RLG00000010841

ER membrane protein complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0011093
Physical Location & Seq
Forward (+)
4723430 .. 4728472
5043 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010841

Sequence Viewer

Length: 2967 bp
ATGGCCACGACGAGCATGGCGGTTAGGGCTTTTCTTCTTCTACTGCTATTCCTATCTCTCGCAAATCTCACTCTTTCTATTTACGAAGATCAAGTCGGCCTCGTCGATTGGCACCAGCAGTACATAGGGAAAGTGAAGGACGCAGTGTTTCATACTCAAAAGAGTGGGCGAAAGCGTGTTGTGGTGTCCACTGAAGAGAATGTTATAGCATCACTTGATCTCCGACACGGGGAGATATTTTGGAGACATGTTCTTGGGAGCAATGATGTTGTCAATGGAATTGACATAGCATTGGGAAAATATGTCATTACCCTTTCGTCTGAAGGAAGTATTTTGAGAGCATGGAACCTTCCAGATGGTCAGATGGTGTGGCAATCTTTTATAGATGGCTCAGGGGCGTCAAAATCATTATTAACTGTCCCGACAAATTTGATCGTCAACAAGGAAAAGTTGATCCTTGTTTTTGGTAAAGGGTCTCTACATGCTGTTTCTGGCATTGATGGTGAGGTTCTTTGGACAAAGGATTTTGCAGCTGAAAGTCTAGAGGTTCAACATATTATTCAACCTGTTGGAAGTGAGGCAATCTATGTACTGGGATTTGTTGGTTCTTCCCAGTTTGATGCATATCAGATCAATCCTAAGAACGGAGAGATACTGAAGCACAATAGTGCAGCACTTTCTGGCGGCTATTCTGGGGAAGCAATATTGGCTTCGAGTGACATTCTTGTGACATTGGATGCCTCTAGGTCAAAGTTGGTAGTTATAAGCTTTCAGGATGGTGAACTTAACTTGCAACAGACATCCATATCTGATGTATTGGGGGATTCTTCTGGAACACCAGTACTGTTTTCTTTAAAACTACCAGGAATGTTTTCCGTAAAGCTTGATGGAGGAGTGACACTCATAAAAGTGACTGTAGAAGCCAAGTTGGAGGTGATGGATAAAATAAGTAGTGTGGCAGCTATTAGTGATGCAATTCCCCTTACTGAAGGTCAACAAGCTTTCGCACTAGTTCAACATGGAGATGGTAAGATTCATCTTACAGTGAAGCTTAGTCACGATCTGAGTGGTGATTTGCTGAAGGAGAGTATAGTTATGGACAATCAGAGGGGGATGGTGCATAAGGTTTTTATAAACAGTTATATACGGACAGATAGATCCAATGGGTTTAGGGCCTTGATTGTCATGGAGGATCATTCACTTTTGTTGTTACAACAAGGTGCCATTGTCTGGAGTAGGGAGGATGGTCTTGCCTCAATTGTTGATGTACTAACATCAGAACTACCCGTGGAAAAGGAAGGTGTATCTGTAGCAAAAGTTGAGGAAAACCTTTTTGAGTGGCTTAAGGGACACCTACTGAAGCTTAAGGGAACCTTAATGCTTGCAAGTGCTGATGATGTTGCTGCTATACAAGAGAGGAGGTTAAAAAGTTCTGAAAAGAGCAAACTGACCCGAGACCATAATGGTTTTCGGAAGCTTCTCATAGTACTTACGAGAGCGGGAAAGCTCTTTGCCTTGCACACTGGATATGGACAAGTTGTCTGGTCTCTGTTACTGCCTAATCTGCGTAAATCTGAGTGCGAGTTTCCGACTGGTTTGAATATTTATCAGTGGCAGGTACCCCATCATCACGCAATGGATGAAGATCCATCTATCCTCATAGTAGGTCAATGTGGACAAAGTTCTGATGCACCAGGTGTTCTGTCTATTGTTGATGCATATACTGGCACTGAAATTAATTCAATGGATCTCGTACATTCCATTTCGCAAGTTATTCCACTGCCATTTACTGATTCAACAGAACAGCGACTTCATCTACTAATAGATGGAAACCAGCATGCATATCTATATCCAAGAACATCCGAGGCCATTGATATCTTTCAACGCGAGTTCTTAAACATATACTGGTACTCAGTTGAGACTAAGAATGGCATTATCAAAGGACATGTCTTGAAGAGCTATTGCATCCAGGAAGTTGTAGACAGCTATTGCTTCGAGTCCAGGGATATATGGTCAATTGTATTCCCATCTGACTCAGAAAAGATCATTGCAACAGTGACAAGAAAACCAAATGAGGTGGTTCACACTCAAGCAAAGGTTATAGCTGCTGAAGAAGATCTGATGTATAAGTATATATCTAAGAATCTACTCTTTGTTGCAACTGTTGCACCTAAAGGCACTGGTGCAATTGGAACAGCTACCCCAGAGGAGTCATGGTTGACTGTATATCTTATCGATACTGTAACTGGTCGTATATTGCATCGGATGACCCATCATGGTGCACAGGGACCTGTCCACGCTGTTTTCAGTGAGAATTGGGTTGTATACCACTACTTTAATCTTAGAGCACACAGATATGAGATGTCAGTCATAGAGATATATGATCAGTCCCGAGCGGACAATAAGGATGTTTGGAAGCTGATTCTTGGGAAACATAATCTCACTTCACCTATTTCTTCATATTCCCGACCAGAGGTTGTGACGAAATCACAGTCTTACTTTTTCACGTATTCTGTAAAAGCAATAGATGTGACATTGACAGCGAAGGGTATTACTTCAAAGCAGCTTCTTATTGGTACAATTGGGGATCAGGTTTTGGCACTTGACAAGCGATTTTTGGATCCCCGTCGATCAGTCAACCCCACACCAGCTGAGAAAGAGGAAGGCATTATACCTCTAACAGATTCATTGCCAATAATTCCTCAGTCCTATGTGACGCATGCTCTAAGAGTCGAAGGACTCCGAGGCATAGTGACAGTACCTGCCAAGCTGGAGTCAACAACCCTTGTCTTCGTATATGGAGTGGATCTGTATTTTACTCAACTCGCACCGTCAAGGACCTACGATTCGCTGACCGATGATTTCAGCTATGCATTGCTTCTCATCACCATCGTTGCACTCATAGCAGCAATCTTTGTAACCTGGATTTTATCCGAGAAAAAAGAACTACGAGAGAAGTGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1620 PQQ_2

Protein Analysis

989

Amino Acids

109.53

Weight (kDa)

5.96

Isoelectric Point (pI)

31.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EMC1_N PF25293 27 - 415 1.4e-136 EMC1 N-terminal beta-propeller domain
PQQ_2 PF13360 54 - 261 7.8e-10 Outer membrane protein assembly factor BamB
Beta-prop_ACSF4 PF13570 56 - 178 2.8e-06 Beta-alanine-activating enzyme, beta-propeller
EMC1_C PF07774 770 - 987 1.1e-67 ER membrane protein complex subunit 1, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11560
fragaria_vesca FvH4_2g35850
malus_domestica MD08G1115900.v1.1 MD15G1095600.v1.1
prunus_persica Prupe.1G450600_v2.0.a1 Prupe.1G450600_v2.0.a1
pyrus_communis pycom08g09670 pycom08g09680 pycom15g08790
rosa_chinensis RchiOBHm_Chr6g0305971
rosa_laevigata RLG00000010841
rosa_multiflora Rmu_co8311943.1_g000001 Rmu_sc0001809.1_g000056
rosa_roxburghii Rroxscaffold_7G00162180
rosa_rugosa Rorug06G0345700
rosa_samantha Rh6AG457600 Rh6BG443500 Rh6CG471700 Rh6DG458100
rosa_wichuraiana Rw6G039920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 764, 1133
Acc36I ACCTGC 2 cut(s) 1606, 2770
Acc65I GGTACC 1 cut(s) 1618
AccB1I GGYRCC 3 cut(s) 111, 1220, 1618
AccB7I CCANNNNNTGG 1 cut(s) 1230
AccBSI CCGCTC 2 cut(s) 1499, 2396
AccI GTMKAC 2 cut(s) 1980, 2325
AccII CGCG 1 cut(s) 1887
AciI CCGC 4 cut(s) 20, 684, 1499, 2396
AclWI GGATC 9 cut(s) 448, 1152, 1200, 1640, 1755, 2595, 2615, 2628, 2814
