MD08G1073000.v1.1

Guanine nucleotide-binding protein alpha-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006871
Physical Location & Seq
Reverse (-)
5899121 .. 5903670
4550 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1073000.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGGGCATACTCTGCAGTAGAAACAAACATTACGACGAAGCGGACGACGAAGAGAATGCTCAGACTGCAGAAATTGAAAGGCGAATTGAACAAGAAACGAAGGCCGAAAAGCATATTCAGAAACTTCTACTACTTGGTGCTGGGGAATCCGGGAAGTCCACAATTTTTAAGCAGATAAAGCTTTTGTTTCAAACTGGATTTGATGAGTCCGAGCTCAAGAGCTACATCTCAGTCATCCATGCCAATGTATATCAGACCATAAAAGTACTGTATGATGGGTCAAAGGAGCTTGCTCAGAACGACAGAGAAACGTTTGAAATATCCAGTGAAAATAAGGAAATTGGAGAGAAACTATCAACAATTGGCAGCAGATTGGATTATCCATGTCTCACTAAAGAGCTTGCACAGGATATAGAGACTCTTTGGAAAGATGCGGCAATTCAGGAAACATGTGCTCGTGGTAATGAACTCCAAGTTCCTGATTGTGCCCACTATTTCATGGAGAATCTTCAAAGATTAGCTGACCCAGATTATGTTCCAACCAAGGAGGATGTTCTTCATGCGAGAGTTCGTACAACTGGTGTCGTAGAGATCCAGTTCAGCCCTGTTGGAGAAAATAAGAAAAGCGGTGAAGTATATCGACTCTTTGATGTTGGAGGCCAGAGAAATGAGAGAAGGAAATGGATCCATCTATTTGAAGGCGTTACAGCTGTAATATTTTGTGCCGCAGTTAGCGAGTATGATCAAACACTCTTCGAGGATGAAAGCAAAAATCGAATGATGGAGACAAAGGAACTTTTTGAGTGGGTCTTGAAGCAACCGTGTTTTGAGAAAACGTCGTTCATGCTATTCTTAAACAAGTTCGATATATTCGAGAAGAAAGTTCTAAACGTGCCACTTAACGTCTGCGAGTGGTTCAAAGATTACCAGCCGGTGTCAACAGGAAAGCAAGAGATCGAACATGCGTATGAGTTTGTGAAGAAGAAATTCGAGGAACTGTACTTTCAGAGCACGGCCCCTGACCGCGTAGACAGGGTGTTTAAGATCTACAGAACCACTGCCCTTGATCAGAAGCTTGTGAAGAAGACTTTCAAGCTCGTAGACGAGACGTTGAGACGGAGAAATCTCTTCGAGGCCGGATTATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0001101 GO:0001664 GO:0001789 GO:0001882 GO:0001883 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0003924 GO:0004857 GO:0005095 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005834 GO:0005886 GO:0005911 GO:0006082 GO:0006520 GO:0006558 GO:0006570 GO:0006571 GO:0006725 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009094 GO:0009095 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009637 GO:0009642 GO:0009645 GO:0009657 GO:0009668 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009739 GO:0009740 GO:0009741 GO:0009742 GO:0009743 GO:0009746 GO:0009749 GO:0009755 GO:0009785 GO:0009787 GO:0009788 GO:0009789 GO:0009791 GO:0009845 GO:0009870 GO:0009898 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010027 GO:0010033 GO:0010119 GO:0010244 GO:0010476 GO:0010646 GO:0010647 GO:0010648 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016053 GO:0016247 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0017144 GO:0019001 GO:0019438 GO:0019752 GO:0019897 GO:0019898 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030054 GO:0030234 GO:0030522 GO:0030695 GO:0031234 