MD08G1115900.v1.1

ER membrane protein complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011093
Physical Location & Seq
Forward (+)
10497766 .. 10503766
6001 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1115900.v1.1.491

Sequence Viewer

Length: 2958 bp
ATGGGCATGGCGGTTCGAGGTTTCGTGCTTCTTCTTCTGTTCTTATCAGCTGCAAATCTCAGTCTTTCGCTCTACGAAGATCAAGTCGGTCTCATGGATTGGCACCAGCAGTACATAGGGAAGGTGAAGGAAGCAGTGTTCCATACTCCGAAAAGCGGGAGGAGGCGGGTTGTGGTGTCCACTGAGGAGAATGTTATAGCATCGCTTGATCTTCGGCATGGGGGGATAATTTGGAGACGTGTTCTTGGGAGCAATGATGCTATAGATGGAATCGACATAGCATTGGGAAAATATGTCGTTACCCTTTCGTCAGACGGAAGTATTTTAAGAGCATGGAACCTTCCTGATGGTCAGATGGTTTGGGAATCTTTTCTAGAGGGCTCAATGTCCTCTAAATCTTTACTAAGTGTCCCGACAAGTTTGAAAGTTGACAAGGGAAATTTGATCCTTGTTTTTGGAAAAGGGTCTCTACATGCCATTTCTAGCATTGATGGTGAGGTTCTTTGGGAAAAGGATTTTGCAGCTGAAAGTGTAGAGGTTCAACAGATTATTCAGCCTCTGGGCGGTGATGTGGCTTATGTACTCGGATTTGTTGGTTCTTCCCAGTTTGATGCATATCAAATGAATGTTAGGAACGGAGAGTTGCTGAAGCACAGCAGTGCACCCTTTTCGGGTGGCTTTTCAGGGGAAGCACTGCTTGTTTCTAGTGAAATTCTTGTGACATTGGATTCCACGAGGTCAAAGCTGGTAATTGTAAGTTTTCAGGATGGAGAAATTAGCTATCAACAGTCACCCATATCCGATATTTTTGGGGATTCTTTTGGAACACCAGTGCTGTTACCTTCAAAACTTCCAGGATTGTTTTCTGTAAAACTTAATGGGGCTGTCATATTCATAAGAGTGACTGGTGAAGGAAAGTTGGAGGTGCTGGATAAAGTAAATGATGTGGCGGCTATCAGTGATGCAATCTCACTTTCAGACGGGCAACAGGCTTTTGGACTAGTCCAACATGGAGATGGTAAGATCCATCTTACAGTAAAGCCTACTCATGACTCAAGCACTGATCTGCTCAAAGAAAGTATTGTTGTGGACAGTCAGAGTGGGGTTGTTCATAAGGTTTTCATAAACAATTATATCCGGACAGACAGGTCTAATGGGTTTAGGGCCTTAATCGTCATGGAGGATGATTCACTTTTGTTGTTACAACAAGGTGCAGTTGTATGGAGTAGGGAGGATGGGCTTGCCTCAATTATTGATGTAGTGACGTCAGAACTACCTGTGGAAAAGGTAGGCGTATCAGTTGCGAAAGTGGAGCAGAACCTCTTCGATTGGCTCAAGGGACACATACTGAAGCTTAAGGGAACCTTAATGCTTGCAACTGCTGCGGATGTTGCCGCTATACAAGAGATGAGGCTAAAAAGTTTTGAAAAGAGCAAACTGACACGTGACCATAATGGTTTTCGGAAGCTCCTCATAGTACTGACGAGAGCAGGAAAACTTTTTGCCTTGCACACCGGATATGGGCAAGTTGTCTGGTCTCTCTTACTGCCTACTCTGCGTAAATCTGAAACGTGCGAGTACCCGACTGGGTTGAAAATTTATCAGTGGCAGGTACCCCATCATCATGCACTGGATGAAAATCCATCTGTCCTCATAGTAGGTCGATGCGGGCAAAGCTCTGAGGCACCAGGTGTTCTTTCTATTGTTGACGCATACACGGGCAAGGAAATTAATTCAATGGCTCCAGCACATTCTATTGTGCAAGTTATACCACTGCCATTTACTGATTCGACAGAACAGCGTCTTCATCTACTAATCGATGCTAACCAGCGTGGGCATTTATACCCAAGAACATCTGATGCTATTGATATTTTTCAAAGGGAGTTCACAAACATTTACTGGTACTCAGTTGATGCTGATAATGACATTATCAAAGGACATGCTTTGAAGGGCACTTGCAACCAGGAAGCTGTAGATGACTACTGCTTTGAGTCCAAGGATATATGGTCGATTGTATTCCCATCTGACTCAGAAAAAATCATTGCAACTGTGACAAGAAAATTGAGTGAGGTGGTTCATACTCAAGCAAAGGTTATTGCAGACTCAGAGGTGATGTATAAATACATATCTAAAAATCTACTCTTTGTGGCAACTGTTGCACCAAAAGGCAGTGGTGAAATTGGAACAGCTACCCCAGAGGAGTCCTGGTTGACTGTATATCTTATTGATACTGTAACTGGTCGTATATTGCACCGGATGACCCATCACGGTTCACAGGGGCCTGTCCATGCTGTTTTCAGTGAGAATTGGGTTGTTTACCACTACTTCAATCTTAGAGCACACAGATACGAAATGTCAGTCATTGAGATATATGATCAGTCCCGAGCGGATAATAAGGATGTTTTGAAGCTTGTTCTTGGGAAGCATAATCTTACTTCACCTATTTCATCATATTCTCGACCAGAGGTTGTGACGAAATCACAGTCTTACTATTTCACGTATTCTGTTAAAGCAATAGCTGTGACATTAACAGCAAAGGGTATAACATCAAAGCAGCTTCTCATTGGTACAATCAATGATCAGGTTTTGGCACTTGACAAGCGTTATCTGGATCCCCGTCGGTCACTCAATCCCACACAAGCGGAGAAAGAGGAAGGCATTATTCCCCTAACTGATACATTGCCAATAATTCCTCAGTCATATGTGACACATAACCTCAAAGTGGAAGGACTTCGAGGCATCGTGACAGTACCTGCCAAGCTGGAATCAACAACCCTTGCCTTTACGTACGGAGTGGATCTGTTTTTCACTCAACTGGCTCCCTCGAGGACCTATGATTCGCTAACTGATGATTTCAGCTACGCGTTGCTTCTCATCACCATAGTTGCACTCATAGCAGCAATCTTTGTAACATGGGTCTGGTCGGAGAAGAAAGAACTAAAAGAGGAGTGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1620 PQQ_2

Protein Analysis

986

Amino Acids

108.9

Weight (kDa)

5.89

Isoelectric Point (pI)

31.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EMC1_N PF25293 24 - 412 1.6e-134 EMC1 N-terminal beta-propeller domain
PQQ_2 PF13360 48 - 262 5.9e-13 Outer membrane protein assembly factor BamB
Beta-prop_ACSF4 PF13570 54 - 280 4.4e-07 Beta-alanine-activating enzyme, beta-propeller
EMC1_C PF07774 767 - 984 4.5e-68 ER membrane protein complex subunit 1, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11560
fragaria_vesca FvH4_2g35850
malus_domestica MD08G1115900.v1.1 MD15G1095600.v1.1
prunus_persica Prupe.1G450600_v2.0.a1 Prupe.1G450600_v2.0.a1
pyrus_communis pycom08g09670 pycom08g09680 pycom15g08790
rosa_chinensis RchiOBHm_Chr6g0305971
rosa_laevigata RLG00000010841
rosa_multiflora Rmu_co8311943.1_g000001 Rmu_sc0001809.1_g000056
rosa_roxburghii Rroxscaffold_7G00162180
rosa_rugosa Rorug06G0345700
rosa_samantha Rh6AG457600 Rh6BG443500 Rh6CG471700 Rh6DG458100
rosa_wichuraiana Rw6G039920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1147
AatII GACGTC 1 cut(s) 1268
AbsI CCTCGAGG 1 cut(s) 2824
Acc36I ACCTGC 2 cut(s) 1600, 2761
Acc65I GGTACC 1 cut(s) 1612
AccB1I GGYRCC 3 cut(s) 102, 1612, 1684
AccBSI CCGCTC 1 cut(s) 2387
