Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008194

Physical Location & Seq

Genomic Coordinates Forward (+)

3748519 .. 3752095

Gene Structure 3577 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1055500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2220 bp

ATGGCCTCCCATTACTCCTCGGACGACGCCGTTCCACCCATCAATGCAACCGCCGTGGCTGTCGACAAGGACAATAAGAACAGCAACTATGCCGTCCGGTGGGCTATTGATCATCTTGTAACCGCCGGCACCAAGCCGTACATCATCCTCATCCATGTCAAGCACAAAAACAATCGTAGTGATGCTGATTCGGGTGATAATAATGGACGTGGTGTTTTTGTTCCTTACCGTGCATATTGCGCTCGTAAAGGGGCACGAGTGAAAGAGGTTATCCTTGATGACAGTGATGTTGCAAAAGCACTTTTGGAATATGTCAATTGCAACTACCTCAATATTATTGTAGTTGGTGCATCAACAAGGAATGCTCTTACAAGGAAGTTTAAAAGTGGGAATGATGTGCCCAGCAGCATTGTAAAATTAGCGCCGGAATTCTGTTCTGTATACGTAATTCAGAAAGGGAAGATGTTGTCAACAAGAACTGCAAAGAGGCCAGTGGTTAATACTGCAGTTCCACCAAAACAGCCATCCTCCCAGGGACTTCCATATCATATCCCAGCAGATAATAATGAATTTGAAAACGGAGTTAGAGGACAGCCTGCATTAAGGGGAGGGTGGAAGGTTCCAGGACCGGACAAAATGCCTGCAGCGCGCGAGAGGACAAGGAGTGCTCCGAACAATCTCTTGTTGGATATTAATGTCGGCATGGCTAACCATACTGCAGCACGGTCTTCGCTTAGCAACCGCACTTCGAATGCTGATGAAAGTGACTTTTCAATGCCATTGTTTGGATCAGTGGATATGACAAACCAATGTTTCGACTTCTCTAGTGCATTAAATTCTCCCAAGGACTCATCCAGACATTCTGCACGGGATATGGAGGCTGAAATGCGAAGATTAAAGCTCGAATTGAAGCAAACGATGGACATGTACAGCTCGGCCTGCAAAGAAGCAATCTCGGCAAAAAATAAGGCTAACGAGCTTAGTCAGTGGATGCAGGAGGCGCGAAAGTTCGAGGAAGTGAAGCTTGCTGAACAGACGGCCCTTGCCGTTGTGGAAATGGAGAAGGCTAAGTGCAAAGCAGCTATAGAAGCAGCTGAGGCGTCGCAGAAGTTGGCGGAGATGGAAGCTCAGAGAAGAAGACAGGCAGAGATGAAGGCAAAGAAAGAGGCAGAAGAGAAGAACCGGGCGTTGAATGCTTTGGCACATAATGATCTCCGGTATAGGAAGTACACCATTGAAGAAATTGAAGAAGCTACGGAAAATTTTGAATCAGCGAATAAAATTGGTGAGGGTGGATATGGACCTGTGTACAAAGGCAAACTCGATCACACGCCGGTTGCTATCAAAGTTTTGAGACCTGATGCTTCTCAAGGGAGGAAGCAATTCCAACAGGAGGTTGAGGTCCTGAGCTGCATTAGATATCCTAACATGGTTCTCCTCCTTGGTGCCTGCCCCGAATTCGGATGCCTAGTATACGAATACATGGAATACGGAAGCTTAGAAGATCGGTTATTTAGAAGAGGTAACACCCCCCCACTTTCGTGGAGGAGACGGTTCAAAATAGCTGCTGAAATTTCAACAACACTTCATTTCCTTCACCAAGCAAAGCCGGAACCCCTTGTACACCGTGACCTAAAGCCAGCAAACATCCTATTAGACCGGAACTTTGTAAGCAAGATCGGTGATGTCGGGCTAGCACGCTTAGTTCCAGCATCTGTAGCAGACGAGGTGACACAATACCACTTGACTGCAGCCGCCGGAACATTTTGTTACATCGATCCTGAGTACCAACAAACTGGCATGTTAACAACAAAATCGGATATATACTCGTTGGGGATAGTACTACTCCAAATCATCACAGCCAAGCCTGCCATGGGACTTGCTCACCATGTTAGGAGGTCCATCGAGAAGGGAACATTTTCGGAGATGCTTGATCCAGCAGTGACCGACTGGCCAATTGAAGAGGCTCTAGCGTTCGCTAACTTGGCATTACAATGCACAGAGCTAAGGAAGAAGGACAGGCCCGACCTTGGCACAGTTGTAGTGCCAGAACTTATCCGGTTAAGAGACTTTGGAAGGACTGGTGACACTACTCCTCCCGGGTTCAGTGATAGTCCAACTCCAAGCTCTGTGTCGCGTCCTTCCACATCAAGAACATCAACTACAAGTAGTCAGGAGCCACTGAATAAGAGTAGCTCCAGGGTCGGAGTTGAAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Usp