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 427
AcuI CTGAAG 7 cut(s) 213, 342, 677, 1008, 1100, 1379, 2130
AcyI GRCGYC 1 cut(s) 398
AdeI CACNNNGTG 1 cut(s) 1697
AfiI CCNNNNNNNGG 4 cut(s) 229, 644, 1230, 2473
AflII CTTAAG 2 cut(s) 1343, 1364
AflIII ACRYGT 2 cut(s) 247, 1945
AgsI TTSAA 9 cut(s) 551, 563, 1016, 1600, 1743, 1797, 1883, 1954, 2559
AhdI GACNNNNNGTC 1 cut(s) 1538
AhlI ACTAGT 1 cut(s) 1009
AjnI CCWGG 5 cut(s) 862, 1693, 1968, 2000, 2921
AleI CACNNNNGTG 1 cut(s) 666
Alw21I GWGCWC 2 cut(s) 2284, 2350
Alw26I GTCTC 5 cut(s) 238, 480, 1449, 1551, 1913
Alw44I GTGCAC 1 cut(s) 2280
AlwI GGATC 9 cut(s) 448, 1152, 1200, 1640, 1755, 2595, 2615, 2628, 2814
AlwNI CAGNNNCTG 1 cut(s) 2762
Ama87I CYCGRG 2 cut(s) 1452, 2391
AoxI GGCC 4 cut(s) 3, 97, 1173, 1866
ApaLI GTGCAC 1 cut(s) 2280
ApeKI GCWGC 7 cut(s) 530, 671, 959, 1403, 2105, 2563, 2906
ApoI RAATTY 1 cut(s) 427
ArsI GACNNNNNNTTYG 8 cut(s) 78, 110, 1932, 1964, 2477, 2509, 2830, 2862
AseI ATTAAT 1 cut(s) 1737
Asp700I GAANNNNTTC 1 cut(s) 871
Asp718I GGTACC 1 cut(s) 1618
AspS9I GGNCC 3 cut(s) 1173, 2288, 2838
AsuHPI GGTGA 6 cut(s) 515, 791, 946, 1082, 2439, 2878
AvaI CYCGRG 2 cut(s) 1452, 2391
AvaII GGWCC 2 cut(s) 2288, 2838
BaeGI GKGCMC 1 cut(s) 2284
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 2620
BanI GGYRCC 3 cut(s) 111, 1220, 1618
BarI GAAGNNNNNNTAC 2 cut(s) 2655, 2687
BbsI GAAGAC 1 cut(s) 2782
Bbv12I GWGCWC 2 cut(s) 2284, 2350
BbvI GCAGC 7 cut(s) 542, 683, 971, 1390, 2092, 2575, 2918
BciT130I CCWGG 5 cut(s) 864, 1695, 1970, 2002, 2923
BclI TGATCA 1 cut(s) 2383
BcoDI GTCTC 5 cut(s) 238, 480, 1449, 1551, 1913
BcuI ACTAGT 1 cut(s) 1009
BfaI CTAG 3 cut(s) 542, 744, 1010
BfmI CTRYAG 2 cut(s) 915, 1308
BfrI CTTAAG 2 cut(s) 1343, 1364
BfuAI ACCTGC 2 cut(s) 1606, 2770
BglII AGATCT 1 cut(s) 2116
BisI GCNGC 8 cut(s) 531, 672, 685, 960, 1404, 2106, 2564, 2907
BlsI GCNGC 8 cut(s) 532, 673, 686, 961, 1405, 2107, 2565, 2908
BmcAI AGTACT 2 cut(s) 843, 1488
Bme1390I CCNGG 5 cut(s) 864, 1695, 1970, 2002, 2923
Bme18I GGWCC 2 cut(s) 2288, 2838
BmeRI GACNNNNNGTC 1 cut(s) 1538
BmeT110I CYCGRG 2 cut(s) 1452, 2391
BmgT120I GGNCC 3 cut(s) 1173, 2288, 2838
BmiI GGNNCC 7 cut(s) 113, 347, 1222, 1372, 1620, 2289, 2622
BmrFI CCNGG 5 cut(s) 864, 1695, 1970, 2002, 2923
BmrI ACTGGG 2 cut(s) 602, 607
BmsI GCATC 8 cut(s) 218, 610, 727, 961, 1678, 1705, 1974, 2269
BmuI ACTGGG 2 cut(s) 602, 607
