Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0014256

Physical Location & Seq

Genomic Coordinates Forward (+)

12021605 .. 12025902

Gene Structure 4298 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1128000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1749 bp

ATGCAAACCTTCTCGGCCTCCTTCGCCGTCTGTTCTTCCTACCCATTACGACGTCGTCGAATCAGCACCTCCCCTCCCCGCAAACCCAATCTCCGGCGACTCACCTCCCGCGTTGTCCAGCTCACCCGCCGGAGACAGCTTCACCAGATAATGGAGGAGATAGAGGTCGCTAAAGAACGATTCGGGAAGCTGAACACGGTTGTGATGAACGCGGTCGTGGAGGCTTGTGTACATTGCGGCGATATTGACTCTGCACTCATGTTGTTCGACGAAATGGCTGAGCCAGGAAGCTGTGGCGTTGATACTATCACATATGGAACCATCTTAAAGGGTTTGGGCGCGGCACGGAGAATTGATGAAGCATTTTATGTACTTGAATCTGTGGAGCGAGGCACTGCAGTGGGAAGTCCGAAATTGTCAGCACCTCTTGTTCTTGGTCTGCTCAATGCTCTAGTTGAAGCAGGAGATTTACGCCGCTCTAATGGGCTTCTTCATCACTATGGTTTTCTCCTTCGCGAAGGTGGCAGTCTTACAGTCAGAGTCTACAACTTACTAATGAAGGGATATATCAATGCAGGCCTTCCTCAGGGTGCAATAACCATGCATGACGAGATATTACGTCTAGGATTAAAGCCTGATAGGCTCACCTATAATACTCTGATATCTGCATGTGTAAAAGCTGGAAAGTTGGACATTGCAATGCAGTTTTTTGAGGAAATGAAGGATGAAGCACAGAAATTTAGCCATGGTGACCTTTTCCCAGATGTGGTCACTTACACTACATTACTTAAGGGATTTGGGGATTCCAAAGATATCAATTCAGTTCAGAAGATCATATTGGAAATGAAAACATATATTGGTTTGTTCATTGATCGAACAGCATACACTGCAGCAGCTGATGCTTTGCTAAATTGTGGCTCAATAAAAGGTGCTTTATGCATATTGGGAGAAATATTAAAGCGGGCTGGGGGAAAGCCAAATTTACGACCAAAACCTCACCTCTATCTTTCCTTCATGCGCGCATTTTCTGTTAGTGGAGATTACAGTACCGTTGAAAATCTAAATGAGCACATGTGGCGAGATACTTCTGGAACTATCTCCCCAATGGTTCAAGAGGAAGCTGATCATCTACTCATGGAGGCAGCTTTAAATGATGGTCAGGTAGATTTGGCTGTAAAATACCTCGCAAGTACTATTACAAGGTGGAAGAGGATATCATGGACAAGTCGAGGAGGCATGGTGGCTCTCCGCATAGAGGCCTTGCTGGGATTCACCAAATCGATATTGAGTCCTTATCTACTTCCTCAGGTATTGGCAAGTGAACCAATTGAAAGTGTCATGATGCCCTTTGAAGCAGCTCAGCCACTTAATGGGACCCTGGACTTGAACAAAGTGGTGATGCGTTTCTTTAAGGATTCAGTGGTGCCCATCATAGATGACTGGGGTAGCTGCATTGGTGTATTGCACCGTGAAGACTGTCGAGAGATGAACGCCCCTCTATCGTCAATGATGAGAAGCCCGCCTCCTTGTGTTGCTACTCGAACGTCAATCGGGCATGTGGTGGATCTGATCATGAAGTATAGATACAAAATGGTGATTGTAATAAACCATAGCTCAGTATATGGTGGTAGTGCTTCCAGTTTGAGAGCTGCTGGTGTTTTTACAGCCGAACAACTGTGTAAACTAGTGTCACCGGAATCCGAAACGCCAGGTCTGAGTCGACTTTCTTTACGTAGAAATCTCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS PPR PPR_2

Protein Analysis

583

Amino Acids

64.31

Weight (kDa)

8.81

Isoelectric Point (pI)

