pycom08g07720
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0005255
Physical Location & Seq
Reverse (-)
6219709 .. 6225303
5595 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g07720.10

Sequence Viewer

Length: 2469 bp
ATGAACAATCAATCAAGTCGTTCACATGCCATCTTCACCATTACATTAGAGCAAATGCATAAGGTCAACACGGCATGTAATGGAAACAACGGTCTTAATGAGAGTATGAACGAAGAATATCTATGCGCCAAGTTGCATTTAGTAGATCTTGCTGGGTCTGAGCGAGCCAAGAGGACAGGTTCTGATGGCATGCGTTTTAAGGAAGGAGTTCATATTAATAAGGGTCTTCTTGCCCTTGGGAATGTTATCAGTGCACTTGGTGATGAGAAGAAGCGCAAAGAAGGTCTTCACGTGCCTTATCGAGATAGCAAACTTACTCGGCTTTTGCAGGATTCGCTTGGTGGTAACAGCCGAACTGTTATGATAGCCTGTATCAGTCCTGCTGATATCAATGCTGAGGAAACCCTGAACACGTTAAAGTATGCAAATCGAGCTCGCAATATCCAAAATAAGCCTATCGTAAATAGAGATCCCATGTCCAATGAGATGCTAAAGATGCGCCAACAGCTAGAGTTTTTGCAAGCTGAACTTTGTTCACGTGGAGGAGGATCTTCTTCTGGTGAAGTACAGGTTCTTAAGGAAAGAATTACTTGGCTTGAAGCTGCTAATGAGGATCTTTGCCGGGAACTTCATGAATACCGCAGTAAATGCAATGGTGTGGAGCAGTGTGAAAGAGCTTCTCAATATGATGGTAGCCCCTGCTCTGTAAAAAGTGATGGCCTAAAAAGGGGTTTGCAAAGTTTAGAATCGGCGGACTATCAAATGGGTGAAGCAATAACAGCAGGTGATTCCCAGGACATTGATGAAGAAGTAGCAAAAGAATGGGAGCACAACCTTCTGCAAAATACCATGGACAAAGAGTTGCATGAATTGAATAAACGTCTACAGCAAAAAGAGTCGGAGATGAAATTTTTTGAAGGGTCCGACACTGAGGCACTCAAGCAGCATTTTGGAAAGAAAATTTTGGAATTAGAAGATGAGAAAAGAACTGTGCAGCAAGATAGGGACCGTTTACTTGGTGAAGTTGAAAATCTTTCTGCCGGTGATGGACAACCACAAAAGTTGCAAGATGTTCATACCCAGAAGTTAAGGGCACTTGAGGGACAGATTTCGGATCTTAAGAAGAAACAAGAGAGCCAGGTTCAACTTTTGAAGCAAAAACAAAAAAGTGATGAAGCAGCAAAGCGGCTGCAAGATGAAATCCAGTCTATAAAGGCACAGAAGGTTCAACTGCAACAAAGGATAAAACAAGAAGCAGAACAATTTCGGCAGTGGAAAGCCTCTCGAGAGAAGGAAGTGCTGCAGCTACGGAAAGAAGGCAGGAGAAATGAATATGAAAGACATAAGCTGCAAGCGTTAAATCAACGCCAGAAAATGGTTCTTCAAAGAAAGACTGAAGAGGCTGCAATGGCTACCAAGAGGCTGAAAGAATTGCTAGAAGCTCGTAAATCTTCTGCTCGTGACAGCTCAGTTGTTGCCAATGGAAATGGGACACATGTACAGGGCAATGAGAAATCCCTGCAACGGTGGCTTGATCATGAGCTTGAAGTTATGGTGAATGTACATGAAGTTCGTCATGAATATGAGAAACAAAGTCAAGTACGAGCTGCACTGGCAGAAGAGTTGGCCATGTTGAAGCAATTAAACGAATTTGCTTCAAAGGGCCTTAGTCCTCCAAGAGGAAAGAATGGCTTTGCCCGAGCATCCTCCATGTCACCTAACGCACGAATGGCCAGAATATTGTCACTTGAGAACATGCTTAGCATAACATCAAACTCAATAGTAGCAATGGCATCACAACTTTCAGAGGCAGAAGAACGGGAGCGTGCCTTTACCAACCGTGGGCGTTGGACTCAATTGCGCTCAATGGCAGATGCAAAGAACTTGCTTCAATATATGTTCAATTCTCTTGCAGATACAAGGTGCCAATTGTGGGAGAAAGAAATGGAAATCGATGAAATGAAGGAGCATCTCAAAGAACTCGTAGGTTTGTTGCGGCAGAGTGAGACACGAAGAAAGGAAGTTGAGAAGGAACTGAAGGTGAGAGAGCAAGAGGTTGCAACTGCATTGGCAACAGCTGCCTCGGCTGTCCATGATCAGGAGAACTCACACAATTCACTGAAACACTTTGCTGATGACACGAATGGTCCCTTGTCCCCAATCTCCGTGCCAGCACAAAAACAGCTGAAATATACAGCAGGCATTGCCAACGGCTCAGTCAGAGAATCGATAGCTTTCATAGATAAGACACGAAAGATGGTACCCATCGGGCATTTGCCAGCGAAAAAAGTCGCAGTTATAGGGAAATCTGGGAAGCTATGGAGGTGGAAGAGGAGTCATCACCAGTGGTTAGTCCAATTCAAATGGAAATGGCAGAAGCCTTGGAGACTCTCGGAATGGATTAGGCACAGCGATGAAACTATCATCAGGTCCAAACCTCGCTTACAAGCTCGGTCAAATGTGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000022 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000819 GO:0000910 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003676 GO:0003677 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005829 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005876 GO:0005881 GO:0005938 GO:0006355 GO:0006810 GO:0006890 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0008088 GO:0008089 GO:0008150 GO:0008574 GO:0009664 GO:0009832 GO:0009889 GO:0009891 GO:0009893 GO:0009937 GO:0009966 GO:0009987 GO:0010215 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010970 GO:0015630 GO:0016043 GO:0016192 GO:0016363 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019882 GO:0019884 GO:0019886 GO:0022402 GO:0022607 GO:0023051 GO:0030198 GO:0030496 GO:0030705 GO:0030863 GO:0030981 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032991 GO:0034399 GO:0042127 GO:0042546 GO:0042623 GO:0043062 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044085 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045171 GO:0045229 GO:0045893 GO:0045935 GO:0046907 GO:0048002 GO:0048193 GO:0048285 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051179 GO:0051225 GO:0051231 GO:0051234 GO:0051252 GO:0051254 GO:0051255 GO:0051256 GO:0051276 GO:0051301 GO:0051641 GO:0051649 GO:0055028 GO:0060255 GO:0061640 GO:0065007 GO:0070013 GO:0070726 GO:0070925 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071840 GO:0071944 GO:0080090 GO:0090307 GO:0097159 GO:0098813 GO:0098930 GO:0099080 GO:0099081 GO:0099111 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1901363 GO:1902680 GO:1902850 GO:1903047 GO:1903506 GO:1903508 GO:1990939 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kinesin

