrmI	AGTACT	1 cut(s)	1842

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.