AccII CGCG 1 cut(s) 2864
AccIII TCCGGA 1 cut(s) 1137
AclWI GGATC 5 cut(s) 439, 1018, 2606, 2619, 2805
AcsI RAATTY 3 cut(s) 439, 711, 1596
AcuI CTGAAG 2 cut(s) 668, 1370
AcvI CACGTG 1 cut(s) 1445
AcyI GRCGYC 1 cut(s) 1265
AdeI CACNNNGTG 1 cut(s) 1691
AfaI GTAC 9 cut(s) 113, 582, 1479, 1580, 1614, 1904, 2569, 2751, 2789
AfiI CCNNNNNNNGG 5 cut(s) 155, 563, 671, 1521, 2722
AflII CTTAAG 1 cut(s) 1355
AflIII ACRYGT 3 cut(s) 238, 1442, 2862
AhlI ACTAGT 1 cut(s) 1000
AjiI CACGTC 1 cut(s) 239
AjnI CCWGG 4 cut(s) 853, 1687, 1962, 2204
AleI CACNNNNGTG 1 cut(s) 657
AloI GAACNNNNNNTCC 2 cut(s) 122, 154
Alw21I GWGCWC 2 cut(s) 664, 2341
Alw26I GTCTC 4 cut(s) 95, 229, 471, 1542
Alw44I GTGCAC 1 cut(s) 660
AlwI GGATC 5 cut(s) 439, 1018, 2606, 2619, 2805
AlwNI CAGNNNCTG 2 cut(s) 559, 2753
Ama87I CYCGRG 2 cut(s) 2382, 2824
Aor13HI TCCGGA 1 cut(s) 1137
AoxI GGCC 2 cut(s) 1164, 2279
ApaLI GTGCAC 1 cut(s) 660
ApeKI GCWGC 5 cut(s) 50, 521, 1382, 2554, 2897
ApoI RAATTY 3 cut(s) 439, 711, 1596
AseI ATTAAT 1 cut(s) 1731
Asp700I GAANNNNTTC 3 cut(s) 369, 1322, 2730
Asp718I GGTACC 1 cut(s) 1612
AspS9I GGNCC 3 cut(s) 1164, 2279, 2829
AsuHPI GGTGA 9 cut(s) 136, 506, 578, 783, 920, 2122, 2186, 2430, 2869
AvaI CYCGRG 2 cut(s) 2382, 2824
AvaII GGWCC 1 cut(s) 2829
BaeGI GKGCMC 2 cut(s) 664, 1955
BamHI GGATCC 1 cut(s) 2611
BanI GGYRCC 3 cut(s) 102, 1612, 1684
BanII GRGCYC 1 cut(s) 383
BauI CACGAG 1 cut(s) 733
BbrPI CACGTG 1 cut(s) 1445
BbsI GAAGAC 1 cut(s) 1796
Bbv12I GWGCWC 2 cut(s) 664, 2341
BbvI GCAGC 5 cut(s) 37, 533, 1369, 2566, 2909
BcgI CGANNNNNNTGC 2 cut(s) 651, 685
BciT130I CCWGG 4 cut(s) 855, 1689, 1964, 2206
BclI TGATCA 2 cut(s) 2374, 2578
BcoDI GTCTC 4 cut(s) 95, 229, 471, 1542
BcuI ACTAGT 1 cut(s) 1000
BfaI CTAG 4 cut(s) 374, 483, 705, 1001
BfmI CTRYAG 2 cut(s) 261, 1971
BfrI CTTAAG 1 cut(s) 1355
BfuAI ACCTGC 2 cut(s) 1600, 2761
BisI GCNGC 7 cut(s) 51, 522, 951, 1383, 1395, 2555, 2898
BlsI GCNGC 7 cut(s) 52, 523, 952, 1384, 1396, 2556, 2899
BmcAI AGTACT 1 cut(s) 1479
Bme1390I CCNGG 4 cut(s) 855, 1689, 1964, 2206
Bme18I GGWCC 1 cut(s) 2829
BmeT110I CYCGRG 2 cut(s) 2382, 2824
BmgBI CACGTC 1 cut(s) 239
BmgT120I GGNCC 3 cut(s) 1164, 2279, 2829
BmiI GGNNCC 9 cut(s) 104, 338, 1363, 1614, 1686, 1743, 2280, 2613, 2820