44.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	65 - 112	6.5e-08	PPR repeat family
PPR_2	PF13041	179 - 226	5.6e-10	PPR repeat family
PPR_3	PF13812	203 - 243	5.2e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	209 - 241	7e-11	PPR repeat
PPR_2	PF13041	212 - 244	4.6e-08	PPR repeat family
PPR	PF01535	216 - 242	4.5e-08	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014256)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10690 AT5G10690
fragaria_vesca	FvH4_2g36670 FvH4_2g36670
malus_domestica	MD08G1128000.v1.1
prunus_persica	Prupe.1G459800_v2.0.a1
pyrus_communis	pycom08g10700
rosa_chinensis	RchiOBHm_Chr6g0305021
rosa_laevigata	RLG00000010927
rosa_multiflora	Rmu_sc0000061.1_g000039
rosa_roxburghii	Rroxscaffold_7G00163250
rosa_rugosa	Rorug06G0336800
rosa_samantha	Rh6AG448400 Rh6BG452500 Rh6CG462100 Rh6DG449000
rosa_wichuraiana	Rw6G039050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	55
AccB1I	GGYRCC	1 cut(s)	1423
AccB7I	CCANNNNNTGG	2 cut(s)	151, 1370
AccBSI	CCGCTC	1 cut(s)	477
AccI	GTMKAC	2 cut(s)	543, 1720
AccII	CGCG	5 cut(s)	111, 212, 341, 516, 1020
AclWI	GGATC	1 cut(s)	1572
AcsI	RAATTY	2 cut(s)	737, 979
AcyI	GRCGYC	1 cut(s)	52
AfaI	GTAC	4 cut(s)	231, 372, 1048, 1192
AfiI	CCNNNNNNNGG	6 cut(s)	93, 151, 586, 766, 1255, 1370
AflII	CTTAAG	1 cut(s)	788
AflIII	ACRYGT	1 cut(s)	1071
AgsI	TTSAA	7 cut(s)	377, 458, 1055, 1112, 1331, 1352, 1387
AhlI	ACTAGT	1 cut(s)	1684
AjnI	CCWGG	3 cut(s)	283, 1377, 1708
AjuI	GAANNNNNNNTTGG	2 cut(s)	821, 853
AleI	CACNNNNGTG	2 cut(s)	200, 398
Alw21I	GWGCWC	1 cut(s)	1071
Alw26I	GTCTC	1 cut(s)	127
AlwI	GGATC	1 cut(s)	1572
AlwNI	CAGNNNCTG	1 cut(s)	896
AoxI	GGCC	3 cut(s)	15, 577, 1257
ApeKI	GCWGC	6 cut(s)	890, 893, 1142, 1355, 1449, 1649
ApoI	RAATTY	2 cut(s)	737, 979
AspLEI	GCGC	3 cut(s)	341, 1020, 1022
AspS9I	GGNCC	1 cut(s)	1374
AvaII	GGWCC	1 cut(s)	1374
AxyI	CCTNAGG	2 cut(s)	585, 1305
BaeGI	GKGCMC	1 cut(s)	1428
BanI	GGYRCC	1 cut(s)	1423
BarI	GAAGNNNNNNTAC	2 cut(s)	1568, 1600
BbsI	GAAGAC	1 cut(s)	1479
Bbv12I	GWGCWC	1 cut(s)	1071
BbvI	GCAGC	6 cut(s)	902, 905, 1154, 1367, 1436, 1636
BccI	CCATC	3 cut(s)	329, 1148, 1436
BceAI	ACGGC	1 cut(s)	11
BciT130I	CCWGG	3 cut(s)	285, 1379, 1710
BclI	TGATCA	2 cut(s)	1123, 1569
BcoDI	GTCTC	1 cut(s)	127
