MD08G1095500.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005255
Physical Location & Seq
Reverse (-)
7989311 .. 7997500
8190 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1095500.v1.1.491

Sequence Viewer

Length: 3120 bp
ATGGAAGCAGCAGGGGAGGATTGCTGCGTCAAAGTGGCGGTGCACATCCGGCCGCTCATCGGTGACGAGAAGCTTCAGGGGTGTAAAGACTGCGTCACTGTCGTCCCTGGGAAGCCTCAGGTACAAATTGGCACGCATTCGTTTACTTTCGATAATGTGTATGGGAGTACCGGTTCCCCCTCATCTGCAATGTTTGAAGAACGTATCGCTCCACTGGTTGATGGTTTGTTTCATGGATATAATGCTACCGTCCTAGCTTATGGTCAGACGGGTTCTGGAAAAACTTATACCATGGGCACTGGTTTTAGAGATGGTTGCCAATCGGGAATTATCCCACAAGTTATGAATGTATTGTTCAGCAAAATTGAAACTTTGAAGCATCAAACTGAATTCCAGTTGCATGTGTCTTTTATTGAGATTCTAAAAGAAGAAGTACGAGACTTGCTCGATCCTAGTTTTTTGAGCAAACCAGAAGGTGCAAACGGGCATGTGAAAGCAGCATCCCCTGGAAAGCCACCAATACAAATTCGAGAATCATCAAATGGTGTTATTACTCTGGCTGGATCCACTGAACTCAGTGTTAGTACACTAAAAGAAATGGCTGCTTGCCTTGAACAAGGATCGTTGAGCAGGGCAACAGGGAGTACAAATATGAACAATCAATCAAGTCGTTCACATGCCATCTTCACCATTACATTAGAGCAAATGCATAAGGTCAACTCGGCATGTAATGGAAACAGCGGTCTTGATGGGAGTATGAACGAAGAATATCTGTGCGCCAAGTTGCATTTAGTAGATCTTGCTGGGTCTGAGCGAGCCAAGAGGACAGGTTCTGATGGCATGCGTTTTAAGGAAGGAGTTCATATTAATAAGGGTCTTCTTGCCCTTGGGAATGTTATCAGTGCACTTGGTGATGAGAAGAAGCGCAAAGAAGGTCTTCATGTGCCTTATCGAGATAGTAAACTTACTCGGCTTTTGCAGGATTCGCTTGGTGGTAACAGCCGAACTGTTATGATAGCCTGTATCAGTCCTGCTGATATCAATGCTGAGGAAACCCTGAACACGTTAAAGTATGCAAATCGAGCTCGCAATATCCAAAATAAGCCTATTGTAAATAGAGATCCCATGTCCAACGAGATGCTAAAGATGCGCCAACAGCTAGAGTTTTTGCAAGCTGAACTTTGTTCACGTGGAGGAGGATCTTCTTCTGATGAAGTACAGGTTCTCAAGGAAAGAATTACTTGGCTTGAAGCTGCTAATGAGGATCTTTGCCGGGAACTTCATGAATACCGCAGTAAATGCAATGGTGTAGAGCAGTGTGAAAGAGCTTCTCAATATGTGGATGGTAGCCCCTGCTCTGTAAAAAGTGATGGCCTAAAAAGGGGTTTGCAAAGTTTAGAATCGGCAGACTATCAAATGGGTGAAGCAATAACAGGAGATTCCCAGGACATTGATGAAGAAGTAGCAAAAGAGTGGGAGCACAACCTTCTGCAAAATACCATGGACAAAGAGCTGCATGAATTGAATAAACGTCTACAGCAAAAAGAGTCAGAGATGAAATTTTTTGAAGGTTCTGACACTGAGGCACTCAAGCAGCATTTTGGAAAGAAAATTTTGGAATTAGAAGATGAGAAAAGAACTGTGCAGCAAGATAGAGACCGTTTGCTCGGTGAAGTTGAAAATCTTTCTGCTGGTGATGGACAACCACAAAAGTTGCAAGATGTTCATACCCAGAAGTTAAAGGCACTTGAGGGACAGATTTCGGATCTTAAGAAGAAACAAGAGAACCAGGTTCAACTTTTGAAGCAAAAACAAAAAAGTGATGAAGCAGCAAAGCGGCTGCAAGATGAAATCCAGTCTATAAAGGCACAAAAGGTTCAACTGCAACAAAGAATAAAACAAGAAGCAGAACAATTTCGGCAGTGGAAAGCCTCTCGAGAGAAGGAAGTGCTGCAGCTACGGAAAGAAGGCAGGAGAAATGAATATGAAAGACATAAGCTGCAAGCGTTAAATCAACGCCAGAAAATGGTTCTTCAAAGAAAGACTGAAGAGGCTGCAATGGCTACCAAGAGGCTGAAAGAATTGCTAGAAGCTCGTAAATCTTCTGCTCGTGACAGCTCAGTTGTTGCCAATGGAAATGGGACACATGTACAGGGCAATGAGAAATCCCTGCAACGGTGGCTTGATCATGAGCTTGAAGTTATGGTGAATGTACATGAAGTTCGTCATGAATATGAGAAACAAAGTCAAGTACGAGCTGCACTGGCGGAAGAGTTGGCCATGTTGAAGCAATTAAACGAATTTGCTTCGAAGGGCCTTAGTCCTCCAAGAGGAAAGAATGGCTTTGCCCGAGCATCCTCCATGTCACCTAATGCACGAATGGCCAGAATATCATCACTTGAGAACATGCTTAGCATAACATCGAACTCAATAGTAGCAATGGCATCACAACTTTCAGAGGCAGAAGAACGGGAGCGTGCCTTTACCAACCGTGGGCGTTGGAGTCAATTGCGCTCAATGGCAGATGCAAAGAACTTGCTTCAGTATATGTTCAATTCTCTTGCAGATACAAGGTGCCAATTGTGGGAGAAAGAAATGGAAATCGATGAAATGAAAGAGCATCTCAAAGAACTCGTAGGTTTGTTGCGGCAGAGTGAGACACGAAGAAAGGAAGTTGAGAAGGAACTGAAGGTGAGAGAGCAAGAGGTTGCAACTGCATTGGCAACAGCTGCCTCGGCTGTCAATGATCAGGAGAACTCACGCAATTCACTGAAACACTTTGCTGATGACATGAATGGTCCCTTGTCGCCAATCTCCGTGCCAGCACAAAAACAGCTGAAATATACAGCAGGCATTGCCAATGGCTCAGTCAGAGAATCGATAGCTTTCATAGATAAGACACGAAAGATGGTACCCATCGGGCATTTGCCAGCAAAAAAAGTCGCAGTTATAGGGAAAGCTGGGAAGCTATGGAGGTGGAAAAGGAGTCATCACCAGTGGTTAGTCCAATTCAAATGGAAATGGCAGAAGCCTTGGAGACTCTCGGAATGGATTAGGCACAGCGATGAAACTATCATCAGGTCCAAACCTCGCTTACAAGCTCGGTCAAATGTGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000022 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000819 GO:0000910 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003676 GO:0003677 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005829 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005876 GO:0005881 GO:0005938 GO:0006355 GO:0006810 GO:0006890 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0008088 GO:0008089 GO:0008150 GO:0008574 GO:0009664 GO:0009832 GO:0009889 GO:0009891 GO:0009893 GO:0009937 GO:0009966 GO:0009987 GO:0010215 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010970 GO:0015630 GO:0016043 GO:0016192 GO:0016363 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019882 GO:0019884 GO:0019886 GO:0022402 GO:0022607 GO:0023051 GO:0030198 GO:0030496 GO:0030705 GO:0030863 GO:0030981 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032991 GO:0034399 GO:0042127 GO:0042546 GO:0042623 GO:0043062 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044085 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045171 GO:0045229 GO:0045893 GO:0045935 GO:0046907 GO:0048002 GO:0048193 GO:0048285 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051179 GO:0051225 GO:0051231 GO:0051234 GO:0051252 GO:0051254 GO:0051255 GO:0051256 GO:0051276 