Protein Analysis

740

Amino Acids

81.84

Weight (kDa)

8.77

Isoelectric Point (pI)

50.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	422 - 674	1.7e-35	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	425 - 680	4.6e-38	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008194)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12000 AT5G12000 AT5G26150 AT5G26150
fragaria_vesca	FvH4_2g31490 FvH4_2g31490
malus_domestica	MD08G1065900.v1.1 MD15G1055500.v1.1
prunus_persica	Prupe.1G408600_v2.0.a1 Prupe.1G408600_v2.0.a1
pyrus_communis	pycom08g05450
rosa_chinensis	RchiOBHm_Chr6g0311301
rosa_laevigata	RLG00000010420
rosa_multiflora	Rmu_co8488183.1_g000001
rosa_roxburghii	Rroxscaffold_7G00157780
rosa_rugosa	Rorug06G0387100 Rorug06G0387100 Rorug06G0387100
rosa_samantha	Rh6AG501900 Rh6BG512600 Rh6CG518300 Rh6DG504500
rosa_wichuraiana	Rw6G043710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	128, 1445
AccB7I	CCANNNNNTGG	1 cut(s)	785
AccI	GTMKAC	3 cut(s)	63, 441, 1473
AccII	CGCG	4 cut(s)	649, 651, 1003, 2137
AciI	CCGC	5 cut(s)	51, 123, 742, 1115, 1755
AclWI	GGATC	3 cut(s)	796, 1772, 1928
AcoI	YGGCCR	1 cut(s)	1952
AcsI	RAATTY	6 cut(s)	428, 569, 835, 1261, 1457, 1572
AcyI	GRCGYC	2 cut(s)	27, 1100
AfaI	GTAC	7 cut(s)	140, 929, 1229, 1310, 1623, 1787, 1842
AfiI	CCNNNNNNNGG	6 cut(s)	99, 785, 1393, 1460, 1874, 2030
AflIII	ACRYGT	1 cut(s)	924
AjiI	CACGTC	1 cut(s)	209
AjnI	CCWGG	3 cut(s)	531, 622, 2198
AleI	CACNNNNGTG	1 cut(s)	1540
Alw21I	GWGCWC	1 cut(s)	670
Alw26I	GTCTC	3 cut(s)	1348, 1543, 2061
AlwI	GGATC	3 cut(s)	796, 1772, 1928
AlwNI	CAGNNNCTG	1 cut(s)	1715
Ama87I	CYCGRG	1 cut(s)	2099
AoxI	GGCC	6 cut(s)	3, 488, 936, 1038, 1952, 2021
ApeKI	GCWGC	8 cut(s)	405, 644, 719, 1079, 1091, 1410, 1565, 1751
ApoI	RAATTY	6 cut(s)	428, 569, 835, 1261, 1457, 1572
ArsI	GACNNNNNNTTYG	2 cut(s)	1649, 1681
AseI	ATTAAT	1 cut(s)	693
Asp700I	GAANNNNTTC	2 cut(s)	1382, 1918
AspLEI	GCGC	5 cut(s)	242, 424, 649, 651, 1003
AspS9I	GGNCC	6 cut(s)	626, 1039, 1301, 1402, 1899, 2022
