MD08G1102500.v1.1

Phosphatidate phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0020389
Physical Location & Seq
Reverse (-)
8691255 .. 8692545
1291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1102500.v1.1.491

Sequence Viewer

Length: 342 bp
ATGTTTTCCTCAGTTATCAGGAGGGCTCCTCATGAATTCAAAATCGCATGCTTGGAGGATATCAAATCATTGTTCCCTTCTGATTGCAATCCATTCTATGCTGGTTTTGGAAACAGAGATACCGATGAGTTCAGCTACCTTAAGGTTGGAATCCCGAAAGGAAAAATATTCATTATTAATCCCAAGGGTGAGGTTGTTGTGAACCGCCGTGTTGACACAAAGTCATATACCTCCCTTCATGCTCTTGTGAACGGCATGTTTCCACCTACAAACTCATCTGAGCAGGAGGACTTTAATACGTGGAATTTCTGGAAACTCCCACCGCGCGATATTGATGTTTGA

Protein Analysis

114

Amino Acids

12.96

Weight (kDa)

6.72

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LNS2 PF08235 4 - 88 3.9e-31 LNS2 (Lipin/Ned1/Smp2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020389)

Species Orthologous Gene IDs
malus_domestica MD08G1102500.v1.1
rosa_chinensis RchiOBHm_Chr5g0048511
rosa_multiflora Rmu_co8061612.1_g000001
rosa_roxburghii Rroxscaffold_1G00033290
rosa_wichuraiana Rw5G029820 Rw5G029950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 325, 327
AciI CCGC 2 cut(s) 205, 323
AcsI RAATTY 2 cut(s) 35, 304
AflII CTTAAG 1 cut(s) 140
AgsI TTSAA 1 cut(s) 40
AhdI GACNNNNNGTC 1 cut(s) 220
AluBI AGCT 1 cut(s) 135
AluI AGCT 1 cut(s) 135
ApoI RAATTY 2 cut(s) 35, 304
AseI ATTAAT 1 cut(s) 177
AspLEI GCGC 1 cut(s) 327
AsuHPI GGTGA 1 cut(s) 200
BanII GRGCYC 1 cut(s) 28
BceAI ACGGC 2 cut(s) 192, 268
BfrI CTTAAG 1 cut(s) 140
BmeRI GACNNNNNGTC 1 cut(s) 220
BmiI GGNNCC 1 cut(s) 27
BplI GAGNNNNNCTC 2 cut(s) 13, 45
BsaAI YACGTR 1 cut(s) 300
BsaBI GATNNNNATC 1 cut(s) 87
BsaJI CCNNGG 1 cut(s) 183
Bse8I GATNNNNATC 1 cut(s) 87
BseDI CCNNGG 1 cut(s) 183
BseJI GATNNNNATC 1 cut(s) 87
BseMII CTCAG 2 cut(s) 24, 270
BseRI GAGGAG 1 cut(s) 18
Bsh1236I CGCG 2 cut(s) 325, 327
Bsp1286I GDGCHC 1 cut(s) 28
BspACI CCGC 2 cut(s) 205, 323
BspCNI CTCAG 2 cut(s) 23, 271
BspFNI CGCG 2 cut(s) 325, 327
BspHI TCATGA 1 cut(s) 31
BspLI GGNNCC 1 cut(s) 27
BspTI CTTAAG 1 cut(s) 140
BssECI CCNNGG 1 cut(s) 183
BssT1I CCWWGG 1 cut(s) 183
BstAFI CTTAAG 1 cut(s) 140
BstBAI YACGTR 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 49
BstDEI CTNAG 2 cut(s) 10, 279
BstFNI CGCG 2 cut(s) 325, 327
BstHHI GCGC 1 cut(s) 327
BstNSI RCATGY 2 cut(s) 51, 259
BstUI CGCG 2 cut(s) 325, 327
Cac8I GCNNGC 1 cut(s) 49
CciI TCATGA 1 cut(s) 31
CfoI GCGC 1 cut(s) 327
CviAII CATG 4 cut(s) 32, 48, 239, 256
CviJI RGCY 2 cut(s) 26, 135
CviKI_1 RGCY 2 cut(s) 26, 135
DdeI CTNAG 2 cut(s) 10, 279
DriI GACNNNNNGTC 1 cut(s) 220
Eam1105I GACNNNNNGTC 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 28
Eco32I GATATC 1 cut(s) 61
EcoRI GAATTC 1 cut(s) 35
EcoRV GATATC 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 183
EcoT38I GRGCYC 1 cut(s) 28
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 4 cut(s) 35, 51, 242, 259
FaiI YATR 7 cut(s) 33, 49, 99, 226, 228, 240, 257
FatI CATG 4 cut(s) 31, 47, 238, 255
FriOI GRGCYC 1 cut(s) 28
GlaI GCGC 1 cut(s) 326
HhaI GCGC 1 cut(s) 327
Hin1II CATG 4 cut(s) 35, 51, 242, 259
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 1 cut(s) 150
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 3 cut(s) 202, 214, 250
Hpy188I TCNGA 2 cut(s) 82, 280
Hpy188III TCNNGA 4 cut(s) 19, 32, 154, 310
Hpy8I GTNNAC 3 cut(s) 202, 214, 250
HpyAV CCTTC 2 cut(s) 87, 245
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 1 cut(s) 87
HpyF3I CTNAG 2 cut(s) 10, 279
HpySE526I ACGT 1 cut(s) 299
Hsp92II CATG 4 cut(s) 35, 51, 242, 259
HspAI GCGC 1 cut(s) 325
LmnI GCTCC 1 cut(s) 31
LpnPI CCDG 4 cut(s) 4, 87, 269, 295
MaeII ACGT 1 cut(s) 299
MhlI GDGCHC 1 cut(s) 28
MluCI AATT 2 cut(s) 35, 304
MmeI TCCRAC 1 cut(s) 127
MnlI CCTC 7 cut(s) 15, 19, 39, 49, 184, 241, 280
MseI TTAA 3 cut(s) 141, 177, 294
MspCI CTTAAG 1 cut(s) 140
MvnI CGCG 2 cut(s) 325, 327
NlaIII CATG 4 cut(s) 35, 51, 242, 259
NlaIV GGNNCC 1 cut(s) 27
NspI RCATGY 2 cut(s) 51, 259
PaeI GCATGC 1 cut(s) 51
PagI TCATGA 1 cut(s) 31
PfeI GAWTC 1 cut(s) 150
Ppu21I YACGTR 1 cut(s) 300
PshBI ATTAAT 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 27
SaqAI TTAA 3 cut(s) 141, 177, 294
SduI GDGCHC 1 cut(s) 28
SetI ASST 7 cut(s) 137, 141, 147, 195, 233, 268, 302
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
SphI GCATGC 1 cut(s) 51
Sse9I AATT 2 cut(s) 35, 304
SsiI CCGC 2 cut(s) 205, 323
SspI AATATT 1 cut(s) 168
StyI CCWWGG 1 cut(s) 183
TaiI ACGT 1 cut(s) 302
TasI AATT 2 cut(s) 35, 304
TfiI GAWTC 1 cut(s) 150
Tru1I TTAA 3 cut(s) 141, 177, 294
Tru9I TTAA 3 cut(s) 141, 177, 294
TspDTI ATGAA 3 cut(s) 48, 160, 227
Vha464I CTTAAG 1 cut(s) 140
VspI ATTAAT 1 cut(s) 177
XapI RAATTY 2 cut(s) 35, 304
XceI RCATGY 2 cut(s) 51, 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.