Rh7AG486400

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0016064
Physical Location & Seq
Reverse (-)
68641170 .. 68641753
584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG486400.1

Sequence Viewer

Length: 453 bp
ATGGGAATTCAAGGCACCTTGGAGTACTTGTCAGATCTACTAAGCAGTGCCAAAACCAAGAAGAAGAAGAAGCAAACACAGACCGTAGCCGTGAAAATTAGGATGGACTGCGAGGGTTGTGCTCGCAAGGTGAAGAAGGTCCTCTCCGGTGTAAAAGGGGCTAAATCTGTGGATATCGACTTGAAGCAGCAGAAGGCAACGGTGACTGGTTACGTCGATGCAAAGAAAGTGTTGAAGGCAGCTCAGTCGACCAAGAAGAAGTGCGAGCTGTGGCCTTATGTGCCATACACTCTGGTGGCTCATCCTTATGTTGCCGGAGCCTACGACAAGAAGGCGCCTCCAAATATGGTTAGGAGCCTCCCAAGCACTGCCACTATCACTGAGAGTGCTGTGGATGACCACTACACTCAAATGTTTAGTGAGGAGAACCCAAATGCTTGCTCTGTAATGTAA

Protein Analysis

150

Amino Acids

16.45

Weight (kDa)

9.5

Isoelectric Point (pI)

