MD15G1077800.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005255
Physical Location & Seq
Forward (+)
5310881 .. 5318890
8010 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1077800.v1.1.491

Sequence Viewer

Length: 3120 bp
ATGGAAGCAGCAGGGGAGGACTGCTGCGTCAAAGTGGCGGTTCACATCCGGCCGCTCATCGGAGACGAGAAGCTTCAGGGGTGTAAAGATTGCGTCACTGTCGTCCCTGGGAAGCCTCAGGTACAAATTGGGACGCATTCGTTTACTTTTGATAATGTCTATGGGAGTACCGGTTCCCCCTCATCTGCAATGTTTGAAGAACGTATAGCTCCGCTGGTGGATGGTTTGTTTCACGGATATAATGCTACTGTCCTAGCGTATGGTCAGACGGGTTCTGGAAAAACGTATACCATGGGCACTGGTTTTAGAGATGGTTGCCAATCAGGAATTATCCCGCAAGTTATGAATGTATTGTTCGGCAAAATTGAAAATTTGAAGCATCAAACTGAATTCCAGTTGCATGTATCTTTTATTGAGATTCTAAAAGAAGAAGTACGAGACTTGCTCGATCCTAGTTTTTTGAGCAAACCAGAAGGTGCAAAAGAAGGTGCCAACGGGCATATGGTGAAAGTAGCATCCCCTGGAAAGCCACCAATACAAATTCGAGAATCATCAAATGGTGTTATTACACTGGCAGGGTCCACTGAACTCAGTGTAAGTACACTAAAAGAAATGGCTGCTTGCCTGGAACAAGGATCGTTGAGCAGAGCAACAGGGAGTACAAATATGAACAATCAATCAAGTCGCTCACATGCCATCTTCACCATTACATTAGAGCAAATGCATAAAGTCAACTCAGCATGCAATGAAAACAACGGTCTTAATGAGAGTATGAATGAAGAATATCTATGCGCCAAGTTGCATTTAGTAGATCTTGCTGGGTCTGAGCGAGCCAAGAGGACAGGTTCCGATGGCATGCGTTTTAAGGAAGGAGTTCATATTAATAAGGGTCTTCTTGCCCTTGGGAATGTTATCAGTGCACTTGGTGATGAGAAGAAGCGCAAAGAAGGTCTTCATGTGCCTTATCGAGATAGTAAACTTACTCGGCTTCTGCAGGATTCGCTTGGTGGTAACAGTCGAACTGTTATGATAGCCTGCATCAGTCCTGCTGATATCAATGCTGAAGAAACCCTGAACACTTTAAAGTATGCAAATCGTGCTCGCAATATCCAAAATAAGCCTATCGTAAATAGAGATCCCATGTCCAATGAGATGTTAAAGATGCGCCAACAGCTAGAGTTTTTGCAAGCTGAACTTTATTCACGTGGAGGAGGATCTTCTTCTGATGAAGTACAGGTTCTCAAGGAAAGAATTACTTGGCTTGAAGCTGCTAATGAGGATCTTTGTCGGGAACTTCATGAATATCGCAGTAGATGCAATGGTGTAAAGCCATGTGAAAGAGCTTCTCAAGATGATAGCCCCTGCTCGGTCAAAAGCGATGGTCTAAAAAGGGGTTTGCAAAGTTTAGAACCGGCTGACTATCAAATGGGCGATGCGATAACAGGTGATTCCCAGGAAATTGATGAAGAAGTAGCAAAAGAATGGGAGCACAACCTTCTGCAAAATACCATGGACAAAGAGTTGCATGAATTGAATAAACGTCTACAGCAAAAAGAGTCGGAGATGAAATTTTTTGAAGGGTCCGACACTCTGGCACTCAAGCAGCATTTTGGAAAGAAAATTTTGGAACTAGAAGATGAGAAAAGAACTGTGCAGCAAGAGAGGGACCGATTGCTGGGTGAAGTTGAAAATCTTTCTGCTGGTGATGGACAAACACAAAAGTTGCAAGATGTTCATAACCAGAAGTTAAAGGCACTTGAGGGACAGATTTCGGATCTTAAGAAGAAACAAGAAAGCCAGGTTCAACTTTTGAAGCAAAAACAAAAAAGTGATGAAGCAGCAAAGCGGCTGCAAGATGAAATCCAGTCTATAAAAGCACAAAAGGTTCAATTGCAACAAAGGATAAAACAAGAGGCAGAACAATTTCGGCAGTGGAAAGCCTCCCGAGAGAAGGAATTGCTGCAGTTACGGAAAGAAGGCAGGAGAAATGAATATGAAAGGCATAAGCTGCAAGCGTTAAATCAACGCCAGAAAATGGTTCTTCAAAGAAAGACTGAAGAGGCTGCAATGGCTACCAAGAGGCTGAAAGAATTGCTAGAAGCTCGTAAATCTTCTGCTCGTGACAACTCAGTTGTTGCCAATGGAAATGGGACACATGTACAGGGCAACGAGAAATCCTTACAACGGTGGCTTGATCATGAGCTTGAAGTTATGGTGAATGTGCATGAAGTTCGTCATGAATATGAGAAACAAAGTCAAGTACGAGCTGCACTGGCAGAAGAGTTGGCCATGCTGAAACAATTAAACGAATTTGCTTCGAAGGGCCTTAGTCCTCCAAGAGGAAAGAATGGCTTTGCCCGAGCATCCTCCATGTCACCTAATGCACGAATGGCCAGAATATCTTCACTTGAGAACATGCTTAGCATAACATCAAACTCAATAGTAGCAATGGCATCACAACTTTCAGAGGCAGAAGAACGGGAGCGTGCCTTTACCAACCGTGGACGTTGGACCCAATTGCGCTCAATGGCAGATGCAAAGAACTTGCTTCAATATATGTTCAATTCTCTTGCAGATACAAGGTGCCAGTTGTGGGAGAAAGAAATGGAAATCGATGAAATGAAAGAGCATCTCAAAGAACTCGTAGGTTTGTTGCGGCAGAGTGAGACACGAAGAAAGGAAGTTGAGAAGGAACTAAAAGTGAGAGAGCAAGCTGTTGCAGCTGCATTGGCAACATCTGCCTCGGCTGGCCATGATCAGGAGAACTCACAGAATCCACTGAAACACTTTGCTGATGACACGAATGGTCCCTTGTCCCCAATCTCCGTGCCAGCACAAAAACAGCTGAAATATACAGCAGGCATCGCCAGCGGCTCAGTCAGAGAATCGATAGCTTTCATAGATAAGACACGAAAGATGGTACCCATCGGGCATTTGCCAACAAAAAAACTAGCAGTTATAGGGCAAGCTGGGAAGCTATGGAGGTGGAAGAGGAGTCATCACCAGTGGTTAGTACAATTCAAATGGAAATGGCAGAAGCCTTGGAGACTCTCGGAATGGATTAGGCACAGCGATGAAACAATCATCAGGACCAAACCTCGCTTACAAGGTCAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000022 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000819 GO:0000910 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003676 GO:0003677 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005829 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005876 GO:0005881 GO:0005938 GO:0006355 GO:0006810 GO:0006890 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0008088 GO:0008089 GO:0008150 GO:0008574 GO:0009664 GO:0009832 GO:0009889 GO:0009891 GO:0009893 GO:0009937 GO:0009966 GO:0009987 GO:0010215 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010970 GO:0015630 GO:0016043 GO:0016192 GO:0016363 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019882 GO:0019884 GO:0019886 GO:0022402 GO:0022607 GO:0023051 GO:0030198 GO:0030496 GO:0030705 GO:0030863 GO:0030981 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032991 GO:0034399 GO:0042127 GO:0042546 GO:0042623 GO:0043062 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044085 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045171 GO:0045229 GO:0045893 GO:0045935 GO:0046907 GO:0048002 GO:0048193 GO:0048285 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051179 GO:0051225 GO:0051231 GO:0051234 GO:0051252 GO:0051254 GO:0051255 GO:0051256 GO:0051276 GO:0051301 GO:0051641 GO:0051649 GO:0055028 GO:0060255 GO:0061640 GO:0065007 GO:0070013 GO:0070726 GO:0070925 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071840 GO:0071944 GO:0080090 GO:0090307 GO:0097159 GO:0098813 GO:0098930 GO:0099080 GO:0099081 GO:0099111 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1901363 GO:1902680 GO:1902850 GO:1903047 GO:1903506 GO:1903508 GO:1990939 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kinesin

