MD08G1081000.v1.1

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015557
Physical Location & Seq
Forward (+)
6739399 .. 6742423
3025 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1081000.v1.1.491

Sequence Viewer

Length: 2169 bp
ATGGCGTCTAATTACAGGTCAGCCGATTCGAGGCTCTCCGATCTCGAGCTAGCATGTTCCGATTTGAAAACTCTCCTAACAGCGACGGCGAAAATGGAGGAAAATCTTCAACAGATGGACAACAAGTTCGAAGTCATAGACGAAACGCTTCTAACTGCGTCTAAAAGAGTAGCTCCTATACAATCACTAGCCATGGCCTCGAAAGCCTTAGAAACCAGAATCAACCGAGCTGTCACGCCGGCCCTGGCGCTTCTCGACAATTTCAAGCTCTCCGAGACCATCCAGGACAAGCTCTTGGATCTCGCGGAGACGTTGTCCAGCGAGAAGTCAAGGAAGAAACGGCTCAAGAAGCTTGTCAAGTACGTGGACTGCGTGGATCAGTTGAAAATTGCGATTAGCTGTATTTGCGAAGAAGGTGAGCCAGTGATTCAGAGGCTGCAAGAGGTGGTGGAGTTTTTGAGCCGGACGAAGGCGACCGACCAGTACAGGACTCACCGGCTGAGGGAGACGCTGGTGACTCTCAAGGCGCTGTACGAGACGGAGGTGGACGCCATGAGGTTTGAAGGGTTGCTTGATGACGCGCTGCTGAATTTGCAGGACGAGTATGAGAGTATACTGGAGCAGATTAAACATCGGAATGTGGTGGATTTGCAGGCTGCTTCGGATAAAGATGACCGGAGGAATGAGATAGCGATCGTGGGTTCGGATTTGGGGACTGAGTTGGAGGTTGAACTTCTCAGAAGGATTTCACAAACCCTAGCTGCCGATGATTGCTTGGACATATGCATCGATATTTATGTTAAGGTGAGGTACAAAAGAGTGGCCAAAGCACTGATGAGGCTAAACCCGGATTACCTAAAAACACACACACCAGAAGAAATAGATGAAATGCCATGGGAGAGCTTGGAGACAGCCATAACCCTTTGGATCCAGCACTTCGAGCTCGCAGTCAAAGCAGTTCTTGTATCGGAGAAGAAGCTCTGCGAACAAGTCCTAGGAGGAATAATGGAGGGACTGATATGGCCAGAATGCTTCGCCAAAATCGCAGACAAAATCATGGCAGTGTTCTTCAGGTTCGGTGAAGCCGTTGCCCGGAGTAGCAAAGAGCCCCAGAAGCTGTTCAAGCTCCTTGAAATTTTTGATTCCCTAGAGAAGCTGAAGCCTGGATTCTCGGAGGTTTTTGATGGCGAATCGGGGACTGACATTTGTACAAGATTCAGGGAGCTGGAGAAGCTACTTGTTCATGCTTCAAGTAAAGTTTTTTGGGAATTTGGGCTCCAAATTGAAGGCAATTCTGATGGAATGCCTCCACCACCAGATGGTTCGGTGCCGAAAATAGTAAGGTACGCAGTGAACTACTTGAAATACTTAGCAACTGAAAACTACAGCATCCCCATGGCGAAAGTTCTCAGAACGGAGCAAATCTGGAAAGCTGGGATCTTGTCGAAGCCAGAAACCTACGAGAACTTACTCAAAGACGCTATTTGCAACATCATGGAAGCTCTGCAGCGAAACGTGGAGTCGAAACGTTCCGGCTACAACAACAGCAGCAGGGAGAGGGACAAAATGGGCATTTCAATTCCCATACAACACGTGTTCCGCATGAACACGTACTGGTACATTTACATGCGGACGAGGAACACGGAGCTCGGGAAGCTGTTGGGCGACGGCTACATGAGGAACAACTACAAGGTTGTCGCCGAGGAATCCGCTTACTTGTACCAGAAGCAATCGTGGGCCCCGCTGGTGAGGATTCTGGAGAAGGACGATTTGGAGGAGAAGCAGAGCAAGGAGGAGAAGGCAGCGAAGGCGGCGCTGGTGAGGGTGAAAATCGAGGCGTTCATGAAGGGCTTGGACGACATTTCGAAGAGGCATAGAGGGTTTTGTGTAATTCCGGACGTTGATTTGCGGGAGCAGATTAGGGCGGCTACCGTGAAGCTGGTGATTCCTGCCTACACCGAGTTCGTGAACTCGTTCTCGGGGTTGTTGCAGGGGAAGAGTTATCTGAGTCCAGACTCGGTTCGGGAGTTTTTGGAGAAAGTTTTTTACGGTGGGGATGGGAAGCTTAAGCGGAGAGGGTCTAAGGATCGGACGGGTGGTGGGAGCTCAGCGATGGATGGTGTTGTGAAGGACCATCGACGGTCTAGATCAAGTACTAGTGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006887 GO:0008150 GO:0009987 GO:0016192 GO:0032940 GO:0046903 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Exo70

