MD15G1094700.v1.1

Casein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016276
Physical Location & Seq
Reverse (-)
6633572 .. 6638441
4870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1094700.v1.1.491

Sequence Viewer

Length: 1503 bp
ATGGAGAGGATCATCGCAGAGAAGTACAAATTAGGTCGCAAAATTGGGAGCGGATCGTTCGGCGAATTGTATCTTGCAACCCATATAGAGACTGGCGAGAATTTGGCGGCGAAGATTGAAAATAACAAGACAAAACATCCACAACTTTTGTACGAGGCAAAGCTTTATAAAATTCTACAGGGAGGAAGTGGTATTGCAAGCATAAGATGGGCGGGATTTGATAGGGACGATAATGTCCTCGTCATTGACTTGCTGGGACCGAGTCTTGAAGACCTCTTTGTGTACTGTGGGAGGAGGTTTTCACTGAAGACAGTCTTAATGTTGGCTGATCAGATGATTACAAGAATAGAATATGTACATTCTAAAGGGTTTTTGCATAGAGACATCAAACCAGACAACTTCCTCATGGGTCTTGGCAGGAAAGCAAATCAGGTTTATGTTATTGATTTTGGACTTGCAAAAAGATTTCGGGACCAAAATACACACCGGCATATTCCGTACAGAGAAAACAAAAGCTTAACAGGAACTGCACGCTATGCTAGTTGCAATACCCATTTGGGAATAGATCAGAGTCGTCGGGATGATTTGGAGTCACTTGGATATGTTCTTCTGTACTTCTTAAGAGGAAGCCTTCCTTGGCAGGGTCTAAAAGCTGCAACAAAGAAGCAAAAATATGATAAGATTTGTGAGAAGAAGCTATCAACTCCCATTGAGGTCTTATGTAAATCGCATCCTGTGGAGTTTGCATCCTACTTCCATTATTGCCACTCTTTAACATTTGATCAACGCCCTGATTATGCATTCTTGAAGCGCCTGTTTCGTGATTTATTTAGCCGCGAAGGATACGAGTTTGACTATATATTTGACTGGACAATTCTAAAATACCAGCAGGCACAGCATACAAGGACTCAGGGTCAATCATCTAAACCGCAGCCACCTGTAGTTGTCTCTGGTCGAGAAATTCCTAGCGGTCAAGCAGTGCCTGCGGATGCGATGAGGCTTAAAGGCATTCATGATGTTCCTAGACCAGCTGAGGTCACAGCAAATAAGCGGCCAAGCAATCATGCGCATCCAGATGTTCATATGCAATATAGATCATCAACAGCCCAGAATTCGGGTGCTAATAATACTGTTGAGAAACACAATGCGAGTAATGCACCTATGCCACCTACTGCATTTTCTCTCAGAAGACATTTTCAAAAACCAGAGGGGCCGGTTGAAAATGCAAACTTAGGCCGTAGAGTTGGGAATCAAAATGGTGCTTCAAGCAGTTGGATGTCATCAGTAAACCAAATTTCTTCAGCAAACTATAGATCTGAAGGAGATTGCTCAGAGCATGTGTCTTTCGGTAGCAAATGCCATGAAGTTTGGGTTGCTTGTTTCTCCCATGACAGATTGCATAACACATGTGAAAACTTCAACTGGCAAAACTTGAACACACAGGATGTGCTTGGACTTCACACCACAGGATGGAGTTTCCGCGTGCATAGGTGGCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04310 ko04340 ko04341 ko04390 ko04391 ko04392 ko04540 ko04710 ko04711 map04068 map04310 map04340 map04341 map04390 map04391 map04392 map04540 map04710 map04711
Pfam Domains
Protein Families
Pkinase

Protein Analysis

501

Amino Acids

57.08

Weight (kDa)

9.32

