MD08G1066700.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012544
Physical Location & Seq
Reverse (-)
5338289 .. 5342543
4255 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1066700.v1.1.491

Sequence Viewer

Length: 2652 bp
ATGCTCCTAAGACAACAAGTTGCAGGGATACGACGTGGCTTTCATTTGAACATTTACACATTTTCCAAAAGGTTTTCATCCTTGTCAAATGAGCCTCTGTCTGAAGAGAAAGTTGTATCTTTGATTGATGTGAGACAAGTAGTTGATGATGTGTGCCGAGTCTTGGAGTGTGGTCCTTGGGGACCCACTCTTGAGAATGCTCTATCGGCGCTTGACAAAACACCTCAATCAGAATCAGTTATTGGAGTTTTAAGGAGGTTGAAAGATGTCAGTTTAGCACTTAATTATTTTCGGTGGTGTGAGAAACAAACTGACCAAGCACACTGTCCCGAAGCATACAATTCACTTCTCATGGTCATGGCCAGGAGTAGAAATCTTAATTATTTGGAACAGGTCCTGGAAGAAATGAGTGTAGCAGGAATTGGCCCATTTAATACCACATGTATTGAGTTAGTTGTGAGCTGTATGAGGTCACAGAAGCTGAAAGATGGTTTCAATCTACTGCAAACAATGAGGAAATTCAAATTCCGCCCAGCATTTTCAGCCTATACAACTCTTATTGGGGCTTTGTCTACAGTTCCTGAATCTGATCTCATGTTGACTCTCTTTCATCAAATGCAGGAGCTAGGTTATGAAGTTACTGTGCATTTATTTACAACCATTATTCGTGTATTTGCCAAGGAAGGACGAGTTGATGCTGCCCTCTCCTTGTTGGATGAGATGAAGAGCAACTCTTTTAGTGCAGATGTTGTCCTTTATAATGTCTGTATAGATTGCTTTGGCAAGGTCGGTAAGGTGGATATGGCCTGGAAATTCTTTCATGAAATGAGAATGCATGGCTTGGTGCCTGATGATGTGACATATACTAGCATGATAGGAGTTCTCTGCCAAGCTGCGAGACTAGACGAAGCAGTGGAGCTATTTGAGGAGATGGACAGACATAGGAAAGTACCTTGTGTGTATGCGTATAACACCATGATCATGGGCTATGGCTCTGCTGGAAGGTTTGAGGAAGCATACAGCTTGTTTGAGAGGCAAAGAAGAAGGGGGTGCATTCCGAGTGTGATTGCATATAACTGTGTTCTCACCTGCCTAGGGAAGAAGAGACGAGCAGTAGAAGCATTAAGGGTTTTTGATGAGATGAAGAAAGATGCAGCTCCCAATCTTCCAACTTATAACATTCTAATAGACATGCTTTGTAAGGCTGGTAACCTCAAGGATGCTCTGCAGGTTCGGGATGCCATGAAAGAAGCTGGCTTATATCCTAATGTTATGACTGTAAACATAATGATTGATCGACTCTGTAAAGCTCAAAAACTTGATGATGCTTGTTCTATATTTGAAGGAATGGATCACAAAGTCTGCACACCAGATGCAGTTACATTTTGTTCTCTTATAGATGGCTTGGGAAAACAAGGAAGAGTAGATGATGCCTATAGGCTTTATGAAAAGATGCTGGACGCTGATCAGACTCCAAATGCCATTGTATACACATCCCTCATCAGGAACTTCTTCAAATGTGGCAGGAAGGAGGATGGTCACAAAATATATAAAGAAATGCAGCGTAGGGGCTGTTCTCCTGATCTCATGCTTCTTAATACCTATATGGATTGTGTTTTAAAGGCCGGTGAAATTGAAAAAGGTAGATCTTTGTTTGAGGAGATTAAGACTCAAGGATTCATTCCGGATGTGCGGAGTTATTCAATCTTAATTCATGGCCTTGTAAAAGCAGGCTTTGCACATCAAACATATGAATTATACTATGCGATGAAAGATCAAGGCTGTGTATTGGACACCCGTGCTTACAACGCCATCATTGATGGATTCTGCAAAAGTGGAAAGGTGAATAAAGCATACCAGCTTCTGGAGGAAATGAAAACTAAGGGTCACCAACCCACCGTCGTTACTTATGGTTCTGTCATTGATGGTCTTGCCAAGATTGACAGGCTTGATGAAGCATACATGCTTTTTGAAGAAGCTAAGTCAAAAGGAGTAGAACTGAATGTAATCATTTATAGTAGCCTTGTTGATGGGTTTGGGAAGGTAGGTAGAATAGATGAAGCATATCTAATCATGGAAGAGTTGATGCAGAAAGGTTTGTCACCTAACGTTTACACATATAATAGCTTGCTTGATGGACTTGTGAAAGCTGGTGAAATTGATGAAGCAATTGTCTGCTTTCAATCGATGAAAGATATGAAATGTACACCCAACCATGTAACGTATAGCATTCTCATAAATGGTCTTTGTAGGGTTAGAAAATTCAATAAGGCTTTTGTATACTGGCAAGAGATGAAGAAACAAGGGTTAAACCCTAATACAATCACCTACACTACCATGATCTCTGGACTTACAAGGGCTGGGAACATAAGGGATGCTAGTGGGCTTTTTGAGAAGTTTAAGGCCAGTGGTGGCATTCCCGATTCTGCTTGTTATAATGCTATGATAGAAGGGTTGAGCAATGCAGAAAGGGCAATGGACGCGTATACACTTTTTGAGGAAACTCGGCGGAAAGGTTGTAATATTCATTCCAAGACATGTGTTGTTCTGTTGGATGCCTTGCACAAGGCTGAATGCCTTGAGCAGGCAGGAATTGTTGGTGCTGTTCTTCGAGAAACTGCAAAGTCTCAGCATGCATCAAGATCATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

884

Amino Acids

99.52

Weight (kDa)

7.4

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 202 - 262 4e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 210 - 242 6e-06 PPR repeat
PPR_2 PF13041 215 - 251 2.4e-06 PPR repeat family
PPR_long PF17177 226 - 342 3.5e-07 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 226 - 344 1.2e-08 Fungal tetratrico peptide repeats
PPR_2 PF13041 249 - 297 4e-16 PPR repeat family
PPR PF01535 251 - 281 2.7e-06 PPR repeat
PPR_1 PF12854 280 - 311 7e-12 PPR repeat
PPR_2 PF13041 283 - 314 1.1e-06 PPR repeat family
PPR PF01535 286 - 314 2.9e-07 PPR repeat
PPR_2 PF13041 318 - 355 9.2e-08 PPR repeat family
PPR PF01535 322 - 351 6e-07 PPR repeat
PPR_3 PF13812 342 - 399 1.3e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 353 - 401 5.2e-13 PPR repeat family
TPR_24 PF23276 361 - 484 1.7e-08 Fungal tetratrico peptide repeats
PPR_long PF17177 362 - 511 1.4e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 376 - 432 6.9e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 386 - 416 4.2e-10 PPR repeat
PPR_2 PF13041 387 - 436 1e-14 PPR repeat family
PPR PF01535 391 - 420 1.3e-06 PPR repeat
PPR_1 PF12854 419 - 450 7.8e-08 PPR repeat
PPR_2 PF13041 422 - 471 2e-12 PPR repeat family
PPR_1 PF12854 455 - 485 2.2e-10 PPR repeat
PPR PF01535 460 - 487 8.7e-06 PPR repeat
PPR_2 PF13041 464 - 503 7.8e-08 PPR repeat family
PPR_2 PF13041 492 - 528 5.4e-08 PPR repeat family
PPR_long PF17177 512 - 637 2.5e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 527 - 576 2.1e-09 PPR repeat family
