Rw6G041570
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0005255
Physical Location & Seq
Forward (+)
65230937 .. 65239569
8633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G041570.1

Sequence Viewer

Length: 3123 bp
ATGGAAGCCGGGGAGGATTGTAGTGTTAAAGTGGCCGTCCACATCCGGCCGCTCGTCGGAGACGAGAAGCTGCAGGGATGTAAAGATTGCGTCACTGTAGTCCCTGGGAAGCCTCAGGTACAAATTGGGACACATTCATTTACTTTTGATCATGTCTACGGCAGTACTGGTTCTCCATCATCTGCAATGTTTGAAGAGTGTATTGTTTCGCTGGTCGATGGTTTGTTCCAAGGATATAATGCTACTGTCCTTGCTTATGGTCAGACCGGTTCAGGGAAAACATATACCATGGGCACTGGTTTCAGAGATGGTTTGCAAACTGGAATTATCCCTCAAGTCATGAATGTATTATTCAGCAAAATTGAAACTTTGAAGCATCAAACGGAGTTCCAGTTGCATGTTTCCTTTATTGAGATTCTCAAAGAGGAAGTACGAGACTTGCTGGATCCCAGTTTTCTGAGCAAACCAGAAGGTGCAAATGGTCATGCTGCAAAAGTAGCAATCCCCGGAAAACCACCAATACAAATTCGAGAATCATCAAATGGTGTTATTACATTGGCAGGATCTACTGAAATTAGTGTTAGTACACTTAAACAAATGGCCACATGTCTTGAACAAGGATCACTGAACAGGGCTACAGGGAGTACAAATATGAACAATCAGTCAAGTCGTTCACATGCCATCTTCACCATTACATTAGAGCAAATGCATAAGCTCAACCCAGCATGTTCCGGCAACGGTCTCAATGAGAGTATGAATGAAGATTATCTATGCGCTAAATTGCATTTGGTAGATCTTGCTGGGTCTGAGCGAGCCAAGAGGACAGGTTCTGATGGTTTGCGTTTTAAGGAAGGAGTTCATATTAACAAAGGTCTTCTTGCACTTGGAAATGTTATCAGTGCACTTGGTGATGAGAAGAAGCGAAAAGAAGGACTTCATGTTCCTTATCGAGATAGTAAACTTACTCGGCTTTTGCAGGACTCGCTTGGTGGTAACAGCCGAACTGTTATGATAGCTTGCATCAGTCCTGCTGATATTAATGCTGAGGAAACCTTGAACACTTTGAAATATGCAAATCGTGCTCGAAATATCCAAAATAAGCCCATTGTGAACAGAGATCCCATGTCGAATGAAATGCTGAAGATGCGCCAACAATTAGAGTATTTGCAAGCCGAGCTTTGTGCTCGTGGAGGAGGATCTTCTGATGAAATGCAGGTTCTCAAGGAAAGAATTGCTTGGCTTGAAGCAGCTAATGAGGATCTCTGCCGAGAACTTCATGAATACCGAAAGAAATGCACAGTTGTAGACCAATGTGAAAAAGATGCTCAAGATGCTAGTCCCTGCTCTTTGAAAACTGATGGCCTTAAAAGGGGTTTGCTAAGTATAGAATCGGCTGACTATCAAATGGGTGAAGCAATTTCAGCAGGTGATTCTGGGGAAATTGATGAAGAAGTAGCAAAAGAGTGGGAGCACAGCCTTCTGCAAAATACCATGGACAAAGAGTTGCATGAACTGAATAAACGTCTTCAGCAGAAAGAGTCGGAGATGAAATCTTTTGAAGGGTCTGACACGGTGGCACTCAAGCAGCATTTTGGAAAGAAAATAATGGAACTTGAAGATGAGAAAAGAGCTGTGCAGAAAGAGAGGGACCACTTGTTGGGTGAAGTCGAAAATCTTGCTGCTAGTGATGGACAAGCACAGAAGCTGCAAGATGTTCATTCCCAAAAGTTAAAGGCACTTGAGGCACAGATTCTGGATCTAAAGAAGAAACAAGAGAGCCAGGTTCAACTTTTGAAGCAAAAACAAAAAAGTGATGAAGCAGCAAAGCGGCTTCAAGATGAAATTCAATCTATAAAGGCACAAAAGGTTCAATTGCAACAAAGGATAAAACAAGAAGCAGAACAATTCCGGCAGTGGAAAGCATCTCGAGAGAAGGAATTGCTGCAGTTACGGAAAGAGGGCAGGAGAAATGAATATGAAAGGCATAAGCTGCAGGCTTTAAATCAGCGCCAAAAAATGGTCCTTCAAAGAAAGACTGAAGAGGCTGCAATGGCTACTAAGAGGCTTAAAGAATTGCTTGAAGCTCGTAAATCTTCTGCTCGTGACAACTCAGTTGTTGCCCATGGGAATGGGTCCAATGGGACACATGGACAGAACAATGAAAAATCCCTGCAACGGTGGCTAGATCATGAGCTGGAAGTCATGGTGAATGTGCATGAAGTTCGTCATGAATATGAGAAACAAAGTCAAGTACGTGCTGCATTGGCAGAAGAGTTGGCCATGCTGAAGCAAGTAGACGAATTTGCATCAAAGGGCCTTAGTCCTCCAAGAGGAAAGAATGGCTTTTCCAGAGTTTCCTCCATGTCACCAAATGCAAGAATGGCCAGAATATCTTCACTTGAGAACATGCTTGGCATATCATCAAACTCACTTGTAGCAATGGCATCACAACTTTCAGAGGCAGAAGAGCGAGAGCGTGCTTTTACTAACCGTGGACGTTGGAACCAATTGCGCTCAATGGCAGATGCAAAGAACTTGCTTCAATATATGTTCGATTCTGTTGCTGATGCAAGGTGCCAATGTTGGGAGAAGGATATGGAAATCAAGGAAATGAAAGAGCATCTCAAAGAACTTGTAGGCTTGTTGCGGCAGAGTGAAACACGAAGAAAGGAAGTTGAGAAGGAACTAAAATTTAGAGAGCAAGATGCCGCAGCTGCATTGGCAACACCACCATCGGCTGGCTATGATCACGGGAACTCGCACAATTCACTGAAACACTTTGCTGATGACACGAATGGTCCCTTGTCCCCAATCTCTGTGCCTGCACAAAAACAGCTAAAGTATACAGCAGGCATTGCTAATGGCCGAGTGAGAGAATCCACAGCTTTCATAGATCAGACACGAAAGATGGTACCTATCGGGCATTTGCCAACAAAAAAATTAACAGTTATAGGACAATCTGGAAAGCTATGGAGATGGAAGAGGAGCCATCACCAGTGGCTAGTACAATTCAAATGGAAATGGCAAAAACCTTGGAGACTCTCAGAATGGATTAGGCACAGTGATGAAACCATTATAAGGGCAAAGCCTCGGGTACAAGCACTGTCAAATGGGAGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000022 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000819 GO:0000910 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003676 GO:0003677 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005829 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005876 GO:0005881 GO:0005938 GO:0006355 GO:0006810 GO:0006890 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0008088 GO:0008089 GO:0008150 GO:0008574 GO:0009664 GO:0009832 GO:0009889 GO:0009891 GO:0009893 GO:0009937 GO:0009966 GO:0009987 GO:0010215 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010970 GO:0015630 GO:0016043 GO:0016192 GO:0016363 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019882 GO:0019884 GO:0019886 GO:0022402 GO:0022607 GO:0023051 GO:0030198 GO:0030496 GO:0030705 GO:0030863 GO:0030981 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032991 GO:0034399 GO:0042127 GO:0042546 GO:0042623 GO:0043062 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044085 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045171 GO:0045229 GO:0045893 GO:0045935 GO:0046907 GO:0048002 