Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0016064

Physical Location & Seq

Genomic Coordinates Forward (+)

21478586 .. 21479411

Gene Structure 826 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1176900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 450 bp

ATGGGAATTCAAGGAACTTTGGAGTACTTATCAGATGTAAGTAGTGCCAAGAAAGGCAAGAAGAAGAAGCAAATGCAAACGGTAGCCGTCAAAATCAGGATGGACTGTGAAGGTTGTGCCCGCAAGGTCAAGAATGTCCTCTCCGGAGTAAAAGGTGCTAAATCTGTGGACGTCGACTTGAAGCAGCAGAAGGCAACTGTGACTGGATATGTTGAGGCAAAGAAAGTGTTGAAGGCAGCTCAGTCAACAAAGAAGAAGTGTGAGTTGTGGCCTTATGTTCCATACAATCTGGTGGCTCATCCTTACATTTCTCAGGCATATGACAAGAAGGCACCTCCTAATATGGTCAGGAAAGTTGCCGACACTTCAAACATCACTGAGACTGCCGTGGACGACCGCTACATAGTCATGTTCAGCGATGACAATCCTAATGCCTGCTCTGTTATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0008150 GO:0009987 GO:0019725 GO:0030001 GO:0030003 GO:0042592 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HMA

Protein Analysis

150

Amino Acids

16.45

Weight (kDa)

9.5

Isoelectric Point (pI)

