RLG00000010363

Guanine nucleotide-binding protein alpha-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006871
Physical Location & Seq
Forward (+)
999099 .. 1003490
4392 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010363

Sequence Viewer

Length: 1173 bp
ATGCTGTCTATTGTGTTAGAAAATATGGGCTCACTCTGCAGCAAAAACCAGCACTACAATGAAGCGGACAATGAAGAGAATGCACAGACTGCAGAAATTGAAAGGAGAATTGAACAAGAAACGAAGGCTGAAAAACATATCCAGAAACTTCTCCTACTAGGTGCTGGGGAGTCAGGGAAGTCCACAATCTTCAAGCAGATAAAGCTTTTGTTTCAAACCGGCTTCGATGATGCAGAACTCAAGAGCTACATATCAGTCATACATGCCAATGTGTATCAGACCATAAAAATATTGTATGATGGGTCAAAGGAGCTCGCTCAGAATGACAGTGAAACATATGAAATATCTAGTGAAAATCAGGAAATTGGAGAGAAACTATCAAAAATAGGGGGCAGATTGGATTATCCACGTCTAACTAAAGAGCTTGCACAGGAGATAGAGACTCTTTGGAAAGATGCTGCAATTCAGGAAACATATACTCGTGGTAATGAACTTCAAGTTCCTGATTGTGCCAACTATTTCATGGAAAATCTACACAGATTAGCTGACACAAATTACATTCCAACCAAGGAAGATGTTCTTTATGCAAGAGTTCGTACAACTGGTGTTGTAGAGATCCAGTTTAGCCCTGTTGGCGAAAACAAGAAAAGTGGTGAAGTATATAGACTCTTTGATGTTGGAGGCCAGAGAAATGAGAGGAGGAAATGGATCCATCTTTTTGAAGGTGTTACAGCTGTTATATTTTGTGCTGCTGTTAGCGATTATGATCAAACACTCTTTGAGGATGAAAGCAAGAATCGAATGATGGAAACAAAGGAACTTTTTGACTGGGTCCTGAAGCAACCTTGTTTTGAGAAAACGTCCTTCATGCTATTTCTAAACAAGTTTGATATATTCGAGAAGAAGGTTCTAAAGGTGCCACTTAATGTGTGCGAGTGGTTCAAAGATTACCAGCCAGTTTCTACAGGAAAACAAGAAATTGAACATGCATACGAGTTTGTGAAGAAAAAATTTGAGGAATTGTATTTTCAGAGCACCACCCCGGATCGTGTGGACCGCGTGTTTAAGATCTATAGAACAACTGCCCTTGATCAGAAGTTGGTGAAGAAGACTTTCAAGCTTGTGGATGAGACCTTAAGACGGAGAAATCTGTTCGAGGCAGGTTTGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0001101 GO:0001664 GO:0001789 GO:0001882 GO:0001883 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0003924 GO:0004857 GO:0005095 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005834 GO:0005886 GO:0005911 GO:0006082 GO:0006520 GO:0006558 GO:0006570 GO:0006571 GO:0006725 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009094 GO:0009095 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009637 GO:0009642 GO:0009645 GO:0009657 GO:0009668 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009739 GO:0009740 GO:0009741 GO:0009742 GO:0009743 GO:0009746 GO:0009749 GO:0009755 GO:0009785 GO:0009787 GO:0009788 GO:0009789 GO:0009791 GO:0009845 GO:0009870 GO:0009898 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010027 GO:0010033 GO:0010119 GO:0010244 GO:0010476 GO:0010646 GO:0010647 GO:0010648 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016053 GO:0016247 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0017144 GO:0019001 GO:0019438 GO:0019752 GO:0019897 GO:0019898 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030054 GO:0030234 GO:0030522 GO:0030695 GO:0031234 GO:0031347 GO:0031349 GO:0031683 GO:0031984 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032870 GO:0032991 GO:0033993 GO:0034260 GO:0034284 GO:0035639 GO:0036094 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0043086 GO:0043087 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043401 GO:0043436 GO:0044092 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0044877 GO:0045088 GO:0045089 GO:0045927 GO:0046394 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0048583 GO:0048584 GO:0048585 GO:0048638 GO:0048639 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051336 GO:0051346 GO:0051716 GO:0055044 GO:0060589 GO:0061024 GO:0065007 GO:0065009 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071367 GO:0071370 GO:0071383 GO:0071396 GO:0071407 GO:0071478 GO:0071482 GO:0071483 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0072593 GO:0080134 GO:0090351 GO:0097159 GO:0097305 GO:0097306 GO:0097367 GO:0098552 GO:0098562 GO:0098772 GO:0098796 GO:0098797 GO:0098827 GO:0104004 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901419 GO:1901420 GO:1901421 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901700 GO:1901701 GO:1902221 GO:1902223 GO:1902494 GO:1905360 GO:1905957 GO:1905958 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04742 ko04973 map04742 map04973
Pfam Domains
Protein Families
G-alpha