BcuI	ACTAGT	1 cut(s)	1684
BfaI	CTAG	3 cut(s)	452, 623, 1685
BfmI	CTRYAG	2 cut(s)	396, 888
BfrI	CTTAAG	1 cut(s)	788
BglI	GCCNNNNNGGC	1 cut(s)	640
BisI	GCNGC	9 cut(s)	238, 342, 475, 891, 894, 1143, 1356, 1450, 1650
BlpI	GCTNAGC	2 cut(s)	279, 1359
BlsI	GCNGC	9 cut(s)	239, 343, 476, 892, 895, 1144, 1357, 1451, 1651
BmcAI	AGTACT	1 cut(s)	1192
Bme1390I	CCNGG	3 cut(s)	285, 1379, 1710
Bme18I	GGWCC	1 cut(s)	1374
BmgT120I	GGNCC	1 cut(s)	1374
BmiI	GGNNCC	4 cut(s)	319, 1375, 1376, 1425
BmrFI	CCNGG	3 cut(s)	285, 1379, 1710
BmrI	ACTGGG	1 cut(s)	1450
BmsI	GCATC	3 cut(s)	889, 1332, 1389
BmuI	ACTGGG	1 cut(s)	1450
BpiI	GAAGAC	1 cut(s)	1479
Bpu1102I	GCTNAGC	2 cut(s)	279, 1359
Bsa29I	ATCGAT	1 cut(s)	1280
BsaAI	YACGTR	1 cut(s)	1733
BsaHI	GRCGYC	1 cut(s)	52
BsaJI	CCNNGG	2 cut(s)	745, 1377
BsaWI	WCCGGW	1 cut(s)	1693
BsaXI	ACNNNNNCTCC	6 cut(s)	58, 75, 88, 105, 1224, 1254
Bsc4I	CCNNNNNNNGG	6 cut(s)	93, 151, 586, 766, 1255, 1370
Bse1I	ACTGG	2 cut(s)	1445, 1638
Bse21I	CCTNAGG	2 cut(s)	585, 1305
Bse3DI	GCAATG	3 cut(s)	232, 693, 705
BseBI	CCWGG	3 cut(s)	285, 1379, 1710
BseCI	ATCGAT	1 cut(s)	1280
BseDI	CCNNGG	2 cut(s)	745, 1377
BseGI	GGATG	1 cut(s)	730
BseLI	CCNNNNNNNGG	6 cut(s)	93, 151, 586, 766, 1255, 1370
BseMI	GCAATG	3 cut(s)	232, 693, 705
BseMII	CTCAG	6 cut(s)	270, 599, 1319, 1373, 1629, 1706
BseNI	ACTGG	2 cut(s)	1445, 1638
BsePI	GCGCGC	1 cut(s)	1018
BseRI	GAGGAG	2 cut(s)	170, 1245
BseSI	GKGCMC	1 cut(s)	1428
BseXI	GCAGC	6 cut(s)	902, 905, 1154, 1367, 1436, 1636
BseYI	CCCAGC	2 cut(s)	965, 1264
BsgI	GTGCAG	1 cut(s)	237
Bsh1236I	CGCG	5 cut(s)	111, 212, 341, 516, 1020
Bsh1285I	CGRYCG	1 cut(s)	216
BshFI	GGCC	3 cut(s)	17, 579, 1259
BshNI	GGYRCC	1 cut(s)	1423
BshVI	ATCGAT	1 cut(s)	1280
BsiEI	CGRYCG	1 cut(s)	216
BsiHKAI	GWGCWC	1 cut(s)	1071
BsiSI	CCGG	3 cut(s)	94, 130, 1694
BslFI	GGGAC	1 cut(s)	1387
BslI	CCNNNNNNNGG	6 cut(s)	93, 151, 586, 766, 1255, 1370
BsmAI	GTCTC	1 cut(s)	127
BsmFI	GGGAC	1 cut(s)	1387
BsnI	GGCC	3 cut(s)	17, 579, 1259
Bsp1286I	GDGCHC	2 cut(s)	1071, 1428
Bsp1407I	TGTACA	1 cut(s)	229
Bsp143I	GATC	5 cut(s)	831, 871, 1123, 1564, 1569
Bsp1720I	GCTNAGC	2 cut(s)	279, 1359
Bsp19I	CCATGG	1 cut(s)	745
Bsp68I	TCGCGA	1 cut(s)	516
BspANI	GGCC	3 cut(s)	17, 579, 1259
BspCNI	CTCAG	6 cut(s)	271, 598, 1318, 1372, 1628, 1707
