Rroxscaffold_7G00162180

ER membrane protein complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011093
Physical Location & Seq
Forward (+)
4792937 .. 4799793
6857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00162180.1

Sequence Viewer

Length: 3900 bp
ATGGCCACGATGAGCATGGCGGTTAGGGCTTTTCTTCTTCTTCTTCTACTGGTATTCCTATCTCTCGCAAATCTCACTCTTTCTCTTTACGAAGATCAAGTCGGCCTCGTCGATTGGCACCAGCAGTACATAGGGAAAGTGAAGGACGCAGTGTTTCATACTCAAAAGAGTGGGCGAAAGCGTGTTGTGGTGTCCACTGAAGAGAATGTTATAGCATCACTTGATCTCCGACACGGGGAGATATTTTGGAGACATGTTCTTGGGAGCAATGATGTTGTCGATGGAATTGACATAGCACTGGGAAAATTTGTCATTACCCTTTCGTCTGAAGGAAGTATTTTGAGAGCATGGAACCTTCCAGATGGTCAGATGGTGTGGCAATCTTTTATAGACGGCTCAGGGGCCTCAAAATCATTATTAACTGTCCCGACAAATTTGATCGTCAACAAGGAAAAGTTGATCCTTGTTTTTGGTAAAGGGTCTCTACATGCTGTTTCTGGCATTGATGGTGAGGTTCTTTGGACAAAGGATTTTGCAGCTGAAAGTCTAGAGGTTCAACATATTATTCAACCTGTTGGAAGTGAGGCAATCTATGTACTAGGATTTGTTGGTTCTTCCCAGTTTGATGCATATCAGATCAATCCTAAGAACGGAGAGATACTGAAGCACAATAGTGCAGCACTTTCTGGCGGCTATTCTGGGGAAGCAATATTGGCTTCGAGTGACATTCTTGTGACATTGGATGCCTCTAGGTCAAAGTTGGTAGTTTTAAGCTTTCAGGATGGTGAACTTAACTTGCAACAGACATCCATATCTGATATATTGGGGGATTCTTCTGGAACACCAGTCCTGTTATCTTCAAAACTACCAGGAATGTTTTCCATAAAGCTTGATGGAGGAGTGACACTCATAAAAGTGACTGTAGAAGCCAAGTTGGAGGTGATGGATAAAATAAGTAGTGTGGCAGCTGTTAGTGATGCAATTCCCCTTACTGAAGGTCAACAAGCTTTCGCACTAGTTCAACATGGAGATGGTAAGATTCATCTTACAGTGAAGCTTAGTCACGATCTGAGTGGTGATTTGCTGAAGGAGAGTATAGTTATGGACAATCAGAGGGGGATGGTGCATAAGGTTTTTATAAACAGTTATATACGGACAGACAGATCCAATGGGTTTAGGGCCTTGATTGTCATGGAGGATCATTCACTTTTGTTGTTACAACAAGGTGCCATTGTCTGGAGTAGGGAGGATGGTCTTGCCTCAATTGTTGATGTACTAACATCAGAACTACCCGTGGAAAAGGAAGGTGTATCTGTAGCAAGAGTTGAGGAAAACCTTTTTGAGTGGCTTAAGGGACACCTACTGAAGCTTAAGGGAACCTTAATGCTTGCAAGTGCTGATGATGTTGCTGCTATACAAGAGAGGAGGTTAAAAAGTTCTGAAAAGAGCAAACTGACCCGAGACCATAATGGTTTTCGGAAGCTTCTCATAGTACTTACGAGAGCGGGAAAGCTCTTTGCCTTGCACACTGGATATGGACAAGTTGTCTGGTCTCTGTTACTGCCTAATCTGCGTAAATCTGAGTGCAAGTTTCCGACTGGTTTGAATATTTATCAGTGGCAGGTACCCCATCATCACGCAATGGATGAAGATCCATCTATCCTCATAGTAGGTCAATGTGGACAAAGTTCTGATGCACCAGGTGTTCTGTCTATTGTTGATGCATATACTGGCACTGAAATTAATTCAATGGATCTCGTACATTCCATTTCGCAAGTTATTCCACTGCCATTTACTGATTCAACAGAACAGCGACTTCATCTACTAATAGATGGAAACCAGCATGCATATCTATATCCAAGAACATCCGAGGCCATTGATATCTTTCAACGCGAGTTCTCAAACATATACTGGTACTCAGTTGAGACTAAGAATGGCATTATCAAAGGACATGTCTTGAAGAGCAATTGCATCCAGGAAGTTGTAGACAGCTATTGCTTCGAGTCCAGGGATATATGGTCAATTGTATTCCCATCTGACTCAGAAAAGATCATTGCAACAGTGACAAGAAAACCAAATGAGGTGGTTCACACTCAAGCAAAGGTTATAGCTGCTGAAGAAGATCTGATGTATAAGTATATATCTAAGAATCTACTCTTTGTTGCAACTGTTGCACCTAAAGGCAGTGGAGCAATTGGAACAGCTACCCCAGAGGAGTCGTGGTTGACTGTATATCTTATCGATACTGTAACTGGTCGTATATTGCATCGGATGACCCATCATGGTGCACAGGGACCTGTCCACGCTGTTTTCAGTGAGAATTGGGTTGTATACCACTACTTTAATCTTAGAGCACACAGATATGAGATGTCAGTCATAGAGATATATGATCAGTCCCGAGCGGACAATAAGGATGTTTGGAAGCTGATTCTTGGGAAACATAATCTCACTTCACCTATTTCTTCATATTCCCGACCAGAGGTTGTGACGAAATCACAGTCTTACTTTTTCACGTATTCTGTAAAAGCAATAGATGTGACATTGACAGCAAAGGGTATTACTTCAAAGCAGCTTCTTATTGGTACAATTGGGGATCAGGTTTTGGCACTGGACAAGCGATTTTTGGATCCCCGTCGATCAGTCAACCCTACACCAGCTGAGAAAGAGGAAGGCATTATACCTCTAACAGATTCATTGCCAATAATTCCTCAGTCCTATGTGACACATGCTCTAAGAGTCGAAGGACTCCGAGGCATAGTGACAGTACCTGCCAAGCTGGAGTCAACAACCCTTGTCTTCGTATATGGAGTGGATCTGTTTTTTACTCAACTCGCACCATCAAGGACCTACGATTCGCTGACTGATGATTTCAGCTATGCATTGCTTCTCATCACCATCGTTGCACTCATAGCAGCAATCTTTGTAACCTGGATTTTATCCGAGAAAAAAGAACTACGAGAGAAGTGGAGTTCTCAGCCCCACCACGCCTCATTCTCCAACTCTCCCTTTCTCATCCACCGATCCACTACCACCTTCGTTGTCAATTCCCGCTCCTTCGACACAATCCGACCCCTAACCCACCACCAGTCGAAGCCCGACTCCGAATCATCTTCCTCCTCAACCTCACAGAGCTTCACCCAGTGGAAATTCCCTCTCCCTGAGTCACCCATTCTCCCCCACCCACAAGCTGGATCGGACCAGGATTTTGACGCTGTGGCCATATTTCAGTCCAGCCCAGCACCGCCGCCGCCGCCGCCCATGTCCTCCACCGGCCTCCAAGAGCTCTTCCATGCAGCAGAGCTCCAACTCAGCATCGGGTCATTTCCGGAGCAGCTCGTAGCACTCCAGCTCCTGGAACGCTCTCTGGTCCCCTACCCGCCATCCGACCCGGAGTGCCCGCCCGAATTGATGCGCGGGTTGGTGCGAAATTTGAAAAACAAAGCGGGGGCAAAACCGGCAACGAAAGTGTTGTTGGCGCTCTGTTTGGCGGAGGCTAACCGCCACGTGGCGGTTGAGGCCGGGGCAGCTTCTGCGGTGGTCGAGACGGCGCTGGAGCTGGAGGATACGGCGGCAGAGCGGGCCCTGGCGGCTCTGGAGTTAATGTGCACTGTGGCGGAGGGGGCGGCGGAGGTGAGGTCCCACGCGCTGGCGGTGCCGGTGATGGTGATGATGATGGGCAGGACGTCGGCTCGGGGTAAGGAATACGCAATAGGGGTGTTGGCGGTTATATACGGTGGCGCATCTGAAGATGGGGCCGCGGAAGCGGTTTCGGCAGCTCCGGCGGAGGAGGTGGCCCGTGCAGTGGAGTTGGCGTTGAAAGGGGATTGTAGTGGTAGGGGAAGGAGGAAAGGGGCCCAATTATTGAAGGCGCTTCAACCAGAAGAAGAGAAAGAGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1620 PQQ_2

Protein Analysis

1299

Amino Acids

142.27

Weight (kDa)

5.82

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EMC1_N PF25293 29 - 417 3.1e-136 EMC1 N-terminal beta-propeller domain
PQQ_2 PF13360 56 - 263 2.9e-09 Outer membrane protein assembly factor BamB
EMC1_C PF07774 772 - 989 4.2e-68 ER membrane protein complex subunit 1, C-terminal