GO:0031347 GO:0031349 GO:0031683 GO:0031984 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032870 GO:0032991 GO:0033993 GO:0034260 GO:0034284 GO:0035639 GO:0036094 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0043086 GO:0043087 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043401 GO:0043436 GO:0044092 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0044877 GO:0045088 GO:0045089 GO:0045927 GO:0046394 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0048583 GO:0048584 GO:0048585 GO:0048638 GO:0048639 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051336 GO:0051346 GO:0051716 GO:0055044 GO:0060589 GO:0061024 GO:0065007 GO:0065009 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071367 GO:0071370 GO:0071383 GO:0071396 GO:0071407 GO:0071478 GO:0071482 GO:0071483 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0072593 GO:0080134 GO:0090351 GO:0097159 GO:0097305 GO:0097306 GO:0097367 GO:0098552 GO:0098562 GO:0098772 GO:0098796 GO:0098797 GO:0098827 GO:0104004 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901419 GO:1901420 GO:1901421 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901700 GO:1901701 GO:1902221 GO:1902223 GO:1902494 GO:1905360 GO:1905957 GO:1905958 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04742 ko04973 map04742 map04973
Pfam Domains
Protein Families
G-alpha

Protein Analysis

383

Amino Acids

44.51

Weight (kDa)

5.39

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-alpha PF00503 21 - 370 2e-108 G-protein alpha subunit
Arf PF00025 213 - 292 2.7e-07 ADP-ribosylation factor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 1029, 1101
AccII CGCG 1 cut(s) 1026
AciI CCGC 5 cut(s) 41, 434, 627, 726, 1024
AclI AACGTT 1 cut(s) 311
AclWI GGATC 3 cut(s) 586, 679, 692
AcsI RAATTY 1 cut(s) 986
AfaI GTAC 3 cut(s) 267, 574, 1001
AflIII ACRYGT 1 cut(s) 449
AgsI TTSAA 9 cut(s) 77, 89, 191, 317, 512, 698, 814, 919, 1093
AluBI AGCT 9 cut(s) 181, 214, 222, 289, 400, 521, 710, 1075, 1096
AluI AGCT 9 cut(s) 181, 214, 222, 289, 400, 521, 710, 1075, 1096
Alw21I GWGCWC 3 cut(s) 216, 457, 1013
Alw26I GTCTC 5 cut(s) 392, 410, 779, 1100, 1108
AlwI GGATC 3 cut(s) 586, 679, 692
AoxI GGCC 4 cut(s) 102, 658, 1014, 1134
ApeKI GCWGC 1 cut(s) 366
ApoI RAATTY 1 cut(s) 986
Asp700I GAANNNNTTC 2 cut(s) 986, 1088
AspS9I GGNCC 1 cut(s) 1015
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 1 cut(s) 641
BaeGI GKGCMC 1 cut(s) 490
BamHI GGATCC 1 cut(s) 684
BanII GRGCYC 1 cut(s) 216
BauI CACGAG 1 cut(s) 456
BbsI GAAGAC 1 cut(s) 1091
Bbv12I GWGCWC 3 cut(s) 216, 457, 1013
BbvI GCAGC 1 cut(s) 378
