Rh6CG473200

Casein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0016276
Physical Location & Seq
Forward (+)
64272581 .. 64276782
4202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG473200.1

Sequence Viewer

Length: 1179 bp
ATGGAATTTCTTGTTAGAATTAGGCTCTCGACGATTTTGGAAAGTAACAAGACGAAGCATTTACAGCTTATGTATGAGGCCAAGCTGTATAAAGTTCTACAGGGAGGAAGCGGCATTGCAAGCATGAAGTGGGCGGGGTTTGATCATGATGATAACGTTGTCATCATAGACTTGTTGGGGCCGAGTCTTGAAGACTTATTTGTATACTGTGGGAGGAAGTTTTCACTGAAAACAGTCTTAATGCTGGCCGAACAGATGATTACGAGAATAGAGTATGTGCATTCGAAAGGGTTTTTGCATAGAGACATCAAACCAGACAATTATCTCATGGGTCTTGGCAGGAAAGCAAATCAGGTTTACATCATTGACTTTGGACTTGCGAAAAGATATCGGGACCCAACTACAAATCGGCACATTCCATACAGAGAAAACAAAAGCTTAACAGGAACTGCACGATATGCTAGTTGCAATACCCATTTGGGAATCGATCAGAGTCGTCGGGATGATTTGGAGTCACTTGGCTATGTTCTTCTGTACTTCTTAAGAGGAAGCCTTCCTTGGCAGGGTCTAAAAGCTGCAACGAAGAAGCAAAAATATGATAAGATTTGTGAGAAGAAGTTATCAACTCCCATTGAGGTGCTATGTAAGGCGCATCCTGTGGAGTTTGCATCCTACTTCCACTATTGCCATTCCTTAACATTTGATCAACGTCCGGATTACTCATTCTTGAAGCGTCTGTTTCGTGACTTATTCACCCGCGAAGGATTTGAGTTTGATTATGTATTTGACTGGACAATCCTAAAGTACCAGCAGGCGCAACAGACAAGGGCTCATGGTCAATCAGCTCCAGCTGTAGCGACAAATGGTCAAGTAGTGACTAGCAGTCGAGCAGTGCCTATGGATGTGAGGAATCAGGGAGTTGATGATGTTTCTAGACAAGCTCAGCCTCGAGAACATAAAAGATCAAGTCATCTTGTTCATATTCACAGGCCATTTAGATCATCTGCAGTCCAGAATTTGAGTGCTAATAACTCTATTGAAAAACATAATACGGGCAATGCTCTCAAAAGAAATTTACCAAAACCTGAGGGGCCAGCTGAAATTGCTAATGTAGGTCGCCAGCACGGTGCTTCAAGCAGTTGGATGCCATCTCCAAATCGAACTTCTCAAGCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04310 ko04340 ko04341 ko04390 ko04391 ko04392 ko04540 ko04710 ko04711 map04068 map04310 map04340 map04341 map04390 map04391 map04392 map04540 map04710 map04711
Pfam Domains
Protein Families
Pkinase

Protein Analysis

392

Amino Acids

44.85

Weight (kDa)