BspDI	ATCGAT	1 cut(s)	1280
BspFNI	CGCG	5 cut(s)	111, 212, 341, 516, 1020
BspHI	TCATGA	2 cut(s)	1338, 1572
BspLI	GGNNCC	4 cut(s)	319, 1375, 1376, 1425
BspMAI	CTGCAG	2 cut(s)	400, 892
BspPI	GGATC	1 cut(s)	1572
BspT107I	GGYRCC	1 cut(s)	1423
BspTI	CTTAAG	1 cut(s)	788
BsrBI	CCGCTC	1 cut(s)	477
BsrDI	GCAATG	3 cut(s)	232, 693, 705
BsrGI	TGTACA	1 cut(s)	229
BsrI	ACTGG	2 cut(s)	1445, 1638
BssECI	CCNNGG	2 cut(s)	745, 1377
BssHII	GCGCGC	1 cut(s)	1018
BssMI	GATC	5 cut(s)	831, 871, 1123, 1564, 1569
BssNI	GRCGYC	1 cut(s)	52
BssT1I	CCWWGG	1 cut(s)	745
Bst2UI	CCWGG	3 cut(s)	285, 1379, 1710
Bst4CI	ACNGT	7 cut(s)	199, 535, 1046, 1051, 1469, 1478, 1677
Bst6I	CTCTTC	1 cut(s)	1202
BstACI	GRCGYC	1 cut(s)	52
BstAFI	CTTAAG	1 cut(s)	788
BstAPI	GCANNNNNTGC	2 cut(s)	887, 899
BstAUI	TGTACA	1 cut(s)	229
BstBAI	YACGTR	1 cut(s)	1733
BstC8I	GCNNGC	4 cut(s)	577, 963, 1020, 1520
BstDEI	CTNAG	6 cut(s)	279, 585, 1305, 1359, 1615, 1715
BstDSI	CCRYGG	1 cut(s)	745
BstEII	GGTNACC	1 cut(s)	749
BstENI	CCTNNNNNAGG	1 cut(s)	584
BstF5I	GGATG	1 cut(s)	730
BstFNI	CGCG	5 cut(s)	111, 212, 341, 516, 1020
BstHHI	GCGC	3 cut(s)	341, 1020, 1022
BstKTI	GATC	5 cut(s)	834, 874, 1126, 1567, 1572
BstMAI	GTCTC	1 cut(s)	127
BstMBI	GATC	5 cut(s)	831, 871, 1123, 1564, 1569
BstMCI	CGRYCG	1 cut(s)	216
BstMWI	GCNNNNNNNGC	6 cut(s)	23, 522, 640, 887, 899, 1075
BstNI	CCWGG	3 cut(s)	285, 1379, 1710
BstNSI	RCATGY	3 cut(s)	672, 1075, 1559
BstPI	GGTNACC	1 cut(s)	749
BstSCI	CCNGG	3 cut(s)	283, 1377, 1708
BstSFI	CTRYAG	2 cut(s)	396, 888
BstSLI	GKGCMC	1 cut(s)	1428
BstSNI	TACGTA	1 cut(s)	1733
BstUI	CGCG	5 cut(s)	111, 212, 341, 516, 1020
BstV1I	GCAGC	6 cut(s)	902, 905, 1154, 1367, 1436, 1636
BstV2I	GAAGAC	1 cut(s)	1479
BstX2I	RGATCY	1 cut(s)	1564
BstYI	RGATCY	1 cut(s)	1564
Bsu15I	ATCGAT	1 cut(s)	1280
Bsu36I	CCTNAGG	2 cut(s)	585, 1305
BsuRI	GGCC	3 cut(s)	17, 579, 1259
BsuTUI	ATCGAT	1 cut(s)	1280
BtgI	CCRYGG	1 cut(s)	745
BtsCI	GGATG	1 cut(s)	730
BtsI	GCAGTG	3 cut(s)	393, 405, 885
BtsIMutI	CAGTG	4 cut(s)	393, 405, 885, 1425
BtuMI	TCGCGA	1 cut(s)	516
Cac8I	GCNNGC	4 cut(s)	577, 963, 1020, 1520
CaiI	CAGNNNCTG	1 cut(s)	896
CciI	TCATGA	2 cut(s)	1338, 1572
CfoI	GCGC	3 cut(s)	341, 1020, 1022
Cfr13I	GGNCC	1 cut(s)	1374
ClaI	ATCGAT	1 cut(s)	1280
