Rw6G042480

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015557
Physical Location & Seq
Reverse (-)
65915274 .. 65917758
2485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G042480.1

Sequence Viewer

Length: 2109 bp
ATGGCGACCAATGAAGAAGATTCAAGGCTCTCTGACATTCAGCTAGCCTGCACATACTTGAGAACCCTACTCAAAGCCTCTGCCTTGATGGAACAGAATCTGAAAAAGATGGACTACAAGTTTAACCTCATTGACGAAACACTAACGTCAGCCTCAAGAAGAGTAGCTCCATTACAGTCCATGTCCATGGCCTCCAGGGCCCTGGAAACCAGAATCAACCGATCTGTCACGCCGGCCCTTGCGCTCCTCGACAACTTCAAGCGATCAGAGTCTATACAGGACAAGCTTCTCGACTTGTCATCGAAGTTGCCAAACGAGAAGAACATGAACAAGAGGCTCAAGACGCTCATCAAGTACGTGGACTGTGTGGATCAGCTCAAGGCGGCGATAAGCTCCATATGCGAGGAGGGCGAGCCGGTGATCCAGAGGCTTCAGGAGGTGGTGGAGTTTCTCAGCCGGACGAAGGCGACTGATCAGTACAGGATCCATCGGTTGAGGGAGACTCTGATCACTTTGAAGGCCTTGTATGAGACAGAGGTGGATGCTATGAGATTTGAAGGGCTGCTTGATGAGGCTTTGCTGAATTTGCAGGATGAGTATGAGAATATATTGCAGCAAATAAAGCATCAAAATATAGTGGATCAATCACAGGCTGTAGATAAGGATAATGAAGGTGATGATCAGATGGTGATCACGGGTTCTAATTTGGGCACTGAGTTGGAAATTGAACTTCTAAGGCGGATCTCACAAACCCTAGCTGCCGAAGATTGTTTGGATATATGCATCGATATTTATGTTAAGGTGAGATACAGAAGAGTGGCAAAAGCACTAATGCGGCTAAACCCAGAGTACTTGAGAACACACAGTCCGGAAGAAATAGACGAAATGGCTTGGGAGAATTTAGAGACGGCCATAAGCCTTTGGATCCAACACTTCGACCTCGCAGTGAAAGCAGTGCTGGTCTCAGAGAAGAATTTCTGCGAAAAAGTACTGGGAGGCATAATGGACGGGCTGGTCTGGCCAGAATGCTTTGTAAAAATCGCAGACAAAATCATGGCGGTGTTCTTCAGATTCGGAGAAGGCGTAGCCCGGAGCAGCAAAGAGCCACAAAAACTGTTCAAGTTGCTAGACATGTTCGAGTCCTTGGAGAAACTCAAGCCTGCATTCTCTCAAGTCTTCGAAGGTGAAGCAGGGACTGACATTTGCGTTCGATTCAGAGAACTCGAGAAGCTTCTGATTCACGCTTCCAGTAAAGTTTTCTGGGAGTTTGGGCTTCAAATTGAAGGAAACTCAGATGGGGTACCTCCCCCGCAAGATGGGTCAGTCCCCAAAATTGTAAGGTACGCTGTGAATTACATGAAGTACTTAGCAACAGAGGCTTACTCTGTTCCCATGGCCAGAGTCCTCAGAACAGAGCAGATTTGGAAAGCTGGGATTCTGTCCAGACCCCAATCGGACGAGAACCTTCTCAGAGATGCCGTTTCCCACATCATGGAAGCTCTGCAGAGGAATGTAGAATCGAAACGGTCGGGTTATCGGGTCGATAAGTGGGCTCTGCCCCATGTTTTTGCAATGAACACGTACTGGTATATTTACATGAGGACCAAAAACACAGAGCTAGGGAAGCTCGTGGGAGACCAATACATGAAGAAAAATTACAAAGTAATAGCAGAGGAATCGGCTTACATGTATCAGAAGATAGTGTGGGTTCCTCTGGTTAGAGTTTTGGAGAAAGATGATGATTTGGAGCTGGAGAGCAAGGAAGCAATGGTTAGGGCGAAAATGGAGGCGTTTATGAAGGGCTTGGATGATGTATCAAAGAGGCACAAAGAGTACTATAGTATTCCTGACGAAGATTTGAGGGAGCAGATTAGATTGGCGACGGTGAAGCTTGTGGTTCCAGCGTACAGTAAGTTCTTGGACTCGTTCTCGGTTTCGTTACCGGGGAAGTCGTCGTATTTGAGCCCTGAGTCAGTTCAGCAGTTGCTGTGTCAAATATTTGAGGGTGGGGAGGTGAAGCTTAAGAGGCGTGGGTCTAGGGATCGGACGGCGGTGGACGGTGCTGTTAAGGGCTTTCGACGCACTAGGTCAAATACCAGTGAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006887 GO:0008150 GO:0009987 GO:0016192 GO:0032940 GO:0046903 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Exo70