PPR_3 PF13812 589 - 641 1.1e-07 Pentatricopeptide repeat domain
TPR_24 PF23276 590 - 688 1.9e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 594 - 626 8.6e-13 PPR repeat
PPR_2 PF13041 598 - 646 2.5e-17 PPR repeat family
PPR PF01535 601 - 629 2e-08 PPR repeat
PPR_1 PF12854 628 - 659 6.1e-08 PPR repeat
PPR_2 PF13041 632 - 681 3.4e-10 PPR repeat family
PPR_long PF17177 646 - 724 4.6e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 656 - 711 8.5e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 663 - 694 1e-06 PPR repeat
PPR_2 PF13041 668 - 716 1.8e-12 PPR repeat family
PPR_3 PF13812 691 - 747 3.2e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 698 - 731 2.7e-09 PPR repeat
PPR_2 PF13041 702 - 751 3.3e-19 PPR repeat family
PPR PF01535 705 - 735 9.5e-07 PPR repeat
PPR_long PF17177 721 - 850 1.3e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 734 - 766 2.2e-09 PPR repeat
PPR PF01535 740 - 770 1.4e-06 PPR repeat
PPR_2 PF13041 745 - 785 6.5e-13 PPR repeat family
PPR_3 PF13812 763 - 817 3.8e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 769 - 799 4.8e-09 PPR repeat
PPR_2 PF13041 773 - 821 2.1e-09 PPR repeat family
PPR_2 PF13041 807 - 856 6.4e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012544)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06920 AT3G06920 AT3G06920
fragaria_vesca FvH4_4g18230 FvH4_4g18230
malus_domestica MD08G1066700.v1.1
prunus_persica Prupe.1G135500_v2.0.a1
pyrus_communis pycom13g14370
rosa_chinensis RchiOBHm_Chr4g0422371
rosa_laevigata RLG00000007615
rosa_multiflora Rmu_sc0013245.1_g000002
rosa_roxburghii Rroxscaffold_5G00364490
rosa_rugosa Rorug04G0179500
rosa_samantha Rh4AG239000 Rh4AG239400 Rh4BG242600 Rh4CG254000 Rh4DG238100
rosa_wichuraiana Rw4G020660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 759, 1176, 2436
AarI CACCTGC 1 cut(s) 1097
Acc36I ACCTGC 2 cut(s) 1097, 1219
AccB1I GGYRCC 1 cut(s) 844
AccB7I CCANNNNNTGG 1 cut(s) 1864
AccI GTMKAC 4 cut(s) 572, 1488, 2280, 2486
AccII CGCG 1 cut(s) 2483
AccIII TCCGGA 1 cut(s) 1684
AciI CCGC 3 cut(s) 529, 1693, 2509
AclI AACGTT 1 cut(s) 2109
AclWI GGATC 1 cut(s) 1359
AcoI YGGCCR 1 cut(s) 360