Csp6I	GTAC	4 cut(s)	230, 371, 1047, 1191
CviQI	GTAC	4 cut(s)	230, 371, 1047, 1191
DdeI	CTNAG	6 cut(s)	279, 585, 1305, 1359, 1615, 1715
DpnI	GATC	5 cut(s)	833, 873, 1125, 1566, 1571
DpnII	GATC	5 cut(s)	831, 871, 1123, 1564, 1569
DraI	TTTAAA	1 cut(s)	1149
Eam1104I	CTCTTC	1 cut(s)	1202
EarI	CTCTTC	1 cut(s)	1202
Eco105I	TACGTA	1 cut(s)	1733
Eco130I	CCWWGG	1 cut(s)	745
Eco147I	AGGCCT	2 cut(s)	579, 1259
Eco32I	GATATC	3 cut(s)	663, 814, 1215
Eco47I	GGWCC	1 cut(s)	1374
Eco81I	CCTNAGG	2 cut(s)	585, 1305
Eco91I	GGTNACC	1 cut(s)	749
EcoNI	CCTNNNNNAGG	1 cut(s)	584
EcoO109I	RGGNCCY	1 cut(s)	1374
EcoO65I	GGTNACC	1 cut(s)	749
EcoRII	CCWGG	3 cut(s)	283, 1377, 1708
EcoRV	GATATC	3 cut(s)	663, 814, 1215
EcoT14I	CCWWGG	1 cut(s)	745
EcoT22I	ATGCAT	2 cut(s)	606, 941
ErhI	CCWWGG	1 cut(s)	745
FaqI	GGGAC	1 cut(s)	1387
FauI	CCCGC	5 cut(s)	86, 116, 134, 954, 1527
FauNDI	CATATG	1 cut(s)	313
FbaI	TGATCA	2 cut(s)	1123, 1569
FblI	GTMKAC	2 cut(s)	543, 1720
Fnu4HI	GCNGC	9 cut(s)	238, 342, 475, 891, 894, 1143, 1356, 1450, 1650
FokI	GGATG	1 cut(s)	737
Fsp4HI	GCNGC	9 cut(s)	238, 342, 475, 891, 894, 1143, 1356, 1450, 1650
FspBI	CTAG	3 cut(s)	452, 623, 1685
GlaI	GCGC	3 cut(s)	340, 1019, 1021
GluI	GCNGC	9 cut(s)	238, 342, 475, 891, 894, 1143, 1356, 1450, 1650
GsaI	CCCAGC	2 cut(s)	969, 1268
HaeIII	GGCC	3 cut(s)	17, 579, 1259
HapII	CCGG	3 cut(s)	94, 130, 1694
HhaI	GCGC	3 cut(s)	341, 1020, 1022
Hin1I	GRCGYC	1 cut(s)	52
Hin6I	GCGC	3 cut(s)	339, 1018, 1020
HinP1I	GCGC	3 cut(s)	339, 1018, 1020
HincII	GTYRAC	1 cut(s)	1721
HindII	GTYRAC	1 cut(s)	1721
HpaII	CCGG	3 cut(s)	94, 130, 1694
Hpy166II	GTNNAC	5 cut(s)	230, 544, 1322, 1682, 1721
Hpy188I	TCNGA	7 cut(s)	411, 539, 660, 828, 1569, 1702, 1716
Hpy188III	TCNNGA	7 cut(s)	184, 515, 1089, 1112, 1339, 1481, 1573
Hpy8I	GTNNAC	5 cut(s)	230, 544, 1322, 1682, 1721
Hpy99I	CGWCG	4 cut(s)	54, 57, 60, 272
HpyAV	CCTTC	8 cut(s)	19, 31, 512, 521, 553, 590, 715, 1021
HpyCH4III	ACNGT	7 cut(s)	199, 535, 1046, 1051, 1469, 1478, 1677
HpyCH4IV	ACGT	4 cut(s)	52, 619, 1544, 1732
HpyF10VI	GCNNNNNNNGC	6 cut(s)	23, 522, 640, 887, 899, 1075
HpyF3I	CTNAG	6 cut(s)	279, 585, 1305, 1359, 1615, 1715
HpySE526I	ACGT	4 cut(s)	52, 619, 1544, 1732
Hsp92I	GRCGYC	1 cut(s)	52
HspAI	GCGC	3 cut(s)	339, 1018, 1020
KflI	GGGWCCC	1 cut(s)	1374