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 9 - 227 8.1e-27 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 10 - 269 8.2e-13 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 110 - 206 3.1e-08 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016276)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g35730 FvH4_2g35730 FvH4_2g35730
malus_domestica MD08G1115500.v1.1 MD15G1094700.v1.1
prunus_persica Prupe.1G449900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306051
rosa_laevigata RLG00000010828
rosa_roxburghii Rroxscaffold_7G00162090
rosa_rugosa Rorug06G0346600 Rorug06G0346700
rosa_samantha Rh6BG442100 Rh6CG473200 Rh6DG459500
rosa_wichuraiana Rw6G039990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 168
AasI GACNNNNNNGTC 1 cut(s) 233
Acc16I TGCGCA 1 cut(s) 1068
AccB7I CCANNNNNTGG 1 cut(s) 1470
AccBSI CCGCTC 1 cut(s) 51
AccII CGCG 2 cut(s) 837, 1482
AciI CCGC 9 cut(s) 51, 107, 212, 835, 929, 969, 986, 1051, 1480
AclWI GGATC 2 cut(s) 17, 61
AcoI YGGCCR 1 cut(s) 1052
AcsI RAATTY 5 cut(s) 100, 171, 960, 1111, 1293
AcuI CTGAAG 3 cut(s) 326, 1284, 1338
AfaI GTAC 6 cut(s) 26, 152, 284, 357, 500, 614
AfiI CCNNNNNNNGG 3 cut(s) 641, 1114, 1470
AflII CTTAAG 1 cut(s) 619
AflIII ACRYGT 1 cut(s) 1406
AgsI TTSAA 8 cut(s) 119, 269, 808, 1199, 1220, 1266, 1420, 1435
AhdI GACNNNNNGTC 1 cut(s) 912
AjuI GAANNNNNNNTTGG 2 cut(s) 619, 651
AluBI AGCT 5 cut(s) 163, 516, 653, 697, 1031
AluI AGCT 5 cut(s) 163, 516, 653, 697, 1031
Alw26I GTCTC 3 cut(s) 83, 375, 952
AlwI GGATC 2 cut(s) 17, 61
AlwNI CAGNNNCTG 2 cut(s) 527, 983
AoxI GGCC 3 cut(s) 1052, 1211, 1234
ApeKI GCWGC 2 cut(s) 653, 931
ApoI RAATTY 5 cut(s) 100, 171, 960, 1111, 1293
ArsI GACNNNNNNTTYG 2 cut(s) 845, 877
AspLEI GCGC 2 cut(s) 813, 1069
AspS9I GGNCC 3 cut(s) 257, 472, 1211
AvaII GGWCC 2 cut(s) 257, 472
BbsI GAAGAC 3 cut(s) 276, 314, 1195
BbvCI CCTCAGC 1 cut(s) 1032
BbvI GCAGC 2 cut(s) 640, 943
BccI CCATC 2 cut(s) 201, 1464
BceAI ACGGC 1 cut(s) 1221
BciVI GTATCC 1 cut(s) 836
BclI TGATCA 2 cut(s) 328, 781
BcoDI GTCTC 3 cut(s) 83, 375, 952
BfaI CTAG 3 cut(s) 540, 966, 1023
BfmI CTRYAG 3 cut(s) 176, 939, 1309
BfoI RGCGCY 1 cut(s) 814
BfrI CTTAAG 1 cut(s) 619
BfuI GTATCC 1 cut(s) 836
BglII AGATCT 1 cut(s) 1313
