Rh6BG443500

ER membrane protein complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0011093
Physical Location & Seq
Forward (+)
66049174 .. 66054223
5050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG443500.1

Sequence Viewer

Length: 2973 bp
ATGGCCACGACGAGCATGGCGGTTAGGGATTTTCTTCTTCTTCTACTACTGGTATTCCTATCTCTCGCAAATCTCACTCTTTCGCTTTACGAAGATCAAGTCGGCCTCGTCGATTGGCACCAACAATATATAGGGAAAGTGAAGGACGCAGTGTTTCATACTCAAAAGAGTGGGCGAAAGCGTGTTGTGGTGTCCACTGAAGAGAATGTTATAGCATCACTTGATCTCCGACACGGGGAGATATTTTGGAGACATGTTCTCGGGAGCAATGATGTTGTCGATGGAATTGACATAGCATTGGGAAAATATGTCATTACCCTTTCGTCTGAAGGAAGTACTTTGAGAGCATGGAACCTTCCAGATGGTCAGATGGTGTGGCAATCTTTTATAGATGGCTCAGGGGCCTCAAAATCATTATTAACTGTCCCGACAAATTTGATCGTCAACAAGGAAAATTTGATCCTTGTTTTTGGTAAAGGGTCTCTACATGCTGTTTCTGGCATTGATGGTGAGGTTCTTTGGACAAAGGATTTTGCAGCTGAAAGTCTAGAGGTTCAACATATTATTCAACCTGTTGGAAGTGAGGCAATCTATGTACTAGGATTTGTTGGTTCTTCCCAGTTTGATGCATATCAGATCAATCCTAAGAACGGAGAGATACTGAAGCACAATAGTGCAGCACTTTCTGGCGGCTATTCTGGGGAAGCAATATTGGCTTCGAGTGACATTCTTGTGACATTGGATGCCTCTAGGTCAAAGTTGGTAGTTATAAGCTTTCAGGATGGTGAACTTAACTTGCAACAGACATCCATATCTGATATATTGGGGGATTCTTCTGGAACACCAGTACTGTTATCTTCAAAACTACCAGGAATGTTTTCCGTAAAGCTTGATGGAGGAGTGACACTCATAAAAGTGACTGTAGAAGCCAAGTTGGAGGTGATGGATAAAATAAGTAGTGTGGTAGCTATTAGTGATGCAATTCCCCTTACTGAAGGTCAACAAGCTTTCGCACTAGTTCAACATGGAGATGGTAAGATTCATCTTACAGTGAAGCTTAGTCACGATCTGAGTGGTGATTTGCTGAAGGAGAGTATAGTTATGGACAATCAGAGGGGGATGGTGCATAAGGTTTTTATAAACAGTTATATCCGGACAGACAGATCCAATGGGTTTAGGGCCTTGATTGTCATGGAGGATCATTCACTTTTGTTGTTACAACAAGGTGCCATTGTCTGGAGTAGGGAGGATGGTCTTGCCTCAATTGTTGATGTACTAACATCAGAACTACCCGTGGAAAAGGAAGGTGTATCTGTAGCAAAAGTTGAGGAAAACCTTTTTGAGTGGCTTAAGGGACACCTACTGAAGCTTAAGGGAACCTTAATGCTTGCAAGTGCTGATGATGTTGCTGCTATACAAGAGAAGAGGTTAAAAAGTTCTGAAAAGAGCAAACTGACCCGAGACCATAATGGTTTTCGGAAGCTTCTCATAGTACTTACGAGAGCGGGAAAGCTCTTTGCCTTGCACACTGGATATGGACAAGTTGTCTGGTCTCTGTTACTGCCTAATCTGCGTAAATCTGAGTGCGAGTTTCCGACTGGTTTGAATATTTATCAGTGGCAGGTACCCCATCATCACGCAATGGATGAAGATCCATCTATCCTCATAGTAGGTCGATGTGGGCAAAGTTCTGATGCACCAGGTGTTCTGTCTATTGTTGATGCATATACTGGCACTGAAATTAATTCAATGGATCTCGTACATTCCATTTCTCAAGTTATTCCACTGCCATTTACTGATTCAACAGAACAGCGACTTCATCTACTAATAGATGGAAACCAGCATGCATATCTATATCCAAGAACATCCGAGGCCATTGATATCTTTCAACGCGAGTTCTCAAACATATACTGGTACTCTGTTGAGACTAAGAATGGCATTATCAAAGGACATGTCTTGAAGAGCAATTGCATCCAGGAAGTTGTAGACAACTATTGCTTCGAGTCCAGGGATATATGGTCAATTGTATTCCCATCTGACTCAGAAAAGATCATTGCAACAGTGACAAGAAAACCAAATGAGGTGGTTCACACTCAAGCAAAGGTTATAGCTGCTGAAGAAGATCTGATGTATAAGTATATATCTAAGAATCTACTCTTTGTTGCAACTGTTGCACCTAAAGGCAGTGGTGCAATTGGAACAGCTACCCCAGAGGAGTCGTGGTTGACTGTATATCTTATCGATACTGTAACTGGTCGTATATTGCATCGGATGACCCATCATGGTGCACAGGGACCTGTCCACGCTGTTTTCAGTGAGAATTGGGTTGTATACCACTACTTTAATCTTAGAGCACACAGATATGAGATGTCAGTCATAGAGATATATGATCAGTCCCGTGCGGACAATAAGGATGTTTGGAAGCTGATTCTTGGGAAACATAATCTCACTTCACCTATTTCTTCATATTCCCGACCAGAGGTTGTGACGAAATCACAGTCTTCCTTTTTCACGTATTCTGTAAAAGCAATAGATGTGACATTGACAGCAAAGGGTATTACTTCAAAGCAGCTTCTTATTGGTACAATTGGGGATCAGGTTTTGGCACTTGACAAGCGATTTTTGGATCCCCGTCGATCAGTCAACCCCACACCAGCTGAGAAAGAGGAAGGCATTATACCTCTAACAGATTCATTGCCAATAATTCCTCAGTCCTATGTGACACATGCTCTAAGAGTCGAAGGACTCCGAGGCATAGTGGCAGTACCTGCCAAGCTGGAGTCAACAACCCTTGTCTTCGTATATGGAGTGGATCTGTTTTTTACTCAACTCGCACCGTCAAGGACCTACGATTCGCTGACTGATGATTTCAGCTATGCATTGCTTCTCATCACCATCGTTGCACTCATAGCAGCAATCTTTGTAACCTGGATTTTATCCGAGAAAAAAGAACTACGAGAGAAGTGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1620 PQQ_2

Protein Analysis

990

Amino Acids

109.56

Weight (kDa)

5.88

Isoelectric Point (pI)

31.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EMC1_N PF25293 29 - 417 6.8e-136 EMC1 N-terminal beta-propeller domain
PQQ_2 PF13360 57 - 262 7.4e-10 Outer membrane protein assembly factor BamB
Beta-prop_ACSF4 PF13570 58 - 180 3.8e-06 Beta-alanine-activating enzyme, beta-propeller
PQQ_2 PF13360 113 - 287 3.4e-06 Outer membrane protein assembly factor BamB
EMC1_C PF07774 772 - 989 5.8e-66 ER membrane protein complex subunit 1, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11560
fragaria_vesca FvH4_2g35850
malus_domestica MD08G1115900.v1.1 MD15G1095600.v1.1
prunus_persica Prupe.1G450600_v2.0.a1 Prupe.1G450600_v2.0.a1
pyrus_communis pycom08g09670 pycom08g09680 pycom15g08790
rosa_chinensis RchiOBHm_Chr6g0305971
rosa_laevigata RLG00000010841
rosa_multiflora Rmu_co8311943.1_g000001 Rmu_sc0001809.1_g000056
rosa_roxburghii Rroxscaffold_7G00162180
rosa_rugosa Rorug06G0345700