AcsI RAATTY 4 cut(s) 518, 524, 812, 2261
AcuI CTGAAG 1 cut(s) 123
AfaI GTAC 2 cut(s) 951, 2206
AfiI CCNNNNNNNGG 5 cut(s) 163, 1095, 1503, 1864, 2584
AflIII ACRYGT 3 cut(s) 440, 2481, 2537
AjiI CACGTC 1 cut(s) 35
AjnI CCWGG 3 cut(s) 362, 396, 806
AjuI GAANNNNNNNTTGG 2 cut(s) 225, 257
Alw26I GTCTC 4 cut(s) 127, 892, 1099, 2631
AlwI GGATC 1 cut(s) 1359
AlwNI CAGNNNCTG 4 cut(s) 397, 481, 581, 1864
Aor13HI TCCGGA 1 cut(s) 1684
AoxI GGCC 6 cut(s) 360, 424, 804, 1623, 1717, 2403
ApeKI GCWGC 4 cut(s) 698, 893, 1154, 1561
ApoI RAATTY 4 cut(s) 518, 524, 812, 2261
Asp700I GAANNNNTTC 1 cut(s) 1511
AspA2I CCTAGG 1 cut(s) 1093
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 4 cut(s) 173, 182, 394, 425
AsuHPI GGTGA 7 cut(s) 1078, 1640, 1855, 1880, 2094, 2165, 2317
AvaII GGWCC 3 cut(s) 173, 182, 394
AvrII CCTAGG 1 cut(s) 1093
BalI TGGCCA 1 cut(s) 362
BanI GGYRCC 1 cut(s) 844
BbvI GCAGC 4 cut(s) 685, 880, 1166, 1573
BccI CCATC 9 cut(s) 482, 925, 1394, 1529, 1814, 1820, 1919, 2024, 2129
BciT130I CCWGG 3 cut(s) 364, 398, 808
BciVI GTATCC 1 cut(s) 21
BclI TGATCA 2 cut(s) 978, 1465
BcoDI GTCTC 4 cut(s) 127, 892, 1099, 2631
BfaI CTAG 5 cut(s) 626, 867, 902, 1094, 2379
BfmI CTRYAG 3 cut(s) 573, 1226, 1435
BfoI RGCGCY 1 cut(s) 212
BfuAI ACCTGC 2 cut(s) 1097, 1219
BfuI GTATCC 1 cut(s) 21
BglII AGATCT 1 cut(s) 1646
BisI GCNGC 4 cut(s) 699, 894, 1155, 1562
BlnI CCTAGG 1 cut(s) 1093
BlsI GCNGC 4 cut(s) 700, 895, 1156, 1563
Bme1390I CCNGG 3 cut(s) 364, 398, 808
Bme18I GGWCC 3 cut(s) 173, 182, 394
BmgBI CACGTC 1 cut(s) 35
BmgT120I GGNCC 4 cut(s) 173, 182, 394, 425
BmiI GGNNCC 3 cut(s) 183, 184, 846
BmrFI CCNGG 3 cut(s) 364, 398, 808
BpmI CTGGAG 1 cut(s) 1886
BpuEI CTTGAG 4 cut(s) 212, 1199, 1656, 2600
Bsa29I ATCGAT 1 cut(s) 2186
BsaJI CCNNGG 3 cut(s) 176, 678, 1093
BsaWI WCCGGW 1 cut(s) 1684
Bsc4I CCNNNNNNNGG 5 cut(s) 163, 1095, 1503, 1864, 2584
Bse118I RCCGGY 1 cut(s) 1625
Bse1I ACTGG 2 cut(s) 2288, 2406
Bse3DI GCAATG 2 cut(s) 2467, 2481
BseAI TCCGGA 1 cut(s) 1684
BseBI CCWGG 3 cut(s) 364, 398, 808
BseCI ATCGAT 1 cut(s) 2186
BseDI CCNNGG 3 cut(s) 176, 678, 1093
BseGI GGATG 9 cut(s) 77, 721, 1225, 1243, 1493, 1540, 1693, 2380, 2560
BseLI CCNNNNNNNGG 5 cut(s) 163, 1095, 1503, 1864, 2584
BseMI GCAATG 2 cut(s) 2467, 2481
