Rh6AG448400

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0014256
Physical Location & Seq
Reverse (-)
63470396 .. 63474754
4359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG448400.1

Sequence Viewer

Length: 1281 bp
ATGCAAGCCTTCTCGCCCTCCTTCGCCGTCTGTTCCTATTTTCCGATACGACGCTGTCGCATCACCACCGCCTCTCCCCGCAAATCCAACCTCAAGCGACTCACCTCCCGCGTCGTCCAACTCACTCGCCGACGACAGCTCCACCAGATAATGGAGGAGATAGAGATCGCTAGAGAACGGTACGGGAAGCTGAACACGATAGTGATGAACGCGGTGGTGGAGGCGTGTGTCCATTGCGGCGACATCGACTCTGCACTTAAGGTGTTCGACGAAATGTCTAAGCCGGGGAGCTGTGGCGTTGATACTGTCACATATGCTACACTTTTGAAGGTACTATTTCTCTTTTTTCTTTTTTTGAATTTGGATGCCATGGATTTGATAATGTGTTTTGTTATAAAGGGATTGGGCGGGGCGAGGAGAATCGATGAGGCATTTGAAGTGCTTGAATCCGTTGAACGCGGTGTTGCTGTGGGAAGTCCAAAGTTGTCAGCACCTCTTATTTTTGGACTGCTGAATGCTTTGGTTGAAGCAGGAGATTTACGCCGAGCTAATGGACTTCTTGCACGGTATGGTTTTCTGCTTCGAGAAGGTGGCAGTCTTTCGCTCAGAGTCTACAACTTATTAATGAAGGGATGTATTAATTCAGGCCTTCCTCAGGGTGCGATAGACATGCACAACGAATTAATACGTCTAGGGTTGAAGCCTGATAGGCTCACCTATAATACTCTAATATCTGCATGTGTAAAAGCTGGGAAGTTGGATGCCGCAATGCAATACTTTGAGGAAATGAAGGATGAAGCGCAGAAATTTAGTGATGCTGGTCTATTCCCTGATGTCATCACCTATAGTACATTGCTTAAGGGTTTTGGGGATTCCAAAGACATCCTTATGGTTCATAAGATTGTTTCAGAAATGAAAACATATATTGGTTTGTTCATTGATCGAACAGCATATACGGCAGCAGTTGATGCTTTGCTAAATTGTGACGCAATAAAGGGTGCTTTATGTATTTTTGGAGAATTACTAAAGCAGGCTGGTGGAAATCCAGAGTTGCGACCCAAACCTCACCTCTATCTTTCCTTGATGCGTGCCTTTGCTGTTAGAGGAGATTATGACACAGTAAACAGTCTGCATAAGCACATGTGGCTAGATACTGCTGGAACTATCTACCCAATGATTCATGAAGAAGCTGATCATCTTGTAATGGAGTCAGCTTTAAATGATGGGCAGGTACGATATGAACTCCCCTATGGTCTTCTTCAATGGGGTGTTACTGCATAA

Protein Analysis

426

Amino Acids

47.34

Weight (kDa)

7.55

Isoelectric Point (pI)