AsuC2I	CCSGG	3 cut(s)	1184, 2100, 2101
AsuHPI	GGTGA	7 cut(s)	206, 1298, 1589, 1694, 1741, 1877, 2096
AsuII	TTCGAA	1 cut(s)	749
AsuNHI	GCTAGC	1 cut(s)	1693
AvaI	CYCGRG	1 cut(s)	2099
AvaII	GGWCC	4 cut(s)	626, 1301, 1402, 1899
BaeGI	GKGCMC	2 cut(s)	256, 402
BalI	TGGCCA	1 cut(s)	1954
BanI	GGYRCC	2 cut(s)	128, 1445
BauI	CACGAG	1 cut(s)	255
BbsI	GAAGAC	2 cut(s)	720, 1144
Bbv12I	GWGCWC	1 cut(s)	670
BbvCI	CCTCAGC	1 cut(s)	1095
BbvI	GCAGC	8 cut(s)	417, 656, 731, 1091, 1103, 1397, 1552, 1763
BccI	CCATC	5 cut(s)	47, 532, 913, 1114, 1910
BceAI	ACGGC	6 cut(s)	14, 38, 77, 121, 1031, 1053
BcgI	CGANNNNNNTGC	2 cut(s)	711, 745
BciT130I	CCWGG	3 cut(s)	533, 624, 2200
BclI	TGATCA	1 cut(s)	109
BcnI	CCSGG	3 cut(s)	1184, 2100, 2101
BcoDI	GTCTC	3 cut(s)	1348, 1543, 2061
BfaI	CTAG	4 cut(s)	825, 1469, 1694, 1970
BfmI	CTRYAG	6 cut(s)	504, 642, 717, 1083, 1716, 1749
BfoI	RGCGCY	1 cut(s)	425
BisI	GCNGC	9 cut(s)	406, 645, 720, 1080, 1092, 1411, 1566, 1752, 1755
BlpI	GCTNAGC	1 cut(s)	734
BlsI	GCNGC	9 cut(s)	407, 646, 721, 1081, 1093, 1412, 1567, 1753, 1756
BmcAI	AGTACT	1 cut(s)	1842
Bme1390I	CCNGG	6 cut(s)	533, 624, 1184, 2100, 2101, 2200
Bme18I	GGWCC	4 cut(s)	626, 1301, 1402, 1899
BmeT110I	CYCGRG	1 cut(s)	2099
BmgBI	CACGTC	1 cut(s)	209
BmgT120I	GGNCC	6 cut(s)	626, 1039, 1301, 1402, 1899, 2022
BmiI	GGNNCC	5 cut(s)	130, 621, 1447, 1614, 2178
BmrFI	CCNGG	6 cut(s)	533, 624, 1184, 2100, 2101, 2200
BmsI	GCATC	7 cut(s)	172, 359, 981, 1351, 1454, 1721, 1917
BmtI	GCTAGC	1 cut(s)	1697
BpiI	GAAGAC	2 cut(s)	720, 1144
BpmI	CTGGAG	1 cut(s)	2182
Bpu10I	CCTNAGC	3 cut(s)	1095, 1406, 2006
Bpu1102I	GCTNAGC	1 cut(s)	734
Bpu14I	TTCGAA	1 cut(s)	749
BpuEI	CTTGAG	1 cut(s)	1353
BpuMI	CCSGG	3 cut(s)	1184, 2100, 2101
Bsa29I	ATCGAT	1 cut(s)	1776
BsaAI	YACGTR	1 cut(s)	445
BsaHI	GRCGYC	2 cut(s)	27, 1100
BsaI	GGTCTC	1 cut(s)	1348
BsaWI	WCCGGW	5 cut(s)	96, 628, 1215, 1659, 2058
BsaXI	ACNNNNNCTCC	2 cut(s)	2080, 2110
Bsc4I	CCNNNNNNNGG	6 cut(s)	99, 785, 1393, 1460, 1874, 2030
Bse118I	RCCGGY	2 cut(s)	125, 1333
Bse1I	ACTGG	4 cut(s)	491, 1801, 1955, 2086
BseBI	CCWGG	3 cut(s)	533, 624, 2200
BseCI	ATCGAT	1 cut(s)	1776
BseGI	GGATG	7 cut(s)	144, 150, 524, 851, 996, 1469, 1647
BseLI	CCNNNNNNNGG	6 cut(s)	99, 785, 1393, 1460, 1874, 2030
BseMII	CTCAG	4 cut(s)	1086, 1142, 1397, 1773
BseNI	ACTGG	4 cut(s)	491, 1801, 1955, 2086