Protein Analysis

823

Amino Acids

93.97

Weight (kDa)

9.17

Isoelectric Point (pI)

50.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 773
Acc36I ACCTGC 1 cut(s) 773
Acc65I GGTACC 1 cut(s) 2260
AccB1I GGYRCC 2 cut(s) 1923, 2260
AccB7I CCANNNNNTGG 1 cut(s) 1375
AccI GTMKAC 1 cut(s) 883
AciI CCGC 4 cut(s) 640, 752, 1186, 1996
AclWI GGATC 4 cut(s) 464, 556, 621, 1122
AcoI YGGCCR 2 cut(s) 1626, 1731
AcsI RAATTY 3 cut(s) 908, 960, 1649
AcuI CTGAAG 2 cut(s) 1416, 2057
AcvI CACGTG 2 cut(s) 292, 539
AdeI CACNNNGTG 1 cut(s) 260
AfaI GTAC 5 cut(s) 567, 1500, 1563, 1602, 2262
AfiI CCNNNNNNNGG 7 cut(s) 727, 1375, 1524, 1679, 1842, 1933, 2098
AflII CTTAAG 2 cut(s) 575, 1118
AflIII ACRYGT 2 cut(s) 411, 1495
AjnI CCWGG 2 cut(s) 792, 1137
Alw21I GWGCWC 3 cut(s) 256, 436, 831
Alw26I GTCTC 2 cut(s) 2000, 2380
Alw44I GTGCAC 1 cut(s) 252
AlwI GGATC 4 cut(s) 464, 556, 621, 1122
AlwNI CAGNNNCTG 1 cut(s) 182
Ama87I CYCGRG 2 cut(s) 1284, 1698
AoxI GGCC 4 cut(s) 718, 1626, 1663, 1731
ApaLI GTGCAC 1 cut(s) 252
ApoI RAATTY 3 cut(s) 908, 960, 1649
AseI ATTAAT 1 cut(s) 216
Asp700I GAANNNNTTC 3 cut(s) 207, 285, 1263
Asp718I GGTACC 1 cut(s) 2260
AspLEI GCGC 4 cut(s) 128, 276, 501, 1863
AspS9I GGNCC 5 cut(s) 921, 1006, 1663, 2147, 2430
AsuC2I CCSGG 1 cut(s) 623
AvaI CYCGRG 2 cut(s) 1284, 1698
AvaII GGWCC 4 cut(s) 921, 1006, 2147, 2430
BaeGI GKGCMC 2 cut(s) 256, 1096
BalI TGGCCA 2 cut(s) 1628, 1733
BanI GGYRCC 2 cut(s) 1923, 2260
BanII GRGCYC 1 cut(s) 436
BauI CACGAG 1 cut(s) 1458
BbrPI CACGTG 2 cut(s) 292, 539
BbsI GAAGAC 2 cut(s) 218, 278
Bbv12I GWGCWC 3 cut(s) 256, 436, 831
BbvCI CCTCAGC 1 cut(s) 396
BccI CCATC 7 cut(s) 38, 179, 683, 710, 1040, 2251, 2273
BceAI ACGGC 2 cut(s) 87, 2227
BciT130I CCWGG 2 cut(s) 794, 1139
BclI TGATCA 2 cut(s) 1534, 2095
BcnI CCSGG 1 cut(s) 623
BcoDI GTCTC 2 cut(s) 2000, 2380
BfaI CTAG 2 cut(s) 509, 1436
BfmI CTRYAG 2 cut(s) 884, 1301
BfrI CTTAAG 2 cut(s) 575, 1118
BfuAI ACCTGC 1 cut(s) 773
BglII AGATCT 1 cut(s) 145
BlpI GCTNAGC 1 cut(s) 1760
Bme1390I CCNGG 3 cut(s) 623, 794, 1139
Bme18I GGWCC 4 cut(s) 921, 1006, 2147, 2430
