MD08G1115500.v1.1

Casein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016276
Physical Location & Seq
Reverse (-)
10459405 .. 10464482
5078 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1115500.v1.1.491

Sequence Viewer

Length: 1314 bp
ATGGAGAGGGTCGTCGCAGAGAAGTACAAATTAGGTCGCAAAATTGGGAGCGGCTCGTTCGGTGAATTGTATCTTGCAACGCATATAGAGACTGGCGAGAATTTGGCGGCCAAGATTGAAAATAACAAGACAAAACATCCGCAACTTTTGTACGAGGCAAAGCTTTATAAAATTCTGCAGGGAGGAAGTGGTATTGCAAGCATAAGATGGGCGGGATTTGATAAGGACGATAATGTTCTCGTCATCGACTTGCTGGGACCGAGTCTTGAAGACCTCTTTGTGTACTGTGGGAGGAGGTTTTCACTGAAGACAGTCTTAATGTTGGCAGATCAGATGATTACCAGAATAGAATATGTACATTCTAAAGGGTTTCTTCATAGAGACATCAAACCAGACAACTTCCTCATGGGTCTTGGCAGGAAAGCAAATCAAGTTTACGTTATTGATTTTGGACTTGCAAAAAGATTTCGGGACCAAAATACACACCGGCATATTCCGTACAGAGAGAACAGAAGCTTAACAGGAACTGCACGCTATGCTAGTTGCAATACCCATTTGGGAATAGATCAGAGTCGCCGGGATGATTTGGAGTCACTTGGATATGTTCTTCTGTACTTCTTAAGAGGAAGCCTTCCTTGGCAGGGTCTAAAAGCTGCAACAAAGAAGCAAAAATATGATAAGATTTGTGAGAAGAAGTTATCAACTCCCATTGAGGCCCTATGTAAGTCACATCCTGTGGAGTTTGCCTCGTACTTCCATTATTGCCACTCTTTAACATTTGATCAACGCCCGGATTATGCATTCTTGAAGCGCTTGTTTCGTGACTTGTTTAGCCGTGAAGGATACGAGTTTGACTATATATTTGACTGGACAATTCTAAAATATCAGCAGGCACAGCAGACAAGGATTCAGGGTCAATCATCTAAACCGCAGCCACCTGTAGTAGTCCACGGTCGAGAAGTTCCTAGTGGTCAAGCAGTACCAATGAGGCTTAAAGGTAAAGGCGATGTTTCTAGATCAGCTGAGCTCACAGAACGAGAGCGGCCAAGCAATCATGCGCATCCAGATGTTCATATGCAATATAGATCCTCAACAACCCAGAATTCGGGTGCTAATAATCCTGTTGAGAAACATAATGTGAGCAATGCACCTATGCCACCTACTGTATTTACTCTCAGAAGACATTTTCAAAAACCAGAGGGGCCGGCTGAAAATGCAAACTTAGGCCGTAGAGTTGGGAATCAAAATGGTGCTTCAAGCAGTTGGATGCCGTCAGTAAACCAAATTTCTTCAGCAAACTACAGATCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04310 ko04340 ko04341 ko04390 ko04391 ko04392 ko04540 ko04710 ko04711 map04068 map04310 map04340 map04341 map04390 map04391 map04392 map04540 map04710 map04711
Pfam Domains
Protein Families
Pkinase

Protein Analysis

438

Amino Acids

49.85

Weight (kDa)

9.51

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 9 - 227 3.8e-27 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 10 - 269 4.6e-13 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 110 - 206 2.3e-08 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016276)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g35730 FvH4_2g35730 FvH4_2g35730