BsePI	GCGCGC	1 cut(s)	647
BseRI	GAGGAG	4 cut(s)	7, 1427, 1561, 2085
BseSI	GKGCMC	2 cut(s)	256, 402
BseXI	GCAGC	8 cut(s)	417, 656, 731, 1091, 1103, 1397, 1552, 1763
BseYI	CCCAGC	2 cut(s)	401, 553
BsgI	GTGCAG	1 cut(s)	849
Bsh1236I	CGCG	4 cut(s)	649, 651, 1003, 2137
BshFI	GGCC	6 cut(s)	5, 490, 938, 1040, 1954, 2023
BshNI	GGYRCC	2 cut(s)	128, 1445
BshVI	ATCGAT	1 cut(s)	1776
BsiHKAI	GWGCWC	1 cut(s)	670
BsiHKCI	CYCGRG	1 cut(s)	2099
BslFI	GGGAC	2 cut(s)	549, 1890
BslI	CCNNNNNNNGG	6 cut(s)	99, 785, 1393, 1460, 1874, 2030
BsmAI	GTCTC	3 cut(s)	1348, 1543, 2061
BsmBI	CGTCTC	1 cut(s)	1543
BsmFI	GGGAC	2 cut(s)	549, 1890
BsmI	GAATGC	3 cut(s)	367, 757, 1198
BsnI	GGCC	6 cut(s)	5, 490, 938, 1040, 1954, 2023
Bso31I	GGTCTC	1 cut(s)	1348
BsoBI	CYCGRG	1 cut(s)	2099
Bsp119I	TTCGAA	1 cut(s)	749
Bsp1286I	GDGCHC	3 cut(s)	256, 402, 670
Bsp1407I	TGTACA	3 cut(s)	927, 1308, 1621
Bsp143I	GATC	8 cut(s)	109, 788, 1210, 1324, 1504, 1677, 1777, 1933
Bsp1720I	GCTNAGC	1 cut(s)	734
Bsp19I	CCATGG	1 cut(s)	1872
BspACI	CCGC	5 cut(s)	51, 123, 742, 1115, 1755
BspANI	GGCC	6 cut(s)	5, 490, 938, 1040, 1954, 2023
BspCNI	CTCAG	4 cut(s)	1087, 1141, 1398, 1774
BspDI	ATCGAT	1 cut(s)	1776
BspFNI	CGCG	4 cut(s)	649, 651, 1003, 2137
BspLI	GGNNCC	5 cut(s)	130, 621, 1447, 1614, 2178
BspMAI	CTGCAG	4 cut(s)	508, 646, 721, 1753
BspOI	GCTAGC	1 cut(s)	1697
BspPI	GGATC	3 cut(s)	796, 1772, 1928
BspT104I	TTCGAA	1 cut(s)	749
BspT107I	GGYRCC	2 cut(s)	128, 1445
BspTNI	GGTCTC	1 cut(s)	1348
BsrFI	RCCGGY	2 cut(s)	125, 1333
BsrGI	TGTACA	3 cut(s)	927, 1308, 1621
BsrI	ACTGG	4 cut(s)	491, 1801, 1955, 2086
BssAI	RCCGGY	2 cut(s)	125, 1333
BssHII	GCGCGC	1 cut(s)	647
BssMI	GATC	8 cut(s)	109, 788, 1210, 1324, 1504, 1677, 1777, 1933
BssNAI	GTATAC	2 cut(s)	442, 1474
BssNI	GRCGYC	2 cut(s)	27, 1100
BssSI	CACGAG	1 cut(s)	255
BssT1I	CCWWGG	4 cut(s)	843, 1441, 1872, 2029
Bst1107I	GTATAC	2 cut(s)	442, 1474
Bst2BI	CACGAG	1 cut(s)	255
Bst2UI	CCWGG	3 cut(s)	533, 624, 2200
Bst4CI	ACNGT	6 cut(s)	230, 284, 726, 1554, 1628, 2038
Bst6I	CTCTTC	3 cut(s)	1167, 1513, 1956
BstACI	GRCGYC	2 cut(s)	27, 1100
BstAUI	TGTACA	3 cut(s)	927, 1308, 1621
BstBAI	YACGTR	1 cut(s)	445
BstBI	TTCGAA	1 cut(s)	749
BstDSI	CCRYGG	2 cut(s)	54, 1872
BstF5I	GGATG	7 cut(s)	144, 150, 524, 851, 996, 1469, 1647
BstFNI	CGCG	4 cut(s)	649, 651, 1003, 2137
