Rh4BG242600

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0012544
Physical Location & Seq
Reverse (-)
40860285 .. 40863091
2807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG242600.1

Sequence Viewer

Length: 2670 bp
ATGCTCCTAAGAAAACAAGGAATACGTCATTTAGACGGATTCTGTAGCATTTACAATTTTTCTAAGAGGTTTTCATCCTCGCCAAATGGGCCTTCTTCTGGACTTGATGGGAAAGTTGATTCCTCTGACGGTGGGGTTAATTGTGATAACCCAAGGAAAGTGGAGGGTGTGTGCCAAGTCCTGGAGGGTGGTCCTTGGGGACCTGCCCTTGAGAATGTTCTATCTGCGCTTGACGAAAAACCTCAGTCCGACTTAGTTATTGGAGTCTTAAGGAGGCTGAAAGATGTCGGTCAAGCAATTAATTATTTTCGGTGGTGTGAGAAACAAACTGAGCAAGCACACTGTCCAGAAGTGTACAATTCACTTCTCCTGGTCATGGCCAGGAGTAGGAATTTTAATAGCTTGGAACAAGTTCTGGAAGAAATGAGTATTGCAGGAATTAGTCCATCAAATAATGCATGTGTTGAGTTAGTTGTGAGCTGTGTCAGGTCACAGAAACTGGGAGAAGCTTTTAATATACTGCAAACCATGAGGAAGTTCAAATTCCGCCCAGCATTTTCAGCCTATTCAACTCTGATTGGAGCTCTGTCGACAATTCCTGAACCTGATCGAATGCTGACTCTCTTTCATCAAATGCAGGAGCTAGGTTATGAAGTCACAGTGCATTTATTTACAACTCTTATTCGTGTATTTGCCAAGGAAGGTCGAGTTGACGCTGCCCTCTCCTTGTTGGATGAGATGAAGAGCAACTTTTGTGATGCAGATATTGTTCTTTATAATGTATGTATAGACTGCTTTGGCAAGATGGGTAAGGTGGATATGGCCTGGAAATTCTTTCATGAAATGAGAATGAACGGCTTGATGCCTGATGATGTGACATATACTAGCATGATAGGTGTTCTCTGCAAAGCTGAGAAACTAGACGAAGCTGTTAAGGTGTTTGAGGAGATGGATGCCAACAGGAAAGTACCTTGTGTGTATGCTTATAACACCATGATCATGGGCTATGGTTCTGCTGGAAAGTTTGATGAAGCATACAGCTTATTTGAGAGGCAAAAAAGAAAGGGGTGCATTCCGAGTGTAATTGCATATAATTGTGTTCTCACTTGCCTGGGGAAGAAGGGGAGATCTGAAGAAGCGTTGAGGGTTTTTAATGAGATGAAGACAGATGCAGCACCCAATATTTCAACATATAACATTTTAATAGACATGCTTTGCAAGGCAGGTAATGTTGAGGCTGCTCTGCGGGTTCAGGATGATATGAGAGAAGCTGGCTTATATCCTAATGTGATGACTGTAAACATAATGATTGATCGACTCTGTAAAGCACAGAAACTTGATGAAGCATGTTCTATATATGAAGGAATGGATCATAAAGTTTGCATGCCAGATGCAGTCACATTTTGTTCTCTTATAGATGGCTTGGGAAGACGTGGAAGAGTGAATGATGCCTATAGACTTCATGAAAAAATGCTAGATTCTGATCAGATTCCAAATGCTATAGCATACACATCTCTCATCAGGAACTTCTTCAAGTGTGGCAGGAAGGAGGATGGTCACAAAATATACAAAGAAATGGTGCGCAGGGGCTGCTCTCCTGACCTGATGCTCCTTAATACCTACATGGATTGTGTTTTCAAAGCTGGTGAAACAGACAAAGGTAGGTCTTTGTTTGAGGAGATTAAGGCTCAAGGATTCATCCCTGATGTGAGGAGCTATTCAATCTTAATTCATGGCCTTGTCAAAGCAGGTTTTGCACATGAAACCTATAAATTATTTCATGCAATGAAAGAACAGGGTTGTGAATTGGACACTCGAGCTTACAATGCTGTCATTGATGGATTCTGCAAATGTGGAAAGGTGAATAAAGCATATCAGCTGCTGGAGGAAATGAAAACTAAGGGTCAGCAACCCACTGTTGTAACCTATGGTTCTTTGATTGATGGGCTTGCCAAGATTGATAGACTTGATGAAGCGTACATGCTTTTTGAAGAAGCAAAGTCGAAAGGAGTAGAATTGAATGTAGTTGTTTATAGTAGTCTTGTCGATGGATTTGGGAAGGTGGGGAGAATTGATGAAGCATATCTAATCATGGAAGAGTTGATGCAAAAAGGTTTGACACCTAACGTGTACACATATAATTCCTTGCTCGATGCACTTGTGAAAGCTGGCGAAATTGATGAAGCCATTTTATGCTTTCAATCCATGAAGGATTTGAACTGTACACCCAACAGTTTTACTTATAATATTCTCATAAATGGTCTTTGTCGAGTTAGAAAATTCAACAAGGCTTTTGTATACTGGCAAGAAATGAAGAAACAGGGATTAAATCCCAACACAGTCACCTACTGTACCATGATCTCTGGACTTGCAAAAGCTGGGAACATAAGGGATGCTGCTGGGCTTTTTGAAAGGTTTAAGGCTAGTGGTGGTATTCTAGATTCTGCTTGTTATAATGCTATGATAGAAGGTTTAAGCAGTGGAGATAAGGCAATGGAAGCATATGCACTTTTTGAGGAAACTCGGCGGAAAGGCTGTAGTATTAATACCAAGACATGTGTTGTTTTATTGGACGCACTGCATAAGGCTGAATGCCTTGAACAGGCAGCAATAGTGGGTGCTGTACTGAGGGAAACTGCAAAGTCTCAGCATGCATCAAGATCATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

889

Amino Acids

99.34

Weight (kDa)

6.55

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 92 - 249 2.5e-08 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 138 - 244 7.9e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 138 - 196 3.4e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 208 - 269 2.3e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 216 - 248 2.6e-06 PPR repeat
PPR PF01535 224 - 249 8e-06 PPR repeat
PPR_long PF17177 239 - 376 2.8e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 255 - 303 3e-16 PPR repeat family
PPR PF01535 257 - 287 1.3e-06 PPR repeat
TPR_24 PF23276 259 - 350 3.2e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 279 - 337 3.3e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 285 - 317 3.9e-12 PPR repeat
PPR_2 PF13041 292 - 318 6.4e-06 PPR repeat family
PPR PF01535 292 - 318 7.8e-08 PPR repeat
PPR_2 PF13041 324 - 361 3.1e-08 PPR repeat family
PPR_3 PF13812 326 - 373 5.7e-06 Pentatricopeptide repeat domain
PPR PF01535 328 - 357 4.2e-07 PPR repeat
PPR_3 PF13812 348 - 405 1.6e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 355 - 388 3.8e-07 PPR repeat
PPR_2 PF13041 359 - 389 1.3e-07 PPR repeat family
PPR PF01535 363 - 389 3.3e-06 PPR repeat
TPR_24 PF23276 367 - 489 1.4e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 368 - 517 4.4e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 383 - 438 1.4e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 393 - 442 4.2e-16 PPR repeat family
PPR_1 PF12854 393 - 422 1.4e-09 PPR repeat
PPR PF01535 396 - 426 1.2e-06 PPR repeat
PPR_1 PF12854 425 - 456 7.3e-07 PPR repeat
PPR_2 PF13041 428 - 476 3.7e-12 PPR repeat family
PPR_1 PF12854 461 - 491 1.6e-09 PPR repeat
PPR_2 PF13041 470 - 512 5.4e-07 PPR repeat family
PPR_2 PF13041 498 - 534 4.7e-08 PPR repeat family