GO:0048193 GO:0048285 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051179 GO:0051225 GO:0051231 GO:0051234 GO:0051252 GO:0051254 GO:0051255 GO:0051256 GO:0051276 GO:0051301 GO:0051641 GO:0051649 GO:0055028 GO:0060255 GO:0061640 GO:0065007 GO:0070013 GO:0070726 GO:0070925 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071840 GO:0071944 GO:0080090 GO:0090307 GO:0097159 GO:0098813 GO:0098930 GO:0099080 GO:0099081 GO:0099111 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1901363 GO:1902680 GO:1902850 GO:1903047 GO:1903506 GO:1903508 GO:1990939 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kinesin

Protein Analysis

1040

Amino Acids

116.97

Weight (kDa)

8.76

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 16 - 364 2e-102 Kinesin motor domain
Microtub_bd PF16796 39 - 148 1.3e-19 Microtubule binding
KIF21A_4th PF25764 477 - 653 1.5e-56 KIF21A-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3077
AarI CACCTGC 1 cut(s) 1415
Acc36I ACCTGC 2 cut(s) 1204, 1415
Acc65I GGTACC 1 cut(s) 2911
AccB1I GGYRCC 2 cut(s) 2574, 2911
AccB7I CCANNNNNTGG 3 cut(s) 1657, 2017, 2738
AccBSI CCGCTC 1 cut(s) 52
AccI GTMKAC 4 cut(s) 156, 1305, 2295, 2843
AciI CCGC 4 cut(s) 50, 1828, 2647, 2709
AclWI GGATC 8 cut(s) 440, 453, 571, 628, 1112, 1204, 1266, 1764
AcoI YGGCCR 6 cut(s) 33, 47, 600, 2277, 2382, 2863
AcsI RAATTY 4 cut(s) 525, 1842, 2300, 2690
AcuI CTGAAG 4 cut(s) 1160, 1511, 2058, 2306
AdeI CACNNNGTG 1 cut(s) 908
AfaI GTAC 9 cut(s) 120, 166, 432, 586, 646, 2253, 2913, 3006, 3096
AflIII ACRYGT 1 cut(s) 605
AgeI ACCGGT 1 cut(s) 266
AjnI CCWGG 2 cut(s) 103, 1779
AloI GAACNNNNNNTCC 2 cut(s) 924, 956
Alw21I GWGCWC 4 cut(s) 904, 1084, 1186, 1473
Alw26I GTCTC 4 cut(s) 54, 429, 746, 3031
Alw44I GTGCAC 1 cut(s) 900
AlwI GGATC 8 cut(s) 440, 453, 571, 628, 1112, 1204, 1266, 1764
AlwNI CAGNNNCTG 3 cut(s) 830, 1705, 1753
Ama87I CYCGRG 2 cut(s) 1926, 3090
AoxI GGCC 8 cut(s) 33, 47, 600, 1360, 2277, 2314, 2382, 2863
ApaLI GTGCAC 1 cut(s) 900
ApoI RAATTY 4 cut(s) 525, 1842, 2300, 2690
AseI ATTAAT 1 cut(s) 1038
AsiGI ACCGGT 1 cut(s) 266
Asp700I GAANNNNTTC 3 cut(s) 855, 933, 2392
Asp718I GGTACC 1 cut(s) 2911
AspLEI GCGC 4 cut(s) 776, 1149, 2010, 2514
AspS9I GGNCC 5 cut(s) 1648, 2020, 2133, 2314, 2798
