Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0010476

Physical Location & Seq

Genomic Coordinates Forward (+)

20910939 .. 20917157

Gene Structure 6219 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1173300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1464 bp

ATGTTATCCGGCCCAGAAACATACGGTCCTCTCACGGACCCGGCCCACGGCCTTTCAGAGTTAATCCCGCTTCTAATAATAACAAGTTGCCAACAATTCCAGAGCCCCTTTCGGCCGACCGACTTAATGGTTTCGATAGGAACACCGGCGAGTGCAGAGAATGGCTCTGCCACCCCTAATCCGGTCACCGCCGCATGCGACGCTGATAGAATGCTTCAGGCAGGCGATGGATACCATCCTTCGGCCAACGAAATAACCCCGGAGTCGGAATTTTTGAAGAAGCGAAAGTCGTCGATTCTTCCGTTGGAGGTCGGCACGCGCGTCATGTGCCGGTGGAGGGATAACAAGTACCACCCGGTCAAGGTCATTGAACGACGGAGAGTCCATTGCGGTGGGCCCAACGACTACGAGTATTACGTCCACTACACTGAGTTCAATAGGAGGCTTGACGAGTGGGTGAAACTTGACCAACTTGATCTTGATTCAGTGGAGGCTGTAGTTGATGAGAAAGTGGAGGACAAGGTTACAAGCTTGAAAATGACTCGTCATCAGAAGCGGAAGATTGATGAGACACATGTAGAGGGCCATGAGGAACTTGATGCTGCCAGCTTGCGTGAACACGAAGAGTTTACGAAAGTGAAAAATATAGCAACTATAGAACTTGGAAGATATGAAATTGAGACATGGTACTTCTCCCCCTTCCCACCCGAATATAATGACTCTTTGAAGCTGTACTTTTGTGAGTTTTGTCTCAATTTCATGAAGCGCAAAGAACAGCTTCAAAGGCATATGAGAAAATGTGATCTCAAGCATCCCCCTGGTGATGAGATATACCGAAGTGGTACACTTTCTATGTTTGAGGTTGATGGCAAAAAGAACAAGGTTTATGGGCAGAATCTTTGTTATTTGGCAAAATTGTTTCTTGATCACAAGACCCTGTATTATGATGTTGACCTCTTTCTGTTTTACATTTTATGTGAATGTGATGATCGGGGCTGCCATATGGTTGGATATTTTTCCAAGGAAAAGCATTCGGAGGAATCCTATAACTTGGCATGTATCCTCACCCTCCCTCCGTATCAAAGAAAAGGCTATGGAAAATTTTTGATTGCCTTCTCATATGAACTATCAAAGAAAGAAGGTAAAGTTGGCACGCCTGAAAGACCCCTCTCTGACCTAGGATTGCTGAGTTACAGAGGGTACTGGACCCGGGTGCTTCTAGATATCTTGAAAAAGCACAAGGGAAACATTTCGATCAAGGAGCTTAGTGACATGACAGCCATCAAGGCAGAGGATATATTGAACACCCTTCAGAGTCTAGAATTAATTCAGTACAGGAAGGGGCAGCATGTCATTTGTGCAGACCCAAAGGTCCTGGATCGTCATCTAAAAGCCGCCGGACGTGGGGGTCTCGAGGTCGATGTTAGCAAACTGATTTGGACGCCGTATAAAGAGCAAGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004402 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008080 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010224 GO:0010485 GO:0016043 GO:0016407 GO:0016410 GO:0016569 GO:0016570 GO:0016573 GO:0016740 GO:0016746 GO:0016747 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019538 GO:0033554 GO:0034212 GO:0034641 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043543 GO:0043967 GO:0043981 GO:0043995 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046483 GO:0048580 GO:0048583 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051276 GO:0051716 GO:0061733 GO:0065007 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901564 GO:2000026 GO:2000028 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MOZ_SAS Tudor-knot

Protein Analysis

488

Amino Acids

56.15

Weight (kDa)

6.4

Isoelectric Point (pI)