GO:0051301 GO:0051641 GO:0051649 GO:0055028 GO:0060255 GO:0061640 GO:0065007 GO:0070013 GO:0070726 GO:0070925 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071840 GO:0071944 GO:0080090 GO:0090307 GO:0097159 GO:0098813 GO:0098930 GO:0099080 GO:0099081 GO:0099111 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1901363 GO:1902680 GO:1902850 GO:1903047 GO:1903506 GO:1903508 GO:1990939 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kinesin

Protein Analysis

1040

Amino Acids

116.98

Weight (kDa)

8.77

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 17 - 365 7.4e-103 Kinesin motor domain
Microtub_bd PF16796 40 - 149 6.7e-19 Microtubule binding
KIF21A_4th PF25764 480 - 656 7.9e-56 KIF21A-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2911
AccB1I GGYRCC 2 cut(s) 2574, 2911
AccB7I CCANNNNNTGG 1 cut(s) 2026
AccBSI CCGCTC 1 cut(s) 55
AccI GTMKAC 1 cut(s) 1534
AciI CCGC 7 cut(s) 38, 53, 741, 1291, 1837, 2267, 2647
AclWI GGATC 8 cut(s) 443, 558, 571, 628, 1115, 1207, 1272, 1773
AcoI YGGCCR 3 cut(s) 50, 2277, 2382
AcsI RAATTY 5 cut(s) 389, 525, 1559, 1611, 2300
AcuI CTGAAG 4 cut(s) 59, 2067, 2525, 2708
AcvI CACGTG 1 cut(s) 1190
AdeI CACNNNGTG 1 cut(s) 911
AfiI CCNNNNNNNGG 7 cut(s) 59, 1381, 2026, 2175, 2330, 2493, 2584
AflII CTTAAG 1 cut(s) 1769
AflIII ACRYGT 2 cut(s) 1062, 2146
AgeI ACCGGT 1 cut(s) 170
AjnI CCWGG 4 cut(s) 106, 505, 1443, 1788
AloI GAACNNNNNNTCC 2 cut(s) 157, 189
Alw21I GWGCWC 4 cut(s) 45, 907, 1087, 1482
Alw26I GTCTC 4 cut(s) 432, 1650, 2651, 3031
Alw44I GTGCAC 2 cut(s) 41, 903
AlwI GGATC 8 cut(s) 443, 558, 571, 628, 1115, 1207, 1272, 1773
AlwNI CAGNNNCTG 1 cut(s) 833
Ama87I CYCGRG 2 cut(s) 1935, 2349
AoxI GGCC 5 cut(s) 50, 1372, 2277, 2314, 2382
ApaLI GTGCAC 2 cut(s) 41, 903
ApoI RAATTY 5 cut(s) 389, 525, 1559, 1611, 2300
AseI ATTAAT 1 cut(s) 867
AsiGI ACCGGT 1 cut(s) 170
Asp700I GAANNNNTTC 3 cut(s) 858, 936, 1914
Asp718I GGTACC 1 cut(s) 2911
AspLEI GCGC 4 cut(s) 779, 927, 1152, 2514
AspS9I GGNCC 3 cut(s) 2314, 2798, 3081
AsuC2I CCSGG 1 cut(s) 1274
AsuII TTCGAA 1 cut(s) 2309
AvaI CYCGRG 2 cut(s) 1935, 2349
AvaII GGWCC 2 cut(s) 2798, 3081
AxyI CCTNAGG 1 cut(s) 117
BaeGI GKGCMC 3 cut(s) 45, 299, 907
BalI TGGCCA 2 cut(s) 2279, 2384
BamHI GGATCC 1 cut(s) 563
BanI GGYRCC 2 cut(s) 2574, 2911
BanII GRGCYC 1 cut(s) 1087
BauI CACGAG 1 cut(s) 2109
