Rroxscaffold_7G00162090

Casein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0016276
Physical Location & Seq
Reverse (-)
4721960 .. 4729963
8004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00162090.1

Sequence Viewer

Length: 2577 bp
ATGGAGAGGATTATCGGAGGAAAGTTTAAGCTAGGTCGCAAAATTGGGAGCGGTTCTTTTGGTGAAATATACCTCGCTACTCATATGGAGACTGGAGAGACCTTGGCGGCCAAGATTGAAAGTAACAAGACGAAGCATTTACAGCTTATGTATGAGGCCAAGCTGTATAAGGTTCTACAGGGAGGAAGCGGCATTGCAAGCATGAAGTGGGCGGGGTTTGATCATGATGATAACGTTGTCATCATAGACTTGTTGGGGCCGAGTCTTGAAGACTTATTTGTATACTGTGGGAGGAAGTTTTCACTGAAAACAGTCTTAATGCTGGCCGAACAGATGATTACGAGAATAGAGTATGTGCATTCGAAAGGGTTTTTGCATAGAGACATCAAACCAGACAATTATCTCATGGGTCTTGGCAGGAAAGCAAATCAGGTTTACATCATTGACTTTGGACTTGCAAAAAGATATCGGGACCCAACTACAAATCGGCACATTCCATACAGAGAAAACAAAAGCTTAACAGGAACTGCACGATATGCTAGTTGCAATACCCATTTGGGAATTGATCAGAGTCGTCGGGATGATTTGGAGTCACTTGGCTATGTTCTTCTGTACTTCTTAAGAGGAAGCCTTCCTTGGCAGGGTCTTAAAGCTGCAACGAAGAAGCAAAAATATGATAAGATTTGTGAGAAGAAGTTATCAACTCCCATTGAGGTGCTATGTAAGGCGCATCCTGTGGAGTTTGCATCCTACTTCCACTATTGCCATTCCTTAACATTTGATCAACGTCCGGATTACTCATTCTTGAAGCGTCTGTTTCGTGACTTATTCACCCGCGAAGGATTTGAGTTTGATTATGTATTTGACTGGACAATCCTAAAGTACCAGCAGGCGCAACAGACAAGGGCTCATGGTCAATCAACTCCAGCTGTAGCGACAAATGGTCAAGTAGTGACTAGCAGTCGAGCAGTGCCTATGGATGTGAGAAATCAGGGAGTTGATGATGTTTCTAGACAAGCTCAGCCTCGAGAACATAAAAGATCAAGTCATCTTGTTCATATTCACAGGCCATTTAGATCATCTGCAGTCCAGAATTTGAGTGCTAATAACTCTATTGAAAAACATAATACGGGCAATGCTCTCAAAAGACATTTACCAAAACCTGAGGGGCCAGCTGAAATTGCTAATGTAGGTCGCCAACACGCTACAGATGTAAAAGAATTTACTCGGAGCATGCATCTTGCTGTAGCAAATGACTTGTCTACAAAGCTTGGAGTTTTCATGTGCATAGGTGGAAAGGATTGGCCGAGCCCGTTTAATGTTGTGTGGATGACGTCCCTGGATGTTCATCTGGATGAAGTTTCAAGTTTGAACCTTAAGTACCCAGATCTTGATAGGAGATGGGTTATAAAGGATGAAAGGAAGGAACTTCATTGGAACTGTCAATTCAAGAGGAGCCTTGGGAGGGTTTTAGGGGATCTTGGTGTGCCTCCTCCAATTTATCTTGATGGGAATACTGTGAAAGCAGTTACATGGGAAACAAAGTGTAAAGAAACCAGCTTTACCAGTTTGAAATGCTCGACGAATCGACATGGGTTGGAGAACATAGACGAAAATGGTGGCACCAGTATTGGGGCGGCGGGTTTATGTGCCCCGCCACCCGAAACTCCGACGGAGTCCATGGAGTTTCTGGCGAGATCATGGAGCCTCTCGGCCATGGAGATCTCCAAAGCTCTTTCCCACTCACATGTTGCTTCTAAGAGTCTTGAGAATAAGTTAGCATTCTCTTATGATGGTACTGCTGAAACTCGCCATTCAAGTTCCATGCGTTTGAGCGAATCATCTCTGCCTGGTGGAGTTAGCCCTCCGATTTCGCCAAGAAACAGCGATGAAATGAAGGAGTTGTTTTTACTACATCATGCACTTCATCCAGAGTTCCTTTCCAGTCAACAACAATTGATCAATAATGGGCTATACAAGAGCATGTTGAGAGTTTCTGTGGCTGGAGTTGCTGCTGCTGTAGCAGCACTCGCAGCTGCAACTGCATTACCTGAAACTGAAACATCAGCTGCCAGTGCCCACCACAAAGCAGCAGTATCCGAGAGGACTGAAATTTCGACTGCACTAGCATCTGCAGCAGCTCTGGTAGCTTCACGCTGCATTGAGATTGCAGAGGAGATGGGAGCTGATCACGACCATATTCTATCAGTTGTCAGTTCTGCAGTTAACGTGAGGACTAATGGAGACATCATGACCTTAACAGCTGGAGCTGCCACAGGTGATCTCCACTGGAAACAAGTTTCTTTCAACACCAACTCAAATGGGCAGGTTGTAGCCAAAATGAAAAGCAAACACATGGCGGGAACATTTACAAAGAAGAAGACATGTGTAGTCTCTGAAGTCTACTGTGACATAGCAGCGTGGCCAGGAAGGGAGAGGGAAGATTGCAGCAAACCAAGGGCATACTTTGGCATCAAAACAGCTGACAGGACAATAGAATTTGAATGCAGAAGTAAAAGGGACAGGCATATGTGGACTGAGGGGATTCAGAATATGTTGAATTTCATACCAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04310 ko04340 ko04341 ko04390 ko04391 ko04392 ko04540 ko04710 ko04711 map04068 map04310 map04340 map04341 map04390 map04391 map04392 map04540 map04710 map04711
Pfam Domains
Protein Families
Pkinase

Protein Analysis

858

Amino Acids

95.64

Weight (kDa)

8.92

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 10 - 227 1.4e-26 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 10 - 269 1.3e-13 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 113 - 206 1.6e-07 Fungal protein kinase
Auxin_canalis PF05703 554 - 652 2e-17 Auxin canalisation
Auxin_canalis PF05703 665 - 759 5e-30 Auxin canalisation
PH_2 PF08458 757 - 854 8.7e-38 Plant pleckstrin homology-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016276)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g35730 FvH4_2g35730 FvH4_2g35730
malus_domestica MD08G1115500.v1.1 MD15G1094700.v1.1
prunus_persica Prupe.1G449900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306051
rosa_laevigata RLG00000010828
rosa_roxburghii Rroxscaffold_7G00162090
rosa_rugosa Rorug06G0346600 Rorug06G0346700
rosa_samantha Rh6BG442100 Rh6CG473200 Rh6DG459500
rosa_wichuraiana Rw6G039990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1409
AatII GACGTC 1 cut(s) 1337
Acc36I ACCTGC 1 cut(s) 2317
AccB1I GGYRCC 1 cut(s) 1622
AccBSI CCGCTC 1 cut(s) 51
AccI GTMKAC 3 cut(s) 282, 1262, 2403
AccII CGCG 1 cut(s) 837
AccIII TCCGGA 1 cut(s) 790
AciI CCGC 9 cut(s) 51, 107, 189, 212, 835, 1637, 1640, 1655, 2360
AclI AACGTT 1 cut(s) 234
AclWI GGATC 1 cut(s) 1485
AcoI YGGCCR 5 cut(s) 108, 324, 1304, 1713, 2423
AcsI RAATTY 5 cut(s) 1093, 1220, 2112, 2498, 2560
AcuI CTGAAG 1 cut(s) 2418
AcyI GRCGYC 1 cut(s) 1334
AfaI GTAC 4 cut(s) 614, 884, 1382, 1798
AfiI CCNNNNNNNGG 3 cut(s) 641, 1465, 1632
AflII CTTAAG 2 cut(s) 619, 1376
AflIII ACRYGT 2 cut(s) 1747, 2384
AhdI GACNNNNNGTC 2 cut(s) 942, 960