42.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tudor-knot	PF11717	102 - 160	1.5e-20	RNA binding activity-knot of a chromodomain
zf-MYST	PF17772	213 - 267	4.6e-25	MYST family zinc finger domain
MOZ_SAS	PF01853	272 - 449	2e-86	MOZ/SAS family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09740 AT5G09740 AT5G64610
fragaria_vesca	FvH4_5g38850
malus_domestica	MD08G1173300.v1.1 MD15G1358600.v1.1
prunus_persica	Prupe.1G506200_v2.0.a1 Prupe.1G506200_v2.0.a1
pyrus_communis	pycom08g14790 pycom15g32100
rosa_chinensis	RchiOBHm_Chr7g0240841
rosa_laevigata	RLG00000000621
rosa_multiflora	Rmu_co8495669.1_g000001
rosa_roxburghii	Rroxscaffold_3G00219580
rosa_rugosa	Rorug07G0339400
rosa_samantha	Rh7AG493800 Rh7BG465700 Rh7CG512200 Rh7DG478900
rosa_wichuraiana	Rw0G010710 Rw7G041780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1370, 1407
AccII	CGCG	2 cut(s)	319, 321
AciI	CCGC	6 cut(s)	68, 189, 192, 390, 556, 1395
AclWI	GGATC	1 cut(s)	1386
AcoI	YGGCCR	2 cut(s)	113, 243
AcsI	RAATTY	2 cut(s)	269, 1100
AcuI	CTGAAG	2 cut(s)	200, 1295
AcyI	GRCGYC	1 cut(s)	1442
AfaI	GTAC	6 cut(s)	350, 689, 734, 844, 1202, 1334
AfiI	CCNNNNNNNGG	7 cut(s)	47, 181, 241, 265, 337, 361, 1404
AflIII	ACRYGT	1 cut(s)	574
AgsI	TTSAA	8 cut(s)	277, 371, 436, 535, 727, 782, 1231, 1303
AhdI	GACNNNNNGTC	1 cut(s)	380
AjiI	CACGTC	1 cut(s)	1403
AjnI	CCWGG	2 cut(s)	817, 1374
AjuI	GAANNNNNNNTTGG	2 cut(s)	1131, 1163
AluBI	AGCT	5 cut(s)	531, 609, 730, 778, 1264
AluI	AGCT	5 cut(s)	531, 609, 730, 778, 1264
Alw26I	GTCTC	4 cut(s)	563, 674, 755, 1415
AlwI	GGATC	1 cut(s)	1386
Ama87I	CYCGRG	2 cut(s)	1209, 1412
AoxI	GGCC	7 cut(s)	10, 42, 49, 113, 243, 395, 583
ApaI	GGGCCC	1 cut(s)	399
ApeKI	GCWGC	3 cut(s)	602, 996, 1345
ApoI	RAATTY	2 cut(s)	269, 1100
AseI	ATTAAT	1 cut(s)	1325
Asp700I	GAANNNNTTC	3 cut(s)	777, 1249, 1326
AspA2I	CCTAGG	1 cut(s)	1177
AspLEI	GCGC	2 cut(s)	321, 768
AspS9I	GGNCC	9 cut(s)	11, 26, 37, 43, 395, 396, 583, 1206, 1372
AsuC2I	CCSGG	5 cut(s)	41, 260, 356, 1210, 1211
AsuHPI	GGTGA	4 cut(s)	178, 469, 833, 1057
AvaI	CYCGRG	2 cut(s)	1209, 1412
AvaII	GGWCC	4 cut(s)	26, 37, 1206, 1372
AvrII	CCTAGG	1 cut(s)	1177
BaeGI	GKGCMC	1 cut(s)	399
BanII	GRGCYC	2 cut(s)	107, 399
BbvI	GCAGC	3 cut(s)	589, 983, 1357
BccI	CCATC	4 cut(s)	221, 243, 860, 1289