BmrFI CCNGG 4 cut(s) 855, 1689, 1964, 2206
BmrI ACTGGG 2 cut(s) 598, 1596
BmsI GCATC 9 cut(s) 209, 247, 601, 952, 1655, 1810, 1849, 1903, 2748
BmuI ACTGGG 2 cut(s) 598, 1596
BpiI GAAGAC 1 cut(s) 1796
BpmI CTGGAG 1 cut(s) 1728
BpuEI CTTGAG 3 cut(s) 1039, 1319, 2067
Bsa29I ATCGAT 1 cut(s) 1818
BsaAI YACGTR 3 cut(s) 1445, 2499, 2787
BsaBI GATNNNNATC 2 cut(s) 615, 1638
BsaHI GRCGYC 1 cut(s) 1265
BsaI GGTCTC 3 cut(s) 95, 471, 1542
BsaJI CCNNGG 1 cut(s) 1995
BsaWI WCCGGW 3 cut(s) 1137, 1514, 2253
BsaXI ACNNNNNCTCC 3 cut(s) 2192, 2222, 2939
Bsc4I CCNNNNNNNGG 5 cut(s) 155, 563, 671, 1521, 2722
Bse1I ACTGG 8 cut(s) 604, 830, 910, 1591, 1635, 1904, 2242, 2820
Bse3DI GCAATG 3 cut(s) 259, 2040, 2678
Bse8I GATNNNNATC 2 cut(s) 615, 1638
BseAI TCCGGA 1 cut(s) 1137
BseBI CCWGG 4 cut(s) 855, 1689, 1964, 2206
BseCI ATCGAT 1 cut(s) 1818
BseDI CCNNGG 1 cut(s) 1995
BseGI GGATG 7 cut(s) 772, 1189, 1240, 1393, 1639, 2262, 2404
BseJI GATNNNNATC 2 cut(s) 615, 1638
BseLI CCNNNNNNNGG 5 cut(s) 155, 563, 671, 1521, 2722
BseMI GCAATG 3 cut(s) 259, 2040, 2678
BseMII CTCAG 7 cut(s) 73, 174, 1671, 1920, 2043, 2118, 2708
BseNI ACTGG 8 cut(s) 604, 830, 910, 1591, 1635, 1904, 2242, 2820
BseRI GAGGAG 4 cut(s) 175, 200, 1460, 2213
BseSI GKGCMC 2 cut(s) 664, 1955
BseXI GCAGC 5 cut(s) 37, 533, 1369, 2566, 2909
BsgI GTGCAG 1 cut(s) 1233
Bsh1236I CGCG 1 cut(s) 2864
BshFI GGCC 2 cut(s) 1166, 2281
BshNI GGYRCC 3 cut(s) 102, 1612, 1684
BshVI ATCGAT 1 cut(s) 1818
BsiHKAI GWGCWC 2 cut(s) 664, 2341
BsiHKCI CYCGRG 2 cut(s) 2382, 2824
BsiSI CCGG 3 cut(s) 1138, 1515, 2254
BsiWI CGTACG 1 cut(s) 2787
BslFI GGGAC 3 cut(s) 395, 1353, 2365
BslI CCNNNNNNNGG 5 cut(s) 155, 563, 671, 1521, 2722
BsmAI GTCTC 4 cut(s) 95, 229, 471, 1542
BsmBI CGTCTC 1 cut(s) 229
BsmFI GGGAC 3 cut(s) 395, 1353, 2365
BsnI GGCC 2 cut(s) 1166, 2281
Bso31I GGTCTC 3 cut(s) 95, 471, 1542
BsoBI CYCGRG 2 cut(s) 2382, 2824
Bsp1286I GDGCHC 4 cut(s) 383, 664, 1955, 2341
Bsp13I TCCGGA 1 cut(s) 1137
Bsp143I GATC 9 cut(s) 79, 208, 444, 1023, 1063, 2374, 2578, 2611, 2797
BspANI GGCC 2 cut(s) 1166, 2281
BspCNI CTCAG 7 cut(s) 72, 175, 1672, 1919, 2042, 2117, 2707
BspDI ATCGAT 1 cut(s) 1818
BspEI TCCGGA 1 cut(s) 1137
BspFNI CGCG 1 cut(s) 2864
BspHI TCATGA 1 cut(s) 1048
BspLI GGNNCC 9 cut(s) 104, 338, 1363, 1614, 1686, 1743, 2280, 2613, 2820
