RLG00000007615

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012544
Physical Location & Seq
Forward (+)
19039304 .. 19043784
4481 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007615

Sequence Viewer

Length: 2694 bp
ATGCTCCTAAGAAAACAAGGAATACGTCATTTAGACGGATTCTGTAGCATTTACAATTTTTCTAAGAGCTTTTCATCCTCACCAAATGGGCCTTCTTCTGGACTTGATGGGAAAGTTGATTCCTCTGACGGTCGGGTTAATTGTGATAACTCGAGGAAAGTGGAGGGTGTGAGACAAGTGGTGGATGGTGTGTGCCAAGTCCTGAAGGGTGGTCCTTGGGGACCTGCCCTTGAGAATGCTCTATCTGCGCTTGACGAAAAACCTCAATCCGACTTAGTTATTGGAGTCTTAAGGAGGCTGAAAGATGTCGGTCAAGCAATTAATTATTTTCGGTGGTGTGAGAAACAAACTGAGCAAGCACAGTGTCCAGAAGCGTACAATTCACTTCTCCTGGTCATGGCCAGGAGTAGGAATTTTAATAGCTTGGAACAAGTTCTGGAGGAAATGAGTATTGCAGGAATTAGTCCATCAAATAATGCATGTGTCGAGTTAGTTGTGAGCTGTGTCAGGTCACAGAAACTGAGAGAAGCTTTTAATCTACTGCAAACCATGAGGAAGTTCAAATTCCGCCCAGCATTTTCAGCCTATTCAACTCTGATTGGAGCTCTGTCGACAATTCCTGAACCTGATCGAATGCTGACTCTCTTTCATCAAATGCAGGAGCTAGGCTATGAAGTCACCGTGCATTTATTTACAACTCTTATTCGTGTATTTGCCAAGGAAGGCCGAGTTGACGCTGCCCTCTCCTTGTTGGATGAGATGAAGAGCAACTTTTGTGATGCAGACATTGTTCTTTATAATGTGTGTATAGACTGCTTTGGCAAGGTGGGTAAGGTGGATATGGCCTGGAAATTCTTTCATGAAATGAGAATGAACGGCTTGATGCCTGATGATGTGACATATACTAGCATGATAGGTGTTCTCTGCAAAGCTGAGAAACTAGACGAAGCTGTTAAGGTGTTTGAGGAAATGGATGTCAACAGGAAAGTACCTTGTGTGTATGCTTATAACACCATGATCATGGGCTATGGCTCTGCTGGAAAGTTCGATGAAGCATACAGCTTATTTGAGAGGCAAAAAAGAAAGGGGTGCATTCCGAGTGTAATTGCATATAATTGTGTTCTCACTTGCCTGGGGAAGAAGGGGAGATCTGAAGAAGCGTTGAGGGTTTTTAATGAGATGAAGACAGATGCAGCACCCAATATTTCAACATATAACATTTTAATAGACATGCTTTGCAGGGCAGGTAATGTTGAGGCCGCTCTGCAGGTTCAGGATGATATGAGAGAAGCTGGCTTATATCCTAATGTGATGACTGTAAACATAATGATTGATCGACTCTGTAAAGCACAGAAACTTGATGAAGCATGTTCTATATATGAAGGAATGGATCATAAAGTTTGCATGCCAGATGCAGTTACATTTTGTTCTCTTATAGATGGCTTGGGAAGACGTGGAAGAGTGAATGATGCCTATAGACTTTATGAAAAAATGCTAGATTCTGATCAGATTCCAAATGCTATAGCATACACATCTCTCATCAGGAACTTCTTCAAGTGTGGCAGGAAGGAGGATGGTCACAAAATATACAAAGAAATGGTGCGCAGGGGCTGCTCTCCTGACCTGATGCTCCTTAATACCTACATGGATTGTGTTTTCAAAGCTGGTGAAACAGACAAAGGTAGATCTTTGTTTGAGGAGATTAAGGCTCAAGGATTCATCCCTGATGTGAGGAGCTATTCAATATTAATTCATGGCCTTGTCAAAGCAGGTTTTGCACATGAAACCTATAAATTATTTCATGCAATGAAAGAACAGGGTTGTGAATTGGACACTCGAGCTTACAATGCCGTCATTGATGGATTCTGCAAATGTGGAAAGGTGAATAAAGCATATCAGCTGCTGGAGGAAATGAAAACTAAGGGTCAGCAACCCACTGTTGTAACTTATGGTTCTGTGATTGATGGGCTTGCCAAGATTGATAGACTTGATGAAGCGTACATGCTTTTTGAAGAAGCAAAGTCGTCGAAAGGAGTAGAATTAAATGTAGTTGTTTATAGTAGTCTTGTCGATGGATTTGGCAAGGTGGGGAGAATTGATGAAGCATATCTAATCATGGAAGAGTTGATGCAAAAAGGTTTGACACCTAACGTGTACACGTATAATTCCTTGCTCGATGCACTTGTGAAAGCTGGCGAAGTTGATGAAGCCATTCTATGCTTTCAATCCATGAAGGATTTGAACTGTACACCCAACAGTTTTACTTATAATATTCTCATAAATGGGCTTTGTCGAGTTAGAAAATTCAACAAGGCTTTTGTGTACTGGCAAGAAATGAAGAAACAGGGATTAAATCCCAACACAGTCACCTACTGTACCATGATCTCTGGACTTGCAAAAGCTGGGAACATAAGGGATGCTACTGGGCTTTTTGAGAGGTTTAAGGCTAGTGGTGGCATTCCAGATTCTGCTTGTTATAATGCTATGATAGAAGGTTTAAGCAGCGGAGATAAGGCAATGGAAGCATATGCACTTTTTGAGGAAACTCGGCGGAAAGGCTGTAGTATTAATACCAAGACATGTGTTGTTTTATTGGACTCACTGCACAAGGCTGAATGCCTTGAACAGGCAGCAATAGTGGGTGCTGTACTGAGGGAAACTGCAAAGTCTCAGCATGCATCAAGATCATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

898

Amino Acids

100.26

Weight (kDa)

6.83

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 99 - 256 1.8e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 145 - 203 7.2e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 215 - 275 4.9e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 223 - 255 2.6e-06 PPR repeat
PPR_2 PF13041 228 - 263 1e-05 PPR repeat family
PPR PF01535 231 - 256 8.1e-06 PPR repeat
PPR_long PF17177 240 - 361 1.7e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 262 - 310 2.4e-16 PPR repeat family
PPR PF01535 264 - 294 2.1e-06 PPR repeat
TPR_24 PF23276 266 - 357 1.4e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 286 - 344 7.1e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 292 - 324 3.9e-12 PPR repeat
PPR PF01535 299 - 325 8.4e-08 PPR repeat
PPR_2 PF13041 299 - 325 7.3e-06 PPR repeat family
PPR_2 PF13041 331 - 368 3.1e-08 PPR repeat family
PPR_3 PF13812 333 - 380 5.7e-06 Pentatricopeptide repeat domain
PPR PF01535 335 - 364 4.2e-07 PPR repeat
PPR_3 PF13812 355 - 411 2.1e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 362 - 395 3.8e-07 PPR repeat
PPR_2 PF13041 366 - 396 1.3e-07 PPR repeat family
PPR PF01535 370 - 396 3.4e-06 PPR repeat
TPR_24 PF23276 374 - 497 1.9e-07 Fungal tetratrico peptide repeats
PPR_long PF17177 378 - 524 5.6e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 390 - 445 5.5e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 400 - 429 1.1e-09 PPR repeat
PPR_2 PF13041 400 - 449 7e-16 PPR repeat family
PPR PF01535 403 - 433 1.6e-06 PPR repeat
PPR_1 PF12854 432 - 463 7.4e-07 PPR repeat
PPR_2 PF13041 435 - 483 3.7e-12 PPR repeat family
PPR_1 PF12854 468 - 498 1.1e-09 PPR repeat
PPR_2 PF13041 477 - 519 5.2e-08 PPR repeat family
PPR_long PF17177 488 - 605 1.2e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 505 - 541 4.8e-08 PPR repeat family
