RLG00000010828

Casein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016276
Physical Location & Seq
Reverse (-)
4628295 .. 4631367
3073 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010828

Sequence Viewer

Length: 1239 bp
ATGGAGAGGATTATCGGAGGAAAGTTTAAGCTAGGTCGCAAAATTGGGAGCGGTTCTTTTGGTGAAATATACCTCGCTACTCATATGGAGACTGGAGAGACCTTGGCGGCCAAGATTGAAAGTAACAAGACGAAGCATTTACAGCTTATGTATGAGGCCAAGCTGTATAAAGTTCTACAGGGAGGAAGCGGCATTGCAAGCATGAAGTGGGCGGGGTTTGATCATGATGATAACGTTGTCATCATAGACTTGTTGGGGCCGAGTCTTGAAGACTTATTTGTATACTGTGGGAGGAAGTTTTCACTGAAAACAGTCTTAATGCTGGCCGAACAGATGATTACAAGAATAGAGTATGTGCATTCGAAAGGGTTTTTGCATAGAGACATCAAACCAGACAATTATCTCATGGGTCTTGGCAGGAAAGCAAATCAGGTTTACATCATTGACTTTGGACTTGCAAAAAGATATCGGGACCCAACTACAAATCGGCACATTCCATACAGAGAAAACAAAAGCTTAACAGGAACTGCACGATATGCTAGTTGCAATACCCATTTGGGAATCGATCAGAGTCGTCGGGATGATTTGGAGTCACTTGGCTATGTTCTTCTGTACTTCTTAAGAGGAAGCCTTCCTTGGCAGGGTCTAAAAGCTGCAACGAAGAAGCAAAAATATGATAAGATTTGTGAGAAGAAGTTATCAACTCCCATTGAGGTGCTATGTAAGGCGCATCCTGTGGAGTTTGCATCCTACTTCCACTATTGCCATTCCTTAACATTTGATCAACGTCCGGATTACTCATTCTTGAAGCGTCTGTTTCGTGACTTATTCACTCGCGAAGGATTTGAGTTTGATTATGTATTTGACTGGACAATCCTAAAGTACCAGCAGGCGCAACAGACAAGGGCTCATGGTCAATCAGCTCCAGCTGTAGCGACAAATGGTCAAGTAGTGACTAGCAGTCGAGCAGTGCCTATGGATGTGAGGAATCAGGGACTTGATGATGTTTCTAGACAAGCTCAGCCGCGAGAACATAAAAGATCAAGTCATCTTGTTCATATTCACAGGCCATTTAGATCATCTGCAGTCCAGAATTTGAGTGTTAATAACTCTATTGAAAAACATAATACGGGCAATGCTCTCAAAAGAAATTTACCAAAACCCGAGGGGCCAGCTGAAATTGCTAATGTAGGTCGCCAACACGGTGCTTCAAGCAGTTGGATGCCATCTCTAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04310 ko04340 ko04341 ko04390 ko04391 ko04392 ko04540 ko04710 ko04711 map04068 map04310 map04340 map04341 map04390 map04391 map04392 map04540 map04710 map04711
Pfam Domains
Protein Families
Pkinase

Protein Analysis

413

Amino Acids

46.86

Weight (kDa)

