Rroxscaffold_7G00160120
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005255
Physical Location & Seq
Reverse (-)
3124704 .. 3133434
8731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00160120.1

Sequence Viewer

Length: 3288 bp
ATGGAAGCCGGGGAGGATTGTAGTGTTAAAGTGGCCGTCCACATCCGGCCGCTCGTCGGAGACGAGAAGCTGCAGGGATGCAAAGATTGCGTCACTGTAGTCCCTGGGAAGCCTCAGGTACAAATTGGGACACATTCATTTACTTTTGATCATGTCTACGGCAGTACTGGTTCTCCATCATCTGCAATGTTTGAAGAGTGTATTGTTTCGCTGGTCGATGGTTTGTTCCAAGGATATAATGCTACTGTCCTTGCTTATGGTCAGACCGGTTCAGGGAAAACATATACCATGGGCACTGGATTCAGAGATGGTTTGCAAACTGGAATTATCCCTCAAGTCATGAATGTATTATTCAGCAAAATTGAAACTTTGAAGCATCAAACGGAGTTCCAGTTGCATGTTTCCTTTATTGAGATTCTCAAAGAGGAAGTACGAGACTTGCTGGATCCCAGTTTTCTGAGCAAACCAGAAGGTGCAAATGGGCATGCCGCAAAAGTAGCAATCCCCGGAAAACCACCAATACAAATTCGAGAATCATCAAATGGTGTTATTACATTGGCAGGATCTACTGAAATTAGTGTTAGTACACTTAAACAAATGGCCACATGTCTTGAACAAGGATCACTGAACAGGGCTACAGGGAGTACAAATATGAACAATCAGTCGAGTCGTTCACATGCCATCTTCACCATTACATTAGAGCAAATGCATAAGCTCAACCCAGCATGTTCCGGCAACGGTCTCAATGAGAGTATGAATGAAGATTATCTATGCGCTAAATTGCATTTGGTAGATCTTGCTGGGTCTGAGCGAGCCAAGAGGACAGGTTCTGATGGTTTGCGTTTTAAGGAAGGAGTTCATATTAACAAAGGTCTTCTTGCACTTGGAAATGTTATCAGTGCACTTGGTGATGAGAAGAAGCGAAAAGAAGGACTTCATGTTCCTTATCGAGATAGTAAACTTACTCGGCTTTTGCAGGACTCGCTTGGTGGTAACAGCCGAACTGTTATGATAGCTTGCATCAGTCCTGCTGATATTAATGCTGAGGAAACCTTGAACACTTTGAAATATGCAAATCGTGCTCGAAATATCCAAAATAAGCCCATTGTCAACAGAGATCCCATGTCGAATGAGATGCTGAAGATGCGCCAACAATTAGAGTATTTGCAAGCCGAGCTTTGTGCTCGTGGAGGAGGATCTTCTGATGAAATGCAGGTTCTCAAGGAAAGAATTGCTTGGCTTGAAGCAGCTAATGAGGATCTCTGCCGAGAACTTCATGAATACCGAAAGAAATGCACAGTTGTAGACCAATGTGAAAAAGATGCTCAAGATGCTAGTCCCTGCTCTTTGAAAACTGATGGCCTTAAAAGGGGTTTGCTAAGTATAGAATCAGCTGACTATCAAATGGGTGAAGCAATATCAGGTGATTCTGGGGAAATTGATGAAGAAGTAGCAAAAGAGTGGGAGCACAGCCTTCTGCAAAATACCATGGACAAAGAGTTGCATGAACTGAATAAACGTCTTCAGCAGAAAGAGTCGGAGATGAAATCTTTTGAAGGGTCTGACACGGTGGCACTCAAGCAGCATTTTGGAAAGAAAATAATGGAACTTGAAGATGAGAAAAGAGCTGTGCAGAAAGAGAGGGACCACTTGTTGGGTGAAGTCGAAAATCTTGCTGCTAGTGATGGACAAGCACAGAAACTGCAAGATGTTCATTCCCAAAAGTTAAAGGCACTTGAGGCACAGATTCTGGATCTCAAGAAGAAACAAGAGAGCCAGGTTCAACTTTTGAAGCAAAAACAAAAAAGTGATGAAGCGGCAAAGCGGCTTCAAGATGAAATTCAATCTATAAAGGCACAAAAGGTTCAATTGCAACAAAGGATAAAACAAGAAGCAGAACAATTCCGGCAGTGGAAAGCATCTCGAGAGAAGGAATTGCTGCAGTTACGGAAAGAGGGCAGGAGAAATGAATATGAAAGGCATAAGCTGCAGGCTTTAAATCAGCGCCAAAAAATGGTCCTTCAAAGAAAGACTGAAGAGGCTGCAATGGCTACTAAGAGGCTTAAAGAATTGCTTGAAGCTCGTAAATCTTCTGCTCGTGACAACTCAGTTGTTGCCCATGGGAATGGGTCCAATGGGACACATGGACAGAACAATGAAAAATCCCTGCAACGGTGGCTAGATCATGAGCTGGAAGTCATGGTGAATGTGCATGAAGTTCGTCATGAATATGAGAAACAAAGTCAAGTACGTGCTGCGTTGGCAGAAGAGTTGGCCATGCTGAAGCAAGTAGACGAATTTGCATCAAAGGGCCTTAGTCCTCCAAGAGGAAAGAATGGCTTTTCCAGAGTTTCCTCCATGTCACCAAATGCAAGAATGGCCAGAATATCTTCACTTGAGAACATGCTTGGCATATCATCAAACTCACTTGTAGCAATGGCATCACAACTTTCAGAGGCAGAAGAGCGAGAGCGTGCTTTTACTAACCGTGGACGTTGGAACCAATTGCGCTCAATGGCAGATGCAAAGAACTTGCTTCAATATATGTTCGATTCTGTTGCTGATGCAAGGTGCCAATGTTGGGAGAAGAATATGGAAATCAAGGAAATGAAAGAGCATCTCAAAGAACTTGTAGGCTTGTTGCGGCAGAGTGAAACACGAAGAAAGGAAGTTGAGAAGGAACTAAAATTTAGAGAGCAAGATGCCGCAGCTGCATTGGCAACACCACCATCGGCTGGCTATGATCACGGGAACTCGCACAATTCACTGAAACACTTTGCTGATGACACGAATGGTCCCTTGTCCCCAATCTCTGTGCCTGCACAAAAACAGCTAAAGTATACAGCAGGCATTGCTAATGGCCGAGTGAGAGAATCCACAGCTTTCATAGATCAGACACGAAAGATGGTACCTATCGGCCATTTGCCAACAAAAAAATTAACAGTTATAGGACAATCTGGAAAGCTATGGAGATGGAAGAGGAGCCATCACCAGTGGCTAGTACAATTCAAATGGAAATGGCAAAAGCCATGGAGACTCTCAGAATGGATTAGGCACAGTGATGAAACAATTATAAGGGCAAAGCCTCGGTGTGCTATAAGTAGCTATTCGGATCCTGCTACTGTTGTTCTGGAAAGCATGCATAGGTTTGTTGTTGAAAATTTTGGAATGGTTACTGAACTCGGAGCATCTTGTAAAGCTGTGTTGATGCTGCCGATGATACTTTGTGTAGCTAGAGACCACACCAAAGGCAGTGGCGCAAGACTGCAAGTGGTGTTATACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000022 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000819 GO:0000910 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003676 GO:0003677 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005829 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005876 GO:0005881 GO:0005938 GO:0006355 GO:0006810 GO:0006890 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0008088 GO:0008089 GO:0008150 GO:0008574 GO:0009664 GO:0009832 GO:0009889 GO:0009891 GO:0009893 GO:0009937 GO:0009966 GO:0009987 GO:0010215 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010970 GO:0015630 GO:0016043 GO:0016192 GO:0016363 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019882 GO:0019884 GO:0019886 GO:0022402 GO:0022607 GO:0023051 GO:0030198 GO:0030496 GO:0030705 GO:0030863 GO:0030981 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032991 GO:0034399 GO:0042127 GO:0042546 GO:0042623 GO:0043062 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044085 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045171 GO:0045229 GO:0045893 GO:0045935 