BpiI GAAGAC 1 cut(s) 2782
BplI GAGNNNNNCTC 2 cut(s) 885, 917
BpmI CTGGAG 2 cut(s) 1252, 2792
Bpu10I CCTNAGC 1 cut(s) 391
BpuEI CTTGAG 1 cut(s) 2073
Bsa29I ATCGAT 1 cut(s) 2235
BsaAI YACGTR 1 cut(s) 2508
BsaBI GATNNNNATC 2 cut(s) 624, 1644
BsaHI GRCGYC 1 cut(s) 398
BsaI GGTCTC 3 cut(s) 480, 1449, 1551
BsaJI CCNNGG 4 cut(s) 1287, 1863, 2001, 2743
BsaXI ACNNNNNCTCC 4 cut(s) 204, 234, 2201, 2231
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 644, 1230, 2473
Bse1I ACTGG 9 cut(s) 597, 613, 839, 1528, 1597, 1729, 1910, 2185, 2251
Bse3DI GCAATG 5 cut(s) 268, 1641, 2046, 2687, 2873
Bse8I GATNNNNATC 2 cut(s) 624, 1644
BseBI CCWGG 5 cut(s) 864, 1695, 1970, 2002, 2923
BseCI ATCGAT 1 cut(s) 2235
BseDI CCNNGG 4 cut(s) 1287, 1863, 2001, 2743
BseJI GATNNNNATC 2 cut(s) 624, 1644
BseLI CCNNNNNNNGG 4 cut(s) 229, 644, 1230, 2473
BseMI GCAATG 5 cut(s) 268, 1641, 2046, 2687, 2873
BseMII CTCAG 7 cut(s) 405, 1055, 1566, 1926, 2049, 2643, 2717
BseNI ACTGG 9 cut(s) 597, 613, 839, 1528, 1597, 1729, 1910, 2185, 2251
BseRI GAGGAG 3 cut(s) 906, 1432, 2222
BseSI GKGCMC 1 cut(s) 2284
BseXI GCAGC 7 cut(s) 542, 683, 971, 1390, 2092, 2575, 2918
BsgI GTGCAG 1 cut(s) 690
Bsh1236I CGCG 1 cut(s) 1887
BshFI GGCC 4 cut(s) 5, 99, 1175, 1868
BshNI GGYRCC 3 cut(s) 111, 1220, 1618
BshVI ATCGAT 1 cut(s) 2235
BsiHKAI GWGCWC 2 cut(s) 2284, 2350
BsiHKCI CYCGRG 2 cut(s) 1452, 2391
BslFI GGGAC 4 cut(s) 404, 1362, 2301, 2374
BslI CCNNNNNNNGG 4 cut(s) 229, 644, 1230, 2473
BsmAI GTCTC 5 cut(s) 238, 480, 1449, 1551, 1913
BsmFI GGGAC 4 cut(s) 404, 1362, 2301, 2374
BsnI GGCC 4 cut(s) 5, 99, 1175, 1868
Bso31I GGTCTC 3 cut(s) 480, 1449, 1551
BsoBI CYCGRG 2 cut(s) 1452, 2391
Bsp1286I GDGCHC 2 cut(s) 2284, 2350
BspACI CCGC 4 cut(s) 20, 684, 1499, 2396
BspANI GGCC 4 cut(s) 5, 99, 1175, 1868
BspCNI CTCAG 7 cut(s) 404, 1056, 1567, 1925, 2048, 2644, 2716
BspDI ATCGAT 1 cut(s) 2235
BspFNI CGCG 1 cut(s) 1887
BspLI GGNNCC 7 cut(s) 113, 347, 1222, 1372, 1620, 2289, 2622
BspMI ACCTGC 2 cut(s) 1606, 2770
BspPI GGATC 9 cut(s) 448, 1152, 1200, 1640, 1755, 2595, 2615, 2628, 2814
BspQI GCTCTTC 1 cut(s) 1949
BspT107I GGYRCC 3 cut(s) 111, 1220, 1618
BspTI CTTAAG 2 cut(s) 1343, 1364
BspTNI GGTCTC 3 cut(s) 480, 1449, 1551
BsrBI CCGCTC 2 cut(s) 1499, 2396
BsrDI GCAATG 5 cut(s) 268, 1641, 2046, 2687, 2873
BsrI ACTGG 9 cut(s) 597, 613, 839, 1528, 1597, 1729, 1910, 2185, 2251