PPR_long PF17177 518 - 642 3.4e-09 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 533 - 582 6e-09 PPR repeat family
PPR_2 PF13041 576 - 616 9.9e-08 PPR repeat family
PPR_3 PF13812 591 - 648 1.9e-09 Pentatricopeptide repeat domain
TPR_24 PF23276 593 - 694 3.9e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 600 - 632 1.1e-12 PPR repeat
PPR_2 PF13041 604 - 652 1.7e-17 PPR repeat family
PPR PF01535 607 - 635 2.4e-08 PPR repeat
PPR_1 PF12854 634 - 665 3.2e-08 PPR repeat
PPR_2 PF13041 640 - 687 1e-10 PPR repeat family
PPR PF01535 641 - 671 5.3e-06 PPR repeat
PPR_long PF17177 653 - 734 3.5e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 662 - 722 5.3e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 669 - 700 4.9e-07 PPR repeat
PPR_2 PF13041 674 - 722 2.6e-12 PPR repeat family
TPR_24 PF23276 697 - 805 1.4e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 697 - 753 3.9e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 704 - 737 7.5e-08 PPR repeat
PPR PF01535 711 - 741 1e-06 PPR repeat
PPR_2 PF13041 712 - 757 6.9e-17 PPR repeat family
PPR_1 PF12854 740 - 772 7e-10 PPR repeat
PPR PF01535 746 - 776 4.4e-07 PPR repeat
PPR_2 PF13041 747 - 792 1.5e-16 PPR repeat family
PPR_1 PF12854 775 - 804 1.6e-08 PPR repeat
PPR_2 PF13041 814 - 862 7.8e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012544)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06920 AT3G06920 AT3G06920
fragaria_vesca FvH4_4g18230 FvH4_4g18230
malus_domestica MD08G1066700.v1.1
prunus_persica Prupe.1G135500_v2.0.a1
pyrus_communis pycom13g14370
rosa_chinensis RchiOBHm_Chr4g0422371
rosa_laevigata RLG00000007615
rosa_multiflora Rmu_sc0013245.1_g000002
rosa_roxburghii Rroxscaffold_5G00364490
rosa_rugosa Rorug04G0179500
rosa_samantha Rh4AG239000 Rh4AG239400 Rh4BG242600 Rh4CG254000 Rh4DG238100
rosa_wichuraiana Rw4G020660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 777, 987, 2244, 2454
Acc16I TGCGCA 1 cut(s) 1583
Acc36I ACCTGC 3 cut(s) 211, 1214, 1739
AccB7I CCANNNNNTGG 1 cut(s) 181
AccI GTMKAC 2 cut(s) 590, 2298
AciI CCGC 3 cut(s) 547, 1246, 2527
AclWI GGATC 1 cut(s) 1377
AcoI YGGCCR 1 cut(s) 378
AcsI RAATTY 4 cut(s) 391, 542, 830, 2279
AcuI CTGAAG 1 cut(s) 1152
AfaI GTAC 7 cut(s) 356, 969, 1979, 2132, 2224, 2353, 2625
AfiI CCNNNNNNNGG 5 cut(s) 98, 181, 376, 387, 2602
AflII CTTAAG 1 cut(s) 268
AflIII ACRYGT 2 cut(s) 2127, 2555
AjiI CACGTC 1 cut(s) 1433
AjnI CCWGG 5 cut(s) 180, 369, 380, 824, 1110