BceAI	ACGGC	2 cut(s)	64, 1429
BciT130I	CCWGG	2 cut(s)	819, 1376
BciVI	GTATCC	2 cut(s)	224, 1070
BclI	TGATCA	1 cut(s)	925
BcnI	CCSGG	5 cut(s)	41, 260, 356, 1210, 1211
BcoDI	GTCTC	4 cut(s)	563, 674, 755, 1415
BfaI	CTAG	3 cut(s)	1178, 1220, 1319
BfmI	CTRYAG	2 cut(s)	495, 654
BfuI	GTATCC	2 cut(s)	224, 1070
BglI	GCCNNNNNGGC	1 cut(s)	1286
BisI	GCNGC	5 cut(s)	192, 603, 997, 1346, 1395
BlnI	CCTAGG	1 cut(s)	1177
BlsI	GCNGC	5 cut(s)	193, 604, 998, 1347, 1396
Bme1390I	CCNGG	7 cut(s)	41, 260, 356, 819, 1210, 1211, 1376
Bme18I	GGWCC	4 cut(s)	26, 37, 1206, 1372
BmeRI	GACNNNNNGTC	1 cut(s)	380
BmeT110I	CYCGRG	2 cut(s)	1209, 1412
BmgBI	CACGTC	1 cut(s)	1403
BmgT120I	GGNCC	9 cut(s)	11, 26, 37, 43, 395, 396, 583, 1206, 1372
BmiI	GGNNCC	3 cut(s)	39, 397, 1208
BmrFI	CCNGG	7 cut(s)	41, 260, 356, 819, 1210, 1211, 1376
BmsI	GCATC	2 cut(s)	589, 820
BplI	GAGNNNNNCTC	2 cut(s)	149, 181
BpuEI	CTTGAG	1 cut(s)	791
BpuMI	CCSGG	5 cut(s)	41, 260, 356, 1210, 1211
BsaBI	GATNNNNATC	1 cut(s)	1383
BsaHI	GRCGYC	1 cut(s)	1442
BsaI	GGTCTC	1 cut(s)	1415
BsaJI	CCNNGG	6 cut(s)	46, 258, 817, 1020, 1177, 1209
BsaWI	WCCGGW	1 cut(s)	181
BsaXI	ACNNNNNCTCC	2 cut(s)	1057, 1087
Bsc4I	CCNNNNNNNGG	7 cut(s)	47, 181, 241, 265, 337, 361, 1404
Bse118I	RCCGGY	2 cut(s)	145, 330
Bse1I	ACTGG	1 cut(s)	1208
Bse3DI	GCAATG	1 cut(s)	385
Bse8I	GATNNNNATC	1 cut(s)	1383
BseBI	CCWGG	2 cut(s)	819, 1376
BseDI	CCNNGG	6 cut(s)	46, 258, 817, 1020, 1177, 1209
BseGI	GGATG	2 cut(s)	235, 811
BseJI	GATNNNNATC	1 cut(s)	1383
BseLI	CCNNNNNNNGG	7 cut(s)	47, 181, 241, 265, 337, 361, 1404
BseMI	GCAATG	1 cut(s)	385
BseMII	CTCAG	2 cut(s)	420, 1178
BseNI	ACTGG	1 cut(s)	1208
BseSI	GKGCMC	1 cut(s)	399
BseX3I	CGGCCG	1 cut(s)	113
BseXI	GCAGC	3 cut(s)	589, 983, 1357
BsgI	GTGCAG	2 cut(s)	174, 1380
Bsh1236I	CGCG	2 cut(s)	319, 321
Bsh1285I	CGRYCG	2 cut(s)	116, 120
BshFI	GGCC	7 cut(s)	12, 44, 51, 115, 245, 397, 585
BsiEI	CGRYCG	2 cut(s)	116, 120
BsiHKCI	CYCGRG	2 cut(s)	1209, 1412
BsiSI	CCGG	9 cut(s)	9, 41, 146, 182, 260, 331, 356, 1210, 1398
BslI	CCNNNNNNNGG	7 cut(s)	47, 181, 241, 265, 337, 361, 1404
BsmAI	GTCTC	4 cut(s)	563, 674, 755, 1415
BsmI	GAATGC	2 cut(s)	216, 1030
BsnI	GGCC	7 cut(s)	12, 44, 51, 115, 245, 397, 585
Bso31I	GGTCTC	1 cut(s)	1415
BsoBI	CYCGRG	2 cut(s)	1209, 1412
Bsp120I	GGGCCC	1 cut(s)	395