PPR_long PF17177 525 - 650 3.1e-09 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 540 - 589 6.1e-09 PPR repeat family
PPR_3 PF13812 598 - 654 5.4e-09 Pentatricopeptide repeat domain
TPR_24 PF23276 600 - 683 1.7e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 607 - 639 1.1e-12 PPR repeat
PPR_2 PF13041 611 - 659 6.5e-17 PPR repeat family
PPR PF01535 614 - 642 2.4e-08 PPR repeat
PPR_1 PF12854 641 - 672 2.5e-07 PPR repeat
PPR_2 PF13041 645 - 695 2.4e-09 PPR repeat family
PPR_long PF17177 660 - 754 1e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 672 - 730 2.7e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 677 - 708 5e-07 PPR repeat
PPR_2 PF13041 682 - 730 2.7e-12 PPR repeat family
PPR_3 PF13812 705 - 761 3e-12 Pentatricopeptide repeat domain
TPR_24 PF23276 705 - 813 2e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 712 - 745 2.5e-08 PPR repeat
PPR_2 PF13041 716 - 765 2.4e-19 PPR repeat family
PPR PF01535 719 - 749 6.6e-07 PPR repeat
PPR_1 PF12854 748 - 780 7e-10 PPR repeat
PPR_2 PF13041 751 - 800 1.2e-18 PPR repeat family
PPR PF01535 754 - 784 4.5e-07 PPR repeat
PPR_3 PF13812 777 - 831 2.9e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 783 - 812 8.3e-09 PPR repeat
PPR_2 PF13041 821 - 870 4.2e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012544)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06920 AT3G06920 AT3G06920
fragaria_vesca FvH4_4g18230 FvH4_4g18230
malus_domestica MD08G1066700.v1.1
prunus_persica Prupe.1G135500_v2.0.a1
pyrus_communis pycom13g14370
rosa_chinensis RchiOBHm_Chr4g0422371
rosa_laevigata RLG00000007615
rosa_multiflora Rmu_sc0013245.1_g000002
rosa_roxburghii Rroxscaffold_5G00364490
rosa_rugosa Rorug04G0179500
rosa_samantha Rh4AG239000 Rh4AG239400 Rh4BG242600 Rh4CG254000 Rh4DG238100
rosa_wichuraiana Rw4G020660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 798, 1008, 2268, 2478
Acc16I TGCGCA 1 cut(s) 1604
Acc36I ACCTGC 4 cut(s) 232, 1235, 1258, 1760
AccBSI CCGCTC 1 cut(s) 1262
AccI GTMKAC 1 cut(s) 611
AciI CCGC 4 cut(s) 568, 1260, 2505, 2551
AclWI GGATC 1 cut(s) 1398
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 4 cut(s) 412, 563, 851, 2303
AcuI CTGAAG 2 cut(s) 224, 1173
AfaI GTAC 8 cut(s) 377, 990, 2000, 2156, 2248, 2324, 2377, 2649
AfiI CCNNNNNNNGG 4 cut(s) 98, 397, 408, 2626
AflII CTTAAG 1 cut(s) 289
AflIII ACRYGT 3 cut(s) 2151, 2157, 2579
AjiI CACGTC 1 cut(s) 1454
AjnI CCWGG 4 cut(s) 390, 401, 845, 1131
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
Alw21I GWGCWC 1 cut(s) 607