Protein Analysis

1040

Amino Acids

117.09

Weight (kDa)

8.74

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 17 - 370 3.6e-102 Kinesin motor domain
Microtub_bd PF16796 40 - 149 1e-18 Microtubule binding
KIF21A_4th PF25764 483 - 659 3.2e-55 KIF21A-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 26
Acc65I GGTACC 1 cut(s) 2920
AccB1I GGYRCC 3 cut(s) 488, 2583, 2920
AccB7I CCANNNNNTGG 1 cut(s) 2035
AccBSI CCGCTC 1 cut(s) 55
AccI GTMKAC 2 cut(s) 287, 1543
AciI CCGC 7 cut(s) 38, 53, 212, 335, 1846, 2656, 2871
AclWI GGATC 6 cut(s) 443, 643, 1130, 1222, 1287, 1782
AcoI YGGCCR 4 cut(s) 50, 2286, 2391, 2749
AcsI RAATTY 6 cut(s) 370, 389, 540, 1568, 1620, 2309
AcuI CTGAAG 3 cut(s) 59, 1083, 2076
AcvI CACGTG 1 cut(s) 1205
AdeI CACNNNGTG 1 cut(s) 926
AfiI CCNNNNNNNGG 9 cut(s) 59, 1366, 1675, 1951, 2035, 2184, 2339, 2593, 2758
AflII CTTAAG 1 cut(s) 1778
AflIII ACRYGT 1 cut(s) 2155
AgeI ACCGGT 1 cut(s) 170
AjnI CCWGG 5 cut(s) 106, 520, 624, 1452, 1797
AloI GAACNNNNNNTCC 2 cut(s) 157, 189
Alw21I GWGCWC 3 cut(s) 922, 1102, 1491
Alw26I GTCTC 4 cut(s) 57, 432, 2660, 3040
Alw44I GTGCAC 1 cut(s) 918
AlwI GGATC 6 cut(s) 443, 643, 1130, 1222, 1287, 1782
Ama87I CYCGRG 2 cut(s) 1944, 2358
AoxI GGCC 5 cut(s) 50, 2286, 2323, 2391, 2749
ApaLI GTGCAC 1 cut(s) 918
ApoI RAATTY 6 cut(s) 370, 389, 540, 1568, 1620, 2309
AseI ATTAAT 1 cut(s) 882
AsiGI ACCGGT 1 cut(s) 170
Asp700I GAANNNNTTC 4 cut(s) 873, 951, 1923, 2401
Asp718I GGTACC 1 cut(s) 2920
AspLEI GCGC 4 cut(s) 794, 942, 1167, 2523
AspS9I GGNCC 7 cut(s) 579, 1581, 1666, 2323, 2511, 2807, 3090
AsuHPI GGTGA 9 cut(s) 517, 694, 938, 1457, 1691, 1715, 2227, 2367, 2993
AsuII TTCGAA 1 cut(s) 2318
AvaI CYCGRG 2 cut(s) 1944, 2358
AvaII GGWCC 6 cut(s) 579, 1581, 1666, 2511, 2807, 3090
AxyI CCTNAGG 1 cut(s) 117
BaeGI GKGCMC 2 cut(s) 299, 922
BalI TGGCCA 3 cut(s) 2288, 2393, 2751
BanI GGYRCC 3 cut(s) 488, 2583, 2920
BauI CACGAG 1 cut(s) 2118
BbrPI CACGTG 1 cut(s) 1205
BbsI GAAGAC 2 cut(s) 884, 944
Bbv12I GWGCWC 3 cut(s) 922, 1102, 1491
BccI CCATC 8 cut(s) 215, 305, 704, 845, 1373, 1700, 2911, 2933