BseMII CTCAG 1 cut(s) 2642
BseNI ACTGG 2 cut(s) 2288, 2406
BseRI GAGGAG 2 cut(s) 941, 1673
BseXI GCAGC 4 cut(s) 685, 880, 1166, 1573
BseYI CCCAGC 2 cut(s) 532, 2360
BsgI GTGCAG 2 cut(s) 762, 1348
Bsh1236I CGCG 1 cut(s) 2483
BshFI GGCC 6 cut(s) 362, 426, 806, 1625, 1719, 2405
BshNI GGYRCC 1 cut(s) 844
BshVI ATCGAT 1 cut(s) 2186
BsiSI CCGG 2 cut(s) 1626, 1685
BslFI GGGAC 2 cut(s) 195, 312
BslI CCNNNNNNNGG 5 cut(s) 163, 1095, 1503, 1864, 2584
BsmAI GTCTC 4 cut(s) 127, 892, 1099, 2631
BsmBI CGTCTC 1 cut(s) 1099
BsmFI GGGAC 2 cut(s) 195, 312
BsmI GAATGC 6 cut(s) 202, 837, 1053, 2229, 2415, 2579
BsnI GGCC 6 cut(s) 362, 426, 806, 1625, 1719, 2405
Bsp13I TCCGGA 1 cut(s) 1684
Bsp1407I TGTACA 1 cut(s) 2204
BspACI CCGC 3 cut(s) 529, 1693, 2509
BspANI GGCC 6 cut(s) 362, 426, 806, 1625, 1719, 2405
BspCNI CTCAG 1 cut(s) 2641
BspDI ATCGAT 1 cut(s) 2186
BspEI TCCGGA 1 cut(s) 1684
BspFNI CGCG 1 cut(s) 2483
BspHI TCATGA 1 cut(s) 820
BspLI GGNNCC 3 cut(s) 183, 184, 846
BspMAI CTGCAG 1 cut(s) 1230
BspMI ACCTGC 2 cut(s) 1097, 1219
BspPI GGATC 1 cut(s) 1359
BspQI GCTCTTC 1 cut(s) 719
BspT107I GGYRCC 1 cut(s) 844
BsrDI GCAATG 2 cut(s) 2467, 2481
BsrFI RCCGGY 1 cut(s) 1625
BsrGI TGTACA 1 cut(s) 2204
BsrI ACTGG 2 cut(s) 2288, 2406
BssAI RCCGGY 1 cut(s) 1625
BssECI CCNNGG 3 cut(s) 176, 678, 1093
BssNAI GTATAC 3 cut(s) 1489, 2281, 2487
BssT1I CCWWGG 3 cut(s) 176, 678, 1093
Bst1107I GTATAC 3 cut(s) 1489, 2281, 2487
Bst2UI CCWGG 3 cut(s) 364, 398, 808
Bst4CI ACNGT 6 cut(s) 326, 577, 643, 1079, 1279, 1900
Bst6I CTCTTC 5 cut(s) 99, 719, 1097, 1414, 2073
BstAPI GCANNNNNTGC 1 cut(s) 1736
BstAUI TGTACA 1 cut(s) 2204
BstC8I GCNNGC 5 cut(s) 1255, 1732, 2129, 2586, 2634
BstDEI CTNAG 4 cut(s) 8, 1881, 1980, 2628
BstEII GGTNACC 2 cut(s) 1208, 1886
BstENI CCTNNNNNAGG 1 cut(s) 2582
BstF5I GGATG 9 cut(s) 77, 721, 1225, 1243, 1493, 1540, 1693, 2380, 2560
BstFNI CGCG 1 cut(s) 2483
BstH2I RGCGCY 1 cut(s) 212
BstHHI GCGC 1 cut(s) 211
BstMAI GTCTC 4 cut(s) 127, 892, 1099, 2631
BstMWI GCNNNNNNNGC 7 cut(s) 206, 542, 1118, 1736, 1808, 2471, 2480
BstNI CCWGG 3 cut(s) 364, 398, 808
BstNSI RCATGY 5 cut(s) 444, 1195, 1966, 2541, 2636
BstPI GGTNACC 2 cut(s) 1208, 1886
BstSCI CCNGG 3 cut(s) 362, 396, 806
BstSFI CTRYAG 3 cut(s) 573, 1226, 1435