ARM_PUB PF25598 1136 - 1292 1e-21 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11560
fragaria_vesca FvH4_2g35850
malus_domestica MD08G1115900.v1.1 MD15G1095600.v1.1
prunus_persica Prupe.1G450600_v2.0.a1 Prupe.1G450600_v2.0.a1
pyrus_communis pycom08g09670 pycom08g09680 pycom15g08790
rosa_chinensis RchiOBHm_Chr6g0305971
rosa_laevigata RLG00000010841
rosa_multiflora Rmu_co8311943.1_g000001 Rmu_sc0001809.1_g000056
rosa_roxburghii Rroxscaffold_7G00162180
rosa_rugosa Rorug06G0345700
rosa_samantha Rh6AG457600 Rh6BG443500 Rh6CG471700 Rh6DG458100
rosa_wichuraiana Rw6G039920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1139
AatII GACGTC 1 cut(s) 3686
Acc36I ACCTGC 2 cut(s) 1612, 2776
Acc65I GGTACC 1 cut(s) 1624
AccB1I GGYRCC 4 cut(s) 117, 1226, 1624, 3652
AccB7I CCANNNNNTGG 4 cut(s) 1236, 3188, 3352, 3646
AccBSI CCGCTC 4 cut(s) 1505, 2402, 3051, 3577
AccI GTMKAC 2 cut(s) 1986, 2331
AccII CGCG 4 cut(s) 1893, 3414, 3644, 3758
AccIII TCCGGA 1 cut(s) 3325
AcoI YGGCCR 2 cut(s) 3, 3216
AcsI RAATTY 4 cut(s) 305, 433, 3146, 3427
AcuI CTGAAG 8 cut(s) 219, 348, 683, 1014, 1106, 1385, 2136, 3765
AcvI CACGTG 1 cut(s) 3505
AcyI GRCGYC 1 cut(s) 3683
AdeI CACNNNGTG 2 cut(s) 1703, 3141
AfaI GTAC 9 cut(s) 128, 597, 1275, 1494, 1626, 1761, 1916, 2584, 2766
AflII CTTAAG 2 cut(s) 1349, 1370
AflIII ACRYGT 2 cut(s) 253, 1951
AhdI GACNNNNNGTC 1 cut(s) 1544
AhlI ACTAGT 1 cut(s) 1015
AjnI CCWGG 8 cut(s) 868, 1699, 1974, 2006, 2927, 3198, 3351, 3582
AjuI GAANNNNNNNTTGG 2 cut(s) 3455, 3487
AleI CACNNNNGTG 1 cut(s) 672
Alw21I GWGCWC 5 cut(s) 2290, 2356, 3285, 3303, 3608
Alw26I GTCTC 7 cut(s) 244, 486, 1455, 1557, 1919, 3536, 3887
Alw44I GTGCAC 2 cut(s) 2286, 3604
AlwNI CAGNNNCTG 3 cut(s) 2768, 3352, 3530
Ama87I CYCGRG 3 cut(s) 1458, 2397, 3690
Aor13HI TCCGGA 1 cut(s) 3325
ApaI GGGCCC 2 cut(s) 3583, 3856
ApaLI GTGCAC 2 cut(s) 2286, 3604
ApoI RAATTY 4 cut(s) 305, 433, 3146, 3427
AseI ATTAAT 1 cut(s) 1743
Asp700I GAANNNNTTC 1 cut(s) 877
Asp718I GGTACC 1 cut(s) 1624
AspLEI GCGC 6 cut(s) 3414, 3478, 3550, 3646, 3740, 3871
AsuC2I CCSGG 2 cut(s) 3389, 3520
AvaI CYCGRG 3 cut(s) 1458, 2397, 3690
AvaII GGWCC 5 cut(s) 2294, 2844, 3196, 3367, 3636
BaeGI GKGCMC 5 cut(s) 2290, 3398, 3583, 3608, 3856
BalI TGGCCA 2 cut(s) 5, 3218
BamHI GGATCC 1 cut(s) 2626