BmeT110I CYCGRG 2 cut(s) 1284, 1698
BmgT120I GGNCC 5 cut(s) 921, 1006, 1663, 2147, 2430
BmiI GGNNCC 5 cut(s) 922, 1007, 1925, 2149, 2262
BmrFI CCNGG 3 cut(s) 623, 794, 1139
BmsI GCATC 6 cut(s) 477, 486, 1712, 1802, 1864, 1978
BpiI GAAGAC 2 cut(s) 218, 278
Bpu10I CCTNAGC 1 cut(s) 396
Bpu1102I GCTNAGC 1 cut(s) 1760
BpuEI CTTGAG 3 cut(s) 923, 1118, 1769
BpuMI CCSGG 1 cut(s) 623
Bsa29I ATCGAT 2 cut(s) 1953, 2228
BsaAI YACGTR 2 cut(s) 292, 539
BsaJI CCNNGG 6 cut(s) 235, 792, 849, 1840, 2082, 2381
Bsc4I CCNNNNNNNGG 7 cut(s) 727, 1375, 1524, 1679, 1842, 1933, 2098
Bse118I RCCGGY 1 cut(s) 1040
Bse1I ACTGG 3 cut(s) 1204, 1617, 2344
Bse3DI GCAATG 5 cut(s) 658, 1413, 1513, 1794, 2202
BseBI CCWGG 2 cut(s) 794, 1139
BseCI ATCGAT 2 cut(s) 1953, 2228
BseDI CCNNGG 6 cut(s) 235, 792, 849, 1840, 2082, 2381
BseGI GGATG 1 cut(s) 1703
BseLI CCNNNNNNNGG 7 cut(s) 727, 1375, 1524, 1679, 1842, 1933, 2098
BseMI GCAATG 5 cut(s) 658, 1413, 1513, 1794, 2202
BseMII CTCAG 5 cut(s) 150, 387, 921, 1482, 2229
BseNI ACTGG 3 cut(s) 1204, 1617, 2344
BseRI GAGGAG 2 cut(s) 558, 2347
BseSI GKGCMC 2 cut(s) 256, 1096
BseYI CCCAGC 1 cut(s) 152
BsgI GTGCAG 2 cut(s) 1013, 1593
BshFI GGCC 4 cut(s) 720, 1628, 1665, 1733
BshNI GGYRCC 2 cut(s) 1923, 2260
BshVI ATCGAT 2 cut(s) 1953, 2228
BsiHKAI GWGCWC 3 cut(s) 256, 436, 831
BsiHKCI CYCGRG 2 cut(s) 1284, 1698
BsiSI CCGG 2 cut(s) 622, 1041
BslFI GGGAC 5 cut(s) 1019, 1116, 1504, 2133, 2140
BslI CCNNNNNNNGG 7 cut(s) 727, 1375, 1524, 1679, 1842, 1933, 2098
BsmAI GTCTC 2 cut(s) 2000, 2380
BsmFI GGGAC 5 cut(s) 1019, 1116, 1504, 2133, 2140
BsnI GGCC 4 cut(s) 720, 1628, 1665, 1733
BsoBI CYCGRG 2 cut(s) 1284, 1698
Bsp1286I GDGCHC 4 cut(s) 256, 436, 831, 1096
Bsp1407I TGTACA 2 cut(s) 1498, 1561
Bsp143I GATC 7 cut(s) 145, 469, 548, 613, 1114, 1534, 2095
Bsp1720I GCTNAGC 1 cut(s) 1760
Bsp19I CCATGG 1 cut(s) 849
BspACI CCGC 4 cut(s) 640, 752, 1186, 1996
BspANI GGCC 4 cut(s) 720, 1628, 1665, 1733
BspCNI CTCAG 5 cut(s) 151, 388, 922, 1481, 2228
BspDI ATCGAT 2 cut(s) 1953, 2228
BspHI TCATGA 3 cut(s) 631, 1537, 1576
BspLI GGNNCC 5 cut(s) 922, 1007, 1925, 2149, 2262
BspMAI CTGCAG 1 cut(s) 1305
BspMI ACCTGC 1 cut(s) 773
BspPI GGATC 4 cut(s) 464, 556, 621, 1122