BcgI CGANNNNNNTGC 2 cut(s) 2213, 2247
BciT130I CCWGG 5 cut(s) 108, 522, 626, 1454, 1799
BclI TGATCA 2 cut(s) 2194, 2755
BcoDI GTCTC 4 cut(s) 57, 432, 2660, 3040
BfaI CTAG 6 cut(s) 254, 453, 1175, 1631, 2096, 2951
BfmI CTRYAG 3 cut(s) 992, 1544, 1961
BfrI CTTAAG 1 cut(s) 1778
BglII AGATCT 1 cut(s) 811
BlpI GCTNAGC 1 cut(s) 2420
Bme1390I CCNGG 5 cut(s) 108, 522, 626, 1454, 1799
Bme18I GGWCC 6 cut(s) 579, 1581, 1666, 2511, 2807, 3090
BmeT110I CYCGRG 2 cut(s) 1944, 2358
BmgT120I GGNCC 7 cut(s) 579, 1581, 1666, 2323, 2511, 2807, 3090
BmrFI CCNGG 5 cut(s) 108, 522, 626, 1454, 1799
BpiI GAAGAC 2 cut(s) 884, 944
BplI GAGNNNNNCTC 2 cut(s) 429, 461
Bpu1102I GCTNAGC 1 cut(s) 2420
Bpu14I TTCGAA 1 cut(s) 2318
BpuEI CTTGAG 5 cut(s) 1226, 1332, 1583, 1778, 2429
Bsa29I ATCGAT 2 cut(s) 2613, 2888
BsaAI YACGTR 1 cut(s) 1205
BsaWI WCCGGW 1 cut(s) 170
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 9 cut(s) 59, 1366, 1675, 1951, 2035, 2184, 2339, 2593, 2758
Bse118I RCCGGY 2 cut(s) 170, 1411
Bse1I ACTGG 7 cut(s) 304, 394, 576, 1864, 2277, 2587, 3004
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 5 cut(s) 195, 751, 1324, 2073, 2454
BseBI CCWGG 5 cut(s) 108, 522, 626, 1454, 1799
BseCI ATCGAT 2 cut(s) 2613, 2888
BseGI GGATG 4 cut(s) 45, 226, 515, 2363
BseLI CCNNNNNNNGG 9 cut(s) 59, 1366, 1675, 1951, 2035, 2184, 2339, 2593, 2758
BseMI GCAATG 5 cut(s) 195, 751, 1324, 2073, 2454
BseMII CTCAG 6 cut(s) 131, 604, 750, 816, 2142, 2889
BseNI ACTGG 7 cut(s) 304, 394, 576, 1864, 2277, 2587, 3004
BseRI GAGGAG 2 cut(s) 1224, 3007
BseSI GKGCMC 2 cut(s) 299, 922
BseX3I CGGCCG 1 cut(s) 50
BseYI CCCAGC 3 cut(s) 818, 1675, 2969
BsgI GTGCAG 2 cut(s) 1673, 2253
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 5 cut(s) 52, 2288, 2325, 2393, 2751
BshNI GGYRCC 3 cut(s) 488, 2583, 2920
BshTI ACCGGT 1 cut(s) 170
BshVI ATCGAT 2 cut(s) 2613, 2888
BsiEI CGRYCG 1 cut(s) 53
BsiHKAI GWGCWC 3 cut(s) 922, 1102, 1491
BsiHKCI CYCGRG 2 cut(s) 1944, 2358
BsiSI CCGG 3 cut(s) 49, 171, 1412
BslFI GGGAC 7 cut(s) 89, 145, 1679, 1776, 2164, 2793, 2800
BslI CCNNNNNNNGG 9 cut(s) 59, 1366, 1675, 1951, 2035, 2184, 2339, 2593, 2758
BsmAI GTCTC 4 cut(s) 57, 432, 2660, 3040
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 7 cut(s) 89, 145, 1679, 1776, 2164, 2793, 2800
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 5 cut(s) 52, 2288, 2325, 2393, 2751