AsuC2I CCSGG 2 cut(s) 10, 507
AsuHPI GGTGA 8 cut(s) 679, 920, 1421, 1439, 1673, 2218, 2358, 2984
AvaI CYCGRG 2 cut(s) 1926, 3090
AvaII GGWCC 4 cut(s) 1648, 2020, 2133, 2798
AxyI CCTNAGG 1 cut(s) 114
BaeGI GKGCMC 2 cut(s) 296, 904
BalI TGGCCA 3 cut(s) 602, 2279, 2384
BamHI GGATCC 1 cut(s) 445
BanI GGYRCC 2 cut(s) 2574, 2911
BauI CACGAG 2 cut(s) 1185, 2100
BbsI GAAGAC 2 cut(s) 866, 1517
Bbv12I GWGCWC 4 cut(s) 904, 1084, 1186, 1473
BbvCI CCTCAGC 1 cut(s) 1044
BceAI ACGGC 2 cut(s) 20, 175
BciT130I CCWGG 2 cut(s) 105, 1781
BclI TGATCA 2 cut(s) 148, 2746
BcnI CCSGG 2 cut(s) 10, 507
BcoDI GTCTC 4 cut(s) 54, 429, 746, 3031
BfaI CTAG 4 cut(s) 1335, 1683, 2183, 3002
BfmI CTRYAG 5 cut(s) 71, 96, 636, 1943, 1991
BfoI RGCGCY 1 cut(s) 2011
BfuAI ACCTGC 2 cut(s) 1204, 1415
BglII AGATCT 1 cut(s) 793
BmcAI AGTACT 1 cut(s) 166
Bme1390I CCNGG 4 cut(s) 10, 105, 507, 1781
Bme18I GGWCC 4 cut(s) 1648, 2020, 2133, 2798
BmeT110I CYCGRG 2 cut(s) 1926, 3090
BmgT120I GGNCC 5 cut(s) 1648, 2020, 2133, 2314, 2798
BmiI GGNNCC 8 cut(s) 447, 1649, 2134, 2504, 2576, 2800, 2913, 2987
BmrFI CCNGG 4 cut(s) 10, 105, 507, 1781
BmrI ACTGGG 1 cut(s) 444
BmuI ACTGGG 1 cut(s) 444
BpiI GAAGAC 2 cut(s) 866, 1517
Bpu10I CCTNAGC 1 cut(s) 1044
BpuEI CTTGAG 6 cut(s) 318, 1205, 1311, 1565, 1760, 2420
BpuMI CCSGG 2 cut(s) 10, 507
BsaAI YACGTR 1 cut(s) 2255
BsaI GGTCTC 1 cut(s) 746
BsaWI WCCGGW 1 cut(s) 266
BsaXI ACNNNNNCTCC 4 cut(s) 157, 187, 377, 407
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 6 cut(s) 172, 301, 325, 391, 450, 2995
Bse21I CCTNAGG 1 cut(s) 114
Bse3DI GCAATG 4 cut(s) 192, 2055, 2445, 2853
BseBI CCWGG 2 cut(s) 105, 1781
BseGI GGATG 2 cut(s) 42, 83
BseMI GCAATG 4 cut(s) 192, 2055, 2445, 2853
BseMII CTCAG 6 cut(s) 128, 449, 798, 1035, 2124, 3057
BseNI ACTGG 6 cut(s) 172, 301, 325, 391, 450, 2995
BseRI GAGGAG 2 cut(s) 1206, 2998
BseSI GKGCMC 2 cut(s) 296, 904
BseX3I CGGCCG 1 cut(s) 47
BseYI CCCAGC 2 cut(s) 721, 800
BsgI GTGCAG 2 cut(s) 1655, 2808
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 8 cut(s) 35, 49, 602, 1362, 2279, 2316, 2384, 2865
BshNI GGYRCC 2 cut(s) 2574, 2911
BshTI ACCGGT 1 cut(s) 266
BsiEI CGRYCG 1 cut(s) 50
BsiHKAI GWGCWC 4 cut(s) 904, 1084, 1186, 1473
BsiHKCI CYCGRG 2 cut(s) 1926, 3090