31.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 65 - 112 4.4e-08 PPR repeat family
PPR_2 PF13041 204 - 249 3.1e-10 PPR repeat family
PPR_3 PF13812 227 - 266 1.1e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 232 - 264 6.4e-10 PPR repeat
PPR_2 PF13041 239 - 267 2.3e-07 PPR repeat family
PPR PF01535 239 - 265 1.1e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10690 AT5G10690
fragaria_vesca FvH4_2g36670 FvH4_2g36670
malus_domestica MD08G1128000.v1.1
prunus_persica Prupe.1G459800_v2.0.a1
pyrus_communis pycom08g10700
rosa_chinensis RchiOBHm_Chr6g0305021
rosa_laevigata RLG00000010927
rosa_multiflora Rmu_sc0000061.1_g000039
rosa_roxburghii Rroxscaffold_7G00163250
rosa_rugosa Rorug06G0336800
rosa_samantha Rh6AG448400 Rh6BG452500 Rh6CG462100 Rh6DG449000
rosa_wichuraiana Rw6G039050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 395
Acc36I ACCTGC 1 cut(s) 1219
AccB7I CCANNNNNTGG 1 cut(s) 151
AccI GTMKAC 1 cut(s) 612
AccII CGCG 3 cut(s) 111, 212, 459
AciI CCGC 8 cut(s) 69, 79, 109, 212, 237, 408, 459, 765
AcsI RAATTY 2 cut(s) 358, 806
AfaI GTAC 4 cut(s) 182, 333, 850, 1233
AfiI CCNNNNNNNGG 2 cut(s) 151, 655
AflII CTTAAG 2 cut(s) 257, 857
AflIII ACRYGT 1 cut(s) 1140
AgsI TTSAA 8 cut(s) 328, 358, 437, 446, 455, 527, 700, 1262
AhdI GACNNNNNGTC 1 cut(s) 274
AleI CACNNNNGTG 1 cut(s) 200
AluBI AGCT 7 cut(s) 139, 190, 291, 548, 749, 1190, 1214
AluI AGCT 7 cut(s) 139, 190, 291, 548, 749, 1190, 1214
AoxI GGCC 1 cut(s) 646
ApeKI GCWGC 1 cut(s) 959
ApoI RAATTY 2 cut(s) 358, 806
AseI ATTAAT 3 cut(s) 623, 639, 683
AspLEI GCGC 1 cut(s) 802
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 5 cut(s) 55, 94, 706, 832, 1058
AxyI CCTNAGG 1 cut(s) 654
BbsI GAAGAC 1 cut(s) 1247
BbvI GCAGC 1 cut(s) 971
BccI CCATC 1 cut(s) 1217
BceAI ACGGC 2 cut(s) 11, 972
BcgI CGANNNNNNTGC 2 cut(s) 652, 686
BclI TGATCA 1 cut(s) 1192
BcnI CCSGG 1 cut(s) 285
BfaI CTAG 3 cut(s) 171, 692, 1148
BfmI CTRYAG 1 cut(s) 844
BfrI CTTAAG 2 cut(s) 257, 857
BfuAI ACCTGC 1 cut(s) 1219
BglI GCCNNNNNGGC 1 cut(s) 709
BisI GCNGC 3 cut(s) 238, 765, 960
BlsI GCNGC 3 cut(s) 239, 766, 961
Bme1390I CCNGG 1 cut(s) 285
BmeRI GACNNNNNGTC 1 cut(s) 274
BmrFI CCNGG 1 cut(s) 285
BmsI GCATC 6 cut(s) 69, 355, 751, 805, 958, 1074
BpiI GAAGAC 1 cut(s) 1247
BpuEI CTTGAG 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 285
Bsa29I ATCGAT 1 cut(s) 423
BsaBI GATNNNNATC 1 cut(s) 164
BsaJI CCNNGG 2 cut(s) 284, 369