BsoBI CYCGRG 2 cut(s) 1944, 2358
Bsp119I TTCGAA 1 cut(s) 2318
Bsp1286I GDGCHC 4 cut(s) 299, 922, 1102, 1491
Bsp1407I TGTACA 1 cut(s) 2158
Bsp143I GATC 9 cut(s) 448, 635, 811, 1135, 1214, 1279, 1774, 2194, 2755
Bsp1720I GCTNAGC 1 cut(s) 2420
Bsp19I CCATGG 2 cut(s) 291, 1509
BspACI CCGC 7 cut(s) 38, 53, 212, 335, 1846, 2656, 2871
BspANI GGCC 5 cut(s) 52, 2288, 2325, 2393, 2751
BspCNI CTCAG 6 cut(s) 130, 603, 749, 817, 2141, 2888
BspDI ATCGAT 2 cut(s) 2613, 2888
BspHI TCATGA 3 cut(s) 1297, 2197, 2236
BspMAI CTGCAG 2 cut(s) 996, 1965
BspPI GGATC 6 cut(s) 443, 643, 1130, 1222, 1287, 1782
BspT104I TTCGAA 1 cut(s) 2318
BspT107I GGYRCC 3 cut(s) 488, 2583, 2920
BspTI CTTAAG 1 cut(s) 1778
BsrBI CCGCTC 1 cut(s) 55
BsrDI GCAATG 5 cut(s) 195, 751, 1324, 2073, 2454
BsrFI RCCGGY 2 cut(s) 170, 1411
BsrGI TGTACA 1 cut(s) 2158
BsrI ACTGG 7 cut(s) 304, 394, 576, 1864, 2277, 2587, 3004
BssAI RCCGGY 2 cut(s) 170, 1411
BssMI GATC 9 cut(s) 448, 635, 811, 1135, 1214, 1279, 1774, 2194, 2755
BssNAI GTATAC 1 cut(s) 288
BssSI CACGAG 1 cut(s) 2118
BssT1I CCWWGG 4 cut(s) 291, 901, 1509, 3041
Bst1107I GTATAC 1 cut(s) 288
Bst2BI CACGAG 1 cut(s) 2118
Bst2UI CCWGG 5 cut(s) 108, 522, 626, 1454, 1799
Bst4CI ACNGT 8 cut(s) 100, 250, 758, 1016, 1024, 1651, 2187, 2501
Bst6I CTCTTC 3 cut(s) 2052, 2274, 2984
BstAFI CTTAAG 1 cut(s) 1778
BstAPI GCANNNNNTGC 4 cut(s) 1097, 1314, 2008, 2738
BstAUI TGTACA 1 cut(s) 2158
BstBAI YACGTR 1 cut(s) 1205
BstBI TTCGAA 1 cut(s) 2318
BstDEI CTNAG 8 cut(s) 117, 590, 736, 825, 2128, 2327, 2420, 2875
BstDSI CCRYGG 3 cut(s) 291, 1509, 2500
BstENI CCTNNNNNAGG 1 cut(s) 2337
BstF5I GGATG 4 cut(s) 45, 226, 515, 2363
BstHHI GCGC 4 cut(s) 794, 942, 1167, 2523
BstKTI GATC 9 cut(s) 451, 638, 814, 1138, 1217, 1282, 1777, 2197, 2758
BstMAI GTCTC 4 cut(s) 57, 432, 2660, 3040
BstMBI GATC 9 cut(s) 448, 635, 811, 1135, 1214, 1279, 1774, 2194, 2755
BstMCI CGRYCG 1 cut(s) 53
BstNI CCWGG 5 cut(s) 108, 522, 626, 1454, 1799
BstNSI RCATGY 6 cut(s) 404, 695, 744, 859, 2159, 2419
BstSCI CCNGG 5 cut(s) 106, 520, 624, 1452, 1797
BstSFI CTRYAG 3 cut(s) 992, 1544, 1961
BstSLI GKGCMC 2 cut(s) 299, 922
BstV2I GAAGAC 2 cut(s) 884, 944
BstX2I RGATCY 5 cut(s) 811, 1135, 1214, 1279, 1774
BstYI RGATCY 5 cut(s) 811, 1135, 1214, 1279, 1774
BstZ17I GTATAC 1 cut(s) 288
BstZI CGGCCG 1 cut(s) 50
Bsu15I ATCGAT 2 cut(s) 2613, 2888
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 5 cut(s) 52, 2288, 2325, 2393, 2751
