Rorug06G0391900

Guanine nucleotide-binding protein alpha-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006871
Physical Location & Seq
Reverse (-)
53660173 .. 53664864
4692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0391900.1

Sequence Viewer

Length: 2097 bp
ATGGACAGGCGACAAGTGCAATTGATAGTCAATTCTGGATATGTTGCCACTGCCACAACTAACACAAGTGCAACACTTCTTGACACTGGAAATTTGGTCCTTAAGGAAGCAGATACAGGTACTATTATATGGCAGAGTTTTGATATTCCAAGCGATACGTATCTTCCTGGTATGAAACTTGGGTTGTTTGGCCTAAACAGTACAAACAAGCCAGGCTTTCACGTGCTTGTTTCTTGGGTAAGCCCCAAAGACCCTGCTCGTGGCCTCTTCACCTTAACTGTTGATGGCACCAACTTAACAAAGCTTAAAGTTTGGCGAGGAGATGGAGCAAAAATGGATATTGCTTTTTGGGACGGACATGGGTTACAGTTTGTTTTTGAGAACTCAACAGGGAATAATAACTACAACTTTAGCTACCAGTCCAATAAATATGAGGCCTATTACACTTTTAGCAGCAGTCAGAAGTATGATCTCATGTGGTTTGTATTGGCTTCCACTGGAAACCTTGAACAGTATGTTATGTTGGACAAGAAGATTTCTTCTGTGAGTCATACTTTGTGTGAGGATGGTGGGATGTGCTTGACTTCAATACCCTCTAAGTGTGACGATGGTGGTAATTTTCTTGAGGTGAATGGTTCTTTGCCATCTACATATAATAGTGGATCCATCAATATGAGGGCCAGTGATTGTGAAATTTTATGCAAGAACAATTGTTCTTGTTCCGCATTTGCTTCAGTTCAGAATGAAGAAACTGGATGTCAACTTTATTATGGGAGTAAACGTGAATTATTGAAGATTATAGAGAAGGGTCCAGGGATTATTTACATCCGCGGTGGCACTCCAAGTGATAGTAAGAAGTGGAAGCTGTGGTTGGCTGTTGCAGTTCCTTTGGCTTTCCTCTTGGCTCTTATTCCAATCTCCTTGTCATGCTATTTGTGTCGACGTAAAGAAGATCAGGTAACCAGGCGGAAAGGCTTTTGTGATCAAGTAAGATTATTCCAAATGGGCTCCAATTTTGCATCAACAGTTCCCCATGATGAAGCTGGAGGAGGTGCAGATAACATTGAAATAGGCAGACAGAAGGATCAAGAATTGCCCTTATTTAGTTTTTCTACCATAAAGACTGCAACAGATAACTTTGCAGATACTAATAAACTGGGGGAAGGTGGATTTGGGCCTGTCTATAAGGGCAAGTTGCTAGTAGGACAGGAAATTGCAGTCAAAAGGCTGTCCAAAATTTCCCGGCAAGGATTGAGGGAGTTCAAAAATGAAGTCTCAGTAATTTGTAAGCTCCAGCACAGGAATTTGGTTCGGCTTTTGGGGTGCTGCATTGAAGCAGAAGAGAGTATACTAATTTATGAGTACATGCCCAACAAAAGTCTGGATTGCTTCATTTTTGATTCAACCAACCGGGCACTTATGGATTGGAGGAGGTGTATTGGCATCATTGAAGGGATTGCTCAAGGTCTTCTCTATCTTCATAAATACTCAAGATTAAGGATCATTCACCGTGATTTAAAGACCAGCAATATACTGTTGGACAGTGAGATGAACCCGAAAATTTCTGATTTTGGCATGGCTAGAATTTTTGGGGATGACGACAGTAGAGGAAAAACGAATCGGGTTGTTGGTACATTTGGTTACATGTCTCCTGAGTACGCCATGGACGGCCTGTTTTTTGAAAAATCAGATGTCTTTAGCTTTGGGGTGATCATATTAGAGATCATAAGTTGCAAGAAGAACATTGCCTTCTTTGAGACTGATCATTCTTTGAACTTACTTGGAAAAGCTTGGTATTTGTGGAAAGAAGGCAATGGCATGGACTTGATTGATTCTACTCTCTGCGCTTCTTGTTCAAGCAGTGAAGTTATGAGATGCATACAAATGGGTCTTTTGTGCGTCCAAGAAAGAGCTATGGATCGACCAAATATGTCAGATGTTGTTTCGATGCTAAGCAATGAATCAATTGCTCTGCCTGTTCCTAAAGAACCTGCATTTTTGAATCAGCTAAATTCTGCTAATGCAGATATATCTTCAAGTAAGCAAAGATGTAGCTGTAACAATGATCTAACAGTTTCCTCAGAACATGCCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0001101 GO:0001664 GO:0001789 GO:0001882 GO:0001883 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0003924 GO:0004857 GO:0005095 