BisI GCNGC 5 cut(s) 108, 654, 835, 932, 1052
BlsI GCNGC 5 cut(s) 109, 655, 836, 933, 1053
Bme18I GGWCC 2 cut(s) 257, 472
BmeRI GACNNNNNGTC 1 cut(s) 912
BmgT120I GGNCC 3 cut(s) 257, 472, 1211
BmiI GGNNCC 3 cut(s) 258, 473, 1212
BmsI GCATC 4 cut(s) 739, 755, 979, 1078
BpiI GAAGAC 3 cut(s) 276, 314, 1195
Bpu10I CCTNAGC 1 cut(s) 1032
BsaJI CCNNGG 1 cut(s) 635
Bsc4I CCNNNNNNNGG 3 cut(s) 641, 1114, 1470
Bse118I RCCGGY 2 cut(s) 486, 1213
Bse1I ACTGG 3 cut(s) 97, 872, 1427
BseDI CCNNGG 1 cut(s) 635
BseGI GGATG 9 cut(s) 136, 586, 730, 746, 994, 1069, 1281, 1450, 1475
BseLI CCNNNNNNNGG 3 cut(s) 641, 1114, 1470
BseMII CTCAG 4 cut(s) 923, 1023, 1198, 1344
BseNI ACTGG 3 cut(s) 97, 872, 1427
BseRI GAGGAG 1 cut(s) 307
BseXI GCAGC 2 cut(s) 640, 943
BseYI CCCAGC 1 cut(s) 253
BsgI GTGCAG 1 cut(s) 513
Bsh1236I CGCG 2 cut(s) 837, 1482
BshFI GGCC 3 cut(s) 1054, 1213, 1236
BsiSI CCGG 2 cut(s) 487, 1214
BslFI GGGAC 3 cut(s) 239, 270, 485
BslI CCNNNNNNNGG 3 cut(s) 641, 1114, 1470
BsmAI GTCTC 3 cut(s) 83, 375, 952
BsmFI GGGAC 3 cut(s) 239, 270, 485
BsmI GAATGC 2 cut(s) 800, 1008
BsnI GGCC 3 cut(s) 1054, 1213, 1236
Bsp1407I TGTACA 1 cut(s) 355
Bsp143I GATC 7 cut(s) 9, 53, 328, 565, 781, 1094, 1313
BspACI CCGC 9 cut(s) 51, 107, 212, 835, 929, 969, 986, 1051, 1480
BspANI GGCC 3 cut(s) 1054, 1213, 1236
BspCNI CTCAG 4 cut(s) 922, 1024, 1197, 1343
BspFNI CGCG 2 cut(s) 837, 1482
BspHI TCATGA 1 cut(s) 1012
BspLI GGNNCC 3 cut(s) 258, 473, 1212
BspPI GGATC 2 cut(s) 17, 61
BspTI CTTAAG 1 cut(s) 619
BsrBI CCGCTC 1 cut(s) 51
BsrFI RCCGGY 2 cut(s) 486, 1213
BsrGI TGTACA 1 cut(s) 355
BsrI ACTGG 3 cut(s) 97, 872, 1427
BssAI RCCGGY 2 cut(s) 486, 1213
BssECI CCNNGG 1 cut(s) 635
BssMI GATC 7 cut(s) 9, 53, 328, 565, 781, 1094, 1313
BssT1I CCWWGG 1 cut(s) 635
Bst4CI ACNGT 3 cut(s) 287, 313, 1132
BstAFI CTTAAG 1 cut(s) 619
BstAPI GCANNNNNTGC 2 cut(s) 536, 983
BstAUI TGTACA 1 cut(s) 355
BstC8I GCNNGC 5 cut(s) 199, 532, 891, 984, 1484
BstDEI CTNAG 5 cut(s) 909, 1032, 1184, 1231, 1330
BstF5I GGATG 9 cut(s) 136, 586, 730, 746, 994, 1069, 1281, 1450, 1475
BstFNI CGCG 2 cut(s) 837, 1482
BstH2I RGCGCY 1 cut(s) 814
BstHHI GCGC 2 cut(s) 813, 1069