9.62

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 16 - 201 5.7e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 17 - 243 5.9e-09 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 86 - 180 4.5e-08 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016276)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g35730 FvH4_2g35730 FvH4_2g35730
malus_domestica MD08G1115500.v1.1 MD15G1094700.v1.1
prunus_persica Prupe.1G449900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306051
rosa_laevigata RLG00000010828
rosa_roxburghii Rroxscaffold_7G00162090
rosa_rugosa Rorug06G0346600 Rorug06G0346700
rosa_samantha Rh6BG442100 Rh6CG473200 Rh6DG459500
rosa_wichuraiana Rw6G039990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 204
AccII CGCG 1 cut(s) 759
AccIII TCCGGA 1 cut(s) 712
AciI CCGC 3 cut(s) 111, 134, 757
AclI AACGTT 1 cut(s) 156
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 3 cut(s) 5, 1015, 1072
AfaI GTAC 2 cut(s) 536, 806
AfiI CCNNNNNNNGG 1 cut(s) 563
AflII CTTAAG 1 cut(s) 541
AgsI TTSAA 4 cut(s) 191, 730, 1040, 1134
AhdI GACNNNNNGTC 2 cut(s) 864, 882
AjuI GAANNNNNNNTTGG 4 cut(s) 541, 573, 1147, 1179
AluBI AGCT 8 cut(s) 67, 85, 438, 575, 845, 851, 941, 1097
AluI AGCT 8 cut(s) 67, 85, 438, 575, 845, 851, 941, 1097
Alw26I GTCTC 1 cut(s) 297
AlwNI CAGNNNCTG 1 cut(s) 449
Ama87I CYCGRG 1 cut(s) 948
Aor13HI TCCGGA 1 cut(s) 712
AoxI GGCC 5 cut(s) 78, 179, 246, 989, 1091
ApeKI GCWGC 1 cut(s) 575
ApoI RAATTY 3 cut(s) 5, 1015, 1072
AspLEI GCGC 2 cut(s) 652, 817
AspS9I GGNCC 3 cut(s) 179, 394, 1091
AsuHPI GGTGA 1 cut(s) 745
AsuII TTCGAA 1 cut(s) 284
AvaI CYCGRG 1 cut(s) 948
AvaII GGWCC 1 cut(s) 394
AxyI CCTNAGG 1 cut(s) 1086
BanII GRGCYC 1 cut(s) 832
BbsI GAAGAC 1 cut(s) 198
BbvI GCAGC 1 cut(s) 562
BccI CCATC 1 cut(s) 1156
BclI TGATCA 2 cut(s) 142, 703
BcoDI GTCTC 1 cut(s) 297
BfaI CTAG 3 cut(s) 462, 879, 933
BfmI CTRYAG 3 cut(s) 98, 852, 1005
BfrI CTTAAG 1 cut(s) 541
BisI GCNGC 2 cut(s) 112, 576
BlpI GCTNAGC 1 cut(s) 942
BlsI GCNGC 2 cut(s) 113, 577
Bme18I GGWCC 1 cut(s) 394
BmeRI GACNNNNNGTC 2 cut(s) 864, 882
BmeT110I CYCGRG 1 cut(s) 948
BmgT120I GGNCC 3 cut(s) 179, 394, 1091
BmiI GGNNCC 4 cut(s) 180, 395, 396, 1092
BmsI GCATC 3 cut(s) 661, 677, 1134
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 831
Bpu1102I GCTNAGC 1 cut(s) 942
Bpu14I TTCGAA 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 1152
Bsa29I ATCGAT 1 cut(s) 486
BsaJI CCNNGG 1 cut(s) 557
BsaWI WCCGGW 1 cut(s) 712
Bsc4I CCNNNNNNNGG 1 cut(s) 563
Bse1I ACTGG 1 cut(s) 794
