Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0006463

Physical Location & Seq

Genomic Coordinates Reverse (-)

12390673 .. 12397336

Gene Structure 6664 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1131300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1995 bp

ATGGTTATAACAGGCAAAGCAGTTGAATCAAAACGGGAAACTGAAAGGCCTAAGGTCTGGCGTTGTTCCATTTGCACATATGATAATGAAGAGAGTATGTCTGCATGTGACATTTGTGGGGTTCTTCGTAACCCTTTGTCTAAAAGCAATAGTGATAATAAAACAGCTTCTTTCAAGTTTGATGTTCCATCCCCAGATGATGTGGTTTCTAATGGATTGTGGTCCTCCAGAACAGGTTCAAGAGGTGGTGTTATTGACTTAAAATCTTCAAAAGCTTCCCCGAATGTGGCTAAGAAGAATAGTGCACTTAGCATACAATCAAATGCTGAACGGTCAGGTAGCTCATCTACAATGGCACAAACAAAGGAACAAGATAGATTAGATGAGGAAAATGGGTTGGAGAATCAGACAGTTTGTTATCACCATGGAAGTAGTGGCTCTATTACTGAACAAGAAAATGGTAAATTTTCAGAGAGTTCATCTGCTTCGCAGCCAACAAGCAGACGTGACAGTGGGGTTCAGAGCACTACTTCTTCTGAAATTATGGGCAAGCCACAGAGTGTTACCAGTGGCTTGATAAACATGACTTTGGATGCTCGATCTGGATATTCTAATAATGGTAGTGTTAGAGAATCTCGTTCACAAGCGGACTATAAACCCGAGAAGTGGATGCTTCCTGACCAAGCTGTAGATACACTGACTCAACTGAATCTTGCAATAGTTGGTCATGTTGATTCTGGAAAATCAACTCTCTCAGGTAGGTTGCTTCACCTTTTGGGAAGGATATCTCGAAAAGAAATGCACAAATATGAGAAAGAGGCAAAGTTGCAGGGAAAGGGGTCCTTCGCATATGCTTGGGCATTGGATGAGAGTGCAGAAGAAAGGGAAAGAGGAATAACTATGACAGTGGCTGTTGCATATTTTGATTCCAGAAAATATCATGTTGTTGTGCTCGACTCTCCTGGCCATAAAGATTTTGTTCCAAACATGATATCTGGGGCAACACAAGCTGATGCTGCAATACTCCTCATAGATGCCTCAGTTGGTGCATTCGAAGCTGGTATGGATGGCGGCAAGGGACAGACAAGGGAGCATGCGCAACTTATTAGAAGTTTTGGTGTTGATCAAATTATAGTTGCTGTTAACAAGATGGATAATGTTGAGTACTCCAAGGATCGATTTGATTTGATCAAACAGACACTTGGAACATTTCTCCGTTCCTGTGGTTTCAGAGATTCTTTGGTGTCTTGGATACCATTGAGTGCCATGGAAAATCAGAATTTGGTAGCAGCTCCTTCTGATGTCCGTTTATCGTCTTGGTACCGTGGACCTTACCTGTTGGATGCAATTGATTCCCTCCAACCTCCTACACGAGAGTTTTCAAAGCCATTGCTGATGCCTATATGTGATGTTATCAAATCTTCCTCACAAGGGCAGGTGTCTGCTTGTGGTAAATTGGAAGCGGGAGCTCTTCGAAGTGGATTTAAGGTTCTGGTGATGCCTTCAGGAGAATCAGGGACTGTACGTGCGTTAGAACGTGACTCTCAGGCTTGTGCAATTGCAAGAGCTGGAGACAATGTTGCTGTTACTCTGCAAGGCATTGATGGAGGTCGTGTGATGGCAGGAGGTGTGCTCTGTCACCCTAGTTTTCCAGTTGCAGTTGCAAAACATTTGGAACTAAAGGTGCTTGTTTTGGATGTTACAACCCCGATTTTAATTGGCTCTCAGTTGGAATTTCACATACACCACACGAAGGAGGCTGCGAGAGTTGTAAAAATATCATCATTGCTTGATCCAAAGACTGGCAAGGTGACAAGAAAAGCTCCTCGCTGTCTTACTGCAAAGCAGAGCGCAGTCGTAGAGGTAATTCTTCACGCGCCAGTTTGTGTGGAAGAGTTTTCTAATTCTAGAGCGCTGGGGAGGGCATTTTTAAGGGCATTAGGAAGCACTATTGCCGTCGGCGTTGTAACCCGGATTATAGAGGAGCAGAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000288 