BstKTI GATC 7 cut(s) 12, 56, 331, 568, 784, 1097, 1316
BstMAI GTCTC 3 cut(s) 83, 375, 952
BstMBI GATC 7 cut(s) 9, 53, 328, 565, 781, 1094, 1313
BstMWI GCNNNNNNNGC 5 cut(s) 536, 895, 983, 1154, 1492
BstNSI RCATGY 2 cut(s) 1340, 1410
BstSFI CTRYAG 3 cut(s) 176, 939, 1309
BstUI CGCG 2 cut(s) 837, 1482
BstV1I GCAGC 2 cut(s) 640, 943
BstV2I GAAGAC 3 cut(s) 276, 314, 1195
BstX2I RGATCY 1 cut(s) 1313
BstYI RGATCY 1 cut(s) 1313
BsuI GTATCC 1 cut(s) 836
BsuRI GGCC 3 cut(s) 1054, 1213, 1236
BtgZI GCGATG 1 cut(s) 1007
BtsCI GGATG 9 cut(s) 136, 586, 730, 746, 994, 1069, 1281, 1450, 1475
BtsI GCAGTG 1 cut(s) 984
BtsIMutI CAGTG 2 cut(s) 302, 984
Cac8I GCNNGC 5 cut(s) 199, 532, 891, 984, 1484
CaiI CAGNNNCTG 2 cut(s) 527, 983
CciI TCATGA 1 cut(s) 1012
CfoI GCGC 2 cut(s) 813, 1069
Cfr10I RCCGGY 2 cut(s) 486, 1213
Cfr13I GGNCC 3 cut(s) 257, 472, 1211
Csp6I GTAC 6 cut(s) 25, 151, 283, 356, 499, 613
CspCI CAANNNNNGTGG 2 cut(s) 129, 164
CviAII CATG 7 cut(s) 406, 1013, 1064, 1337, 1361, 1388, 1407
CviQI GTAC 6 cut(s) 25, 151, 283, 356, 499, 613
DdeI CTNAG 5 cut(s) 909, 1032, 1184, 1231, 1330
DpnI GATC 7 cut(s) 11, 55, 330, 567, 783, 1096, 1315
DpnII GATC 7 cut(s) 9, 53, 328, 565, 781, 1094, 1313
DrdI GACNNNNNNGTC 1 cut(s) 233
DriI GACNNNNNGTC 1 cut(s) 912
DseDI GACNNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 1 cut(s) 1052
Eam1105I GACNNNNNGTC 1 cut(s) 912
Eco130I CCWWGG 1 cut(s) 635
Eco47I GGWCC 2 cut(s) 257, 472
Eco57I CTGAAG 3 cut(s) 326, 1284, 1338
EcoRI GAATTC 1 cut(s) 1111
EcoT14I CCWWGG 1 cut(s) 635
EcoT22I ATGCAT 1 cut(s) 802
ErhI CCWWGG 1 cut(s) 635
FaeI CATG 7 cut(s) 409, 1016, 1067, 1340, 1364, 1391, 1410
FalI AAGNNNNNCTT 4 cut(s) 299, 331, 619, 651
FaqI GGGAC 3 cut(s) 239, 270, 485
FatI CATG 7 cut(s) 405, 1012, 1063, 1336, 1360, 1387, 1406
FauI CCCGC 1 cut(s) 205
FauNDI CATATG 1 cut(s) 1083
FbaI TGATCA 2 cut(s) 328, 781
Fnu4HI GCNGC 5 cut(s) 108, 654, 835, 932, 1052
FokI GGATG 9 cut(s) 123, 593, 717, 733, 1001, 1056, 1288, 1457, 1482
Fsp4HI GCNGC 5 cut(s) 108, 654, 835, 932, 1052
FspAI RTGCGCAY 1 cut(s) 1068
FspBI CTAG 3 cut(s) 540, 966, 1023
FspI TGCGCA 1 cut(s) 1068
GlaI GCGC 2 cut(s) 812, 1068