BspT107I GGYRCC 2 cut(s) 1923, 2260
BspTI CTTAAG 2 cut(s) 575, 1118
BsrDI GCAATG 5 cut(s) 658, 1413, 1513, 1794, 2202
BsrFI RCCGGY 1 cut(s) 1040
BsrGI TGTACA 2 cut(s) 1498, 1561
BsrI ACTGG 3 cut(s) 1204, 1617, 2344
BssAI RCCGGY 1 cut(s) 1040
BssECI CCNNGG 6 cut(s) 235, 792, 849, 1840, 2082, 2381
BssMI GATC 7 cut(s) 145, 469, 548, 613, 1114, 1534, 2095
BssSI CACGAG 1 cut(s) 1458
BssT1I CCWWGG 3 cut(s) 235, 849, 2381
Bst2BI CACGAG 1 cut(s) 1458
Bst2UI CCWGG 2 cut(s) 794, 1139
Bst4CI ACNGT 6 cut(s) 92, 358, 991, 1010, 1527, 1841
Bst6I CTCTTC 3 cut(s) 1392, 1614, 2324
BstAFI CTTAAG 2 cut(s) 575, 1118
BstAPI GCANNNNNTGC 3 cut(s) 648, 2078, 2204
BstAUI TGTACA 2 cut(s) 1498, 1561
BstBAI YACGTR 2 cut(s) 292, 539
BstC8I GCNNGC 9 cut(s) 165, 191, 436, 522, 1353, 1827, 2172, 2200, 2280
BstDEI CTNAG 7 cut(s) 159, 396, 930, 1468, 1667, 1760, 2215
BstDSI CCRYGG 2 cut(s) 849, 1840
BstENI CCTNNNNNAGG 1 cut(s) 1677
BstF5I GGATG 1 cut(s) 1703
BstHHI GCGC 4 cut(s) 128, 276, 501, 1863
BstKTI GATC 7 cut(s) 148, 472, 551, 616, 1117, 1537, 2098
BstMAI GTCTC 2 cut(s) 2000, 2380
BstMBI GATC 7 cut(s) 145, 469, 548, 613, 1114, 1534, 2095
BstNI CCWGG 2 cut(s) 794, 1139
BstNSI RCATGY 5 cut(s) 29, 78, 193, 1499, 1759
BstSCI CCNGG 3 cut(s) 621, 792, 1137
BstSFI CTRYAG 2 cut(s) 884, 1301
BstSLI GKGCMC 2 cut(s) 256, 1096
BstV2I GAAGAC 2 cut(s) 218, 278
BstX2I RGATCY 5 cut(s) 145, 469, 548, 613, 1114
BstYI RGATCY 5 cut(s) 145, 469, 548, 613, 1114
Bsu15I ATCGAT 2 cut(s) 1953, 2228
BsuRI GGCC 4 cut(s) 720, 1628, 1665, 1733
BsuTUI ATCGAT 2 cut(s) 1953, 2228
BtgI CCRYGG 2 cut(s) 849, 1840
BtgZI GCGATG 1 cut(s) 2427
BtsCI GGATG 1 cut(s) 1703
BtsI GCAGTG 2 cut(s) 671, 1277
BtsIMutI CAGTG 7 cut(s) 256, 671, 927, 1277, 1610, 2117, 2351
BveI ACCTGC 1 cut(s) 773
Cac8I GCNNGC 9 cut(s) 165, 191, 436, 522, 1353, 1827, 2172, 2200, 2280
CaiI CAGNNNCTG 1 cut(s) 182
CciI TCATGA 3 cut(s) 631, 1537, 1576
CfoI GCGC 4 cut(s) 128, 276, 501, 1863
Cfr10I RCCGGY 1 cut(s) 1040
Cfr13I GGNCC 5 cut(s) 921, 1006, 1663, 2147, 2430
ClaI ATCGAT 2 cut(s) 1953, 2228
Csp6I GTAC 5 cut(s) 566, 1499, 1562, 1601, 2261
CspCI CAANNNNNGTGG 2 cut(s) 1044, 1079
CviQI GTAC 5 cut(s) 566, 1499, 1562, 1601, 2261