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005834 GO:0005886 GO:0005911 GO:0006082 GO:0006520 GO:0006558 GO:0006570 GO:0006571 GO:0006725 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009094 GO:0009095 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009637 GO:0009642 GO:0009645 GO:0009657 GO:0009668 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009739 GO:0009740 GO:0009741 GO:0009742 GO:0009743 GO:0009746 GO:0009749 GO:0009755 GO:0009785 GO:0009787 GO:0009788 GO:0009789 GO:0009791 GO:0009845 GO:0009870 GO:0009898 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010027 GO:0010033 GO:0010119 GO:0010244 GO:0010476 GO:0010646 GO:0010647 GO:0010648 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016053 GO:0016247 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0017144 GO:0019001 GO:0019438 GO:0019752 GO:0019897 GO:0019898 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030054 GO:0030234 GO:0030522 GO:0030695 GO:0031234 GO:0031347 GO:0031349 GO:0031683 GO:0031984 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032870 GO:0032991 GO:0033993 GO:0034260 GO:0034284 GO:0035639 GO:0036094 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0043086 GO:0043087 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043401 GO:0043436 GO:0044092 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0044877 GO:0045088 GO:0045089 GO:0045927 GO:0046394 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0048583 GO:0048584 GO:0048585 GO:0048638 GO:0048639 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051336 GO:0051346 GO:0051716 GO:0055044 GO:0060589 GO:0061024 GO:0065007 GO:0065009 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071367 GO:0071370 GO:0071383 GO:0071396 GO:0071407 GO:0071478 GO:0071482 GO:0071483 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0072593 GO:0080134 GO:0090351 GO:0097159 GO:0097305 GO:0097306 GO:0097367 GO:0098552 GO:0098562 GO:0098772 GO:0098796 GO:0098797 GO:0098827 GO:0104004 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901419 GO:1901420 GO:1901421 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901700 GO:1901701 GO:1902221 GO:1902223 GO:1902494 GO:1905360 GO:1905957 GO:1905958 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04742 ko04973 map04742 map04973
Pfam Domains
Protein Families
G-alpha

Protein Analysis

698

Amino Acids

77.68

Weight (kDa)

6.36

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 10 - 63 9.9e-15 D-mannose binding lectin
Pkinase PF00069 382 - 580 2.4e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 384 - 650 6.3e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1999