GO:0000291 GO:0000956 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019222 GO:0019439 GO:0019538 GO:0023052 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0034641 GO:0034645 GO:0034655 GO:0035639 GO:0036094 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043603 GO:0043604 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0090304 GO:0097159 GO:0097367 GO:1901265 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901566 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03015 ko05134 map03015 map05134

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

665

Amino Acids

71.74

Weight (kDa)

8.32

Isoelectric Point (pI)

48.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	236 - 454	8.2e-49	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	483 - 546	8.4e-07	Elongation factor Tu domain 2
GTP_EFTU_D3	PF03143	552 - 659	3.7e-11	Elongation factor Tu C-terminal domain
GTP-eEF1A_C	PF22594	558 - 657	7e-27	GTP-eEF1A C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006463)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10630 AT5G10630 AT5G10630 AT5G10630 AT5G10630
fragaria_vesca	FvH4_2g38640 FvH4_5g19710
malus_domestica	MD08G1131300.v1.1
prunus_persica	Prupe.1G461200_v2.0.a1 Prupe.1G461200_v2.0.a1 Prupe.1G461200_v2.0.a1 Prupe.1G461200_v2.0.a1 Prupe.1G461200_v2.0.a1
pyrus_communis	pycom08g11000
rosa_chinensis	RchiOBHm_Chr6g0302681
rosa_laevigata	RLG00000011148
rosa_multiflora	Rmu_co8422569.1_g000001 Rmu_sc0023908.1_g000003 Rmu_sc0043029.1_g000001
rosa_roxburghii	Rroxscaffold_7G00165440
rosa_rugosa	Rorug06G0316100
rosa_samantha	Rh6AG429400 Rh6BG473100 Rh6CG441700 Rh6DG428500
rosa_wichuraiana	Rw6G037170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	8
AarI	CACCTGC	1 cut(s)	1426
Acc16I	TGCGCA	1 cut(s)	1098
Acc36I	ACCTGC	1 cut(s)	1426
Acc65I	GGTACC	1 cut(s)	1320