BbrPI CACGTG 1 cut(s) 1190
BbsI GAAGAC 2 cut(s) 869, 929
Bbv12I GWGCWC 4 cut(s) 45, 907, 1087, 1482
BbvCI CCTCAGC 1 cut(s) 1047
BciT130I CCWGG 4 cut(s) 108, 507, 1445, 1790
BclI TGATCA 2 cut(s) 2185, 2746
BcnI CCSGG 1 cut(s) 1274
BcoDI GTCTC 4 cut(s) 432, 1650, 2651, 3031
BfaI CTAG 4 cut(s) 254, 453, 1160, 2087
BfmI CTRYAG 2 cut(s) 1535, 1952
BfrI CTTAAG 1 cut(s) 1769
BglII AGATCT 1 cut(s) 796
BlpI GCTNAGC 1 cut(s) 2411
Bme1390I CCNGG 5 cut(s) 108, 507, 1274, 1445, 1790
Bme18I GGWCC 2 cut(s) 2798, 3081
BmeT110I CYCGRG 2 cut(s) 1935, 2349
BmgT120I GGNCC 3 cut(s) 2314, 2798, 3081
BmiI GGNNCC 5 cut(s) 175, 565, 2576, 2800, 2913
BmrFI CCNGG 5 cut(s) 108, 507, 1274, 1445, 1790
BmsI GCATC 8 cut(s) 388, 509, 1128, 1137, 2363, 2453, 2515, 2629
BpiI GAAGAC 2 cut(s) 869, 929
BplI GAGNNNNNCTC 2 cut(s) 429, 461
Bpu10I CCTNAGC 1 cut(s) 1047
Bpu1102I GCTNAGC 1 cut(s) 2411
Bpu14I TTCGAA 1 cut(s) 2309
BpuEI CTTGAG 4 cut(s) 1211, 1574, 1769, 2420
BpuMI CCSGG 1 cut(s) 1274
Bsa29I ATCGAT 2 cut(s) 2604, 2879
BsaAI YACGTR 1 cut(s) 1190
BsaI GGTCTC 1 cut(s) 1650
BsaWI WCCGGW 1 cut(s) 170
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 7 cut(s) 59, 1381, 2026, 2175, 2330, 2493, 2584
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 6 cut(s) 219, 304, 394, 1855, 2268, 2995
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 6 cut(s) 195, 1309, 2064, 2164, 2445, 2853
BseBI CCWGG 4 cut(s) 108, 507, 1445, 1790
BseCI ATCGAT 2 cut(s) 2604, 2879
BseGI GGATG 4 cut(s) 45, 500, 1348, 2354
BseLI CCNNNNNNNGG 7 cut(s) 59, 1381, 2026, 2175, 2330, 2493, 2584
BseMI GCAATG 6 cut(s) 195, 1309, 2064, 2164, 2445, 2853
BseMII CTCAG 7 cut(s) 131, 589, 801, 1038, 1572, 2133, 2880
BseNI ACTGG 6 cut(s) 219, 304, 394, 1855, 2268, 2995
BseRI GAGGAG 1 cut(s) 1209
BseSI GKGCMC 3 cut(s) 45, 299, 907
BseX3I CGGCCG 1 cut(s) 50
BseYI CCCAGC 2 cut(s) 803, 2960
BsgI GTGCAG 2 cut(s) 1664, 2244
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 5 cut(s) 52, 1374, 2279, 2316, 2384
BshNI GGYRCC 2 cut(s) 2574, 2911
BshTI ACCGGT 1 cut(s) 170
BshVI ATCGAT 2 cut(s) 2604, 2879
BsiEI CGRYCG 1 cut(s) 53
BsiHKAI GWGCWC 4 cut(s) 45, 907, 1087, 1482
BsiHKCI CYCGRG 2 cut(s) 1935, 2349
BsiSI CCGG 3 cut(s) 49, 171, 1273
BslFI GGGAC 4 cut(s) 89, 1767, 2155, 2784
BslI CCNNNNNNNGG 7 cut(s) 59, 1381, 2026, 2175, 2330, 2493, 2584
BsmAI GTCTC 4 cut(s) 432, 1650, 2651, 3031