Alw26I GTCTC 2 cut(s) 166, 2673
AlwI GGATC 1 cut(s) 1398
AlwNI CAGNNNCTG 4 cut(s) 520, 1611, 1903, 2468
Ama87I CYCGRG 2 cut(s) 151, 1836
AoxI GGCC 6 cut(s) 89, 399, 724, 843, 1257, 1756
ApeKI GCWGC 6 cut(s) 737, 1193, 1611, 1900, 2502, 2630
ApoI RAATTY 4 cut(s) 412, 563, 851, 2303
AseI ATTAAT 3 cut(s) 321, 1748, 2568
Asp700I GAANNNNTTC 3 cut(s) 432, 1550, 2211
AspLEI GCGC 2 cut(s) 250, 1605
AspS9I GGNCC 3 cut(s) 89, 212, 221
AsuHPI GGTGA 5 cut(s) 72, 670, 1679, 1894, 2359
AvaI CYCGRG 2 cut(s) 151, 1836
AvaII GGWCC 2 cut(s) 212, 221
BalI TGGCCA 1 cut(s) 401
BanII GRGCYC 1 cut(s) 607
BbsI GAAGAC 2 cut(s) 1190, 1456
Bbv12I GWGCWC 1 cut(s) 607
BbvI GCAGC 6 cut(s) 724, 1205, 1598, 1887, 2514, 2642
BccI CCATC 8 cut(s) 101, 179, 475, 1433, 1568, 1853, 1958, 2066
BceAI ACGGC 2 cut(s) 892, 1835
BcgI CGANNNNNNTGC 2 cut(s) 2007, 2041
BciT130I CCWGG 4 cut(s) 392, 403, 847, 1133
BclI TGATCA 2 cut(s) 1017, 1504
BcoDI GTCTC 2 cut(s) 166, 2673
BfaI CTAG 5 cut(s) 665, 906, 941, 1496, 2448
BfmI CTRYAG 5 cut(s) 43, 1265, 1474, 1521, 2560
BfrI CTTAAG 1 cut(s) 289
BfuAI ACCTGC 4 cut(s) 232, 1235, 1258, 1760
BglII AGATCT 2 cut(s) 1148, 1685
BisI GCNGC 7 cut(s) 738, 1194, 1260, 1612, 1901, 2503, 2631
BlsI GCNGC 7 cut(s) 739, 1195, 1261, 1613, 1902, 2504, 2632
Bme1390I CCNGG 4 cut(s) 392, 403, 847, 1133
Bme18I GGWCC 2 cut(s) 212, 221
BmeT110I CYCGRG 2 cut(s) 151, 1836
BmgBI CACGTC 1 cut(s) 1454
BmgT120I GGNCC 3 cut(s) 89, 212, 221
BmiI GGNNCC 1 cut(s) 222
BmrFI CCNGG 4 cut(s) 392, 403, 847, 1133
BmrI ACTGGG 1 cut(s) 2433
BmuI ACTGGG 1 cut(s) 2433
BpiI GAAGAC 2 cut(s) 1190, 1456
BpmI CTGGAG 2 cut(s) 458, 1925
BpuEI CTTGAG 2 cut(s) 251, 1695
BsaAI YACGTR 1 cut(s) 2160
BsaBI GATNNNNATC 1 cut(s) 1503
BsaJI CCNNGG 3 cut(s) 215, 717, 1132
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 397, 408, 2626
Bse1I ACTGG 2 cut(s) 2330, 2428
Bse3DI GCAATG 2 cut(s) 1812, 2523
Bse8I GATNNNNATC 1 cut(s) 1503
BseBI CCWGG 4 cut(s) 392, 403, 847, 1133
BseDI CCNNGG 3 cut(s) 215, 717, 1132
BseGI GGATG 8 cut(s) 74, 190, 760, 979, 1282, 1579, 1719, 2422
BseJI GATNNNNATC 1 cut(s) 1503
BseLI CCNNNNNNNGG 4 cut(s) 98, 397, 408, 2626
BseMI GCAATG 2 cut(s) 1812, 2523
BseMII CTCAG 5 cut(s) 342, 512, 924, 2642, 2684
BseNI ACTGG 2 cut(s) 2330, 2428
BseRI GAGGAG 2 cut(s) 1712, 1747
BseXI GCAGC 6 cut(s) 724, 1205, 1598, 1887, 2514, 2642
BseYI CCCAGC 2 cut(s) 571, 2402
BsgI GTGCAG 1 cut(s) 2588
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 6 cut(s) 91, 401, 726, 845, 1259, 1758