AccB1I	GGYRCC	1 cut(s)	1320
AccB7I	CCANNNNNTGG	1 cut(s)	1802
AccII	CGCG	1 cut(s)	1877
AciI	CCGC	3 cut(s)	647, 1071, 1463
AclWI	GGATC	2 cut(s)	1182, 1787
AcoI	YGGCCR	1 cut(s)	964
AcsI	RAATTY	3 cut(s)	464, 1279, 1733
AcuI	CTGAAG	1 cut(s)	1488
AdeI	CACNNNGTG	1 cut(s)	560
AfaI	GTAC	3 cut(s)	1166, 1322, 1524
AfeI	AGCGCT	1 cut(s)	1914
AfiI	CCNNNNNNNGG	5 cut(s)	286, 666, 1431, 1753, 1802
AgsI	TTSAA	5 cut(s)	26, 175, 240, 270, 1383
AjiI	CACGTC	1 cut(s)	506
AjnI	CCWGG	1 cut(s)	961
AloI	GAACNNNNNNTCC	2 cut(s)	1473, 1505
Alw21I	GWGCWC	5 cut(s)	307, 527, 954, 1471, 1635
Alw26I	GTCTC	1 cut(s)	1566
Alw44I	GTGCAC	1 cut(s)	303
AlwI	GGATC	2 cut(s)	1182, 1787
AlwNI	CAGNNNCTG	2 cut(s)	911, 1520
Ama87I	CYCGRG	1 cut(s)	659
Aor51HI	AGCGCT	1 cut(s)	1914
AoxI	GGCC	2 cut(s)	47, 964
ApaLI	GTGCAC	1 cut(s)	303
ApeKI	GCWGC	4 cut(s)	490, 1016, 1289, 1760
ApoI	RAATTY	3 cut(s)	464, 1279, 1733
Asp700I	GAANNNNTTC	1 cut(s)	235
Asp718I	GGTACC	1 cut(s)	1320
AspLEI	GCGC	4 cut(s)	1099, 1853, 1879, 1915
AspS9I	GGNCC	3 cut(s)	222, 840, 1328
AsuC2I	CCSGG	1 cut(s)	1972
AsuHPI	GGTGA	5 cut(s)	413, 761, 1507, 1631, 1822
AsuII	TTCGAA	2 cut(s)	1053, 1474
AvaI	CYCGRG	1 cut(s)	659
AvaII	GGWCC	3 cut(s)	222, 840, 1328
AxyI	CCTNAGG	1 cut(s)	51
BaeGI	GKGCMC	1 cut(s)	307
BalI	TGGCCA	1 cut(s)	966
BanI	GGYRCC	1 cut(s)	1320
BanII	GRGCYC	1 cut(s)	1471
BauI	CACGAG	1 cut(s)	1371
Bbv12I	GWGCWC	5 cut(s)	307, 527, 954, 1471, 1635
BbvI	GCAGC	4 cut(s)	502, 1003, 1301, 1747
BccI	CCATC	5 cut(s)	196, 1061, 1144, 1598, 1612
BceAI	ACGGC	1 cut(s)	1940
BciT130I	CCWGG	1 cut(s)	963
BciVI	GTATCC	1 cut(s)	1245
BclI	TGATCA	2 cut(s)	1123, 1188
BcnI	CCSGG	1 cut(s)	1972
BcoDI	GTCTC	1 cut(s)	1566
BfaI	CTAG	2 cut(s)	1644, 1908
BfmI	CTRYAG	1 cut(s)	687
BfoI	RGCGCY	1 cut(s)	1916
BfuAI	ACCTGC	1 cut(s)	1426
BfuI	GTATCC	1 cut(s)	1245
BisI	GCNGC	5 cut(s)	491, 1017, 1072, 1290, 1761
BlsI	GCNGC	5 cut(s)	492, 1018, 1073, 1291, 1762
BmcAI	AGTACT	1 cut(s)	1166
Bme1390I	CCNGG	2 cut(s)	963, 1972
Bme18I	GGWCC	3 cut(s)	222, 840, 1328
BmeT110I	CYCGRG	1 cut(s)	659
BmgBI	CACGTC	1 cut(s)	506
BmgT120I	GGNCC	3 cut(s)	222, 840, 1328