BstH2I	RGCGCY	1 cut(s)	425
BstHHI	GCGC	5 cut(s)	242, 424, 649, 651, 1003
BstKTI	GATC	8 cut(s)	112, 791, 1213, 1327, 1507, 1680, 1780, 1936
BstMAI	GTCTC	3 cut(s)	1348, 1543, 2061
BstMBI	GATC	8 cut(s)	109, 788, 1210, 1324, 1504, 1677, 1777, 1933
BstNI	CCWGG	3 cut(s)	533, 624, 2200
BstNSI	RCATGY	2 cut(s)	928, 1804
BstSCI	CCNGG	6 cut(s)	531, 622, 1182, 2098, 2099, 2198
BstSFI	CTRYAG	6 cut(s)	504, 642, 717, 1083, 1716, 1749
BstSLI	GKGCMC	2 cut(s)	256, 402
BstSNI	TACGTA	1 cut(s)	445
BstUI	CGCG	4 cut(s)	649, 651, 1003, 2137
BstV1I	GCAGC	8 cut(s)	417, 656, 731, 1091, 1103, 1397, 1552, 1763
BstV2I	GAAGAC	2 cut(s)	720, 1144
BstXI	CCANNNNNNTGG	2 cut(s)	1542, 1796
BstZ17I	GTATAC	2 cut(s)	442, 1474
Bsu15I	ATCGAT	1 cut(s)	1776
BsuRI	GGCC	6 cut(s)	5, 490, 938, 1040, 1954, 2023
BsuTUI	ATCGAT	1 cut(s)	1776
BtgI	CCRYGG	2 cut(s)	54, 1872
BtrI	CACGTC	1 cut(s)	209
BtsCI	GGATG	7 cut(s)	144, 150, 524, 851, 996, 1469, 1647
BtsI	GCAGTG	1 cut(s)	1947
BtsIMutI	CAGTG	7 cut(s)	289, 498, 798, 992, 1947, 2113, 2180
CaiI	CAGNNNCTG	1 cut(s)	1715
CfoI	GCGC	5 cut(s)	242, 424, 649, 651, 1003
Cfr10I	RCCGGY	2 cut(s)	125, 1333
Cfr13I	GGNCC	6 cut(s)	626, 1039, 1301, 1402, 1899, 2022
Cfr9I	CCCGGG	1 cut(s)	2099
ClaI	ATCGAT	1 cut(s)	1776
CseI	GACGC	3 cut(s)	35, 1089, 2126
Csp6I	GTAC	7 cut(s)	139, 928, 1228, 1309, 1622, 1786, 1841
CspCI	CAANNNNNGTGG	2 cut(s)	36, 71
CviAII	CATG	8 cut(s)	155, 703, 925, 1429, 1483, 1801, 1873, 1889
CviQI	GTAC	7 cut(s)	139, 928, 1228, 1309, 1622, 1786, 1841
DpnI	GATC	8 cut(s)	111, 790, 1212, 1326, 1506, 1679, 1779, 1935
DpnII	GATC	8 cut(s)	109, 788, 1210, 1324, 1504, 1677, 1777, 1933
DraI	TTTAAA	1 cut(s)	382
EaeI	YGGCCR	1 cut(s)	1952
Eam1104I	CTCTTC	3 cut(s)	1167, 1513, 1956
EarI	CTCTTC	3 cut(s)	1167, 1513, 1956
EciI	GGCGGA	1 cut(s)	1130
Eco105I	TACGTA	1 cut(s)	445
Eco130I	CCWWGG	4 cut(s)	843, 1441, 1872, 2029
Eco31I	GGTCTC	1 cut(s)	1348
Eco32I	GATATC	1 cut(s)	1421
Eco47I	GGWCC	4 cut(s)	626, 1301, 1402, 1899
Eco88I	CYCGRG	1 cut(s)	2099
EcoO109I	RGGNCCY	1 cut(s)	1402
EcoRI	GAATTC	2 cut(s)	428, 1457
EcoRII	CCWGG	3 cut(s)	531, 622, 2198
EcoRV	GATATC	1 cut(s)	1421
EcoT14I	CCWWGG	4 cut(s)	843, 1441, 1872, 2029
ErhI	CCWWGG	4 cut(s)	843, 1441, 1872, 2029
Esp3I	CGTCTC	1 cut(s)	1543
FaeI	CATG	8 cut(s)	158, 706, 928, 1432, 1486, 1804, 1876, 1892