Ksp22I	TGATCA	2 cut(s)	1123, 1569
Kzo9I	GATC	5 cut(s)	831, 871, 1123, 1564, 1569
LmnI	GCTCC	1 cut(s)	385
Lsp1109I	GCAGC	6 cut(s)	902, 905, 1154, 1367, 1436, 1636
LweI	GCATC	3 cut(s)	889, 1332, 1389
MaeI	CTAG	3 cut(s)	452, 623, 1685
MaeII	ACGT	4 cut(s)	52, 619, 1544, 1732
MaeIII	GTNAC	3 cut(s)	749, 769, 1689
MalI	GATC	5 cut(s)	833, 873, 1125, 1566, 1571
MbiI	CCGCTC	1 cut(s)	477
MboI	GATC	5 cut(s)	831, 871, 1123, 1564, 1569
MboII	GAAGA	5 cut(s)	27, 482, 841, 1219, 1484
MfeI	CAATTG	1 cut(s)	1326
MflI	RGATCY	1 cut(s)	1564
MhlI	GDGCHC	2 cut(s)	1071, 1428
MluCI	AATT	7 cut(s)	351, 413, 737, 817, 910, 979, 1326
MlyI	GAGTC	5 cut(s)	93, 242, 549, 1297, 1726
MmeI	TCCRAC	1 cut(s)	669
Mph1103I	ATGCAT	2 cut(s)	606, 941
MseI	TTAA	7 cut(s)	326, 629, 789, 956, 1148, 1368, 1410
MslI	CAYNNNNRTG	4 cut(s)	200, 398, 498, 698
MspA1I	CMGCKG	1 cut(s)	896
MspCI	CTTAAG	1 cut(s)	788
MspI	CCGG	3 cut(s)	94, 130, 1694
MspR9I	CCNGG	3 cut(s)	285, 1379, 1710
MunI	CAATTG	1 cut(s)	1326
MvaI	CCWGG	3 cut(s)	285, 1379, 1710
MvnI	CGCG	5 cut(s)	111, 212, 341, 516, 1020
MwoI	GCNNNNNNNGC	6 cut(s)	23, 522, 640, 887, 899, 1075
NcoI	CCATGG	1 cut(s)	745
NdeI	CATATG	1 cut(s)	313
NdeII	GATC	5 cut(s)	831, 871, 1123, 1564, 1569
NlaIV	GGNNCC	4 cut(s)	319, 1375, 1376, 1425
NmuCI	GTSAC	3 cut(s)	749, 769, 1689
NruI	TCGCGA	1 cut(s)	516
NsiI	ATGCAT	2 cut(s)	606, 941
NspI	RCATGY	3 cut(s)	672, 1075, 1559
OliI	CACNNNNGTG	2 cut(s)	200, 398
PagI	TCATGA	2 cut(s)	1338, 1572
PauI	GCGCGC	1 cut(s)	1018
PceI	AGGCCT	2 cut(s)	579, 1259
PciI	ACATGT	1 cut(s)	1071
PcsI	WCGNNNNNNNCGW	1 cut(s)	55
PfeI	GAWTC	7 cut(s)	60, 180, 377, 803, 1269, 1415, 1697
PflFI	GACNNNGTC	1 cut(s)	54
PflMI	CCANNNNNTGG	2 cut(s)	151, 1370
PkrI	GCNGC	9 cut(s)	239, 343, 476, 892, 895, 1144, 1357, 1451, 1651
PleI	GAGTC	5 cut(s)	93, 242, 548, 1296, 1725
PpsI	GAGTC	5 cut(s)	93, 242, 548, 1296, 1725
Ppu21I	YACGTR	1 cut(s)	1733
PpuMI	RGGWCCY	1 cut(s)	1374
PscI	ACATGT	1 cut(s)	1071
Psp5II	RGGWCCY	1 cut(s)	1374
Psp6I	CCWGG	3 cut(s)	283, 1377, 1708
PspEI	GGTNACC	1 cut(s)	749
PspFI	CCCAGC	2 cut(s)	965, 1264
PspGI	CCWGG	3 cut(s)	283, 1377, 1708
PspN4I	GGNNCC	4 cut(s)	319, 1375, 1376, 1425
PspPI	GGNCC	1 cut(s)	1374
PspPPI	RGGWCCY	1 cut(s)	1374
PstI	CTGCAG	2 cut(s)	400, 892
PstNI	CAGNNNCTG	1 cut(s)	896