BsuTUI ATCGAT 2 cut(s) 2613, 2888
BtgI CCRYGG 3 cut(s) 291, 1509, 2500
BtgZI GCGATG 4 cut(s) 1392, 1446, 2848, 3087
BtsCI GGATG 4 cut(s) 45, 226, 515, 2363
BtsI GCAGTG 1 cut(s) 1937
CciI TCATGA 3 cut(s) 1297, 2197, 2236
CfoI GCGC 4 cut(s) 794, 942, 1167, 2523
Cfr10I RCCGGY 2 cut(s) 170, 1411
Cfr13I GGNCC 7 cut(s) 579, 1581, 1666, 2323, 2511, 2807, 3090
ClaI ATCGAT 2 cut(s) 2613, 2888
CseI GACGC 3 cut(s) 16, 82, 142
CspAI ACCGGT 1 cut(s) 170
DdeI CTNAG 8 cut(s) 117, 590, 736, 825, 2128, 2327, 2420, 2875
DpnI GATC 9 cut(s) 450, 637, 813, 1137, 1216, 1281, 1776, 2196, 2757
DpnII GATC 9 cut(s) 448, 635, 811, 1135, 1214, 1279, 1774, 2194, 2755
DraI TTTAAA 1 cut(s) 1083
DraIII CACNNNGTG 1 cut(s) 926
DrdI GACNNNNNNGTC 1 cut(s) 26
DseDI GACNNNNNNGTC 1 cut(s) 26
EaeI YGGCCR 4 cut(s) 50, 2286, 2391, 2749
EagI CGGCCG 1 cut(s) 50
Eam1104I CTCTTC 3 cut(s) 2052, 2274, 2984
EarI CTCTTC 3 cut(s) 2052, 2274, 2984
EclXI CGGCCG 1 cut(s) 50
Eco130I CCWWGG 4 cut(s) 291, 901, 1509, 3041
Eco32I GATATC 1 cut(s) 1054
Eco47I GGWCC 6 cut(s) 579, 1581, 1666, 2511, 2807, 3090
Eco52I CGGCCG 1 cut(s) 50
Eco57I CTGAAG 3 cut(s) 59, 1083, 2076
Eco72I CACGTG 1 cut(s) 1205
Eco81I CCTNAGG 1 cut(s) 117
Eco88I CYCGRG 2 cut(s) 1944, 2358
EcoNI CCTNNNNNAGG 1 cut(s) 2337
EcoO109I RGGNCCY 1 cut(s) 2323
EcoRI GAATTC 1 cut(s) 389
EcoRII CCWGG 5 cut(s) 106, 520, 624, 1452, 1797
EcoRV GATATC 1 cut(s) 1054
EcoT14I CCWWGG 4 cut(s) 291, 901, 1509, 3041
EcoT22I ATGCAT 1 cut(s) 726
ErhI CCWWGG 4 cut(s) 291, 901, 1509, 3041
Esp3I CGTCTC 1 cut(s) 57
FalI AAGNNNNNCTT 8 cut(s) 936, 968, 1179, 1211, 1240, 1272, 2336, 2368
FaqI GGGAC 7 cut(s) 89, 145, 1679, 1776, 2164, 2793, 2800
FauI CCCGC 1 cut(s) 342
FauNDI CATATG 1 cut(s) 501
FbaI TGATCA 2 cut(s) 2194, 2755
FblI GTMKAC 2 cut(s) 287, 1543
FokI GGATG 4 cut(s) 32, 233, 502, 2350
FspBI CTAG 6 cut(s) 254, 453, 1175, 1631, 2096, 2951
GlaI GCGC 4 cut(s) 793, 941, 1166, 2522
GsaI CCCAGC 3 cut(s) 822, 1679, 2973
HaeIII GGCC 5 cut(s) 52, 2288, 2325, 2393, 2751
HapII CCGG 3 cut(s) 49, 171, 1412
HgaI GACGC 3 cut(s) 16, 82, 142
HhaI GCGC 4 cut(s) 794, 942, 1167, 2523
Hin6I GCGC 4 cut(s) 792, 940, 1165, 2521
HinP1I GCGC 4 cut(s) 792, 940, 1165, 2521
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 9 cut(s) 418, 548, 998, 1448, 1556, 2773, 2885, 2995, 3048
HpaII CCGG 3 cut(s) 49, 171, 1412
HphI GGTGA 9 cut(s) 517, 694, 938, 1457, 1691, 1715, 2227, 2367, 2993