FalI	AAGNNNNNCTT	2 cut(s)	1008, 1040
FaqI	GGGAC	2 cut(s)	549, 1890
FatI	CATG	8 cut(s)	154, 702, 924, 1428, 1482, 1800, 1872, 1888
FbaI	TGATCA	1 cut(s)	109
FblI	GTMKAC	3 cut(s)	63, 441, 1473
Fnu4HI	GCNGC	9 cut(s)	406, 645, 720, 1080, 1092, 1411, 1566, 1752, 1755
FokI	GGATG	7 cut(s)	131, 137, 511, 838, 1003, 1476, 1634
Fsp4HI	GCNGC	9 cut(s)	406, 645, 720, 1080, 1092, 1411, 1566, 1752, 1755
FspBI	CTAG	4 cut(s)	825, 1469, 1694, 1970
GlaI	GCGC	5 cut(s)	241, 423, 648, 650, 1002
GluI	GCNGC	9 cut(s)	406, 645, 720, 1080, 1092, 1411, 1566, 1752, 1755
GsaI	CCCAGC	2 cut(s)	405, 557
GsuI	CTGGAG	1 cut(s)	2182
HaeII	RGCGCY	1 cut(s)	425
HaeIII	GGCC	6 cut(s)	5, 490, 938, 1040, 1954, 2023
HgaI	GACGC	3 cut(s)	35, 1089, 2126
HhaI	GCGC	5 cut(s)	242, 424, 649, 651, 1003
Hin1I	GRCGYC	2 cut(s)	27, 1100
Hin1II	CATG	8 cut(s)	158, 706, 928, 1432, 1486, 1804, 1876, 1892
Hin6I	GCGC	5 cut(s)	240, 422, 647, 649, 1001
HinP1I	GCGC	5 cut(s)	240, 422, 647, 649, 1001
HincII	GTYRAC	3 cut(s)	64, 471, 1806
HindII	GTYRAC	3 cut(s)	64, 471, 1806
HindIII	AAGCTT	2 cut(s)	1022, 1495
HinfI	GANTC	3 cut(s)	188, 848, 1268
HpaI	GTTAAC	1 cut(s)	1806
HphI	GGTGA	7 cut(s)	206, 1298, 1589, 1694, 1741, 1877, 2096
Hpy166II	GTNNAC	8 cut(s)	64, 442, 471, 1230, 1309, 1474, 1624, 1806
Hpy188I	TCNGA	8 cut(s)	22, 453, 672, 1131, 1463, 1819, 1924, 2207
Hpy188III	TCNNGA	6 cut(s)	855, 1405, 1781, 1906, 2151, 2174
Hpy8I	GTNNAC	8 cut(s)	64, 442, 471, 1230, 1309, 1474, 1624, 1806
Hpy99I	CGWCG	2 cut(s)	29, 1105
HpyAV	CCTTC	8 cut(s)	610, 1057, 1147, 1604, 1903, 2008, 2070, 2151
HpyCH4III	ACNGT	6 cut(s)	230, 284, 726, 1554, 1628, 2038
HpyCH4IV	ACGT	2 cut(s)	208, 444
HpySE526I	ACGT	2 cut(s)	208, 444
Hsp92I	GRCGYC	2 cut(s)	27, 1100
Hsp92II	CATG	8 cut(s)	158, 706, 928, 1432, 1486, 1804, 1876, 1892
HspAI	GCGC	5 cut(s)	240, 422, 647, 649, 1001
KroI	GCCGGC	1 cut(s)	125
KroNI	GCCGGC	1 cut(s)	127
Ksp22I	TGATCA	1 cut(s)	109
KspAI	GTTAAC	1 cut(s)	1806
Kzo9I	GATC	8 cut(s)	109, 788, 1210, 1324, 1504, 1677, 1777, 1933
LmnI	GCTCC	3 cut(s)	673, 2176, 2201
Lsp1109I	GCAGC	8 cut(s)	417, 656, 731, 1091, 1103, 1397, 1552, 1763
LweI	GCATC	7 cut(s)	172, 359, 981, 1351, 1454, 1721, 1917
MaeI	CTAG	4 cut(s)	825, 1469, 1694, 1970
MaeII	ACGT	2 cut(s)	208, 444
MaeIII	GTNAC	8 cut(s)	118, 764, 1523, 1628, 1729, 1769, 1942, 2084