BanI GGYRCC 4 cut(s) 117, 1226, 1624, 3652
BanII GRGCYC 4 cut(s) 3285, 3303, 3583, 3856
BarI GAAGNNNNNNTAC 2 cut(s) 2661, 2693
BbrPI CACGTG 1 cut(s) 3505
BbsI GAAGAC 1 cut(s) 2788
Bbv12I GWGCWC 5 cut(s) 2290, 2356, 3285, 3303, 3608
BceAI ACGGC 3 cut(s) 409, 3561, 3582
BciT130I CCWGG 8 cut(s) 870, 1701, 1976, 2008, 2929, 3200, 3353, 3584
BciVI GTATCC 1 cut(s) 3556
BclI TGATCA 1 cut(s) 2389
BcnI CCSGG 2 cut(s) 3389, 3520
BcoDI GTCTC 7 cut(s) 244, 486, 1455, 1557, 1919, 3536, 3887
BcuI ACTAGT 1 cut(s) 1015
BfaI CTAG 4 cut(s) 548, 599, 750, 1016
BfmI CTRYAG 2 cut(s) 921, 1314
BfoI RGCGCY 3 cut(s) 3479, 3551, 3872
BfrI CTTAAG 2 cut(s) 1349, 1370
BfuAI ACCTGC 2 cut(s) 1612, 2776
BfuI GTATCC 1 cut(s) 3556
BglI GCCNNNNNGGC 1 cut(s) 3587
BglII AGATCT 1 cut(s) 2122
BmcAI AGTACT 1 cut(s) 1494
Bme18I GGWCC 5 cut(s) 2294, 2844, 3196, 3367, 3636
BmeRI GACNNNNNGTC 1 cut(s) 1544
BmeT110I CYCGRG 3 cut(s) 1458, 2397, 3690
BmrI ACTGGG 3 cut(s) 308, 613, 3133
BmuI ACTGGG 3 cut(s) 308, 613, 3133
BpiI GAAGAC 1 cut(s) 2788
BplI GAGNNNNNCTC 2 cut(s) 891, 923
BpmI CTGGAG 6 cut(s) 1258, 2798, 3329, 3572, 3578, 3614
Bpu10I CCTNAGC 1 cut(s) 397
BpuEI CTTGAG 1 cut(s) 2079
BpuMI CCSGG 2 cut(s) 3389, 3520
Bsa29I ATCGAT 1 cut(s) 2241
BsaAI YACGTR 2 cut(s) 2514, 3505
BsaBI GATNNNNATC 2 cut(s) 630, 1650
BsaHI GRCGYC 1 cut(s) 3683
BsaI GGTCTC 4 cut(s) 486, 1455, 1557, 3887
BsaJI CCNNGG 7 cut(s) 1293, 1869, 2007, 2749, 3519, 3582, 3756
BsaWI WCCGGW 1 cut(s) 3325
Bse118I RCCGGY 3 cut(s) 3269, 3454, 3655
Bse3DI GCAATG 5 cut(s) 274, 1647, 2052, 2693, 2879
Bse8I GATNNNNATC 2 cut(s) 630, 1650
BseAI TCCGGA 1 cut(s) 3325
BseBI CCWGG 8 cut(s) 870, 1701, 1976, 2008, 2929, 3200, 3353, 3584
BseCI ATCGAT 1 cut(s) 2241
BseDI CCNNGG 7 cut(s) 1293, 1869, 2007, 2749, 3519, 3582, 3756
BseJI GATNNNNATC 2 cut(s) 630, 1650
BseMI GCAATG 5 cut(s) 274, 1647, 2052, 2693, 2879
BseRI GAGGAG 5 cut(s) 912, 1438, 2228, 3106, 3800
BseSI GKGCMC 5 cut(s) 2290, 3398, 3583, 3608, 3856
BseYI CCCAGC 1 cut(s) 3235
BsgI GTGCAG 2 cut(s) 696, 3819
Bsh1236I CGCG 4 cut(s) 1893, 3414, 3644, 3758
BshNI GGYRCC 4 cut(s) 117, 1226, 1624, 3652
BshVI ATCGAT 1 cut(s) 2241
BsiHKAI GWGCWC 5 cut(s) 2290, 2356, 3285, 3303, 3608
BsiHKCI CYCGRG 3 cut(s) 1458, 2397, 3690
BsiSI CCGG 7 cut(s) 3270, 3326, 3389, 3455, 3519, 3656, 3779