Protein Analysis

391

Amino Acids

45.48

Weight (kDa)

5.48

Isoelectric Point (pI)

35.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-alpha PF00503 29 - 378 5.5e-107 G-protein alpha subunit
Arf PF00025 221 - 300 1.4e-07 ADP-ribosylation factor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1151
AccB1I GGYRCC 1 cut(s) 916
AccII CGCG 1 cut(s) 1059
AciI CCGC 2 cut(s) 65, 1057
AclWI GGATC 4 cut(s) 610, 703, 716, 1053
AcsI RAATTY 1 cut(s) 1010
AcuI CTGAAG 1 cut(s) 857
AfaI GTAC 1 cut(s) 598
AfiI CCNNNNNNNGG 1 cut(s) 1140
AflII CTTAAG 1 cut(s) 1135
AgsI TTSAA 9 cut(s) 101, 113, 193, 215, 497, 722, 943, 983, 1117
AjiI CACGTC 1 cut(s) 410
AluBI AGCT 7 cut(s) 205, 246, 313, 424, 545, 734, 1120
AluI AGCT 7 cut(s) 205, 246, 313, 424, 545, 734, 1120
Alw21I GWGCWC 2 cut(s) 315, 1037
Alw26I GTCTC 2 cut(s) 434, 1124
AlwI GGATC 4 cut(s) 610, 703, 716, 1053
AoxI GGCC 1 cut(s) 682
ApeKI GCWGC 3 cut(s) 39, 458, 749
ApoI RAATTY 1 cut(s) 1010
Asp700I GAANNNNTTC 2 cut(s) 576, 1112
AspS9I GGNCC 2 cut(s) 832, 1054
AsuC2I CCSGG 1 cut(s) 1043
AsuHPI GGTGA 2 cut(s) 665, 1114
AvaII GGWCC 2 cut(s) 832, 1054
BamHI GGATCC 1 cut(s) 708
BanI GGYRCC 1 cut(s) 916
BanII GRGCYC 2 cut(s) 32, 315
BauI CACGAG 1 cut(s) 480
BbsI GAAGAC 1 cut(s) 1115
Bbv12I GWGCWC 2 cut(s) 315, 1037
BbvI GCAGC 3 cut(s) 51, 445, 736
BccI CCATC 3 cut(s) 293, 720, 799
BclI TGATCA 2 cut(s) 766, 1090
BcnI CCSGG 1 cut(s) 1043
BcoDI GTCTC 2 cut(s) 434, 1124
BfaI CTAG 2 cut(s) 158, 348
BfmI CTRYAG 4 cut(s) 37, 90, 963, 1072
BfrI CTTAAG 1 cut(s) 1135
BfuAI ACCTGC 1 cut(s) 1151
BglI GCCNNNNNGGC 1 cut(s) 633
BglII AGATCT 1 cut(s) 1068
BisI GCNGC 3 cut(s) 40, 459, 750
BlsI GCNGC 3 cut(s) 41, 460, 751
Bme1390I CCNGG 1 cut(s) 1043
Bme18I GGWCC 2 cut(s) 832, 1054
BmgBI CACGTC 1 cut(s) 410
BmgT120I GGNCC 2 cut(s) 832, 1054
BmiI GGNNCC 3 cut(s) 710, 833, 918
BmrFI CCNGG 1 cut(s) 1043
BmrI ACTGGG 1 cut(s) 838
BmsI GCATC 2 cut(s) 220, 445
BmuI ACTGGG 1 cut(s) 838
BpiI GAAGAC 1 cut(s) 1115
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 1043
BsaBI GATNNNNATC 1 cut(s) 765
BsaI GGTCTC 1 cut(s) 1124
BsaJI CCNNGG 2 cut(s) 567, 1041
Bsc4I CCNNNNNNNGG 1 cut(s) 1140
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 4 cut(s) 607, 619, 833, 956
Bse8I GATNNNNATC 1 cut(s) 765
BseDI CCNNGG 2 cut(s) 567, 1041
BseGI GGATG 2 cut(s) 790, 1132
BseJI GATNNNNATC 1 cut(s) 765
BseLI CCNNNNNNNGG 1 cut(s) 1140