MalI	GATC	8 cut(s)	111, 790, 1212, 1326, 1506, 1679, 1779, 1935
MboI	GATC	8 cut(s)	109, 788, 1210, 1324, 1504, 1677, 1777, 1933
MfeI	CAATTG	2 cut(s)	316, 1956
MhlI	GDGCHC	3 cut(s)	256, 402, 670
MlsI	TGGCCA	1 cut(s)	1954
MluNI	TGGCCA	1 cut(s)	1954
MlyI	GAGTC	1 cut(s)	842
MmeI	TCCRAC	4 cut(s)	666, 1411, 2141, 2185
Mox20I	TGGCCA	1 cut(s)	1954
MroNI	GCCGGC	1 cut(s)	125
MroXI	GAANNNNTTC	2 cut(s)	1382, 1918
MscI	TGGCCA	1 cut(s)	1954
MseI	TTAA	8 cut(s)	381, 498, 602, 693, 833, 896, 1805, 2063
MslI	CAYNNNNRTG	2 cut(s)	1540, 1993
Msp20I	TGGCCA	1 cut(s)	1954
MspA1I	CMGCKG	1 cut(s)	1094
MspR9I	CCNGG	6 cut(s)	533, 624, 1184, 2100, 2101, 2200
MunI	CAATTG	2 cut(s)	316, 1956
Mva1269I	GAATGC	3 cut(s)	367, 757, 1198
MvaI	CCWGG	3 cut(s)	533, 624, 2200
MvnI	CGCG	4 cut(s)	649, 651, 1003, 2137
NaeI	GCCGGC	1 cut(s)	127
NciI	CCSGG	3 cut(s)	1184, 2100, 2101
NcoI	CCATGG	1 cut(s)	1872
NdeII	GATC	8 cut(s)	109, 788, 1210, 1324, 1504, 1677, 1777, 1933
NgoMIV	GCCGGC	1 cut(s)	125
NheI	GCTAGC	1 cut(s)	1693
NlaIII	CATG	8 cut(s)	158, 706, 928, 1432, 1486, 1804, 1876, 1892
NlaIV	GGNNCC	5 cut(s)	130, 621, 1447, 1614, 2178
NmeAIII	GCCGAG	2 cut(s)	914, 935
NmuCI	GTSAC	5 cut(s)	764, 1628, 1729, 1942, 2084
NspI	RCATGY	2 cut(s)	928, 1804
NspV	TTCGAA	1 cut(s)	749
OliI	CACNNNNGTG	1 cut(s)	1540
PasI	CCCWGGG	1 cut(s)	532
PauI	GCGCGC	1 cut(s)	647
PciI	ACATGT	1 cut(s)	924
PctI	GAATGC	3 cut(s)	367, 757, 1198
PdiI	GCCGGC	1 cut(s)	127
PdmI	GAANNNNTTC	2 cut(s)	1382, 1918
PfeI	GAWTC	2 cut(s)	188, 1268
PflMI	CCANNNNNTGG	1 cut(s)	785
PfoI	TCCNGGA	1 cut(s)	622
PkrI	GCNGC	9 cut(s)	407, 646, 721, 1081, 1093, 1412, 1567, 1753, 1756
PleI	GAGTC	1 cut(s)	842
PpsI	GAGTC	1 cut(s)	842
Ppu21I	YACGTR	1 cut(s)	445
PpuMI	RGGWCCY	1 cut(s)	1402
PscI	ACATGT	1 cut(s)	924
PshBI	ATTAAT	1 cut(s)	693
Psp5II	RGGWCCY	1 cut(s)	1402
Psp6I	CCWGG	3 cut(s)	531, 622, 2198
PspFI	CCCAGC	2 cut(s)	401, 553
PspGI	CCWGG	3 cut(s)	531, 622, 2198
PspN4I	GGNNCC	5 cut(s)	130, 621, 1447, 1614, 2178
PspPI	GGNCC	6 cut(s)	626, 1039, 1301, 1402, 1899, 2022
PspPPI	RGGWCCY	1 cut(s)	1402
PsrI	GAACNNNNNNTAC	4 cut(s)	1605, 1637, 2146, 2178
PstI	CTGCAG	4 cut(s)	508, 646, 721, 1753
PstNI	CAGNNNCTG	1 cut(s)	1715
PteI	GCGCGC	1 cut(s)	647
PvuII	CAGCTG	1 cut(s)	1094
RsaI	GTAC	7 cut(s)	140, 929, 1229, 1310, 1623, 1787, 1842