BmiI	GGNNCC	2 cut(s)	841, 1322
BmrFI	CCNGG	2 cut(s)	963, 1972
BmsI	GCATC	7 cut(s)	583, 660, 1003, 1024, 1333, 1386, 1488
BplI	GAGNNNNNCTC	2 cut(s)	1617, 1649
BpmI	CTGGAG	2 cut(s)	211, 1590
Bpu14I	TTCGAA	2 cut(s)	1053, 1474
BpuMI	CCSGG	1 cut(s)	1972
Bsa29I	ATCGAT	1 cut(s)	1177
BsaAI	YACGTR	1 cut(s)	1526
BsaJI	CCNNGG	4 cut(s)	424, 1170, 1266, 1324
BsaXI	ACNNNNNCTCC	5 cut(s)	1563, 1593, 1599, 1629, 1976
Bsc4I	CCNNNNNNNGG	5 cut(s)	286, 666, 1431, 1753, 1802
Bse1I	ACTGG	4 cut(s)	567, 1652, 1807, 1880
Bse21I	CCTNAGG	1 cut(s)	51
Bse3DI	GCAATG	2 cut(s)	1388, 1784
BseBI	CCWGG	1 cut(s)	963
BseCI	ATCGAT	1 cut(s)	1177
BseDI	CCNNGG	4 cut(s)	424, 1170, 1266, 1324
BseGI	GGATG	7 cut(s)	188, 598, 675, 871, 1072, 1348, 1702
BseLI	CCNNNNNNNGG	5 cut(s)	286, 666, 1431, 1753, 1802
BseMI	GCAATG	2 cut(s)	1388, 1784
BseMII	CTCAG	4 cut(s)	768, 1053, 1559, 1739
BseNI	ACTGG	4 cut(s)	567, 1652, 1807, 1880
BseRI	GAGGAG	2 cut(s)	1016, 1815
BseSI	GKGCMC	1 cut(s)	307
BseXI	GCAGC	4 cut(s)	502, 1003, 1301, 1747
BseYI	CCCAGC	1 cut(s)	1915
BsgI	GTGCAG	1 cut(s)	894
Bsh1236I	CGCG	1 cut(s)	1877
BshFI	GGCC	2 cut(s)	49, 966
BshNI	GGYRCC	1 cut(s)	1320
BshVI	ATCGAT	1 cut(s)	1177
BsiHKAI	GWGCWC	5 cut(s)	307, 527, 954, 1471, 1635
BsiHKCI	CYCGRG	1 cut(s)	659
BsiSI	CCGG	1 cut(s)	1972
BslFI	GGGAC	2 cut(s)	1092, 1531
BslI	CCNNNNNNNGG	5 cut(s)	286, 666, 1431, 1753, 1802
BsmAI	GTCTC	1 cut(s)	1566
BsmFI	GGGAC	2 cut(s)	1092, 1531
BsmI	GAATGC	1 cut(s)	1049
BsnI	GGCC	2 cut(s)	49, 966
BsoBI	CYCGRG	1 cut(s)	659
Bsp119I	TTCGAA	2 cut(s)	1053, 1474
Bsp1286I	GDGCHC	5 cut(s)	307, 527, 954, 1471, 1635
Bsp143I	GATC	5 cut(s)	599, 1123, 1174, 1188, 1792
Bsp19I	CCATGG	2 cut(s)	424, 1266
BspACI	CCGC	3 cut(s)	647, 1071, 1463
BspANI	GGCC	2 cut(s)	49, 966
BspCNI	CTCAG	4 cut(s)	767, 1052, 1558, 1738
BspDI	ATCGAT	1 cut(s)	1177
BspFNI	CGCG	1 cut(s)	1877
BspLI	GGNNCC	2 cut(s)	841, 1322
BspMI	ACCTGC	1 cut(s)	1426
BspPI	GGATC	2 cut(s)	1182, 1787
BspQI	GCTCTTC	1 cut(s)	1476
BspT104I	TTCGAA	2 cut(s)	1053, 1474
BspT107I	GGYRCC	1 cut(s)	1320
BsrDI	GCAATG	2 cut(s)	1388, 1784
BsrI	ACTGG	4 cut(s)	567, 1652, 1807, 1880