Protein Analysis

723

Amino Acids

82.27

Weight (kDa)

6.24

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo70_C PF03081 309 - 677 1.7e-80 Exo70 exocyst complex subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015557)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32950
malus_domestica MD08G1081000.v1.1 MD15G1068000.v1.1
prunus_persica Prupe.1G422000_v2.0.a1
pyrus_communis pycom15g06440
rosa_chinensis RchiOBHm_Chr6g0309751
rosa_laevigata RLG00000010538
rosa_multiflora Rmu_sc0007632.1_g000013
rosa_roxburghii Rroxscaffold_7G00159210
rosa_rugosa Rorug06G0374200
rosa_samantha Rh6AG487800 Rh6BG497400 Rh6CG502100 Rh6CG502200 Rh6DG488400
rosa_wichuraiana Rw6G042480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1327
AccB7I CCANNNNNTGG 1 cut(s) 1319
AccI GTMKAC 1 cut(s) 613
AccII CGCG 2 cut(s) 305, 581
AccIII TCCGGA 1 cut(s) 1894
AciI CCGC 9 cut(s) 305, 1600, 1630, 1710, 1742, 1811, 1909, 1925, 2071
AclI AACGTT 1 cut(s) 1528
AclWI GGATC 6 cut(s) 306, 384, 922, 935, 1445, 2094
AcoI YGGCCR 2 cut(s) 822, 1022
AcsI RAATTY 3 cut(s) 589, 1134, 1268
AcuI CTGAAG 2 cut(s) 1054, 1178
AcvI CACGTG 1 cut(s) 1594
AcyI GRCGYC 2 cut(s) 5, 549
AfiI CCNNNNNNNGG 5 cut(s) 30, 469, 502, 1092, 1319
AflII CTTAAG 1 cut(s) 2066
AflIII ACRYGT 3 cut(s) 1591, 1593, 1608
AhlI ACTAGT 1 cut(s) 2156
AjnI CCWGG 3 cut(s) 243, 282, 1162
AjuI GAANNNNNNNTTGG 2 cut(s) 1754, 1786
AloI GAACNNNNNNTCC 2 cut(s) 110, 142
Alw21I GWGCWC 3 cut(s) 945, 1650, 2108
Alw26I GTCTC 5 cut(s) 269, 302, 500, 530, 902
AlwI GGATC 6 cut(s) 306, 384, 922, 935, 1445, 2094
AlwNI CAGNNNCTG 2 cut(s) 436, 1117
Ama87I CYCGRG 3 cut(s) 44, 1649, 1978
Aor13HI TCCGGA 1 cut(s) 1894
AoxI GGCC 5 cut(s) 195, 240, 822, 1022, 1737
ApaI GGGCCC 1 cut(s) 1741
ApeKI GCWGC 7 cut(s) 436, 583, 656, 761, 1507, 1548, 1802
ApoI RAATTY 3 cut(s) 589, 1134, 1268
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
Asp700I GAANNNNTTC 5 cut(s) 105, 147, 745, 1118, 1973
AspA2I CCTAGG 1 cut(s) 994
AspLEI GCGC 4 cut(s) 250, 529, 583, 1816
AspS9I GGNCC 4 cut(s) 241, 1737, 1738, 2131