GO:0046907 GO:0048002 GO:0048193 GO:0048285 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051179 GO:0051225 GO:0051231 GO:0051234 GO:0051252 GO:0051254 GO:0051255 GO:0051256 GO:0051276 GO:0051301 GO:0051641 GO:0051649 GO:0055028 GO:0060255 GO:0061640 GO:0065007 GO:0070013 GO:0070726 GO:0070925 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071840 GO:0071944 GO:0080090 GO:0090307 GO:0097159 GO:0098813 GO:0098930 GO:0099080 GO:0099081 GO:0099111 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1901363 GO:1902680 GO:1902850 GO:1903047 GO:1903506 GO:1903508 GO:1990939 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kinesin

Protein Analysis

1095

Amino Acids

123.04

Weight (kDa)

8.7

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 16 - 364 2.2e-102 Kinesin motor domain
Microtub_bd PF16796 39 - 148 1.4e-19 Microtubule binding
KIF21A_4th PF25764 475 - 652 1.4e-56 KIF21A-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3074
Acc36I ACCTGC 1 cut(s) 1204
Acc65I GGTACC 1 cut(s) 2908
AccB1I GGYRCC 2 cut(s) 2571, 2908
AccB7I CCANNNNNTGG 3 cut(s) 1654, 2014, 2735
AccBSI CCGCTC 1 cut(s) 52
AccI GTMKAC 4 cut(s) 156, 1305, 2292, 2840
AciI CCGC 6 cut(s) 50, 489, 1817, 1825, 2644, 2706
AcoI YGGCCR 7 cut(s) 33, 47, 600, 2274, 2379, 2860, 2917
AcsI RAATTY 5 cut(s) 525, 1839, 2297, 2687, 3160
AcuI CTGAAG 4 cut(s) 1160, 1508, 2055, 2303
AdeI CACNNNGTG 1 cut(s) 908
AfaI GTAC 8 cut(s) 120, 166, 432, 586, 646, 2250, 2910, 3003
AfiI CCNNNNNNNGG 9 cut(s) 56, 273, 1369, 1654, 2014, 2172, 2327, 2581, 2735
AflIII ACRYGT 1 cut(s) 605
AgeI ACCGGT 1 cut(s) 266
AjnI CCWGG 2 cut(s) 103, 1776
AloI GAACNNNNNNTCC 2 cut(s) 924, 956
Alw21I GWGCWC 4 cut(s) 904, 1084, 1186, 1470
Alw26I GTCTC 5 cut(s) 54, 429, 746, 3028, 3231
Alw44I GTGCAC 1 cut(s) 900
AlwNI CAGNNNCTG 3 cut(s) 830, 1702, 1750
Ama87I CYCGRG 1 cut(s) 1923
AoxI GGCC 9 cut(s) 33, 47, 600, 1360, 2274, 2311, 2379, 2860, 2917
ApaLI GTGCAC 1 cut(s) 900
ApoI RAATTY 5 cut(s) 525, 1839, 2297, 2687, 3160
ArsI GACNNNNNNTTYG 2 cut(s) 75, 107
AseI ATTAAT 1 cut(s) 1038
AsiGI ACCGGT 1 cut(s) 266
Asp700I GAANNNNTTC 3 cut(s) 855, 933, 2389
Asp718I GGTACC 1 cut(s) 2908
AspLEI GCGC 5 cut(s) 776, 1149, 2007, 2511, 3260
AspS9I GGNCC 5 cut(s) 1645, 2017, 2130, 2311, 2795
AsuC2I CCSGG 2 cut(s) 10, 507
AsuHPI GGTGA 8 cut(s) 679, 920, 1421, 1436, 1670, 2215, 2355, 2981