BssECI	CCNNGG	4 cut(s)	424, 1170, 1266, 1324
BssMI	GATC	5 cut(s)	599, 1123, 1174, 1188, 1792
BssSI	CACGAG	1 cut(s)	1371
BssT1I	CCWWGG	3 cut(s)	424, 1170, 1266
Bst2BI	CACGAG	1 cut(s)	1371
Bst2UI	CCWGG	1 cut(s)	963
Bst4CI	ACNGT	6 cut(s)	333, 412, 512, 907, 1325, 1522
Bst6I	CTCTTC	3 cut(s)	84, 1476, 1887
BstBAI	YACGTR	1 cut(s)	1526
BstBI	TTCGAA	2 cut(s)	1053, 1474
BstC8I	GCNNGC	2 cut(s)	551, 1095
BstDEI	CTNAG	7 cut(s)	51, 291, 308, 754, 1039, 1545, 1725
BstDSI	CCRYGG	3 cut(s)	424, 1266, 1324
BstF5I	GGATG	7 cut(s)	188, 598, 675, 871, 1072, 1348, 1702
BstFNI	CGCG	1 cut(s)	1877
BstH2I	RGCGCY	1 cut(s)	1916
BstHHI	GCGC	4 cut(s)	1099, 1853, 1879, 1915
BstKTI	GATC	5 cut(s)	602, 1126, 1177, 1191, 1795
BstMAI	GTCTC	1 cut(s)	1566
BstMBI	GATC	5 cut(s)	599, 1123, 1174, 1188, 1792
BstMWI	GCNNNNNNNGC	3 cut(s)	1007, 1016, 1055
BstNI	CCWGG	1 cut(s)	963
BstNSI	RCATGY	2 cut(s)	108, 1097
BstSCI	CCNGG	2 cut(s)	961, 1970
BstSFI	CTRYAG	1 cut(s)	687
BstSLI	GKGCMC	1 cut(s)	307
BstUI	CGCG	1 cut(s)	1877
BstV1I	GCAGC	4 cut(s)	502, 1003, 1301, 1747
Bsu15I	ATCGAT	1 cut(s)	1177
Bsu36I	CCTNAGG	1 cut(s)	51
BsuI	GTATCC	1 cut(s)	1245
BsuRI	GGCC	2 cut(s)	49, 966
BsuTUI	ATCGAT	1 cut(s)	1177
BtgI	CCRYGG	3 cut(s)	424, 1266, 1324
BtrI	CACGTC	1 cut(s)	506
BtsCI	GGATG	7 cut(s)	188, 598, 675, 871, 1072, 1348, 1702
BtsIMutI	CAGTG	4 cut(s)	517, 574, 695, 912
BveI	ACCTGC	1 cut(s)	1426
Cac8I	GCNNGC	2 cut(s)	551, 1095
CaiI	CAGNNNCTG	2 cut(s)	911, 1520
CfoI	GCGC	4 cut(s)	1099, 1853, 1879, 1915
Cfr13I	GGNCC	3 cut(s)	222, 840, 1328
ClaI	ATCGAT	1 cut(s)	1177
Csp6I	GTAC	3 cut(s)	1165, 1321, 1523
CviAII	CATG	8 cut(s)	105, 425, 583, 728, 941, 988, 1094, 1267
CviQI	GTAC	3 cut(s)	1165, 1321, 1523
DdeI	CTNAG	7 cut(s)	51, 291, 308, 754, 1039, 1545, 1725
DpnI	GATC	5 cut(s)	601, 1125, 1176, 1190, 1794
DpnII	GATC	5 cut(s)	599, 1123, 1174, 1188, 1792
DraIII	CACNNNGTG	1 cut(s)	560
EaeI	YGGCCR	1 cut(s)	964
Eam1104I	CTCTTC	3 cut(s)	84, 1476, 1887
EarI	CTCTTC	3 cut(s)	84, 1476, 1887
Ecl136II	GAGCTC	1 cut(s)	1469
Eco130I	CCWWGG	3 cut(s)	424, 1170, 1266
Eco147I	AGGCCT	1 cut(s)	49
Eco24I	GRGCYC	1 cut(s)	1471
Eco32I	GATATC	2 cut(s)	786, 993