AccB1I GGYRCC 1 cut(s) 287
AccI GTMKAC 2 cut(s) 940, 1348
AccII CGCG 1 cut(s) 831
AciI CCGC 4 cut(s) 723, 829, 831, 967
AclWI GGATC 5 cut(s) 657, 670, 1092, 1508, 1926
AcsI RAATTY 7 cut(s) 91, 693, 1236, 1303, 1560, 1584, 2011
AcuI CTGAAG 1 cut(s) 717
AcvI CACGTG 1 cut(s) 223
AfaI GTAC 5 cut(s) 121, 202, 1364, 1633, 1658
AfiI CCNNNNNNNGG 1 cut(s) 260
AflII CTTAAG 1 cut(s) 101
AflIII ACRYGT 1 cut(s) 1644
AjnI CCWGG 5 cut(s) 166, 211, 811, 962, 2090
AjuI GAANNNNNNNTTGG 4 cut(s) 854, 886, 1155, 1187
Alw26I GTCTC 3 cut(s) 1279, 1653, 1751
AlwI GGATC 5 cut(s) 657, 670, 1092, 1508, 1926
AoxI GGCC 6 cut(s) 190, 262, 435, 678, 1175, 1669
ApeKI GCWGC 2 cut(s) 453, 1326
ApoI RAATTY 7 cut(s) 91, 693, 1236, 1303, 1560, 1584, 2011
ArsI GACNNNNNNTTYG 2 cut(s) 1257, 1289
AspLEI GCGC 1 cut(s) 1847
AspS9I GGNCC 4 cut(s) 97, 678, 809, 1175
AsuC2I CCSGG 2 cut(s) 1243, 1412
AsuHPI GGTGA 4 cut(s) 262, 640, 1499, 1720
AvaII GGWCC 2 cut(s) 97, 809
BaeGI GKGCMC 1 cut(s) 1417
BamHI GGATCC 1 cut(s) 662
BanI GGYRCC 1 cut(s) 287
BanII GRGCYC 1 cut(s) 1010
BarI GAAGNNNNNNTAC 2 cut(s) 1332, 1364
BauI CACGAG 1 cut(s) 258
BbrPI CACGTG 1 cut(s) 223
BbsI GAAGAC 1 cut(s) 1460
BbvI GCAGC 2 cut(s) 465, 1313
BccI CCATC 6 cut(s) 278, 317, 560, 602, 652, 674
BceAI ACGGC 1 cut(s) 1684
BciT130I CCWGG 5 cut(s) 168, 213, 813, 964, 2092
BclI TGATCA 3 cut(s) 982, 1710, 1762
BcnI CCSGG 2 cut(s) 1243, 1412
BcoDI GTCTC 3 cut(s) 1279, 1653, 1751
BfaI CTAG 2 cut(s) 1199, 1581
BfrI CTTAAG 1 cut(s) 101
BfuAI ACCTGC 1 cut(s) 1999
BisI GCNGC 2 cut(s) 454, 1327
BlpI GCTNAGC 1 cut(s) 1952
BlsI GCNGC 2 cut(s) 455, 1328
Bme1390I CCNGG 7 cut(s) 168, 213, 813, 964, 1243, 1412, 2092
Bme18I GGWCC 2 cut(s) 97, 809
BmgT120I GGNCC 4 cut(s) 97, 678, 809, 1175
BmiI GGNNCC 4 cut(s) 289, 664, 810, 1009
BmrFI CCNGG 7 cut(s) 168, 213, 813, 964, 1243, 1412, 2092
BmrI ACTGGG 1 cut(s) 1166
BmsI GCATC 4 cut(s) 1028, 1452, 1865, 1938
BmuI ACTGGG 1 cut(s) 1166
BpiI GAAGAC 1 cut(s) 1460
BpmI CTGGAG 2 cut(s) 1065, 1277
Bpu1102I GCTNAGC 1 cut(s) 1952
BpuEI CTTGAG 3 cut(s) 644, 1446, 1474
BpuMI CCSGG 2 cut(s) 1243, 1412
BsaAI YACGTR 2 cut(s) 159, 223
BsaBI GATNNNNATC 1 cut(s) 159
BsaJI CCNNGG 3 cut(s) 812, 829, 1662
BsaXI ACNNNNNCTCC 4 cut(s) 766, 796, 1420, 1450
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse1I ACTGG 6 cut(s) 91, 418, 502, 681, 757, 1161
Bse3DI GCAATG 3 cut(s) 1743, 1819, 1963
Bse8I GATNNNNATC 1 cut(s) 159
BseBI CCWGG 5 cut(s) 168, 213, 813, 964, 2092
BseDI CCNNGG 3 cut(s) 812, 829, 1662
BseGI GGATG 5 cut(s) 571, 579, 761, 825, 1600