rosa_samantha Rh6AG457600 Rh6BG443500 Rh6CG471700 Rh6DG458100
rosa_wichuraiana Rw6G039920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 770, 1139
Acc36I ACCTGC 2 cut(s) 1612, 2776
Acc65I GGTACC 1 cut(s) 1624
AccB1I GGYRCC 3 cut(s) 117, 1226, 1624
AccB7I CCANNNNNTGG 1 cut(s) 1236
AccBSI CCGCTC 1 cut(s) 1505
AccI GTMKAC 2 cut(s) 1986, 2331
AccII CGCG 1 cut(s) 1893
AccIII TCCGGA 1 cut(s) 1152
AciI CCGC 4 cut(s) 20, 690, 1505, 2402
AclWI GGATC 9 cut(s) 454, 1158, 1206, 1646, 1761, 2601, 2621, 2634, 2820
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 433, 454
AcuI CTGAAG 7 cut(s) 219, 348, 683, 1014, 1106, 1385, 2136
AdeI CACNNNGTG 1 cut(s) 1703
AfiI CCNNNNNNNGG 4 cut(s) 235, 650, 1236, 2479
AflII CTTAAG 2 cut(s) 1349, 1370
AflIII ACRYGT 2 cut(s) 253, 1951
AhdI GACNNNNNGTC 1 cut(s) 1544
AhlI ACTAGT 1 cut(s) 1015
AjnI CCWGG 5 cut(s) 868, 1699, 1974, 2006, 2927
AleI CACNNNNGTG 1 cut(s) 672
Alw21I GWGCWC 2 cut(s) 2290, 2356
Alw26I GTCTC 5 cut(s) 244, 486, 1455, 1557, 1919
Alw44I GTGCAC 1 cut(s) 2286
AlwI GGATC 9 cut(s) 454, 1158, 1206, 1646, 1761, 2601, 2621, 2634, 2820
AlwNI CAGNNNCTG 1 cut(s) 2768
Ama87I CYCGRG 2 cut(s) 260, 1458
Aor13HI TCCGGA 1 cut(s) 1152
AoxI GGCC 5 cut(s) 3, 103, 402, 1179, 1872
ApaLI GTGCAC 1 cut(s) 2286
ApeKI GCWGC 6 cut(s) 536, 677, 1409, 2111, 2569, 2912
ApoI RAATTY 2 cut(s) 433, 454
ArsI GACNNNNNNTTYG 8 cut(s) 84, 116, 1938, 1970, 2483, 2515, 2836, 2868
AseI ATTAAT 1 cut(s) 1743
Asp700I GAANNNNTTC 1 cut(s) 877
Asp718I GGTACC 1 cut(s) 1624
AspS9I GGNCC 4 cut(s) 402, 1179, 2294, 2844
AsuHPI GGTGA 6 cut(s) 521, 797, 952, 1088, 2445, 2884
AvaI CYCGRG 2 cut(s) 260, 1458
AvaII GGWCC 2 cut(s) 2294, 2844
BaeGI GKGCMC 1 cut(s) 2290
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 2626
BanI GGYRCC 3 cut(s) 117, 1226, 1624
BarI GAAGNNNNNNTAC 2 cut(s) 2661, 2693
BbsI GAAGAC 2 cut(s) 2493, 2788
Bbv12I GWGCWC 2 cut(s) 2290, 2356
BbvI GCAGC 6 cut(s) 548, 689, 1396, 2098, 2581, 2924
BciT130I CCWGG 5 cut(s) 870, 1701, 1976, 2008, 2929
BclI TGATCA 1 cut(s) 2389
BcoDI GTCTC 5 cut(s) 244, 486, 1455, 1557, 1919
BcuI ACTAGT 1 cut(s) 1015
BfaI CTAG 4 cut(s) 548, 599, 750, 1016