9.53

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 10 - 227 2.6e-27 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 10 - 269 2.3e-14 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 112 - 206 4.9e-08 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016276)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g35730 FvH4_2g35730 FvH4_2g35730
malus_domestica MD08G1115500.v1.1 MD15G1094700.v1.1
prunus_persica Prupe.1G449900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306051
rosa_laevigata RLG00000010828
rosa_roxburghii Rroxscaffold_7G00162090
rosa_rugosa Rorug06G0346600 Rorug06G0346700
rosa_samantha Rh6BG442100 Rh6CG473200 Rh6DG459500
rosa_wichuraiana Rw6G039990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 51
AccI GTMKAC 1 cut(s) 282
AccII CGCG 2 cut(s) 837, 1027
AccIII TCCGGA 1 cut(s) 790
AciI CCGC 5 cut(s) 51, 107, 189, 212, 1025
AclI AACGTT 1 cut(s) 234
AcoI YGGCCR 2 cut(s) 108, 324
AcsI RAATTY 2 cut(s) 1093, 1150
AfaI GTAC 2 cut(s) 614, 884
AfiI CCNNNNNNNGG 1 cut(s) 641
AflII CTTAAG 1 cut(s) 619
AgsI TTSAA 5 cut(s) 119, 269, 808, 1118, 1212
AhdI GACNNNNNGTC 2 cut(s) 942, 960
AjuI GAANNNNNNNTTGG 2 cut(s) 619, 651
AluBI AGCT 9 cut(s) 31, 145, 163, 516, 653, 923, 929, 1019, 1175
AluI AGCT 9 cut(s) 31, 145, 163, 516, 653, 923, 929, 1019, 1175
Alw26I GTCTC 3 cut(s) 83, 92, 375
AlwNI CAGNNNCTG 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 1163
Aor13HI TCCGGA 1 cut(s) 790
AoxI GGCC 6 cut(s) 108, 156, 257, 324, 1067, 1169
ApeKI GCWGC 1 cut(s) 653
ApoI RAATTY 2 cut(s) 1093, 1150
AspLEI GCGC 2 cut(s) 730, 895
AspS9I GGNCC 3 cut(s) 257, 472, 1169
AsuHPI GGTGA 1 cut(s) 74
AsuII TTCGAA 1 cut(s) 362
AvaI CYCGRG 1 cut(s) 1163
AvaII GGWCC 1 cut(s) 472
BanII GRGCYC 1 cut(s) 910
BbsI GAAGAC 1 cut(s) 276
BbvI GCAGC 1 cut(s) 640
BccI CCATC 1 cut(s) 1234
BclI TGATCA 2 cut(s) 220, 781
BcoDI GTCTC 3 cut(s) 83, 92, 375
BfaI CTAG 4 cut(s) 32, 540, 957, 1011
BfmI CTRYAG 3 cut(s) 176, 930, 1083
BfrI CTTAAG 1 cut(s) 619
BisI GCNGC 4 cut(s) 108, 190, 654, 1025
BlpI GCTNAGC 1 cut(s) 1020
BlsI GCNGC 4 cut(s) 109, 191, 655, 1026
Bme18I GGWCC 1 cut(s) 472
BmeRI GACNNNNNGTC 2 cut(s) 942, 960
BmeT110I CYCGRG 1 cut(s) 1163
BmgT120I GGNCC 3 cut(s) 257, 472, 1169
BmiI GGNNCC 4 cut(s) 258, 473, 474, 1170
BmsI GCATC 3 cut(s) 739, 755, 1212
BpiI GAAGAC 1 cut(s) 276
BpmI CTGGAG 2 cut(s) 114, 909
Bpu1102I GCTNAGC 1 cut(s) 1020
Bpu14I TTCGAA 1 cut(s) 362
Bsa29I ATCGAT 1 cut(s) 564
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 3 cut(s) 102, 635, 1164
BsaWI WCCGGW 1 cut(s) 790
Bsc4I CCNNNNNNNGG 1 cut(s) 641
Bse1I ACTGG 2 cut(s) 97, 872
Bse3DI GCAATG 2 cut(s) 192, 1141