AsuC2I CCSGG 2 cut(s) 848, 1093
AsuHPI GGTGA 9 cut(s) 428, 485, 526, 817, 1091, 1759, 1831, 1837, 1954
AsuII TTCGAA 2 cut(s) 129, 1865
AsuNHI GCTAGC 1 cut(s) 49
AvaI CYCGRG 3 cut(s) 44, 1649, 1978
AvaII GGWCC 1 cut(s) 2131
AvrII CCTAGG 1 cut(s) 994
BaeGI GKGCMC 1 cut(s) 1741
BalI TGGCCA 2 cut(s) 824, 1024
BamHI GGATCC 1 cut(s) 927
BanI GGYRCC 1 cut(s) 1327
BanII GRGCYC 6 cut(s) 945, 1110, 1278, 1650, 1741, 2108
BbrPI CACGTG 1 cut(s) 1594
Bbv12I GWGCWC 3 cut(s) 945, 1650, 2108
BbvCI CCTCAGC 1 cut(s) 500
BbvI GCAGC 7 cut(s) 423, 570, 643, 748, 1519, 1560, 1814
BccI CCATC 9 cut(s) 109, 287, 1178, 1292, 1313, 2051, 2107, 2111, 2142
BceAI ACGGC 4 cut(s) 102, 356, 1070, 1684
BciT130I CCWGG 3 cut(s) 245, 284, 1164
BcnI CCSGG 2 cut(s) 848, 1093
BcoDI GTCTC 5 cut(s) 269, 302, 500, 530, 902
BcuI ACTAGT 1 cut(s) 2156
BfaI CTAG 7 cut(s) 50, 188, 758, 995, 1148, 2145, 2157
BfmI CTRYAG 2 cut(s) 1384, 1505
BfoI RGCGCY 3 cut(s) 251, 530, 1817
BfrI CTTAAG 1 cut(s) 2066
BisI GCNGC 9 cut(s) 437, 584, 657, 762, 1508, 1549, 1803, 1812, 1926
BlnI CCTAGG 1 cut(s) 994
BlpI GCTNAGC 1 cut(s) 2107
BlsI GCNGC 9 cut(s) 438, 585, 658, 763, 1509, 1550, 1804, 1813, 1927
BmcAI AGTACT 1 cut(s) 2155
Bme1390I CCNGG 5 cut(s) 245, 284, 848, 1093, 1164
Bme18I GGWCC 1 cut(s) 2131
BmeT110I CYCGRG 3 cut(s) 44, 1649, 1978
BmgT120I GGNCC 4 cut(s) 241, 1737, 1738, 2131
BmiI GGNNCC 5 cut(s) 929, 1277, 1329, 1739, 1740
BmrFI CCNGG 5 cut(s) 245, 284, 848, 1093, 1164
BmsI GCATC 2 cut(s) 795, 1398
BmtI GCTAGC 1 cut(s) 53
BpmI CTGGAG 3 cut(s) 638, 1247, 1778
Bpu10I CCTNAGC 1 cut(s) 500
Bpu1102I GCTNAGC 1 cut(s) 2107
Bpu14I TTCGAA 2 cut(s) 129, 1865
BpuEI CTTGAG 2 cut(s) 329, 506
BpuMI CCSGG 2 cut(s) 848, 1093
Bsa29I ATCGAT 1 cut(s) 789
BsaAI YACGTR 3 cut(s) 364, 1594, 1611
BsaBI GATNNNNATC 1 cut(s) 692
BsaHI GRCGYC 2 cut(s) 5, 549
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 6 cut(s) 192, 243, 893, 994, 1395, 1701
BsaWI WCCGGW 2 cut(s) 675, 1894
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 5 cut(s) 30, 469, 502, 1092, 1319
Bse118I RCCGGY 2 cut(s) 238, 495
Bse1I ACTGG 4 cut(s) 422, 481, 621, 1619
Bse8I GATNNNNATC 1 cut(s) 692
BseAI TCCGGA 1 cut(s) 1894
BseBI CCWGG 3 cut(s) 245, 284, 1164
BseCI ATCGAT 1 cut(s) 789
BseDI CCNNGG 6 cut(s) 192, 243, 893, 994, 1395, 1701
BseGI GGATG 4 cut(s) 279, 1389, 2062, 2122