27.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 32 - 86 1.8e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016064)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08570
fragaria_vesca FvH4_5g38120
malus_domestica MD08G1176900.v1.1
prunus_persica Prupe.1G511200_v2.0.a1
pyrus_communis pycom08g15170 pycom15g32530
rosa_chinensis RchiOBHm_Chr7g0240091
rosa_laevigata RLG00000000707
rosa_multiflora Rmu_sc0005008.1_g000037
rosa_roxburghii Rroxscaffold_3G00220450
rosa_rugosa Rorug07G0333000
rosa_samantha Rh7AG486400 Rh7BG459400 Rh7CG503500
rosa_wichuraiana Rw7G040780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 14, 334
AccI GTMKAC 1 cut(s) 248
AcsI RAATTY 1 cut(s) 6
AcyI GRCGYC 1 cut(s) 335
AfaI GTAC 1 cut(s) 26
AgsI TTSAA 3 cut(s) 11, 184, 235
AleI CACNNNNGTG 1 cut(s) 293
AluBI AGCT 2 cut(s) 242, 268
AluI AGCT 2 cut(s) 242, 268
Alw21I GWGCWC 1 cut(s) 124
AoxI GGCC 1 cut(s) 272
ApeKI GCWGC 2 cut(s) 187, 239
ApoI RAATTY 1 cut(s) 6
AspLEI GCGC 1 cut(s) 337
AspS9I GGNCC 1 cut(s) 139
AsuHPI GGTGA 2 cut(s) 142, 214
AvaII GGWCC 1 cut(s) 139
BanI GGYRCC 2 cut(s) 14, 334
Bbv12I GWGCWC 1 cut(s) 124
BbvI GCAGC 2 cut(s) 199, 251
BccI CCATC 1 cut(s) 97
BceAI ACGGC 1 cut(s) 74
BcgI CGANNNNNNTGC 4 cut(s) 101, 135, 228, 262
BfoI RGCGCY 1 cut(s) 338
BglII AGATCT 1 cut(s) 34
BisI GCNGC 2 cut(s) 188, 240
BlsI GCNGC 2 cut(s) 189, 241
BmcAI AGTACT 1 cut(s) 26
Bme18I GGWCC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 139
BmiI GGNNCC 4 cut(s) 16, 319, 336, 356
BmsI GCATC 1 cut(s) 208
BsaHI GRCGYC 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 18
BsaWI WCCGGW 1 cut(s) 146
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bse1I ACTGG 1 cut(s) 211
BseDI CCNNGG 1 cut(s) 18
BseGI GGATG 3 cut(s) 108, 301, 400
BseMII CTCAG 2 cut(s) 257, 372
BseNI ACTGG 1 cut(s) 211
BseRI GAGGAG 1 cut(s) 437
BseXI GCAGC 2 cut(s) 199, 251
BshFI GGCC 1 cut(s) 274
BshNI GGYRCC 2 cut(s) 14, 334
BsiHKAI GWGCWC 1 cut(s) 124
BsiSI CCGG 2 cut(s) 147, 315
BsnI GGCC 1 cut(s) 274
Bsp1286I GDGCHC 1 cut(s) 124
Bsp143I GATC 1 cut(s) 34
BspANI GGCC 1 cut(s) 274
BspCNI CTCAG 2 cut(s) 256, 373
BspLI GGNNCC 4 cut(s) 16, 319, 336, 356
BspT107I GGYRCC 2 cut(s) 14, 334
BsrI ACTGG 1 cut(s) 211
BssECI CCNNGG 1 cut(s) 18
BssMI GATC 1 cut(s) 34
BssNI GRCGYC 1 cut(s) 335
BssT1I CCWWGG 1 cut(s) 18
Bst4CI ACNGT 2 cut(s) 85, 202
BstACI GRCGYC 1 cut(s) 335
BstC8I GCNNGC 3 cut(s) 124, 266, 439
BstDEI CTNAG 3 cut(s) 41, 243, 381
BstF5I GGATG 3 cut(s) 108, 301, 400
BstH2I RGCGCY 1 cut(s) 338
BstHHI GCGC 1 cut(s) 337
BstKTI GATC 1 cut(s) 37
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 2 cut(s) 280, 363
BstV1I GCAGC 2 cut(s) 199, 251
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BsuRI GGCC 1 cut(s) 274
BtsCI GGATG 3 cut(s) 108, 301, 400
BtsI GCAGTG 2 cut(s) 52, 366
BtsIMutI CAGTG 3 cut(s) 52, 366, 378
Cac8I GCNNGC 3 cut(s) 124, 266, 439
CfoI GCGC 1 cut(s) 337
Cfr13I GGNCC 1 cut(s) 139
Csp6I GTAC 1 cut(s) 25
CviJI RGCY 8 cut(s) 89, 161, 242, 268, 274, 299, 320, 357
CviKI_1 RGCY 8 cut(s) 89, 161, 242, 268, 274, 299, 320, 357
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 3 cut(s) 41, 243, 381
DinI GGCGCC 1 cut(s) 336
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 18
Eco32I GATATC 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 139
EcoO109I RGGNCCY 1 cut(s) 139
EcoRI GAATTC 1 cut(s) 6
EcoRV GATATC 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 18
EgeI GGCGCC 1 cut(s) 336
EheI GGCGCC 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 18
FaiI YATR 4 cut(s) 279, 286, 309, 347
FblI GTMKAC 1 cut(s) 248
Fnu4HI GCNGC 2 cut(s) 188, 240
FokI GGATG 3 cut(s) 115, 288, 407
Fsp4HI GCNGC 2 cut(s) 188, 240
GlaI GCGC 1 cut(s) 336
GluI GCNGC 2 cut(s) 188, 240
HaeII RGCGCY 1 cut(s) 338
HaeIII GGCC 1 cut(s) 274
HapII CCGG 2 cut(s) 147, 315
HhaI GCGC 1 cut(s) 337
Hin1I GRCGYC 1 cut(s) 335
Hin6I GCGC 1 cut(s) 335
HinP1I GCGC 1 cut(s) 335
HincII GTYRAC 1 cut(s) 249
HindII GTYRAC 1 cut(s) 249
HpaII CCGG 2 cut(s) 147, 315
HphI GGTGA 2 cut(s) 142, 214
Hpy166II GTNNAC 1 cut(s) 249
Hpy188I TCNGA 1 cut(s) 34
Hpy8I GTNNAC 1 cut(s) 249
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 4 cut(s) 130, 187, 229, 325
HpyCH4III ACNGT 2 cut(s) 85, 202
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 1 cut(s) 221
HpyF10VI GCNNNNNNNGC 2 cut(s) 280, 363
HpyF3I CTNAG 3 cut(s) 41, 243, 381
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 1 cut(s) 335
HspAI GCGC 1 cut(s) 335
KasI GGCGCC 1 cut(s) 334
Kzo9I GATC 1 cut(s) 34
LmnI GCTCC 2 cut(s) 317, 354
LpnPI CCDG 4 cut(s) 160, 192, 278, 328
Lsp1109I GCAGC 2 cut(s) 199, 251
LweI GCATC 1 cut(s) 208
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 2 cut(s) 202, 209
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MboII GAAGA 5 cut(s) 73, 76, 79, 145, 268
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 124
MluCI AATT 2 cut(s) 6, 96
Mly113I GGCGCC 1 cut(s) 335
MnlI CCTC 5 cut(s) 106, 152, 348, 368, 415
MslI CAYNNNNRTG 3 cut(s) 293, 306, 410
MspI CCGG 2 cut(s) 147, 315
MwoI GCNNNNNNNGC 2 cut(s) 280, 363
NarI GGCGCC 1 cut(s) 335
NdeII GATC 1 cut(s) 34
NlaIV GGNNCC 4 cut(s) 16, 319, 336, 356
NmuCI GTSAC 1 cut(s) 202
OliI CACNNNNGTG 1 cut(s) 293
PkrI GCNGC 2 cut(s) 189, 241
PluTI GGCGCC 1 cut(s) 338
PpuMI RGGWCCY 1 cut(s) 139
Psp5II RGGWCCY 1 cut(s) 139
PspN4I GGNNCC 4 cut(s) 16, 319, 336, 356
PspPI GGNCC 1 cut(s) 139
PspPPI RGGWCCY 1 cut(s) 139
PsuI RGATCY 1 cut(s) 34
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
RseI CAYNNNNRTG 3 cut(s) 293, 306, 410
SalI GTCGAC 1 cut(s) 247
SatI GCNGC 2 cut(s) 188, 240
Sau3AI GATC 1 cut(s) 34
Sau96I GGNCC 1 cut(s) 139
ScaI AGTACT 1 cut(s) 26
SduI GDGCHC 1 cut(s) 124
SetI ASST 6 cut(s) 20, 132, 141, 216, 244, 270
SfaNI GCATC 1 cut(s) 208
SfoI GGCGCC 1 cut(s) 336
SinI GGWCC 1 cut(s) 139
SmiMI CAYNNNNRTG 3 cut(s) 293, 306, 410
Sse9I AATT 2 cut(s) 6, 96
SspDI GGCGCC 1 cut(s) 334
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 2 cut(s) 85, 202
TaiI ACGT 1 cut(s) 216
TaqI TCGA 3 cut(s) 177, 216, 248
TasI AATT 2 cut(s) 6, 96
TatI WGTACW 1 cut(s) 24
TscAI CASTG 3 cut(s) 52, 373, 385
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 2 cut(s) 187, 239
Tsp45I GTSAC 1 cut(s) 202
TspRI CASTG 3 cut(s) 52, 373, 385
VpaK11BI GGWCC 1 cut(s) 139
XapI RAATTY 1 cut(s) 6
XmiI GTMKAC 1 cut(s) 248
ZrmI AGTACT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.