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
Alw21I GWGCWC 1 cut(s) 586
Alw26I GTCTC 1 cut(s) 2649
AlwI GGATC 1 cut(s) 1377
AlwNI CAGNNNCTG 3 cut(s) 499, 1590, 1882
Ama87I CYCGRG 1 cut(s) 1815
AoxI GGCC 4 cut(s) 89, 378, 822, 1735
ApeKI GCWGC 7 cut(s) 716, 1172, 1238, 1590, 1879, 2396, 2606
ApoI RAATTY 4 cut(s) 391, 542, 830, 2279
AseI ATTAAT 2 cut(s) 300, 2544
Asp700I GAANNNNTTC 2 cut(s) 411, 1529
AspLEI GCGC 2 cut(s) 229, 1584
AspS9I GGNCC 3 cut(s) 89, 191, 200
AsuHPI GGTGA 3 cut(s) 1658, 1873, 2335
AvaI CYCGRG 1 cut(s) 1815
AvaII GGWCC 2 cut(s) 191, 200
BalI TGGCCA 1 cut(s) 380
BanII GRGCYC 1 cut(s) 586
BbsI GAAGAC 2 cut(s) 1169, 1435
Bbv12I GWGCWC 1 cut(s) 586
BbvI GCAGC 7 cut(s) 703, 1184, 1225, 1577, 1866, 2383, 2618
BccI CCATC 9 cut(s) 101, 454, 799, 943, 1412, 1547, 1832, 1937, 2042
BceAI ACGGC 1 cut(s) 871
BciT130I CCWGG 5 cut(s) 182, 371, 382, 826, 1112
BclI TGATCA 2 cut(s) 996, 1483
BcoDI GTCTC 1 cut(s) 2649
BfaI CTAG 6 cut(s) 644, 885, 920, 1475, 2424, 2438
BfmI CTRYAG 4 cut(s) 43, 1453, 1500, 2536
BfrI CTTAAG 1 cut(s) 268
BfuAI ACCTGC 3 cut(s) 211, 1214, 1739
BglI GCCNNNNNGGC 1 cut(s) 88
BglII AGATCT 1 cut(s) 1127
BisI GCNGC 7 cut(s) 717, 1173, 1239, 1591, 1880, 2397, 2607
BlsI GCNGC 7 cut(s) 718, 1174, 1240, 1592, 1881, 2398, 2608
Bme1390I CCNGG 5 cut(s) 182, 371, 382, 826, 1112
Bme18I GGWCC 2 cut(s) 191, 200
BmeT110I CYCGRG 1 cut(s) 1815
BmgBI CACGTC 1 cut(s) 1433
BmgT120I GGNCC 3 cut(s) 89, 191, 200
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 5 cut(s) 182, 371, 382, 826, 1112
BmrI ACTGGG 1 cut(s) 509
BmuI ACTGGG 1 cut(s) 509
BpiI GAAGAC 2 cut(s) 1169, 1435
BpmI CTGGAG 2 cut(s) 203, 1904
BpuEI CTTGAG 2 cut(s) 230, 1674
BsaBI GATNNNNATC 1 cut(s) 1482
BsaJI CCNNGG 4 cut(s) 152, 194, 696, 1111
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 5 cut(s) 98, 181, 376, 387, 2602
Bse1I ACTGG 2 cut(s) 504, 2306
Bse3DI GCAATG 2 cut(s) 1791, 2499
Bse8I GATNNNNATC 1 cut(s) 1482
BseBI CCWGG 5 cut(s) 182, 371, 382, 826, 1112
BseDI CCNNGG 4 cut(s) 152, 194, 696, 1111
BseGI GGATG 7 cut(s) 74, 739, 958, 1261, 1558, 1698, 2398
BseJI GATNNNNATC 1 cut(s) 1482
BseLI CCNNNNNNNGG 5 cut(s) 98, 181, 376, 387, 2602
BseMI GCAATG 2 cut(s) 1791, 2499
BseMII CTCAG 5 cut(s) 257, 321, 903, 2618, 2660
BseNI ACTGG 2 cut(s) 504, 2306
BseRI GAGGAG 3 cut(s) 959, 1691, 1726
BseXI GCAGC 7 cut(s) 703, 1184, 1225, 1577, 1866, 2383, 2618
BseYI CCCAGC 3 cut(s) 550, 2378, 2399