Protein Analysis

702

Amino Acids

80.68

Weight (kDa)

6.11

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo70_C PF03081 308 - 663 8.1e-80 Exo70 exocyst complex subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015557)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32950
malus_domestica MD08G1081000.v1.1 MD15G1068000.v1.1
prunus_persica Prupe.1G422000_v2.0.a1
pyrus_communis pycom15g06440
rosa_chinensis RchiOBHm_Chr6g0309751
rosa_laevigata RLG00000010538
rosa_multiflora Rmu_sc0007632.1_g000013
rosa_roxburghii Rroxscaffold_7G00159210
rosa_rugosa Rorug06G0374200
rosa_samantha Rh6AG487800 Rh6BG497400 Rh6CG502100 Rh6CG502200 Rh6DG488400
rosa_wichuraiana Rw6G042480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1013
Acc65I GGTACC 1 cut(s) 1300
AccB1I GGYRCC 1 cut(s) 1300
AccB7I CCANNNNNTGG 1 cut(s) 1492
AccIII TCCGGA 1 cut(s) 868
AciI CCGC 6 cut(s) 383, 739, 835, 1058, 1310, 2051
AclWI GGATC 9 cut(s) 378, 415, 478, 491, 648, 749, 919, 932, 2049
AcoI YGGCCR 3 cut(s) 909, 1019, 1395
AcsI RAATTY 3 cut(s) 583, 898, 973
AcuI CTGAAG 2 cut(s) 416, 1051
AfiI CCNNNNNNNGG 3 cut(s) 463, 1316, 1492
AflII CTTAAG 1 cut(s) 2021
AflIII ACRYGT 3 cut(s) 1131, 1578, 1686
AgsI TTSAA 8 cut(s) 24, 259, 517, 557, 728, 1120, 1277, 1283
AjnI CCWGG 2 cut(s) 194, 201
AloI GAACNNNNNNTCC 2 cut(s) 850, 882
Alw26I GTCTC 5 cut(s) 494, 524, 899, 967, 1629
AlwI GGATC 9 cut(s) 378, 415, 478, 491, 648, 749, 919, 932, 2049
AlwNI CAGNNNCTG 3 cut(s) 100, 1196, 1987
Ama87I CYCGRG 1 cut(s) 1223
Aor13HI TCCGGA 1 cut(s) 868
AoxI GGCC 7 cut(s) 189, 198, 234, 519, 909, 1019, 1395
ApaI GGGCCC 1 cut(s) 202
ApeKI GCWGC 4 cut(s) 562, 613, 758, 1095
ApoI RAATTY 3 cut(s) 583, 898, 973
Asp700I GAANNNNTTC 1 cut(s) 974
Asp718I GGTACC 1 cut(s) 1300
AspLEI GCGC 1 cut(s) 244
AspS9I GGNCC 4 cut(s) 198, 199, 235, 1602
AsuC2I CCSGG 2 cut(s) 1090, 1944
AsuHPI GGTGA 7 cut(s) 430, 686, 700, 814, 1196, 1897, 2026
AsuII TTCGAA 1 cut(s) 1179
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 1223
AvaII GGWCC 1 cut(s) 1602
BaeGI GKGCMC 2 cut(s) 202, 713
BalI TGGCCA 2 cut(s) 1021, 1397
BamHI GGATCC 2 cut(s) 483, 924
BanI GGYRCC 1 cut(s) 1300
BanII GRGCYC 3 cut(s) 202, 1555, 1967
BarI GAAGNNNNNNTAC 2 cut(s) 1640, 1672