BseAI TCCGGA 1 cut(s) 790
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 3 cut(s) 102, 635, 1164
BseGI GGATG 5 cut(s) 586, 730, 746, 985, 1227
BseLI CCNNNNNNNGG 1 cut(s) 641
BseMI GCAATG 2 cut(s) 192, 1141
BseMII CTCAG 1 cut(s) 1034
BseNI ACTGG 2 cut(s) 97, 872
BseXI GCAGC 1 cut(s) 640
BsgI GTGCAG 1 cut(s) 513
Bsh1236I CGCG 2 cut(s) 837, 1027
BshFI GGCC 6 cut(s) 110, 158, 259, 326, 1069, 1171
BshVI ATCGAT 1 cut(s) 564
BsiHKCI CYCGRG 1 cut(s) 1163
BsiSI CCGG 1 cut(s) 791
BslFI GGGAC 2 cut(s) 485, 1008
BslI CCNNNNNNNGG 1 cut(s) 641
BsmAI GTCTC 3 cut(s) 83, 92, 375
BsmFI GGGAC 2 cut(s) 485, 1008
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 6 cut(s) 110, 158, 259, 326, 1069, 1171
Bso31I GGTCTC 1 cut(s) 92
BsoBI CYCGRG 1 cut(s) 1163
Bsp119I TTCGAA 1 cut(s) 362
Bsp1286I GDGCHC 1 cut(s) 910
Bsp13I TCCGGA 1 cut(s) 790
Bsp143I GATC 5 cut(s) 220, 565, 781, 1040, 1076
Bsp1720I GCTNAGC 1 cut(s) 1020
Bsp68I TCGCGA 1 cut(s) 837
BspACI CCGC 5 cut(s) 51, 107, 189, 212, 1025
BspANI GGCC 6 cut(s) 110, 158, 259, 326, 1069, 1171
BspCNI CTCAG 1 cut(s) 1033
BspDI ATCGAT 1 cut(s) 564
BspEI TCCGGA 1 cut(s) 790
BspFNI CGCG 2 cut(s) 837, 1027
BspHI TCATGA 1 cut(s) 223
BspLI GGNNCC 4 cut(s) 258, 473, 474, 1170
BspMAI CTGCAG 1 cut(s) 1087
BspT104I TTCGAA 1 cut(s) 362
BspTI CTTAAG 1 cut(s) 619
BspTNI GGTCTC 1 cut(s) 92
BsrBI CCGCTC 1 cut(s) 51
BsrDI GCAATG 2 cut(s) 192, 1141
BsrI ACTGG 2 cut(s) 97, 872
BssECI CCNNGG 3 cut(s) 102, 635, 1164
BssMI GATC 5 cut(s) 220, 565, 781, 1040, 1076
BssNAI GTATAC 1 cut(s) 283
BssT1I CCWWGG 2 cut(s) 102, 635
Bst1107I GTATAC 1 cut(s) 283
Bst4CI ACNGT 3 cut(s) 287, 313, 1205
BstAFI CTTAAG 1 cut(s) 619
BstAPI GCANNNNNTGC 1 cut(s) 536
BstBI TTCGAA 1 cut(s) 362
BstC8I GCNNGC 4 cut(s) 199, 324, 891, 1173
BstDEI CTNAG 1 cut(s) 1020
BstF5I GGATG 5 cut(s) 586, 730, 746, 985, 1227
BstFNI CGCG 2 cut(s) 837, 1027
BstHHI GCGC 2 cut(s) 730, 895
BstKTI GATC 5 cut(s) 223, 568, 784, 1043, 1079
BstMAI GTCTC 3 cut(s) 83, 92, 375
BstMBI GATC 5 cut(s) 220, 565, 781, 1040, 1076
BstMWI GCNNNNNNNGC 4 cut(s) 142, 198, 536, 1181
BstSFI CTRYAG 3 cut(s) 176, 930, 1083
BstUI CGCG 2 cut(s) 837, 1027
BstV1I GCAGC 1 cut(s) 640
BstV2I GAAGAC 1 cut(s) 276
BstZ17I GTATAC 1 cut(s) 283
Bsu15I ATCGAT 1 cut(s) 564
BsuRI GGCC 6 cut(s) 110, 158, 259, 326, 1069, 1171
BsuTUI ATCGAT 1 cut(s) 564
BtsCI GGATG 5 cut(s) 586, 730, 746, 985, 1227
BtsI GCAGTG 1 cut(s) 975
BtsIMutI CAGTG 2 cut(s) 302, 975
BtuMI TCGCGA 1 cut(s) 837
Cac8I GCNNGC 4 cut(s) 199, 324, 891, 1173
CaiI CAGNNNCTG 1 cut(s) 527
CciI TCATGA 1 cut(s) 223
CfoI GCGC 2 cut(s) 730, 895
Cfr13I GGNCC 3 cut(s) 257, 472, 1169
ClaI ATCGAT 1 cut(s) 564