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 5 cut(s) 30, 469, 502, 1092, 1319
BseMII CTCAG 6 cut(s) 491, 708, 751, 1423, 1997, 2121
BseNI ACTGG 4 cut(s) 422, 481, 621, 1619
BseRI GAGGAG 2 cut(s) 1790, 1808
BseSI GKGCMC 1 cut(s) 1741
BseXI GCAGC 7 cut(s) 423, 570, 643, 748, 1519, 1560, 1814
BseYI CCCAGC 1 cut(s) 1433
Bsh1236I CGCG 2 cut(s) 305, 581
Bsh1285I CGRYCG 2 cut(s) 477, 696
BshFI GGCC 5 cut(s) 197, 242, 824, 1024, 1739
BshNI GGYRCC 1 cut(s) 1327
BshVI ATCGAT 1 cut(s) 789
BsiEI CGRYCG 2 cut(s) 477, 696
BsiHKAI GWGCWC 3 cut(s) 945, 1650, 2108
BsiHKCI CYCGRG 3 cut(s) 44, 1649, 1978
BsiSI CCGG 8 cut(s) 239, 463, 496, 676, 848, 1093, 1533, 1895
BslFI GGGAC 4 cut(s) 727, 1026, 1210, 1574
BslI CCNNNNNNNGG 5 cut(s) 30, 469, 502, 1092, 1319
BsmAI GTCTC 5 cut(s) 269, 302, 500, 530, 902
BsmBI CGTCTC 3 cut(s) 302, 500, 530
BsmFI GGGAC 4 cut(s) 727, 1026, 1210, 1574
BsmI GAATGC 2 cut(s) 1034, 1308
BsnI GGCC 5 cut(s) 197, 242, 824, 1024, 1739
Bso31I GGTCTC 1 cut(s) 269
BsoBI CYCGRG 3 cut(s) 44, 1649, 1978
Bsp119I TTCGAA 2 cut(s) 129, 1865
Bsp120I GGGCCC 1 cut(s) 1737
Bsp1286I GDGCHC 6 cut(s) 945, 1110, 1278, 1650, 1741, 2108
Bsp13I TCCGGA 1 cut(s) 1894
Bsp1407I TGTACA 1 cut(s) 1208
Bsp143I GATC 8 cut(s) 40, 298, 376, 693, 927, 1437, 2086, 2147
Bsp1720I GCTNAGC 1 cut(s) 2107
Bsp19I CCATGG 3 cut(s) 192, 893, 1395
BspACI CCGC 9 cut(s) 305, 1600, 1630, 1710, 1742, 1811, 1909, 1925, 2071
BspANI GGCC 5 cut(s) 197, 242, 824, 1024, 1739
BspCNI CTCAG 6 cut(s) 492, 709, 750, 1422, 1998, 2120
BspDI ATCGAT 1 cut(s) 789
BspEI TCCGGA 1 cut(s) 1894
BspFNI CGCG 2 cut(s) 305, 581
BspHI TCATGA 1 cut(s) 1842
BspLI GGNNCC 5 cut(s) 929, 1277, 1329, 1739, 1740
BspMAI CTGCAG 1 cut(s) 1509
BspOI GCTAGC 1 cut(s) 53
BspPI GGATC 6 cut(s) 306, 384, 922, 935, 1445, 2094
BspT104I TTCGAA 2 cut(s) 129, 1865
BspT107I GGYRCC 1 cut(s) 1327
BspTI CTTAAG 1 cut(s) 2066
BspTNI GGTCTC 1 cut(s) 269
BsrFI RCCGGY 2 cut(s) 238, 495
BsrGI TGTACA 1 cut(s) 1208
BsrI ACTGG 4 cut(s) 422, 481, 621, 1619
BssAI RCCGGY 2 cut(s) 238, 495
BssECI CCNNGG 6 cut(s) 192, 243, 893, 994, 1395, 1701
BssMI GATC 8 cut(s) 40, 298, 376, 693, 927, 1437, 2086, 2147
BssNAI GTATAC 1 cut(s) 614
BssNI GRCGYC 2 cut(s) 5, 549
BssT1I CCWWGG 4 cut(s) 192, 893, 994, 1395
Bst1107I GTATAC 1 cut(s) 614
Bst2UI CCWGG 3 cut(s) 245, 284, 1164