BauI CACGAG 1 cut(s) 1628
BbsI GAAGAC 1 cut(s) 1168
BbvI GCAGC 4 cut(s) 549, 625, 745, 1107
BccI CCATC 6 cut(s) 82, 103, 495, 679, 1289, 1310
BceAI ACGGC 3 cut(s) 924, 1463, 2064
BcgI CGANNNNNNTGC 2 cut(s) 1659, 1693
BciT130I CCWGG 2 cut(s) 196, 203
BclI TGATCA 4 cut(s) 472, 507, 679, 690
BcnI CCSGG 2 cut(s) 1090, 1944
BcoDI GTCTC 5 cut(s) 494, 524, 899, 967, 1629
BfaI CTAG 6 cut(s) 44, 755, 1127, 1619, 2037, 2085
BfmI CTRYAG 3 cut(s) 654, 1502, 1837
BfrI CTTAAG 1 cut(s) 2021
BglI GCCNNNNNGGC 1 cut(s) 197
BisI GCNGC 6 cut(s) 384, 563, 614, 759, 836, 1096
BlsI GCNGC 6 cut(s) 385, 564, 615, 760, 837, 1097
BmcAI AGTACT 4 cut(s) 851, 990, 1364, 1835
Bme1390I CCNGG 4 cut(s) 196, 203, 1090, 1944
Bme18I GGWCC 1 cut(s) 1602
BmeT110I CYCGRG 1 cut(s) 1223
BmgT120I GGNCC 4 cut(s) 198, 199, 235, 1602
BmiI GGNNCC 6 cut(s) 200, 485, 926, 1302, 1710, 1899
BmrFI CCNGG 4 cut(s) 196, 203, 1090, 1944
BmrI ACTGGG 1 cut(s) 1001
BmsI GCATC 4 cut(s) 532, 634, 792, 1465
BmtI GCTAGC 1 cut(s) 47
BmuI ACTGGG 1 cut(s) 1001
BpiI GAAGAC 1 cut(s) 1168
BplI GAGNNNNNCTC 4 cut(s) 487, 519, 1367, 1399
BpmI CTGGAG 2 cut(s) 178, 1772
Bpu14I TTCGAA 1 cut(s) 1179
BpuEI CTTGAG 7 cut(s) 79, 139, 323, 362, 874, 1139, 1155
BpuMI CCSGG 2 cut(s) 1090, 1944
Bsa29I ATCGAT 1 cut(s) 786
BsaAI YACGTR 2 cut(s) 358, 1581
BsaBI GATNNNNATC 1 cut(s) 689
BsaI GGTCTC 2 cut(s) 967, 1629
BsaJI CCNNGG 6 cut(s) 186, 195, 201, 1143, 1392, 1943
BsaWI WCCGGW 1 cut(s) 868
BsaXI ACNNNNNCTCC 2 cut(s) 1068, 1098
Bsc4I CCNNNNNNNGG 3 cut(s) 463, 1316, 1492
Bse118I RCCGGY 2 cut(s) 232, 415
Bse1I ACTGG 4 cut(s) 996, 1248, 1589, 2097
Bse3DI GCAATG 2 cut(s) 1578, 1773
Bse8I GATNNNNATC 1 cut(s) 689
BseAI TCCGGA 1 cut(s) 868
BseBI CCWGG 2 cut(s) 196, 203
BseCI ATCGAT 1 cut(s) 786
BseDI CCNNGG 6 cut(s) 186, 195, 201, 1143, 1392, 1943
BseGI GGATG 3 cut(s) 547, 598, 1813
BseJI GATNNNNATC 1 cut(s) 689
BseLI CCNNNNNNNGG 3 cut(s) 463, 1316, 1492
BseMI GCAATG 2 cut(s) 1578, 1773
BseMII CTCAG 7 cut(s) 466, 705, 978, 1305, 1420, 1483, 1959
BseNI ACTGG 4 cut(s) 996, 1248, 1589, 2097
BseRI GAGGAG 2 cut(s) 236, 419
BseSI GKGCMC 2 cut(s) 202, 713
BseXI GCAGC 4 cut(s) 549, 625, 745, 1107
BseYI CCCAGC 1 cut(s) 1430
BsgI GTGCAG 1 cut(s) 34
Bsh1285I CGRYCG 1 cut(s) 1529
BshFI GGCC 7 cut(s) 191, 200, 236, 521, 911, 1021, 1397
BshNI GGYRCC 1 cut(s) 1300
BshVI ATCGAT 1 cut(s) 786
BsiEI CGRYCG 1 cut(s) 1529