Bsp1286I	GDGCHC	2 cut(s)	107, 399
Bsp143I	GATC	6 cut(s)	475, 802, 925, 988, 1254, 1378
BspACI	CCGC	6 cut(s)	68, 189, 192, 390, 556, 1395
BspANI	GGCC	7 cut(s)	12, 44, 51, 115, 245, 397, 585
BspCNI	CTCAG	2 cut(s)	421, 1179
BspFNI	CGCG	2 cut(s)	319, 321
BspHI	TCATGA	1 cut(s)	759
BspLI	GGNNCC	3 cut(s)	39, 397, 1208
BspPI	GGATC	1 cut(s)	1386
BspTNI	GGTCTC	1 cut(s)	1415
BsrDI	GCAATG	1 cut(s)	385
BsrFI	RCCGGY	2 cut(s)	145, 330
BsrI	ACTGG	1 cut(s)	1208
BssAI	RCCGGY	2 cut(s)	145, 330
BssECI	CCNNGG	6 cut(s)	46, 258, 817, 1020, 1177, 1209
BssMI	GATC	6 cut(s)	475, 802, 925, 988, 1254, 1378
BssNI	GRCGYC	1 cut(s)	1442
BssT1I	CCWWGG	2 cut(s)	1020, 1177
Bst2UI	CCWGG	2 cut(s)	819, 1376
Bst4CI	ACNGT	1 cut(s)	26
Bst6I	CTCTTC	1 cut(s)	618
BstACI	GRCGYC	1 cut(s)	1442
BstC8I	GCNNGC	6 cut(s)	196, 223, 317, 607, 611, 1154
BstDEI	CTNAG	3 cut(s)	429, 1187, 1265
BstDSI	CCRYGG	1 cut(s)	46
BstEII	GGTNACC	1 cut(s)	184
BstF5I	GGATG	2 cut(s)	235, 811
BstFNI	CGCG	2 cut(s)	319, 321
BstHHI	GCGC	2 cut(s)	321, 768
BstKTI	GATC	6 cut(s)	478, 805, 928, 991, 1257, 1381
BstMAI	GTCTC	4 cut(s)	563, 674, 755, 1415
BstMBI	GATC	6 cut(s)	475, 802, 925, 988, 1254, 1378
BstMCI	CGRYCG	2 cut(s)	116, 120
BstMWI	GCNNNNNNNGC	4 cut(s)	200, 327, 784, 1286
BstNI	CCWGG	2 cut(s)	819, 1376
BstNSI	RCATGY	4 cut(s)	198, 578, 1059, 1352
BstPI	GGTNACC	1 cut(s)	184
BstSCI	CCNGG	7 cut(s)	39, 258, 354, 817, 1208, 1209, 1374
BstSFI	CTRYAG	2 cut(s)	495, 654
BstSLI	GKGCMC	1 cut(s)	399
BstUI	CGCG	2 cut(s)	319, 321
BstV1I	GCAGC	3 cut(s)	589, 983, 1357
BstXI	CCANNNNNNTGG	2 cut(s)	392, 1007
BstZI	CGGCCG	1 cut(s)	113
BsuI	GTATCC	2 cut(s)	224, 1070
BsuRI	GGCC	7 cut(s)	12, 44, 51, 115, 245, 397, 585
BtgI	CCRYGG	1 cut(s)	46
BtgZI	GCGATG	1 cut(s)	240
BtrI	CACGTC	1 cut(s)	1403
BtsCI	GGATG	2 cut(s)	235, 811
BtsIMutI	CAGTG	2 cut(s)	426, 492
Cac8I	GCNNGC	6 cut(s)	196, 223, 317, 607, 611, 1154
CciI	TCATGA	1 cut(s)	759
CfoI	GCGC	2 cut(s)	321, 768
Cfr10I	RCCGGY	2 cut(s)	145, 330
Cfr13I	GGNCC	9 cut(s)	11, 26, 37, 43, 395, 396, 583, 1206, 1372
Cfr9I	CCCGGG	1 cut(s)	1209
CseI	GACGC	3 cut(s)	209, 310, 1450
Csp6I	GTAC	6 cut(s)	349, 688, 733, 843, 1201, 1333
CviAII	CATG	9 cut(s)	195, 325, 575, 587, 684, 760, 1056, 1273, 1349
CviQI	GTAC	6 cut(s)	349, 688, 733, 843, 1201, 1333
DdeI	CTNAG	3 cut(s)	429, 1187, 1265