BspMI ACCTGC 2 cut(s) 1600, 2761
BspPI GGATC 5 cut(s) 439, 1018, 2606, 2619, 2805
BspT107I GGYRCC 3 cut(s) 102, 1612, 1684
BspTI CTTAAG 1 cut(s) 1355
BspTNI GGTCTC 3 cut(s) 95, 471, 1542
BsrBI CCGCTC 1 cut(s) 2387
BsrDI GCAATG 3 cut(s) 259, 2040, 2678
BsrI ACTGG 8 cut(s) 604, 830, 910, 1591, 1635, 1904, 2242, 2820
BssECI CCNNGG 1 cut(s) 1995
BssMI GATC 9 cut(s) 79, 208, 444, 1023, 1063, 2374, 2578, 2611, 2797
BssNI GRCGYC 1 cut(s) 1265
BssSI CACGAG 1 cut(s) 733
BssT1I CCWWGG 1 cut(s) 1995
Bst2BI CACGAG 1 cut(s) 733
Bst2UI CCWGG 4 cut(s) 855, 1689, 1964, 2206
Bst6I CTCTTC 1 cut(s) 1328
BstACI GRCGYC 1 cut(s) 1265
BstAFI CTTAAG 1 cut(s) 1355
BstAPI GCANNNNNTGC 2 cut(s) 1382, 2156
BstBAI YACGTR 3 cut(s) 1445, 2499, 2787
BstC8I GCNNGC 3 cut(s) 1242, 1374, 1670
BstDEI CTNAG 9 cut(s) 59, 183, 404, 1680, 1906, 2029, 2104, 2333, 2694
BstF5I GGATG 7 cut(s) 772, 1189, 1240, 1393, 1639, 2262, 2404
BstFNI CGCG 1 cut(s) 2864
BstKTI GATC 9 cut(s) 82, 211, 447, 1026, 1066, 2377, 2581, 2614, 2800
BstMAI GTCTC 4 cut(s) 95, 229, 471, 1542
BstMBI GATC 9 cut(s) 79, 208, 444, 1023, 1063, 2374, 2578, 2611, 2797
BstMWI GCNNNNNNNGC 6 cut(s) 1382, 1391, 1555, 1674, 2156, 2894
BstNI CCWGG 4 cut(s) 855, 1689, 1964, 2206
BstNSI RCATGY 2 cut(s) 476, 1943
BstSCI CCNGG 4 cut(s) 853, 1687, 1962, 2204
BstSFI CTRYAG 2 cut(s) 261, 1971
BstSLI GKGCMC 2 cut(s) 664, 1955
BstSNI TACGTA 1 cut(s) 2787
BstUI CGCG 1 cut(s) 2864
BstV1I GCAGC 5 cut(s) 37, 533, 1369, 2566, 2909
BstV2I GAAGAC 1 cut(s) 1796
BstX2I RGATCY 3 cut(s) 1023, 2611, 2797
BstYI RGATCY 3 cut(s) 1023, 2611, 2797
Bsu15I ATCGAT 1 cut(s) 1818
BsuRI GGCC 2 cut(s) 1166, 2281
BsuTUI ATCGAT 1 cut(s) 1818
BtgZI GCGATG 1 cut(s) 186
BtrI CACGTC 1 cut(s) 239
BtsCI GGATG 7 cut(s) 772, 1189, 1240, 1393, 1639, 2262, 2404
BtsI GCAGTG 5 cut(s) 141, 664, 692, 1772, 2176
BveI ACCTGC 2 cut(s) 1600, 2761
Cac8I GCNNGC 3 cut(s) 1242, 1374, 1670
CaiI CAGNNNCTG 2 cut(s) 559, 2753
CciI TCATGA 1 cut(s) 1048
Cfr13I GGNCC 3 cut(s) 1164, 2279, 2829
ClaI ATCGAT 1 cut(s) 1818
CseI GACGC 2 cut(s) 1718, 1790
CsiI ACCWGGT 1 cut(s) 1687
Csp6I GTAC 9 cut(s) 112, 581, 1478, 1579, 1613, 1903, 2568, 2750, 2788
CviQI GTAC 9 cut(s) 112, 581, 1478, 1579, 1613, 1903, 2568, 2750, 2788
DdeI CTNAG 9 cut(s) 59, 183, 404, 1680, 1906, 2029, 2104, 2333, 2694