GluI GCNGC 5 cut(s) 108, 654, 835, 932, 1052
GsaI CCCAGC 1 cut(s) 257
HaeII RGCGCY 1 cut(s) 814
HaeIII GGCC 3 cut(s) 1054, 1213, 1236
HapII CCGG 2 cut(s) 487, 1214
HhaI GCGC 2 cut(s) 813, 1069
Hin1II CATG 7 cut(s) 409, 1016, 1067, 1340, 1364, 1391, 1410
Hin6I GCGC 2 cut(s) 811, 1067
HinP1I GCGC 2 cut(s) 811, 1067
HindIII AAGCTT 2 cut(s) 161, 514
HinfI GANTC 5 cut(s) 262, 571, 590, 907, 1249
HpaII CCGG 2 cut(s) 487, 1214
Hpy166II GTNNAC 2 cut(s) 283, 1288
Hpy188I TCNGA 5 cut(s) 333, 570, 1187, 1318, 1333
Hpy188III TCNNGA 8 cut(s) 266, 470, 578, 805, 821, 956, 1013, 1073
Hpy8I GTNNAC 2 cut(s) 283, 1288
Hpy99I CGWCG 1 cut(s) 579
HpyAV CCTTC 3 cut(s) 641, 833, 1313
HpyCH4III ACNGT 3 cut(s) 287, 313, 1132
HpyF10VI GCNNNNNNNGC 5 cut(s) 536, 895, 983, 1154, 1492
HpyF3I CTNAG 5 cut(s) 909, 1032, 1184, 1231, 1330
Hsp92II CATG 7 cut(s) 409, 1016, 1067, 1340, 1364, 1391, 1410
HspAI GCGC 2 cut(s) 811, 1067
Ksp22I TGATCA 2 cut(s) 328, 781
Kzo9I GATC 7 cut(s) 9, 53, 328, 565, 781, 1094, 1313
LmnI GCTCC 1 cut(s) 48
Lsp1109I GCAGC 2 cut(s) 640, 943
LweI GCATC 4 cut(s) 739, 755, 979, 1078
MaeI CTAG 3 cut(s) 540, 966, 1023
MaeIII GTNAC 2 cut(s) 591, 1036
MalI GATC 7 cut(s) 11, 55, 330, 567, 783, 1096, 1315
MbiI CCGCTC 1 cut(s) 51
MboI GATC 7 cut(s) 9, 53, 328, 565, 781, 1094, 1313
MboII GAAGA 7 cut(s) 124, 281, 319, 599, 703, 1200, 1290
MflI RGATCY 1 cut(s) 1313
MluCI AATT 9 cut(s) 29, 42, 65, 100, 171, 873, 960, 1111, 1293
MlyI GAGTC 4 cut(s) 271, 580, 599, 901
MmeI TCCRAC 1 cut(s) 1253
Mph1103I ATGCAT 1 cut(s) 802
MseI TTAA 5 cut(s) 317, 518, 620, 773, 1002
MslI CAYNNNNRTG 1 cut(s) 1074
MspA1I CMGCKG 1 cut(s) 1031
MspCI CTTAAG 1 cut(s) 619
MspI CCGG 2 cut(s) 487, 1214
Mva1269I GAATGC 2 cut(s) 800, 1008
MvnI CGCG 2 cut(s) 837, 1482
MwoI GCNNNNNNNGC 5 cut(s) 536, 895, 983, 1154, 1492
NdeI CATATG 1 cut(s) 1083
NdeII GATC 7 cut(s) 9, 53, 328, 565, 781, 1094, 1313
NlaIII CATG 7 cut(s) 409, 1016, 1067, 1340, 1364, 1391, 1410
NlaIV GGNNCC 3 cut(s) 258, 473, 1212
NmuCI GTSAC 2 cut(s) 591, 1036
NsbI TGCGCA 1 cut(s) 1068
NsiI ATGCAT 1 cut(s) 802
NspI RCATGY 2 cut(s) 1340, 1410
PagI TCATGA 1 cut(s) 1012
PciI ACATGT 1 cut(s) 1406