DpnI	GATC	6 cut(s)	477, 804, 927, 990, 1256, 1380
DpnII	GATC	6 cut(s)	475, 802, 925, 988, 1254, 1378
DrdI	GACNNNNNNGTC	2 cut(s)	1370, 1407
DriI	GACNNNNNGTC	1 cut(s)	380
DseDI	GACNNNNNNGTC	2 cut(s)	1370, 1407
EaeI	YGGCCR	2 cut(s)	113, 243
EagI	CGGCCG	1 cut(s)	113
Eam1104I	CTCTTC	1 cut(s)	618
Eam1105I	GACNNNNNGTC	1 cut(s)	380
EarI	CTCTTC	1 cut(s)	618
EclXI	CGGCCG	1 cut(s)	113
Eco130I	CCWWGG	2 cut(s)	1020, 1177
Eco24I	GRGCYC	2 cut(s)	107, 399
Eco31I	GGTCTC	1 cut(s)	1415
Eco32I	GATATC	1 cut(s)	1225
Eco47I	GGWCC	4 cut(s)	26, 37, 1206, 1372
Eco52I	CGGCCG	1 cut(s)	113
Eco57I	CTGAAG	2 cut(s)	200, 1295
Eco88I	CYCGRG	2 cut(s)	1209, 1412
Eco91I	GGTNACC	1 cut(s)	184
EcoO109I	RGGNCCY	1 cut(s)	1372
EcoO65I	GGTNACC	1 cut(s)	184
EcoRII	CCWGG	2 cut(s)	817, 1374
EcoRV	GATATC	1 cut(s)	1225
EcoT14I	CCWWGG	2 cut(s)	1020, 1177
EcoT38I	GRGCYC	2 cut(s)	107, 399
ErhI	CCWWGG	2 cut(s)	1020, 1177
FaeI	CATG	9 cut(s)	198, 328, 578, 590, 687, 763, 1059, 1276, 1352
FalI	AAGNNNNNCTT	4 cut(s)	719, 751, 762, 794
FatI	CATG	9 cut(s)	194, 324, 574, 586, 683, 759, 1055, 1272, 1348
FauI	CCCGC	1 cut(s)	75
FauNDI	CATATG	3 cut(s)	789, 1002, 1120
FbaI	TGATCA	1 cut(s)	925
Fnu4HI	GCNGC	5 cut(s)	192, 603, 997, 1346, 1395
FokI	GGATG	2 cut(s)	222, 798
FriOI	GRGCYC	2 cut(s)	107, 399
Fsp4HI	GCNGC	5 cut(s)	192, 603, 997, 1346, 1395
FspBI	CTAG	3 cut(s)	1178, 1220, 1319
GlaI	GCGC	2 cut(s)	320, 767
GluI	GCNGC	5 cut(s)	192, 603, 997, 1346, 1395
HaeIII	GGCC	7 cut(s)	12, 44, 51, 115, 245, 397, 585
HapII	CCGG	9 cut(s)	9, 41, 146, 182, 260, 331, 356, 1210, 1398
HgaI	GACGC	3 cut(s)	209, 310, 1450
HhaI	GCGC	2 cut(s)	321, 768
Hin1I	GRCGYC	1 cut(s)	1442
Hin1II	CATG	9 cut(s)	198, 328, 578, 590, 687, 763, 1059, 1276, 1352
Hin6I	GCGC	2 cut(s)	319, 766
HinP1I	GCGC	2 cut(s)	319, 766
HincII	GTYRAC	1 cut(s)	952
HindII	GTYRAC	1 cut(s)	952
HindIII	AAGCTT	1 cut(s)	529
HinfI	GANTC	9 cut(s)	263, 295, 381, 482, 541, 719, 895, 1040, 1315
HpaII	CCGG	9 cut(s)	9, 41, 146, 182, 260, 331, 356, 1210, 1398
HphI	GGTGA	4 cut(s)	178, 469, 833, 1057
Hpy166II	GTNNAC	5 cut(s)	421, 617, 630, 845, 952
Hpy188I	TCNGA	6 cut(s)	58, 268, 552, 1036, 1174, 1314
Hpy188III	TCNNGA	8 cut(s)	100, 479, 760, 923, 1220, 1228, 1319, 1412
Hpy8I	GTNNAC	5 cut(s)	421, 617, 630, 845, 952