Bst4CI ACNGT 3 cut(s) 1933, 2051, 2142
Bst6I CTCTTC 2 cut(s) 1862, 1991
BstACI GRCGYC 2 cut(s) 5, 549
BstAFI CTTAAG 1 cut(s) 2066
BstAUI TGTACA 1 cut(s) 1208
BstBAI YACGTR 3 cut(s) 364, 1594, 1611
BstBI TTCGAA 2 cut(s) 129, 1865
BstC8I GCNNGC 4 cut(s) 51, 240, 654, 945
BstDEI CTNAG 9 cut(s) 208, 500, 717, 737, 1369, 1409, 2006, 2082, 2107
BstDSI CCRYGG 3 cut(s) 192, 893, 1395
BstF5I GGATG 4 cut(s) 279, 1389, 2062, 2122
BstFNI CGCG 2 cut(s) 305, 581
BstH2I RGCGCY 3 cut(s) 251, 530, 1817
BstHHI GCGC 4 cut(s) 250, 529, 583, 1816
BstKTI GATC 8 cut(s) 43, 301, 379, 696, 930, 1440, 2089, 2150
BstMAI GTCTC 5 cut(s) 269, 302, 500, 530, 902
BstMBI GATC 8 cut(s) 40, 298, 376, 693, 927, 1437, 2086, 2147
BstMCI CGRYCG 2 cut(s) 477, 696
BstNI CCWGG 3 cut(s) 245, 284, 1164
BstNSI RCATGY 2 cut(s) 57, 1630
BstSCI CCNGG 5 cut(s) 243, 282, 846, 1091, 1162
BstSFI CTRYAG 2 cut(s) 1384, 1505
BstSLI GKGCMC 1 cut(s) 1741
BstUI CGCG 2 cut(s) 305, 581
BstV1I GCAGC 7 cut(s) 423, 570, 643, 748, 1519, 1560, 1814
BstX2I RGATCY 3 cut(s) 298, 927, 1437
BstYI RGATCY 3 cut(s) 298, 927, 1437
BstZ17I GTATAC 1 cut(s) 614
Bsu15I ATCGAT 1 cut(s) 789
BsuRI GGCC 5 cut(s) 197, 242, 824, 1024, 1739
BsuTUI ATCGAT 1 cut(s) 789
BtgI CCRYGG 3 cut(s) 192, 893, 1395
BtgZI GCGATG 1 cut(s) 2126
BtsCI GGATG 4 cut(s) 279, 1389, 2062, 2122
BtsI GCAGTG 2 cut(s) 1068, 1356
BtsIMutI CAGTG 4 cut(s) 429, 830, 1068, 1356
Cac8I GCNNGC 4 cut(s) 51, 240, 654, 945
CaiI CAGNNNCTG 2 cut(s) 436, 1117
CciI TCATGA 1 cut(s) 1842
CfoI GCGC 4 cut(s) 250, 529, 583, 1816
Cfr10I RCCGGY 2 cut(s) 238, 495
Cfr13I GGNCC 4 cut(s) 241, 1737, 1738, 2131
ClaI ATCGAT 1 cut(s) 789
CseI GACGC 5 cut(s) 147, 517, 557, 587, 1487
DdeI CTNAG 9 cut(s) 208, 500, 717, 737, 1369, 1409, 2006, 2082, 2107
DpnI GATC 8 cut(s) 42, 300, 378, 695, 929, 1439, 2088, 2149
DpnII GATC 8 cut(s) 40, 298, 376, 693, 927, 1437, 2086, 2147
EaeI YGGCCR 2 cut(s) 822, 1022
Eam1104I CTCTTC 2 cut(s) 1862, 1991
EarI CTCTTC 2 cut(s) 1862, 1991
Ecl136II GAGCTC 3 cut(s) 943, 1648, 2106
Eco130I CCWWGG 4 cut(s) 192, 893, 994, 1395
Eco24I GRGCYC 6 cut(s) 945, 1110, 1278, 1650, 1741, 2108
Eco31I GGTCTC 1 cut(s) 269
Eco47I GGWCC 1 cut(s) 2131
Eco53kI GAGCTC 3 cut(s) 943, 1648, 2106
Eco57I CTGAAG 2 cut(s) 1054, 1178
Eco72I CACGTG 1 cut(s) 1594
Eco88I CYCGRG 3 cut(s) 44, 1649, 1978