BsiHKCI CYCGRG 1 cut(s) 1223
BsiSI CCGG 6 cut(s) 233, 416, 457, 869, 1090, 1943
BslFI GGGAC 2 cut(s) 1207, 1310
BslI CCNNNNNNNGG 3 cut(s) 463, 1316, 1492
BsmAI GTCTC 5 cut(s) 494, 524, 899, 967, 1629
BsmBI CGTCTC 1 cut(s) 899
BsmFI GGGAC 2 cut(s) 1207, 1310
BsmI GAATGC 2 cut(s) 1031, 1163
BsnI GGCC 7 cut(s) 191, 200, 236, 521, 911, 1021, 1397
Bso31I GGTCTC 2 cut(s) 967, 1629
BsoBI CYCGRG 1 cut(s) 1223
Bsp119I TTCGAA 1 cut(s) 1179
Bsp120I GGGCCC 1 cut(s) 198
Bsp1286I GDGCHC 4 cut(s) 202, 713, 1555, 1967
Bsp13I TCCGGA 1 cut(s) 868
Bsp19I CCATGG 2 cut(s) 186, 1392
BspACI CCGC 6 cut(s) 383, 739, 835, 1058, 1310, 2051
BspANI GGCC 7 cut(s) 191, 200, 236, 521, 911, 1021, 1397
BspCNI CTCAG 7 cut(s) 465, 706, 977, 1304, 1419, 1482, 1960
BspDI ATCGAT 1 cut(s) 786
BspEI TCCGGA 1 cut(s) 868
BspLI GGNNCC 6 cut(s) 200, 485, 926, 1302, 1710, 1899
BspMAI CTGCAG 1 cut(s) 1506
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 9 cut(s) 378, 415, 478, 491, 648, 749, 919, 932, 2049
BspT104I TTCGAA 1 cut(s) 1179
BspT107I GGYRCC 1 cut(s) 1300
BspTI CTTAAG 1 cut(s) 2021
BspTNI GGTCTC 2 cut(s) 967, 1629
BsrDI GCAATG 2 cut(s) 1578, 1773
BsrFI RCCGGY 2 cut(s) 232, 415
BsrI ACTGG 4 cut(s) 996, 1248, 1589, 2097
BssAI RCCGGY 2 cut(s) 232, 415
BssECI CCNNGG 6 cut(s) 186, 195, 201, 1143, 1392, 1943
BssSI CACGAG 1 cut(s) 1628
BssT1I CCWWGG 3 cut(s) 186, 1143, 1392
Bst2BI CACGAG 1 cut(s) 1628
Bst2UI CCWGG 2 cut(s) 196, 203
Bst4CI ACNGT 8 cut(s) 177, 365, 866, 1116, 1527, 1885, 1910, 2060
Bst6I CTCTTC 2 cut(s) 154, 808
BstAFI CTTAAG 1 cut(s) 2021
BstBAI YACGTR 2 cut(s) 358, 1581
BstBI TTCGAA 1 cut(s) 1179
BstC8I GCNNGC 5 cut(s) 45, 49, 234, 413, 1161
BstDEI CTNAG 9 cut(s) 452, 714, 734, 964, 1291, 1366, 1406, 1469, 1968
BstDSI CCRYGG 2 cut(s) 186, 1392
BstF5I GGATG 3 cut(s) 547, 598, 1813
BstHHI GCGC 1 cut(s) 244
BstMAI GTCTC 5 cut(s) 494, 524, 899, 967, 1629
BstMCI CGRYCG 1 cut(s) 1529
BstNI CCWGG 2 cut(s) 196, 203
BstNSI RCATGY 2 cut(s) 1135, 1690
BstSCI CCNGG 4 cut(s) 194, 201, 1088, 1942
BstSFI CTRYAG 3 cut(s) 654, 1502, 1837
BstSLI GKGCMC 2 cut(s) 202, 713
BstV1I GCAGC 4 cut(s) 549, 625, 745, 1107
BstV2I GAAGAC 1 cut(s) 1168
BstX2I RGATCY 3 cut(s) 483, 741, 924
BstXI CCANNNNNNTGG 2 cut(s) 187, 202
BstYI RGATCY 3 cut(s) 483, 741, 924
Bsu15I ATCGAT 1 cut(s) 786
BsuRI GGCC 7 cut(s) 191, 200, 236, 521, 911, 1021, 1397