Bse21I CCTNAGG 1 cut(s) 1086
Bse3DI GCAATG 2 cut(s) 114, 1063
BseAI TCCGGA 1 cut(s) 712
BseCI ATCGAT 1 cut(s) 486
BseDI CCNNGG 1 cut(s) 557
BseGI GGATG 5 cut(s) 508, 652, 668, 907, 1149
BseLI CCNNNNNNNGG 1 cut(s) 563
BseMI GCAATG 2 cut(s) 114, 1063
BseMII CTCAG 2 cut(s) 956, 1077
BseNI ACTGG 1 cut(s) 794
BseXI GCAGC 1 cut(s) 562
BsgI GTGCAG 1 cut(s) 435
Bsh1236I CGCG 1 cut(s) 759
BshFI GGCC 5 cut(s) 80, 181, 248, 991, 1093
BshVI ATCGAT 1 cut(s) 486
BsiHKCI CYCGRG 1 cut(s) 948
BsiSI CCGG 1 cut(s) 713
BslFI GGGAC 1 cut(s) 407
BslI CCNNNNNNNGG 1 cut(s) 563
BsmAI GTCTC 1 cut(s) 297
BsmFI GGGAC 1 cut(s) 407
BsmI GAATGC 1 cut(s) 280
BsnI GGCC 5 cut(s) 80, 181, 248, 991, 1093
BsoBI CYCGRG 1 cut(s) 948
Bsp119I TTCGAA 1 cut(s) 284
Bsp1286I GDGCHC 1 cut(s) 832
Bsp13I TCCGGA 1 cut(s) 712
Bsp143I GATC 5 cut(s) 142, 487, 703, 962, 998
Bsp1720I GCTNAGC 1 cut(s) 942
BspACI CCGC 3 cut(s) 111, 134, 757
BspANI GGCC 5 cut(s) 80, 181, 248, 991, 1093
BspCNI CTCAG 2 cut(s) 955, 1078
BspDI ATCGAT 1 cut(s) 486
BspEI TCCGGA 1 cut(s) 712
BspFNI CGCG 1 cut(s) 759
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 4 cut(s) 180, 395, 396, 1092
BspMAI CTGCAG 1 cut(s) 1009
BspT104I TTCGAA 1 cut(s) 284
BspTI CTTAAG 1 cut(s) 541
BsrDI GCAATG 2 cut(s) 114, 1063
BsrI ACTGG 1 cut(s) 794
BssECI CCNNGG 1 cut(s) 557
BssMI GATC 5 cut(s) 142, 487, 703, 962, 998
BssNAI GTATAC 1 cut(s) 205
BssT1I CCWWGG 1 cut(s) 557
Bst1107I GTATAC 1 cut(s) 205
Bst4CI ACNGT 3 cut(s) 209, 235, 1127
BstAFI CTTAAG 1 cut(s) 541
BstAPI GCANNNNNTGC 1 cut(s) 458
BstBI TTCGAA 1 cut(s) 284
BstC8I GCNNGC 5 cut(s) 121, 246, 813, 1095, 1121
BstDEI CTNAG 2 cut(s) 942, 1086
BstF5I GGATG 5 cut(s) 508, 652, 668, 907, 1149
BstFNI CGCG 1 cut(s) 759
BstHHI GCGC 2 cut(s) 652, 817
BstKTI GATC 5 cut(s) 145, 490, 706, 965, 1001
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 5 cut(s) 142, 487, 703, 962, 998
BstMWI GCNNNNNNNGC 4 cut(s) 64, 120, 458, 1103
BstSFI CTRYAG 3 cut(s) 98, 852, 1005
BstUI CGCG 1 cut(s) 759
BstV1I GCAGC 1 cut(s) 562
BstV2I GAAGAC 1 cut(s) 198
BstZ17I GTATAC 1 cut(s) 205
Bsu15I ATCGAT 1 cut(s) 486
Bsu36I CCTNAGG 1 cut(s) 1086
BsuRI GGCC 5 cut(s) 80, 181, 248, 991, 1093
BsuTUI ATCGAT 1 cut(s) 486
BtsCI GGATG 5 cut(s) 508, 652, 668, 907, 1149
BtsI GCAGTG 1 cut(s) 897
BtsIMutI CAGTG 2 cut(s) 224, 897
Cac8I GCNNGC 5 cut(s) 121, 246, 813, 1095, 1121
CaiI CAGNNNCTG 1 cut(s) 449
CciI TCATGA 1 cut(s) 145
CfoI GCGC 2 cut(s) 652, 817
Cfr13I GGNCC 3 cut(s) 179, 394, 1091
ClaI ATCGAT 1 cut(s) 486
CseI GACGC 1 cut(s) 722