malus_domestica MD08G1115500.v1.1 MD15G1094700.v1.1
prunus_persica Prupe.1G449900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306051
rosa_laevigata RLG00000010828
rosa_roxburghii Rroxscaffold_7G00162090
rosa_rugosa Rorug06G0346600 Rorug06G0346700
rosa_samantha Rh6BG442100 Rh6CG473200 Rh6DG459500
rosa_wichuraiana Rw6G039990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 168
Acc16I TGCGCA 1 cut(s) 1059
AccBSI CCGCTC 2 cut(s) 51, 1042
AciI CCGC 6 cut(s) 51, 107, 140, 212, 929, 1042
AclWI GGATC 1 cut(s) 1080
AcoI YGGCCR 2 cut(s) 108, 1043
AcsI RAATTY 4 cut(s) 100, 171, 1102, 1284
AcuI CTGAAG 2 cut(s) 326, 1275
AfaI GTAC 8 cut(s) 26, 152, 284, 357, 500, 614, 752, 981
AfeI AGCGCT 1 cut(s) 812
AfiI CCNNNNNNNGG 2 cut(s) 641, 1105
AflII CTTAAG 1 cut(s) 619
AgsI TTSAA 5 cut(s) 119, 269, 808, 1190, 1257
AjuI GAANNNNNNNTTGG 2 cut(s) 619, 651
AluBI AGCT 5 cut(s) 163, 516, 653, 1022, 1027
AluI AGCT 5 cut(s) 163, 516, 653, 1022, 1027
Alw21I GWGCWC 1 cut(s) 1029
Alw26I GTCTC 2 cut(s) 83, 375
AlwI GGATC 1 cut(s) 1080
AlwNI CAGNNNCTG 1 cut(s) 527
Aor51HI AGCGCT 1 cut(s) 812
AoxI GGCC 5 cut(s) 108, 714, 1043, 1202, 1225
ApeKI GCWGC 2 cut(s) 653, 931
ApoI RAATTY 4 cut(s) 100, 171, 1102, 1284
ArsI GACNNNNNNTTYG 2 cut(s) 845, 877
AspLEI GCGC 2 cut(s) 813, 1060
AspS9I GGNCC 4 cut(s) 257, 472, 715, 1202
AsuC2I CCSGG 2 cut(s) 578, 791
AsuHPI GGTGA 1 cut(s) 74
AvaII GGWCC 2 cut(s) 257, 472
BanII GRGCYC 1 cut(s) 1029
BbsI GAAGAC 3 cut(s) 276, 314, 1186
Bbv12I GWGCWC 1 cut(s) 1029
BbvI GCAGC 2 cut(s) 640, 943
BccI CCATC 1 cut(s) 201
BceAI ACGGC 3 cut(s) 819, 1212, 1255
BciVI GTATCC 1 cut(s) 836
BclI TGATCA 1 cut(s) 781
BcnI CCSGG 2 cut(s) 578, 791
BcoDI GTCTC 2 cut(s) 83, 375
BfaI CTAG 3 cut(s) 540, 966, 1014
BfmI CTRYAG 3 cut(s) 176, 939, 1300
BfoI RGCGCY 1 cut(s) 814
BfrI CTTAAG 1 cut(s) 619
BfuI GTATCC 1 cut(s) 836
BglII AGATCT 1 cut(s) 1304
BisI GCNGC 5 cut(s) 52, 108, 654, 932, 1043
BlpI GCTNAGC 1 cut(s) 1023
BlsI GCNGC 5 cut(s) 53, 109, 655, 933, 1044
Bme1390I CCNGG 2 cut(s) 578, 791
Bme18I GGWCC 2 cut(s) 257, 472
BmgT120I GGNCC 4 cut(s) 257, 472, 715, 1202