AvaI CYCGRG 1 cut(s) 1923
AvaII GGWCC 4 cut(s) 1645, 2017, 2130, 2795
AxyI CCTNAGG 1 cut(s) 114
BaeGI GKGCMC 2 cut(s) 296, 904
BalI TGGCCA 3 cut(s) 602, 2276, 2381
BamHI GGATCC 2 cut(s) 445, 3112
BanI GGYRCC 2 cut(s) 2571, 2908
BauI CACGAG 2 cut(s) 1185, 2097
BbsI GAAGAC 2 cut(s) 866, 1514
Bbv12I GWGCWC 4 cut(s) 904, 1084, 1186, 1470
BbvCI CCTCAGC 1 cut(s) 1044
BceAI ACGGC 2 cut(s) 20, 175
BciT130I CCWGG 2 cut(s) 105, 1778
BclI TGATCA 2 cut(s) 148, 2743
BcnI CCSGG 2 cut(s) 10, 507
BcoDI GTCTC 5 cut(s) 54, 429, 746, 3028, 3231
BfaI CTAG 5 cut(s) 1335, 1680, 2180, 2999, 3234
BfmI CTRYAG 5 cut(s) 71, 96, 636, 1940, 1988
BfoI RGCGCY 1 cut(s) 2008
BfuAI ACCTGC 1 cut(s) 1204
BglII AGATCT 1 cut(s) 793
BmcAI AGTACT 1 cut(s) 166
Bme1390I CCNGG 4 cut(s) 10, 105, 507, 1778
Bme18I GGWCC 4 cut(s) 1645, 2017, 2130, 2795
BmeT110I CYCGRG 1 cut(s) 1923
BmgT120I GGNCC 5 cut(s) 1645, 2017, 2130, 2311, 2795
BmiI GGNNCC 9 cut(s) 447, 1646, 2131, 2501, 2573, 2797, 2910, 2984, 3114
BmrFI CCNGG 4 cut(s) 10, 105, 507, 1778
BmrI ACTGGG 1 cut(s) 444
BmuI ACTGGG 1 cut(s) 444
BpiI GAAGAC 2 cut(s) 866, 1514
Bpu10I CCTNAGC 1 cut(s) 1044
BpuEI CTTGAG 7 cut(s) 318, 1205, 1311, 1562, 1742, 1757, 2417
BpuMI CCSGG 2 cut(s) 10, 507
BsaAI YACGTR 1 cut(s) 2252
BsaI GGTCTC 2 cut(s) 746, 3231
BsaWI WCCGGW 1 cut(s) 266
BsaXI ACNNNNNCTCC 4 cut(s) 157, 187, 377, 407
Bsc4I CCNNNNNNNGG 9 cut(s) 56, 273, 1369, 1654, 2014, 2172, 2327, 2581, 2735
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 7 cut(s) 172, 301, 325, 391, 450, 2992, 3287
Bse21I CCTNAGG 1 cut(s) 114
Bse3DI GCAATG 4 cut(s) 192, 2052, 2442, 2850
BseBI CCWGG 2 cut(s) 105, 1778
BseGI GGATG 2 cut(s) 42, 83
BseLI CCNNNNNNNGG 9 cut(s) 56, 273, 1369, 1654, 2014, 2172, 2327, 2581, 2735
BseMI GCAATG 4 cut(s) 192, 2052, 2442, 2850
BseMII CTCAG 6 cut(s) 128, 449, 798, 1035, 2121, 3054
BseNI ACTGG 7 cut(s) 172, 301, 325, 391, 450, 2992, 3287
BseRI GAGGAG 2 cut(s) 1206, 2995
BseSI GKGCMC 2 cut(s) 296, 904
BseX3I CGGCCG 1 cut(s) 47
BseYI CCCAGC 2 cut(s) 721, 800
BsgI GTGCAG 2 cut(s) 1652, 2805
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 9 cut(s) 35, 49, 602, 1362, 2276, 2313, 2381, 2862, 2919
BshNI GGYRCC 2 cut(s) 2571, 2908
BshTI ACCGGT 1 cut(s) 266
BsiEI CGRYCG 1 cut(s) 50
BsiHKAI GWGCWC 4 cut(s) 904, 1084, 1186, 1470
BsiHKCI CYCGRG 1 cut(s) 1923