BshFI GGCC 4 cut(s) 91, 380, 824, 1737
BsiHKAI GWGCWC 1 cut(s) 586
BsiHKCI CYCGRG 1 cut(s) 1815
BslFI GGGAC 1 cut(s) 213
BslI CCNNNNNNNGG 5 cut(s) 98, 181, 376, 387, 2602
BsmAI GTCTC 1 cut(s) 2649
BsmFI GGGAC 1 cut(s) 213
BsmI GAATGC 3 cut(s) 618, 1071, 2597
BsnI GGCC 4 cut(s) 91, 380, 824, 1737
BsoBI CYCGRG 1 cut(s) 1815
Bsp1286I GDGCHC 1 cut(s) 586
Bsp1407I TGTACA 3 cut(s) 354, 2130, 2222
Bsp143I GATC 8 cut(s) 607, 996, 1127, 1312, 1369, 1483, 2358, 2660
BspACI CCGC 3 cut(s) 547, 1246, 2527
BspANI GGCC 4 cut(s) 91, 380, 824, 1737
BspCNI CTCAG 5 cut(s) 256, 322, 904, 2619, 2659
BspHI TCATGA 2 cut(s) 838, 1462
BspLI GGNNCC 1 cut(s) 201
BspMI ACCTGC 3 cut(s) 211, 1214, 1739
BspPI GGATC 1 cut(s) 1377
BspQI GCTCTTC 1 cut(s) 737
BspTI CTTAAG 1 cut(s) 268
BsrDI GCAATG 2 cut(s) 1791, 2499
BsrGI TGTACA 3 cut(s) 354, 2130, 2222
BsrI ACTGG 2 cut(s) 504, 2306
BssECI CCNNGG 4 cut(s) 152, 194, 696, 1111
BssMI GATC 8 cut(s) 607, 996, 1127, 1312, 1369, 1483, 2358, 2660
BssNAI GTATAC 1 cut(s) 2299
BssT1I CCWWGG 3 cut(s) 152, 194, 696
Bst1107I GTATAC 1 cut(s) 2299
Bst2UI CCWGG 5 cut(s) 182, 371, 382, 826, 1112
Bst4CI ACNGT 9 cut(s) 131, 344, 661, 1297, 1918, 2222, 2234, 2341, 2351
Bst6I CTCTTC 3 cut(s) 737, 1432, 2091
BstAFI CTTAAG 1 cut(s) 268
BstAPI GCANNNNNTGC 2 cut(s) 1590, 1754
BstAUI TGTACA 3 cut(s) 354, 2130, 2222
BstC8I GCNNGC 6 cut(s) 336, 1273, 1385, 1950, 2170, 2652
BstDEI CTNAG 9 cut(s) 8, 63, 243, 253, 330, 912, 1899, 2627, 2646
BstENI CCTNNNNNAGG 1 cut(s) 2600
BstF5I GGATG 7 cut(s) 74, 739, 958, 1261, 1558, 1698, 2398
BstHHI GCGC 2 cut(s) 229, 1584
BstKTI GATC 8 cut(s) 610, 999, 1130, 1315, 1372, 1486, 2361, 2663
BstMAI GTCTC 1 cut(s) 2649
BstMBI GATC 8 cut(s) 607, 996, 1127, 1312, 1369, 1483, 2358, 2660
BstMWI GCNNNNNNNGC 6 cut(s) 88, 560, 1590, 1754, 1826, 2498
BstNI CCWGG 5 cut(s) 182, 371, 382, 826, 1112
BstNSI RCATGY 7 cut(s) 462, 1213, 1350, 1387, 1984, 2559, 2654
BstSCI CCNGG 5 cut(s) 180, 369, 380, 824, 1110
BstSFI CTRYAG 4 cut(s) 43, 1453, 1500, 2536
BstV1I GCAGC 7 cut(s) 703, 1184, 1225, 1577, 1866, 2383, 2618
BstV2I GAAGAC 2 cut(s) 1169, 1435
BstX2I RGATCY 1 cut(s) 1127
BstXI CCANNNNNNTGG 1 cut(s) 1000
BstYI RGATCY 1 cut(s) 1127
BstZ17I GTATAC 1 cut(s) 2299
BsuRI GGCC 4 cut(s) 91, 380, 824, 1737
BtrI CACGTC 1 cut(s) 1433
BtsCI GGATG 7 cut(s) 74, 739, 958, 1261, 1558, 1698, 2398
BtsI GCAGTG 2 cut(s) 2485, 2576
BtsIMutI CAGTG 5 cut(s) 340, 666, 1914, 2485, 2576