BmiI GGNNCC 3 cut(s) 258, 473, 1203
BmrFI CCNGG 2 cut(s) 578, 791
BmsI GCATC 2 cut(s) 1069, 1257
BpiI GAAGAC 3 cut(s) 276, 314, 1186
BplI GAGNNNNNCTC 2 cut(s) 731, 763
Bpu1102I GCTNAGC 1 cut(s) 1023
BpuMI CCSGG 2 cut(s) 578, 791
BsaJI CCNNGG 2 cut(s) 635, 949
Bsc4I CCNNNNNNNGG 2 cut(s) 641, 1105
Bse118I RCCGGY 2 cut(s) 486, 1204
Bse1I ACTGG 2 cut(s) 97, 872
Bse3DI GCAATG 1 cut(s) 1150
BseDI CCNNGG 2 cut(s) 635, 949
BseGI GGATG 5 cut(s) 136, 586, 730, 1060, 1272
BseLI CCNNNNNNNGG 2 cut(s) 641, 1105
BseMI GCAATG 1 cut(s) 1150
BseMII CTCAG 2 cut(s) 1014, 1189
BseNI ACTGG 2 cut(s) 97, 872
BseRI GAGGAG 1 cut(s) 307
BseXI GCAGC 2 cut(s) 640, 943
BseYI CCCAGC 1 cut(s) 253
BsgI GTGCAG 1 cut(s) 513
Bsh1285I CGRYCG 1 cut(s) 955
BshFI GGCC 5 cut(s) 110, 716, 1045, 1204, 1227
BsiEI CGRYCG 1 cut(s) 955
BsiHKAI GWGCWC 1 cut(s) 1029
BsiSI CCGG 4 cut(s) 487, 577, 791, 1205
BslFI GGGAC 2 cut(s) 270, 485
BslI CCNNNNNNNGG 2 cut(s) 641, 1105
BsmAI GTCTC 2 cut(s) 83, 375
BsmFI GGGAC 2 cut(s) 270, 485
BsmI GAATGC 1 cut(s) 800
BsnI GGCC 5 cut(s) 110, 716, 1045, 1204, 1227
Bsp1286I GDGCHC 1 cut(s) 1029
Bsp1407I TGTACA 1 cut(s) 355
Bsp143I GATC 6 cut(s) 328, 565, 781, 1016, 1085, 1304
Bsp1720I GCTNAGC 1 cut(s) 1023
BspACI CCGC 6 cut(s) 51, 107, 140, 212, 929, 1042
BspANI GGCC 5 cut(s) 110, 716, 1045, 1204, 1227
BspCNI CTCAG 2 cut(s) 1015, 1188
BspLI GGNNCC 3 cut(s) 258, 473, 1203
BspMAI CTGCAG 1 cut(s) 180
BspPI GGATC 1 cut(s) 1080
BspTI CTTAAG 1 cut(s) 619
BsrBI CCGCTC 2 cut(s) 51, 1042
BsrDI GCAATG 1 cut(s) 1150
BsrFI RCCGGY 2 cut(s) 486, 1204
BsrGI TGTACA 1 cut(s) 355
BsrI ACTGG 2 cut(s) 97, 872
BssAI RCCGGY 2 cut(s) 486, 1204
BssECI CCNNGG 2 cut(s) 635, 949
BssMI GATC 6 cut(s) 328, 565, 781, 1016, 1085, 1304
BssT1I CCWWGG 1 cut(s) 635
Bst4CI ACNGT 4 cut(s) 287, 313, 953, 1165
BstAFI CTTAAG 1 cut(s) 619
BstAPI GCANNNNNTGC 1 cut(s) 536
BstAUI TGTACA 1 cut(s) 355
BstC8I GCNNGC 4 cut(s) 199, 532, 891, 1206
BstDEI CTNAG 3 cut(s) 1023, 1175, 1222
BstDSI CCRYGG 1 cut(s) 949
BstF5I GGATG 5 cut(s) 136, 586, 730, 1060, 1272
BstH2I RGCGCY 1 cut(s) 814
BstHHI GCGC 2 cut(s) 813, 1060
BstKTI GATC 6 cut(s) 331, 568, 784, 1019, 1088, 1307
