MD15G1048800.v1.1

Guanine nucleotide-binding protein alpha-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006871
Physical Location & Seq
Forward (+)
3319844 .. 3324162
4319 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1048800.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGCTGTCTATTTTGATCGAAAATATGGGATTACTCTGCAGCAGAAACAAGCATTACAACGAAGAGGACAATGAAGAGAACGCTCAGACTGCAGAAATTGAAAGGCGAATTGAACAAGAAACAAAGGCCGAAAAGCATATTCAGAAACTTCTCCTACTTGGTGCTGGGGAGTCAGGGAAGTCCACAATTTTTAAGCAGATAAAGCTTTTGTTTCAAACTGGATTTGAAGAGTCAGAGCTCAAGAGCTACATCTCAGTCATCCACGCCAACGTATATCAGACCATAAAAGTACTGTATGATGGGTCAAAGGAGCTTGCACAGAATGACAGAGAAACGTTTGAAATATCCATTGAAAATAAGGAAATTGGAGAGAAACTATCAACAATTGGCAGCAGATTGGATTATCCACGTCTCACTAAAGAGCTTGCACAGGATATAGAGACTCTTTGGAAAGATGCAGCAATTCAGGAAACATGTGCTCGTGGTAATGAACTCCAAGTTCCTGACTGTGCCCACTATTTCATGGAAAATCTTCAAAGATTAGCTGACCCAGATTACGTTCCAACCAAGGAGGATGTTCTTCATGCAAGAGTTCGTACAACTGGTGTCGTAGAGATCCAGTTCAGCCCTGTTGGAGAAAATAAGAGAAGTGGGGAAGTATATAGACTCTTTGACGTTGGGGGCCAGAGAAATGAGAGAAGGAAATGGATCCATCTATTTGAAGGCGTTACAGCCATAATATTTTGTGCTGCTCTTAGCGAGTATGATCAAACACTCTTTGAGGATGAAAGCAAGAATCGAATGATGGAGACAAGGGAACTTTTTGAGTGGGTTTTGAAGCAACCGTGTTTTGAGAAAACGTCGTTCATGCTGTTTTTAAACAAGTTTGATATATTCGAGAAGAAAGTTCTAAATGCGCCACTTAACGTCTGCGAGTGGTTCAAAGATTACCAGCCGGTTTCAACAGGAAAGCAAGAGATGGAACATGCATATGAGTATGTGAAGAAGAAATTTGAGGAATTGTACTTCCAGAGCACGGCCCCCGATCGCGTAGACCGGGTGTTTAAGATCTACAAAACCACCGCCCTTGATCAGAAGCTCGTGAAGAAGACTTTCAAGCTCGTGGACGAGACGTTGAGACGGAGAAATCTCTTCGAGGCCGGTTTATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0001101 GO:0001664 GO:0001789 GO:0001882 GO:0001883 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0003924 GO:0004857 GO:0005095 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005834 GO:0005886 GO:0005911 GO:0006082 GO:0006520 GO:0006558 GO:0006570 GO:0006571 GO:0006725 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009094 GO:0009095 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009637 GO:0009642 GO:0009645 GO:0009657 GO:0009668 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009739 GO:0009740 GO:0009741 GO:0009742 GO:0009743 GO:0009746 GO:0009749 GO:0009755 GO:0009785 GO:0009787 GO:0009788 GO:0009789 GO:0009791 GO:0009845 GO:0009870 GO:0009898 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010027 GO:0010033 GO:0010119 GO:0010244 GO:0010476 GO:0010646 GO:0010647 GO:0010648 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016053 GO:0016247 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0017144 GO:0019001 GO:0019438 GO:0019752 GO:0019897 GO:0019898 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030054 GO:0030234 GO:0030522 GO:0030695 GO:0031234 GO:0031347 GO:0031349 GO:0031683 