BsiSI CCGG 6 cut(s) 9, 46, 267, 507, 732, 1909
BslFI GGGAC 7 cut(s) 86, 142, 1323, 1661, 2155, 2784, 2791
BsmAI GTCTC 4 cut(s) 54, 429, 746, 3031
BsmBI CGTCTC 1 cut(s) 54
BsmFI GGGAC 7 cut(s) 86, 142, 1323, 1661, 2155, 2784, 2791
BsnI GGCC 8 cut(s) 35, 49, 602, 1362, 2279, 2316, 2384, 2865
Bso31I GGTCTC 1 cut(s) 746
BsoBI CYCGRG 2 cut(s) 1926, 3090
Bsp1286I GDGCHC 5 cut(s) 296, 904, 1084, 1186, 1473
Bsp19I CCATGG 3 cut(s) 288, 1491, 2122
BspACI CCGC 4 cut(s) 50, 1828, 2647, 2709
BspANI GGCC 8 cut(s) 35, 49, 602, 1362, 2279, 2316, 2384, 2865
BspCNI CTCAG 6 cut(s) 127, 450, 799, 1036, 2123, 3056
BspHI TCATGA 4 cut(s) 339, 1276, 2188, 2227
BspLI GGNNCC 8 cut(s) 447, 1649, 2134, 2504, 2576, 2800, 2913, 2987
BspMAI CTGCAG 3 cut(s) 75, 1947, 1995
BspMI ACCTGC 2 cut(s) 1204, 1415
BspPI GGATC 8 cut(s) 440, 453, 571, 628, 1112, 1204, 1266, 1764
BspQI GCTCTTC 1 cut(s) 2460
BspT107I GGYRCC 2 cut(s) 2574, 2911
BspTNI GGTCTC 1 cut(s) 746
BsrBI CCGCTC 1 cut(s) 52
BsrDI GCAATG 4 cut(s) 192, 2055, 2445, 2853
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 6 cut(s) 172, 301, 325, 391, 450, 2995
BssAI RCCGGY 1 cut(s) 266
BssNAI GTATAC 1 cut(s) 2844
BssSI CACGAG 2 cut(s) 1185, 2100
BssT1I CCWWGG 5 cut(s) 229, 288, 1491, 2122, 3032
Bst1107I GTATAC 1 cut(s) 2844
Bst2BI CACGAG 2 cut(s) 1185, 2100
Bst2UI CCWGG 2 cut(s) 105, 1781
Bst6I CTCTTC 5 cut(s) 189, 2034, 2265, 2460, 2975
BstAPI GCANNNNNTGC 3 cut(s) 1079, 1990, 2855
BstBAI YACGTR 1 cut(s) 2255
BstC8I GCNNGC 8 cut(s) 813, 1018, 1170, 1995, 2478, 2740, 2823, 2851
BstDEI CTNAG 9 cut(s) 114, 458, 807, 1044, 1379, 2058, 2110, 2318, 3043
BstDSI CCRYGG 4 cut(s) 288, 1491, 2122, 2491
BstENI CCTNNNNNAGG 1 cut(s) 2328
BstF5I GGATG 2 cut(s) 42, 83
BstH2I RGCGCY 1 cut(s) 2011
BstHHI GCGC 4 cut(s) 776, 1149, 2010, 2514
BstMAI GTCTC 4 cut(s) 54, 429, 746, 3031
BstMCI CGRYCG 1 cut(s) 50
BstNI CCWGG 2 cut(s) 105, 1781
BstNSI RCATGY 5 cut(s) 401, 609, 680, 729, 2410
BstSCI CCNGG 4 cut(s) 8, 103, 505, 1779
BstSFI CTRYAG 5 cut(s) 71, 96, 636, 1943, 1991
BstSLI GKGCMC 2 cut(s) 296, 904
BstV2I GAAGAC 2 cut(s) 866, 1517
BstX2I RGATCY 7 cut(s) 445, 563, 793, 1117, 1196, 1258, 1756
BstXI CCANNNNNNTGG 1 cut(s) 2129
BstYI RGATCY 7 cut(s) 445, 563, 793, 1117, 1196, 1258, 1756
BstZ17I GTATAC 1 cut(s) 2844