BsuTUI ATCGAT 1 cut(s) 786
BtgI CCRYGG 2 cut(s) 186, 1392
BtsCI GGATG 3 cut(s) 547, 598, 1813
BtsI GCAGTG 2 cut(s) 951, 960
BtsIMutI CAGTG 4 cut(s) 711, 951, 960, 2104
Cac8I GCNNGC 5 cut(s) 45, 49, 234, 413, 1161
CaiI CAGNNNCTG 3 cut(s) 100, 1196, 1987
CfoI GCGC 1 cut(s) 244
Cfr10I RCCGGY 2 cut(s) 232, 415
Cfr13I GGNCC 4 cut(s) 198, 199, 235, 1602
ClaI ATCGAT 1 cut(s) 786
CseI GACGC 2 cut(s) 352, 2088
DdeI CTNAG 9 cut(s) 452, 714, 734, 964, 1291, 1366, 1406, 1469, 1968
DrdI GACNNNNNNGTC 1 cut(s) 1013
DseDI GACNNNNNNGTC 1 cut(s) 1013
EaeI YGGCCR 3 cut(s) 909, 1019, 1395
Eam1104I CTCTTC 2 cut(s) 154, 808
EarI CTCTTC 2 cut(s) 154, 808
EciI GGCGGA 1 cut(s) 754
Eco130I CCWWGG 3 cut(s) 186, 1143, 1392
Eco147I AGGCCT 1 cut(s) 521
Eco24I GRGCYC 3 cut(s) 202, 1555, 1967
Eco31I GGTCTC 2 cut(s) 967, 1629
Eco47I GGWCC 1 cut(s) 1602
Eco57I CTGAAG 2 cut(s) 416, 1051
Eco88I CYCGRG 1 cut(s) 1223
EcoO109I RGGNCCY 2 cut(s) 198, 199
EcoRII CCWGG 2 cut(s) 194, 201
EcoT14I CCWWGG 3 cut(s) 186, 1143, 1392
EcoT22I ATGCAT 1 cut(s) 785
EcoT38I GRGCYC 3 cut(s) 202, 1555, 1967
ErhI CCWWGG 3 cut(s) 186, 1143, 1392
Esp3I CGTCTC 1 cut(s) 899
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FaqI GGGAC 2 cut(s) 1207, 1310
FauI CCCGC 1 cut(s) 1317
FauNDI CATATG 1 cut(s) 398
FbaI TGATCA 4 cut(s) 472, 507, 679, 690
Fnu4HI GCNGC 6 cut(s) 384, 563, 614, 759, 836, 1096
FokI GGATG 3 cut(s) 554, 605, 1820
FriOI GRGCYC 3 cut(s) 202, 1555, 1967
Fsp4HI GCNGC 6 cut(s) 384, 563, 614, 759, 836, 1096
FspBI CTAG 6 cut(s) 44, 755, 1127, 1619, 2037, 2085
GlaI GCGC 1 cut(s) 243
GluI GCNGC 6 cut(s) 384, 563, 614, 759, 836, 1096
GsaI CCCAGC 1 cut(s) 1434
GsuI CTGGAG 2 cut(s) 178, 1772
HaeIII GGCC 7 cut(s) 191, 200, 236, 521, 911, 1021, 1397
HapII CCGG 6 cut(s) 233, 416, 457, 869, 1090, 1943
HgaI GACGC 2 cut(s) 352, 2088
HhaI GCGC 1 cut(s) 244
Hin6I GCGC 1 cut(s) 242
HinP1I GCGC 1 cut(s) 242
HindIII AAGCTT 4 cut(s) 284, 1229, 1889, 2018
HpaII CCGG 6 cut(s) 233, 416, 457, 869, 1090, 1943
HphI GGTGA 7 cut(s) 430, 686, 700, 814, 1196, 1897, 2026
Hpy166II GTNNAC 2 cut(s) 361, 2056
Hpy188III TCNNGA 9 cut(s) 156, 290, 340, 424, 434, 869, 1225, 1443, 1847
Hpy8I GTNNAC 2 cut(s) 361, 2056
Hpy99I CGWCG 3 cut(s) 1885, 1957, 2082
HpyAV CCTTC 9 cut(s) 457, 511, 551, 665, 1073, 1175, 1277, 1475, 1792
HpyCH4III ACNGT 8 cut(s) 177, 365, 866, 1116, 1527, 1885, 1910, 2060