GO:0031984 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032870 GO:0032991 GO:0033993 GO:0034260 GO:0034284 GO:0035639 GO:0036094 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0043086 GO:0043087 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043401 GO:0043436 GO:0044092 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0044877 GO:0045088 GO:0045089 GO:0045927 GO:0046394 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0048583 GO:0048584 GO:0048585 GO:0048638 GO:0048639 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051336 GO:0051346 GO:0051716 GO:0055044 GO:0060589 GO:0061024 GO:0065007 GO:0065009 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071367 GO:0071370 GO:0071383 GO:0071396 GO:0071407 GO:0071478 GO:0071482 GO:0071483 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0072593 GO:0080134 GO:0090351 GO:0097159 GO:0097305 GO:0097306 GO:0097367 GO:0098552 GO:0098562 GO:0098772 GO:0098796 GO:0098797 GO:0098827 GO:0104004 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901419 GO:1901420 GO:1901421 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901700 GO:1901701 GO:1902221 GO:1902223 GO:1902494 GO:1905360 GO:1905957 GO:1905958 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04742 ko04973 map04742 map04973
Pfam Domains
Protein Families
G-alpha

Protein Analysis

391

Amino Acids

45.63

Weight (kDa)

5.48

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-alpha PF00503 29 - 378 1.9e-108 G-protein alpha subunit
Arf PF00025 222 - 300 7.6e-07 ADP-ribosylation factor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1053
AccII CGCG 1 cut(s) 1050
AciI CCGC 1 cut(s) 1083
AclI AACGTT 1 cut(s) 335
AclWI GGATC 3 cut(s) 610, 703, 716
AcsI RAATTY 1 cut(s) 1010
AfaI GTAC 3 cut(s) 291, 598, 1025
AfiI CCNNNNNNNGG 1 cut(s) 1036
AflIII ACRYGT 1 cut(s) 473
AjiI CACGTC 1 cut(s) 410
AluBI AGCT 8 cut(s) 205, 238, 246, 313, 424, 545, 1099, 1120
AluI AGCT 8 cut(s) 205, 238, 246, 313, 424, 545, 1099, 1120
Alw21I GWGCWC 3 cut(s) 240, 481, 1037
Alw26I GTCTC 5 cut(s) 416, 434, 803, 1124, 1132
AlwI GGATC 3 cut(s) 610, 703, 716
AoxI GGCC 4 cut(s) 126, 682, 1038, 1158
ApeKI GCWGC 4 cut(s) 39, 390, 458, 749
ApoI RAATTY 1 cut(s) 1010
Asp700I GAANNNNTTC 2 cut(s) 531, 1112
AspLEI GCGC 1 cut(s) 919
AspS9I GGNCC 2 cut(s) 682, 1039
AsuC2I CCSGG 1 cut(s) 1058
BaeGI GKGCMC 1 cut(s) 514
BamHI GGATCC 1 cut(s) 708
BanII GRGCYC 1 cut(s) 240
BauI CACGAG 3 cut(s) 480, 1100, 1121
BbsI GAAGAC 1 cut(s) 1115
Bbv12I GWGCWC 3 cut(s) 240, 481, 1037
BbvI GCAGC 4 cut(s) 51, 402, 470, 736
BccI CCATC 4 cut(s) 293, 720, 799, 973
BceAI ACGGC 1 cut(s) 1053
BclI TGATCA 2 cut(s) 766, 1090