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMI GCAATG 3 cut(s) 1743, 1819, 1963
BseMII CTCAG 3 cut(s) 1290, 1644, 2094
BseNI ACTGG 6 cut(s) 91, 418, 502, 681, 757, 1161
BseRI GAGGAG 3 cut(s) 333, 1062, 1444
BseSI GKGCMC 1 cut(s) 1417
BseXI GCAGC 2 cut(s) 465, 1313
BsgI GTGCAG 1 cut(s) 1074
Bsh1236I CGCG 1 cut(s) 831
BshFI GGCC 6 cut(s) 192, 264, 437, 680, 1177, 1671
BshNI GGYRCC 1 cut(s) 287
BsiSI CCGG 2 cut(s) 1243, 1411
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 1 cut(s) 260
BsmAI GTCTC 3 cut(s) 1279, 1653, 1751
BsmFI GGGAC 1 cut(s) 365
BsnI GGCC 6 cut(s) 192, 264, 437, 680, 1177, 1671
Bsp1286I GDGCHC 2 cut(s) 1010, 1417
Bsp1720I GCTNAGC 1 cut(s) 1952
Bsp19I CCATGG 1 cut(s) 1662
BspACI CCGC 4 cut(s) 723, 829, 831, 967
BspANI GGCC 6 cut(s) 192, 264, 437, 680, 1177, 1671
BspCNI CTCAG 3 cut(s) 1289, 1645, 2093
BspFNI CGCG 1 cut(s) 831
BspLI GGNNCC 4 cut(s) 289, 664, 810, 1009
BspMI ACCTGC 1 cut(s) 1999
BspPI GGATC 5 cut(s) 657, 670, 1092, 1508, 1926
BspT107I GGYRCC 1 cut(s) 287
BspTI CTTAAG 1 cut(s) 101
BsrDI GCAATG 3 cut(s) 1743, 1819, 1963
BsrI ACTGG 6 cut(s) 91, 418, 502, 681, 757, 1161
BssECI CCNNGG 3 cut(s) 812, 829, 1662
BssNAI GTATAC 1 cut(s) 1349
BssSI CACGAG 1 cut(s) 258
BssT1I CCWWGG 1 cut(s) 1662
Bst1107I GTATAC 1 cut(s) 1349
Bst2BI CACGAG 1 cut(s) 258
Bst2UI CCWGG 5 cut(s) 168, 213, 813, 964, 2092
Bst6I CTCTTC 2 cut(s) 272, 1335
BstAFI CTTAAG 1 cut(s) 101
BstBAI YACGTR 2 cut(s) 159, 223
BstDEI CTNAG 5 cut(s) 597, 1276, 1653, 1952, 2080
BstDSI CCRYGG 2 cut(s) 829, 1662
BstEII GGTNACC 1 cut(s) 958
BstF5I GGATG 5 cut(s) 571, 579, 761, 825, 1600
BstFNI CGCG 1 cut(s) 831
BstHHI GCGC 1 cut(s) 1847
BstMAI GTCTC 3 cut(s) 1279, 1653, 1751
BstMWI GCNNNNNNNGC 1 cut(s) 16
BstNI CCWGG 5 cut(s) 168, 213, 813, 964, 2092
BstNSI RCATGY 3 cut(s) 1369, 1648, 2090
BstPI GGTNACC 1 cut(s) 958
BstSCI CCNGG 7 cut(s) 166, 211, 811, 962, 1241, 1410, 2090
BstSLI GKGCMC 1 cut(s) 1417
BstSNI TACGTA 1 cut(s) 159
BstUI CGCG 1 cut(s) 831
BstV1I GCAGC 2 cut(s) 465, 1313
BstV2I GAAGAC 1 cut(s) 1460
BstX2I RGATCY 1 cut(s) 662
BstYI RGATCY 1 cut(s) 662
BstZ17I GTATAC 1 cut(s) 1349
BsuRI GGCC 6 cut(s) 192, 264, 437, 680, 1177, 1671
BtgI CCRYGG 2 cut(s) 829, 1662
BtsCI GGATG 5 cut(s) 571, 579, 761, 825, 1600
BtsI GCAGTG 2 cut(s) 48, 1867
BtsIMutI CAGTG 6 cut(s) 48, 84, 495, 688, 1549, 1867
BveI ACCTGC 1 cut(s) 1999
CfoI GCGC 1 cut(s) 1847
Cfr13I GGNCC 4 cut(s) 97, 678, 809, 1175
Cfr42I CCGCGG 1 cut(s) 832
CseI GACGC 1 cut(s) 1888
Csp6I GTAC 5 cut(s) 120, 201, 1363, 1632, 1657
CviQI GTAC 5 cut(s) 120, 201, 1363, 1632, 1657
DdeI CTNAG 5 cut(s) 597, 1276, 1653, 1952, 2080