BsmFI GGGAC 4 cut(s) 89, 1767, 2155, 2784
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 5 cut(s) 52, 1374, 2279, 2316, 2384
Bso31I GGTCTC 1 cut(s) 1650
BsoBI CYCGRG 2 cut(s) 1935, 2349
Bsp119I TTCGAA 1 cut(s) 2309
Bsp1286I GDGCHC 5 cut(s) 45, 299, 907, 1087, 1482
Bsp1407I TGTACA 2 cut(s) 2149, 2212
Bsp1720I GCTNAGC 1 cut(s) 2411
Bsp19I CCATGG 2 cut(s) 291, 1500
BspACI CCGC 7 cut(s) 38, 53, 741, 1291, 1837, 2267, 2647
BspANI GGCC 5 cut(s) 52, 1374, 2279, 2316, 2384
BspCNI CTCAG 7 cut(s) 130, 588, 802, 1039, 1573, 2132, 2879
BspDI ATCGAT 2 cut(s) 2604, 2879
BspHI TCATGA 3 cut(s) 1282, 2188, 2227
BspLI GGNNCC 5 cut(s) 175, 565, 2576, 2800, 2913
BspMAI CTGCAG 1 cut(s) 1956
BspPI GGATC 8 cut(s) 443, 558, 571, 628, 1115, 1207, 1272, 1773
BspT104I TTCGAA 1 cut(s) 2309
BspT107I GGYRCC 2 cut(s) 2574, 2911
BspTI CTTAAG 1 cut(s) 1769
BspTNI GGTCTC 1 cut(s) 1650
BsrBI CCGCTC 1 cut(s) 55
BsrDI GCAATG 6 cut(s) 195, 1309, 2064, 2164, 2445, 2853
BsrFI RCCGGY 1 cut(s) 170
BsrGI TGTACA 2 cut(s) 2149, 2212
BsrI ACTGG 6 cut(s) 219, 304, 394, 1855, 2268, 2995
BssAI RCCGGY 1 cut(s) 170
BssSI CACGAG 1 cut(s) 2109
BssT1I CCWWGG 4 cut(s) 291, 886, 1500, 3032
Bst2BI CACGAG 1 cut(s) 2109
Bst2UI CCWGG 4 cut(s) 108, 507, 1445, 1790
Bst4CI ACNGT 7 cut(s) 100, 250, 1009, 1642, 1661, 2178, 2492
Bst6I CTCTTC 2 cut(s) 2043, 2265
BstAFI CTTAAG 1 cut(s) 1769
BstAPI GCANNNNNTGC 3 cut(s) 1299, 2729, 2855
BstAUI TGTACA 2 cut(s) 2149, 2212
BstBAI YACGTR 1 cut(s) 1190
BstBI TTCGAA 1 cut(s) 2309
BstDEI CTNAG 9 cut(s) 117, 575, 810, 1047, 1581, 2119, 2318, 2411, 2866
BstDSI CCRYGG 3 cut(s) 291, 1500, 2491
BstENI CCTNNNNNAGG 1 cut(s) 2328
BstF5I GGATG 4 cut(s) 45, 500, 1348, 2354
BstHHI GCGC 4 cut(s) 779, 927, 1152, 2514
BstMAI GTCTC 4 cut(s) 432, 1650, 2651, 3031
BstMCI CGRYCG 1 cut(s) 53
BstNI CCWGG 4 cut(s) 108, 507, 1445, 1790
BstNSI RCATGY 7 cut(s) 404, 491, 680, 729, 844, 2150, 2410
BstSCI CCNGG 5 cut(s) 106, 505, 1272, 1443, 1788
BstSFI CTRYAG 2 cut(s) 1535, 1952
BstSLI GKGCMC 3 cut(s) 45, 299, 907
BstV2I GAAGAC 2 cut(s) 869, 929
BstX2I RGATCY 6 cut(s) 563, 796, 1120, 1199, 1264, 1765
BstYI RGATCY 6 cut(s) 563, 796, 1120, 1199, 1264, 1765
BstZI CGGCCG 1 cut(s) 50
Bsu15I ATCGAT 2 cut(s) 2604, 2879
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 5 cut(s) 52, 1374, 2279, 2316, 2384