BslFI GGGAC 6 cut(s) 410, 1368, 2307, 2380, 3353, 3622
BsmAI GTCTC 7 cut(s) 244, 486, 1455, 1557, 1919, 3536, 3887
BsmBI CGTCTC 1 cut(s) 3536
BsmFI GGGAC 6 cut(s) 410, 1368, 2307, 2380, 3353, 3622
Bso31I GGTCTC 4 cut(s) 486, 1455, 1557, 3887
BsoBI CYCGRG 3 cut(s) 1458, 2397, 3690
Bsp120I GGGCCC 2 cut(s) 3579, 3852
Bsp1286I GDGCHC 8 cut(s) 2290, 2356, 3285, 3303, 3398, 3583, 3608, 3856
Bsp13I TCCGGA 1 cut(s) 3325
BspDI ATCGAT 1 cut(s) 2241
BspEI TCCGGA 1 cut(s) 3325
BspFNI CGCG 4 cut(s) 1893, 3414, 3644, 3758
BspMI ACCTGC 2 cut(s) 1612, 2776
BspQI GCTCTTC 2 cut(s) 1955, 3290
BspT107I GGYRCC 4 cut(s) 117, 1226, 1624, 3652
BspTI CTTAAG 2 cut(s) 1349, 1370
BspTNI GGTCTC 4 cut(s) 486, 1455, 1557, 3887
BsrBI CCGCTC 4 cut(s) 1505, 2402, 3051, 3577
BsrDI GCAATG 5 cut(s) 274, 1647, 2052, 2693, 2879
BsrFI RCCGGY 3 cut(s) 3269, 3454, 3655
BssAI RCCGGY 3 cut(s) 3269, 3454, 3655
BssECI CCNNGG 7 cut(s) 1293, 1869, 2007, 2749, 3519, 3582, 3756
BssNAI GTATAC 1 cut(s) 2332
BssNI GRCGYC 1 cut(s) 3683
Bst1107I GTATAC 1 cut(s) 2332
Bst2UI CCWGG 8 cut(s) 870, 1701, 1976, 2008, 2929, 3200, 3353, 3584
Bst6I CTCTTC 4 cut(s) 195, 1955, 3290, 3879
BstACI GRCGYC 1 cut(s) 3683
BstAFI CTTAAG 2 cut(s) 1349, 1370
BstAPI GCANNNNNTGC 2 cut(s) 2171, 3530
BstBAI YACGTR 2 cut(s) 2514, 3505
BstC8I GCNNGC 5 cut(s) 1389, 1845, 3398, 3579, 3648
BstDSI CCRYGG 2 cut(s) 1293, 3756
BstFNI CGCG 4 cut(s) 1893, 3414, 3644, 3758
BstH2I RGCGCY 3 cut(s) 3479, 3551, 3872
BstHHI GCGC 6 cut(s) 3414, 3478, 3550, 3646, 3740, 3871
BstMAI GTCTC 7 cut(s) 244, 486, 1455, 1557, 1919, 3536, 3887
BstNI CCWGG 8 cut(s) 870, 1701, 1976, 2008, 2929, 3200, 3353, 3584
BstNSI RCATGY 5 cut(s) 257, 491, 1847, 1955, 2729
BstSFI CTRYAG 2 cut(s) 921, 1314
BstSLI GKGCMC 5 cut(s) 2290, 3398, 3583, 3608, 3856
BstUI CGCG 4 cut(s) 1893, 3414, 3644, 3758
BstV2I GAAGAC 1 cut(s) 2788
BstX2I RGATCY 6 cut(s) 1163, 1651, 1753, 2122, 2626, 2812
BstYI RGATCY 6 cut(s) 1163, 1651, 1753, 2122, 2626, 2812
BstZ17I GTATAC 1 cut(s) 2332
Bsu15I ATCGAT 1 cut(s) 2241
BsuI GTATCC 1 cut(s) 3556
BsuTUI ATCGAT 1 cut(s) 2241
BtgI CCRYGG 2 cut(s) 1293, 3756
BtsI GCAGTG 4 cut(s) 156, 1784, 2191, 3807
BveI ACCTGC 2 cut(s) 1612, 2776
Cac8I GCNNGC 5 cut(s) 1389, 1845, 3398, 3579, 3648
CaiI CAGNNNCTG 3 cut(s) 2768, 3352, 3530
CfoI GCGC 6 cut(s) 3414, 3478, 3550, 3646, 3740, 3871