AjnI CCWGG 3 cut(s) 1338, 1849, 2425
AjuI GAANNNNNNNTTGG 2 cut(s) 619, 651
Alw26I GTCTC 5 cut(s) 83, 92, 375, 2238, 2398
AlwI GGATC 1 cut(s) 1485
AlwNI CAGNNNCTG 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 1026
Aor13HI TCCGGA 1 cut(s) 790
AoxI GGCC 9 cut(s) 108, 156, 257, 324, 1067, 1169, 1304, 1713, 2423
ApoI RAATTY 5 cut(s) 1093, 1220, 2112, 2498, 2560
ArsI GACNNNNNNTTYG 4 cut(s) 1244, 1276, 2098, 2130
AspLEI GCGC 2 cut(s) 730, 895
AspS9I GGNCC 3 cut(s) 257, 472, 1169
AsuHPI GGTGA 3 cut(s) 74, 823, 2291
AsuII TTCGAA 1 cut(s) 362
AvaI CYCGRG 1 cut(s) 1026
AvaII GGWCC 1 cut(s) 472
AxyI CCTNAGG 1 cut(s) 1164
BaeGI GKGCMC 2 cut(s) 1654, 2080
BalI TGGCCA 1 cut(s) 2425
BanI GGYRCC 1 cut(s) 1622
BanII GRGCYC 2 cut(s) 910, 1313
BbsI GAAGAC 2 cut(s) 276, 2387
BccI CCATC 4 cut(s) 1395, 1502, 1787, 2173
BciT130I CCWGG 3 cut(s) 1340, 1851, 2427
BciVI GTATCC 1 cut(s) 2107
BclI TGATCA 5 cut(s) 220, 565, 781, 1959, 2188
BcoDI GTCTC 5 cut(s) 83, 92, 375, 2238, 2398
BfaI CTAG 5 cut(s) 32, 540, 957, 1011, 2126
BfmI CTRYAG 8 cut(s) 176, 930, 1083, 1206, 1245, 2019, 2133, 2220
BfrI CTTAAG 2 cut(s) 619, 1376
BfuAI ACCTGC 1 cut(s) 2317
BfuI GTATCC 1 cut(s) 2107
BglII AGATCT 2 cut(s) 1387, 1722
BlpI GCTNAGC 1 cut(s) 1020
Bme1390I CCNGG 3 cut(s) 1340, 1851, 2427
Bme18I GGWCC 1 cut(s) 472
BmeRI GACNNNNNGTC 2 cut(s) 942, 960
BmeT110I CYCGRG 1 cut(s) 1026
BmgT120I GGNCC 3 cut(s) 257, 472, 1169
BmiI GGNNCC 7 cut(s) 258, 473, 474, 1170, 1457, 1624, 1706
BmrFI CCNGG 3 cut(s) 1340, 1851, 2427
BmsI GCATC 5 cut(s) 739, 755, 1246, 2138, 2481
BpiI GAAGAC 2 cut(s) 276, 2387
BpmI CTGGAG 4 cut(s) 114, 909, 2025, 2286
Bpu1102I GCTNAGC 1 cut(s) 1020
Bpu14I TTCGAA 1 cut(s) 362
BpuEI CTTGAG 1 cut(s) 1787
BsaBI GATNNNNATC 1 cut(s) 1347
BsaHI GRCGYC 1 cut(s) 1334
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 7 cut(s) 102, 635, 1338, 1459, 1680, 1716, 2456
BsaWI WCCGGW 1 cut(s) 790
Bsc4I CCNNNNNNNGG 3 cut(s) 641, 1465, 1632
Bse1I ACTGG 7 cut(s) 97, 872, 1566, 1626, 1944, 2073, 2294
Bse21I CCTNAGG 1 cut(s) 1164
Bse3DI GCAATG 2 cut(s) 192, 1141
Bse8I GATNNNNATC 1 cut(s) 1347
BseAI TCCGGA 1 cut(s) 790
BseBI CCWGG 3 cut(s) 1340, 1851, 2427
BseDI CCNNGG 7 cut(s) 102, 635, 1338, 1459, 1680, 1716, 2456
BseGI GGATG 9 cut(s) 586, 730, 746, 985, 1335, 1348, 1360, 1420, 1927
BseJI GATNNNNATC 1 cut(s) 1347
BseLI CCNNNNNNNGG 3 cut(s) 641, 1465, 1632
BseMI GCAATG 2 cut(s) 192, 1141