HpyCH4IV ACGT 3 cut(s) 146, 357, 1580
HpyCH4V TGCA 7 cut(s) 51, 589, 613, 783, 1163, 1504, 1571
HpyF3I CTNAG 9 cut(s) 452, 714, 734, 964, 1291, 1366, 1406, 1469, 1968
HpySE526I ACGT 3 cut(s) 146, 357, 1580
HspAI GCGC 1 cut(s) 242
Kpn2I TCCGGA 1 cut(s) 868
KpnI GGTACC 1 cut(s) 1304
KroI GCCGGC 1 cut(s) 232
KroNI GCCGGC 1 cut(s) 234
Ksp22I TGATCA 4 cut(s) 472, 507, 679, 690
LmnI GCTCC 6 cut(s) 172, 249, 398, 1092, 1747, 1864
Lsp1109I GCAGC 4 cut(s) 549, 625, 745, 1107
LweI GCATC 4 cut(s) 532, 634, 792, 1465
MaeI CTAG 6 cut(s) 44, 755, 1127, 1619, 2037, 2085
MaeII ACGT 3 cut(s) 146, 357, 1580
MaeIII GTNAC 2 cut(s) 226, 1938
MflI RGATCY 3 cut(s) 483, 741, 924
MhlI GDGCHC 4 cut(s) 202, 713, 1555, 1967
MlsI TGGCCA 2 cut(s) 1021, 1397
MluCI AATT 9 cut(s) 583, 703, 723, 898, 973, 1278, 1332, 1351, 1654
MluNI TGGCCA 2 cut(s) 1021, 1397
MlyI GAGTC 6 cut(s) 278, 496, 1148, 1410, 1916, 1979
MmeI TCCRAC 2 cut(s) 699, 952
Mox20I TGGCCA 2 cut(s) 1021, 1397
Mph1103I ATGCAT 1 cut(s) 785
MroI TCCGGA 1 cut(s) 868
MroNI GCCGGC 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 974
MscI TGGCCA 2 cut(s) 1021, 1397
MseI TTAA 4 cut(s) 123, 798, 2022, 2067
MslI CAYNNNNRTG 2 cut(s) 185, 1058
Msp20I TGGCCA 2 cut(s) 1021, 1397
MspCI CTTAAG 1 cut(s) 2021
MspI CCGG 6 cut(s) 233, 416, 457, 869, 1090, 1943
MspR9I CCNGG 4 cut(s) 196, 203, 1090, 1944
Mva1269I GAATGC 2 cut(s) 1031, 1163
MvaI CCWGG 2 cut(s) 196, 203
NaeI GCCGGC 1 cut(s) 234
NciI CCSGG 2 cut(s) 1090, 1944
NcoI CCATGG 2 cut(s) 186, 1392
NdeI CATATG 1 cut(s) 398
NgoMIV GCCGGC 1 cut(s) 232
NheI GCTAGC 1 cut(s) 43
NlaIV GGNNCC 6 cut(s) 200, 485, 926, 1302, 1710, 1899
NmuCI GTSAC 1 cut(s) 226
NsiI ATGCAT 1 cut(s) 785
NspI RCATGY 2 cut(s) 1135, 1690
NspV TTCGAA 1 cut(s) 1179
PaeR7I CTCGAG 1 cut(s) 1223
PceI AGGCCT 1 cut(s) 521
PciI ACATGT 2 cut(s) 1131, 1686
PcsI WCGNNNNNNNCGW 1 cut(s) 1080
PctI GAATGC 2 cut(s) 1031, 1163
PdiI GCCGGC 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 974
PfeI GAWTC 9 cut(s) 20, 97, 213, 1071, 1212, 1237, 1435, 1517, 1676
PflMI CCANNNNNTGG 1 cut(s) 1492
PkrI GCNGC 6 cut(s) 385, 564, 615, 760, 837, 1097
PleI GAGTC 6 cut(s) 277, 496, 1147, 1409, 1916, 1978
PpsI GAGTC 6 cut(s) 277, 496, 1147, 1409, 1916, 1978
Ppu21I YACGTR 2 cut(s) 358, 1581
PscI ACATGT 2 cut(s) 1131, 1686
Psp6I CCWGG 2 cut(s) 194, 201
PspFI CCCAGC 1 cut(s) 1430
PspGI CCWGG 2 cut(s) 194, 201