16.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	31 - 85	3e-14	Heavy-metal-associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016064)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08570
fragaria_vesca	FvH4_5g38120
malus_domestica	MD08G1176900.v1.1
prunus_persica	Prupe.1G511200_v2.0.a1
pyrus_communis	pycom08g15170 pycom15g32530
rosa_chinensis	RchiOBHm_Chr7g0240091
rosa_laevigata	RLG00000000707
rosa_multiflora	Rmu_sc0005008.1_g000037
rosa_roxburghii	Rroxscaffold_3G00220450
rosa_rugosa	Rorug07G0333000
rosa_samantha	Rh7AG486400 Rh7BG459400 Rh7CG503500
rosa_wichuraiana	Rw7G040780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	174
AccB1I	GGYRCC	1 cut(s)	331
AccI	GTMKAC	1 cut(s)	174
AccIII	TCCGGA	1 cut(s)	143
AciI	CCGC	2 cut(s)	121, 397
AcsI	RAATTY	1 cut(s)	6
AcyI	GRCGYC	1 cut(s)	171
AfaI	GTAC	1 cut(s)	26
AgsI	TTSAA	4 cut(s)	11, 181, 232, 369
AluBI	AGCT	1 cut(s)	239
AluI	AGCT	1 cut(s)	239
Alw26I	GTCTC	1 cut(s)	374
Aor13HI	TCCGGA	1 cut(s)	143
AoxI	GGCC	1 cut(s)	269
ApeKI	GCWGC	2 cut(s)	184, 236
ApoI	RAATTY	1 cut(s)	6
BaeGI	GKGCMC	1 cut(s)	121
BanI	GGYRCC	1 cut(s)	331
BbvI	GCAGC	2 cut(s)	196, 248
BccI	CCATC	1 cut(s)	94
BceAI	ACGGC	2 cut(s)	71, 371
BcoDI	GTCTC	1 cut(s)	374
BisI	GCNGC	2 cut(s)	185, 237
BlsI	GCNGC	2 cut(s)	186, 238
BmcAI	AGTACT	1 cut(s)	26
BmiI	GGNNCC	1 cut(s)	333
BsaBI	GATNNNNATC	1 cut(s)	423
BsaHI	GRCGYC	1 cut(s)	171
BsaJI	CCNNGG	1 cut(s)	387
BsaWI	WCCGGW	1 cut(s)	143
Bse1I	ACTGG	1 cut(s)	208
Bse8I	GATNNNNATC	1 cut(s)	423
BseAI	TCCGGA	1 cut(s)	143
BseDI	CCNNGG	1 cut(s)	387
BseGI	GGATG	2 cut(s)	105, 298
BseJI	GATNNNNATC	1 cut(s)	423
BseMII	CTCAG	3 cut(s)	254, 326, 369
BseNI	ACTGG	1 cut(s)	208
BseSI	GKGCMC	1 cut(s)	121
BseXI	GCAGC	2 cut(s)	196, 248
Bsh1285I	CGRYCG	1 cut(s)	397
BshFI	GGCC	1 cut(s)	271
BshNI	GGYRCC	1 cut(s)	331
BsiEI	CGRYCG	1 cut(s)	397
BsiSI	CCGG	1 cut(s)	144
BsmAI	GTCTC	1 cut(s)	374
BsnI	GGCC	1 cut(s)	271
Bsp1286I	GDGCHC	1 cut(s)	121
Bsp13I	TCCGGA	1 cut(s)	143
BspACI	CCGC	2 cut(s)	121, 397
BspANI	GGCC	1 cut(s)	271
BspCNI	CTCAG	3 cut(s)	253, 325, 370
BspEI	TCCGGA	1 cut(s)	143
BspLI	GGNNCC	1 cut(s)	333
BspT107I	GGYRCC	1 cut(s)	331
BsrI	ACTGG	1 cut(s)	208
BssECI	CCNNGG	1 cut(s)	387
BssNI	GRCGYC	1 cut(s)	171
Bst4CI	ACNGT	3 cut(s)	82, 107, 199
BstACI	GRCGYC	1 cut(s)	171
BstC8I	GCNNGC	2 cut(s)	121, 436
BstDEI	CTNAG	3 cut(s)	240, 312, 378
BstDSI	CCRYGG	1 cut(s)	387
BstF5I	GGATG	2 cut(s)	105, 298
BstMAI	GTCTC	1 cut(s)	374
BstMCI	CGRYCG	1 cut(s)	397
BstSLI	GKGCMC	1 cut(s)	121
BstV1I	GCAGC	2 cut(s)	196, 248
BsuRI	GGCC	1 cut(s)	271
BtgI	CCRYGG	1 cut(s)	387
BtgZI	GCGATG	1 cut(s)	432
BtsCI	GGATG	2 cut(s)	105, 298
BtsIMutI	CAGTG	1 cut(s)	375
Cac8I	GCNNGC	2 cut(s)	121, 436
Csp6I	GTAC	1 cut(s)	25
CviAII	CATG	1 cut(s)	409
CviJI	RGCY	4 cut(s)	86, 239, 271, 296
CviKI_1	RGCY	4 cut(s)	86, 239, 271, 296
CviQI	GTAC	1 cut(s)	25
DdeI	CTNAG	3 cut(s)	240, 312, 378
EcoRI	GAATTC	1 cut(s)	6
FaeI	CATG	1 cut(s)	412
FaiI	YATR	9 cut(s)	210, 276, 283, 319, 321, 344, 404, 410, 446
FatI	CATG	1 cut(s)	408
FauI	CCCGC	1 cut(s)	128
FauNDI	CATATG	1 cut(s)	319
FblI	GTMKAC	1 cut(s)	174
Fnu4HI	GCNGC	2 cut(s)	185, 237
FokI	GGATG	2 cut(s)	112, 285
Fsp4HI	GCNGC	2 cut(s)	185, 237
GluI	GCNGC	2 cut(s)	185, 237
HaeIII	GGCC	1 cut(s)	271
HapII	CCGG	1 cut(s)	144
Hin1I	GRCGYC	1 cut(s)	171
Hin1II	CATG	1 cut(s)	412
HincII	GTYRAC	2 cut(s)	175, 246
HindII	GTYRAC	2 cut(s)	175, 246
HpaII	CCGG	1 cut(s)	144
Hpy166II	GTNNAC	4 cut(s)	169, 175, 246, 391
Hpy188I	TCNGA	1 cut(s)	34
Hpy188III	TCNNGA	4 cut(s)	97, 130, 144, 349
Hpy8I	GTNNAC	4 cut(s)	169, 175, 246, 391
Hpy99I	CGWCG	1 cut(s)	176
HpyAV	CCTTC	4 cut(s)	104, 184, 226, 322
HpyCH4III	ACNGT	3 cut(s)	82, 107, 199
HpyCH4IV	ACGT	1 cut(s)	171
HpyCH4V	TGCA	1 cut(s)	76
HpyF3I	CTNAG	3 cut(s)	240, 312, 378
HpySE526I	ACGT	1 cut(s)	171
Hsp92I	GRCGYC	1 cut(s)	171
Hsp92II	CATG	1 cut(s)	412
Kpn2I	TCCGGA	1 cut(s)	143
LpnPI	CCDG	6 cut(s)	82, 157, 189, 275, 299, 334
Lsp1109I	GCAGC	2 cut(s)	196, 248
MaeII	ACGT	1 cut(s)	171
MaeIII	GTNAC	1 cut(s)	199
MboII	GAAGA	3 cut(s)	73, 76, 265
MhlI	GDGCHC	1 cut(s)	121
MluCI	AATT	1 cut(s)	6
MnlI	CCTC	3 cut(s)	149, 208, 345
MroI	TCCGGA	1 cut(s)	143
MslI	CAYNNNNRTG	1 cut(s)	407
MspI	CCGG	1 cut(s)	144
NdeI	CATATG	1 cut(s)	319
NlaIII	CATG	1 cut(s)	412
NlaIV	GGNNCC	1 cut(s)	333
NmuCI	GTSAC	1 cut(s)	199
PkrI	GCNGC	2 cut(s)	186, 238
PspN4I	GGNNCC	1 cut(s)	333
RsaI	GTAC	1 cut(s)	26
RsaNI	GTAC	1 cut(s)	25
RseI	CAYNNNNRTG	1 cut(s)	407
SalI	GTCGAC	1 cut(s)	173
SatI	GCNGC	2 cut(s)	185, 237
ScaI	AGTACT	1 cut(s)	26
SduI	GDGCHC	1 cut(s)	121
SetI	ASST	6 cut(s)	115, 129, 157, 174, 241, 337
SmiMI	CAYNNNNRTG	1 cut(s)	407
Sse9I	AATT	1 cut(s)	6
SsiI	CCGC	2 cut(s)	121, 397
TaaI	ACNGT	3 cut(s)	82, 107, 199
TaiI	ACGT	1 cut(s)	174
TaqI	TCGA	1 cut(s)	174
TasI	AATT	1 cut(s)	6
TatI	WGTACW	1 cut(s)	24
TscAI	CASTG	1 cut(s)	382
TseFI	GTSAC	1 cut(s)	199
TseI	GCWGC	2 cut(s)	184, 236
Tsp45I	GTSAC	1 cut(s)	199
TspRI	CASTG	1 cut(s)	382
XapI	RAATTY	1 cut(s)	6
XmiI	GTMKAC	1 cut(s)	174
ZraI	GACGTC	1 cut(s)	172
ZrmI	AGTACT	1 cut(s)	26

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.