BfmI CTRYAG 2 cut(s) 921, 1314
BfrI CTTAAG 2 cut(s) 1349, 1370
BfuAI ACCTGC 2 cut(s) 1612, 2776
BglII AGATCT 1 cut(s) 2122
BisI GCNGC 7 cut(s) 537, 678, 691, 1410, 2112, 2570, 2913
BlsI GCNGC 7 cut(s) 538, 679, 692, 1411, 2113, 2571, 2914
BmcAI AGTACT 3 cut(s) 337, 849, 1494
Bme1390I CCNGG 5 cut(s) 870, 1701, 1976, 2008, 2929
Bme18I GGWCC 2 cut(s) 2294, 2844
BmeRI GACNNNNNGTC 1 cut(s) 1544
BmeT110I CYCGRG 2 cut(s) 260, 1458
BmgT120I GGNCC 4 cut(s) 402, 1179, 2294, 2844
BmiI GGNNCC 8 cut(s) 119, 353, 403, 1228, 1378, 1626, 2295, 2628
BmrFI CCNGG 5 cut(s) 870, 1701, 1976, 2008, 2929
BmrI ACTGGG 1 cut(s) 613
BmsI GCATC 8 cut(s) 224, 616, 733, 967, 1684, 1711, 1980, 2275
BmuI ACTGGG 1 cut(s) 613
BpiI GAAGAC 2 cut(s) 2493, 2788
BplI GAGNNNNNCTC 2 cut(s) 891, 923
BpmI CTGGAG 2 cut(s) 1258, 2798
Bpu10I CCTNAGC 1 cut(s) 397
BpuEI CTTGAG 2 cut(s) 1758, 2079
Bsa29I ATCGAT 1 cut(s) 2241
BsaAI YACGTR 1 cut(s) 2514
BsaBI GATNNNNATC 2 cut(s) 630, 1650
BsaI GGTCTC 3 cut(s) 486, 1455, 1557
BsaJI CCNNGG 4 cut(s) 1293, 1869, 2007, 2749
BsaWI WCCGGW 1 cut(s) 1152
BsaXI ACNNNNNCTCC 4 cut(s) 210, 240, 2207, 2237
Bsc4I CCNNNNNNNGG 4 cut(s) 235, 650, 1236, 2479
Bse1I ACTGG 8 cut(s) 54, 619, 845, 1534, 1603, 1735, 1916, 2257
Bse3DI GCAATG 5 cut(s) 274, 1647, 2052, 2693, 2879
Bse8I GATNNNNATC 2 cut(s) 630, 1650
BseAI TCCGGA 1 cut(s) 1152
BseBI CCWGG 5 cut(s) 870, 1701, 1976, 2008, 2929
BseCI ATCGAT 1 cut(s) 2241
BseDI CCNNGG 4 cut(s) 1293, 1869, 2007, 2749
BseJI GATNNNNATC 2 cut(s) 630, 1650
BseLI CCNNNNNNNGG 4 cut(s) 235, 650, 1236, 2479
BseMI GCAATG 5 cut(s) 274, 1647, 2052, 2693, 2879
BseMII CTCAG 6 cut(s) 411, 1061, 1572, 2055, 2649, 2723
BseNI ACTGG 8 cut(s) 54, 619, 845, 1534, 1603, 1735, 1916, 2257
BseRI GAGGAG 2 cut(s) 912, 2228
BseSI GKGCMC 1 cut(s) 2290
BseXI GCAGC 6 cut(s) 548, 689, 1396, 2098, 2581, 2924
BsgI GTGCAG 1 cut(s) 696
Bsh1236I CGCG 1 cut(s) 1893
BshFI GGCC 5 cut(s) 5, 105, 404, 1181, 1874
BshNI GGYRCC 3 cut(s) 117, 1226, 1624
BshVI ATCGAT 1 cut(s) 2241
BsiHKAI GWGCWC 2 cut(s) 2290, 2356
BsiHKCI CYCGRG 2 cut(s) 260, 1458
BsiSI CCGG 1 cut(s) 1153
BslFI GGGAC 4 cut(s) 410, 1368, 2307, 2380