BstZI CGGCCG 1 cut(s) 47
Bsu36I CCTNAGG 1 cut(s) 114
BsuRI GGCC 8 cut(s) 35, 49, 602, 1362, 2279, 2316, 2384, 2865
BtgI CCRYGG 4 cut(s) 288, 1491, 2122, 2491
BtsCI GGATG 2 cut(s) 42, 83
BtsI GCAGTG 1 cut(s) 1919
BtsIMutI CAGTG 9 cut(s) 93, 294, 623, 904, 1919, 2768, 3002, 3067, 3101
BveI ACCTGC 2 cut(s) 1204, 1415
Cac8I GCNNGC 8 cut(s) 813, 1018, 1170, 1995, 2478, 2740, 2823, 2851
CaiI CAGNNNCTG 3 cut(s) 830, 1705, 1753
CciI TCATGA 4 cut(s) 339, 1276, 2188, 2227
CfoI GCGC 4 cut(s) 776, 1149, 2010, 2514
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 5 cut(s) 1648, 2020, 2133, 2314, 2798
CseI GACGC 1 cut(s) 79
Csp6I GTAC 9 cut(s) 119, 165, 431, 585, 645, 2252, 2912, 3005, 3095
CspAI ACCGGT 1 cut(s) 266
CspCI CAANNNNNGTGG 2 cut(s) 592, 627
CviQI GTAC 9 cut(s) 119, 165, 431, 585, 645, 2252, 2912, 3005, 3095
DdeI CTNAG 9 cut(s) 114, 458, 807, 1044, 1379, 2058, 2110, 2318, 3043
DraI TTTAAA 1 cut(s) 2001
DraIII CACNNNGTG 1 cut(s) 908
EaeI YGGCCR 6 cut(s) 33, 47, 600, 2277, 2382, 2863
EagI CGGCCG 1 cut(s) 47
Eam1104I CTCTTC 5 cut(s) 189, 2034, 2265, 2460, 2975
EarI CTCTTC 5 cut(s) 189, 2034, 2265, 2460, 2975
EclXI CGGCCG 1 cut(s) 47
Eco130I CCWWGG 5 cut(s) 229, 288, 1491, 2122, 3032
Eco31I GGTCTC 1 cut(s) 746
Eco47I GGWCC 4 cut(s) 1648, 2020, 2133, 2798
Eco52I CGGCCG 1 cut(s) 47
Eco57I CTGAAG 4 cut(s) 1160, 1511, 2058, 2306
Eco81I CCTNAGG 1 cut(s) 114
Eco88I CYCGRG 2 cut(s) 1926, 3090
EcoNI CCTNNNNNAGG 1 cut(s) 2328
EcoO109I RGGNCCY 1 cut(s) 2314
EcoRII CCWGG 2 cut(s) 103, 1779
EcoT14I CCWWGG 5 cut(s) 229, 288, 1491, 2122, 3032
EcoT22I ATGCAT 1 cut(s) 711
ErhI CCWWGG 5 cut(s) 229, 288, 1491, 2122, 3032
Esp3I CGTCTC 1 cut(s) 54
FaqI GGGAC 7 cut(s) 86, 142, 1323, 1661, 2155, 2784, 2791
FbaI TGATCA 2 cut(s) 148, 2746
FblI GTMKAC 4 cut(s) 156, 1305, 2295, 2843
FokI GGATG 2 cut(s) 29, 90
FspBI CTAG 4 cut(s) 1335, 1683, 2183, 3002
GlaI GCGC 4 cut(s) 775, 1148, 2009, 2513
GsaI CCCAGC 2 cut(s) 725, 804
HaeII RGCGCY 1 cut(s) 2011
HaeIII GGCC 8 cut(s) 35, 49, 602, 1362, 2279, 2316, 2384, 2865
HapII CCGG 6 cut(s) 9, 46, 267, 507, 732, 1909
HgaI GACGC 1 cut(s) 79
HhaI GCGC 4 cut(s) 776, 1149, 2010, 2514
Hin6I GCGC 4 cut(s) 774, 1147, 2008, 2512
HinP1I GCGC 4 cut(s) 774, 1147, 2008, 2512