DdeI CTNAG 7 cut(s) 159, 396, 930, 1468, 1667, 1760, 2215
DpnI GATC 7 cut(s) 147, 471, 550, 615, 1116, 1536, 2097
DpnII GATC 7 cut(s) 145, 469, 548, 613, 1114, 1534, 2095
DraIII CACNNNGTG 1 cut(s) 260
EaeI YGGCCR 2 cut(s) 1626, 1731
Eam1104I CTCTTC 3 cut(s) 1392, 1614, 2324
EarI CTCTTC 3 cut(s) 1392, 1614, 2324
EciI GGCGGA 1 cut(s) 767
Ecl136II GAGCTC 1 cut(s) 434
Eco130I CCWWGG 3 cut(s) 235, 849, 2381
Eco24I GRGCYC 1 cut(s) 436
Eco32I GATATC 1 cut(s) 388
Eco47I GGWCC 4 cut(s) 921, 1006, 2147, 2430
Eco53kI GAGCTC 1 cut(s) 434
Eco57I CTGAAG 2 cut(s) 1416, 2057
Eco72I CACGTG 2 cut(s) 292, 539
Eco88I CYCGRG 2 cut(s) 1284, 1698
EcoICRI GAGCTC 1 cut(s) 434
EcoNI CCTNNNNNAGG 1 cut(s) 1677
EcoO109I RGGNCCY 1 cut(s) 1663
EcoRII CCWGG 2 cut(s) 792, 1137
EcoRV GATATC 1 cut(s) 388
EcoT14I CCWWGG 3 cut(s) 235, 849, 2381
EcoT22I ATGCAT 1 cut(s) 60
EcoT38I GRGCYC 1 cut(s) 436
ErhI CCWWGG 3 cut(s) 235, 849, 2381
FalI AAGNNNNNCTT 8 cut(s) 270, 302, 513, 545, 574, 606, 1676, 1708
FaqI GGGAC 5 cut(s) 1019, 1116, 1504, 2133, 2140
FbaI TGATCA 2 cut(s) 1534, 2095
FblI GTMKAC 1 cut(s) 883
FokI GGATG 1 cut(s) 1690
FriOI GRGCYC 1 cut(s) 436
FspBI CTAG 2 cut(s) 509, 1436
GlaI GCGC 4 cut(s) 127, 275, 500, 1862
GsaI CCCAGC 1 cut(s) 156
HaeIII GGCC 4 cut(s) 720, 1628, 1665, 1733
HapII CCGG 2 cut(s) 622, 1041
HhaI GCGC 4 cut(s) 128, 276, 501, 1863
Hin6I GCGC 4 cut(s) 126, 274, 499, 1861
HinP1I GCGC 4 cut(s) 126, 274, 499, 1861
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 8 cut(s) 332, 746, 788, 896, 1852, 2225, 2335, 2388
HpaII CCGG 2 cut(s) 622, 1041
Hpy166II GTNNAC 6 cut(s) 23, 67, 254, 536, 884, 1013
Hpy188I TCNGA 8 cut(s) 160, 184, 901, 925, 1114, 1807, 2222, 2395
Hpy188III TCNNGA 8 cut(s) 302, 632, 1284, 1286, 1460, 1538, 1577, 2099
Hpy8I GTNNAC 6 cut(s) 23, 67, 254, 536, 884, 1013
HpyCH4III ACNGT 6 cut(s) 92, 358, 991, 1010, 1527, 1841
HpyCH4IV ACGT 4 cut(s) 291, 413, 538, 880
HpyF3I CTNAG 7 cut(s) 159, 396, 930, 1468, 1667, 1760, 2215
HpySE526I ACGT 4 cut(s) 291, 413, 538, 880
HspAI GCGC 4 cut(s) 126, 274, 499, 1861
KpnI GGTACC 1 cut(s) 2264
Ksp22I TGATCA 2 cut(s) 1534, 2095
Kzo9I GATC 7 cut(s) 145, 469, 548, 613, 1114, 1534, 2095