BslI CCNNNNNNNGG 4 cut(s) 235, 650, 1236, 2479
BsmAI GTCTC 5 cut(s) 244, 486, 1455, 1557, 1919
BsmFI GGGAC 4 cut(s) 410, 1368, 2307, 2380
BsnI GGCC 5 cut(s) 5, 105, 404, 1181, 1874
Bso31I GGTCTC 3 cut(s) 486, 1455, 1557
BsoBI CYCGRG 2 cut(s) 260, 1458
Bsp1286I GDGCHC 2 cut(s) 2290, 2356
Bsp13I TCCGGA 1 cut(s) 1152
BspACI CCGC 4 cut(s) 20, 690, 1505, 2402
BspANI GGCC 5 cut(s) 5, 105, 404, 1181, 1874
BspCNI CTCAG 6 cut(s) 410, 1062, 1573, 2054, 2650, 2722
BspDI ATCGAT 1 cut(s) 2241
BspEI TCCGGA 1 cut(s) 1152
BspFNI CGCG 1 cut(s) 1893
BspLI GGNNCC 8 cut(s) 119, 353, 403, 1228, 1378, 1626, 2295, 2628
BspMI ACCTGC 2 cut(s) 1612, 2776
BspPI GGATC 9 cut(s) 454, 1158, 1206, 1646, 1761, 2601, 2621, 2634, 2820
BspQI GCTCTTC 1 cut(s) 1955
BspT107I GGYRCC 3 cut(s) 117, 1226, 1624
BspTI CTTAAG 2 cut(s) 1349, 1370
BspTNI GGTCTC 3 cut(s) 486, 1455, 1557
BsrBI CCGCTC 1 cut(s) 1505
BsrDI GCAATG 5 cut(s) 274, 1647, 2052, 2693, 2879
BsrI ACTGG 8 cut(s) 54, 619, 845, 1534, 1603, 1735, 1916, 2257
BssECI CCNNGG 4 cut(s) 1293, 1869, 2007, 2749
BssNAI GTATAC 1 cut(s) 2332
Bst1107I GTATAC 1 cut(s) 2332
Bst2UI CCWGG 5 cut(s) 870, 1701, 1976, 2008, 2929
Bst6I CTCTTC 3 cut(s) 195, 1418, 1955
BstAFI CTTAAG 2 cut(s) 1349, 1370
BstAPI GCANNNNNTGC 2 cut(s) 2171, 2768
BstBAI YACGTR 1 cut(s) 2514
BstC8I GCNNGC 2 cut(s) 1389, 1845
BstDSI CCRYGG 1 cut(s) 1293
BstFNI CGCG 1 cut(s) 1893
BstMAI GTCTC 5 cut(s) 244, 486, 1455, 1557, 1919
BstMWI GCNNNNNNNGC 5 cut(s) 713, 1570, 2171, 2768, 2909
BstNI CCWGG 5 cut(s) 870, 1701, 1976, 2008, 2929
BstNSI RCATGY 5 cut(s) 257, 491, 1847, 1955, 2729
BstSCI CCNGG 5 cut(s) 868, 1699, 1974, 2006, 2927
BstSFI CTRYAG 2 cut(s) 921, 1314
BstSLI GKGCMC 1 cut(s) 2290
BstUI CGCG 1 cut(s) 1893
BstV1I GCAGC 6 cut(s) 548, 689, 1396, 2098, 2581, 2924
BstV2I GAAGAC 2 cut(s) 2493, 2788
BstX2I RGATCY 6 cut(s) 1163, 1651, 1753, 2122, 2626, 2812
BstYI RGATCY 6 cut(s) 1163, 1651, 1753, 2122, 2626, 2812
BstZ17I GTATAC 1 cut(s) 2332
Bsu15I ATCGAT 1 cut(s) 2241
BsuRI GGCC 5 cut(s) 5, 105, 404, 1181, 1874
BsuTUI ATCGAT 1 cut(s) 2241
BtgI CCRYGG 1 cut(s) 1293
BtsI GCAGTG 3 cut(s) 156, 1784, 2191
BveI ACCTGC 2 cut(s) 1612, 2776