Cfr10I RCCGGY 3 cut(s) 3269, 3454, 3655
Cfr42I CCGCGG 1 cut(s) 3759
ClaI ATCGAT 1 cut(s) 2241
CseI GACGC 2 cut(s) 155, 3218
CsiI ACCWGGT 1 cut(s) 1699
Csp6I GTAC 9 cut(s) 127, 596, 1274, 1493, 1625, 1760, 1915, 2583, 2765
CspCI CAANNNNNGTGG 4 cut(s) 2064, 2099, 3019, 3054
CviQI GTAC 9 cut(s) 127, 596, 1274, 1493, 1625, 1760, 1915, 2583, 2765
DraIII CACNNNGTG 2 cut(s) 1703, 3141
DriI GACNNNNNGTC 1 cut(s) 1544
EaeI YGGCCR 2 cut(s) 3, 3216
Eam1104I CTCTTC 4 cut(s) 195, 1955, 3290, 3879
Eam1105I GACNNNNNGTC 1 cut(s) 1544
EarI CTCTTC 4 cut(s) 195, 1955, 3290, 3879
EciI GGCGGA 4 cut(s) 3503, 3629, 3641, 3797
Ecl136II GAGCTC 2 cut(s) 3283, 3301
Eco24I GRGCYC 4 cut(s) 3285, 3303, 3583, 3856
Eco31I GGTCTC 4 cut(s) 486, 1455, 1557, 3887
Eco32I GATATC 1 cut(s) 1882
Eco47I GGWCC 5 cut(s) 2294, 2844, 3196, 3367, 3636
Eco53kI GAGCTC 2 cut(s) 3283, 3301
Eco57I CTGAAG 8 cut(s) 219, 348, 683, 1014, 1106, 1385, 2136, 3765
Eco72I CACGTG 1 cut(s) 3505
Eco88I CYCGRG 3 cut(s) 1458, 2397, 3690
EcoICRI GAGCTC 2 cut(s) 3283, 3301
EcoO109I RGGNCCY 7 cut(s) 402, 1179, 2294, 2844, 3580, 3636, 3852
EcoRII CCWGG 8 cut(s) 868, 1699, 1974, 2006, 2927, 3198, 3351, 3582
EcoRV GATATC 1 cut(s) 1882
EcoT22I ATGCAT 4 cut(s) 631, 1726, 1849, 2881
EcoT38I GRGCYC 4 cut(s) 3285, 3303, 3583, 3856
Esp3I CGTCTC 1 cut(s) 3536
FalI AAGNNNNNCTT 2 cut(s) 1364, 1396
FaqI GGGAC 6 cut(s) 410, 1368, 2307, 2380, 3353, 3622
FauI CCCGC 7 cut(s) 1498, 3056, 3384, 3405, 3407, 3436, 3570
FbaI TGATCA 1 cut(s) 2389
FblI GTMKAC 2 cut(s) 1986, 2331
FriOI GRGCYC 4 cut(s) 3285, 3303, 3583, 3856
FspBI CTAG 4 cut(s) 548, 599, 750, 1016
GlaI GCGC 6 cut(s) 3413, 3477, 3549, 3645, 3739, 3870
GsaI CCCAGC 1 cut(s) 3239
GsuI CTGGAG 6 cut(s) 1258, 2798, 3329, 3572, 3578, 3614
HaeII RGCGCY 3 cut(s) 3479, 3551, 3872
HapII CCGG 7 cut(s) 3270, 3326, 3389, 3455, 3519, 3656, 3779
HgaI GACGC 2 cut(s) 155, 3218
HhaI GCGC 6 cut(s) 3414, 3478, 3550, 3646, 3740, 3871
Hin1I GRCGYC 1 cut(s) 3683
Hin6I GCGC 6 cut(s) 3412, 3476, 3548, 3644, 3738, 3869
HinP1I GCGC 6 cut(s) 3412, 3476, 3548, 3644, 3738, 3869
HincII GTYRAC 5 cut(s) 445, 1001, 2226, 2644, 2784
HindII GTYRAC 5 cut(s) 445, 1001, 2226, 2644, 2784
HindIII AAGCTT 6 cut(s) 772, 887, 1005, 1055, 1367, 1481
HpaII CCGG 7 cut(s) 3270, 3326, 3389, 3455, 3519, 3656, 3779