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 4 cut(s) 607, 619, 833, 956
BseRI GAGGAG 1 cut(s) 712
BseXI GCAGC 3 cut(s) 51, 445, 736
BseYI CCCAGC 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 1059
BshFI GGCC 1 cut(s) 684
BshNI GGYRCC 1 cut(s) 916
BsiHKAI GWGCWC 2 cut(s) 315, 1037
BsiSI CCGG 2 cut(s) 219, 1043
BslI CCNNNNNNNGG 1 cut(s) 1140
BsmAI GTCTC 2 cut(s) 434, 1124
BsmI GAATGC 1 cut(s) 85
BsnI GGCC 1 cut(s) 684
Bso31I GGTCTC 1 cut(s) 1124
Bsp1286I GDGCHC 3 cut(s) 32, 315, 1037
Bsp143I GATC 6 cut(s) 615, 708, 766, 1045, 1068, 1090
BspACI CCGC 2 cut(s) 65, 1057
BspANI GGCC 1 cut(s) 684
BspCNI CTCAG 1 cut(s) 331
BspFNI CGCG 1 cut(s) 1059
BspLI GGNNCC 3 cut(s) 710, 833, 918
BspMAI CTGCAG 2 cut(s) 41, 94
BspMI ACCTGC 1 cut(s) 1151
BspPI GGATC 4 cut(s) 610, 703, 716, 1053
BspT107I GGYRCC 1 cut(s) 916
BspTI CTTAAG 1 cut(s) 1135
BspTNI GGTCTC 1 cut(s) 1124
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 4 cut(s) 607, 619, 833, 956
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 2 cut(s) 567, 1041
BssMI GATC 6 cut(s) 615, 708, 766, 1045, 1068, 1090
BssSI CACGAG 1 cut(s) 480
BssT1I CCWWGG 1 cut(s) 567
Bst2BI CACGAG 1 cut(s) 480
Bst4CI ACNGT 1 cut(s) 329
Bst6I CTCTTC 1 cut(s) 69
BstAFI CTTAAG 1 cut(s) 1135
BstAPI GCANNNNNTGC 1 cut(s) 89
BstC8I GCNNGC 2 cut(s) 315, 426
BstDEI CTNAG 1 cut(s) 318
BstF5I GGATG 2 cut(s) 790, 1132
BstFNI CGCG 1 cut(s) 1059
BstKTI GATC 6 cut(s) 618, 711, 769, 1048, 1071, 1093
BstMAI GTCTC 2 cut(s) 434, 1124
BstMBI GATC 6 cut(s) 615, 708, 766, 1045, 1068, 1090
BstMWI GCNNNNNNNGC 4 cut(s) 36, 89, 202, 633
BstNSI RCATGY 2 cut(s) 266, 989
BstSCI CCNGG 1 cut(s) 1041
BstSFI CTRYAG 4 cut(s) 37, 90, 963, 1072
BstUI CGCG 1 cut(s) 1059
BstV1I GCAGC 3 cut(s) 51, 445, 736
BstV2I GAAGAC 1 cut(s) 1115
BstX2I RGATCY 3 cut(s) 615, 708, 1068
BstYI RGATCY 3 cut(s) 615, 708, 1068
BsuRI GGCC 1 cut(s) 684
BtrI CACGTC 1 cut(s) 410
BtsCI GGATG 2 cut(s) 790, 1132
BtsIMutI CAGTG 1 cut(s) 334
BveI ACCTGC 1 cut(s) 1151
Cac8I GCNNGC 2 cut(s) 315, 426
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 2 cut(s) 832, 1054
Csp6I GTAC 1 cut(s) 597
CspCI CAANNNNNGTGG 2 cut(s) 631, 666
CviAII CATG 4 cut(s) 263, 523, 868, 986
CviQI GTAC 1 cut(s) 597
DdeI CTNAG 1 cut(s) 318
DpnI GATC 6 cut(s) 617, 710, 768, 1047, 1070, 1092
DpnII GATC 6 cut(s) 615, 708, 766, 1045, 1068, 1090
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Ecl136II GAGCTC 1 cut(s) 313