BsiEI CGRYCG 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 607
BsiHKCI CYCGRG 2 cut(s) 151, 1836
BslFI GGGAC 1 cut(s) 234
BslI CCNNNNNNNGG 4 cut(s) 98, 397, 408, 2626
BsmAI GTCTC 2 cut(s) 166, 2673
BsmFI GGGAC 1 cut(s) 234
BsmI GAATGC 5 cut(s) 241, 639, 1092, 2457, 2621
BsnI GGCC 6 cut(s) 91, 401, 726, 845, 1259, 1758
BsoBI CYCGRG 2 cut(s) 151, 1836
Bsp1286I GDGCHC 1 cut(s) 607
Bsp1407I TGTACA 2 cut(s) 2154, 2246
Bsp143I GATC 9 cut(s) 628, 1017, 1148, 1333, 1390, 1504, 1685, 2382, 2684
BspACI CCGC 4 cut(s) 568, 1260, 2505, 2551
BspANI GGCC 6 cut(s) 91, 401, 726, 845, 1259, 1758
BspCNI CTCAG 5 cut(s) 343, 513, 925, 2643, 2683
BspHI TCATGA 1 cut(s) 859
BspLI GGNNCC 1 cut(s) 222
BspMAI CTGCAG 1 cut(s) 1269
BspMI ACCTGC 4 cut(s) 232, 1235, 1258, 1760
BspPI GGATC 1 cut(s) 1398
BspQI GCTCTTC 1 cut(s) 758
BspTI CTTAAG 1 cut(s) 289
BsrBI CCGCTC 1 cut(s) 1262
BsrDI GCAATG 2 cut(s) 1812, 2523
BsrGI TGTACA 2 cut(s) 2154, 2246
BsrI ACTGG 2 cut(s) 2330, 2428
BssECI CCNNGG 3 cut(s) 215, 717, 1132
BssMI GATC 9 cut(s) 628, 1017, 1148, 1333, 1390, 1504, 1685, 2382, 2684
BssT1I CCWWGG 2 cut(s) 215, 717
Bst2UI CCWGG 4 cut(s) 392, 403, 847, 1133
Bst4CI ACNGT 9 cut(s) 131, 363, 682, 1318, 1939, 2246, 2258, 2365, 2375
Bst6I CTCTTC 3 cut(s) 758, 1453, 2115
BstAFI CTTAAG 1 cut(s) 289
BstAPI GCANNNNNTGC 2 cut(s) 1611, 1775
BstAUI TGTACA 2 cut(s) 2154, 2246
BstBAI YACGTR 1 cut(s) 2160
BstC8I GCNNGC 6 cut(s) 357, 1294, 1406, 1971, 2194, 2676
BstDEI CTNAG 9 cut(s) 8, 63, 274, 351, 521, 933, 1920, 2651, 2670
BstENI CCTNNNNNAGG 1 cut(s) 2624
BstF5I GGATG 8 cut(s) 74, 190, 760, 979, 1282, 1579, 1719, 2422
BstHHI GCGC 2 cut(s) 250, 1605
BstKTI GATC 9 cut(s) 631, 1020, 1151, 1336, 1393, 1507, 1688, 2385, 2687
BstMAI GTCTC 2 cut(s) 166, 2673
BstMBI GATC 9 cut(s) 628, 1017, 1148, 1333, 1390, 1504, 1685, 2382, 2684
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 6 cut(s) 245, 581, 1611, 1775, 1847, 2522
BstNI CCWGG 4 cut(s) 392, 403, 847, 1133
BstNSI RCATGY 7 cut(s) 483, 1234, 1371, 1408, 2005, 2583, 2678
BstSCI CCNGG 4 cut(s) 390, 401, 845, 1131
BstSFI CTRYAG 5 cut(s) 43, 1265, 1474, 1521, 2560
BstV1I GCAGC 6 cut(s) 724, 1205, 1598, 1887, 2514, 2642
BstV2I GAAGAC 2 cut(s) 1190, 1456
BstX2I RGATCY 2 cut(s) 1148, 1685
BstXI CCANNNNNNTGG 1 cut(s) 1021
BstYI RGATCY 2 cut(s) 1148, 1685
BsuRI GGCC 6 cut(s) 91, 401, 726, 845, 1259, 1758
BtrI CACGTC 1 cut(s) 1454