BcnI CCSGG 1 cut(s) 1058
BcoDI GTCTC 5 cut(s) 416, 434, 803, 1124, 1132
BfmI CTRYAG 2 cut(s) 37, 90
BglII AGATCT 1 cut(s) 1068
BisI GCNGC 4 cut(s) 40, 391, 459, 750
BlsI GCNGC 4 cut(s) 41, 392, 460, 751
BmcAI AGTACT 1 cut(s) 291
Bme1390I CCNGG 1 cut(s) 1058
BmgBI CACGTC 1 cut(s) 410
BmgT120I GGNCC 2 cut(s) 682, 1039
BmiI GGNNCC 3 cut(s) 683, 710, 1041
BmrFI CCNGG 1 cut(s) 1058
BmsI GCATC 1 cut(s) 445
BpiI GAAGAC 1 cut(s) 1115
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 1058
BsaJI CCNNGG 1 cut(s) 567
Bsc4I CCNNNNNNNGG 1 cut(s) 1036
Bse118I RCCGGY 2 cut(s) 955, 1160
Bse1I ACTGG 3 cut(s) 223, 607, 619
BseDI CCNNGG 1 cut(s) 567
BseGI GGATG 3 cut(s) 258, 580, 790
BseLI CCNNNNNNNGG 1 cut(s) 1036
BseMII CTCAG 2 cut(s) 98, 267
BseNI ACTGG 3 cut(s) 223, 607, 619
BseSI GKGCMC 1 cut(s) 514
BseXI GCAGC 4 cut(s) 51, 402, 470, 736
BseYI CCCAGC 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 1050
Bsh1285I CGRYCG 1 cut(s) 1048
BshFI GGCC 4 cut(s) 128, 684, 1040, 1160
BsiEI CGRYCG 1 cut(s) 1048
BsiHKAI GWGCWC 3 cut(s) 240, 481, 1037
BsiSI CCGG 3 cut(s) 956, 1057, 1161
BslI CCNNNNNNNGG 1 cut(s) 1036
BsmAI GTCTC 5 cut(s) 416, 434, 803, 1124, 1132
BsmBI CGTCTC 3 cut(s) 416, 1124, 1132
BsnI GGCC 4 cut(s) 128, 684, 1040, 1160
Bsp1286I GDGCHC 4 cut(s) 240, 481, 514, 1037
Bsp143I GATC 7 cut(s) 15, 615, 708, 766, 1045, 1068, 1090
BspACI CCGC 1 cut(s) 1083
BspANI GGCC 4 cut(s) 128, 684, 1040, 1160
BspCNI CTCAG 2 cut(s) 97, 266
BspFNI CGCG 1 cut(s) 1050
BspLI GGNNCC 3 cut(s) 683, 710, 1041
BspMAI CTGCAG 2 cut(s) 41, 94
BspPI GGATC 3 cut(s) 610, 703, 716
BsrFI RCCGGY 2 cut(s) 955, 1160
BsrI ACTGG 3 cut(s) 223, 607, 619
BssAI RCCGGY 2 cut(s) 955, 1160
BssECI CCNNGG 1 cut(s) 567
BssMI GATC 7 cut(s) 15, 615, 708, 766, 1045, 1068, 1090
BssSI CACGAG 3 cut(s) 480, 1100, 1121
BssT1I CCWWGG 1 cut(s) 567
Bst2BI CACGAG 3 cut(s) 480, 1100, 1121
Bst4CI ACNGT 3 cut(s) 294, 509, 846
Bst6I CTCTTC 4 cut(s) 57, 69, 222, 1157
BstC8I GCNNGC 2 cut(s) 315, 426
BstDEI CTNAG 3 cut(s) 84, 253, 755
BstF5I GGATG 3 cut(s) 258, 580, 790
BstFNI CGCG 1 cut(s) 1050
BstHHI GCGC 1 cut(s) 919
BstKTI GATC 7 cut(s) 18, 618, 711, 769, 1048, 1071, 1093
BstMAI GTCTC 5 cut(s) 416, 434, 803, 1124, 1132
BstMBI GATC 7 cut(s) 15, 615, 708, 766, 1045, 1068, 1090
BstMCI CGRYCG 1 cut(s) 1048
BstMWI GCNNNNNNNGC 2 cut(s) 89, 202
BstNSI RCATGY 2 cut(s) 477, 989
BstSCI CCNGG 1 cut(s) 1056
BstSFI CTRYAG 2 cut(s) 37, 90
BstSLI GKGCMC 1 cut(s) 514
BstUI CGCG 1 cut(s) 1050
BstV1I GCAGC 4 cut(s) 51, 402, 470, 736
BstV2I GAAGAC 1 cut(s) 1115
BstX2I RGATCY 3 cut(s) 615, 708, 1068
BstYI RGATCY 3 cut(s) 615, 708, 1068
BsuRI GGCC 4 cut(s) 128, 684, 1040, 1160
BtrI CACGTC 1 cut(s) 410