BsuTUI ATCGAT 2 cut(s) 2604, 2879
BtgI CCRYGG 3 cut(s) 291, 1500, 2491
BtgZI GCGATG 1 cut(s) 3078
BtsCI GGATG 4 cut(s) 45, 500, 1348, 2354
BtsI GCAGTG 2 cut(s) 1322, 1928
CaiI CAGNNNCTG 1 cut(s) 833
CciI TCATGA 3 cut(s) 1282, 2188, 2227
CfoI GCGC 4 cut(s) 779, 927, 1152, 2514
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 3 cut(s) 2314, 2798, 3081
ClaI ATCGAT 2 cut(s) 2604, 2879
CseI GACGC 2 cut(s) 16, 82
CsiI ACCWGGT 1 cut(s) 1788
CspAI ACCGGT 1 cut(s) 170
CspCI CAANNNNNGTGG 2 cut(s) 1695, 1730
DdeI CTNAG 9 cut(s) 117, 575, 810, 1047, 1581, 2119, 2318, 2411, 2866
DraIII CACNNNGTG 1 cut(s) 911
EaeI YGGCCR 3 cut(s) 50, 2277, 2382
EagI CGGCCG 1 cut(s) 50
Eam1104I CTCTTC 2 cut(s) 2043, 2265
EarI CTCTTC 2 cut(s) 2043, 2265
EciI GGCGGA 1 cut(s) 2282
Ecl136II GAGCTC 1 cut(s) 1085
EclXI CGGCCG 1 cut(s) 50
Eco130I CCWWGG 4 cut(s) 291, 886, 1500, 3032
Eco24I GRGCYC 1 cut(s) 1087
Eco31I GGTCTC 1 cut(s) 1650
Eco32I GATATC 1 cut(s) 1039
Eco47I GGWCC 2 cut(s) 2798, 3081
Eco52I CGGCCG 1 cut(s) 50
Eco53kI GAGCTC 1 cut(s) 1085
Eco57I CTGAAG 4 cut(s) 59, 2067, 2525, 2708
Eco72I CACGTG 1 cut(s) 1190
Eco81I CCTNAGG 1 cut(s) 117
Eco88I CYCGRG 2 cut(s) 1935, 2349
EcoICRI GAGCTC 1 cut(s) 1085
EcoNI CCTNNNNNAGG 1 cut(s) 2328
EcoO109I RGGNCCY 1 cut(s) 2314
EcoRI GAATTC 1 cut(s) 389
EcoRII CCWGG 4 cut(s) 106, 505, 1443, 1788
EcoRV GATATC 1 cut(s) 1039
EcoT14I CCWWGG 4 cut(s) 291, 886, 1500, 3032
EcoT22I ATGCAT 1 cut(s) 711
EcoT38I GRGCYC 1 cut(s) 1087
ErhI CCWWGG 4 cut(s) 291, 886, 1500, 3032
FalI AAGNNNNNCTT 8 cut(s) 921, 953, 1164, 1196, 1225, 1257, 2327, 2359
FaqI GGGAC 4 cut(s) 89, 1767, 2155, 2784
FbaI TGATCA 2 cut(s) 2185, 2746
FblI GTMKAC 1 cut(s) 1534
FokI GGATG 4 cut(s) 32, 487, 1355, 2341
FriOI GRGCYC 1 cut(s) 1087
FspBI CTAG 4 cut(s) 254, 453, 1160, 2087
GlaI GCGC 4 cut(s) 778, 926, 1151, 2513
GsaI CCCAGC 2 cut(s) 807, 2964
HaeIII GGCC 5 cut(s) 52, 1374, 2279, 2316, 2384
HapII CCGG 3 cut(s) 49, 171, 1273
HgaI GACGC 2 cut(s) 16, 82
HhaI GCGC 4 cut(s) 779, 927, 1152, 2514
Hin6I GCGC 4 cut(s) 777, 925, 1150, 2512
HinP1I GCGC 4 cut(s) 777, 925, 1150, 2512
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HindIII AAGCTT 1 cut(s) 71
HpaII CCGG 3 cut(s) 49, 171, 1273
Hpy166II GTNNAC 9 cut(s) 43, 144, 587, 674, 718, 905, 962, 1187, 1535
Hpy8I GTNNAC 9 cut(s) 43, 144, 587, 674, 718, 905, 962, 1187, 1535