BtsCI GGATG 8 cut(s) 74, 190, 760, 979, 1282, 1579, 1719, 2422
BtsI GCAGTG 1 cut(s) 2600
BtsIMutI CAGTG 3 cut(s) 368, 1935, 2600
BveI ACCTGC 4 cut(s) 232, 1235, 1258, 1760
Cac8I GCNNGC 6 cut(s) 357, 1294, 1406, 1971, 2194, 2676
CaiI CAGNNNCTG 4 cut(s) 520, 1611, 1903, 2468
CciI TCATGA 1 cut(s) 859
CfoI GCGC 2 cut(s) 250, 1605
Cfr13I GGNCC 3 cut(s) 89, 212, 221
CseI GACGC 1 cut(s) 743
Csp6I GTAC 8 cut(s) 376, 989, 1999, 2155, 2247, 2323, 2376, 2648
CviQI GTAC 8 cut(s) 376, 989, 1999, 2155, 2247, 2323, 2376, 2648
DdeI CTNAG 9 cut(s) 8, 63, 274, 351, 521, 933, 1920, 2651, 2670
DpnI GATC 9 cut(s) 630, 1019, 1150, 1335, 1392, 1506, 1687, 2384, 2686
DpnII GATC 9 cut(s) 628, 1017, 1148, 1333, 1390, 1504, 1685, 2382, 2684
EaeI YGGCCR 1 cut(s) 399
Eam1104I CTCTTC 3 cut(s) 758, 1453, 2115
EarI CTCTTC 3 cut(s) 758, 1453, 2115
EciI GGCGGA 2 cut(s) 557, 2566
Ecl136II GAGCTC 1 cut(s) 605
Eco130I CCWWGG 2 cut(s) 215, 717
Eco24I GRGCYC 1 cut(s) 607
Eco47I GGWCC 2 cut(s) 212, 221
Eco53kI GAGCTC 1 cut(s) 605
Eco57I CTGAAG 2 cut(s) 224, 1173
Eco88I CYCGRG 2 cut(s) 151, 1836
EcoICRI GAGCTC 1 cut(s) 605
EcoNI CCTNNNNNAGG 1 cut(s) 2624
EcoO109I RGGNCCY 1 cut(s) 221
EcoRII CCWGG 4 cut(s) 390, 401, 845, 1131
EcoT14I CCWWGG 2 cut(s) 215, 717
EcoT22I ATGCAT 2 cut(s) 481, 2680
EcoT38I GRGCYC 1 cut(s) 607
ErhI CCWWGG 2 cut(s) 215, 717
FalI AAGNNNNNCTT 2 cut(s) 755, 787
FaqI GGGAC 1 cut(s) 234
FauNDI CATATG 1 cut(s) 2527
FbaI TGATCA 2 cut(s) 1017, 1504
FblI GTMKAC 1 cut(s) 611
Fnu4HI GCNGC 7 cut(s) 738, 1194, 1260, 1612, 1901, 2503, 2631
FokI GGATG 8 cut(s) 61, 197, 767, 986, 1289, 1586, 1706, 2429
FriOI GRGCYC 1 cut(s) 607
Fsp4HI GCNGC 7 cut(s) 738, 1194, 1260, 1612, 1901, 2503, 2631
FspBI CTAG 5 cut(s) 665, 906, 941, 1496, 2448
FspI TGCGCA 1 cut(s) 1604
GlaI GCGC 2 cut(s) 249, 1604
GluI GCNGC 7 cut(s) 738, 1194, 1260, 1612, 1901, 2503, 2631
GsaI CCCAGC 2 cut(s) 575, 2406
GsuI CTGGAG 2 cut(s) 458, 1925
HaeIII GGCC 6 cut(s) 91, 401, 726, 845, 1259, 1758
HgaI GACGC 1 cut(s) 743
HhaI GCGC 2 cut(s) 250, 1605
Hin6I GCGC 2 cut(s) 248, 1603
HinP1I GCGC 2 cut(s) 248, 1603
HincII GTYRAC 3 cut(s) 612, 733, 979
HindII GTYRAC 3 cut(s) 612, 733, 979
HindIII AAGCTT 1 cut(s) 528
HphI GGTGA 5 cut(s) 72, 670, 1679, 1894, 2359
Hpy166II GTNNAC 8 cut(s) 612, 733, 979, 1321, 2155, 2157, 2249, 2323
Hpy188I TCNGA 7 cut(s) 127, 271, 597, 1098, 1153, 1504, 1509
Hpy8I GTNNAC 8 cut(s) 612, 733, 979, 1321, 2155, 2157, 2249, 2323
Hpy99I CGWCG 1 cut(s) 2029