BveI ACCTGC 3 cut(s) 211, 1214, 1739
Cac8I GCNNGC 6 cut(s) 336, 1273, 1385, 1950, 2170, 2652
CaiI CAGNNNCTG 3 cut(s) 499, 1590, 1882
CciI TCATGA 2 cut(s) 838, 1462
CfoI GCGC 2 cut(s) 229, 1584
Cfr13I GGNCC 3 cut(s) 89, 191, 200
CseI GACGC 2 cut(s) 722, 2582
Csp6I GTAC 7 cut(s) 355, 968, 1978, 2131, 2223, 2352, 2624
CspCI CAANNNNNGTGG 2 cut(s) 141, 176
CviQI GTAC 7 cut(s) 355, 968, 1978, 2131, 2223, 2352, 2624
DdeI CTNAG 9 cut(s) 8, 63, 243, 253, 330, 912, 1899, 2627, 2646
DpnI GATC 8 cut(s) 609, 998, 1129, 1314, 1371, 1485, 2360, 2662
DpnII GATC 8 cut(s) 607, 996, 1127, 1312, 1369, 1483, 2358, 2660
EaeI YGGCCR 1 cut(s) 378
Eam1104I CTCTTC 3 cut(s) 737, 1432, 2091
EarI CTCTTC 3 cut(s) 737, 1432, 2091
EciI GGCGGA 2 cut(s) 536, 2542
Ecl136II GAGCTC 1 cut(s) 584
Eco130I CCWWGG 3 cut(s) 152, 194, 696
Eco24I GRGCYC 1 cut(s) 586
Eco47I GGWCC 2 cut(s) 191, 200
Eco53kI GAGCTC 1 cut(s) 584
Eco57I CTGAAG 1 cut(s) 1152
Eco88I CYCGRG 1 cut(s) 1815
EcoICRI GAGCTC 1 cut(s) 584
EcoNI CCTNNNNNAGG 1 cut(s) 2600
EcoO109I RGGNCCY 1 cut(s) 200
EcoRII CCWGG 5 cut(s) 180, 369, 380, 824, 1110
EcoT14I CCWWGG 3 cut(s) 152, 194, 696
EcoT22I ATGCAT 2 cut(s) 460, 2656
EcoT38I GRGCYC 1 cut(s) 586
ErhI CCWWGG 3 cut(s) 152, 194, 696
FalI AAGNNNNNCTT 2 cut(s) 734, 766
FaqI GGGAC 1 cut(s) 213
FauI CCCGC 1 cut(s) 1239
FauNDI CATATG 1 cut(s) 2503
FbaI TGATCA 2 cut(s) 996, 1483
FblI GTMKAC 2 cut(s) 590, 2298
Fnu4HI GCNGC 7 cut(s) 717, 1173, 1239, 1591, 1880, 2397, 2607
FokI GGATG 7 cut(s) 61, 746, 965, 1268, 1565, 1685, 2405
FriOI GRGCYC 1 cut(s) 586
Fsp4HI GCNGC 7 cut(s) 717, 1173, 1239, 1591, 1880, 2397, 2607
FspBI CTAG 6 cut(s) 644, 885, 920, 1475, 2424, 2438
FspI TGCGCA 1 cut(s) 1583
GlaI GCGC 2 cut(s) 228, 1583
GluI GCNGC 7 cut(s) 717, 1173, 1239, 1591, 1880, 2397, 2607
GsaI CCCAGC 3 cut(s) 554, 2382, 2403
GsuI CTGGAG 2 cut(s) 203, 1904
HaeIII GGCC 4 cut(s) 91, 380, 824, 1737
HgaI GACGC 2 cut(s) 722, 2582
HhaI GCGC 2 cut(s) 229, 1584
Hin6I GCGC 2 cut(s) 227, 1582
HinP1I GCGC 2 cut(s) 227, 1582
HincII GTYRAC 2 cut(s) 591, 712
HindII GTYRAC 2 cut(s) 591, 712
HindIII AAGCTT 1 cut(s) 507
HphI GGTGA 3 cut(s) 1658, 1873, 2335
Hpy166II GTNNAC 8 cut(s) 355, 591, 712, 1300, 2131, 2133, 2225, 2299
Hpy188I TCNGA 7 cut(s) 127, 250, 576, 1077, 1132, 1483, 1488
Hpy8I GTNNAC 8 cut(s) 355, 591, 712, 1300, 2131, 2133, 2225, 2299
HpyAV CCTTC 8 cut(s) 102, 695, 1114, 1355, 1540, 2053, 2203, 2462