HpaII CCGG 6 cut(s) 9, 46, 267, 507, 732, 1909
HphI GGTGA 8 cut(s) 679, 920, 1421, 1439, 1673, 2218, 2358, 2984
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 9 cut(s) 464, 845, 923, 1487, 1553, 1927, 2033, 2584, 2674
HpyCH4IV ACGT 3 cut(s) 1522, 2254, 2497
HpyF3I CTNAG 9 cut(s) 114, 458, 807, 1044, 1379, 2058, 2110, 2318, 3043
HpySE526I ACGT 3 cut(s) 1522, 2254, 2497
HspAI GCGC 4 cut(s) 774, 1147, 2008, 2512
KpnI GGTACC 1 cut(s) 2915
Ksp22I TGATCA 2 cut(s) 148, 2746
LguI GCTCTTC 1 cut(s) 2460
LmnI GCTCC 2 cut(s) 1468, 2985
MaeI CTAG 4 cut(s) 1335, 1683, 2183, 3002
MaeII ACGT 3 cut(s) 1522, 2254, 2497
MaeIII GTNAC 5 cut(s) 91, 992, 1947, 2102, 2364
MbiI CCGCTC 1 cut(s) 52
MfeI CAATTG 2 cut(s) 1871, 2507
MflI RGATCY 7 cut(s) 445, 563, 793, 1117, 1196, 1258, 1756
MhlI GDGCHC 5 cut(s) 296, 904, 1084, 1186, 1473
MlsI TGGCCA 3 cut(s) 602, 2279, 2384
MluNI TGGCCA 3 cut(s) 602, 2279, 2384
MlyI GAGTC 3 cut(s) 974, 1547, 3033
MmeI TCCRAC 3 cut(s) 37, 1521, 2480
Mox20I TGGCCA 3 cut(s) 602, 2279, 2384
Mph1103I ATGCAT 1 cut(s) 711
MroXI GAANNNNTTC 3 cut(s) 855, 933, 2392
MscI TGGCCA 3 cut(s) 602, 2279, 2384
MslI CAYNNNNRTG 3 cut(s) 2127, 2232, 3063
Msp20I TGGCCA 3 cut(s) 602, 2279, 2384
MspA1I CMGCKG 1 cut(s) 2714
MspI CCGG 6 cut(s) 9, 46, 267, 507, 732, 1909
MspR9I CCNGG 4 cut(s) 10, 105, 507, 1781
MunI CAATTG 2 cut(s) 1871, 2507
MvaI CCWGG 2 cut(s) 105, 1781
NciI CCSGG 2 cut(s) 10, 507
NcoI CCATGG 3 cut(s) 288, 1491, 2122
NlaIV GGNNCC 8 cut(s) 447, 1649, 2134, 2504, 2576, 2800, 2913, 2987
NmeAIII GCCGAG 4 cut(s) 946, 1198, 1292, 2891
NmuCI GTSAC 3 cut(s) 91, 2102, 2364
NsiI ATGCAT 1 cut(s) 711
NspI RCATGY 5 cut(s) 401, 609, 680, 729, 2410
PaeR7I CTCGAG 1 cut(s) 1926
PagI TCATGA 4 cut(s) 339, 1276, 2188, 2227
PaqCI CACCTGC 1 cut(s) 1415
PasI CCCWGGG 1 cut(s) 104
PciI ACATGT 1 cut(s) 605
PciSI GCTCTTC 1 cut(s) 2460
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PdmI GAANNNNTTC 3 cut(s) 855, 933, 2392
PfeI GAWTC 7 cut(s) 415, 533, 1388, 1430, 1750, 2555, 2876
PflMI CCANNNNNTGG 3 cut(s) 1657, 2017, 2738
PinAI ACCGGT 1 cut(s) 266
PleI GAGTC 3 cut(s) 974, 1546, 3033
PpsI GAGTC 3 cut(s) 974, 1546, 3033
Ppu21I YACGTR 1 cut(s) 2255
PscI ACATGT 1 cut(s) 605
PshBI ATTAAT 1 cut(s) 1038
PsiI TTATAA 1 cut(s) 3077
Psp6I CCWGG 2 cut(s) 103, 1779