CseI GACGC 1 cut(s) 800
Csp6I GTAC 2 cut(s) 613, 883
CviAII CATG 4 cut(s) 202, 224, 406, 911
CviQI GTAC 2 cut(s) 613, 883
DdeI CTNAG 1 cut(s) 1020
DpnI GATC 5 cut(s) 222, 567, 783, 1042, 1078
DpnII GATC 5 cut(s) 220, 565, 781, 1040, 1076
DriI GACNNNNNGTC 2 cut(s) 942, 960
EaeI YGGCCR 2 cut(s) 108, 324
Eam1105I GACNNNNNGTC 2 cut(s) 942, 960
Eco130I CCWWGG 2 cut(s) 102, 635
Eco24I GRGCYC 1 cut(s) 910
Eco31I GGTCTC 1 cut(s) 92
Eco32I GATATC 1 cut(s) 467
Eco47I GGWCC 1 cut(s) 472
Eco88I CYCGRG 1 cut(s) 1163
EcoO109I RGGNCCY 1 cut(s) 472
EcoRV GATATC 1 cut(s) 467
EcoT14I CCWWGG 2 cut(s) 102, 635
EcoT38I GRGCYC 1 cut(s) 910
ErhI CCWWGG 2 cut(s) 102, 635
FaeI CATG 4 cut(s) 205, 227, 409, 914
FalI AAGNNNNNCTT 2 cut(s) 619, 651
FaqI GGGAC 2 cut(s) 485, 1008
FatI CATG 4 cut(s) 201, 223, 405, 910
FauI CCCGC 1 cut(s) 205
FauNDI CATATG 1 cut(s) 84
FbaI TGATCA 2 cut(s) 220, 781
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 4 cut(s) 108, 190, 654, 1025
FokI GGATG 5 cut(s) 593, 717, 733, 992, 1234
FriOI GRGCYC 1 cut(s) 910
Fsp4HI GCNGC 4 cut(s) 108, 190, 654, 1025
FspBI CTAG 4 cut(s) 32, 540, 957, 1011
GlaI GCGC 2 cut(s) 729, 894
GluI GCNGC 4 cut(s) 108, 190, 654, 1025
GsuI CTGGAG 2 cut(s) 114, 909
HaeIII GGCC 6 cut(s) 110, 158, 259, 326, 1069, 1171
HapII CCGG 1 cut(s) 791
HgaI GACGC 1 cut(s) 800
HhaI GCGC 2 cut(s) 730, 895
Hin1II CATG 4 cut(s) 205, 227, 409, 914
Hin6I GCGC 2 cut(s) 728, 893
HinP1I GCGC 2 cut(s) 728, 893
HindIII AAGCTT 1 cut(s) 514
HinfI GANTC 5 cut(s) 262, 561, 571, 590, 988
HpaII CCGG 1 cut(s) 791
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 2 cut(s) 283, 436
Hpy188I TCNGA 2 cut(s) 17, 570
Hpy8I GTNNAC 2 cut(s) 283, 436
Hpy99I CGWCG 1 cut(s) 579
HpyAV CCTTC 2 cut(s) 641, 833
HpyCH4III ACNGT 3 cut(s) 287, 313, 1205
HpyCH4IV ACGT 2 cut(s) 234, 787
HpyCH4V TGCA 9 cut(s) 197, 358, 376, 458, 530, 546, 656, 746, 1085
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 198, 536, 1181
HpyF3I CTNAG 1 cut(s) 1020
HpySE526I ACGT 2 cut(s) 234, 787
Hsp92II CATG 4 cut(s) 205, 227, 409, 914
HspAI GCGC 2 cut(s) 728, 893
KflI GGGWCCC 1 cut(s) 472
Kpn2I TCCGGA 1 cut(s) 790
Ksp22I TGATCA 2 cut(s) 220, 781
Kzo9I GATC 5 cut(s) 220, 565, 781, 1040, 1076
LmnI GCTCC 2 cut(s) 48, 928
Lsp1109I GCAGC 1 cut(s) 640
LweI GCATC 3 cut(s) 739, 755, 1212
MaeI CTAG 4 cut(s) 32, 540, 957, 1011
MaeII ACGT 2 cut(s) 234, 787
MaeIII GTNAC 4 cut(s) 122, 591, 821, 952
MalI GATC 5 cut(s) 222, 567, 783, 1042, 1078
MbiI CCGCTC 1 cut(s) 51
MboI GATC 5 cut(s) 220, 565, 781, 1040, 1076
MboII GAAGA 4 cut(s) 281, 599, 673, 703
MhlI GDGCHC 1 cut(s) 910
MluCI AATT 6 cut(s) 42, 397, 1093, 1150, 1179, 1234