Eco130I CCWWGG 1 cut(s) 567
Eco24I GRGCYC 2 cut(s) 32, 315
Eco31I GGTCTC 1 cut(s) 1124
Eco47I GGWCC 2 cut(s) 832, 1054
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 857
EcoICRI GAGCTC 1 cut(s) 313
EcoO109I RGGNCCY 1 cut(s) 832
EcoT14I CCWWGG 1 cut(s) 567
EcoT22I ATGCAT 1 cut(s) 991
EcoT38I GRGCYC 2 cut(s) 32, 315
ErhI CCWWGG 1 cut(s) 567
FaeI CATG 4 cut(s) 266, 526, 871, 989
FalI AAGNNNNNCTT 2 cut(s) 564, 596
FatI CATG 4 cut(s) 262, 522, 867, 985
FauNDI CATATG 1 cut(s) 337
FbaI TGATCA 2 cut(s) 766, 1090
Fnu4HI GCNGC 3 cut(s) 40, 459, 750
FokI GGATG 2 cut(s) 797, 1139
FriOI GRGCYC 2 cut(s) 32, 315
Fsp4HI GCNGC 3 cut(s) 40, 459, 750
FspBI CTAG 2 cut(s) 158, 348
GluI GCNGC 3 cut(s) 40, 459, 750
GsaI CCCAGC 1 cut(s) 168
HaeIII GGCC 1 cut(s) 684
HapII CCGG 2 cut(s) 219, 1043
Hin1II CATG 4 cut(s) 266, 526, 871, 989
HindIII AAGCTT 2 cut(s) 203, 1118
HinfI GANTC 4 cut(s) 170, 442, 666, 796
HpaII CCGG 2 cut(s) 219, 1043
HphI GGTGA 2 cut(s) 665, 1114
Hpy166II GTNNAC 2 cut(s) 183, 1054
Hpy188I TCNGA 4 cut(s) 279, 321, 1032, 1095
Hpy188III TCNNGA 7 cut(s) 142, 241, 359, 467, 503, 835, 898
Hpy8I GTNNAC 2 cut(s) 183, 1054
HpyAV CCTTC 4 cut(s) 118, 716, 874, 898
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4IV ACGT 2 cut(s) 409, 860
HpyCH4V TGCA 8 cut(s) 39, 83, 92, 233, 428, 461, 587, 989
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 89, 202, 633
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 2 cut(s) 409, 860
Hsp92II CATG 4 cut(s) 266, 526, 871, 989
Ksp22I TGATCA 2 cut(s) 766, 1090
Kzo9I GATC 6 cut(s) 615, 708, 766, 1045, 1068, 1090
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 3 cut(s) 51, 445, 736
LweI GCATC 2 cut(s) 220, 445
MaeI CTAG 2 cut(s) 158, 348
MaeII ACGT 2 cut(s) 409, 860
MaeIII GTNAC 1 cut(s) 727
MalI GATC 6 cut(s) 617, 710, 768, 1047, 1070, 1092
MboI GATC 6 cut(s) 615, 708, 766, 1045, 1068, 1090
MboII GAAGA 7 cut(s) 86, 181, 584, 913, 1015, 1117, 1120
MflI RGATCY 3 cut(s) 615, 708, 1068
MhlI GDGCHC 3 cut(s) 32, 315, 1037
MluCI AATT 8 cut(s) 96, 108, 363, 462, 553, 978, 1010, 1019
MlyI GAGTC 3 cut(s) 179, 436, 660
MmeI TCCRAC 2 cut(s) 587, 658
MnlI CCTC 6 cut(s) 674, 690, 693, 775, 1009, 1150
Mph1103I ATGCAT 1 cut(s) 991
MroXI GAANNNNTTC 2 cut(s) 576, 1112
MseI TTAA 3 cut(s) 924, 1065, 1136
MslI CAYNNNNRTG 2 cut(s) 57, 267
MspA1I CMGCKG 1 cut(s) 734
MspCI CTTAAG 1 cut(s) 1135
MspI CCGG 2 cut(s) 219, 1043
MspR9I CCNGG 1 cut(s) 1043