LmnI GCTCC 4 cut(s) 661, 826, 1822, 1966
LweI GCATC 6 cut(s) 477, 486, 1712, 1802, 1864, 1978
MaeI CTAG 2 cut(s) 509, 1436
MaeII ACGT 4 cut(s) 291, 413, 538, 880
MaeIII GTNAC 4 cut(s) 344, 1460, 1713, 1743
MalI GATC 7 cut(s) 147, 471, 550, 615, 1116, 1536, 2097
MboI GATC 7 cut(s) 145, 469, 548, 613, 1114, 1534, 2095
MfeI CAATTG 2 cut(s) 1856, 1928
MflI RGATCY 5 cut(s) 145, 469, 548, 613, 1114
MhlI GDGCHC 4 cut(s) 256, 436, 831, 1096
MlsI TGGCCA 2 cut(s) 1628, 1733
MluNI TGGCCA 2 cut(s) 1628, 1733
MlyI GAGTC 4 cut(s) 905, 1846, 2344, 2382
MmeI TCCRAC 3 cut(s) 879, 948, 1829
Mox20I TGGCCA 2 cut(s) 1628, 1733
Mph1103I ATGCAT 1 cut(s) 60
MroXI GAANNNNTTC 3 cut(s) 207, 285, 1263
MscI TGGCCA 2 cut(s) 1628, 1733
MseI TTAA 9 cut(s) 96, 198, 216, 416, 576, 1088, 1119, 1358, 1643
MslI CAYNNNNRTG 2 cut(s) 1581, 2412
Msp20I TGGCCA 2 cut(s) 1628, 1733
MspA1I CMGCKG 2 cut(s) 2078, 2185
MspCI CTTAAG 2 cut(s) 575, 1118
MspI CCGG 2 cut(s) 622, 1041
MspR9I CCNGG 3 cut(s) 623, 794, 1139
MunI CAATTG 2 cut(s) 1856, 1928
MvaI CCWGG 2 cut(s) 794, 1139
NciI CCSGG 1 cut(s) 623
NcoI CCATGG 1 cut(s) 849
NdeII GATC 7 cut(s) 145, 469, 548, 613, 1114, 1534, 2095
NlaIV GGNNCC 5 cut(s) 922, 1007, 1925, 2149, 2262
NmeAIII GCCGAG 2 cut(s) 298, 2063
NmuCI GTSAC 3 cut(s) 1460, 1713, 1743
NsiI ATGCAT 1 cut(s) 60
NspI RCATGY 5 cut(s) 29, 78, 193, 1499, 1759
PaeI GCATGC 1 cut(s) 193
PaeR7I CTCGAG 1 cut(s) 1284
PagI TCATGA 3 cut(s) 631, 1537, 1576
PaqCI CACCTGC 1 cut(s) 773
PciI ACATGT 1 cut(s) 1495
PdmI GAANNNNTTC 3 cut(s) 207, 285, 1263
PfeI GAWTC 4 cut(s) 332, 746, 788, 2225
PflMI CCANNNNNTGG 1 cut(s) 1375
PleI GAGTC 4 cut(s) 904, 1846, 2343, 2382
PmaCI CACGTG 2 cut(s) 292, 539
PmlI CACGTG 2 cut(s) 292, 539
PpsI GAGTC 4 cut(s) 904, 1846, 2343, 2382
Ppu21I YACGTR 2 cut(s) 292, 539
PscI ACATGT 1 cut(s) 1495
PshBI ATTAAT 1 cut(s) 216
Psp124BI GAGCTC 1 cut(s) 436
Psp6I CCWGG 2 cut(s) 792, 1137
PspCI CACGTG 2 cut(s) 292, 539
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 2 cut(s) 792, 1137
PspN4I GGNNCC 5 cut(s) 922, 1007, 1925, 2149, 2262
PspPI GGNCC 5 cut(s) 921, 1006, 1663, 2147, 2430
PsrI GAACNNNNNNTAC 2 cut(s) 1554, 1586
PstI CTGCAG 1 cut(s) 1305
PstNI CAGNNNCTG 1 cut(s) 182