BsiSI CCGG 6 cut(s) 9, 46, 267, 507, 732, 1906
BslFI GGGAC 7 cut(s) 86, 142, 1323, 1658, 2152, 2781, 2788
BslI CCNNNNNNNGG 9 cut(s) 56, 273, 1369, 1654, 2014, 2172, 2327, 2581, 2735
BsmAI GTCTC 5 cut(s) 54, 429, 746, 3028, 3231
BsmBI CGTCTC 1 cut(s) 54
BsmFI GGGAC 7 cut(s) 86, 142, 1323, 1658, 2152, 2781, 2788
BsnI GGCC 9 cut(s) 35, 49, 602, 1362, 2276, 2313, 2381, 2862, 2919
Bso31I GGTCTC 2 cut(s) 746, 3231
BsoBI CYCGRG 1 cut(s) 1923
Bsp1286I GDGCHC 5 cut(s) 296, 904, 1084, 1186, 1470
Bsp19I CCATGG 4 cut(s) 288, 1488, 2119, 3029
BspACI CCGC 6 cut(s) 50, 489, 1817, 1825, 2644, 2706
BspANI GGCC 9 cut(s) 35, 49, 602, 1362, 2276, 2313, 2381, 2862, 2919
BspCNI CTCAG 6 cut(s) 127, 450, 799, 1036, 2120, 3053
BspHI TCATGA 4 cut(s) 339, 1276, 2185, 2224
BspLI GGNNCC 9 cut(s) 447, 1646, 2131, 2501, 2573, 2797, 2910, 2984, 3114
BspMAI CTGCAG 3 cut(s) 75, 1944, 1992
BspMI ACCTGC 1 cut(s) 1204
BspQI GCTCTTC 1 cut(s) 2457
BspT107I GGYRCC 2 cut(s) 2571, 2908
BspTNI GGTCTC 2 cut(s) 746, 3231
BsrBI CCGCTC 1 cut(s) 52
BsrDI GCAATG 4 cut(s) 192, 2052, 2442, 2850
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 7 cut(s) 172, 301, 325, 391, 450, 2992, 3287
BssAI RCCGGY 1 cut(s) 266
BssNAI GTATAC 1 cut(s) 2841
BssSI CACGAG 2 cut(s) 1185, 2097
BssT1I CCWWGG 5 cut(s) 229, 288, 1488, 2119, 3029
Bst1107I GTATAC 1 cut(s) 2841
Bst2BI CACGAG 2 cut(s) 1185, 2097
Bst2UI CCWGG 2 cut(s) 105, 1778
Bst6I CTCTTC 5 cut(s) 189, 2031, 2262, 2457, 2972
BstAPI GCANNNNNTGC 4 cut(s) 87, 1079, 1987, 2852
BstBAI YACGTR 1 cut(s) 2252
BstDEI CTNAG 9 cut(s) 114, 458, 807, 1044, 1379, 2055, 2107, 2315, 3040
BstDSI CCRYGG 5 cut(s) 288, 1488, 2119, 2488, 3029
BstENI CCTNNNNNAGG 1 cut(s) 2325
BstF5I GGATG 2 cut(s) 42, 83
BstH2I RGCGCY 1 cut(s) 2008
BstHHI GCGC 5 cut(s) 776, 1149, 2007, 2511, 3260
BstMAI GTCTC 5 cut(s) 54, 429, 746, 3028, 3231
BstMCI CGRYCG 1 cut(s) 50
BstNI CCWGG 2 cut(s) 105, 1778
BstNSI RCATGY 7 cut(s) 401, 488, 609, 680, 729, 2407, 3142
BstSCI CCNGG 4 cut(s) 8, 103, 505, 1776
BstSFI CTRYAG 5 cut(s) 71, 96, 636, 1940, 1988
BstSLI GKGCMC 2 cut(s) 296, 904
BstV2I GAAGAC 2 cut(s) 866, 1514
BstX2I RGATCY 8 cut(s) 445, 563, 793, 1117, 1196, 1258, 1753, 3112
BstXI CCANNNNNNTGG 1 cut(s) 2126
BstYI RGATCY 8 cut(s) 445, 563, 793, 1117, 1196, 1258, 1753, 3112
BstZ17I GTATAC 1 cut(s) 2841
BstZI CGGCCG 1 cut(s) 47