BsaXI ACNNNNNCTCC 4 cut(s) 58, 88, 123, 153
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 655
Bse21I CCTNAGG 1 cut(s) 654
Bse3DI GCAATG 3 cut(s) 232, 774, 851
Bse8I GATNNNNATC 1 cut(s) 164
BseCI ATCGAT 1 cut(s) 423
BseDI CCNNGG 2 cut(s) 284, 369
BseGI GGATG 5 cut(s) 370, 638, 766, 799, 882
BseJI GATNNNNATC 1 cut(s) 164
BseLI CCNNNNNNNGG 2 cut(s) 151, 655
BseMI GCAATG 3 cut(s) 232, 774, 851
BseMII CTCAG 2 cut(s) 619, 668
BseRI GAGGAG 3 cut(s) 170, 430, 1119
BseXI GCAGC 1 cut(s) 971
BseYI CCCAGC 1 cut(s) 749
BsgI GTGCAG 1 cut(s) 237
Bsh1236I CGCG 3 cut(s) 111, 212, 459
BshFI GGCC 1 cut(s) 648
BshVI ATCGAT 1 cut(s) 423
BsiSI CCGG 1 cut(s) 284
BslI CCNNNNNNNGG 2 cut(s) 151, 655
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 1 cut(s) 648
Bsp143I GATC 3 cut(s) 165, 940, 1192
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 8 cut(s) 69, 79, 109, 212, 237, 408, 459, 765
BspANI GGCC 1 cut(s) 648
BspCNI CTCAG 2 cut(s) 618, 667
BspDI ATCGAT 1 cut(s) 423
BspFNI CGCG 3 cut(s) 111, 212, 459
BspHI TCATGA 1 cut(s) 1180
BspMI ACCTGC 1 cut(s) 1219
BspTI CTTAAG 2 cut(s) 257, 857
BsrDI GCAATG 3 cut(s) 232, 774, 851
BssECI CCNNGG 2 cut(s) 284, 369
BssMI GATC 3 cut(s) 165, 940, 1192
BssT1I CCWWGG 1 cut(s) 369
Bst4CI ACNGT 5 cut(s) 180, 307, 567, 1120, 1127
BstAFI CTTAAG 2 cut(s) 257, 857
BstAPI GCANNNNNTGC 1 cut(s) 968
BstC8I GCNNGC 3 cut(s) 6, 1032, 1089
BstDEI CTNAG 3 cut(s) 279, 605, 654
BstDSI CCRYGG 1 cut(s) 369
BstENI CCTNNNNNAGG 1 cut(s) 653
BstF5I GGATG 5 cut(s) 370, 638, 766, 799, 882
BstFNI CGCG 3 cut(s) 111, 212, 459
BstHHI GCGC 1 cut(s) 802
BstKTI GATC 3 cut(s) 168, 943, 1195
BstMBI GATC 3 cut(s) 165, 940, 1192
BstMWI GCNNNNNNNGC 4 cut(s) 709, 956, 968, 1144
BstNSI RCATGY 3 cut(s) 673, 741, 1144
BstSCI CCNGG 1 cut(s) 283
BstSFI CTRYAG 1 cut(s) 844
BstUI CGCG 3 cut(s) 111, 212, 459
BstV1I GCAGC 1 cut(s) 971
BstV2I GAAGAC 1 cut(s) 1247
Bsu15I ATCGAT 1 cut(s) 423
Bsu36I CCTNAGG 1 cut(s) 654
BsuRI GGCC 1 cut(s) 648
BsuTUI ATCGAT 1 cut(s) 423
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 5 cut(s) 370, 638, 766, 799, 882
BveI ACCTGC 1 cut(s) 1219
Cac8I GCNNGC 3 cut(s) 6, 1032, 1089
CciI TCATGA 1 cut(s) 1180
CfoI GCGC 1 cut(s) 802
ClaI ATCGAT 1 cut(s) 423
CseI GACGC 3 cut(s) 60, 100, 995
Csp6I GTAC 4 cut(s) 181, 332, 849, 1232
CviAII CATG 5 cut(s) 370, 670, 738, 1141, 1181
CviQI GTAC 4 cut(s) 181, 332, 849, 1232