BseMII CTCAG 3 cut(s) 1034, 1155, 2529
BseNI ACTGG 7 cut(s) 97, 872, 1566, 1626, 1944, 2073, 2294
BseRI GAGGAG 3 cut(s) 1468, 1482, 2189
BseSI GKGCMC 2 cut(s) 1654, 2080
BsgI GTGCAG 2 cut(s) 513, 2106
Bsh1236I CGCG 1 cut(s) 837
BshFI GGCC 9 cut(s) 110, 158, 259, 326, 1069, 1171, 1306, 1715, 2425
BshNI GGYRCC 1 cut(s) 1622
BsiHKCI CYCGRG 1 cut(s) 1026
BsiSI CCGG 1 cut(s) 791
BslFI GGGAC 3 cut(s) 485, 1321, 2534
BslI CCNNNNNNNGG 3 cut(s) 641, 1465, 1632
BsmAI GTCTC 5 cut(s) 83, 92, 375, 2238, 2398
BsmFI GGGAC 3 cut(s) 485, 1321, 2534
BsmI GAATGC 3 cut(s) 358, 1781, 2510
BsnI GGCC 9 cut(s) 110, 158, 259, 326, 1069, 1171, 1306, 1715, 2425
Bso31I GGTCTC 1 cut(s) 92
BsoBI CYCGRG 1 cut(s) 1026
Bsp119I TTCGAA 1 cut(s) 362
Bsp1286I GDGCHC 4 cut(s) 910, 1313, 1654, 2080
Bsp13I TCCGGA 1 cut(s) 790
Bsp1720I GCTNAGC 1 cut(s) 1020
Bsp19I CCATGG 2 cut(s) 1680, 1716
BspACI CCGC 9 cut(s) 51, 107, 189, 212, 835, 1637, 1640, 1655, 2360
BspANI GGCC 9 cut(s) 110, 158, 259, 326, 1069, 1171, 1306, 1715, 2425
BspCNI CTCAG 3 cut(s) 1033, 1156, 2530
BspEI TCCGGA 1 cut(s) 790
BspFNI CGCG 1 cut(s) 837
BspHI TCATGA 2 cut(s) 223, 2250
BspLI GGNNCC 7 cut(s) 258, 473, 474, 1170, 1457, 1624, 1706
BspMAI CTGCAG 3 cut(s) 1087, 2137, 2224
BspMI ACCTGC 1 cut(s) 2317
BspPI GGATC 1 cut(s) 1485
BspT104I TTCGAA 1 cut(s) 362
BspT107I GGYRCC 1 cut(s) 1622
BspTI CTTAAG 2 cut(s) 619, 1376
BspTNI GGTCTC 1 cut(s) 92
BsrBI CCGCTC 1 cut(s) 51
BsrDI GCAATG 2 cut(s) 192, 1141
BsrI ACTGG 7 cut(s) 97, 872, 1566, 1626, 1944, 2073, 2294
BssECI CCNNGG 7 cut(s) 102, 635, 1338, 1459, 1680, 1716, 2456
BssNAI GTATAC 1 cut(s) 283
BssNI GRCGYC 1 cut(s) 1334
BssT1I CCWWGG 6 cut(s) 102, 635, 1459, 1680, 1716, 2456
Bst1107I GTATAC 1 cut(s) 283
Bst2UI CCWGG 3 cut(s) 1340, 1851, 2427
Bst4CI ACNGT 5 cut(s) 287, 313, 1442, 1519, 2408
BstACI GRCGYC 1 cut(s) 1334
BstAFI CTTAAG 2 cut(s) 619, 1376
BstAPI GCANNNNNTGC 1 cut(s) 536
BstBI TTCGAA 1 cut(s) 362
BstC8I GCNNGC 5 cut(s) 199, 324, 891, 1173, 1235
BstDEI CTNAG 4 cut(s) 1020, 1164, 1758, 2538
BstDSI CCRYGG 2 cut(s) 1680, 1716
BstF5I GGATG 9 cut(s) 586, 730, 746, 985, 1335, 1348, 1360, 1420, 1927
BstFNI CGCG 1 cut(s) 837
BstHHI GCGC 2 cut(s) 730, 895
BstMAI GTCTC 5 cut(s) 83, 92, 375, 2238, 2398
BstNI CCWGG 3 cut(s) 1340, 1851, 2427
BstNSI RCATGY 4 cut(s) 1237, 1751, 1987, 2388
BstSCI CCNGG 3 cut(s) 1338, 1849, 2425