BtsCI GGATG 3 cut(s) 258, 580, 790
Cac8I GCNNGC 2 cut(s) 315, 426
CfoI GCGC 1 cut(s) 919
Cfr10I RCCGGY 2 cut(s) 955, 1160
Cfr13I GGNCC 2 cut(s) 682, 1039
Csp6I GTAC 3 cut(s) 290, 597, 1024
CspCI CAANNNNNGTGG 2 cut(s) 1069, 1104
CviAII CATG 5 cut(s) 474, 523, 584, 868, 986
CviQI GTAC 3 cut(s) 290, 597, 1024
DdeI CTNAG 3 cut(s) 84, 253, 755
DpnI GATC 7 cut(s) 17, 617, 710, 768, 1047, 1070, 1092
DpnII GATC 7 cut(s) 15, 615, 708, 766, 1045, 1068, 1090
DraI TTTAAA 1 cut(s) 879
Eam1104I CTCTTC 4 cut(s) 57, 69, 222, 1157
EarI CTCTTC 4 cut(s) 57, 69, 222, 1157
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 567
Eco24I GRGCYC 1 cut(s) 240
Eco53kI GAGCTC 1 cut(s) 238
EcoICRI GAGCTC 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 567
EcoT22I ATGCAT 1 cut(s) 991
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 567
Esp3I CGTCTC 3 cut(s) 416, 1124, 1132
FaeI CATG 5 cut(s) 477, 526, 587, 871, 989
FatI CATG 5 cut(s) 473, 522, 583, 867, 985
FauNDI CATATG 1 cut(s) 991
FbaI TGATCA 2 cut(s) 766, 1090
FblI GTMKAC 1 cut(s) 1053
Fnu4HI GCNGC 4 cut(s) 40, 391, 459, 750
FokI GGATG 3 cut(s) 245, 587, 797
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 4 cut(s) 40, 391, 459, 750
GlaI GCGC 1 cut(s) 918
GluI GCNGC 4 cut(s) 40, 391, 459, 750
GsaI CCCAGC 1 cut(s) 168
HaeIII GGCC 4 cut(s) 128, 684, 1040, 1160
HapII CCGG 3 cut(s) 956, 1057, 1161
HhaI GCGC 1 cut(s) 919
Hin1II CATG 5 cut(s) 477, 526, 587, 871, 989
Hin6I GCGC 1 cut(s) 917
HinP1I GCGC 1 cut(s) 917
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 5 cut(s) 170, 230, 442, 666, 796
HpaII CCGG 3 cut(s) 956, 1057, 1161
Hpy166II GTNNAC 3 cut(s) 183, 1054, 1126
Hpy188I TCNGA 5 cut(s) 87, 144, 235, 279, 1095
Hpy188III TCNNGA 6 cut(s) 241, 467, 503, 898, 1030, 1102
Hpy8I GTNNAC 3 cut(s) 183, 1054, 1126
Hpy99I CGWCG 1 cut(s) 865
HpyAV CCTTC 2 cut(s) 693, 716
HpyCH4III ACNGT 3 cut(s) 294, 509, 846
HpyCH4IV ACGT 8 cut(s) 270, 335, 409, 558, 675, 860, 927, 1133
HpyCH4V TGCA 7 cut(s) 39, 92, 317, 428, 458, 587, 989
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 202
HpyF3I CTNAG 3 cut(s) 84, 253, 755
HpySE526I ACGT 8 cut(s) 270, 335, 409, 558, 675, 860, 927, 1133
Hsp92II CATG 5 cut(s) 477, 526, 587, 871, 989
HspAI GCGC 1 cut(s) 917
Ksp22I TGATCA 2 cut(s) 766, 1090
Kzo9I GATC 7 cut(s) 15, 615, 708, 766, 1045, 1068, 1090
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 4 cut(s) 51, 402, 470, 736
LweI GCATC 1 cut(s) 445
MaeII ACGT 8 cut(s) 270, 335, 409, 558, 675, 860, 927, 1133
MaeIII GTNAC 1 cut(s) 727
MalI GATC 7 cut(s) 17, 617, 710, 768, 1047, 1070, 1092
MboI GATC 7 cut(s) 15, 615, 708, 766, 1045, 1068, 1090
MfeI CAATTG 1 cut(s) 384
MflI RGATCY 3 cut(s) 615, 708, 1068