Bsu36I CCTNAGG 1 cut(s) 114
BsuRI GGCC 9 cut(s) 35, 49, 602, 1362, 2276, 2313, 2381, 2862, 2919
BtgI CCRYGG 5 cut(s) 288, 1488, 2119, 2488, 3029
BtsCI GGATG 2 cut(s) 42, 83
BtsI GCAGTG 2 cut(s) 1916, 3259
BtsIMutI CAGTG 9 cut(s) 93, 294, 623, 904, 1916, 2765, 2999, 3064, 3259
BveI ACCTGC 1 cut(s) 1204
CaiI CAGNNNCTG 3 cut(s) 830, 1702, 1750
CciI TCATGA 4 cut(s) 339, 1276, 2185, 2224
CfoI GCGC 5 cut(s) 776, 1149, 2007, 2511, 3260
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 5 cut(s) 1645, 2017, 2130, 2311, 2795
CseI GACGC 1 cut(s) 79
Csp6I GTAC 8 cut(s) 119, 165, 431, 585, 645, 2249, 2909, 3002
CspAI ACCGGT 1 cut(s) 266
CspCI CAANNNNNGTGG 4 cut(s) 592, 627, 3235, 3270
CviQI GTAC 8 cut(s) 119, 165, 431, 585, 645, 2249, 2909, 3002
DdeI CTNAG 9 cut(s) 114, 458, 807, 1044, 1379, 2055, 2107, 2315, 3040
DraI TTTAAA 1 cut(s) 1998
DraIII CACNNNGTG 1 cut(s) 908
EaeI YGGCCR 7 cut(s) 33, 47, 600, 2274, 2379, 2860, 2917
EagI CGGCCG 1 cut(s) 47
Eam1104I CTCTTC 5 cut(s) 189, 2031, 2262, 2457, 2972
EarI CTCTTC 5 cut(s) 189, 2031, 2262, 2457, 2972
EclXI CGGCCG 1 cut(s) 47
Eco130I CCWWGG 5 cut(s) 229, 288, 1488, 2119, 3029
Eco31I GGTCTC 2 cut(s) 746, 3231
Eco47I GGWCC 4 cut(s) 1645, 2017, 2130, 2795
Eco52I CGGCCG 1 cut(s) 47
Eco57I CTGAAG 4 cut(s) 1160, 1508, 2055, 2303
Eco81I CCTNAGG 1 cut(s) 114
Eco88I CYCGRG 1 cut(s) 1923
EcoNI CCTNNNNNAGG 1 cut(s) 2325
EcoO109I RGGNCCY 1 cut(s) 2311
EcoRII CCWGG 2 cut(s) 103, 1776
EcoT14I CCWWGG 5 cut(s) 229, 288, 1488, 2119, 3029
EcoT22I ATGCAT 2 cut(s) 711, 3144
ErhI CCWWGG 5 cut(s) 229, 288, 1488, 2119, 3029
Esp3I CGTCTC 1 cut(s) 54
FaqI GGGAC 7 cut(s) 86, 142, 1323, 1658, 2152, 2781, 2788
FbaI TGATCA 2 cut(s) 148, 2743
FblI GTMKAC 4 cut(s) 156, 1305, 2292, 2840
FokI GGATG 2 cut(s) 29, 90
FspBI CTAG 5 cut(s) 1335, 1680, 2180, 2999, 3234
GlaI GCGC 5 cut(s) 775, 1148, 2006, 2510, 3259
GsaI CCCAGC 2 cut(s) 725, 804
HaeII RGCGCY 1 cut(s) 2008
HaeIII GGCC 9 cut(s) 35, 49, 602, 1362, 2276, 2313, 2381, 2862, 2919
HapII CCGG 6 cut(s) 9, 46, 267, 507, 732, 1906
HgaI GACGC 1 cut(s) 79
HhaI GCGC 5 cut(s) 776, 1149, 2007, 2511, 3260
Hin6I GCGC 5 cut(s) 774, 1147, 2005, 2509, 3258
HinP1I GCGC 5 cut(s) 774, 1147, 2005, 2509, 3258
HincII GTYRAC 1 cut(s) 1111
HindII GTYRAC 1 cut(s) 1111
HpaII CCGG 6 cut(s) 9, 46, 267, 507, 732, 1906