PsuI	RGATCY	1 cut(s)	1564
PsyI	GACNNNGTC	1 cut(s)	54
PteI	GCGCGC	1 cut(s)	1018
PvuII	CAGCTG	1 cut(s)	896
RruI	TCGCGA	1 cut(s)	516
RsaI	GTAC	4 cut(s)	231, 372, 1048, 1192
RsaNI	GTAC	4 cut(s)	230, 371, 1047, 1191
RseI	CAYNNNNRTG	4 cut(s)	200, 398, 498, 698
SalI	GTCGAC	1 cut(s)	1719
SaqAI	TTAA	7 cut(s)	326, 629, 789, 956, 1148, 1368, 1410
SatI	GCNGC	9 cut(s)	238, 342, 475, 891, 894, 1143, 1356, 1450, 1650
Sau3AI	GATC	5 cut(s)	831, 871, 1123, 1564, 1569
Sau96I	GGNCC	1 cut(s)	1374
ScaI	AGTACT	1 cut(s)	1192
SchI	GAGTC	5 cut(s)	93, 242, 549, 1297, 1726
ScrFI	CCNGG	3 cut(s)	285, 1379, 1710
SduI	GDGCHC	2 cut(s)	1071, 1428
SfaNI	GCATC	3 cut(s)	889, 1332, 1389
SfcI	CTRYAG	2 cut(s)	396, 888
SinI	GGWCC	1 cut(s)	1374
SmiMI	CAYNNNNRTG	4 cut(s)	200, 398, 498, 698
SmlI	CTYRAG	1 cut(s)	788
SmoI	CTYRAG	1 cut(s)	788
SnaBI	TACGTA	1 cut(s)	1733
SpeI	ACTAGT	1 cut(s)	1684
Sse9I	AATT	7 cut(s)	351, 413, 737, 817, 910, 979, 1326
SseBI	AGGCCT	2 cut(s)	579, 1259
SspI	AATATT	1 cut(s)	954
SspMI	CTAG	3 cut(s)	452, 623, 1685
StuI	AGGCCT	2 cut(s)	579, 1259
StyD4I	CCNGG	3 cut(s)	283, 1377, 1708
StyI	CCWWGG	1 cut(s)	745
TaaI	ACNGT	7 cut(s)	199, 535, 1046, 1051, 1469, 1478, 1677
TaiI	ACGT	4 cut(s)	55, 622, 1547, 1735
TaqI	TCGA	8 cut(s)	58, 267, 874, 1228, 1280, 1480, 1540, 1720
TasI	AATT	7 cut(s)	351, 413, 737, 817, 910, 979, 1326
TatI	WGTACW	3 cut(s)	229, 370, 1190
TauI	GCSGC	3 cut(s)	240, 344, 477
TfiI	GAWTC	7 cut(s)	60, 180, 377, 803, 1269, 1415, 1697
Tru1I	TTAA	7 cut(s)	326, 629, 789, 956, 1148, 1368, 1410
Tru9I	TTAA	7 cut(s)	326, 629, 789, 956, 1148, 1368, 1410
TscAI	CASTG	4 cut(s)	400, 405, 892, 1425
TseFI	GTSAC	3 cut(s)	749, 769, 1689
TseI	GCWGC	6 cut(s)	890, 893, 1142, 1355, 1449, 1649
Tsp45I	GTSAC	3 cut(s)	749, 769, 1689
TspGWI	ACGGA	1 cut(s)	361
TspRI	CASTG	4 cut(s)	400, 405, 892, 1425
Tth111I	GACNNNGTC	1 cut(s)	54
Van91I	CCANNNNNTGG	2 cut(s)	151, 1370
Vha464I	CTTAAG	1 cut(s)	788
VpaK11BI	GGWCC	1 cut(s)	1374
XagI	CCTNNNNNAGG	1 cut(s)	584
XapI	RAATTY	2 cut(s)	737, 979
XceI	RCATGY	3 cut(s)	672, 1075, 1559
XmiI	GTMKAC	2 cut(s)	543, 1720
XspI	CTAG	3 cut(s)	452, 623, 1685
ZraI	GACGTC	1 cut(s)	53
ZrmI	AGTACT	1 cut(s)	1192
Zsp2I	ATGCAT	2 cut(s)	606, 941

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.