DpnI GATC 9 cut(s) 81, 210, 446, 1025, 1065, 2376, 2580, 2613, 2799
DpnII GATC 9 cut(s) 79, 208, 444, 1023, 1063, 2374, 2578, 2611, 2797
DraIII CACNNNGTG 1 cut(s) 1691
DrdI GACNNNNNNGTC 1 cut(s) 1147
DseDI GACNNNNNNGTC 1 cut(s) 1147
Eam1104I CTCTTC 1 cut(s) 1328
EarI CTCTTC 1 cut(s) 1328
Eco105I TACGTA 1 cut(s) 2787
Eco130I CCWWGG 1 cut(s) 1995
Eco24I GRGCYC 1 cut(s) 383
Eco31I GGTCTC 3 cut(s) 95, 471, 1542
Eco47I GGWCC 1 cut(s) 2829
Eco57I CTGAAG 2 cut(s) 668, 1370
Eco72I CACGTG 1 cut(s) 1445
Eco88I CYCGRG 2 cut(s) 2382, 2824
EcoO109I RGGNCCY 3 cut(s) 1164, 2279, 2829
EcoRII CCWGG 4 cut(s) 853, 1687, 1962, 2204
EcoT14I CCWWGG 1 cut(s) 1995
EcoT22I ATGCAT 1 cut(s) 616
EcoT38I GRGCYC 1 cut(s) 383
ErhI CCWWGG 1 cut(s) 1995
Esp3I CGTCTC 1 cut(s) 229
FalI AAGNNNNNCTT 4 cut(s) 681, 713, 1349, 1381
FaqI GGGAC 3 cut(s) 395, 1353, 2365
FauI CCCGC 3 cut(s) 149, 159, 1661
FauNDI CATATG 1 cut(s) 2701
FbaI TGATCA 2 cut(s) 2374, 2578
Fnu4HI GCNGC 7 cut(s) 51, 522, 951, 1383, 1395, 2555, 2898
FokI GGATG 7 cut(s) 779, 1196, 1247, 1400, 1646, 2269, 2411
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 7 cut(s) 51, 522, 951, 1383, 1395, 2555, 2898
FspBI CTAG 4 cut(s) 374, 483, 705, 1001
GluI GCNGC 7 cut(s) 51, 522, 951, 1383, 1395, 2555, 2898
GsuI CTGGAG 1 cut(s) 1728
HaeIII GGCC 2 cut(s) 1166, 2281
HapII CCGG 3 cut(s) 1138, 1515, 2254
HgaI GACGC 2 cut(s) 1718, 1790
Hin1I GRCGYC 1 cut(s) 1265
HincII GTYRAC 3 cut(s) 430, 1708, 2211
HindII GTYRAC 3 cut(s) 430, 1708, 2211
HindIII AAGCTT 2 cut(s) 1352, 2408
HpaII CCGG 3 cut(s) 1138, 1515, 2254
HphI GGTGA 9 cut(s) 136, 506, 578, 783, 920, 2122, 2186, 2430, 2869
Hpy166II GTNNAC 9 cut(s) 180, 430, 662, 1090, 1708, 1887, 2211, 2273, 2317
Hpy8I GTNNAC 9 cut(s) 180, 430, 662, 1090, 1708, 1887, 2211, 2273, 2317
Hpy99I CGWCG 1 cut(s) 2622
HpyAV CCTTC 8 cut(s) 115, 121, 350, 852, 905, 1942, 2648, 2720
HpyCH4IV ACGT 6 cut(s) 238, 1265, 1444, 1571, 2498, 2786
HpyF10VI GCNNNNNNNGC 6 cut(s) 1382, 1391, 1555, 1674, 2156, 2894
HpyF3I CTNAG 9 cut(s) 59, 183, 404, 1680, 1906, 2029, 2104, 2333, 2694
HpySE526I ACGT 6 cut(s) 238, 1265, 1444, 1571, 2498, 2786
Hsp92I GRCGYC 1 cut(s) 1265
Kpn2I TCCGGA 1 cut(s) 1137
KpnI GGTACC 1 cut(s) 1616
Ksp22I TGATCA 2 cut(s) 2374, 2578