HpyAV CCTTC 8 cut(s) 102, 199, 716, 1135, 1376, 1561, 2227, 2486
HpyCH4III ACNGT 9 cut(s) 131, 363, 682, 1318, 1939, 2246, 2258, 2365, 2375
HpyCH4IV ACGT 4 cut(s) 25, 1453, 2151, 2159
HpyF10VI GCNNNNNNNGC 6 cut(s) 245, 581, 1611, 1775, 1847, 2522
HpyF3I CTNAG 9 cut(s) 8, 63, 274, 351, 521, 933, 1920, 2651, 2670
HpySE526I ACGT 4 cut(s) 25, 1453, 2151, 2159
HspAI GCGC 2 cut(s) 248, 1603
Ksp22I TGATCA 2 cut(s) 1017, 1504
Kzo9I GATC 9 cut(s) 628, 1017, 1148, 1333, 1390, 1504, 1685, 2382, 2684
LguI GCTCTTC 1 cut(s) 758
LmnI GCTCC 5 cut(s) 9, 602, 661, 1635, 1734
Lsp1109I GCAGC 6 cut(s) 724, 1205, 1598, 1887, 2514, 2642
MaeI CTAG 5 cut(s) 665, 906, 941, 1496, 2448
MaeII ACGT 4 cut(s) 25, 1453, 2151, 2159
MaeIII GTNAC 7 cut(s) 510, 676, 895, 1417, 1577, 1942, 2365
MalI GATC 9 cut(s) 630, 1019, 1150, 1335, 1392, 1506, 1687, 2384, 2686
MbiI CCGCTC 1 cut(s) 1262
MboI GATC 9 cut(s) 628, 1017, 1148, 1333, 1390, 1504, 1685, 2382, 2684
MflI RGATCY 2 cut(s) 1148, 1685
MhlI GDGCHC 1 cut(s) 607
MlsI TGGCCA 1 cut(s) 401
MluNI TGGCCA 1 cut(s) 401
MlyI GAGTC 4 cut(s) 294, 634, 1332, 2591
MmeI TCCRAC 2 cut(s) 294, 732
Mox20I TGGCCA 1 cut(s) 401
Mph1103I ATGCAT 2 cut(s) 481, 2680
MroXI GAANNNNTTC 3 cut(s) 432, 1550, 2211
MscI TGGCCA 1 cut(s) 401
MslI CAYNNNNRTG 1 cut(s) 1019
Msp20I TGGCCA 1 cut(s) 401
MspA1I CMGCKG 2 cut(s) 1900, 2505
MspCI CTTAAG 1 cut(s) 289
MspR9I CCNGG 4 cut(s) 392, 403, 847, 1133
Mva1269I GAATGC 5 cut(s) 241, 639, 1092, 2457, 2621
MvaI CCWGG 4 cut(s) 392, 403, 847, 1133
MwoI GCNNNNNNNGC 6 cut(s) 245, 581, 1611, 1775, 1847, 2522
NdeI CATATG 1 cut(s) 2527
NdeII GATC 9 cut(s) 628, 1017, 1148, 1333, 1390, 1504, 1685, 2382, 2684
NlaIV GGNNCC 1 cut(s) 222
NmeAIII GCCGAG 2 cut(s) 752, 2527
NmuCI GTSAC 5 cut(s) 510, 676, 895, 1577, 2365
NsbI TGCGCA 1 cut(s) 1604
NsiI ATGCAT 2 cut(s) 481, 2680
NspI RCATGY 7 cut(s) 483, 1234, 1371, 1408, 2005, 2583, 2678
PaeI GCATGC 2 cut(s) 1408, 2678
PaeR7I CTCGAG 2 cut(s) 151, 1836
PagI TCATGA 1 cut(s) 859
PciI ACATGT 1 cut(s) 2579
PciSI GCTCTTC 1 cut(s) 758
PctI GAATGC 5 cut(s) 241, 639, 1092, 2457, 2621
PdmI GAANNNNTTC 3 cut(s) 432, 1550, 2211
PfeI GAWTC 7 cut(s) 39, 119, 1499, 1510, 1716, 1863, 2465
PkrI GCNGC 7 cut(s) 739, 1195, 1261, 1613, 1902, 2504, 2632
PleI GAGTC 4 cut(s) 293, 634, 1332, 2591
PpsI GAGTC 4 cut(s) 293, 634, 1332, 2591
Ppu21I YACGTR 1 cut(s) 2160
PpuMI RGGWCCY 1 cut(s) 221
PscI ACATGT 1 cut(s) 2579
PshBI ATTAAT 3 cut(s) 321, 1748, 2568
PsiI TTATAA 4 cut(s) 798, 1008, 2268, 2478