MlyI GAGTC 3 cut(s) 271, 580, 599
MmeI TCCRAC 1 cut(s) 1199
MnlI CCTC 9 cut(s) 11, 83, 148, 176, 285, 617, 706, 978, 1159
MroI TCCGGA 1 cut(s) 790
MseI TTAA 6 cut(s) 27, 317, 518, 620, 773, 1104
MslI CAYNNNNRTG 1 cut(s) 713
MspA1I CMGCKG 2 cut(s) 929, 1175
MspCI CTTAAG 1 cut(s) 619
MspI CCGG 1 cut(s) 791
Mva1269I GAATGC 1 cut(s) 358
MvnI CGCG 2 cut(s) 837, 1027
MwoI GCNNNNNNNGC 4 cut(s) 142, 198, 536, 1181
NdeI CATATG 1 cut(s) 84
NdeII GATC 5 cut(s) 220, 565, 781, 1040, 1076
NlaIII CATG 4 cut(s) 205, 227, 409, 914
NlaIV GGNNCC 4 cut(s) 258, 473, 474, 1170
NmeAIII GCCGAG 1 cut(s) 285
NmuCI GTSAC 3 cut(s) 591, 821, 952
NruI TCGCGA 1 cut(s) 837
NspV TTCGAA 1 cut(s) 362
PagI TCATGA 1 cut(s) 223
PctI GAATGC 1 cut(s) 358
PfeI GAWTC 2 cut(s) 561, 988
PkrI GCNGC 4 cut(s) 109, 191, 655, 1026
PleI GAGTC 3 cut(s) 270, 579, 598
PpsI GAGTC 3 cut(s) 270, 579, 598
PpuMI RGGWCCY 1 cut(s) 472
Psp1406I AACGTT 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 472
PspN4I GGNNCC 4 cut(s) 258, 473, 474, 1170
PspPI GGNCC 3 cut(s) 257, 472, 1169
PspPPI RGGWCCY 1 cut(s) 472
PstI CTGCAG 1 cut(s) 1087
PstNI CAGNNNCTG 1 cut(s) 527
PvuII CAGCTG 2 cut(s) 929, 1175
RruI TCGCGA 1 cut(s) 837
RsaI GTAC 2 cut(s) 614, 884
RsaNI GTAC 2 cut(s) 613, 883
RseI CAYNNNNRTG 1 cut(s) 713
SaqAI TTAA 6 cut(s) 27, 317, 518, 620, 773, 1104
SatI GCNGC 4 cut(s) 108, 190, 654, 1025
Sau3AI GATC 5 cut(s) 220, 565, 781, 1040, 1076
Sau96I GGNCC 3 cut(s) 257, 472, 1169
SchI GAGTC 3 cut(s) 271, 580, 599
SduI GDGCHC 1 cut(s) 910
SfaNI GCATC 3 cut(s) 739, 755, 1212
SfcI CTRYAG 3 cut(s) 176, 930, 1083
SfuI TTCGAA 1 cut(s) 362
SinI GGWCC 1 cut(s) 472
SmiMI CAYNNNNRTG 1 cut(s) 713
SmlI CTYRAG 1 cut(s) 619
SmoI CTYRAG 1 cut(s) 619
Sse9I AATT 6 cut(s) 42, 397, 1093, 1150, 1179, 1234
SsiI CCGC 5 cut(s) 51, 107, 189, 212, 1025
SspMI CTAG 4 cut(s) 32, 540, 957, 1011
StyI CCWWGG 2 cut(s) 102, 635
TaaI ACNGT 3 cut(s) 287, 313, 1205
TaiI ACGT 2 cut(s) 237, 790
TaqI TCGA 3 cut(s) 362, 564, 964
TasI AATT 6 cut(s) 42, 397, 1093, 1150, 1179, 1234
TatI WGTACW 1 cut(s) 612
TauI GCSGC 3 cut(s) 110, 192, 1027
TfiI GAWTC 2 cut(s) 561, 988
Tru1I TTAA 6 cut(s) 27, 317, 518, 620, 773, 1104
Tru9I TTAA 6 cut(s) 27, 317, 518, 620, 773, 1104
TscAI CASTG 2 cut(s) 309, 975
TseFI GTSAC 3 cut(s) 591, 821, 952
TseI GCWGC 1 cut(s) 653
Tsp45I GTSAC 3 cut(s) 591, 821, 952
TspDTI ATGAA 2 cut(s) 218, 1046
TspRI CASTG 2 cut(s) 309, 975
Vha464I CTTAAG 1 cut(s) 619
VpaK11BI GGWCC 1 cut(s) 472
XapI RAATTY 2 cut(s) 1093, 1150
XbaI TCTAGA 1 cut(s) 1010
XmiI GTMKAC 1 cut(s) 282
XspI CTAG 4 cut(s) 32, 540, 957, 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.