Csp6I GTAC 2 cut(s) 535, 805
CviAII CATG 4 cut(s) 124, 146, 328, 833
CviQI GTAC 2 cut(s) 535, 805
DdeI CTNAG 2 cut(s) 942, 1086
DpnI GATC 5 cut(s) 144, 489, 705, 964, 1000
DpnII GATC 5 cut(s) 142, 487, 703, 962, 998
DriI GACNNNNNGTC 2 cut(s) 864, 882
EaeI YGGCCR 1 cut(s) 246
Eam1105I GACNNNNNGTC 2 cut(s) 864, 882
Eco130I CCWWGG 1 cut(s) 557
Eco24I GRGCYC 1 cut(s) 832
Eco32I GATATC 1 cut(s) 389
Eco47I GGWCC 1 cut(s) 394
Eco81I CCTNAGG 1 cut(s) 1086
Eco88I CYCGRG 1 cut(s) 948
EcoO109I RGGNCCY 1 cut(s) 394
EcoRV GATATC 1 cut(s) 389
EcoT14I CCWWGG 1 cut(s) 557
EcoT38I GRGCYC 1 cut(s) 832
ErhI CCWWGG 1 cut(s) 557
FaeI CATG 4 cut(s) 127, 149, 331, 836
FalI AAGNNNNNCTT 2 cut(s) 541, 573
FaqI GGGAC 1 cut(s) 407
FatI CATG 4 cut(s) 123, 145, 327, 832
FauI CCCGC 2 cut(s) 127, 764
FbaI TGATCA 2 cut(s) 142, 703
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 112, 576
FokI GGATG 5 cut(s) 515, 639, 655, 914, 1156
FriOI GRGCYC 1 cut(s) 832
Fsp4HI GCNGC 2 cut(s) 112, 576
FspBI CTAG 3 cut(s) 462, 879, 933
GlaI GCGC 2 cut(s) 651, 816
GluI GCNGC 2 cut(s) 112, 576
GsuI CTGGAG 1 cut(s) 831
HaeIII GGCC 5 cut(s) 80, 181, 248, 991, 1093
HapII CCGG 1 cut(s) 713
HgaI GACGC 1 cut(s) 722
HhaI GCGC 2 cut(s) 652, 817
Hin1II CATG 4 cut(s) 127, 149, 331, 836
Hin6I GCGC 2 cut(s) 650, 815
HinP1I GCGC 2 cut(s) 650, 815
HindIII AAGCTT 1 cut(s) 436
HinfI GANTC 5 cut(s) 184, 483, 493, 512, 910
HpaII CCGG 1 cut(s) 713
HphI GGTGA 1 cut(s) 745
Hpy166II GTNNAC 2 cut(s) 205, 358
Hpy188I TCNGA 1 cut(s) 492
Hpy8I GTNNAC 2 cut(s) 205, 358
Hpy99I CGWCG 2 cut(s) 34, 501
HpyAV CCTTC 2 cut(s) 563, 755
HpyCH4III ACNGT 3 cut(s) 209, 235, 1127
HpyCH4IV ACGT 2 cut(s) 156, 709
HpyCH4V TGCA 8 cut(s) 119, 280, 298, 452, 468, 578, 668, 1007
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 120, 458, 1103
HpyF3I CTNAG 2 cut(s) 942, 1086
HpySE526I ACGT 2 cut(s) 156, 709
Hsp92II CATG 4 cut(s) 127, 149, 331, 836
HspAI GCGC 2 cut(s) 650, 815
KflI GGGWCCC 1 cut(s) 394
Kpn2I TCCGGA 1 cut(s) 712
Ksp22I TGATCA 2 cut(s) 142, 703
Kzo9I GATC 5 cut(s) 142, 487, 703, 962, 998
LmnI GCTCC 1 cut(s) 850
Lsp1109I GCAGC 1 cut(s) 562
LweI GCATC 3 cut(s) 661, 677, 1134
MaeI CTAG 3 cut(s) 462, 879, 933
MaeII ACGT 2 cut(s) 156, 709
MaeIII GTNAC 4 cut(s) 44, 513, 743, 874
MalI GATC 5 cut(s) 144, 489, 705, 964, 1000
MboI GATC 5 cut(s) 142, 487, 703, 962, 998
MboII GAAGA 4 cut(s) 203, 521, 595, 625
MhlI GDGCHC 1 cut(s) 832
MluCI AATT 6 cut(s) 5, 18, 319, 1015, 1072, 1101
MlyI GAGTC 3 cut(s) 193, 502, 521
MmeI TCCRAC 1 cut(s) 1121