Kzo9I GATC 9 cut(s) 79, 208, 444, 1023, 1063, 2374, 2578, 2611, 2797
LmnI GCTCC 5 cut(s) 249, 1312, 1473, 1747, 2824
Lsp1109I GCAGC 5 cut(s) 37, 533, 1369, 2566, 2909
LweI GCATC 9 cut(s) 209, 247, 601, 952, 1655, 1810, 1849, 1903, 2748
MabI ACCWGGT 1 cut(s) 1687
MaeI CTAG 4 cut(s) 374, 483, 705, 1001
MaeII ACGT 6 cut(s) 238, 1265, 1444, 1571, 2498, 2786
MalI GATC 9 cut(s) 81, 210, 446, 1025, 1065, 2376, 2580, 2613, 2799
MbiI CCGCTC 1 cut(s) 2387
MboI GATC 9 cut(s) 79, 208, 444, 1023, 1063, 2374, 2578, 2611, 2797
MboII GAAGA 8 cut(s) 23, 26, 89, 203, 591, 1315, 1796, 2941
MflI RGATCY 3 cut(s) 1023, 2611, 2797
MhlI GDGCHC 4 cut(s) 383, 664, 1955, 2341
MluI ACGCGT 1 cut(s) 2862
MlyI GAGTC 5 cut(s) 1046, 2000, 2021, 2096, 2210
MmeI TCCRAC 3 cut(s) 900, 1030, 2904
Mph1103I ATGCAT 1 cut(s) 616
MroI TCCGGA 1 cut(s) 1137
MroXI GAANNNNTTC 3 cut(s) 369, 1322, 2730
MseI TTAA 8 cut(s) 326, 876, 1169, 1356, 1367, 1731, 2508, 2528
MslI CAYNNNNRTG 4 cut(s) 657, 899, 1014, 1623
MspA1I CMGCKG 2 cut(s) 50, 524
MspCI CTTAAG 1 cut(s) 1355
MspI CCGG 3 cut(s) 1138, 1515, 2254
MspR9I CCNGG 4 cut(s) 855, 1689, 1964, 2206
MvaI CCWGG 4 cut(s) 855, 1689, 1964, 2206
MvnI CGCG 1 cut(s) 2864
MwoI GCNNNNNNNGC 6 cut(s) 1382, 1391, 1555, 1674, 2156, 2894
NdeI CATATG 1 cut(s) 2701
NdeII GATC 9 cut(s) 79, 208, 444, 1023, 1063, 2374, 2578, 2611, 2797
NlaIV GGNNCC 9 cut(s) 104, 338, 1363, 1614, 1686, 1743, 2280, 2613, 2820
NsiI ATGCAT 1 cut(s) 616
NspI RCATGY 2 cut(s) 476, 1943
OliI CACNNNNGTG 1 cut(s) 657
PaeR7I CTCGAG 1 cut(s) 2824
PagI TCATGA 1 cut(s) 1048
PdmI GAANNNNTTC 3 cut(s) 369, 1322, 2730
PfeI GAWTC 8 cut(s) 270, 365, 728, 815, 1187, 1787, 2765, 2837
Pfl23II CGTACG 1 cut(s) 2787
PfoI TCCNGGA 1 cut(s) 853
PkrI GCNGC 7 cut(s) 52, 523, 952, 1384, 1396, 2556, 2899
PleI GAGTC 5 cut(s) 1046, 1999, 2021, 2096, 2209
PmaCI CACGTG 1 cut(s) 1445
PmlI CACGTG 1 cut(s) 1445
PpsI GAGTC 5 cut(s) 1046, 1999, 2021, 2096, 2209
Ppu21I YACGTR 3 cut(s) 1445, 2499, 2787
PpuMI RGGWCCY 1 cut(s) 2829
PshBI ATTAAT 1 cut(s) 1731
Psp5II RGGWCCY 1 cut(s) 2829
Psp6I CCWGG 4 cut(s) 853, 1687, 1962, 2204
PspCI CACGTG 1 cut(s) 1445
PspGI CCWGG 4 cut(s) 853, 1687, 1962, 2204
PspLI CGTACG 1 cut(s) 2787
PspN4I GGNNCC 9 cut(s) 104, 338, 1363, 1614, 1686, 1743, 2280, 2613, 2820