EcoICRI GAGCTC 3 cut(s) 943, 1648, 2106
EcoO109I RGGNCCY 1 cut(s) 1738
EcoRII CCWGG 3 cut(s) 243, 282, 1162
EcoT14I CCWWGG 4 cut(s) 192, 893, 994, 1395
EcoT22I ATGCAT 1 cut(s) 788
EcoT38I GRGCYC 6 cut(s) 945, 1110, 1278, 1650, 1741, 2108
ErhI CCWWGG 4 cut(s) 192, 893, 994, 1395
Esp3I CGTCTC 3 cut(s) 302, 500, 530
FalI AAGNNNNNCTT 4 cut(s) 555, 587, 945, 977
FaqI GGGAC 4 cut(s) 727, 1026, 1210, 1574
FauI CCCGC 2 cut(s) 1749, 1902
FauNDI CATATG 1 cut(s) 782
FblI GTMKAC 1 cut(s) 613
Fnu4HI GCNGC 9 cut(s) 437, 584, 657, 762, 1508, 1549, 1803, 1812, 1926
FokI GGATG 4 cut(s) 266, 1376, 2069, 2129
FriOI GRGCYC 6 cut(s) 945, 1110, 1278, 1650, 1741, 2108
Fsp4HI GCNGC 9 cut(s) 437, 584, 657, 762, 1508, 1549, 1803, 1812, 1926
FspBI CTAG 7 cut(s) 50, 188, 758, 995, 1148, 2145, 2157
GlaI GCGC 4 cut(s) 249, 528, 582, 1815
GluI GCNGC 9 cut(s) 437, 584, 657, 762, 1508, 1549, 1803, 1812, 1926
GsaI CCCAGC 1 cut(s) 1437
GsuI CTGGAG 3 cut(s) 638, 1247, 1778
HaeII RGCGCY 3 cut(s) 251, 530, 1817
HaeIII GGCC 5 cut(s) 197, 242, 824, 1024, 1739
HapII CCGG 8 cut(s) 239, 463, 496, 676, 848, 1093, 1533, 1895
HgaI GACGC 5 cut(s) 147, 517, 557, 587, 1487
HhaI GCGC 4 cut(s) 250, 529, 583, 1816
Hin1I GRCGYC 2 cut(s) 5, 549
Hin6I GCGC 4 cut(s) 248, 527, 581, 1814
HinP1I GCGC 4 cut(s) 248, 527, 581, 1814
HindIII AAGCTT 2 cut(s) 350, 2063
HpaII CCGG 8 cut(s) 239, 463, 496, 676, 848, 1093, 1533, 1895
HphI GGTGA 9 cut(s) 428, 485, 526, 817, 1091, 1759, 1831, 1837, 1954
Hpy166II GTNNAC 5 cut(s) 367, 547, 614, 1354, 1969
Hpy8I GTNNAC 5 cut(s) 367, 547, 614, 1354, 1969
Hpy99I CGWCG 3 cut(s) 88, 1670, 2142
HpyCH4III ACNGT 3 cut(s) 1933, 2051, 2142
HpyCH4IV ACGT 7 cut(s) 311, 363, 1515, 1528, 1593, 1610, 1899
HpyCH4V TGCA 7 cut(s) 439, 595, 652, 786, 1488, 1507, 1990
HpyF3I CTNAG 9 cut(s) 208, 500, 717, 737, 1369, 1409, 2006, 2082, 2107
HpySE526I ACGT 7 cut(s) 311, 363, 1515, 1528, 1593, 1610, 1899
Hsp92I GRCGYC 2 cut(s) 5, 549
HspAI GCGC 4 cut(s) 248, 527, 581, 1814
Kpn2I TCCGGA 1 cut(s) 1894
KroI GCCGGC 1 cut(s) 238
KroNI GCCGGC 1 cut(s) 240
Kzo9I GATC 8 cut(s) 40, 298, 376, 693, 927, 1437, 2086, 2147
LmnI GCTCC 9 cut(s) 178, 619, 1131, 1222, 1281, 1417, 1645, 1912, 2103
Lsp1109I GCAGC 7 cut(s) 423, 570, 643, 748, 1519, 1560, 1814
LweI GCATC 2 cut(s) 795, 1398
MaeI CTAG 7 cut(s) 50, 188, 758, 995, 1148, 2145, 2157