BssECI CCNNGG 4 cut(s) 1287, 1863, 2001, 2743
BssNAI GTATAC 1 cut(s) 2326
BssNI GRCGYC 1 cut(s) 398
Bst1107I GTATAC 1 cut(s) 2326
Bst2UI CCWGG 5 cut(s) 864, 1695, 1970, 2002, 2923
Bst6I CTCTTC 2 cut(s) 189, 1949
BstACI GRCGYC 1 cut(s) 398
BstAFI CTTAAG 2 cut(s) 1343, 1364
BstAPI GCANNNNNTGC 1 cut(s) 2165
BstBAI YACGTR 1 cut(s) 2508
BstC8I GCNNGC 3 cut(s) 1383, 1839, 2721
BstDSI CCRYGG 1 cut(s) 1287
BstFNI CGCG 1 cut(s) 1887
BstMAI GTCTC 5 cut(s) 238, 480, 1449, 1551, 1913
BstMWI GCNNNNNNNGC 5 cut(s) 26, 707, 1564, 2165, 2903
BstNI CCWGG 5 cut(s) 864, 1695, 1970, 2002, 2923
BstNSI RCATGY 5 cut(s) 251, 485, 1841, 1949, 2723
BstSCI CCNGG 5 cut(s) 862, 1693, 1968, 2000, 2921
BstSFI CTRYAG 2 cut(s) 915, 1308
BstSLI GKGCMC 1 cut(s) 2284
BstUI CGCG 1 cut(s) 1887
BstV1I GCAGC 7 cut(s) 542, 683, 971, 1390, 2092, 2575, 2918
BstV2I GAAGAC 1 cut(s) 2782
BstX2I RGATCY 6 cut(s) 1157, 1645, 1747, 2116, 2620, 2806
BstYI RGATCY 6 cut(s) 1157, 1645, 1747, 2116, 2620, 2806
BstZ17I GTATAC 1 cut(s) 2326
Bsu15I ATCGAT 1 cut(s) 2235
BsuRI GGCC 4 cut(s) 5, 99, 1175, 1868
BsuTUI ATCGAT 1 cut(s) 2235
BtgI CCRYGG 1 cut(s) 1287
BtsI GCAGTG 2 cut(s) 150, 1778
BveI ACCTGC 2 cut(s) 1606, 2770
Cac8I GCNNGC 3 cut(s) 1383, 1839, 2721
CaiI CAGNNNCTG 1 cut(s) 2762
Cfr13I GGNCC 3 cut(s) 1173, 2288, 2838
ClaI ATCGAT 1 cut(s) 2235
CseI GACGC 3 cut(s) 149, 387, 2725
CsiI ACCWGGT 1 cut(s) 1693
CspCI CAANNNNNGTGG 2 cut(s) 2058, 2093
DraI TTTAAA 1 cut(s) 855
DraIII CACNNNGTG 1 cut(s) 1697
DriI GACNNNNNGTC 1 cut(s) 1538
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 189, 1949
Eam1105I GACNNNNNGTC 1 cut(s) 1538
EarI CTCTTC 2 cut(s) 189, 1949
Eco31I GGTCTC 3 cut(s) 480, 1449, 1551
Eco32I GATATC 1 cut(s) 1876
Eco47I GGWCC 2 cut(s) 2288, 2838
Eco57I CTGAAG 7 cut(s) 213, 342, 677, 1008, 1100, 1379, 2130
Eco88I CYCGRG 2 cut(s) 1452, 2391
EcoO109I RGGNCCY 3 cut(s) 1173, 2288, 2838
EcoRII CCWGG 5 cut(s) 862, 1693, 1968, 2000, 2921
EcoRV GATATC 1 cut(s) 1876
EcoT22I ATGCAT 4 cut(s) 625, 1720, 1843, 2875
FalI AAGNNNNNCTT 2 cut(s) 1358, 1390
FaqI GGGAC 4 cut(s) 404, 1362, 2301, 2374
FauI CCCGC 1 cut(s) 1492
FbaI TGATCA 1 cut(s) 2383
FblI GTMKAC 2 cut(s) 1980, 2325
Fnu4HI GCNGC 8 cut(s) 531, 672, 685, 960, 1404, 2106, 2564, 2907
Fsp4HI GCNGC 8 cut(s) 531, 672, 685, 960, 1404, 2106, 2564, 2907
FspBI CTAG 3 cut(s) 542, 744, 1010