BstSFI CTRYAG 8 cut(s) 176, 930, 1083, 1206, 1245, 2019, 2133, 2220
BstSLI GKGCMC 2 cut(s) 1654, 2080
BstUI CGCG 1 cut(s) 837
BstV2I GAAGAC 2 cut(s) 276, 2387
BstX2I RGATCY 3 cut(s) 1387, 1477, 1722
BstYI RGATCY 3 cut(s) 1387, 1477, 1722
BstZ17I GTATAC 1 cut(s) 283
Bsu36I CCTNAGG 1 cut(s) 1164
BsuI GTATCC 1 cut(s) 2107
BsuRI GGCC 9 cut(s) 110, 158, 259, 326, 1069, 1171, 1306, 1715, 2425
BtgI CCRYGG 2 cut(s) 1680, 1716
BtgZI GCGATG 1 cut(s) 1902
BtsCI GGATG 9 cut(s) 586, 730, 746, 985, 1335, 1348, 1360, 1420, 1927
BtsI GCAGTG 1 cut(s) 975
BtsIMutI CAGTG 4 cut(s) 302, 975, 2080, 2287
BveI ACCTGC 1 cut(s) 2317
Cac8I GCNNGC 5 cut(s) 199, 324, 891, 1173, 1235
CaiI CAGNNNCTG 1 cut(s) 527
CciI TCATGA 2 cut(s) 223, 2250
CfoI GCGC 2 cut(s) 730, 895
Cfr13I GGNCC 3 cut(s) 257, 472, 1169
CseI GACGC 1 cut(s) 800
Csp6I GTAC 4 cut(s) 613, 883, 1381, 1797
CviQI GTAC 4 cut(s) 613, 883, 1381, 1797
DdeI CTNAG 4 cut(s) 1020, 1164, 1758, 2538
DriI GACNNNNNGTC 2 cut(s) 942, 960
EaeI YGGCCR 5 cut(s) 108, 324, 1304, 1713, 2423
Eam1105I GACNNNNNGTC 2 cut(s) 942, 960
Eco130I CCWWGG 6 cut(s) 102, 635, 1459, 1680, 1716, 2456
Eco24I GRGCYC 2 cut(s) 910, 1313
Eco31I GGTCTC 1 cut(s) 92
Eco32I GATATC 1 cut(s) 467
Eco47I GGWCC 1 cut(s) 472
Eco57I CTGAAG 1 cut(s) 2418
Eco81I CCTNAGG 1 cut(s) 1164
Eco88I CYCGRG 1 cut(s) 1026
EcoO109I RGGNCCY 1 cut(s) 472
EcoRII CCWGG 3 cut(s) 1338, 1849, 2425
EcoRV GATATC 1 cut(s) 467
EcoT14I CCWWGG 6 cut(s) 102, 635, 1459, 1680, 1716, 2456
EcoT22I ATGCAT 1 cut(s) 1239
EcoT38I GRGCYC 2 cut(s) 910, 1313
ErhI CCWWGG 6 cut(s) 102, 635, 1459, 1680, 1716, 2456
FalI AAGNNNNNCTT 2 cut(s) 619, 651
FaqI GGGAC 3 cut(s) 485, 1321, 2534
FauI CCCGC 5 cut(s) 205, 842, 1633, 1662, 2353
FauNDI CATATG 2 cut(s) 84, 2529
FbaI TGATCA 5 cut(s) 220, 565, 781, 1959, 2188
FblI GTMKAC 3 cut(s) 282, 1262, 2403
FokI GGATG 9 cut(s) 593, 717, 733, 992, 1342, 1355, 1367, 1427, 1914
FriOI GRGCYC 2 cut(s) 910, 1313
FspBI CTAG 5 cut(s) 32, 540, 957, 1011, 2126
GlaI GCGC 2 cut(s) 729, 894
GsuI CTGGAG 4 cut(s) 114, 909, 2025, 2286
HaeIII GGCC 9 cut(s) 110, 158, 259, 326, 1069, 1171, 1306, 1715, 2425
HapII CCGG 1 cut(s) 791
HgaI GACGC 1 cut(s) 800
HhaI GCGC 2 cut(s) 730, 895
Hin1I GRCGYC 1 cut(s) 1334
Hin6I GCGC 2 cut(s) 728, 893
HinP1I GCGC 2 cut(s) 728, 893
HincII GTYRAC 2 cut(s) 1949, 2227
HindII GTYRAC 2 cut(s) 1949, 2227
HindIII AAGCTT 2 cut(s) 514, 1268