PsuI RGATCY 5 cut(s) 145, 469, 548, 613, 1114
PvuII CAGCTG 2 cut(s) 2078, 2185
RsaI GTAC 5 cut(s) 567, 1500, 1563, 1602, 2262
RsaNI GTAC 5 cut(s) 566, 1499, 1562, 1601, 2261
RseI CAYNNNNRTG 2 cut(s) 1581, 2412
SacI GAGCTC 1 cut(s) 436
SaqAI TTAA 9 cut(s) 96, 198, 216, 416, 576, 1088, 1119, 1358, 1643
Sau3AI GATC 7 cut(s) 145, 469, 548, 613, 1114, 1534, 2095
Sau96I GGNCC 5 cut(s) 921, 1006, 1663, 2147, 2430
SchI GAGTC 4 cut(s) 905, 1846, 2344, 2382
ScrFI CCNGG 3 cut(s) 623, 794, 1139
SduI GDGCHC 4 cut(s) 256, 436, 831, 1096
SfaNI GCATC 6 cut(s) 477, 486, 1712, 1802, 1864, 1978
SfcI CTRYAG 2 cut(s) 884, 1301
Sfr274I CTCGAG 1 cut(s) 1284
SinI GGWCC 4 cut(s) 921, 1006, 2147, 2430
SlaI CTCGAG 1 cut(s) 1284
SmiMI CAYNNNNRTG 2 cut(s) 1581, 2412
SmlI CTYRAG 6 cut(s) 575, 938, 1097, 1118, 1284, 1748
SmoI CTYRAG 6 cut(s) 575, 938, 1097, 1118, 1284, 1748
SphI GCATGC 1 cut(s) 193
SsiI CCGC 4 cut(s) 640, 752, 1186, 1996
SspI AATATT 1 cut(s) 1740
SspMI CTAG 2 cut(s) 509, 1436
SstI GAGCTC 1 cut(s) 436
StyD4I CCNGG 3 cut(s) 621, 792, 1137
StyI CCWWGG 3 cut(s) 235, 849, 2381
TaaI ACNGT 6 cut(s) 92, 358, 991, 1010, 1527, 1841
TaiI ACGT 4 cut(s) 294, 416, 541, 883
TaqI TCGA 5 cut(s) 301, 430, 1285, 1953, 2228
TaqII GACCGA 1 cut(s) 2442
TatI WGTACW 3 cut(s) 565, 1498, 1561
TauI GCSGC 2 cut(s) 1189, 1999
TfiI GAWTC 4 cut(s) 332, 746, 788, 2225
Tru1I TTAA 9 cut(s) 96, 198, 216, 416, 576, 1088, 1119, 1358, 1643
Tru9I TTAA 9 cut(s) 96, 198, 216, 416, 576, 1088, 1119, 1358, 1643
TscAI CASTG 7 cut(s) 256, 671, 934, 1277, 1617, 2124, 2351
TseFI GTSAC 3 cut(s) 1460, 1713, 1743
Tsp45I GTSAC 3 cut(s) 1460, 1713, 1743
TspGWI ACGGA 2 cut(s) 1324, 2155
TspRI CASTG 7 cut(s) 256, 671, 934, 1277, 1617, 2124, 2351
Van91I CCANNNNNTGG 1 cut(s) 1375
Vha464I CTTAAG 2 cut(s) 575, 1118
VneI GTGCAC 1 cut(s) 252
VpaK11BI GGWCC 4 cut(s) 921, 1006, 2147, 2430
VspI ATTAAT 1 cut(s) 216
XagI CCTNNNNNAGG 1 cut(s) 1677
XapI RAATTY 3 cut(s) 908, 960, 1649
XceI RCATGY 5 cut(s) 29, 78, 193, 1499, 1759
XhoI CTCGAG 1 cut(s) 1284
XmiI GTMKAC 1 cut(s) 883
XmnI GAANNNNTTC 3 cut(s) 207, 285, 1263
XspI CTAG 2 cut(s) 509, 1436
Zsp2I ATGCAT 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.