DraI TTTAAA 1 cut(s) 1518
Eam1104I CTCTTC 2 cut(s) 272, 1335
EarI CTCTTC 2 cut(s) 272, 1335
EciI GGCGGA 1 cut(s) 982
Eco105I TACGTA 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 1662
Eco147I AGGCCT 1 cut(s) 437
Eco24I GRGCYC 1 cut(s) 1010
Eco47I GGWCC 2 cut(s) 97, 809
Eco57I CTGAAG 1 cut(s) 717
Eco72I CACGTG 1 cut(s) 223
Eco91I GGTNACC 1 cut(s) 958
EcoO65I GGTNACC 1 cut(s) 958
EcoRII CCWGG 5 cut(s) 166, 211, 811, 962, 2090
EcoT14I CCWWGG 1 cut(s) 1662
EcoT22I ATGCAT 1 cut(s) 1880
EcoT38I GRGCYC 1 cut(s) 1010
ErhI CCWWGG 1 cut(s) 1662
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 1 cut(s) 365
FbaI TGATCA 3 cut(s) 982, 1710, 1762
FblI GTMKAC 2 cut(s) 940, 1348
Fnu4HI GCNGC 2 cut(s) 454, 1327
FokI GGATG 5 cut(s) 578, 586, 768, 812, 1607
FriOI GRGCYC 1 cut(s) 1010
Fsp4HI GCNGC 2 cut(s) 454, 1327
FspBI CTAG 2 cut(s) 1199, 1581
GlaI GCGC 1 cut(s) 1846
GluI GCNGC 2 cut(s) 454, 1327
GsuI CTGGAG 2 cut(s) 1065, 1277
HaeIII GGCC 6 cut(s) 192, 264, 437, 680, 1177, 1671
HapII CCGG 2 cut(s) 1243, 1411
HgaI GACGC 1 cut(s) 1888
HhaI GCGC 1 cut(s) 1847
Hin6I GCGC 1 cut(s) 1845
HinP1I GCGC 1 cut(s) 1845
HincII GTYRAC 2 cut(s) 761, 941
HindII GTYRAC 2 cut(s) 761, 941
HindIII AAGCTT 2 cut(s) 302, 1788
HinfI GANTC 6 cut(s) 547, 1400, 1618, 1832, 1961, 2002
HpaII CCGG 2 cut(s) 1243, 1411
HphI GGTGA 4 cut(s) 262, 640, 1499, 1720
Hpy166II GTNNAC 4 cut(s) 761, 779, 941, 1349
Hpy188I TCNGA 6 cut(s) 462, 741, 1567, 1690, 1936, 2083
Hpy188III TCNNGA 7 cut(s) 36, 80, 623, 1088, 1382, 1491, 1652
Hpy8I GTNNAC 4 cut(s) 761, 779, 941, 1349
Hpy99I CGWCG 1 cut(s) 945
HpyAV CCTTC 6 cut(s) 799, 1075, 1157, 1445, 1759, 1802
HpyCH4IV ACGT 4 cut(s) 158, 222, 781, 943
HpyF10VI GCNNNNNNNGC 1 cut(s) 16
HpyF3I CTNAG 5 cut(s) 597, 1276, 1653, 1952, 2080
HpySE526I ACGT 4 cut(s) 158, 222, 781, 943
HspAI GCGC 1 cut(s) 1845
Ksp22I TGATCA 3 cut(s) 982, 1710, 1762
KspI CCGCGG 1 cut(s) 832
LmnI GCTCC 3 cut(s) 326, 1013, 1296
Lsp1109I GCAGC 2 cut(s) 465, 1313
LweI GCATC 4 cut(s) 1028, 1452, 1865, 1938
MaeI CTAG 2 cut(s) 1199, 1581
MaeII ACGT 4 cut(s) 158, 222, 781, 943
MaeIII GTNAC 5 cut(s) 363, 602, 958, 1640, 2057
MfeI CAATTG 3 cut(s) 20, 709, 1965
MflI RGATCY 1 cut(s) 662
MhlI GDGCHC 2 cut(s) 1010, 1417
MlyI GAGTC 1 cut(s) 556
MmeI TCCRAC 2 cut(s) 504, 1518
Mph1103I ATGCAT 1 cut(s) 1880
MseI TTAA 6 cut(s) 102, 275, 296, 306, 1496, 1517
MslI CAYNNNNRTG 2 cut(s) 671, 1883
MspA1I CMGCKG 1 cut(s) 831
MspCI CTTAAG 1 cut(s) 101
MspI CCGG 2 cut(s) 1243, 1411
MspR9I CCNGG 7 cut(s) 168, 213, 813, 964, 1243, 1412, 2092
MunI CAATTG 3 cut(s) 20, 709, 1965