BstMAI GTCTC 2 cut(s) 83, 375
BstMBI GATC 6 cut(s) 328, 565, 781, 1016, 1085, 1304
BstMCI CGRYCG 1 cut(s) 955
BstMWI GCNNNNNNNGC 3 cut(s) 536, 895, 1214
BstSCI CCNGG 2 cut(s) 576, 789
BstSFI CTRYAG 3 cut(s) 176, 939, 1300
BstV1I GCAGC 2 cut(s) 640, 943
BstV2I GAAGAC 3 cut(s) 276, 314, 1186
BstX2I RGATCY 2 cut(s) 1085, 1304
BstYI RGATCY 2 cut(s) 1085, 1304
BsuI GTATCC 1 cut(s) 836
BsuRI GGCC 5 cut(s) 110, 716, 1045, 1204, 1227
BtgI CCRYGG 1 cut(s) 949
BtgZI GCGATG 1 cut(s) 1020
BtsCI GGATG 5 cut(s) 136, 586, 730, 1060, 1272
BtsIMutI CAGTG 1 cut(s) 302
Cac8I GCNNGC 4 cut(s) 199, 532, 891, 1206
CaiI CAGNNNCTG 1 cut(s) 527
CfoI GCGC 2 cut(s) 813, 1060
Cfr10I RCCGGY 2 cut(s) 486, 1204
Cfr13I GGNCC 4 cut(s) 257, 472, 715, 1202
Csp6I GTAC 8 cut(s) 25, 151, 283, 356, 499, 613, 751, 980
CviAII CATG 2 cut(s) 406, 1055
CviQI GTAC 8 cut(s) 25, 151, 283, 356, 499, 613, 751, 980
DdeI CTNAG 3 cut(s) 1023, 1175, 1222
DpnI GATC 6 cut(s) 330, 567, 783, 1018, 1087, 1306
DpnII GATC 6 cut(s) 328, 565, 781, 1016, 1085, 1304
EaeI YGGCCR 2 cut(s) 108, 1043
Ecl136II GAGCTC 1 cut(s) 1027
Eco130I CCWWGG 1 cut(s) 635
Eco24I GRGCYC 1 cut(s) 1029
Eco47I GGWCC 2 cut(s) 257, 472
Eco47III AGCGCT 1 cut(s) 812
Eco53kI GAGCTC 1 cut(s) 1027
Eco57I CTGAAG 2 cut(s) 326, 1275
EcoICRI GAGCTC 1 cut(s) 1027
EcoO109I RGGNCCY 1 cut(s) 715
EcoRI GAATTC 1 cut(s) 1102
EcoT14I CCWWGG 1 cut(s) 635
EcoT22I ATGCAT 1 cut(s) 802
EcoT38I GRGCYC 1 cut(s) 1029
ErhI CCWWGG 1 cut(s) 635
FaeI CATG 2 cut(s) 409, 1058
FalI AAGNNNNNCTT 6 cut(s) 299, 331, 357, 389, 619, 651
FaqI GGGAC 2 cut(s) 270, 485
FatI CATG 2 cut(s) 405, 1054
FauI CCCGC 1 cut(s) 205
FauNDI CATATG 1 cut(s) 1074
FbaI TGATCA 1 cut(s) 781
Fnu4HI GCNGC 5 cut(s) 52, 108, 654, 932, 1043
FokI GGATG 5 cut(s) 123, 593, 717, 1047, 1279
FriOI GRGCYC 1 cut(s) 1029
Fsp4HI GCNGC 5 cut(s) 52, 108, 654, 932, 1043
FspAI RTGCGCAY 1 cut(s) 1059
FspBI CTAG 3 cut(s) 540, 966, 1014
FspI TGCGCA 1 cut(s) 1059
GlaI GCGC 2 cut(s) 812, 1059
GluI GCNGC 5 cut(s) 52, 108, 654, 932, 1043
GsaI CCCAGC 1 cut(s) 257
HaeII RGCGCY 1 cut(s) 814
HaeIII GGCC 5 cut(s) 110, 716, 1045, 1204, 1227
HapII CCGG 4 cut(s) 487, 577, 791, 1205
HhaI GCGC 2 cut(s) 813, 1060