HpyCH4III ACNGT 8 cut(s) 100, 250, 758, 1016, 1024, 1651, 2187, 2501
HpyCH4IV ACGT 5 cut(s) 202, 284, 1204, 1540, 2506
HpyF3I CTNAG 8 cut(s) 117, 590, 736, 825, 2128, 2327, 2420, 2875
HpySE526I ACGT 5 cut(s) 202, 284, 1204, 1540, 2506
HspAI GCGC 4 cut(s) 792, 940, 1165, 2521
KpnI GGTACC 1 cut(s) 2924
Ksp22I TGATCA 2 cut(s) 2194, 2755
Kzo9I GATC 9 cut(s) 448, 635, 811, 1135, 1214, 1279, 1774, 2194, 2755
LmnI GCTCC 3 cut(s) 214, 1486, 2482
MaeI CTAG 6 cut(s) 254, 453, 1175, 1631, 2096, 2951
MaeII ACGT 5 cut(s) 202, 284, 1204, 1540, 2506
MaeIII GTNAC 5 cut(s) 94, 1010, 1965, 2120, 2373
MalI GATC 9 cut(s) 450, 637, 813, 1137, 1216, 1281, 1776, 2196, 2757
MbiI CCGCTC 1 cut(s) 55
MboI GATC 9 cut(s) 448, 635, 811, 1135, 1214, 1279, 1774, 2194, 2755
MfeI CAATTG 2 cut(s) 1889, 2516
MflI RGATCY 5 cut(s) 811, 1135, 1214, 1279, 1774
MhlI GDGCHC 4 cut(s) 299, 922, 1102, 1491
MlsI TGGCCA 3 cut(s) 2288, 2393, 2751
MluNI TGGCCA 3 cut(s) 2288, 2393, 2751
MlyI GAGTC 3 cut(s) 1565, 3004, 3042
MmeI TCCRAC 3 cut(s) 1539, 1608, 2489
Mox20I TGGCCA 3 cut(s) 2288, 2393, 2751
Mph1103I ATGCAT 1 cut(s) 726
MroXI GAANNNNTTC 4 cut(s) 873, 951, 1923, 2401
MscI TGGCCA 3 cut(s) 2288, 2393, 2751
MseI TTAA 9 cut(s) 762, 864, 882, 1082, 1157, 1748, 1779, 2018, 2303
MslI CAYNNNNRTG 2 cut(s) 2241, 3072
Msp20I TGGCCA 3 cut(s) 2288, 2393, 2751
MspA1I CMGCKG 4 cut(s) 214, 2723, 2845, 2871
MspCI CTTAAG 1 cut(s) 1778
MspI CCGG 3 cut(s) 49, 171, 1412
MspR9I CCNGG 5 cut(s) 108, 522, 626, 1454, 1799
MunI CAATTG 2 cut(s) 1889, 2516
Mva1269I GAATGC 1 cut(s) 136
MvaI CCWGG 5 cut(s) 108, 522, 626, 1454, 1799
NcoI CCATGG 2 cut(s) 291, 1509
NdeI CATATG 1 cut(s) 501
NdeII GATC 9 cut(s) 448, 635, 811, 1135, 1214, 1279, 1774, 2194, 2755
NmeAIII GCCGAG 2 cut(s) 964, 2723
NmuCI GTSAC 3 cut(s) 94, 2120, 2373
NsiI ATGCAT 1 cut(s) 726
NspI RCATGY 6 cut(s) 404, 695, 744, 859, 2159, 2419
NspV TTCGAA 1 cut(s) 2318
PaeI GCATGC 2 cut(s) 744, 859
PagI TCATGA 3 cut(s) 1297, 2197, 2236
PasI CCCWGGG 1 cut(s) 107
PciI ACATGT 1 cut(s) 2155
PctI GAATGC 1 cut(s) 136
PdmI GAANNNNTTC 4 cut(s) 873, 951, 1923, 2401
PfeI GAWTC 6 cut(s) 418, 548, 998, 1448, 2773, 2885
PflMI CCANNNNNTGG 1 cut(s) 2035
PinAI ACCGGT 1 cut(s) 170
PleI GAGTC 3 cut(s) 1564, 3003, 3042
PmaCI CACGTG 1 cut(s) 1205
PmlI CACGTG 1 cut(s) 1205
PpsI GAGTC 3 cut(s) 1564, 3003, 3042
Ppu21I YACGTR 1 cut(s) 1205