HpyCH4III ACNGT 7 cut(s) 100, 250, 1009, 1642, 1661, 2178, 2492
HpyCH4IV ACGT 4 cut(s) 202, 1064, 1189, 1531
HpyF3I CTNAG 9 cut(s) 117, 575, 810, 1047, 1581, 2119, 2318, 2411, 2866
HpySE526I ACGT 4 cut(s) 202, 1064, 1189, 1531
HspAI GCGC 4 cut(s) 777, 925, 1150, 2512
KpnI GGTACC 1 cut(s) 2915
Ksp22I TGATCA 2 cut(s) 2185, 2746
LmnI GCTCC 3 cut(s) 214, 1477, 2473
LweI GCATC 8 cut(s) 388, 509, 1128, 1137, 2363, 2453, 2515, 2629
MabI ACCWGGT 1 cut(s) 1788
MaeI CTAG 4 cut(s) 254, 453, 1160, 2087
MaeII ACGT 4 cut(s) 202, 1064, 1189, 1531
MaeIII GTNAC 5 cut(s) 62, 94, 995, 2111, 2364
MbiI CCGCTC 1 cut(s) 55
MfeI CAATTG 2 cut(s) 2507, 2579
MflI RGATCY 6 cut(s) 563, 796, 1120, 1199, 1264, 1765
MhlI GDGCHC 5 cut(s) 45, 299, 907, 1087, 1482
MlsI TGGCCA 2 cut(s) 2279, 2384
MluNI TGGCCA 2 cut(s) 2279, 2384
MlyI GAGTC 4 cut(s) 1556, 2512, 2995, 3033
MmeI TCCRAC 2 cut(s) 1155, 2480
Mox20I TGGCCA 2 cut(s) 2279, 2384
Mph1103I ATGCAT 1 cut(s) 711
MroXI GAANNNNTTC 3 cut(s) 858, 936, 1914
MscI TGGCCA 2 cut(s) 2279, 2384
MseI TTAA 7 cut(s) 849, 867, 1067, 1739, 1770, 2009, 2294
MslI CAYNNNNRTG 2 cut(s) 2232, 3063
Msp20I TGGCCA 2 cut(s) 2279, 2384
MspA1I CMGCKG 3 cut(s) 741, 2729, 2836
MspCI CTTAAG 1 cut(s) 1769
MspI CCGG 3 cut(s) 49, 171, 1273
MspR9I CCNGG 5 cut(s) 108, 507, 1274, 1445, 1790
MunI CAATTG 2 cut(s) 2507, 2579
Mva1269I GAATGC 1 cut(s) 136
MvaI CCWGG 4 cut(s) 108, 507, 1445, 1790
NciI CCSGG 1 cut(s) 1274
NcoI CCATGG 2 cut(s) 291, 1500
NlaIV GGNNCC 5 cut(s) 175, 565, 2576, 2800, 2913
NmeAIII GCCGAG 3 cut(s) 701, 949, 2714
NmuCI GTSAC 4 cut(s) 62, 94, 2111, 2364
NsiI ATGCAT 1 cut(s) 711
NspI RCATGY 7 cut(s) 404, 491, 680, 729, 844, 2150, 2410
NspV TTCGAA 1 cut(s) 2309
PaeI GCATGC 1 cut(s) 844
PaeR7I CTCGAG 1 cut(s) 1935
PagI TCATGA 3 cut(s) 1282, 2188, 2227
PasI CCCWGGG 1 cut(s) 107
PciI ACATGT 1 cut(s) 2146
PctI GAATGC 1 cut(s) 136
PdmI GAANNNNTTC 3 cut(s) 858, 936, 1914
PfeI GAWTC 6 cut(s) 418, 533, 983, 1400, 1439, 2876
PflFI GACNNNGTC 1 cut(s) 92
PflMI CCANNNNNTGG 1 cut(s) 2026
PinAI ACCGGT 1 cut(s) 170
PleI GAGTC 4 cut(s) 1555, 2511, 2994, 3033
PmaCI CACGTG 1 cut(s) 1190
PmlI CACGTG 1 cut(s) 1190
PpsI GAGTC 4 cut(s) 1555, 2511, 2994, 3033
Ppu21I YACGTR 1 cut(s) 1190
PscI ACATGT 1 cut(s) 2146
PshBI ATTAAT 1 cut(s) 867
Psp124BI GAGCTC 1 cut(s) 1087
Psp6I CCWGG 4 cut(s) 106, 505, 1443, 1788