MvaI CCWGG 5 cut(s) 168, 213, 813, 964, 2092
MvnI CGCG 1 cut(s) 831
MwoI GCNNNNNNNGC 1 cut(s) 16
NciI CCSGG 2 cut(s) 1243, 1412
NcoI CCATGG 1 cut(s) 1662
NlaIV GGNNCC 4 cut(s) 289, 664, 810, 1009
NmuCI GTSAC 1 cut(s) 602
NsiI ATGCAT 1 cut(s) 1880
NspI RCATGY 3 cut(s) 1369, 1648, 2090
PceI AGGCCT 1 cut(s) 437
PciI ACATGT 1 cut(s) 1644
PfeI GAWTC 5 cut(s) 1400, 1618, 1832, 1961, 2002
PkrI GCNGC 2 cut(s) 455, 1328
PleI GAGTC 1 cut(s) 555
PmaCI CACGTG 1 cut(s) 223
PmlI CACGTG 1 cut(s) 223
PpsI GAGTC 1 cut(s) 555
Ppu21I YACGTR 2 cut(s) 159, 223
PscI ACATGT 1 cut(s) 1644
Psp6I CCWGG 5 cut(s) 166, 211, 811, 962, 2090
PspCI CACGTG 1 cut(s) 223
PspEI GGTNACC 1 cut(s) 958
PspGI CCWGG 5 cut(s) 166, 211, 811, 962, 2090
PspN4I GGNNCC 4 cut(s) 289, 664, 810, 1009
PspPI GGNCC 4 cut(s) 97, 678, 809, 1175
PsuI RGATCY 1 cut(s) 662
RsaI GTAC 5 cut(s) 121, 202, 1364, 1633, 1658
RsaNI GTAC 5 cut(s) 120, 201, 1363, 1632, 1657
RseI CAYNNNNRTG 2 cut(s) 671, 1883
SacII CCGCGG 1 cut(s) 832
SalI GTCGAC 1 cut(s) 939
SaqAI TTAA 6 cut(s) 102, 275, 296, 306, 1496, 1517
SatI GCNGC 2 cut(s) 454, 1327
Sau96I GGNCC 4 cut(s) 97, 678, 809, 1175
SchI GAGTC 1 cut(s) 556
ScrFI CCNGG 7 cut(s) 168, 213, 813, 964, 1243, 1412, 2092
SduI GDGCHC 2 cut(s) 1010, 1417
SfaNI GCATC 4 cut(s) 1028, 1452, 1865, 1938
Sfr303I CCGCGG 1 cut(s) 832
SgrBI CCGCGG 1 cut(s) 832
SinI GGWCC 2 cut(s) 97, 809
SmiMI CAYNNNNRTG 2 cut(s) 671, 1883
SmlI CTYRAG 4 cut(s) 101, 623, 1461, 1489
SmoI CTYRAG 4 cut(s) 101, 623, 1461, 1489
SnaBI TACGTA 1 cut(s) 159
SseBI AGGCCT 1 cut(s) 437
SsiI CCGC 4 cut(s) 723, 829, 831, 967
SspMI CTAG 2 cut(s) 1199, 1581
StuI AGGCCT 1 cut(s) 437
StyD4I CCNGG 7 cut(s) 166, 211, 811, 962, 1241, 1410, 2090
StyI CCWWGG 1 cut(s) 1662
TaiI ACGT 4 cut(s) 161, 225, 784, 946
TaqI TCGA 3 cut(s) 940, 1921, 1946
TatI WGTACW 2 cut(s) 200, 1362
TfiI GAWTC 5 cut(s) 1400, 1618, 1832, 1961, 2002
Tru1I TTAA 6 cut(s) 102, 275, 296, 306, 1496, 1517
Tru9I TTAA 6 cut(s) 102, 275, 296, 306, 1496, 1517
TscAI CASTG 6 cut(s) 55, 91, 502, 688, 1549, 1867
TseFI GTSAC 1 cut(s) 602
TseI GCWGC 2 cut(s) 453, 1326
Tsp45I GTSAC 1 cut(s) 602
TspDTI ATGAA 8 cut(s) 188, 759, 1053, 1284, 1381, 1469, 1565, 1974
TspGWI ACGGA 1 cut(s) 369
TspRI CASTG 6 cut(s) 55, 91, 502, 688, 1549, 1867
Vha464I CTTAAG 1 cut(s) 101
VpaK11BI GGWCC 2 cut(s) 97, 809
XapI RAATTY 7 cut(s) 91, 693, 1236, 1303, 1560, 1584, 2011
XceI RCATGY 3 cut(s) 1369, 1648, 2090
XcmI CCANNNNNNNNNTGG 2 cut(s) 1040, 1378
XmiI GTMKAC 2 cut(s) 940, 1348
XspI CTAG 2 cut(s) 1199, 1581
Zsp2I ATGCAT 1 cut(s) 1880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.