BstUI CGCG 1 cut(s) 2483
BstV1I GCAGC 4 cut(s) 685, 880, 1166, 1573
BstX2I RGATCY 1 cut(s) 1646
BstXI CCANNNNNNTGG 1 cut(s) 982
BstYI RGATCY 1 cut(s) 1646
BstZ17I GTATAC 3 cut(s) 1489, 2281, 2487
Bsu15I ATCGAT 1 cut(s) 2186
BsuI GTATCC 1 cut(s) 21
BsuRI GGCC 6 cut(s) 362, 426, 806, 1625, 1719, 2405
BsuTUI ATCGAT 1 cut(s) 2186
BtgZI GCGATG 1 cut(s) 1781
BtrI CACGTC 1 cut(s) 35
BtsCI GGATG 9 cut(s) 77, 721, 1225, 1243, 1493, 1540, 1693, 2380, 2560
BtsI GCAGTG 1 cut(s) 918
BtsIMutI CAGTG 3 cut(s) 322, 918, 2413
BveI ACCTGC 2 cut(s) 1097, 1219
Cac8I GCNNGC 5 cut(s) 1255, 1732, 2129, 2586, 2634
CaiI CAGNNNCTG 4 cut(s) 397, 481, 581, 1864
CciI TCATGA 1 cut(s) 820
CfoI GCGC 1 cut(s) 211
Cfr10I RCCGGY 1 cut(s) 1625
Cfr13I GGNCC 4 cut(s) 173, 182, 394, 425
ClaI ATCGAT 1 cut(s) 2186
CseI GACGC 2 cut(s) 1469, 2489
Csp6I GTAC 2 cut(s) 950, 2205
CspCI CAANNNNNGTGG 2 cut(s) 427, 462
CviQI GTAC 2 cut(s) 950, 2205
DdeI CTNAG 4 cut(s) 8, 1881, 1980, 2628
DraI TTTAAA 1 cut(s) 1620
EaeI YGGCCR 1 cut(s) 360
Eam1104I CTCTTC 5 cut(s) 99, 719, 1097, 1414, 2073
EarI CTCTTC 5 cut(s) 99, 719, 1097, 1414, 2073
EciI GGCGGA 2 cut(s) 518, 2524
Eco130I CCWWGG 3 cut(s) 176, 678, 1093
Eco47I GGWCC 3 cut(s) 173, 182, 394
Eco57I CTGAAG 1 cut(s) 123
Eco91I GGTNACC 2 cut(s) 1208, 1886
EcoNI CCTNNNNNAGG 1 cut(s) 2582
EcoO109I RGGNCCY 2 cut(s) 182, 394
EcoO65I GGTNACC 2 cut(s) 1208, 1886
EcoRII CCWGG 3 cut(s) 362, 396, 806
EcoT14I CCWWGG 3 cut(s) 176, 678, 1093
EcoT22I ATGCAT 2 cut(s) 837, 2638
ErhI CCWWGG 3 cut(s) 176, 678, 1093
Esp3I CGTCTC 1 cut(s) 1099
FaqI GGGAC 2 cut(s) 195, 312
FauNDI CATATG 1 cut(s) 1750
FbaI TGATCA 2 cut(s) 978, 1465
FblI GTMKAC 4 cut(s) 572, 1488, 2280, 2486
Fnu4HI GCNGC 4 cut(s) 699, 894, 1155, 1562
FokI GGATG 9 cut(s) 64, 728, 1232, 1250, 1480, 1547, 1700, 2387, 2567
Fsp4HI GCNGC 4 cut(s) 699, 894, 1155, 1562
FspBI CTAG 5 cut(s) 626, 867, 902, 1094, 2379
GlaI GCGC 1 cut(s) 210
GluI GCNGC 4 cut(s) 699, 894, 1155, 1562
GsaI CCCAGC 2 cut(s) 536, 2364
GsuI CTGGAG 1 cut(s) 1886
HaeII RGCGCY 1 cut(s) 212
HaeIII GGCC 6 cut(s) 362, 426, 806, 1625, 1719, 2405
HapII CCGG 2 cut(s) 1626, 1685
HgaI GACGC 2 cut(s) 1469, 2489
HhaI GCGC 1 cut(s) 211