Psp124BI GAGCTC 1 cut(s) 607
Psp5II RGGWCCY 1 cut(s) 221
Psp6I CCWGG 4 cut(s) 390, 401, 845, 1131
PspFI CCCAGC 2 cut(s) 571, 2402
PspGI CCWGG 4 cut(s) 390, 401, 845, 1131
PspN4I GGNNCC 1 cut(s) 222
PspPI GGNCC 3 cut(s) 89, 212, 221
PspPPI RGGWCCY 1 cut(s) 221
PspXI VCTCGAGB 2 cut(s) 151, 1836
PstI CTGCAG 1 cut(s) 1269
PstNI CAGNNNCTG 4 cut(s) 520, 1611, 1903, 2468
PsuI RGATCY 2 cut(s) 1148, 1685
PvuII CAGCTG 1 cut(s) 1900
RsaI GTAC 8 cut(s) 377, 990, 2000, 2156, 2248, 2324, 2377, 2649
RsaNI GTAC 8 cut(s) 376, 989, 1999, 2155, 2247, 2323, 2376, 2648
RseI CAYNNNNRTG 1 cut(s) 1019
SacI GAGCTC 1 cut(s) 607
SalI GTCGAC 1 cut(s) 610
SapI GCTCTTC 1 cut(s) 758
SatI GCNGC 7 cut(s) 738, 1194, 1260, 1612, 1901, 2503, 2631
Sau3AI GATC 9 cut(s) 628, 1017, 1148, 1333, 1390, 1504, 1685, 2382, 2684
Sau96I GGNCC 3 cut(s) 89, 212, 221
SchI GAGTC 4 cut(s) 294, 634, 1332, 2591
ScrFI CCNGG 4 cut(s) 392, 403, 847, 1133
SduI GDGCHC 1 cut(s) 607
SfcI CTRYAG 5 cut(s) 43, 1265, 1474, 1521, 2560
Sfr274I CTCGAG 2 cut(s) 151, 1836
SinI GGWCC 2 cut(s) 212, 221
SlaI CTCGAG 2 cut(s) 151, 1836
SmiMI CAYNNNNRTG 1 cut(s) 1019
SmlI CTYRAG 5 cut(s) 151, 230, 289, 1710, 1836
SmoI CTYRAG 5 cut(s) 151, 230, 289, 1710, 1836
SphI GCATGC 2 cut(s) 1408, 2678
SsiI CCGC 4 cut(s) 568, 1260, 2505, 2551
SspI AATATT 3 cut(s) 1204, 1746, 2272
SspMI CTAG 5 cut(s) 665, 906, 941, 1496, 2448
SstI GAGCTC 1 cut(s) 607
StyD4I CCNGG 4 cut(s) 390, 401, 845, 1131
StyI CCWWGG 2 cut(s) 215, 717
TaaI ACNGT 9 cut(s) 131, 363, 682, 1318, 1939, 2246, 2258, 2365, 2375
TaiI ACGT 4 cut(s) 28, 1456, 2154, 2162
TaqII GACCGA 1 cut(s) 299
TatI WGTACW 4 cut(s) 2154, 2246, 2322, 2647
TauI GCSGC 1 cut(s) 1262
TfiI GAWTC 7 cut(s) 39, 119, 1499, 1510, 1716, 1863, 2465
TscAI CASTG 3 cut(s) 368, 1942, 2607
TseFI GTSAC 5 cut(s) 510, 676, 895, 1577, 2365
TseI GCWGC 6 cut(s) 737, 1193, 1611, 1900, 2502, 2630
Tsp45I GTSAC 5 cut(s) 510, 676, 895, 1577, 2365
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 3 cut(s) 368, 1942, 2607
Vha464I CTTAAG 1 cut(s) 289
VpaK11BI GGWCC 2 cut(s) 212, 221
VspI ATTAAT 3 cut(s) 321, 1748, 2568
XagI CCTNNNNNAGG 1 cut(s) 2624
XapI RAATTY 4 cut(s) 412, 563, 851, 2303
XceI RCATGY 7 cut(s) 483, 1234, 1371, 1408, 2005, 2583, 2678
XhoI CTCGAG 2 cut(s) 151, 1836
XmiI GTMKAC 1 cut(s) 611
XmnI GAANNNNTTC 3 cut(s) 432, 1550, 2211
XspI CTAG 5 cut(s) 665, 906, 941, 1496, 2448
Zsp2I ATGCAT 2 cut(s) 481, 2680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.