pycom08g15170

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0016064
Physical Location & Seq
Forward (+)
14639838 .. 14640526
689 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g15170.1

Sequence Viewer

Length: 456 bp
ATGGGAATTCAAGGAACTTTGGAGTACTTATCAGATGTACTAAGTAGTGCCAAAAAAGGCAAGAAGAAGAAGCAAATGCAAACGGTAGCCGTCAAAATCAGGATGGACTGTGAAGGTTGTGCCCGCAAGGTCAAGAATGTCCTCTCCGGCGTAAAAGGTGCTAAATCTGTGGACGTCGACTTGAAGCAGCAGAAGGCAACTGTGACTGGATATAATGTTGAGGCAAAGAAAGTGTTGAAGGCAGCTCAGTCAACAAAGAAGAAGTGTGAGTTGTGGCCTTATGTTCCGTACAATCTGGTGGCTCATCCTTACATTTCTCAGGCATATGACAAGAAGGCACCTCCTAATATGGTCAGGAAAGTTGCCGACACTTCAAACATCACTGAGACTGCCGTGGACGACCGCTACATAGTCATGTTCAGCGATGACAATCCTAATGCCTGCTCTGTTATGTAG

Protein Analysis

152

Amino Acids

16.68

Weight (kDa)

9.5

Isoelectric Point (pI)