MnlI CCTC 8 cut(s) 70, 98, 207, 539, 628, 900, 957, 1081
MroI TCCGGA 1 cut(s) 712
MseI TTAA 4 cut(s) 239, 440, 542, 695
MslI CAYNNNNRTG 1 cut(s) 635
MspA1I CMGCKG 2 cut(s) 851, 1097
MspCI CTTAAG 1 cut(s) 541
MspI CCGG 1 cut(s) 713
Mva1269I GAATGC 1 cut(s) 280
MvnI CGCG 1 cut(s) 759
MwoI GCNNNNNNNGC 4 cut(s) 64, 120, 458, 1103
NdeII GATC 5 cut(s) 142, 487, 703, 962, 998
NlaIII CATG 4 cut(s) 127, 149, 331, 836
NlaIV GGNNCC 4 cut(s) 180, 395, 396, 1092
NmeAIII GCCGAG 1 cut(s) 207
NmuCI GTSAC 3 cut(s) 513, 743, 874
NspV TTCGAA 1 cut(s) 284
PaeR7I CTCGAG 1 cut(s) 948
PagI TCATGA 1 cut(s) 145
PctI GAATGC 1 cut(s) 280
PfeI GAWTC 2 cut(s) 483, 910
PkrI GCNGC 2 cut(s) 113, 577
PleI GAGTC 3 cut(s) 192, 501, 520
PpsI GAGTC 3 cut(s) 192, 501, 520
PpuMI RGGWCCY 1 cut(s) 394
Psp1406I AACGTT 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 394
PspN4I GGNNCC 4 cut(s) 180, 395, 396, 1092
PspPI GGNCC 3 cut(s) 179, 394, 1091
PspPPI RGGWCCY 1 cut(s) 394
PstI CTGCAG 1 cut(s) 1009
PstNI CAGNNNCTG 1 cut(s) 449
PvuII CAGCTG 2 cut(s) 851, 1097
RsaI GTAC 2 cut(s) 536, 806
RsaNI GTAC 2 cut(s) 535, 805
RseI CAYNNNNRTG 1 cut(s) 635
SaqAI TTAA 4 cut(s) 239, 440, 542, 695
SatI GCNGC 2 cut(s) 112, 576
Sau3AI GATC 5 cut(s) 142, 487, 703, 962, 998
Sau96I GGNCC 3 cut(s) 179, 394, 1091
SchI GAGTC 3 cut(s) 193, 502, 521
SduI GDGCHC 1 cut(s) 832
SfaNI GCATC 3 cut(s) 661, 677, 1134
SfcI CTRYAG 3 cut(s) 98, 852, 1005
Sfr274I CTCGAG 1 cut(s) 948
SfuI TTCGAA 1 cut(s) 284
SinI GGWCC 1 cut(s) 394
SlaI CTCGAG 1 cut(s) 948
SmiMI CAYNNNNRTG 1 cut(s) 635
SmlI CTYRAG 3 cut(s) 541, 948, 1167
SmoI CTYRAG 3 cut(s) 541, 948, 1167
Sse9I AATT 6 cut(s) 5, 18, 319, 1015, 1072, 1101
SsiI CCGC 3 cut(s) 111, 134, 757
SspMI CTAG 3 cut(s) 462, 879, 933
StyI CCWWGG 1 cut(s) 557
TaaI ACNGT 3 cut(s) 209, 235, 1127
TaiI ACGT 2 cut(s) 159, 712
TaqI TCGA 6 cut(s) 29, 284, 486, 886, 949, 1159
TasI AATT 6 cut(s) 5, 18, 319, 1015, 1072, 1101
TatI WGTACW 1 cut(s) 534
TauI GCSGC 1 cut(s) 114
TfiI GAWTC 2 cut(s) 483, 910
Tru1I TTAA 4 cut(s) 239, 440, 542, 695
Tru9I TTAA 4 cut(s) 239, 440, 542, 695
TscAI CASTG 2 cut(s) 231, 897
TseFI GTSAC 3 cut(s) 513, 743, 874
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 3 cut(s) 513, 743, 874
TspDTI ATGAA 2 cut(s) 140, 968
TspRI CASTG 2 cut(s) 231, 897
Vha464I CTTAAG 1 cut(s) 541
VpaK11BI GGWCC 1 cut(s) 394
XapI RAATTY 3 cut(s) 5, 1015, 1072
XbaI TCTAGA 1 cut(s) 932
XhoI CTCGAG 1 cut(s) 948
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 3 cut(s) 462, 879, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.