Hpy99I CGWCG 3 cut(s) 113, 2637, 3688
HpyCH4IV ACGT 3 cut(s) 2513, 3504, 3683
HpySE526I ACGT 3 cut(s) 2513, 3504, 3683
Hsp92I GRCGYC 1 cut(s) 3683
HspAI GCGC 6 cut(s) 3412, 3476, 3548, 3644, 3738, 3869
Kpn2I TCCGGA 1 cut(s) 3325
KpnI GGTACC 1 cut(s) 1628
Ksp22I TGATCA 1 cut(s) 2389
KspI CCGCGG 1 cut(s) 3759
LguI GCTCTTC 2 cut(s) 1955, 3290
LmnI GCTCC 8 cut(s) 264, 2189, 3056, 3306, 3328, 3354, 3553, 3781
MabI ACCWGGT 1 cut(s) 1699
MaeI CTAG 4 cut(s) 548, 599, 750, 1016
MaeII ACGT 3 cut(s) 2513, 3504, 3683
MbiI CCGCTC 4 cut(s) 1505, 2402, 3051, 3577
MfeI CAATTG 5 cut(s) 1263, 1966, 2022, 2193, 2586
MflI RGATCY 6 cut(s) 1163, 1651, 1753, 2122, 2626, 2812
MhlI GDGCHC 8 cut(s) 2290, 2356, 3285, 3303, 3398, 3583, 3608, 3856
MlsI TGGCCA 2 cut(s) 5, 3218
MluNI TGGCCA 2 cut(s) 5, 3218
MlyI GAGTC 8 cut(s) 2012, 2033, 2225, 2739, 2745, 2789, 3092, 3170
MmeI TCCRAC 8 cut(s) 253, 556, 915, 1619, 3021, 3091, 3328, 3408
Mox20I TGGCCA 2 cut(s) 5, 3218
Mph1103I ATGCAT 4 cut(s) 631, 1726, 1849, 2881
MroI TCCGGA 1 cut(s) 3325
MroXI GAANNNNTTC 1 cut(s) 877
MscI TGGCCA 2 cut(s) 5, 3218
MslI CAYNNNNRTG 6 cut(s) 672, 731, 914, 1029, 2283, 2361
Msp20I TGGCCA 2 cut(s) 5, 3218
MspA1I CMGCKG 4 cut(s) 539, 968, 2657, 3758
MspCI CTTAAG 2 cut(s) 1349, 1370
MspI CCGG 7 cut(s) 3270, 3326, 3389, 3455, 3519, 3656, 3779
MunI CAATTG 5 cut(s) 1263, 1966, 2022, 2193, 2586
MvaI CCWGG 8 cut(s) 870, 1701, 1976, 2008, 2929, 3200, 3353, 3584
MvnI CGCG 4 cut(s) 1893, 3414, 3644, 3758
NciI CCSGG 2 cut(s) 3389, 3520
NsiI ATGCAT 4 cut(s) 631, 1726, 1849, 2881
NspI RCATGY 5 cut(s) 257, 491, 1847, 1955, 2729
OliI CACNNNNGTG 1 cut(s) 672
PaeI GCATGC 1 cut(s) 1847
PciI ACATGT 2 cut(s) 253, 1951
PciSI GCTCTTC 2 cut(s) 1955, 3290
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdmI GAANNNNTTC 1 cut(s) 877
PfeI GAWTC 8 cut(s) 830, 1039, 1799, 2149, 2428, 2690, 2852, 3104
PflMI CCANNNNNTGG 4 cut(s) 1236, 3188, 3352, 3646
PfoI TCCNGGA 2 cut(s) 1974, 3351
PleI GAGTC 8 cut(s) 2011, 2033, 2224, 2739, 2744, 2788, 3092, 3169
PmaCI CACGTG 1 cut(s) 3505
PmlI CACGTG 1 cut(s) 3505
PpsI GAGTC 8 cut(s) 2011, 2033, 2224, 2739, 2744, 2788, 3092, 3169
Ppu21I YACGTR 2 cut(s) 2514, 3505
PpuMI RGGWCCY 3 cut(s) 2294, 2844, 3636
PscI ACATGT 2 cut(s) 253, 1951
PshBI ATTAAT 1 cut(s) 1743
PsiI TTATAA 1 cut(s) 1139