Hpy99I	CGWCG	3 cut(s)	203, 295, 378
HpyAV	CCTTC	6 cut(s)	249, 709, 1123, 1133, 1319, 1333
HpyCH4III	ACNGT	1 cut(s)	26
HpyCH4IV	ACGT	2 cut(s)	417, 1402
HpyCH4V	TGCA	2 cut(s)	155, 1361
HpyF10VI	GCNNNNNNNGC	4 cut(s)	200, 327, 784, 1286
HpyF3I	CTNAG	3 cut(s)	429, 1187, 1265
HpySE526I	ACGT	2 cut(s)	417, 1402
Hsp92I	GRCGYC	1 cut(s)	1442
Hsp92II	CATG	9 cut(s)	198, 328, 578, 590, 687, 763, 1059, 1276, 1352
HspAI	GCGC	2 cut(s)	319, 766
Ksp22I	TGATCA	1 cut(s)	925
Kzo9I	GATC	6 cut(s)	475, 802, 925, 988, 1254, 1378
LmnI	GCTCC	1 cut(s)	1261
Lsp1109I	GCAGC	3 cut(s)	589, 983, 1357
LweI	GCATC	2 cut(s)	589, 820
MaeI	CTAG	3 cut(s)	1178, 1220, 1319
MaeII	ACGT	2 cut(s)	417, 1402
MaeIII	GTNAC	4 cut(s)	184, 523, 1190, 1268
MalI	GATC	6 cut(s)	477, 804, 927, 990, 1256, 1380
MboI	GATC	6 cut(s)	475, 802, 925, 988, 1254, 1378
MboII	GAAGA	5 cut(s)	289, 290, 571, 635, 678
MhlI	GDGCHC	2 cut(s)	107, 399
MluCI	AATT	8 cut(s)	95, 269, 675, 754, 914, 1100, 1322, 1326
MlyI	GAGTC	5 cut(s)	272, 390, 535, 713, 1324
MmeI	TCCRAC	3 cut(s)	246, 285, 988
MroXI	GAANNNNTTC	3 cut(s)	777, 1249, 1326
MseI	TTAA	3 cut(s)	62, 125, 1325
MslI	CAYNNNNRTG	1 cut(s)	390
MspI	CCGG	9 cut(s)	9, 41, 146, 182, 260, 331, 356, 1210, 1398
MspR9I	CCNGG	7 cut(s)	41, 260, 356, 819, 1210, 1211, 1376
Mva1269I	GAATGC	2 cut(s)	216, 1030
MvaI	CCWGG	2 cut(s)	819, 1376
MvnI	CGCG	2 cut(s)	319, 321
MwoI	GCNNNNNNNGC	4 cut(s)	200, 327, 784, 1286
NciI	CCSGG	5 cut(s)	41, 260, 356, 1210, 1211
NdeI	CATATG	3 cut(s)	789, 1002, 1120
NdeII	GATC	6 cut(s)	475, 802, 925, 988, 1254, 1378
NlaIII	CATG	9 cut(s)	198, 328, 578, 590, 687, 763, 1059, 1276, 1352
NlaIV	GGNNCC	3 cut(s)	39, 397, 1208
NmuCI	GTSAC	2 cut(s)	184, 1268
NspI	RCATGY	4 cut(s)	198, 578, 1059, 1352
PaeI	GCATGC	1 cut(s)	198
PaeR7I	CTCGAG	1 cut(s)	1412
PagI	TCATGA	1 cut(s)	759
PciI	ACATGT	1 cut(s)	574
PcsI	WCGNNNNNNNCGW	3 cut(s)	299, 318, 414
PctI	GAATGC	2 cut(s)	216, 1030
PdmI	GAANNNNTTC	3 cut(s)	777, 1249, 1326
PfeI	GAWTC	4 cut(s)	295, 482, 895, 1040
PfoI	TCCNGGA	1 cut(s)	1374
PkrI	GCNGC	5 cut(s)	193, 604, 998, 1347, 1396
PleI	GAGTC	5 cut(s)	271, 389, 535, 713, 1323
PpsI	GAGTC	5 cut(s)	271, 389, 535, 713, 1323
PpuMI	RGGWCCY	1 cut(s)	1372
PscI	ACATGT	1 cut(s)	574
PshBI	ATTAAT	1 cut(s)	1325
Psp5II	RGGWCCY	1 cut(s)	1372
Psp6I	CCWGG	2 cut(s)	817, 1374