MaeII ACGT 7 cut(s) 311, 363, 1515, 1528, 1593, 1610, 1899
MaeIII GTNAC 2 cut(s) 232, 514
MalI GATC 8 cut(s) 42, 300, 378, 695, 929, 1439, 2088, 2149
MboI GATC 8 cut(s) 40, 298, 376, 693, 927, 1437, 2086, 2147
MboII GAAGA 8 cut(s) 98, 346, 422, 887, 985, 1060, 1879, 2008
MflI RGATCY 3 cut(s) 298, 927, 1437
MhlI GDGCHC 6 cut(s) 945, 1110, 1278, 1650, 1741, 2108
MlsI TGGCCA 2 cut(s) 824, 1024
MluNI TGGCCA 2 cut(s) 824, 1024
MlyI GAGTC 5 cut(s) 484, 511, 1529, 2009, 2017
MmeI TCCRAC 1 cut(s) 702
Mox20I TGGCCA 2 cut(s) 824, 1024
Mph1103I ATGCAT 1 cut(s) 788
MroI TCCGGA 1 cut(s) 1894
MroNI GCCGGC 1 cut(s) 238
MroXI GAANNNNTTC 5 cut(s) 105, 147, 745, 1118, 1973
MscI TGGCCA 2 cut(s) 824, 1024
MseI TTAA 3 cut(s) 627, 801, 2067
MslI CAYNNNNRTG 4 cut(s) 636, 1061, 1394, 1625
Msp20I TGGCCA 2 cut(s) 824, 1024
MspA1I CMGCKG 1 cut(s) 1744
MspCI CTTAAG 1 cut(s) 2066
MspI CCGG 8 cut(s) 239, 463, 496, 676, 848, 1093, 1533, 1895
MspR9I CCNGG 5 cut(s) 245, 284, 848, 1093, 1164
Mva1269I GAATGC 2 cut(s) 1034, 1308
MvaI CCWGG 3 cut(s) 245, 284, 1164
MvnI CGCG 2 cut(s) 305, 581
NaeI GCCGGC 1 cut(s) 240
NciI CCSGG 2 cut(s) 848, 1093
NcoI CCATGG 3 cut(s) 192, 893, 1395
NdeI CATATG 1 cut(s) 782
NdeII GATC 8 cut(s) 40, 298, 376, 693, 927, 1437, 2086, 2147
NgoMIV GCCGGC 1 cut(s) 238
NheI GCTAGC 1 cut(s) 49
NlaIV GGNNCC 5 cut(s) 929, 1277, 1329, 1739, 1740
NmeAIII GCCGAG 1 cut(s) 1726
NmuCI GTSAC 2 cut(s) 232, 514
NsiI ATGCAT 1 cut(s) 788
NspI RCATGY 2 cut(s) 57, 1630
NspV TTCGAA 2 cut(s) 129, 1865
PaeR7I CTCGAG 1 cut(s) 44
PagI TCATGA 1 cut(s) 1842
PcsI WCGNNNNNNNCGW 2 cut(s) 369, 1083
PctI GAATGC 2 cut(s) 1034, 1308
PdiI GCCGGC 1 cut(s) 240
PdmI GAANNNNTTC 5 cut(s) 105, 147, 745, 1118, 1973
PflFI GACNNNGTC 1 cut(s) 313
PflMI CCANNNNNTGG 1 cut(s) 1319
PfoI TCCNGGA 1 cut(s) 282
PkrI GCNGC 9 cut(s) 438, 585, 658, 763, 1509, 1550, 1804, 1813, 1927
Ple19I CGATCG 1 cut(s) 696
PleI GAGTC 5 cut(s) 484, 511, 1528, 2009, 2016
PmaCI CACGTG 1 cut(s) 1594
PmlI CACGTG 1 cut(s) 1594
PpsI GAGTC 5 cut(s) 484, 511, 1528, 2009, 2016
Ppu21I YACGTR 3 cut(s) 364, 1594, 1611
Psp124BI GAGCTC 3 cut(s) 945, 1650, 2108
Psp1406I AACGTT 1 cut(s) 1528
Psp6I CCWGG 3 cut(s) 243, 282, 1162
PspCI CACGTG 1 cut(s) 1594
PspFI CCCAGC 1 cut(s) 1433
PspGI CCWGG 3 cut(s) 243, 282, 1162
PspN4I GGNNCC 5 cut(s) 929, 1277, 1329, 1739, 1740