Psp124BI GAGCTC 2 cut(s) 3285, 3303
Psp5II RGGWCCY 3 cut(s) 2294, 2844, 3636
Psp6I CCWGG 8 cut(s) 868, 1699, 1974, 2006, 2927, 3198, 3351, 3582
PspCI CACGTG 1 cut(s) 3505
PspFI CCCAGC 1 cut(s) 3235
PspGI CCWGG 8 cut(s) 868, 1699, 1974, 2006, 2927, 3198, 3351, 3582
PspOMI GGGCCC 2 cut(s) 3579, 3852
PspPPI RGGWCCY 3 cut(s) 2294, 2844, 3636
PstNI CAGNNNCTG 3 cut(s) 2768, 3352, 3530
PsuI RGATCY 6 cut(s) 1163, 1651, 1753, 2122, 2626, 2812
PvuII CAGCTG 3 cut(s) 539, 968, 2657
RsaI GTAC 9 cut(s) 128, 597, 1275, 1494, 1626, 1761, 1916, 2584, 2766
RsaNI GTAC 9 cut(s) 127, 596, 1274, 1493, 1625, 1760, 1915, 2583, 2765
RseI CAYNNNNRTG 6 cut(s) 672, 731, 914, 1029, 2283, 2361
SacI GAGCTC 2 cut(s) 3285, 3303
SacII CCGCGG 1 cut(s) 3759
SapI GCTCTTC 2 cut(s) 1955, 3290
ScaI AGTACT 1 cut(s) 1494
SchI GAGTC 8 cut(s) 2012, 2033, 2225, 2739, 2745, 2789, 3092, 3170
SduI GDGCHC 8 cut(s) 2290, 2356, 3285, 3303, 3398, 3583, 3608, 3856
SexAI ACCWGGT 1 cut(s) 1699
SfcI CTRYAG 2 cut(s) 921, 1314
Sfr303I CCGCGG 1 cut(s) 3759
SgrBI CCGCGG 1 cut(s) 3759
SinI GGWCC 5 cut(s) 2294, 2844, 3196, 3367, 3636
SmiMI CAYNNNNRTG 6 cut(s) 672, 731, 914, 1029, 2283, 2361
SmlI CTYRAG 3 cut(s) 1349, 1370, 2094
SmoI CTYRAG 3 cut(s) 1349, 1370, 2094
SpeI ACTAGT 1 cut(s) 1015
SphI GCATGC 1 cut(s) 1847
SspI AATATT 2 cut(s) 711, 1609
SspMI CTAG 4 cut(s) 548, 599, 750, 1016
SstI GAGCTC 2 cut(s) 3285, 3303
TaiI ACGT 3 cut(s) 2516, 3507, 3686
TatI WGTACW 4 cut(s) 126, 595, 1273, 1492
TauI GCSGC 9 cut(s) 693, 3247, 3250, 3253, 3256, 3572, 3590, 3626, 3758
TfiI GAWTC 8 cut(s) 830, 1039, 1799, 2149, 2428, 2690, 2852, 3104
TspDTI ATGAA 6 cut(s) 146, 1031, 1662, 1808, 2454, 2682
TspGWI ACGGA 2 cut(s) 666, 1168
Van91I CCANNNNNTGG 4 cut(s) 1236, 3188, 3352, 3646
Vha464I CTTAAG 2 cut(s) 1349, 1370
VneI GTGCAC 2 cut(s) 2286, 3604
VpaK11BI GGWCC 5 cut(s) 2294, 2844, 3196, 3367, 3636
VspI ATTAAT 1 cut(s) 1743
XapI RAATTY 4 cut(s) 305, 433, 3146, 3427
XbaI TCTAGA 1 cut(s) 547
XceI RCATGY 5 cut(s) 257, 491, 1847, 1955, 2729
XcmI CCANNNNNNNNNTGG 3 cut(s) 13, 2217, 3185
XmiI GTMKAC 2 cut(s) 1986, 2331
XmnI GAANNNNTTC 1 cut(s) 877
XspI CTAG 4 cut(s) 548, 599, 750, 1016
ZraI GACGTC 1 cut(s) 3684
ZrmI AGTACT 1 cut(s) 1494
Zsp2I ATGCAT 4 cut(s) 631, 1726, 1849, 2881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.