HphI GGTGA 8 cut(s) 679, 920, 1421, 1436, 1670, 2215, 2355, 2981
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 8 cut(s) 464, 845, 923, 1484, 1550, 1924, 2030, 2671
HpyCH4IV ACGT 3 cut(s) 1519, 2251, 2494
HpyF3I CTNAG 9 cut(s) 114, 458, 807, 1044, 1379, 2055, 2107, 2315, 3040
HpySE526I ACGT 3 cut(s) 1519, 2251, 2494
HspAI GCGC 5 cut(s) 774, 1147, 2005, 2509, 3258
KpnI GGTACC 1 cut(s) 2912
Ksp22I TGATCA 2 cut(s) 148, 2743
LguI GCTCTTC 1 cut(s) 2457
LmnI GCTCC 3 cut(s) 1465, 2982, 3185
MaeI CTAG 5 cut(s) 1335, 1680, 2180, 2999, 3234
MaeII ACGT 3 cut(s) 1519, 2251, 2494
MaeIII GTNAC 6 cut(s) 91, 992, 1944, 2099, 2361, 3172
MbiI CCGCTC 1 cut(s) 52
MfeI CAATTG 2 cut(s) 1868, 2504
MflI RGATCY 8 cut(s) 445, 563, 793, 1117, 1196, 1258, 1753, 3112
MhlI GDGCHC 5 cut(s) 296, 904, 1084, 1186, 1470
MlsI TGGCCA 3 cut(s) 602, 2276, 2381
MluNI TGGCCA 3 cut(s) 602, 2276, 2381
MlyI GAGTC 4 cut(s) 676, 974, 1544, 3030
MmeI TCCRAC 3 cut(s) 37, 1518, 2477
Mox20I TGGCCA 3 cut(s) 602, 2276, 2381
Mph1103I ATGCAT 2 cut(s) 711, 3144
MroXI GAANNNNTTC 3 cut(s) 855, 933, 2389
MscI TGGCCA 3 cut(s) 602, 2276, 2381
MslI CAYNNNNRTG 3 cut(s) 2124, 2229, 3060
Msp20I TGGCCA 3 cut(s) 602, 2276, 2381
MspA1I CMGCKG 2 cut(s) 1394, 2711
MspI CCGG 6 cut(s) 9, 46, 267, 507, 732, 1906
MspR9I CCNGG 4 cut(s) 10, 105, 507, 1778
MunI CAATTG 2 cut(s) 1868, 2504
MvaI CCWGG 2 cut(s) 105, 1778
NciI CCSGG 2 cut(s) 10, 507
NcoI CCATGG 4 cut(s) 288, 1488, 2119, 3029
NlaIV GGNNCC 9 cut(s) 447, 1646, 2131, 2501, 2573, 2797, 2910, 2984, 3114
NmeAIII GCCGAG 4 cut(s) 946, 1198, 1292, 2888
NmuCI GTSAC 3 cut(s) 91, 2099, 2361
NsiI ATGCAT 2 cut(s) 711, 3144
NspI RCATGY 7 cut(s) 401, 488, 609, 680, 729, 2407, 3142
PaeI GCATGC 2 cut(s) 488, 3142
PaeR7I CTCGAG 1 cut(s) 1923
PagI TCATGA 4 cut(s) 339, 1276, 2185, 2224
PasI CCCWGGG 1 cut(s) 104
PciI ACATGT 1 cut(s) 605
PciSI GCTCTTC 1 cut(s) 2457
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PdmI GAANNNNTTC 3 cut(s) 855, 933, 2389
PfeI GAWTC 8 cut(s) 300, 415, 533, 1388, 1427, 1747, 2552, 2873
PflMI CCANNNNNTGG 3 cut(s) 1654, 2014, 2735
PinAI ACCGGT 1 cut(s) 266
PleI GAGTC 4 cut(s) 675, 974, 1543, 3030
PpsI GAGTC 4 cut(s) 675, 974, 1543, 3030
Ppu21I YACGTR 1 cut(s) 2252
PscI ACATGT 1 cut(s) 605
PshBI ATTAAT 1 cut(s) 1038
PsiI TTATAA 1 cut(s) 3074
Psp6I CCWGG 2 cut(s) 103, 1776
PspFI CCCAGC 2 cut(s) 721, 800