PspN4I GGNNCC 6 cut(s) 200, 485, 926, 1302, 1710, 1899
PspOMI GGGCCC 1 cut(s) 198
PspPI GGNCC 4 cut(s) 198, 199, 235, 1602
PsrI GAACNNNNNNTAC 2 cut(s) 1898, 1930
PstI CTGCAG 1 cut(s) 1506
PstNI CAGNNNCTG 3 cut(s) 100, 1196, 1987
PsuI RGATCY 3 cut(s) 483, 741, 924
RseI CAYNNNNRTG 2 cut(s) 185, 1058
SaqAI TTAA 4 cut(s) 123, 798, 2022, 2067
SatI GCNGC 6 cut(s) 384, 563, 614, 759, 836, 1096
Sau96I GGNCC 4 cut(s) 198, 199, 235, 1602
ScaI AGTACT 4 cut(s) 851, 990, 1364, 1835
SchI GAGTC 6 cut(s) 278, 496, 1148, 1410, 1916, 1979
ScrFI CCNGG 4 cut(s) 196, 203, 1090, 1944
SduI GDGCHC 4 cut(s) 202, 713, 1555, 1967
SfaNI GCATC 4 cut(s) 532, 634, 792, 1465
SfcI CTRYAG 3 cut(s) 654, 1502, 1837
SfiI GGCCNNNNNGGCC 1 cut(s) 197
Sfr274I CTCGAG 1 cut(s) 1223
SfuI TTCGAA 1 cut(s) 1179
SinI GGWCC 1 cut(s) 1602
SlaI CTCGAG 1 cut(s) 1223
SmiMI CAYNNNNRTG 2 cut(s) 185, 1058
SmlI CTYRAG 9 cut(s) 58, 154, 338, 377, 853, 1154, 1170, 1223, 2021
SmoI CTYRAG 9 cut(s) 58, 154, 338, 377, 853, 1154, 1170, 1223, 2021
Sse9I AATT 9 cut(s) 583, 703, 723, 898, 973, 1278, 1332, 1351, 1654
SseBI AGGCCT 1 cut(s) 521
SsiI CCGC 6 cut(s) 383, 739, 835, 1058, 1310, 2051
SspI AATATT 1 cut(s) 1998
SspMI CTAG 6 cut(s) 44, 755, 1127, 1619, 2037, 2085
StuI AGGCCT 1 cut(s) 521
StyD4I CCNGG 4 cut(s) 194, 201, 1088, 1942
StyI CCWWGG 3 cut(s) 186, 1143, 1392
TaaI ACNGT 8 cut(s) 177, 365, 866, 1116, 1527, 1885, 1910, 2060
TaiI ACGT 3 cut(s) 149, 360, 1583
TasI AATT 9 cut(s) 583, 703, 723, 898, 973, 1278, 1332, 1351, 1654
TatI WGTACW 5 cut(s) 477, 849, 988, 1362, 1833
TauI GCSGC 2 cut(s) 386, 838
TfiI GAWTC 9 cut(s) 20, 97, 213, 1071, 1212, 1237, 1435, 1517, 1676
Tru1I TTAA 4 cut(s) 123, 798, 2022, 2067
Tru9I TTAA 4 cut(s) 123, 798, 2022, 2067
TscAI CASTG 4 cut(s) 718, 951, 960, 2104
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 4 cut(s) 562, 613, 758, 1095
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 7 cut(s) 27, 341, 684, 1373, 1589, 1661, 1811
TspRI CASTG 4 cut(s) 718, 951, 960, 2104
Van91I CCANNNNNTGG 1 cut(s) 1492
Vha464I CTTAAG 1 cut(s) 2021
VpaK11BI GGWCC 1 cut(s) 1602
XapI RAATTY 3 cut(s) 583, 898, 973
XceI RCATGY 2 cut(s) 1135, 1690
XhoI CTCGAG 1 cut(s) 1223
XmnI GAANNNNTTC 1 cut(s) 974
XspI CTAG 6 cut(s) 44, 755, 1127, 1619, 2037, 2085
ZrmI AGTACT 4 cut(s) 851, 990, 1364, 1835
Zsp2I ATGCAT 1 cut(s) 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.