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HincII GTYRAC 1 cut(s) 600
HindII GTYRAC 1 cut(s) 600
HpaII CCGG 2 cut(s) 1626, 1685
HphI GGTGA 7 cut(s) 1078, 1640, 1855, 1880, 2094, 2165, 2317
Hpy166II GTNNAC 8 cut(s) 573, 600, 1282, 1489, 2113, 2207, 2281, 2487
Hpy188I TCNGA 5 cut(s) 103, 232, 589, 1059, 1470
Hpy8I GTNNAC 8 cut(s) 573, 600, 1282, 1489, 2113, 2207, 2281, 2487
Hpy99I CGWCG 2 cut(s) 36, 1904
HpyAV CCTTC 7 cut(s) 677, 996, 1038, 1337, 1522, 2035, 2444
HpyCH4III ACNGT 6 cut(s) 326, 577, 643, 1079, 1279, 1900
HpyCH4IV ACGT 3 cut(s) 34, 2109, 2222
HpyF10VI GCNNNNNNNGC 7 cut(s) 206, 542, 1118, 1736, 1808, 2471, 2480
HpyF3I CTNAG 4 cut(s) 8, 1881, 1980, 2628
HpySE526I ACGT 3 cut(s) 34, 2109, 2222
HspAI GCGC 1 cut(s) 209
KflI GGGWCCC 1 cut(s) 182
Kpn2I TCCGGA 1 cut(s) 1684
Ksp22I TGATCA 2 cut(s) 978, 1465
LguI GCTCTTC 1 cut(s) 719
LmnI GCTCC 4 cut(s) 9, 622, 916, 1162
Lsp1109I GCAGC 4 cut(s) 685, 880, 1166, 1573
MaeI CTAG 5 cut(s) 626, 867, 902, 1094, 2379
MaeII ACGT 3 cut(s) 34, 2109, 2222
MfeI CAATTG 1 cut(s) 2169
MflI RGATCY 1 cut(s) 1646
MlsI TGGCCA 1 cut(s) 362
MluI ACGCGT 1 cut(s) 2481
MluNI TGGCCA 1 cut(s) 362
MlyI GAGTC 5 cut(s) 168, 595, 1293, 1465, 1663
MmeI TCCRAC 3 cut(s) 693, 1193, 2532
Mox20I TGGCCA 1 cut(s) 362
Mph1103I ATGCAT 2 cut(s) 837, 2638
MroI TCCGGA 1 cut(s) 1684
MroXI GAANNNNTTC 1 cut(s) 1511
MscI TGGCCA 1 cut(s) 362
MslI CAYNNNNRTG 3 cut(s) 356, 980, 2336
Msp20I TGGCCA 1 cut(s) 362
MspI CCGG 2 cut(s) 1626, 1685
MspR9I CCNGG 3 cut(s) 364, 398, 808
MunI CAATTG 1 cut(s) 2169
Mva1269I GAATGC 6 cut(s) 202, 837, 1053, 2229, 2415, 2579
MvaI CCWGG 3 cut(s) 364, 398, 808
MvnI CGCG 1 cut(s) 2483
MwoI GCNNNNNNNGC 7 cut(s) 206, 542, 1118, 1736, 1808, 2471, 2480
NdeI CATATG 1 cut(s) 1750
NlaIV GGNNCC 3 cut(s) 183, 184, 846
NmeAIII GCCGAG 2 cut(s) 182, 2485
NmuCI GTSAC 5 cut(s) 471, 856, 1538, 1886, 2100
NsiI ATGCAT 2 cut(s) 837, 2638
NspI RCATGY 5 cut(s) 444, 1195, 1966, 2541, 2636
PaeI GCATGC 1 cut(s) 2636
PagI TCATGA 1 cut(s) 820
PaqCI CACCTGC 1 cut(s) 1097
PciI ACATGT 2 cut(s) 440, 2537
PciSI GCTCTTC 1 cut(s) 719
PctI GAATGC 6 cut(s) 202, 837, 1053, 2229, 2415, 2579
PdmI GAANNNNTTC 1 cut(s) 1511
PfeI GAWTC 5 cut(s) 233, 584, 1677, 1824, 2423
PflMI CCANNNNNTGG 1 cut(s) 1864