BccI CCATC 3 cut(s) 269, 696, 775
BceAI ACGGC 1 cut(s) 1029
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BclI TGATCA 2 cut(s) 742, 1066
BcnI CCSGG 1 cut(s) 151
BcoDI GTCTC 5 cut(s) 392, 410, 779, 1100, 1108
BfmI CTRYAG 3 cut(s) 13, 66, 1048
BglII AGATCT 1 cut(s) 1044
BisI GCNGC 3 cut(s) 367, 435, 726
BlsI GCNGC 3 cut(s) 368, 436, 727
BmcAI AGTACT 1 cut(s) 267
Bme1390I CCNGG 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 1015
BmiI GGNNCC 2 cut(s) 686, 1017
BmrFI CCNGG 1 cut(s) 151
BmsI GCATC 1 cut(s) 421
BpiI GAAGAC 1 cut(s) 1091
BpuEI CTTGAG 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 151
BsaJI CCNNGG 1 cut(s) 543
Bse118I RCCGGY 1 cut(s) 931
Bse1I ACTGG 4 cut(s) 199, 324, 583, 595
BseDI CCNNGG 1 cut(s) 543
BseGI GGATG 3 cut(s) 234, 556, 766
BseMII CTCAG 3 cut(s) 74, 243, 308
BseNI ACTGG 4 cut(s) 199, 324, 583, 595
BseSI GKGCMC 1 cut(s) 490
BseXI GCAGC 1 cut(s) 378
BseYI CCCAGC 1 cut(s) 140
Bsh1236I CGCG 1 cut(s) 1026
BshFI GGCC 4 cut(s) 104, 660, 1016, 1136
BsiHKAI GWGCWC 3 cut(s) 216, 457, 1013
BsiSI CCGG 3 cut(s) 150, 932, 1137
BsmAI GTCTC 5 cut(s) 392, 410, 779, 1100, 1108
BsmBI CGTCTC 2 cut(s) 1100, 1108
BsmI GAATGC 1 cut(s) 61
BsnI GGCC 4 cut(s) 104, 660, 1016, 1136
Bsp1286I GDGCHC 4 cut(s) 216, 457, 490, 1013
Bsp143I GATC 6 cut(s) 591, 684, 742, 954, 1044, 1066
BspACI CCGC 5 cut(s) 41, 434, 627, 726, 1024
BspANI GGCC 4 cut(s) 104, 660, 1016, 1136
BspCNI CTCAG 3 cut(s) 73, 242, 307
BspFNI CGCG 1 cut(s) 1026
BspLI GGNNCC 2 cut(s) 686, 1017
BspMAI CTGCAG 2 cut(s) 17, 70
BspPI GGATC 3 cut(s) 586, 679, 692
BsrFI RCCGGY 1 cut(s) 931
BsrI ACTGG 4 cut(s) 199, 324, 583, 595
BssAI RCCGGY 1 cut(s) 931
BssECI CCNNGG 1 cut(s) 543
BssMI GATC 6 cut(s) 591, 684, 742, 954, 1044, 1066
BssSI CACGAG 1 cut(s) 456
BssT1I CCWWGG 1 cut(s) 543
Bst2BI CACGAG 1 cut(s) 456
Bst4CI ACNGT 3 cut(s) 270, 822, 999
Bst6I CTCTTC 3 cut(s) 45, 758, 1133
BstAPI GCANNNNNTGC 1 cut(s) 12
BstC8I GCNNGC 2 cut(s) 291, 402
BstDEI CTNAG 3 cut(s) 60, 229, 294
BstF5I GGATG 3 cut(s) 234, 556, 766
BstFNI CGCG 1 cut(s) 1026
BstKTI GATC 6 cut(s) 594, 687, 745, 957, 1047, 1069
BstMAI GTCTC 5 cut(s) 392, 410, 779, 1100, 1108
BstMBI GATC 6 cut(s) 591, 684, 742, 954, 1044, 1066
BstMWI GCNNNNNNNGC 3 cut(s) 12, 65, 178
BstNSI RCATGY 2 cut(s) 453, 965
BstSCI CCNGG 1 cut(s) 149
BstSFI CTRYAG 3 cut(s) 13, 66, 1048
BstSLI GKGCMC 1 cut(s) 490
BstUI CGCG 1 cut(s) 1026
BstV1I GCAGC 1 cut(s) 378
BstV2I GAAGAC 1 cut(s) 1091
BstX2I RGATCY 3 cut(s) 591, 684, 1044
BstYI RGATCY 3 cut(s) 591, 684, 1044
BsuRI GGCC 4 cut(s) 104, 660, 1016, 1136
BtsCI GGATG 3 cut(s) 234, 556, 766
BtsI GCAGTG 1 cut(s) 1056