PspCI CACGTG 1 cut(s) 1190
PspFI CCCAGC 2 cut(s) 803, 2960
PspGI CCWGG 4 cut(s) 106, 505, 1443, 1788
PspN4I GGNNCC 5 cut(s) 175, 565, 2576, 2800, 2913
PspPI GGNCC 3 cut(s) 2314, 2798, 3081
PsrI GAACNNNNNNTAC 2 cut(s) 2205, 2237
PstI CTGCAG 1 cut(s) 1956
PstNI CAGNNNCTG 1 cut(s) 833
PsuI RGATCY 6 cut(s) 563, 796, 1120, 1199, 1264, 1765
PsyI GACNNNGTC 1 cut(s) 92
PvuII CAGCTG 2 cut(s) 2729, 2836
RseI CAYNNNNRTG 2 cut(s) 2232, 3063
SacI GAGCTC 1 cut(s) 1087
SaqAI TTAA 7 cut(s) 849, 867, 1067, 1739, 1770, 2009, 2294
Sau96I GGNCC 3 cut(s) 2314, 2798, 3081
SchI GAGTC 4 cut(s) 1556, 2512, 2995, 3033
ScrFI CCNGG 5 cut(s) 108, 507, 1274, 1445, 1790
SduI GDGCHC 5 cut(s) 45, 299, 907, 1087, 1482
SexAI ACCWGGT 1 cut(s) 1788
SfaNI GCATC 8 cut(s) 388, 509, 1128, 1137, 2363, 2453, 2515, 2629
SfcI CTRYAG 2 cut(s) 1535, 1952
Sfr274I CTCGAG 1 cut(s) 1935
SfuI TTCGAA 1 cut(s) 2309
SinI GGWCC 2 cut(s) 2798, 3081
SlaI CTCGAG 1 cut(s) 1935
SmiMI CAYNNNNRTG 2 cut(s) 2232, 3063
SmlI CTYRAG 6 cut(s) 1226, 1589, 1748, 1769, 1935, 2399
SmoI CTYRAG 6 cut(s) 1226, 1589, 1748, 1769, 1935, 2399
SphI GCATGC 1 cut(s) 844
SsiI CCGC 7 cut(s) 38, 53, 741, 1291, 1837, 2267, 2647
SspMI CTAG 4 cut(s) 254, 453, 1160, 2087
SstI GAGCTC 1 cut(s) 1087
StyD4I CCNGG 5 cut(s) 106, 505, 1272, 1443, 1788
StyI CCWWGG 4 cut(s) 291, 886, 1500, 3032
TaaI ACNGT 7 cut(s) 100, 250, 1009, 1642, 1661, 2178, 2492
TaiI ACGT 4 cut(s) 205, 1067, 1192, 1534
TaqII GACCGA 1 cut(s) 3093
TatI WGTACW 5 cut(s) 584, 644, 1216, 2149, 2212
TauI GCSGC 3 cut(s) 55, 1840, 2650
TfiI GAWTC 6 cut(s) 418, 533, 983, 1400, 1439, 2876
Tru1I TTAA 7 cut(s) 849, 867, 1067, 1739, 1770, 2009, 2294
Tru9I TTAA 7 cut(s) 849, 867, 1067, 1739, 1770, 2009, 2294
TseFI GTSAC 4 cut(s) 62, 94, 2111, 2364
Tsp45I GTSAC 4 cut(s) 62, 94, 2111, 2364
TspGWI ACGGA 2 cut(s) 1975, 2806
Tth111I GACNNNGTC 1 cut(s) 92
Van91I CCANNNNNTGG 1 cut(s) 2026
Vha464I CTTAAG 1 cut(s) 1769
VneI GTGCAC 2 cut(s) 41, 903
VpaK11BI GGWCC 2 cut(s) 2798, 3081
VspI ATTAAT 1 cut(s) 867
XagI CCTNNNNNAGG 1 cut(s) 2328
XapI RAATTY 5 cut(s) 389, 525, 1559, 1611, 2300
XceI RCATGY 7 cut(s) 404, 491, 680, 729, 844, 2150, 2410
XhoI CTCGAG 1 cut(s) 1935
XmiI GTMKAC 1 cut(s) 1534
XmnI GAANNNNTTC 3 cut(s) 858, 936, 1914
XspI CTAG 4 cut(s) 254, 453, 1160, 2087
Zsp2I ATGCAT 1 cut(s) 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.