DdeI CTNAG 3 cut(s) 279, 605, 654
DpnI GATC 3 cut(s) 167, 942, 1194
DpnII GATC 3 cut(s) 165, 940, 1192
DraI TTTAAA 1 cut(s) 1218
DriI GACNNNNNGTC 1 cut(s) 274
Eam1105I GACNNNNNGTC 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 369
Eco147I AGGCCT 1 cut(s) 648
Eco81I CCTNAGG 1 cut(s) 654
EcoNI CCTNNNNNAGG 1 cut(s) 653
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 5 cut(s) 373, 673, 741, 1144, 1184
FalI AAGNNNNNCTT 2 cut(s) 870, 902
FatI CATG 5 cut(s) 369, 669, 737, 1140, 1180
FauI CCCGC 3 cut(s) 86, 116, 401
FauNDI CATATG 1 cut(s) 313
FbaI TGATCA 1 cut(s) 1192
FblI GTMKAC 1 cut(s) 612
Fnu4HI GCNGC 3 cut(s) 238, 765, 960
FokI GGATG 5 cut(s) 377, 645, 773, 806, 869
Fsp4HI GCNGC 3 cut(s) 238, 765, 960
FspBI CTAG 3 cut(s) 171, 692, 1148
GlaI GCGC 1 cut(s) 801
GluI GCNGC 3 cut(s) 238, 765, 960
GsaI CCCAGC 1 cut(s) 753
HaeIII GGCC 1 cut(s) 648
HapII CCGG 1 cut(s) 284
HgaI GACGC 3 cut(s) 60, 100, 995
HhaI GCGC 1 cut(s) 802
Hin1II CATG 5 cut(s) 373, 673, 741, 1144, 1184
Hin6I GCGC 1 cut(s) 800
HinP1I GCGC 1 cut(s) 800
HinfI GANTC 8 cut(s) 99, 248, 420, 446, 609, 872, 1177, 1208
HpaII CCGG 1 cut(s) 284
HphI GGTGA 5 cut(s) 55, 94, 706, 832, 1058
Hpy166II GTNNAC 2 cut(s) 613, 1123
Hpy188I TCNGA 3 cut(s) 45, 608, 910
Hpy188III TCNNGA 3 cut(s) 584, 1046, 1181
Hpy8I GTNNAC 2 cut(s) 613, 1123
Hpy99I CGWCG 4 cut(s) 54, 116, 135, 272
HpyAV CCTTC 7 cut(s) 19, 31, 322, 581, 622, 659, 784
HpyCH4III ACNGT 5 cut(s) 180, 307, 567, 1120, 1127
HpyCH4IV ACGT 1 cut(s) 688
HpyCH4V TGCA 8 cut(s) 4, 254, 563, 673, 737, 772, 1132, 1277
HpyF10VI GCNNNNNNNGC 4 cut(s) 709, 956, 968, 1144
HpyF3I CTNAG 3 cut(s) 279, 605, 654
HpySE526I ACGT 1 cut(s) 688
Hsp92II CATG 5 cut(s) 373, 673, 741, 1144, 1184
HspAI GCGC 1 cut(s) 800
Ksp22I TGATCA 1 cut(s) 1192
Kzo9I GATC 3 cut(s) 165, 940, 1192
LmnI GCTCC 2 cut(s) 144, 288
Lsp1109I GCAGC 1 cut(s) 971
LweI GCATC 6 cut(s) 69, 355, 751, 805, 958, 1074
MaeI CTAG 3 cut(s) 171, 692, 1148
MaeII ACGT 1 cut(s) 688
MaeIII GTNAC 3 cut(s) 307, 983, 1270
MalI GATC 3 cut(s) 167, 942, 1194
MboI GATC 3 cut(s) 165, 940, 1192
MboII GAAGA 3 cut(s) 1196, 1247, 1250
MluCI AATT 6 cut(s) 358, 640, 680, 806, 979, 1019
MlyI GAGTC 4 cut(s) 93, 242, 618, 1217
MmeI TCCRAC 3 cut(s) 111, 142, 738
MseI TTAA 6 cut(s) 258, 623, 639, 683, 858, 1217
MslI CAYNNNNRTG 2 cut(s) 200, 887
MspCI CTTAAG 2 cut(s) 257, 857
MspI CCGG 1 cut(s) 284
MspR9I CCNGG 1 cut(s) 285
Mva1269I GAATGC 1 cut(s) 520