Cac8I GCNNGC 2 cut(s) 1389, 1845
CaiI CAGNNNCTG 1 cut(s) 2768
Cfr13I GGNCC 4 cut(s) 402, 1179, 2294, 2844
ClaI ATCGAT 1 cut(s) 2241
CseI GACGC 1 cut(s) 155
CsiI ACCWGGT 1 cut(s) 1699
CspCI CAANNNNNGTGG 2 cut(s) 2064, 2099
DraIII CACNNNGTG 1 cut(s) 1703
DriI GACNNNNNGTC 1 cut(s) 1544
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 195, 1418, 1955
Eam1105I GACNNNNNGTC 1 cut(s) 1544
EarI CTCTTC 3 cut(s) 195, 1418, 1955
Eco31I GGTCTC 3 cut(s) 486, 1455, 1557
Eco32I GATATC 1 cut(s) 1882
Eco47I GGWCC 2 cut(s) 2294, 2844
Eco57I CTGAAG 7 cut(s) 219, 348, 683, 1014, 1106, 1385, 2136
Eco88I CYCGRG 2 cut(s) 260, 1458
EcoO109I RGGNCCY 4 cut(s) 402, 1179, 2294, 2844
EcoRII CCWGG 5 cut(s) 868, 1699, 1974, 2006, 2927
EcoRV GATATC 1 cut(s) 1882
EcoT22I ATGCAT 4 cut(s) 631, 1726, 1849, 2881
FalI AAGNNNNNCTT 2 cut(s) 1364, 1396
FaqI GGGAC 4 cut(s) 410, 1368, 2307, 2380
FauI CCCGC 1 cut(s) 1498
FbaI TGATCA 1 cut(s) 2389
FblI GTMKAC 2 cut(s) 1986, 2331
Fnu4HI GCNGC 7 cut(s) 537, 678, 691, 1410, 2112, 2570, 2913
Fsp4HI GCNGC 7 cut(s) 537, 678, 691, 1410, 2112, 2570, 2913
FspBI CTAG 4 cut(s) 548, 599, 750, 1016
GluI GCNGC 7 cut(s) 537, 678, 691, 1410, 2112, 2570, 2913
GsuI CTGGAG 2 cut(s) 1258, 2798
HaeIII GGCC 5 cut(s) 5, 105, 404, 1181, 1874
HapII CCGG 1 cut(s) 1153
HgaI GACGC 1 cut(s) 155
HincII GTYRAC 5 cut(s) 445, 1001, 2226, 2644, 2784
HindII GTYRAC 5 cut(s) 445, 1001, 2226, 2644, 2784
HindIII AAGCTT 6 cut(s) 772, 887, 1005, 1055, 1367, 1481
HpaII CCGG 1 cut(s) 1153
HphI GGTGA 6 cut(s) 521, 797, 952, 1088, 2445, 2884
Hpy99I CGWCG 3 cut(s) 13, 113, 2637
HpyAV CCTTC 8 cut(s) 136, 323, 365, 989, 1081, 1298, 2663, 2735
HpyCH4IV ACGT 1 cut(s) 2513
HpyF10VI GCNNNNNNNGC 5 cut(s) 713, 1570, 2171, 2768, 2909
HpySE526I ACGT 1 cut(s) 2513
Kpn2I TCCGGA 1 cut(s) 1152
KpnI GGTACC 1 cut(s) 1628
Ksp22I TGATCA 1 cut(s) 2389
LguI GCTCTTC 1 cut(s) 1955
LmnI GCTCC 1 cut(s) 264
Lsp1109I GCAGC 6 cut(s) 548, 689, 1396, 2098, 2581, 2924
LweI GCATC 8 cut(s) 224, 616, 733, 967, 1684, 1711, 1980, 2275
MabI ACCWGGT 1 cut(s) 1699
MaeI CTAG 4 cut(s) 548, 599, 750, 1016
MaeII ACGT 1 cut(s) 2513
MbiI CCGCTC 1 cut(s) 1505