HinfI GANTC 8 cut(s) 262, 571, 590, 1585, 1676, 1762, 1838, 2545
HpaI GTTAAC 1 cut(s) 2227
HpaII CCGG 1 cut(s) 791
HphI GGTGA 3 cut(s) 74, 823, 2291
Hpy166II GTNNAC 7 cut(s) 283, 436, 1263, 1949, 2227, 2404, 2535
Hpy188I TCNGA 8 cut(s) 17, 570, 1230, 1671, 1869, 2101, 2398, 2550
Hpy8I GTNNAC 7 cut(s) 283, 436, 1263, 1949, 2227, 2404, 2535
Hpy99I CGWCG 3 cut(s) 579, 1585, 1675
HpyAV CCTTC 5 cut(s) 641, 833, 1417, 1891, 2424
HpyCH4III ACNGT 5 cut(s) 287, 313, 1442, 1519, 2408
HpyCH4IV ACGT 4 cut(s) 234, 787, 1334, 2229
HpyF3I CTNAG 4 cut(s) 1020, 1164, 1758, 2538
HpySE526I ACGT 4 cut(s) 234, 787, 1334, 2229
Hsp92I GRCGYC 1 cut(s) 1334
HspAI GCGC 2 cut(s) 728, 893
KflI GGGWCCC 1 cut(s) 472
Kpn2I TCCGGA 1 cut(s) 790
Ksp22I TGATCA 5 cut(s) 220, 565, 781, 1959, 2188
KspAI GTTAAC 1 cut(s) 2227
LmnI GCTCC 6 cut(s) 48, 1230, 1455, 1704, 2183, 2267
LweI GCATC 5 cut(s) 739, 755, 1246, 2138, 2481
MaeI CTAG 5 cut(s) 32, 540, 957, 1011, 2126
MaeII ACGT 4 cut(s) 234, 787, 1334, 2229
MaeIII GTNAC 6 cut(s) 122, 591, 821, 952, 1528, 2408
MbiI CCGCTC 1 cut(s) 51
MboII GAAGA 7 cut(s) 281, 599, 673, 703, 2389, 2392, 2453
MfeI CAATTG 1 cut(s) 1955
MflI RGATCY 3 cut(s) 1387, 1477, 1722
MhlI GDGCHC 4 cut(s) 910, 1313, 1654, 2080
MlsI TGGCCA 1 cut(s) 2425
MluNI TGGCCA 1 cut(s) 2425
MlyI GAGTC 5 cut(s) 271, 580, 599, 1685, 1771
MmeI TCCRAC 2 cut(s) 1578, 1694
Mox20I TGGCCA 1 cut(s) 2425
Mph1103I ATGCAT 1 cut(s) 1239
MroI TCCGGA 1 cut(s) 790
MscI TGGCCA 1 cut(s) 2425
MslI CAYNNNNRTG 3 cut(s) 713, 1353, 1746
Msp20I TGGCCA 1 cut(s) 2425
MspA1I CMGCKG 6 cut(s) 929, 1175, 2036, 2069, 2264, 2483
MspCI CTTAAG 2 cut(s) 619, 1376
MspI CCGG 1 cut(s) 791
MspR9I CCNGG 3 cut(s) 1340, 1851, 2427
MunI CAATTG 1 cut(s) 1955
Mva1269I GAATGC 3 cut(s) 358, 1781, 2510
MvaI CCWGG 3 cut(s) 1340, 1851, 2427
MvnI CGCG 1 cut(s) 837
NcoI CCATGG 2 cut(s) 1680, 1716
NdeI CATATG 2 cut(s) 84, 2529
NlaIV GGNNCC 7 cut(s) 258, 473, 474, 1170, 1457, 1624, 1706
NmeAIII GCCGAG 3 cut(s) 285, 1332, 1691
NmuCI GTSAC 4 cut(s) 591, 821, 952, 2408
NsiI ATGCAT 1 cut(s) 1239
NspI RCATGY 4 cut(s) 1237, 1751, 1987, 2388
NspV TTCGAA 1 cut(s) 362
PaeI GCATGC 1 cut(s) 1237
PaeR7I CTCGAG 1 cut(s) 1026
PagI TCATGA 2 cut(s) 223, 2250
PciI ACATGT 2 cut(s) 1747, 2384
PctI GAATGC 3 cut(s) 358, 1781, 2510
PfeI GAWTC 3 cut(s) 1585, 1838, 2545
PflFI GACNNNGTC 1 cut(s) 1675
PleI GAGTC 5 cut(s) 270, 579, 598, 1684, 1770