BtsIMutI CAGTG 2 cut(s) 331, 1056
Cac8I GCNNGC 2 cut(s) 291, 402
Cfr10I RCCGGY 1 cut(s) 931
Cfr13I GGNCC 1 cut(s) 1015
Csp6I GTAC 3 cut(s) 266, 573, 1000
CspCI CAANNNNNGTGG 2 cut(s) 1045, 1080
CviAII CATG 7 cut(s) 239, 384, 450, 499, 560, 844, 962
CviQI GTAC 3 cut(s) 266, 573, 1000
DdeI CTNAG 3 cut(s) 60, 229, 294
DpnI GATC 6 cut(s) 593, 686, 744, 956, 1046, 1068
DpnII GATC 6 cut(s) 591, 684, 742, 954, 1044, 1066
Eam1104I CTCTTC 3 cut(s) 45, 758, 1133
EarI CTCTTC 3 cut(s) 45, 758, 1133
Ecl136II GAGCTC 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 543
Eco24I GRGCYC 1 cut(s) 216
Eco53kI GAGCTC 1 cut(s) 214
EcoICRI GAGCTC 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 543
EcoT38I GRGCYC 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 543
Esp3I CGTCTC 2 cut(s) 1100, 1108
FaeI CATG 7 cut(s) 242, 387, 453, 502, 563, 847, 965
FatI CATG 7 cut(s) 238, 383, 449, 498, 559, 843, 961
FbaI TGATCA 2 cut(s) 742, 1066
FblI GTMKAC 2 cut(s) 1029, 1101
Fnu4HI GCNGC 3 cut(s) 367, 435, 726
FokI GGATG 3 cut(s) 221, 563, 773
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 3 cut(s) 367, 435, 726
GluI GCNGC 3 cut(s) 367, 435, 726
GsaI CCCAGC 1 cut(s) 144
HaeIII GGCC 4 cut(s) 104, 660, 1016, 1136
HapII CCGG 3 cut(s) 150, 932, 1137
Hin1II CATG 7 cut(s) 242, 387, 453, 502, 563, 847, 965
HincII GTYRAC 1 cut(s) 939
HindII GTYRAC 1 cut(s) 939
HindIII AAGCTT 2 cut(s) 179, 1073
HinfI GANTC 5 cut(s) 146, 206, 418, 505, 642
HpaII CCGG 3 cut(s) 150, 932, 1137
HphI GGTGA 1 cut(s) 641
Hpy166II GTNNAC 4 cut(s) 159, 939, 1030, 1102
Hpy188I TCNGA 7 cut(s) 63, 120, 211, 255, 297, 1008, 1071
Hpy188III TCNNGA 5 cut(s) 217, 443, 479, 811, 874
Hpy8I GTNNAC 4 cut(s) 159, 939, 1030, 1102
Hpy99I CGWCG 3 cut(s) 38, 50, 841
HpyAV CCTTC 3 cut(s) 94, 669, 692
HpyCH4III ACNGT 3 cut(s) 270, 822, 999
HpyCH4IV ACGT 5 cut(s) 311, 836, 891, 903, 1109
HpyCH4V TGCA 3 cut(s) 15, 68, 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 12, 65, 178
HpyF3I CTNAG 3 cut(s) 60, 229, 294
HpySE526I ACGT 5 cut(s) 311, 836, 891, 903, 1109
Hsp92II CATG 7 cut(s) 242, 387, 453, 502, 563, 847, 965
Ksp22I TGATCA 2 cut(s) 742, 1066
Kzo9I GATC 6 cut(s) 591, 684, 742, 954, 1044, 1066
LmnI GCTCC 1 cut(s) 286
Lsp1109I GCAGC 1 cut(s) 378
LweI GCATC 1 cut(s) 421
MaeII ACGT 5 cut(s) 311, 836, 891, 903, 1109
MaeIII GTNAC 1 cut(s) 703
MalI GATC 6 cut(s) 593, 686, 744, 956, 1046, 1068
MboI GATC 6 cut(s) 591, 684, 742, 954, 1044, 1066
MfeI CAATTG 1 cut(s) 360
MflI RGATCY 3 cut(s) 591, 684, 1044
MhlI GDGCHC 4 cut(s) 216, 457, 490, 1013
MluCI AATT 7 cut(s) 72, 84, 162, 339, 360, 438, 986
MlyI GAGTC 3 cut(s) 215, 412, 636