16.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 32 - 87 2.4e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016064)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08570
fragaria_vesca FvH4_5g38120
malus_domestica MD08G1176900.v1.1
prunus_persica Prupe.1G511200_v2.0.a1
pyrus_communis pycom08g15170 pycom15g32530
rosa_chinensis RchiOBHm_Chr7g0240091
rosa_laevigata RLG00000000707
rosa_multiflora Rmu_sc0005008.1_g000037
rosa_roxburghii Rroxscaffold_3G00220450
rosa_rugosa Rorug07G0333000
rosa_samantha Rh7AG486400 Rh7BG459400 Rh7CG503500
rosa_wichuraiana Rw7G040780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 177
AccB1I GGYRCC 1 cut(s) 337
AccI GTMKAC 1 cut(s) 177
AciI CCGC 2 cut(s) 124, 403
AcsI RAATTY 1 cut(s) 6
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 3 cut(s) 26, 39, 290
AgsI TTSAA 4 cut(s) 11, 184, 238, 375
AluBI AGCT 1 cut(s) 245
AluI AGCT 1 cut(s) 245
Alw26I GTCTC 1 cut(s) 380
AoxI GGCC 1 cut(s) 275
ApeKI GCWGC 2 cut(s) 187, 242
ApoI RAATTY 1 cut(s) 6
BaeGI GKGCMC 1 cut(s) 124
BanI GGYRCC 1 cut(s) 337
BbvI GCAGC 2 cut(s) 199, 254
BccI CCATC 1 cut(s) 97
BceAI ACGGC 2 cut(s) 74, 377
BcoDI GTCTC 1 cut(s) 380
BisI GCNGC 2 cut(s) 188, 243
BlsI GCNGC 2 cut(s) 189, 244
BmcAI AGTACT 1 cut(s) 26
BmiI GGNNCC 1 cut(s) 339
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 393
Bse1I ACTGG 1 cut(s) 211
Bse8I GATNNNNATC 1 cut(s) 429
BseDI CCNNGG 1 cut(s) 393
BseGI GGATG 2 cut(s) 108, 304
BseJI GATNNNNATC 1 cut(s) 429
BseMII CTCAG 3 cut(s) 260, 332, 375
BseNI ACTGG 1 cut(s) 211
BseSI GKGCMC 1 cut(s) 124
BseXI GCAGC 2 cut(s) 199, 254
Bsh1285I CGRYCG 1 cut(s) 403
BshFI GGCC 1 cut(s) 277
BshNI GGYRCC 1 cut(s) 337
BsiEI CGRYCG 1 cut(s) 403
BsiSI CCGG 1 cut(s) 147
BsmAI GTCTC 1 cut(s) 380
BsnI GGCC 1 cut(s) 277
Bsp1286I GDGCHC 1 cut(s) 124
BspACI CCGC 2 cut(s) 124, 403
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 3 cut(s) 259, 331, 376
BspLI GGNNCC 1 cut(s) 339
BspT107I GGYRCC 1 cut(s) 337
BsrI ACTGG 1 cut(s) 211
BssECI CCNNGG 1 cut(s) 393
BssNI GRCGYC 1 cut(s) 174
Bst4CI ACNGT 3 cut(s) 85, 110, 202
BstACI GRCGYC 1 cut(s) 174
BstC8I GCNNGC 2 cut(s) 124, 442
BstDEI CTNAG 4 cut(s) 41, 246, 318, 384
BstDSI CCRYGG 1 cut(s) 393
BstF5I GGATG 2 cut(s) 108, 304
BstMAI GTCTC 1 cut(s) 380
BstMCI CGRYCG 1 cut(s) 403
BstSLI GKGCMC 1 cut(s) 124
BstV1I GCAGC 2 cut(s) 199, 254
BsuRI GGCC 1 cut(s) 277
BtgI CCRYGG 1 cut(s) 393
BtgZI GCGATG 1 cut(s) 438
BtsCI GGATG 2 cut(s) 108, 304
BtsIMutI CAGTG 1 cut(s) 381
Cac8I GCNNGC 2 cut(s) 124, 442
Csp6I GTAC 3 cut(s) 25, 38, 289
CviAII CATG 1 cut(s) 415
CviJI RGCY 4 cut(s) 89, 245, 277, 302
CviKI_1 RGCY 4 cut(s) 89, 245, 277, 302
CviQI GTAC 3 cut(s) 25, 38, 289
DdeI CTNAG 4 cut(s) 41, 246, 318, 384
EcoRI GAATTC 1 cut(s) 6
FaeI CATG 1 cut(s) 418
FaiI YATR 8 cut(s) 213, 282, 325, 327, 350, 410, 416, 452
FatI CATG 1 cut(s) 414
FauI CCCGC 1 cut(s) 131
FauNDI CATATG 1 cut(s) 325
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 2 cut(s) 188, 243
FokI GGATG 2 cut(s) 115, 291
Fsp4HI GCNGC 2 cut(s) 188, 243
GluI GCNGC 2 cut(s) 188, 243
HaeIII GGCC 1 cut(s) 277
HapII CCGG 1 cut(s) 147
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 1 cut(s) 418
HincII GTYRAC 2 cut(s) 178, 252
HindII GTYRAC 2 cut(s) 178, 252
HpaII CCGG 1 cut(s) 147
Hpy166II GTNNAC 4 cut(s) 172, 178, 252, 397
Hpy188I TCNGA 1 cut(s) 34
Hpy188III TCNNGA 3 cut(s) 100, 133, 355
Hpy8I GTNNAC 4 cut(s) 172, 178, 252, 397
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 4 cut(s) 107, 187, 232, 328
HpyCH4III ACNGT 3 cut(s) 85, 110, 202
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 1 cut(s) 79
HpyF3I CTNAG 4 cut(s) 41, 246, 318, 384
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 1 cut(s) 418
LpnPI CCDG 6 cut(s) 85, 160, 192, 281, 305, 340
Lsp1109I GCAGC 2 cut(s) 199, 254
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 1 cut(s) 202
MboII GAAGA 3 cut(s) 76, 79, 271
MhlI GDGCHC 1 cut(s) 124
MluCI AATT 1 cut(s) 6
MnlI CCTC 3 cut(s) 152, 214, 351
MslI CAYNNNNRTG 1 cut(s) 413
MspI CCGG 1 cut(s) 147
NdeI CATATG 1 cut(s) 325
NlaIII CATG 1 cut(s) 418
NlaIV GGNNCC 1 cut(s) 339
NmuCI GTSAC 1 cut(s) 202
PkrI GCNGC 2 cut(s) 189, 244
PspN4I GGNNCC 1 cut(s) 339
RsaI GTAC 3 cut(s) 26, 39, 290
RsaNI GTAC 3 cut(s) 25, 38, 289
RseI CAYNNNNRTG 1 cut(s) 413
SalI GTCGAC 1 cut(s) 176
SatI GCNGC 2 cut(s) 188, 243
ScaI AGTACT 1 cut(s) 26
SduI GDGCHC 1 cut(s) 124
SetI ASST 6 cut(s) 118, 132, 160, 177, 247, 343
SmiMI CAYNNNNRTG 1 cut(s) 413
Sse9I AATT 1 cut(s) 6
SsiI CCGC 2 cut(s) 124, 403
TaaI ACNGT 3 cut(s) 85, 110, 202
TaiI ACGT 1 cut(s) 177
TaqI TCGA 1 cut(s) 177
TasI AATT 1 cut(s) 6
TatI WGTACW 2 cut(s) 24, 37
TscAI CASTG 1 cut(s) 388
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 2 cut(s) 187, 242
Tsp45I GTSAC 1 cut(s) 202
TspGWI ACGGA 1 cut(s) 276
TspRI CASTG 1 cut(s) 388
XapI RAATTY 1 cut(s) 6
XmiI GTMKAC 1 cut(s) 177
ZraI GACGTC 1 cut(s) 175
ZrmI AGTACT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.