HpyCH4III ACNGT 9 cut(s) 131, 344, 661, 1297, 1918, 2222, 2234, 2341, 2351
HpyCH4IV ACGT 3 cut(s) 25, 1432, 2127
HpyF10VI GCNNNNNNNGC 6 cut(s) 88, 560, 1590, 1754, 1826, 2498
HpyF3I CTNAG 9 cut(s) 8, 63, 243, 253, 330, 912, 1899, 2627, 2646
HpySE526I ACGT 3 cut(s) 25, 1432, 2127
HspAI GCGC 2 cut(s) 227, 1582
Ksp22I TGATCA 2 cut(s) 996, 1483
Kzo9I GATC 8 cut(s) 607, 996, 1127, 1312, 1369, 1483, 2358, 2660
LguI GCTCTTC 1 cut(s) 737
LmnI GCTCC 5 cut(s) 9, 581, 640, 1614, 1713
Lsp1109I GCAGC 7 cut(s) 703, 1184, 1225, 1577, 1866, 2383, 2618
MaeI CTAG 6 cut(s) 644, 885, 920, 1475, 2424, 2438
MaeII ACGT 3 cut(s) 25, 1432, 2127
MaeIII GTNAC 7 cut(s) 489, 655, 874, 1396, 1556, 1921, 2341
MalI GATC 8 cut(s) 609, 998, 1129, 1314, 1371, 1485, 2360, 2662
MboI GATC 8 cut(s) 607, 996, 1127, 1312, 1369, 1483, 2358, 2660
MflI RGATCY 1 cut(s) 1127
MhlI GDGCHC 1 cut(s) 586
MlsI TGGCCA 1 cut(s) 380
MluNI TGGCCA 1 cut(s) 380
MlyI GAGTC 3 cut(s) 273, 613, 1311
MmeI TCCRAC 2 cut(s) 273, 711
Mox20I TGGCCA 1 cut(s) 380
Mph1103I ATGCAT 2 cut(s) 460, 2656
MroXI GAANNNNTTC 2 cut(s) 411, 1529
MscI TGGCCA 1 cut(s) 380
MslI CAYNNNNRTG 1 cut(s) 998
Msp20I TGGCCA 1 cut(s) 380
MspA1I CMGCKG 1 cut(s) 1879
MspCI CTTAAG 1 cut(s) 268
MspR9I CCNGG 5 cut(s) 182, 371, 382, 826, 1112
Mva1269I GAATGC 3 cut(s) 618, 1071, 2597
MvaI CCWGG 5 cut(s) 182, 371, 382, 826, 1112
MwoI GCNNNNNNNGC 6 cut(s) 88, 560, 1590, 1754, 1826, 2498
NdeI CATATG 1 cut(s) 2503
NdeII GATC 8 cut(s) 607, 996, 1127, 1312, 1369, 1483, 2358, 2660
NlaIV GGNNCC 1 cut(s) 201
NmeAIII GCCGAG 1 cut(s) 2503
NmuCI GTSAC 6 cut(s) 489, 655, 874, 1396, 1556, 2341
NsbI TGCGCA 1 cut(s) 1583
NsiI ATGCAT 2 cut(s) 460, 2656
NspI RCATGY 7 cut(s) 462, 1213, 1350, 1387, 1984, 2559, 2654
PaeI GCATGC 2 cut(s) 1387, 2654
PaeR7I CTCGAG 1 cut(s) 1815
PagI TCATGA 2 cut(s) 838, 1462
PciI ACATGT 1 cut(s) 2555
PciSI GCTCTTC 1 cut(s) 737
PctI GAATGC 3 cut(s) 618, 1071, 2597
PdmI GAANNNNTTC 2 cut(s) 411, 1529
PfeI GAWTC 7 cut(s) 39, 119, 1478, 1489, 1695, 1842, 2441
PflMI CCANNNNNTGG 1 cut(s) 181
PfoI TCCNGGA 1 cut(s) 180
PkrI GCNGC 7 cut(s) 718, 1174, 1240, 1592, 1881, 2398, 2608
PleI GAGTC 3 cut(s) 272, 613, 1311
PpsI GAGTC 3 cut(s) 272, 613, 1311
PpuMI RGGWCCY 1 cut(s) 200
PscI ACATGT 1 cut(s) 2555
PshBI ATTAAT 2 cut(s) 300, 2544
PsiI TTATAA 4 cut(s) 777, 987, 2244, 2454
Psp124BI GAGCTC 1 cut(s) 586
Psp5II RGGWCCY 1 cut(s) 200
Psp6I CCWGG 5 cut(s) 180, 369, 380, 824, 1110