MvnI CGCG 3 cut(s) 111, 212, 459
MwoI GCNNNNNNNGC 4 cut(s) 709, 956, 968, 1144
NciI CCSGG 1 cut(s) 285
NcoI CCATGG 1 cut(s) 369
NdeI CATATG 1 cut(s) 313
NdeII GATC 3 cut(s) 165, 940, 1192
NlaIII CATG 5 cut(s) 373, 673, 741, 1144, 1184
NmeAIII GCCGAG 1 cut(s) 569
NmuCI GTSAC 2 cut(s) 307, 983
NspI RCATGY 3 cut(s) 673, 741, 1144
OliI CACNNNNGTG 1 cut(s) 200
PagI TCATGA 1 cut(s) 1180
PceI AGGCCT 1 cut(s) 648
PciI ACATGT 1 cut(s) 1140
PctI GAATGC 1 cut(s) 520
PfeI GAWTC 4 cut(s) 420, 446, 872, 1177
PflFI GACNNNGTC 1 cut(s) 54
PflMI CCANNNNNTGG 1 cut(s) 151
PkrI GCNGC 3 cut(s) 239, 766, 961
PleI GAGTC 4 cut(s) 93, 242, 617, 1216
PpsI GAGTC 4 cut(s) 93, 242, 617, 1216
PscI ACATGT 1 cut(s) 1140
PshBI ATTAAT 3 cut(s) 623, 639, 683
PsiI TTATAA 1 cut(s) 395
PspFI CCCAGC 1 cut(s) 749
PsrI GAACNNNNNNTAC 2 cut(s) 937, 969
PsyI GACNNNGTC 1 cut(s) 54
RsaI GTAC 4 cut(s) 182, 333, 850, 1233
RsaNI GTAC 4 cut(s) 181, 332, 849, 1232
RseI CAYNNNNRTG 2 cut(s) 200, 887
SaqAI TTAA 6 cut(s) 258, 623, 639, 683, 858, 1217
SatI GCNGC 3 cut(s) 238, 765, 960
Sau3AI GATC 3 cut(s) 165, 940, 1192
SchI GAGTC 4 cut(s) 93, 242, 618, 1217
ScrFI CCNGG 1 cut(s) 285
SfaNI GCATC 6 cut(s) 69, 355, 751, 805, 958, 1074
SfcI CTRYAG 1 cut(s) 844
SmiMI CAYNNNNRTG 2 cut(s) 200, 887
SmlI CTYRAG 3 cut(s) 92, 257, 857
SmoI CTYRAG 3 cut(s) 92, 257, 857
Sse9I AATT 6 cut(s) 358, 640, 680, 806, 979, 1019
SseBI AGGCCT 1 cut(s) 648
SsiI CCGC 8 cut(s) 69, 79, 109, 212, 237, 408, 459, 765
SspMI CTAG 3 cut(s) 171, 692, 1148
StuI AGGCCT 1 cut(s) 648
StyD4I CCNGG 1 cut(s) 283
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 5 cut(s) 180, 307, 567, 1120, 1127
TaiI ACGT 1 cut(s) 691
TaqI TCGA 5 cut(s) 246, 267, 423, 583, 943
TasI AATT 6 cut(s) 358, 640, 680, 806, 979, 1019
TatI WGTACW 1 cut(s) 848
TauI GCSGC 2 cut(s) 240, 767
TfiI GAWTC 4 cut(s) 420, 446, 872, 1177
Tru1I TTAA 6 cut(s) 258, 623, 639, 683, 858, 1217
Tru9I TTAA 6 cut(s) 258, 623, 639, 683, 858, 1217
TseFI GTSAC 2 cut(s) 307, 983
TseI GCWGC 1 cut(s) 959
Tsp45I GTSAC 2 cut(s) 307, 983
TspGWI ACGGA 1 cut(s) 439
Tth111I GACNNNGTC 1 cut(s) 54
Van91I CCANNNNNTGG 1 cut(s) 151
Vha464I CTTAAG 2 cut(s) 257, 857
VspI ATTAAT 3 cut(s) 623, 639, 683
XagI CCTNNNNNAGG 1 cut(s) 653
XapI RAATTY 2 cut(s) 358, 806
XceI RCATGY 3 cut(s) 673, 741, 1144
XmiI GTMKAC 1 cut(s) 612
XspI CTAG 3 cut(s) 171, 692, 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.