PpsI GAGTC 5 cut(s) 270, 579, 598, 1684, 1770
PpuMI RGGWCCY 1 cut(s) 472
PscI ACATGT 2 cut(s) 1747, 2384
PsiI TTATAA 1 cut(s) 1409
Psp1406I AACGTT 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 472
Psp6I CCWGG 3 cut(s) 1338, 1849, 2425
PspGI CCWGG 3 cut(s) 1338, 1849, 2425
PspN4I GGNNCC 7 cut(s) 258, 473, 474, 1170, 1457, 1624, 1706
PspPI GGNCC 3 cut(s) 257, 472, 1169
PspPPI RGGWCCY 1 cut(s) 472
PsrI GAACNNNNNNTAC 2 cut(s) 1364, 1396
PstI CTGCAG 3 cut(s) 1087, 2137, 2224
PstNI CAGNNNCTG 1 cut(s) 527
PsuI RGATCY 3 cut(s) 1387, 1477, 1722
PsyI GACNNNGTC 1 cut(s) 1675
PvuII CAGCTG 6 cut(s) 929, 1175, 2036, 2069, 2264, 2483
RsaI GTAC 4 cut(s) 614, 884, 1382, 1798
RsaNI GTAC 4 cut(s) 613, 883, 1381, 1797
RseI CAYNNNNRTG 3 cut(s) 713, 1353, 1746
Sau96I GGNCC 3 cut(s) 257, 472, 1169
SchI GAGTC 5 cut(s) 271, 580, 599, 1685, 1771
ScrFI CCNGG 3 cut(s) 1340, 1851, 2427
SduI GDGCHC 4 cut(s) 910, 1313, 1654, 2080
SfaNI GCATC 5 cut(s) 739, 755, 1246, 2138, 2481
SfcI CTRYAG 8 cut(s) 176, 930, 1083, 1206, 1245, 2019, 2133, 2220
Sfr274I CTCGAG 1 cut(s) 1026
SfuI TTCGAA 1 cut(s) 362
SinI GGWCC 1 cut(s) 472
SlaI CTCGAG 1 cut(s) 1026
SmiMI CAYNNNNRTG 3 cut(s) 713, 1353, 1746
SmlI CTYRAG 4 cut(s) 619, 1026, 1376, 1766
SmoI CTYRAG 4 cut(s) 619, 1026, 1376, 1766
SphI GCATGC 1 cut(s) 1237
SsiI CCGC 9 cut(s) 51, 107, 189, 212, 835, 1637, 1640, 1655, 2360
SspMI CTAG 5 cut(s) 32, 540, 957, 1011, 2126
StyD4I CCNGG 3 cut(s) 1338, 1849, 2425
StyI CCWWGG 6 cut(s) 102, 635, 1459, 1680, 1716, 2456
TaaI ACNGT 5 cut(s) 287, 313, 1442, 1519, 2408
TaiI ACGT 4 cut(s) 237, 790, 1337, 2232
TaqI TCGA 6 cut(s) 362, 964, 1027, 1580, 1588, 2117
TatI WGTACW 1 cut(s) 612
TauI GCSGC 3 cut(s) 110, 192, 1640
TfiI GAWTC 3 cut(s) 1585, 1838, 2545
TscAI CASTG 4 cut(s) 309, 975, 2080, 2294
TseFI GTSAC 4 cut(s) 591, 821, 952, 2408
Tsp45I GTSAC 4 cut(s) 591, 821, 952, 2408
TspGWI ACGGA 1 cut(s) 1688
TspRI CASTG 4 cut(s) 309, 975, 2080, 2294
Tth111I GACNNNGTC 1 cut(s) 1675
Vha464I CTTAAG 2 cut(s) 619, 1376
VpaK11BI GGWCC 1 cut(s) 472
XapI RAATTY 5 cut(s) 1093, 1220, 2112, 2498, 2560
XbaI TCTAGA 1 cut(s) 1010
XceI RCATGY 4 cut(s) 1237, 1751, 1987, 2388
XcmI CCANNNNNNNNNTGG 1 cut(s) 1687
XhoI CTCGAG 1 cut(s) 1026
XmiI GTMKAC 3 cut(s) 282, 1262, 2403
XspI CTAG 5 cut(s) 32, 540, 957, 1011, 2126
ZraI GACGTC 1 cut(s) 1335
Zsp2I ATGCAT 1 cut(s) 1239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.