MhlI GDGCHC 4 cut(s) 240, 481, 514, 1037
MluCI AATT 8 cut(s) 96, 108, 186, 363, 384, 462, 1010, 1019
MlyI GAGTC 4 cut(s) 179, 239, 436, 660
MmeI TCCRAC 2 cut(s) 587, 613
MnlI CCTC 5 cut(s) 58, 565, 775, 1009, 1150
Mph1103I ATGCAT 1 cut(s) 991
MroXI GAANNNNTTC 2 cut(s) 531, 1112
MseI TTAA 4 cut(s) 192, 878, 924, 1065
MslI CAYNNNNRTG 1 cut(s) 990
MspI CCGG 3 cut(s) 956, 1057, 1161
MspR9I CCNGG 1 cut(s) 1058
MunI CAATTG 1 cut(s) 384
MvnI CGCG 1 cut(s) 1050
MwoI GCNNNNNNNGC 2 cut(s) 89, 202
NciI CCSGG 1 cut(s) 1058
NdeI CATATG 1 cut(s) 991
NdeII GATC 7 cut(s) 15, 615, 708, 766, 1045, 1068, 1090
NlaIII CATG 5 cut(s) 477, 526, 587, 871, 989
NlaIV GGNNCC 3 cut(s) 683, 710, 1041
NsiI ATGCAT 1 cut(s) 991
NspI RCATGY 2 cut(s) 477, 989
PciI ACATGT 1 cut(s) 473
PdmI GAANNNNTTC 2 cut(s) 531, 1112
PfeI GAWTC 1 cut(s) 796
PkrI GCNGC 4 cut(s) 41, 392, 460, 751
Ple19I CGATCG 1 cut(s) 1048
PleI GAGTC 4 cut(s) 178, 238, 436, 660
PpsI GAGTC 4 cut(s) 178, 238, 436, 660
PscI ACATGT 1 cut(s) 473
Psp124BI GAGCTC 1 cut(s) 240
Psp1406I AACGTT 1 cut(s) 335
PspFI CCCAGC 1 cut(s) 164
PspN4I GGNNCC 3 cut(s) 683, 710, 1041
PspPI GGNCC 2 cut(s) 682, 1039
PstI CTGCAG 2 cut(s) 41, 94
PsuI RGATCY 3 cut(s) 615, 708, 1068
PvuI CGATCG 1 cut(s) 1048
RsaI GTAC 3 cut(s) 291, 598, 1025
RsaNI GTAC 3 cut(s) 290, 597, 1024
RseI CAYNNNNRTG 1 cut(s) 990
SacI GAGCTC 1 cut(s) 240
SaqAI TTAA 4 cut(s) 192, 878, 924, 1065
SatI GCNGC 4 cut(s) 40, 391, 459, 750
Sau3AI GATC 7 cut(s) 15, 615, 708, 766, 1045, 1068, 1090
Sau96I GGNCC 2 cut(s) 682, 1039
ScaI AGTACT 1 cut(s) 291
SchI GAGTC 4 cut(s) 179, 239, 436, 660
ScrFI CCNGG 1 cut(s) 1058
SduI GDGCHC 4 cut(s) 240, 481, 514, 1037
SfaNI GCATC 1 cut(s) 445
SfcI CTRYAG 2 cut(s) 37, 90
SmiMI CAYNNNNRTG 1 cut(s) 990
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 8 cut(s) 96, 108, 186, 363, 384, 462, 1010, 1019
SsiI CCGC 1 cut(s) 1083
SspI AATATT 1 cut(s) 741
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 1 cut(s) 1056
StyI CCWWGG 1 cut(s) 567
TaaI ACNGT 3 cut(s) 294, 509, 846
TaiI ACGT 8 cut(s) 273, 338, 412, 561, 678, 863, 930, 1136
TaqI TCGA 4 cut(s) 18, 799, 897, 1155
TasI AATT 8 cut(s) 96, 108, 186, 363, 384, 462, 1010, 1019
TatI WGTACW 2 cut(s) 289, 1023
TfiI GAWTC 1 cut(s) 796
Tru1I TTAA 4 cut(s) 192, 878, 924, 1065
Tru9I TTAA 4 cut(s) 192, 878, 924, 1065
TseI GCWGC 4 cut(s) 39, 390, 458, 749
TspDTI ATGAA 6 cut(s) 87, 504, 511, 572, 801, 856
TspGWI ACGGA 1 cut(s) 1156
XapI RAATTY 1 cut(s) 1010
XceI RCATGY 2 cut(s) 477, 989
XmiI GTMKAC 1 cut(s) 1053
XmnI GAANNNNTTC 2 cut(s) 531, 1112
ZrmI AGTACT 1 cut(s) 291
Zsp2I ATGCAT 1 cut(s) 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.