PspEI	GGTNACC	1 cut(s)	184
PspGI	CCWGG	2 cut(s)	817, 1374
PspN4I	GGNNCC	3 cut(s)	39, 397, 1208
PspOMI	GGGCCC	1 cut(s)	395
PspPI	GGNCC	9 cut(s)	11, 26, 37, 43, 395, 396, 583, 1206, 1372
PspPPI	RGGWCCY	1 cut(s)	1372
RsaI	GTAC	6 cut(s)	350, 689, 734, 844, 1202, 1334
RsaNI	GTAC	6 cut(s)	349, 688, 733, 843, 1201, 1333
RseI	CAYNNNNRTG	1 cut(s)	390
SaqAI	TTAA	3 cut(s)	62, 125, 1325
SatI	GCNGC	5 cut(s)	192, 603, 997, 1346, 1395
Sau3AI	GATC	6 cut(s)	475, 802, 925, 988, 1254, 1378
Sau96I	GGNCC	9 cut(s)	11, 26, 37, 43, 395, 396, 583, 1206, 1372
SchI	GAGTC	5 cut(s)	272, 390, 535, 713, 1324
ScrFI	CCNGG	7 cut(s)	41, 260, 356, 819, 1210, 1211, 1376
SduI	GDGCHC	2 cut(s)	107, 399
SfaNI	GCATC	2 cut(s)	589, 820
SfcI	CTRYAG	2 cut(s)	495, 654
Sfr274I	CTCGAG	1 cut(s)	1412
SgrAI	CRCCGGYG	1 cut(s)	145
SinI	GGWCC	4 cut(s)	26, 37, 1206, 1372
SlaI	CTCGAG	1 cut(s)	1412
SmaI	CCCGGG	1 cut(s)	1211
SmiMI	CAYNNNNRTG	1 cut(s)	390
SmlI	CTYRAG	2 cut(s)	806, 1412
SmoI	CTYRAG	2 cut(s)	806, 1412
SphI	GCATGC	1 cut(s)	198
Sse9I	AATT	8 cut(s)	95, 269, 675, 754, 914, 1100, 1322, 1326
SsiI	CCGC	6 cut(s)	68, 189, 192, 390, 556, 1395
SspMI	CTAG	3 cut(s)	1178, 1220, 1319
StyD4I	CCNGG	7 cut(s)	39, 258, 354, 817, 1208, 1209, 1374
StyI	CCWWGG	2 cut(s)	1020, 1177
TaaI	ACNGT	1 cut(s)	26
TaiI	ACGT	2 cut(s)	420, 1405
TaqI	TCGA	5 cut(s)	134, 293, 1253, 1413, 1419
TaqII	GACCGA	1 cut(s)	134
TasI	AATT	8 cut(s)	95, 269, 675, 754, 914, 1100, 1322, 1326
TatI	WGTACW	2 cut(s)	732, 1332
TauI	GCSGC	2 cut(s)	194, 1397
TfiI	GAWTC	4 cut(s)	295, 482, 895, 1040
Tru1I	TTAA	3 cut(s)	62, 125, 1325
Tru9I	TTAA	3 cut(s)	62, 125, 1325
TscAI	CASTG	2 cut(s)	433, 492
TseFI	GTSAC	2 cut(s)	184, 1268
TseI	GCWGC	3 cut(s)	602, 996, 1345
Tsp45I	GTSAC	2 cut(s)	184, 1268
TspDTI	ATGAA	4 cut(s)	687, 748, 776, 1137
TspGWI	ACGGA	4 cut(s)	50, 291, 391, 1065
TspMI	CCCGGG	1 cut(s)	1209
TspRI	CASTG	2 cut(s)	433, 492
VpaK11BI	GGWCC	4 cut(s)	26, 37, 1206, 1372
VspI	ATTAAT	1 cut(s)	1325
XapI	RAATTY	2 cut(s)	269, 1100
XbaI	TCTAGA	2 cut(s)	1219, 1318
XceI	RCATGY	4 cut(s)	198, 578, 1059, 1352
XhoI	CTCGAG	1 cut(s)	1412
XmaI	CCCGGG	1 cut(s)	1209
XmaJI	CCTAGG	1 cut(s)	1177
XmnI	GAANNNNTTC	3 cut(s)	777, 1249, 1326
XspI	CTAG	3 cut(s)	1178, 1220, 1319

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.