PfoI TCCNGGA 1 cut(s) 396
PkrI GCNGC 4 cut(s) 700, 895, 1156, 1563
PleI GAGTC 5 cut(s) 167, 595, 1293, 1465, 1663
PpsI GAGTC 5 cut(s) 167, 595, 1293, 1465, 1663
PpuMI RGGWCCY 2 cut(s) 182, 394
PscI ACATGT 2 cut(s) 440, 2537
PsiI TTATAA 3 cut(s) 759, 1176, 2436
Psp1406I AACGTT 1 cut(s) 2109
Psp5II RGGWCCY 2 cut(s) 182, 394
Psp6I CCWGG 3 cut(s) 362, 396, 806
PspEI GGTNACC 2 cut(s) 1208, 1886
PspFI CCCAGC 2 cut(s) 532, 2360
PspGI CCWGG 3 cut(s) 362, 396, 806
PspN4I GGNNCC 3 cut(s) 183, 184, 846
PspPI GGNCC 4 cut(s) 173, 182, 394, 425
PspPPI RGGWCCY 2 cut(s) 182, 394
PsrI GAACNNNNNNTAC 2 cut(s) 1558, 1590
PstI CTGCAG 1 cut(s) 1230
PstNI CAGNNNCTG 4 cut(s) 397, 481, 581, 1864
PsuI RGATCY 1 cut(s) 1646
RsaI GTAC 2 cut(s) 951, 2206
RsaNI GTAC 2 cut(s) 950, 2205
RseI CAYNNNNRTG 3 cut(s) 356, 980, 2336
SapI GCTCTTC 1 cut(s) 719
SatI GCNGC 4 cut(s) 699, 894, 1155, 1562
Sau96I GGNCC 4 cut(s) 173, 182, 394, 425
SchI GAGTC 5 cut(s) 168, 595, 1293, 1465, 1663
ScrFI CCNGG 3 cut(s) 364, 398, 808
SfcI CTRYAG 3 cut(s) 573, 1226, 1435
SinI GGWCC 3 cut(s) 173, 182, 394
SmiMI CAYNNNNRTG 3 cut(s) 356, 980, 2336
SmlI CTYRAG 4 cut(s) 191, 1214, 1671, 2579
SmoI CTYRAG 4 cut(s) 191, 1214, 1671, 2579
SphI GCATGC 1 cut(s) 2636
SsiI CCGC 3 cut(s) 529, 1693, 2509
SspI AATATT 1 cut(s) 2524
SspMI CTAG 5 cut(s) 626, 867, 902, 1094, 2379
StyD4I CCNGG 3 cut(s) 362, 396, 806
StyI CCWWGG 3 cut(s) 176, 678, 1093
TaaI ACNGT 6 cut(s) 326, 577, 643, 1079, 1279, 1900
TaiI ACGT 3 cut(s) 37, 2112, 2225
TaqI TCGA 3 cut(s) 1297, 2186, 2611
TatI WGTACW 1 cut(s) 2204
TfiI GAWTC 5 cut(s) 233, 584, 1677, 1824, 2423
TscAI CASTG 3 cut(s) 329, 918, 2413
TseFI GTSAC 5 cut(s) 471, 856, 1538, 1886, 2100
TseI GCWGC 4 cut(s) 698, 893, 1154, 1561
Tsp45I GTSAC 5 cut(s) 471, 856, 1538, 1886, 2100
TspRI CASTG 3 cut(s) 329, 918, 2413
Van91I CCANNNNNTGG 1 cut(s) 1864
VpaK11BI GGWCC 3 cut(s) 173, 182, 394
XagI CCTNNNNNAGG 1 cut(s) 2582
XapI RAATTY 4 cut(s) 518, 524, 812, 2261
XceI RCATGY 5 cut(s) 444, 1195, 1966, 2541, 2636
XmaJI CCTAGG 1 cut(s) 1093
XmiI GTMKAC 4 cut(s) 572, 1488, 2280, 2486
XmnI GAANNNNTTC 1 cut(s) 1511
XspI CTAG 5 cut(s) 626, 867, 902, 1094, 2379
Zsp2I ATGCAT 2 cut(s) 837, 2638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.