Mva1269I GAATGC 1 cut(s) 85
MvnI CGCG 1 cut(s) 1059
MwoI GCNNNNNNNGC 4 cut(s) 36, 89, 202, 633
NciI CCSGG 1 cut(s) 1043
NdeI CATATG 1 cut(s) 337
NdeII GATC 6 cut(s) 615, 708, 766, 1045, 1068, 1090
NlaIII CATG 4 cut(s) 266, 526, 871, 989
NlaIV GGNNCC 3 cut(s) 710, 833, 918
NsiI ATGCAT 1 cut(s) 991
NspI RCATGY 2 cut(s) 266, 989
PctI GAATGC 1 cut(s) 85
PdmI GAANNNNTTC 2 cut(s) 576, 1112
PfeI GAWTC 1 cut(s) 796
PflFI GACNNNGTC 1 cut(s) 830
PkrI GCNGC 3 cut(s) 41, 460, 751
PleI GAGTC 3 cut(s) 178, 436, 660
PpsI GAGTC 3 cut(s) 178, 436, 660
PpuMI RGGWCCY 1 cut(s) 832
Psp124BI GAGCTC 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 832
PspFI CCCAGC 1 cut(s) 164
PspN4I GGNNCC 3 cut(s) 710, 833, 918
PspPI GGNCC 2 cut(s) 832, 1054
PspPPI RGGWCCY 1 cut(s) 832
PstI CTGCAG 2 cut(s) 41, 94
PsuI RGATCY 3 cut(s) 615, 708, 1068
PsyI GACNNNGTC 1 cut(s) 830
PvuII CAGCTG 1 cut(s) 734
RsaI GTAC 1 cut(s) 598
RsaNI GTAC 1 cut(s) 597
RseI CAYNNNNRTG 2 cut(s) 57, 267
SacI GAGCTC 1 cut(s) 315
SaqAI TTAA 3 cut(s) 924, 1065, 1136
SatI GCNGC 3 cut(s) 40, 459, 750
Sau3AI GATC 6 cut(s) 615, 708, 766, 1045, 1068, 1090
Sau96I GGNCC 2 cut(s) 832, 1054
SchI GAGTC 3 cut(s) 179, 436, 660
ScrFI CCNGG 1 cut(s) 1043
SduI GDGCHC 3 cut(s) 32, 315, 1037
SfaNI GCATC 2 cut(s) 220, 445
SfcI CTRYAG 4 cut(s) 37, 90, 963, 1072
SinI GGWCC 2 cut(s) 832, 1054
SmiMI CAYNNNNRTG 2 cut(s) 57, 267
SmlI CTYRAG 2 cut(s) 239, 1135
SmoI CTYRAG 2 cut(s) 239, 1135
Sse9I AATT 8 cut(s) 96, 108, 363, 462, 553, 978, 1010, 1019
SsiI CCGC 2 cut(s) 65, 1057
SspI AATATT 1 cut(s) 291
SspMI CTAG 2 cut(s) 158, 348
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 1 cut(s) 1041
StyI CCWWGG 1 cut(s) 567
TaaI ACNGT 1 cut(s) 329
TaiI ACGT 2 cut(s) 412, 863
TaqI TCGA 4 cut(s) 225, 799, 897, 1155
TasI AATT 8 cut(s) 96, 108, 363, 462, 553, 978, 1010, 1019
TfiI GAWTC 1 cut(s) 796
Tru1I TTAA 3 cut(s) 924, 1065, 1136
Tru9I TTAA 3 cut(s) 924, 1065, 1136
TscAI CASTG 1 cut(s) 334
TseI GCWGC 3 cut(s) 39, 458, 749
TspDTI ATGAA 7 cut(s) 75, 87, 354, 504, 511, 801, 856
TspGWI ACGGA 1 cut(s) 1156
TspRI CASTG 1 cut(s) 334
Tth111I GACNNNGTC 1 cut(s) 830
Vha464I CTTAAG 1 cut(s) 1135
VpaK11BI GGWCC 2 cut(s) 832, 1054
XapI RAATTY 1 cut(s) 1010
XceI RCATGY 2 cut(s) 266, 989
XcmI CCANNNNNNNNNTGG 1 cut(s) 520
XmnI GAANNNNTTC 2 cut(s) 576, 1112
XspI CTAG 2 cut(s) 158, 348
Zsp2I ATGCAT 1 cut(s) 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.