PspFI CCCAGC 2 cut(s) 721, 800
PspGI CCWGG 2 cut(s) 103, 1779
PspN4I GGNNCC 8 cut(s) 447, 1649, 2134, 2504, 2576, 2800, 2913, 2987
PspPI GGNCC 5 cut(s) 1648, 2020, 2133, 2314, 2798
PstI CTGCAG 3 cut(s) 75, 1947, 1995
PstNI CAGNNNCTG 3 cut(s) 830, 1705, 1753
PsuI RGATCY 7 cut(s) 445, 563, 793, 1117, 1196, 1258, 1756
PvuII CAGCTG 1 cut(s) 2714
RsaI GTAC 9 cut(s) 120, 166, 432, 586, 646, 2253, 2913, 3006, 3096
RsaNI GTAC 9 cut(s) 119, 165, 431, 585, 645, 2252, 2912, 3005, 3095
RseI CAYNNNNRTG 3 cut(s) 2127, 2232, 3063
SapI GCTCTTC 1 cut(s) 2460
Sau96I GGNCC 5 cut(s) 1648, 2020, 2133, 2314, 2798
ScaI AGTACT 1 cut(s) 166
SchI GAGTC 3 cut(s) 974, 1547, 3033
ScrFI CCNGG 4 cut(s) 10, 105, 507, 1781
SduI GDGCHC 5 cut(s) 296, 904, 1084, 1186, 1473
SfcI CTRYAG 5 cut(s) 71, 96, 636, 1943, 1991
Sfr274I CTCGAG 1 cut(s) 1926
SinI GGWCC 4 cut(s) 1648, 2020, 2133, 2798
SlaI CTCGAG 1 cut(s) 1926
SmiMI CAYNNNNRTG 3 cut(s) 2127, 2232, 3063
SmlI CTYRAG 7 cut(s) 333, 1220, 1326, 1580, 1739, 1926, 2399
SmoI CTYRAG 7 cut(s) 333, 1220, 1326, 1580, 1739, 1926, 2399
SsiI CCGC 4 cut(s) 50, 1828, 2647, 2709
SspMI CTAG 4 cut(s) 1335, 1683, 2183, 3002
StyD4I CCNGG 4 cut(s) 8, 103, 505, 1779
StyI CCWWGG 5 cut(s) 229, 288, 1491, 2122, 3032
TaiI ACGT 3 cut(s) 1525, 2257, 2500
TaqI TCGA 8 cut(s) 216, 529, 949, 1084, 1127, 1668, 1927, 2553
TatI WGTACW 4 cut(s) 164, 584, 644, 3004
TauI GCSGC 4 cut(s) 52, 1831, 2650, 2711
TfiI GAWTC 7 cut(s) 415, 533, 1388, 1430, 1750, 2555, 2876
TscAI CASTG 9 cut(s) 100, 301, 630, 904, 1919, 2775, 3002, 3067, 3108
TseFI GTSAC 3 cut(s) 91, 2102, 2364
Tsp45I GTSAC 3 cut(s) 91, 2102, 2364
TspGWI ACGGA 2 cut(s) 398, 1966
TspRI CASTG 9 cut(s) 100, 301, 630, 904, 1919, 2775, 3002, 3067, 3108
Van91I CCANNNNNTGG 3 cut(s) 1657, 2017, 2738
VneI GTGCAC 1 cut(s) 900
VpaK11BI GGWCC 4 cut(s) 1648, 2020, 2133, 2798
VspI ATTAAT 1 cut(s) 1038
XagI CCTNNNNNAGG 1 cut(s) 2328
XapI RAATTY 4 cut(s) 525, 1842, 2300, 2690
XceI RCATGY 5 cut(s) 401, 609, 680, 729, 2410
XhoI CTCGAG 1 cut(s) 1926
XmiI GTMKAC 4 cut(s) 156, 1305, 2295, 2843
XmnI GAANNNNTTC 3 cut(s) 855, 933, 2392
XspI CTAG 4 cut(s) 1335, 1683, 2183, 3002
ZrmI AGTACT 1 cut(s) 166
Zsp2I ATGCAT 1 cut(s) 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.