Hin1II CATG 2 cut(s) 409, 1058
Hin6I GCGC 2 cut(s) 811, 1058
HinP1I GCGC 2 cut(s) 811, 1058
HindIII AAGCTT 2 cut(s) 161, 514
HinfI GANTC 5 cut(s) 262, 571, 590, 907, 1240
HpaII CCGG 4 cut(s) 487, 577, 791, 1205
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 4 cut(s) 283, 436, 949, 1279
Hpy188I TCNGA 4 cut(s) 333, 570, 1178, 1309
Hpy188III TCNNGA 7 cut(s) 266, 470, 805, 821, 956, 1014, 1064
Hpy8I GTNNAC 4 cut(s) 283, 436, 949, 1279
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 2 cut(s) 641, 833
HpyCH4III ACNGT 4 cut(s) 287, 313, 953, 1165
HpyCH4IV ACGT 1 cut(s) 438
HpyF10VI GCNNNNNNNGC 3 cut(s) 536, 895, 1214
HpyF3I CTNAG 3 cut(s) 1023, 1175, 1222
HpySE526I ACGT 1 cut(s) 438
Hsp92II CATG 2 cut(s) 409, 1058
HspAI GCGC 2 cut(s) 811, 1058
KroI GCCGGC 1 cut(s) 1204
KroNI GCCGGC 1 cut(s) 1206
Ksp22I TGATCA 1 cut(s) 781
Kzo9I GATC 6 cut(s) 328, 565, 781, 1016, 1085, 1304
LmnI GCTCC 1 cut(s) 48
Lsp1109I GCAGC 2 cut(s) 640, 943
LweI GCATC 2 cut(s) 1069, 1257
MaeI CTAG 3 cut(s) 540, 966, 1014
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 3 cut(s) 591, 726, 821
MalI GATC 6 cut(s) 330, 567, 783, 1018, 1087, 1306
MbiI CCGCTC 2 cut(s) 51, 1042
MboI GATC 6 cut(s) 328, 565, 781, 1016, 1085, 1304
MboII GAAGA 7 cut(s) 281, 319, 365, 599, 703, 1191, 1281
MflI RGATCY 2 cut(s) 1085, 1304
MhlI GDGCHC 1 cut(s) 1029
MluCI AATT 8 cut(s) 29, 42, 65, 100, 171, 873, 1102, 1284
MlyI GAGTC 3 cut(s) 271, 580, 599
MmeI TCCRAC 1 cut(s) 1244
Mph1103I ATGCAT 1 cut(s) 802
MroNI GCCGGC 1 cut(s) 1204
MseI TTAA 5 cut(s) 317, 518, 620, 773, 993
MslI CAYNNNNRTG 1 cut(s) 1065
MspA1I CMGCKG 1 cut(s) 1022
MspCI CTTAAG 1 cut(s) 619
MspI CCGG 4 cut(s) 487, 577, 791, 1205
MspR9I CCNGG 2 cut(s) 578, 791
Mva1269I GAATGC 1 cut(s) 800
MwoI GCNNNNNNNGC 3 cut(s) 536, 895, 1214
NaeI GCCGGC 1 cut(s) 1206
NciI CCSGG 2 cut(s) 578, 791
NdeI CATATG 1 cut(s) 1074
NdeII GATC 6 cut(s) 328, 565, 781, 1016, 1085, 1304
NgoMIV GCCGGC 1 cut(s) 1204
NlaIII CATG 2 cut(s) 409, 1058
NlaIV GGNNCC 3 cut(s) 258, 473, 1203
NmuCI GTSAC 3 cut(s) 591, 726, 821
NsbI TGCGCA 1 cut(s) 1059
NsiI ATGCAT 1 cut(s) 802
PctI GAATGC 1 cut(s) 800
PdiI GCCGGC 1 cut(s) 1206