PspFI CCCAGC 3 cut(s) 550, 2378, 2399
PspGI CCWGG 5 cut(s) 180, 369, 380, 824, 1110
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 3 cut(s) 89, 191, 200
PspPPI RGGWCCY 1 cut(s) 200
PspXI VCTCGAGB 1 cut(s) 1815
PstNI CAGNNNCTG 3 cut(s) 499, 1590, 1882
PsuI RGATCY 1 cut(s) 1127
PvuII CAGCTG 1 cut(s) 1879
RsaI GTAC 7 cut(s) 356, 969, 1979, 2132, 2224, 2353, 2625
RsaNI GTAC 7 cut(s) 355, 968, 1978, 2131, 2223, 2352, 2624
RseI CAYNNNNRTG 1 cut(s) 998
SacI GAGCTC 1 cut(s) 586
SalI GTCGAC 1 cut(s) 589
SapI GCTCTTC 1 cut(s) 737
SatI GCNGC 7 cut(s) 717, 1173, 1239, 1591, 1880, 2397, 2607
Sau3AI GATC 8 cut(s) 607, 996, 1127, 1312, 1369, 1483, 2358, 2660
Sau96I GGNCC 3 cut(s) 89, 191, 200
SchI GAGTC 3 cut(s) 273, 613, 1311
ScrFI CCNGG 5 cut(s) 182, 371, 382, 826, 1112
SduI GDGCHC 1 cut(s) 586
SfcI CTRYAG 4 cut(s) 43, 1453, 1500, 2536
Sfr274I CTCGAG 1 cut(s) 1815
SinI GGWCC 2 cut(s) 191, 200
SlaI CTCGAG 1 cut(s) 1815
SmiMI CAYNNNNRTG 1 cut(s) 998
SmlI CTYRAG 4 cut(s) 209, 268, 1689, 1815
SmoI CTYRAG 4 cut(s) 209, 268, 1689, 1815
SphI GCATGC 2 cut(s) 1387, 2654
SsiI CCGC 3 cut(s) 547, 1246, 2527
SspI AATATT 2 cut(s) 1183, 2248
SspMI CTAG 6 cut(s) 644, 885, 920, 1475, 2424, 2438
SstI GAGCTC 1 cut(s) 586
StyD4I CCNGG 5 cut(s) 180, 369, 380, 824, 1110
StyI CCWWGG 3 cut(s) 152, 194, 696
TaaI ACNGT 9 cut(s) 131, 344, 661, 1297, 1918, 2222, 2234, 2341, 2351
TaiI ACGT 3 cut(s) 28, 1435, 2130
TaqI TCGA 9 cut(s) 590, 610, 706, 1315, 1816, 2003, 2046, 2151, 2269
TaqII GACCGA 1 cut(s) 278
TatI WGTACW 4 cut(s) 354, 2130, 2222, 2623
TfiI GAWTC 7 cut(s) 39, 119, 1478, 1489, 1695, 1842, 2441
TscAI CASTG 5 cut(s) 347, 666, 1921, 2485, 2583
TseFI GTSAC 6 cut(s) 489, 655, 874, 1396, 1556, 2341
TseI GCWGC 7 cut(s) 716, 1172, 1238, 1590, 1879, 2396, 2606
Tsp45I GTSAC 6 cut(s) 489, 655, 874, 1396, 1556, 2341
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 5 cut(s) 347, 666, 1921, 2485, 2583
Van91I CCANNNNNTGG 1 cut(s) 181
Vha464I CTTAAG 1 cut(s) 268
VpaK11BI GGWCC 2 cut(s) 191, 200
VspI ATTAAT 2 cut(s) 300, 2544
XagI CCTNNNNNAGG 1 cut(s) 2600
XapI RAATTY 4 cut(s) 391, 542, 830, 2279
XbaI TCTAGA 1 cut(s) 2437
XceI RCATGY 7 cut(s) 462, 1213, 1350, 1387, 1984, 2559, 2654
XhoI CTCGAG 1 cut(s) 1815
XmiI GTMKAC 2 cut(s) 590, 2298
XmnI GAANNNNTTC 2 cut(s) 411, 1529
XspI CTAG 6 cut(s) 644, 885, 920, 1475, 2424, 2438
Zsp2I ATGCAT 2 cut(s) 460, 2656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.