PspOMI GGGCCC 1 cut(s) 1737
PspPI GGNCC 4 cut(s) 241, 1737, 1738, 2131
PstI CTGCAG 1 cut(s) 1509
PstNI CAGNNNCTG 2 cut(s) 436, 1117
PsuI RGATCY 3 cut(s) 298, 927, 1437
PsyI GACNNNGTC 1 cut(s) 313
PvuI CGATCG 1 cut(s) 696
RseI CAYNNNNRTG 4 cut(s) 636, 1061, 1394, 1625
SacI GAGCTC 3 cut(s) 945, 1650, 2108
SaqAI TTAA 3 cut(s) 627, 801, 2067
SatI GCNGC 9 cut(s) 437, 584, 657, 762, 1508, 1549, 1803, 1812, 1926
Sau3AI GATC 8 cut(s) 40, 298, 376, 693, 927, 1437, 2086, 2147
Sau96I GGNCC 4 cut(s) 241, 1737, 1738, 2131
ScaI AGTACT 1 cut(s) 2155
SchI GAGTC 5 cut(s) 484, 511, 1529, 2009, 2017
ScrFI CCNGG 5 cut(s) 245, 284, 848, 1093, 1164
SduI GDGCHC 6 cut(s) 945, 1110, 1278, 1650, 1741, 2108
SfaNI GCATC 2 cut(s) 795, 1398
SfcI CTRYAG 2 cut(s) 1384, 1505
Sfr274I CTCGAG 1 cut(s) 44
SfuI TTCGAA 2 cut(s) 129, 1865
SinI GGWCC 1 cut(s) 2131
SlaI CTCGAG 1 cut(s) 44
SmiMI CAYNNNNRTG 4 cut(s) 636, 1061, 1394, 1625
SmlI CTYRAG 4 cut(s) 44, 344, 521, 2066
SmoI CTYRAG 4 cut(s) 44, 344, 521, 2066
SpeI ACTAGT 1 cut(s) 2156
SsiI CCGC 9 cut(s) 305, 1600, 1630, 1710, 1742, 1811, 1909, 1925, 2071
SspMI CTAG 7 cut(s) 50, 188, 758, 995, 1148, 2145, 2157
SstI GAGCTC 3 cut(s) 945, 1650, 2108
StyD4I CCNGG 5 cut(s) 243, 282, 846, 1091, 1162
StyI CCWWGG 4 cut(s) 192, 893, 994, 1395
TaaI ACNGT 3 cut(s) 1933, 2051, 2142
TaiI ACGT 7 cut(s) 314, 366, 1518, 1531, 1596, 1613, 1902
TaqII GACCGA 1 cut(s) 491
TatI WGTACW 3 cut(s) 483, 1208, 2153
TauI GCSGC 2 cut(s) 1814, 1928
Tru1I TTAA 3 cut(s) 627, 801, 2067
Tru9I TTAA 3 cut(s) 627, 801, 2067
TscAI CASTG 4 cut(s) 429, 837, 1068, 1356
TseFI GTSAC 2 cut(s) 232, 514
TseI GCWGC 7 cut(s) 436, 583, 656, 761, 1507, 1548, 1802
Tsp45I GTSAC 2 cut(s) 232, 514
TspDTI ATGAA 5 cut(s) 900, 1232, 1619, 1831, 1859
TspGWI ACGGA 3 cut(s) 554, 1430, 1658
TspRI CASTG 4 cut(s) 429, 837, 1068, 1356
Tth111I GACNNNGTC 1 cut(s) 313
Van91I CCANNNNNTGG 1 cut(s) 1319
Vha464I CTTAAG 1 cut(s) 2066
VpaK11BI GGWCC 1 cut(s) 2131
XapI RAATTY 3 cut(s) 589, 1134, 1268
XbaI TCTAGA 1 cut(s) 2144
XceI RCATGY 2 cut(s) 57, 1630
XhoI CTCGAG 1 cut(s) 44
XmaJI CCTAGG 1 cut(s) 994
XmiI GTMKAC 1 cut(s) 613
XmnI GAANNNNTTC 5 cut(s) 105, 147, 745, 1118, 1973
XspI CTAG 7 cut(s) 50, 188, 758, 995, 1148, 2145, 2157
ZrmI AGTACT 1 cut(s) 2155
Zsp2I ATGCAT 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.