Eco47I	GGWCC	3 cut(s)	222, 840, 1328
Eco47III	AGCGCT	1 cut(s)	1914
Eco53kI	GAGCTC	1 cut(s)	1469
Eco57I	CTGAAG	1 cut(s)	1488
Eco81I	CCTNAGG	1 cut(s)	51
Eco88I	CYCGRG	1 cut(s)	659
EcoICRI	GAGCTC	1 cut(s)	1469
EcoO109I	RGGNCCY	1 cut(s)	840
EcoRII	CCWGG	1 cut(s)	961
EcoRV	GATATC	2 cut(s)	786, 993
EcoT14I	CCWWGG	3 cut(s)	424, 1170, 1266
EcoT38I	GRGCYC	1 cut(s)	1471
ErhI	CCWWGG	3 cut(s)	424, 1170, 1266
FaeI	CATG	8 cut(s)	108, 428, 586, 731, 944, 991, 1097, 1270
FalI	AAGNNNNNCTT	2 cut(s)	827, 859
FaqI	GGGAC	2 cut(s)	1092, 1531
FatI	CATG	8 cut(s)	104, 424, 582, 727, 940, 987, 1093, 1266
FauI	CCCGC	1 cut(s)	1456
FauNDI	CATATG	2 cut(s)	79, 850
FbaI	TGATCA	2 cut(s)	1123, 1188
Fnu4HI	GCNGC	5 cut(s)	491, 1017, 1072, 1290, 1761
FokI	GGATG	7 cut(s)	175, 605, 682, 878, 1079, 1355, 1709
FriOI	GRGCYC	1 cut(s)	1471
Fsp4HI	GCNGC	5 cut(s)	491, 1017, 1072, 1290, 1761
FspBI	CTAG	2 cut(s)	1644, 1908
FspI	TGCGCA	1 cut(s)	1098
GlaI	GCGC	4 cut(s)	1098, 1852, 1878, 1914
GluI	GCNGC	5 cut(s)	491, 1017, 1072, 1290, 1761
GsaI	CCCAGC	1 cut(s)	1919
GsuI	CTGGAG	2 cut(s)	211, 1590
HaeII	RGCGCY	1 cut(s)	1916
HaeIII	GGCC	2 cut(s)	49, 966
HapII	CCGG	1 cut(s)	1972
HhaI	GCGC	4 cut(s)	1099, 1853, 1879, 1915
Hin1II	CATG	8 cut(s)	108, 428, 586, 731, 944, 991, 1097, 1270
Hin6I	GCGC	4 cut(s)	1097, 1851, 1877, 1913
HinP1I	GCGC	4 cut(s)	1097, 1851, 1877, 1913
HincII	GTYRAC	1 cut(s)	1144
HindII	GTYRAC	1 cut(s)	1144
HindIII	AAGCTT	1 cut(s)	273
HpaI	GTTAAC	1 cut(s)	1144
HpaII	CCGG	1 cut(s)	1972
HphI	GGTGA	5 cut(s)	413, 761, 1507, 1631, 1822
Hpy166II	GTNNAC	4 cut(s)	305, 641, 1144, 1328
Hpy188I	TCNGA	7 cut(s)	408, 472, 522, 538, 1232, 1278, 1300
Hpy188III	TCNNGA	9 cut(s)	228, 240, 603, 677, 738, 789, 930, 1506, 1908
Hpy8I	GTNNAC	4 cut(s)	305, 641, 1144, 1328
Hpy99I	CGWCG	1 cut(s)	1961
HpyAV	CCTTC	5 cut(s)	774, 853, 1305, 1512, 1747
HpyCH4III	ACNGT	6 cut(s)	333, 412, 512, 907, 1325, 1522
HpyCH4IV	ACGT	3 cut(s)	505, 1525, 1537
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1007, 1016, 1055
HpyF3I	CTNAG	7 cut(s)	51, 291, 308, 754, 1039, 1545, 1725
HpySE526I	ACGT	3 cut(s)	505, 1525, 1537