PspPI GGNCC 3 cut(s) 1164, 2279, 2829
PspPPI RGGWCCY 1 cut(s) 2829
PspXI VCTCGAGB 1 cut(s) 2824
PstNI CAGNNNCTG 2 cut(s) 559, 2753
PsuI RGATCY 3 cut(s) 1023, 2611, 2797
PvuII CAGCTG 2 cut(s) 50, 524
RsaI GTAC 9 cut(s) 113, 582, 1479, 1580, 1614, 1904, 2569, 2751, 2789
RsaNI GTAC 9 cut(s) 112, 581, 1478, 1579, 1613, 1903, 2568, 2750, 2788
RseI CAYNNNNRTG 4 cut(s) 657, 899, 1014, 1623
SaqAI TTAA 8 cut(s) 326, 876, 1169, 1356, 1367, 1731, 2508, 2528
SatI GCNGC 7 cut(s) 51, 522, 951, 1383, 1395, 2555, 2898
Sau3AI GATC 9 cut(s) 79, 208, 444, 1023, 1063, 2374, 2578, 2611, 2797
Sau96I GGNCC 3 cut(s) 1164, 2279, 2829
ScaI AGTACT 1 cut(s) 1479
SchI GAGTC 5 cut(s) 1046, 2000, 2021, 2096, 2210
ScrFI CCNGG 4 cut(s) 855, 1689, 1964, 2206
SduI GDGCHC 4 cut(s) 383, 664, 1955, 2341
SexAI ACCWGGT 1 cut(s) 1687
SfaNI GCATC 9 cut(s) 209, 247, 601, 952, 1655, 1810, 1849, 1903, 2748
SfcI CTRYAG 2 cut(s) 261, 1971
Sfr274I CTCGAG 1 cut(s) 2824
SinI GGWCC 1 cut(s) 2829
SlaI CTCGAG 1 cut(s) 2824
SmiMI CAYNNNNRTG 4 cut(s) 657, 899, 1014, 1623
SmlI CTYRAG 5 cut(s) 1054, 1334, 1355, 2082, 2824
SmoI CTYRAG 5 cut(s) 1054, 1334, 1355, 2082, 2824
SnaBI TACGTA 1 cut(s) 2787
SpeI ACTAGT 1 cut(s) 1000
SspMI CTAG 4 cut(s) 374, 483, 705, 1001
StyD4I CCNGG 4 cut(s) 853, 1687, 1962, 2204
StyI CCWWGG 1 cut(s) 1995
TaiI ACGT 6 cut(s) 241, 1268, 1447, 1574, 2501, 2789
TaqII GACCGA 2 cut(s) 77, 2610
TatI WGTACW 3 cut(s) 111, 580, 1477
TauI GCSGC 2 cut(s) 953, 1397
TfiI GAWTC 8 cut(s) 270, 365, 728, 815, 1187, 1787, 2765, 2837
Tru1I TTAA 8 cut(s) 326, 876, 1169, 1356, 1367, 1731, 2508, 2528
Tru9I TTAA 8 cut(s) 326, 876, 1169, 1356, 1367, 1731, 2508, 2528
TseI GCWGC 5 cut(s) 50, 521, 1382, 2554, 2897
TspDTI ATGAA 8 cut(s) 638, 883, 1100, 1111, 1650, 1796, 2066, 2436
TspGWI ACGGA 3 cut(s) 330, 651, 2805
Vha464I CTTAAG 1 cut(s) 1355
VneI GTGCAC 1 cut(s) 660
VpaK11BI GGWCC 1 cut(s) 2829
VspI ATTAAT 1 cut(s) 1731
XapI RAATTY 3 cut(s) 439, 711, 1596
XbaI TCTAGA 1 cut(s) 373
XceI RCATGY 2 cut(s) 476, 1943
XcmI CCANNNNNNNNNTGG 2 cut(s) 1013, 2202
XhoI CTCGAG 1 cut(s) 2824
XmnI GAANNNNTTC 3 cut(s) 369, 1322, 2730
XspI CTAG 4 cut(s) 374, 483, 705, 1001
ZraI GACGTC 1 cut(s) 1266
ZrmI AGTACT 1 cut(s) 1479
Zsp2I ATGCAT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.