PctI GAATGC 2 cut(s) 800, 1008
PfeI GAWTC 1 cut(s) 1249
PflFI GACNNNGTC 1 cut(s) 261
PflMI CCANNNNNTGG 1 cut(s) 1470
PkrI GCNGC 5 cut(s) 109, 655, 836, 933, 1053
PleI GAGTC 4 cut(s) 270, 579, 598, 901
PpsI GAGTC 4 cut(s) 270, 579, 598, 901
PscI ACATGT 1 cut(s) 1406
PsiI TTATAA 1 cut(s) 168
PspFI CCCAGC 1 cut(s) 253
PspN4I GGNNCC 3 cut(s) 258, 473, 1212
PspPI GGNCC 3 cut(s) 257, 472, 1211
PstNI CAGNNNCTG 2 cut(s) 527, 983
PsuI RGATCY 1 cut(s) 1313
PsyI GACNNNGTC 1 cut(s) 261
PvuII CAGCTG 1 cut(s) 1031
RsaI GTAC 6 cut(s) 26, 152, 284, 357, 500, 614
RsaNI GTAC 6 cut(s) 25, 151, 283, 356, 499, 613
RseI CAYNNNNRTG 1 cut(s) 1074
SaqAI TTAA 5 cut(s) 317, 518, 620, 773, 1002
SatI GCNGC 5 cut(s) 108, 654, 835, 932, 1052
Sau3AI GATC 7 cut(s) 9, 53, 328, 565, 781, 1094, 1313
Sau96I GGNCC 3 cut(s) 257, 472, 1211
SchI GAGTC 4 cut(s) 271, 580, 599, 901
SfaNI GCATC 4 cut(s) 739, 755, 979, 1078
SfcI CTRYAG 3 cut(s) 176, 939, 1309
SinI GGWCC 2 cut(s) 257, 472
SmiMI CAYNNNNRTG 1 cut(s) 1074
SmlI CTYRAG 1 cut(s) 619
SmoI CTYRAG 1 cut(s) 619
Sse9I AATT 9 cut(s) 29, 42, 65, 100, 171, 873, 960, 1111, 1293
SsiI CCGC 9 cut(s) 51, 107, 212, 835, 929, 969, 986, 1051, 1480
SspMI CTAG 3 cut(s) 540, 966, 1023
StyI CCWWGG 1 cut(s) 635
TaaI ACNGT 3 cut(s) 287, 313, 1132
TaqI TCGA 1 cut(s) 955
TaqII GACCGA 1 cut(s) 274
TasI AATT 9 cut(s) 29, 42, 65, 100, 171, 873, 960, 1111, 1293
TatI WGTACW 4 cut(s) 24, 282, 355, 612
TauI GCSGC 3 cut(s) 110, 837, 1054
TfiI GAWTC 1 cut(s) 1249
Tru1I TTAA 5 cut(s) 317, 518, 620, 773, 1002
Tru9I TTAA 5 cut(s) 317, 518, 620, 773, 1002
TscAI CASTG 2 cut(s) 309, 984
TseFI GTSAC 2 cut(s) 591, 1036
TseI GCWGC 2 cut(s) 653, 931
Tsp45I GTSAC 2 cut(s) 591, 1036
TspDTI ATGAA 3 cut(s) 1001, 1070, 1377
TspGWI ACGGA 1 cut(s) 486
TspRI CASTG 2 cut(s) 309, 984
Tth111I GACNNNGTC 1 cut(s) 261
Van91I CCANNNNNTGG 1 cut(s) 1470
Vha464I CTTAAG 1 cut(s) 619
VpaK11BI GGWCC 2 cut(s) 257, 472
XapI RAATTY 5 cut(s) 100, 171, 960, 1111, 1293
XceI RCATGY 2 cut(s) 1340, 1410
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XspI CTAG 3 cut(s) 540, 966, 1023
Zsp2I ATGCAT 1 cut(s) 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.