Hsp92II	CATG	8 cut(s)	108, 428, 586, 731, 944, 991, 1097, 1270
HspAI	GCGC	4 cut(s)	1097, 1851, 1877, 1913
KpnI	GGTACC	1 cut(s)	1324
Ksp22I	TGATCA	2 cut(s)	1123, 1188
KspAI	GTTAAC	1 cut(s)	1144
Kzo9I	GATC	5 cut(s)	599, 1123, 1174, 1188, 1792
LguI	GCTCTTC	1 cut(s)	1476
LmnI	GCTCC	5 cut(s)	1090, 1297, 1466, 1828, 1984
Lsp1109I	GCAGC	4 cut(s)	502, 1003, 1301, 1747
LweI	GCATC	7 cut(s)	583, 660, 1003, 1024, 1333, 1386, 1488
MaeI	CTAG	2 cut(s)	1644, 1908
MaeII	ACGT	3 cut(s)	505, 1525, 1537
MalI	GATC	5 cut(s)	601, 1125, 1176, 1190, 1794
MboI	GATC	5 cut(s)	599, 1123, 1174, 1188, 1792
MfeI	CAATTG	2 cut(s)	1347, 1557
MhlI	GDGCHC	5 cut(s)	307, 527, 954, 1471, 1635
MlsI	TGGCCA	1 cut(s)	966
MluNI	TGGCCA	1 cut(s)	966
MlyI	GAGTC	3 cut(s)	694, 950, 1535
MmeI	TCCRAC	4 cut(s)	378, 1320, 1384, 1710
Mox20I	TGGCCA	1 cut(s)	966
MroXI	GAANNNNTTC	1 cut(s)	235
MscI	TGGCCA	1 cut(s)	966
MseI	TTAA	5 cut(s)	260, 1143, 1485, 1715, 1931
MslI	CAYNNNNRTG	1 cut(s)	807
Msp20I	TGGCCA	1 cut(s)	966
MspI	CCGG	1 cut(s)	1972
MspR9I	CCNGG	2 cut(s)	963, 1972
MunI	CAATTG	2 cut(s)	1347, 1557
Mva1269I	GAATGC	1 cut(s)	1049
MvaI	CCWGG	1 cut(s)	963
MvnI	CGCG	1 cut(s)	1877
MwoI	GCNNNNNNNGC	3 cut(s)	1007, 1016, 1055
NciI	CCSGG	1 cut(s)	1972
NcoI	CCATGG	2 cut(s)	424, 1266
NdeI	CATATG	2 cut(s)	79, 850
NdeII	GATC	5 cut(s)	599, 1123, 1174, 1188, 1792
NlaIII	CATG	8 cut(s)	108, 428, 586, 731, 944, 991, 1097, 1270
NlaIV	GGNNCC	2 cut(s)	841, 1322
NmuCI	GTSAC	5 cut(s)	107, 506, 1538, 1637, 1810
NsbI	TGCGCA	1 cut(s)	1098
NspI	RCATGY	2 cut(s)	108, 1097
NspV	TTCGAA	2 cut(s)	1053, 1474
PaeI	GCATGC	1 cut(s)	1097
PaqCI	CACCTGC	1 cut(s)	1426
PceI	AGGCCT	1 cut(s)	49
PciSI	GCTCTTC	1 cut(s)	1476
PctI	GAATGC	1 cut(s)	1049
PdmI	GAANNNNTTC	1 cut(s)	235
PfeI	GAWTC	9 cut(s)	26, 403, 632, 709, 734, 926, 1235, 1352, 1511
PflMI	CCANNNNNTGG	1 cut(s)	1802
PkrI	GCNGC	5 cut(s)	492, 1018, 1073, 1291, 1762
PleI	GAGTC	3 cut(s)	694, 950, 1535
PpsI	GAGTC	3 cut(s)	694, 950, 1535
Ppu21I	YACGTR	1 cut(s)	1526
PpuMI	RGGWCCY	1 cut(s)	840
PsiI	TTATAA	1 cut(s)	8
Psp124BI	GAGCTC	1 cut(s)	1471
Psp5II	RGGWCCY	1 cut(s)	840
Psp6I	CCWGG	1 cut(s)	961