GluI GCNGC 8 cut(s) 531, 672, 685, 960, 1404, 2106, 2564, 2907
GsuI CTGGAG 2 cut(s) 1252, 2792
HaeIII GGCC 4 cut(s) 5, 99, 1175, 1868
HgaI GACGC 3 cut(s) 149, 387, 2725
Hin1I GRCGYC 1 cut(s) 398
HincII GTYRAC 5 cut(s) 439, 995, 2220, 2638, 2778
HindII GTYRAC 5 cut(s) 439, 995, 2220, 2638, 2778
HindIII AAGCTT 6 cut(s) 766, 881, 999, 1049, 1361, 1475
HphI GGTGA 6 cut(s) 515, 791, 946, 1082, 2439, 2878
Hpy99I CGWCG 3 cut(s) 13, 107, 2631
HpyAV CCTTC 9 cut(s) 130, 317, 359, 983, 1075, 1292, 2539, 2657, 2729
HpyCH4IV ACGT 1 cut(s) 2507
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 707, 1564, 2165, 2903
HpySE526I ACGT 1 cut(s) 2507
Hsp92I GRCGYC 1 cut(s) 398
KpnI GGTACC 1 cut(s) 1622
Ksp22I TGATCA 1 cut(s) 2383
LguI GCTCTTC 1 cut(s) 1949
LmnI GCTCC 1 cut(s) 258
Lsp1109I GCAGC 7 cut(s) 542, 683, 971, 1390, 2092, 2575, 2918
LweI GCATC 8 cut(s) 218, 610, 727, 961, 1678, 1705, 1974, 2269
MabI ACCWGGT 1 cut(s) 1693
MaeI CTAG 3 cut(s) 542, 744, 1010
MaeII ACGT 1 cut(s) 2507
MbiI CCGCTC 2 cut(s) 1499, 2396
MfeI CAATTG 4 cut(s) 1257, 2016, 2187, 2580
MflI RGATCY 6 cut(s) 1157, 1645, 1747, 2116, 2620, 2806
MhlI GDGCHC 2 cut(s) 2284, 2350
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 6 cut(s) 2006, 2027, 2219, 2733, 2739, 2783
MmeI TCCRAC 4 cut(s) 247, 550, 909, 1613
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 4 cut(s) 625, 1720, 1843, 2875
MroXI GAANNNNTTC 1 cut(s) 871
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 6 cut(s) 666, 725, 908, 1023, 2277, 2355
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 533, 2651
MspCI CTTAAG 2 cut(s) 1343, 1364
MspR9I CCNGG 5 cut(s) 864, 1695, 1970, 2002, 2923
MunI CAATTG 4 cut(s) 1257, 2016, 2187, 2580
MvaI CCWGG 5 cut(s) 864, 1695, 1970, 2002, 2923
MvnI CGCG 1 cut(s) 1887
MwoI GCNNNNNNNGC 5 cut(s) 26, 707, 1564, 2165, 2903
NlaIV GGNNCC 7 cut(s) 113, 347, 1222, 1372, 1620, 2289, 2622
NsiI ATGCAT 4 cut(s) 625, 1720, 1843, 2875
NspI RCATGY 5 cut(s) 251, 485, 1841, 1949, 2723
OliI CACNNNNGTG 1 cut(s) 666
PaeI GCATGC 2 cut(s) 1841, 2723
PciI ACATGT 2 cut(s) 247, 1945
PciSI GCTCTTC 1 cut(s) 1949
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 871
PfeI GAWTC 7 cut(s) 824, 1033, 1793, 2143, 2422, 2684, 2846
PflMI CCANNNNNTGG 1 cut(s) 1230
PfoI TCCNGGA 1 cut(s) 1968
PkrI GCNGC 8 cut(s) 532, 673, 686, 961, 1405, 2107, 2565, 2908
PleI GAGTC 6 cut(s) 2005, 2027, 2218, 2733, 2738, 2782