PfeI GAWTC 2 cut(s) 907, 1240
PflFI GACNNNGTC 1 cut(s) 261
PkrI GCNGC 5 cut(s) 53, 109, 655, 933, 1044
PleI GAGTC 3 cut(s) 270, 579, 598
PpsI GAGTC 3 cut(s) 270, 579, 598
PsiI TTATAA 1 cut(s) 168
Psp124BI GAGCTC 1 cut(s) 1029
PspFI CCCAGC 1 cut(s) 253
PspN4I GGNNCC 3 cut(s) 258, 473, 1203
PspPI GGNCC 4 cut(s) 257, 472, 715, 1202
PstI CTGCAG 1 cut(s) 180
PstNI CAGNNNCTG 1 cut(s) 527
PsuI RGATCY 2 cut(s) 1085, 1304
PsyI GACNNNGTC 1 cut(s) 261
PvuII CAGCTG 1 cut(s) 1022
RsaI GTAC 8 cut(s) 26, 152, 284, 357, 500, 614, 752, 981
RsaNI GTAC 8 cut(s) 25, 151, 283, 356, 499, 613, 751, 980
RseI CAYNNNNRTG 1 cut(s) 1065
SacI GAGCTC 1 cut(s) 1029
SaqAI TTAA 5 cut(s) 317, 518, 620, 773, 993
SatI GCNGC 5 cut(s) 52, 108, 654, 932, 1043
Sau3AI GATC 6 cut(s) 328, 565, 781, 1016, 1085, 1304
Sau96I GGNCC 4 cut(s) 257, 472, 715, 1202
SchI GAGTC 3 cut(s) 271, 580, 599
ScrFI CCNGG 2 cut(s) 578, 791
SduI GDGCHC 1 cut(s) 1029
SfaNI GCATC 2 cut(s) 1069, 1257
SfcI CTRYAG 3 cut(s) 176, 939, 1300
SinI GGWCC 2 cut(s) 257, 472
SmiMI CAYNNNNRTG 1 cut(s) 1065
SmlI CTYRAG 1 cut(s) 619
SmoI CTYRAG 1 cut(s) 619
Sse9I AATT 8 cut(s) 29, 42, 65, 100, 171, 873, 1102, 1284
SsiI CCGC 6 cut(s) 51, 107, 140, 212, 929, 1042
SspMI CTAG 3 cut(s) 540, 966, 1014
SstI GAGCTC 1 cut(s) 1029
StyD4I CCNGG 2 cut(s) 576, 789
StyI CCWWGG 1 cut(s) 635
TaaI ACNGT 4 cut(s) 287, 313, 953, 1165
TaiI ACGT 1 cut(s) 441
TaqI TCGA 2 cut(s) 246, 955
TaqII GACCGA 1 cut(s) 274
TasI AATT 8 cut(s) 29, 42, 65, 100, 171, 873, 1102, 1284
TatI WGTACW 4 cut(s) 24, 282, 355, 612
TauI GCSGC 3 cut(s) 54, 110, 1045
TfiI GAWTC 2 cut(s) 907, 1240
Tru1I TTAA 5 cut(s) 317, 518, 620, 773, 993
Tru9I TTAA 5 cut(s) 317, 518, 620, 773, 993
TscAI CASTG 1 cut(s) 309
TseFI GTSAC 3 cut(s) 591, 726, 821
TseI GCWGC 2 cut(s) 653, 931
Tsp45I GTSAC 3 cut(s) 591, 726, 821
TspDTI ATGAA 2 cut(s) 365, 1061
TspGWI ACGGA 1 cut(s) 486
TspRI CASTG 1 cut(s) 309
Tth111I GACNNNGTC 1 cut(s) 261
Vha464I CTTAAG 1 cut(s) 619
VpaK11BI GGWCC 2 cut(s) 257, 472
XapI RAATTY 4 cut(s) 100, 171, 1102, 1284
XbaI TCTAGA 1 cut(s) 1013
XspI CTAG 3 cut(s) 540, 966, 1014
Zsp2I ATGCAT 1 cut(s) 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.