RsaNI	GTAC	7 cut(s)	139, 928, 1228, 1309, 1622, 1786, 1841
RseI	CAYNNNNRTG	2 cut(s)	1540, 1993
SalI	GTCGAC	1 cut(s)	62
SaqAI	TTAA	8 cut(s)	381, 498, 602, 693, 833, 896, 1805, 2063
SatI	GCNGC	9 cut(s)	406, 645, 720, 1080, 1092, 1411, 1566, 1752, 1755
Sau3AI	GATC	8 cut(s)	109, 788, 1210, 1324, 1504, 1677, 1777, 1933
Sau96I	GGNCC	6 cut(s)	626, 1039, 1301, 1402, 1899, 2022
ScaI	AGTACT	1 cut(s)	1842
SchI	GAGTC	1 cut(s)	842
ScrFI	CCNGG	6 cut(s)	533, 624, 1184, 2100, 2101, 2200
SduI	GDGCHC	3 cut(s)	256, 402, 670
SfaNI	GCATC	7 cut(s)	172, 359, 981, 1351, 1454, 1721, 1917
SfcI	CTRYAG	6 cut(s)	504, 642, 717, 1083, 1716, 1749
SfuI	TTCGAA	1 cut(s)	749
SinI	GGWCC	4 cut(s)	626, 1301, 1402, 1899
SmaI	CCCGGG	1 cut(s)	2101
SmiMI	CAYNNNNRTG	2 cut(s)	1540, 1993
SmlI	CTYRAG	1 cut(s)	1368
SmoI	CTYRAG	1 cut(s)	1368
SnaBI	TACGTA	1 cut(s)	445
SsiI	CCGC	5 cut(s)	51, 123, 742, 1115, 1755
SspI	AATATT	1 cut(s)	334
SspMI	CTAG	4 cut(s)	825, 1469, 1694, 1970
StyD4I	CCNGG	6 cut(s)	531, 622, 1182, 2098, 2099, 2198
StyI	CCWWGG	4 cut(s)	843, 1441, 1872, 2029
TaaI	ACNGT	6 cut(s)	230, 284, 726, 1554, 1628, 2038
TaiI	ACGT	2 cut(s)	211, 447
TaqI	TCGA	8 cut(s)	63, 749, 816, 903, 1011, 1323, 1776, 1905
TaqII	GACCGA	1 cut(s)	1961
TatI	WGTACW	5 cut(s)	927, 1227, 1308, 1621, 1840
TauI	GCSGC	1 cut(s)	1757
TfiI	GAWTC	2 cut(s)	188, 1268
Tru1I	TTAA	8 cut(s)	381, 498, 602, 693, 833, 896, 1805, 2063
Tru9I	TTAA	8 cut(s)	381, 498, 602, 693, 833, 896, 1805, 2063
TscAI	CASTG	7 cut(s)	289, 498, 798, 992, 1947, 2113, 2187
TseFI	GTSAC	5 cut(s)	764, 1628, 1729, 1942, 2084
TseI	GCWGC	8 cut(s)	405, 644, 719, 1079, 1091, 1410, 1565, 1751
Tsp45I	GTSAC	5 cut(s)	764, 1628, 1729, 1942, 2084
TspDTI	ATGAA	4 cut(s)	582, 774, 1166, 1577
TspGWI	ACGGA	3 cut(s)	594, 1271, 1506
TspMI	CCCGGG	1 cut(s)	2099
TspRI	CASTG	7 cut(s)	289, 498, 798, 992, 1947, 2113, 2187
Van91I	CCANNNNNTGG	1 cut(s)	785
VpaK11BI	GGWCC	4 cut(s)	626, 1301, 1402, 1899
VspI	ATTAAT	1 cut(s)	693
XapI	RAATTY	6 cut(s)	428, 569, 835, 1261, 1457, 1572
XceI	RCATGY	2 cut(s)	928, 1804
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1870
XmaI	CCCGGG	1 cut(s)	2099
XmiI	GTMKAC	3 cut(s)	63, 441, 1473
XmnI	GAANNNNTTC	2 cut(s)	1382, 1918
XspI	CTAG	4 cut(s)	825, 1469, 1694, 1970
ZrmI	AGTACT	1 cut(s)	1842

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.