PspFI	CCCAGC	1 cut(s)	1915
PspGI	CCWGG	1 cut(s)	961
PspN4I	GGNNCC	2 cut(s)	841, 1322
PspPI	GGNCC	3 cut(s)	222, 840, 1328
PspPPI	RGGWCCY	1 cut(s)	840
PstNI	CAGNNNCTG	2 cut(s)	911, 1520
RsaI	GTAC	3 cut(s)	1166, 1322, 1524
RsaNI	GTAC	3 cut(s)	1165, 1321, 1523
RseI	CAYNNNNRTG	1 cut(s)	807
SacI	GAGCTC	1 cut(s)	1471
SapI	GCTCTTC	1 cut(s)	1476
SaqAI	TTAA	5 cut(s)	260, 1143, 1485, 1715, 1931
SatI	GCNGC	5 cut(s)	491, 1017, 1072, 1290, 1761
Sau3AI	GATC	5 cut(s)	599, 1123, 1174, 1188, 1792
Sau96I	GGNCC	3 cut(s)	222, 840, 1328
ScaI	AGTACT	1 cut(s)	1166
SchI	GAGTC	3 cut(s)	694, 950, 1535
ScrFI	CCNGG	2 cut(s)	963, 1972
SduI	GDGCHC	5 cut(s)	307, 527, 954, 1471, 1635
SfaNI	GCATC	7 cut(s)	583, 660, 1003, 1024, 1333, 1386, 1488
SfcI	CTRYAG	1 cut(s)	687
SfuI	TTCGAA	2 cut(s)	1053, 1474
SinI	GGWCC	3 cut(s)	222, 840, 1328
SmiMI	CAYNNNNRTG	1 cut(s)	807
SphI	GCATGC	1 cut(s)	1097
SseBI	AGGCCT	1 cut(s)	49
SsiI	CCGC	3 cut(s)	647, 1071, 1463
SspMI	CTAG	2 cut(s)	1644, 1908
SstI	GAGCTC	1 cut(s)	1471
StuI	AGGCCT	1 cut(s)	49
StyD4I	CCNGG	2 cut(s)	961, 1970
StyI	CCWWGG	3 cut(s)	424, 1170, 1266
TaaI	ACNGT	6 cut(s)	333, 412, 512, 907, 1325, 1522
TaiI	ACGT	3 cut(s)	508, 1528, 1540
TaqI	TCGA	6 cut(s)	598, 790, 954, 1053, 1177, 1474
TatI	WGTACW	1 cut(s)	1164
TauI	GCSGC	1 cut(s)	1074
TfiI	GAWTC	9 cut(s)	26, 403, 632, 709, 734, 926, 1235, 1352, 1511
Tru1I	TTAA	5 cut(s)	260, 1143, 1485, 1715, 1931
Tru9I	TTAA	5 cut(s)	260, 1143, 1485, 1715, 1931
TscAI	CASTG	4 cut(s)	517, 574, 702, 912
TseFI	GTSAC	5 cut(s)	107, 506, 1538, 1637, 1810
TseI	GCWGC	4 cut(s)	490, 1016, 1289, 1760
Tsp45I	GTSAC	5 cut(s)	107, 506, 1538, 1637, 1810
TspDTI	ATGAA	2 cut(s)	102, 468
TspGWI	ACGGA	2 cut(s)	1205, 1295
TspRI	CASTG	4 cut(s)	517, 574, 702, 912
Van91I	CCANNNNNTGG	1 cut(s)	1802
VneI	GTGCAC	1 cut(s)	303
VpaK11BI	GGWCC	3 cut(s)	222, 840, 1328
XapI	RAATTY	3 cut(s)	464, 1279, 1733
XbaI	TCTAGA	1 cut(s)	1907
XceI	RCATGY	2 cut(s)	108, 1097
XcmI	CCANNNNNNNNNTGG	1 cut(s)	431
XmnI	GAANNNNTTC	1 cut(s)	235
XspI	CTAG	2 cut(s)	1644, 1908
ZrmI	AGTACT	1 cut(s)	1166

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.