MfeI CAATTG 5 cut(s) 1263, 1966, 2022, 2193, 2586
MflI RGATCY 6 cut(s) 1163, 1651, 1753, 2122, 2626, 2812
MhlI GDGCHC 2 cut(s) 2290, 2356
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 6 cut(s) 2012, 2033, 2225, 2739, 2745, 2789
MmeI TCCRAC 4 cut(s) 253, 556, 915, 1619
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 4 cut(s) 631, 1726, 1849, 2881
MroI TCCGGA 1 cut(s) 1152
MroXI GAANNNNTTC 1 cut(s) 877
MscI TGGCCA 1 cut(s) 5
MseI TTAA 8 cut(s) 419, 792, 1350, 1371, 1382, 1430, 1743, 2343
MslI CAYNNNNRTG 6 cut(s) 672, 731, 914, 1029, 2283, 2361
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 539, 2657
MspCI CTTAAG 2 cut(s) 1349, 1370
MspI CCGG 1 cut(s) 1153
MspR9I CCNGG 5 cut(s) 870, 1701, 1976, 2008, 2929
MunI CAATTG 5 cut(s) 1263, 1966, 2022, 2193, 2586
MvaI CCWGG 5 cut(s) 870, 1701, 1976, 2008, 2929
MvnI CGCG 1 cut(s) 1893
MwoI GCNNNNNNNGC 5 cut(s) 713, 1570, 2171, 2768, 2909
NlaIV GGNNCC 8 cut(s) 119, 353, 403, 1228, 1378, 1626, 2295, 2628
NmuCI GTSAC 9 cut(s) 722, 733, 901, 916, 1061, 2062, 2485, 2536, 2719
NsiI ATGCAT 4 cut(s) 631, 1726, 1849, 2881
NspI RCATGY 5 cut(s) 257, 491, 1847, 1955, 2729
OliI CACNNNNGTG 1 cut(s) 672
PaeI GCATGC 1 cut(s) 1847
PciI ACATGT 2 cut(s) 253, 1951
PciSI GCTCTTC 1 cut(s) 1955
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdmI GAANNNNTTC 1 cut(s) 877
PfeI GAWTC 7 cut(s) 830, 1039, 1799, 2149, 2428, 2690, 2852
PflMI CCANNNNNTGG 1 cut(s) 1236
PfoI TCCNGGA 1 cut(s) 1974
PkrI GCNGC 7 cut(s) 538, 679, 692, 1411, 2113, 2571, 2914
PleI GAGTC 6 cut(s) 2011, 2033, 2224, 2739, 2744, 2788
PpsI GAGTC 6 cut(s) 2011, 2033, 2224, 2739, 2744, 2788
Ppu21I YACGTR 1 cut(s) 2514
PpuMI RGGWCCY 2 cut(s) 2294, 2844
PscI ACATGT 2 cut(s) 253, 1951
PshBI ATTAAT 1 cut(s) 1743
PsiI TTATAA 2 cut(s) 770, 1139
Psp5II RGGWCCY 2 cut(s) 2294, 2844
Psp6I CCWGG 5 cut(s) 868, 1699, 1974, 2006, 2927
PspGI CCWGG 5 cut(s) 868, 1699, 1974, 2006, 2927
PspN4I GGNNCC 8 cut(s) 119, 353, 403, 1228, 1378, 1626, 2295, 2628
PspPI GGNCC 4 cut(s) 402, 1179, 2294, 2844
PspPPI RGGWCCY 2 cut(s) 2294, 2844
PstNI CAGNNNCTG 1 cut(s) 2768
PsuI RGATCY 6 cut(s) 1163, 1651, 1753, 2122, 2626, 2812