PscI ACATGT 1 cut(s) 2155
PshBI ATTAAT 1 cut(s) 882
Psp6I CCWGG 5 cut(s) 106, 520, 624, 1452, 1797
PspCI CACGTG 1 cut(s) 1205
PspFI CCCAGC 3 cut(s) 818, 1675, 2969
PspGI CCWGG 5 cut(s) 106, 520, 624, 1452, 1797
PspPI GGNCC 7 cut(s) 579, 1581, 1666, 2323, 2511, 2807, 3090
PstI CTGCAG 2 cut(s) 996, 1965
PsuI RGATCY 5 cut(s) 811, 1135, 1214, 1279, 1774
PvuII CAGCTG 2 cut(s) 2723, 2845
RseI CAYNNNNRTG 2 cut(s) 2241, 3072
SaqAI TTAA 9 cut(s) 762, 864, 882, 1082, 1157, 1748, 1779, 2018, 2303
Sau3AI GATC 9 cut(s) 448, 635, 811, 1135, 1214, 1279, 1774, 2194, 2755
Sau96I GGNCC 7 cut(s) 579, 1581, 1666, 2323, 2511, 2807, 3090
SchI GAGTC 3 cut(s) 1565, 3004, 3042
ScrFI CCNGG 5 cut(s) 108, 522, 626, 1454, 1799
SduI GDGCHC 4 cut(s) 299, 922, 1102, 1491
SfcI CTRYAG 3 cut(s) 992, 1544, 1961
SfuI TTCGAA 1 cut(s) 2318
SinI GGWCC 6 cut(s) 579, 1581, 1666, 2511, 2807, 3090
SmiMI CAYNNNNRTG 2 cut(s) 2241, 3072
SmlI CTYRAG 6 cut(s) 1241, 1347, 1598, 1757, 1778, 2408
SmoI CTYRAG 6 cut(s) 1241, 1347, 1598, 1757, 1778, 2408
SphI GCATGC 2 cut(s) 744, 859
SsiI CCGC 7 cut(s) 38, 53, 212, 335, 1846, 2656, 2871
SspMI CTAG 6 cut(s) 254, 453, 1175, 1631, 2096, 2951
StyD4I CCNGG 5 cut(s) 106, 520, 624, 1452, 1797
StyI CCWWGG 4 cut(s) 291, 901, 1509, 3041
TaaI ACNGT 8 cut(s) 100, 250, 758, 1016, 1024, 1651, 2187, 2501
TaiI ACGT 5 cut(s) 205, 287, 1207, 1543, 2509
TaqI TCGA 7 cut(s) 447, 544, 967, 1018, 2318, 2613, 2888
TaqII GACCGA 2 cut(s) 1357, 1683
TatI WGTACW 5 cut(s) 599, 659, 1231, 2158, 3013
TauI GCSGC 4 cut(s) 55, 1849, 2659, 2874
TfiI GAWTC 6 cut(s) 418, 548, 998, 1448, 2773, 2885
Tru1I TTAA 9 cut(s) 762, 864, 882, 1082, 1157, 1748, 1779, 2018, 2303
Tru9I TTAA 9 cut(s) 762, 864, 882, 1082, 1157, 1748, 1779, 2018, 2303
TseFI GTSAC 3 cut(s) 94, 2120, 2373
Tsp45I GTSAC 3 cut(s) 94, 2120, 2373
TspGWI ACGGA 3 cut(s) 249, 1984, 2815
Van91I CCANNNNNTGG 1 cut(s) 2035
Vha464I CTTAAG 1 cut(s) 1778
VneI GTGCAC 1 cut(s) 918
VpaK11BI GGWCC 6 cut(s) 579, 1581, 1666, 2511, 2807, 3090
VspI ATTAAT 1 cut(s) 882
XagI CCTNNNNNAGG 1 cut(s) 2337
XapI RAATTY 6 cut(s) 370, 389, 540, 1568, 1620, 2309
XceI RCATGY 6 cut(s) 404, 695, 744, 859, 2159, 2419
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XmiI GTMKAC 2 cut(s) 287, 1543
XmnI GAANNNNTTC 4 cut(s) 873, 951, 1923, 2401
XspI CTAG 6 cut(s) 254, 453, 1175, 1631, 2096, 2951
Zsp2I ATGCAT 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.