PpsI GAGTC 6 cut(s) 2005, 2027, 2218, 2733, 2738, 2782
Ppu21I YACGTR 1 cut(s) 2508
PpuMI RGGWCCY 2 cut(s) 2288, 2838
PscI ACATGT 2 cut(s) 247, 1945
PshBI ATTAAT 1 cut(s) 1737
PsiI TTATAA 2 cut(s) 764, 1133
Psp5II RGGWCCY 2 cut(s) 2288, 2838
Psp6I CCWGG 5 cut(s) 862, 1693, 1968, 2000, 2921
PspGI CCWGG 5 cut(s) 862, 1693, 1968, 2000, 2921
PspN4I GGNNCC 7 cut(s) 113, 347, 1222, 1372, 1620, 2289, 2622
PspPI GGNCC 3 cut(s) 1173, 2288, 2838
PspPPI RGGWCCY 2 cut(s) 2288, 2838
PstNI CAGNNNCTG 1 cut(s) 2762
PsuI RGATCY 6 cut(s) 1157, 1645, 1747, 2116, 2620, 2806
PvuII CAGCTG 2 cut(s) 533, 2651
RseI CAYNNNNRTG 6 cut(s) 666, 725, 908, 1023, 2277, 2355
SapI GCTCTTC 1 cut(s) 1949
SatI GCNGC 8 cut(s) 531, 672, 685, 960, 1404, 2106, 2564, 2907
Sau96I GGNCC 3 cut(s) 1173, 2288, 2838
ScaI AGTACT 2 cut(s) 843, 1488
SchI GAGTC 6 cut(s) 2006, 2027, 2219, 2733, 2739, 2783
ScrFI CCNGG 5 cut(s) 864, 1695, 1970, 2002, 2923
SduI GDGCHC 2 cut(s) 2284, 2350
SexAI ACCWGGT 1 cut(s) 1693
SfaNI GCATC 8 cut(s) 218, 610, 727, 961, 1678, 1705, 1974, 2269
SfcI CTRYAG 2 cut(s) 915, 1308
SinI GGWCC 2 cut(s) 2288, 2838
SmiMI CAYNNNNRTG 6 cut(s) 666, 725, 908, 1023, 2277, 2355
SmlI CTYRAG 3 cut(s) 1343, 1364, 2088
SmoI CTYRAG 3 cut(s) 1343, 1364, 2088
SpeI ACTAGT 1 cut(s) 1009
SphI GCATGC 2 cut(s) 1841, 2723
SsiI CCGC 4 cut(s) 20, 684, 1499, 2396
SspI AATATT 2 cut(s) 705, 1603
SspMI CTAG 3 cut(s) 542, 744, 1010
StyD4I CCNGG 5 cut(s) 862, 1693, 1968, 2000, 2921
TaiI ACGT 1 cut(s) 2510
TaqI TCGA 6 cut(s) 105, 713, 1995, 2235, 2629, 2733
TaqII GACCGA 1 cut(s) 2870
TatI WGTACW 5 cut(s) 120, 589, 841, 1267, 1486
TauI GCSGC 1 cut(s) 687
TfiI GAWTC 7 cut(s) 824, 1033, 1793, 2143, 2422, 2684, 2846
TseI GCWGC 7 cut(s) 530, 671, 959, 1403, 2105, 2563, 2906
TspDTI ATGAA 6 cut(s) 140, 1025, 1656, 1802, 2448, 2676
TspGWI ACGGA 3 cut(s) 660, 865, 1162
Van91I CCANNNNNTGG 1 cut(s) 1230
Vha464I CTTAAG 2 cut(s) 1343, 1364
VneI GTGCAC 1 cut(s) 2280
VpaK11BI GGWCC 2 cut(s) 2288, 2838
VspI ATTAAT 1 cut(s) 1737
XapI RAATTY 1 cut(s) 427
XbaI TCTAGA 1 cut(s) 541
XceI RCATGY 5 cut(s) 251, 485, 1841, 1949, 2723
XcmI CCANNNNNNNNNTGG 2 cut(s) 13, 2211
XmiI GTMKAC 2 cut(s) 1980, 2325
XmnI GAANNNNTTC 1 cut(s) 871
XspI CTAG 3 cut(s) 542, 744, 1010
ZrmI AGTACT 2 cut(s) 843, 1488
Zsp2I ATGCAT 4 cut(s) 625, 1720, 1843, 2875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.