PvuII CAGCTG 2 cut(s) 539, 2657
RseI CAYNNNNRTG 6 cut(s) 672, 731, 914, 1029, 2283, 2361
SapI GCTCTTC 1 cut(s) 1955
SaqAI TTAA 8 cut(s) 419, 792, 1350, 1371, 1382, 1430, 1743, 2343
SatI GCNGC 7 cut(s) 537, 678, 691, 1410, 2112, 2570, 2913
Sau96I GGNCC 4 cut(s) 402, 1179, 2294, 2844
ScaI AGTACT 3 cut(s) 337, 849, 1494
SchI GAGTC 6 cut(s) 2012, 2033, 2225, 2739, 2745, 2789
ScrFI CCNGG 5 cut(s) 870, 1701, 1976, 2008, 2929
SduI GDGCHC 2 cut(s) 2290, 2356
SexAI ACCWGGT 1 cut(s) 1699
SfaNI GCATC 8 cut(s) 224, 616, 733, 967, 1684, 1711, 1980, 2275
SfcI CTRYAG 2 cut(s) 921, 1314
SinI GGWCC 2 cut(s) 2294, 2844
SmiMI CAYNNNNRTG 6 cut(s) 672, 731, 914, 1029, 2283, 2361
SmlI CTYRAG 4 cut(s) 1349, 1370, 1773, 2094
SmoI CTYRAG 4 cut(s) 1349, 1370, 1773, 2094
SpeI ACTAGT 1 cut(s) 1015
SphI GCATGC 1 cut(s) 1847
SsiI CCGC 4 cut(s) 20, 690, 1505, 2402
SspI AATATT 2 cut(s) 711, 1609
SspMI CTAG 4 cut(s) 548, 599, 750, 1016
StyD4I CCNGG 5 cut(s) 868, 1699, 1974, 2006, 2927
TaiI ACGT 1 cut(s) 2516
TaqI TCGA 8 cut(s) 111, 279, 719, 1675, 2001, 2241, 2635, 2739
TatI WGTACW 5 cut(s) 335, 595, 847, 1273, 1492
TauI GCSGC 1 cut(s) 693
TfiI GAWTC 7 cut(s) 830, 1039, 1799, 2149, 2428, 2690, 2852
Tru1I TTAA 8 cut(s) 419, 792, 1350, 1371, 1382, 1430, 1743, 2343
Tru9I TTAA 8 cut(s) 419, 792, 1350, 1371, 1382, 1430, 1743, 2343
TseFI GTSAC 9 cut(s) 722, 733, 901, 916, 1061, 2062, 2485, 2536, 2719
TseI GCWGC 6 cut(s) 536, 677, 1409, 2111, 2569, 2912
Tsp45I GTSAC 9 cut(s) 722, 733, 901, 916, 1061, 2062, 2485, 2536, 2719
TspDTI ATGAA 6 cut(s) 146, 1031, 1662, 1808, 2454, 2682
TspGWI ACGGA 2 cut(s) 666, 871
Van91I CCANNNNNTGG 1 cut(s) 1236
Vha464I CTTAAG 2 cut(s) 1349, 1370
VneI GTGCAC 1 cut(s) 2286
VpaK11BI GGWCC 2 cut(s) 2294, 2844
VspI ATTAAT 1 cut(s) 1743
XapI RAATTY 2 cut(s) 433, 454
XbaI TCTAGA 1 cut(s) 547
XceI RCATGY 5 cut(s) 257, 491, 1847, 1955, 2729
XcmI CCANNNNNNNNNTGG 2 cut(s) 13, 2217
XmiI GTMKAC 2 cut(s) 1986, 2331
XmnI GAANNNNTTC 1 cut(s) 877
XspI CTAG 4 cut(s) 548, 599, 750, 1016
ZrmI AGTACT 3 cut(s) 337, 849, 1494
Zsp2I ATGCAT 4 cut(s) 631, 1726, 1849, 2881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.