MmeI TCCRAC 3 cut(s) 563, 589, 634
MnlI CCTC 5 cut(s) 541, 650, 751, 985, 1126
MroXI GAANNNNTTC 2 cut(s) 986, 1088
MseI TTAA 4 cut(s) 168, 854, 900, 1041
MslI CAYNNNNRTG 2 cut(s) 243, 966
MspA1I CMGCKG 1 cut(s) 710
MspI CCGG 3 cut(s) 150, 932, 1137
MspR9I CCNGG 1 cut(s) 151
MunI CAATTG 1 cut(s) 360
Mva1269I GAATGC 1 cut(s) 61
MvnI CGCG 1 cut(s) 1026
MwoI GCNNNNNNNGC 3 cut(s) 12, 65, 178
NciI CCSGG 1 cut(s) 151
NdeII GATC 6 cut(s) 591, 684, 742, 954, 1044, 1066
NlaIII CATG 7 cut(s) 242, 387, 453, 502, 563, 847, 965
NlaIV GGNNCC 2 cut(s) 686, 1017
NspI RCATGY 2 cut(s) 453, 965
PciI ACATGT 1 cut(s) 449
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 870
PctI GAATGC 1 cut(s) 61
PdmI GAANNNNTTC 2 cut(s) 986, 1088
PfeI GAWTC 2 cut(s) 146, 505
PfoI TCCNGGA 1 cut(s) 149
PkrI GCNGC 3 cut(s) 368, 436, 727
PleI GAGTC 3 cut(s) 214, 412, 636
PpsI GAGTC 3 cut(s) 214, 412, 636
PscI ACATGT 1 cut(s) 449
Psp124BI GAGCTC 1 cut(s) 216
Psp1406I AACGTT 1 cut(s) 311
PspFI CCCAGC 1 cut(s) 140
PspN4I GGNNCC 2 cut(s) 686, 1017
PspPI GGNCC 1 cut(s) 1015
PstI CTGCAG 2 cut(s) 17, 70
PsuI RGATCY 3 cut(s) 591, 684, 1044
PvuII CAGCTG 1 cut(s) 710
RsaI GTAC 3 cut(s) 267, 574, 1001
RsaNI GTAC 3 cut(s) 266, 573, 1000
RseI CAYNNNNRTG 2 cut(s) 243, 966
SacI GAGCTC 1 cut(s) 216
SaqAI TTAA 4 cut(s) 168, 854, 900, 1041
SatI GCNGC 3 cut(s) 367, 435, 726
Sau3AI GATC 6 cut(s) 591, 684, 742, 954, 1044, 1066
Sau96I GGNCC 1 cut(s) 1015
ScaI AGTACT 1 cut(s) 267
SchI GAGTC 3 cut(s) 215, 412, 636
ScrFI CCNGG 1 cut(s) 151
SduI GDGCHC 4 cut(s) 216, 457, 490, 1013
SfaNI GCATC 1 cut(s) 421
SfcI CTRYAG 3 cut(s) 13, 66, 1048
SmiMI CAYNNNNRTG 2 cut(s) 243, 966
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 7 cut(s) 72, 84, 162, 339, 360, 438, 986
SsiI CCGC 5 cut(s) 41, 434, 627, 726, 1024
SspI AATATT 1 cut(s) 717
SstI GAGCTC 1 cut(s) 216
StyD4I CCNGG 1 cut(s) 149
StyI CCWWGG 1 cut(s) 543
TaaI ACNGT 3 cut(s) 270, 822, 999
TaiI ACGT 5 cut(s) 314, 839, 894, 906, 1112
TaqI TCGA 8 cut(s) 640, 756, 775, 864, 873, 957, 990, 1131
TasI AATT 7 cut(s) 72, 84, 162, 339, 360, 438, 986
TatI WGTACW 2 cut(s) 265, 999
TauI GCSGC 2 cut(s) 437, 728
TfiI GAWTC 2 cut(s) 146, 505
Tru1I TTAA 4 cut(s) 168, 854, 900, 1041
Tru9I TTAA 4 cut(s) 168, 854, 900, 1041
TscAI CASTG 2 cut(s) 331, 1063
TseI GCWGC 1 cut(s) 366
TspDTI ATGAA 5 cut(s) 480, 487, 548, 777, 832
TspGWI ACGGA 1 cut(s) 1132
TspRI CASTG 2 cut(s) 331, 1063
XapI RAATTY 1 cut(s) 986
XceI RCATGY 2 cut(s) 453, 965
XmiI GTMKAC 2 cut(s) 1029, 1101
XmnI GAANNNNTTC 2 cut(s) 986, 1088
ZrmI AGTACT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.