PspGI CCWGG 2 cut(s) 103, 1776
PspN4I GGNNCC 9 cut(s) 447, 1646, 2131, 2501, 2573, 2797, 2910, 2984, 3114
PspPI GGNCC 5 cut(s) 1645, 2017, 2130, 2311, 2795
PstI CTGCAG 3 cut(s) 75, 1944, 1992
PstNI CAGNNNCTG 3 cut(s) 830, 1702, 1750
PsuI RGATCY 8 cut(s) 445, 563, 793, 1117, 1196, 1258, 1753, 3112
PvuII CAGCTG 2 cut(s) 1394, 2711
RsaI GTAC 8 cut(s) 120, 166, 432, 586, 646, 2250, 2910, 3003
RsaNI GTAC 8 cut(s) 119, 165, 431, 585, 645, 2249, 2909, 3002
RseI CAYNNNNRTG 3 cut(s) 2124, 2229, 3060
SapI GCTCTTC 1 cut(s) 2457
Sau96I GGNCC 5 cut(s) 1645, 2017, 2130, 2311, 2795
ScaI AGTACT 1 cut(s) 166
SchI GAGTC 4 cut(s) 676, 974, 1544, 3030
ScrFI CCNGG 4 cut(s) 10, 105, 507, 1778
SduI GDGCHC 5 cut(s) 296, 904, 1084, 1186, 1470
SfcI CTRYAG 5 cut(s) 71, 96, 636, 1940, 1988
Sfr274I CTCGAG 1 cut(s) 1923
SinI GGWCC 4 cut(s) 1645, 2017, 2130, 2795
SlaI CTCGAG 1 cut(s) 1923
SmiMI CAYNNNNRTG 3 cut(s) 2124, 2229, 3060
SmlI CTYRAG 8 cut(s) 333, 1220, 1326, 1577, 1736, 1757, 1923, 2396
SmoI CTYRAG 8 cut(s) 333, 1220, 1326, 1577, 1736, 1757, 1923, 2396
SphI GCATGC 2 cut(s) 488, 3142
SsiI CCGC 6 cut(s) 50, 489, 1817, 1825, 2644, 2706
SspMI CTAG 5 cut(s) 1335, 1680, 2180, 2999, 3234
StyD4I CCNGG 4 cut(s) 8, 103, 505, 1776
StyI CCWWGG 5 cut(s) 229, 288, 1488, 2119, 3029
TaiI ACGT 3 cut(s) 1522, 2254, 2497
TaqI TCGA 9 cut(s) 216, 529, 665, 949, 1084, 1127, 1665, 1924, 2550
TatI WGTACW 4 cut(s) 164, 584, 644, 3001
TauI GCSGC 6 cut(s) 52, 491, 1820, 1828, 2647, 2708
TfiI GAWTC 8 cut(s) 300, 415, 533, 1388, 1427, 1747, 2552, 2873
TscAI CASTG 9 cut(s) 100, 301, 630, 904, 1916, 2772, 2999, 3064, 3259
TseFI GTSAC 3 cut(s) 91, 2099, 2361
Tsp45I GTSAC 3 cut(s) 91, 2099, 2361
TspGWI ACGGA 2 cut(s) 398, 1963
TspRI CASTG 9 cut(s) 100, 301, 630, 904, 1916, 2772, 2999, 3064, 3259
Van91I CCANNNNNTGG 3 cut(s) 1654, 2014, 2735
VneI GTGCAC 1 cut(s) 900
VpaK11BI GGWCC 4 cut(s) 1645, 2017, 2130, 2795
VspI ATTAAT 1 cut(s) 1038
XagI CCTNNNNNAGG 1 cut(s) 2325
XapI RAATTY 5 cut(s) 525, 1839, 2297, 2687, 3160
XceI RCATGY 7 cut(s) 401, 488, 609, 680, 729, 2407, 3142
XhoI CTCGAG 1 cut(s) 1923
XmiI GTMKAC 4 cut(s) 156, 1305, 2292, 2840
XmnI GAANNNNTTC 3 cut(s) 855, 933, 2389
XspI CTAG 5 cut(s) 1335, 1680, 2180, 2999, 3234
ZrmI AGTACT 1 cut(s) 166
Zsp2I ATGCAT 2 cut(s) 711, 3144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.