Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002382

Physical Location & Seq

Genomic Coordinates Forward (+)

26245512 .. 26251929

Gene Structure 6418 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1202900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3177 bp

ATGGCAGAGGCACAGCCCACCGATTCCCACGCCGAATTCCCTGGTTCTTGGTCTTGCAACGTTCTTGGCCCTGAAACACACCCCAAACCGGTCGGCAATGGCGATGACGACGAAGGCTCGACCATCTCCGCGCTCTGTGTGCCCGGTTCGAGATTGGTCCGAACCGGGCTTGTTCACTCCACTTGCTCAGATAGCATTGTGTTATTTGTGAACGCCGGAGGGGAGGCCGTGACGGAAAATATAAATCCCGGGCTTCGAGTGGAGCCCGACGGATTTTTCCAAGGAGGGGATGTTCTAAGAACTAATGAGACCATTGACGGCAGCGATGTGCCATCCGTTTATCAATCTGCTCGGTTTGGGGATTTTTCGTACCGGTTTGAGAACCTATCTCCCGGAGAGTATTTTGTGGATCTGCACTTTGCGGAAATTGTATACACGAATGGTCCGAAAGGAATGAGAGTGTTTGATGTCTTCATGCAGGAAGAGAAGGTTCTATCAGGACTTGATATCTATTCTATCGTTGGAGCTAATAAGGCGCTACAGTTGGTAGATGTTGCAGTTTCGGTGGGGGAGGATGGGGCAATTTTGATAAGGTTTGATGGACTTAATGGAAGTCCAATTGTTGGGGGGATTTGTATAAAGCGAGCAGCTTCATTACCTGCATCAAGGATGAAACTTGGACATCTTGTATGTAACAATTGTGCCGCTGAGATAGAAATTTCATCTGCTCAGGACAAATATATAAGGTTGGAATTTACAACCAAGTATGAAAAGAAGATAGAGGATCTAAAGAGACGGTTCCAACTCAAGACAGATGAATGCCATGAGGCCTGGATGTCACTGACATCTGCAAACAAACAGTTACAGAACATCACCATGGAACTTGATAACAAGTTGTATGAAAGTCATTGCTTAGATCAAGCTAGAGAAGAAGCGGCAGCAAAATTGAGAGATATTTCTACTAGATATGAGCATGATAAGGGCTTGTGGACCGCAGTTGCTGACAATTTAGAAAGTAAAGTCAAGTTGATGAAGGAAGACTATGCTCAACTCCATTCTGAAGTGCACAAATTTACTGATGTTATTCCAGAATTCAACAATATGGTATCTGCAATTCAAGCAATGGTTACACAGTCTGATGAACTTAAGCTTAAGTTCAACGAGGAGCAGGCAAAGAGGAAAATACTTTATAACCAAATGCAGGAGGCAAAAGGGAACATTCGGGTGTTTTGTCGGTGTCGCCCGTTGAGGAAAGAGGAGTCATTAGCTGGGCATGCAACAGTTGTAAAATTTGATGCAGCTAAGGATGGAGAGCTTGGGATACTTACTAGTGGCTCCACTAAAAAGATATTCAGGTTTGACAGAGTTTACACACCAGAGGATGATCAAGTTGATGTATTTGCAGATGCTTCACCAATGATAACCTCAGTGTTAGATGGTTATAATGTTTGCATATTTGCCTATGGGCAAACGGGGACTGGAAAGACTTTCACAATGGAAGGTTCTGAGACCAACCGAGGGGTCAATTACAGGACCTTGGAACAGTTGTTTAAAACCGCTGAGGAAAGGAAAGAAATTTTTACGTACGGCATATCTGTTAGTGTGCTTGAAGTCTACAATGAGCAAATCAGGGACTTGCTAGCAGCCTCTCCGACTTCGAAAAGATTGGAAATGAGGCAAGCTCCTGAAGGAGCTCATCACATACCTGGAATTGTTGAGGCCAAAGTTGAGAGCATTAAAGAAGTTTGGAATGTTCTGCAAGCTGGAAGCAGTGCAAGAGCAGTTGGATCAAATAATGTGAACGAGCATAGCAGCCGATCTCATTGCATACTCTGCATCATGGTAAAAGCCAAGAATTTGATCACTGGTGAGTCCACTGTGAGCAAGCTTTGGCTTGTGGATTTGGCTGGCAGTGAGAGGCTAGCAAAAACAGAAGTTCAAGGGGAACGCCTTAAGGAAGCTCAAAACATCAATAGATCTTTGTCAGCTCTTGGGGATGTCATCTCTGCTTTGGCAACAAAAAAGAGCCACATTCCGTACAGGAATTCCAAGCTCACACATCTACTTCAAGACTCCTTGGGTGGTGATGCTAAAACTCTAATGTTTGTCCAAATAAGCCCTTCTGAGCAGGATGTAAGTGAGACTTTAAGTTCTCTGAACTTCGCAACACGGGTTCGAGGTGTCGAATTGGGTCCTGCAAGGAAGCAAATAGATACAGGCGAGGTTCAAAAGCTAAAACTAACACTTGACAAGCTAAAGCAAGAGTTGAAATCCAAAGATGATGATTTACATAAATTAGAAGAGAACTTTCAGAATCTAGAAGGTAAGTCAAAACGGGAGTTCCAACTGTCTAAAAGTCGACAAGAAAAGATTAATGAACTTGAAGACCAGGTTGCTGTGAAGACCGATGTGTGCAAACGACTGGAAAATCAGTTGTTGGAACTTTCAGATGAAGCAAATGGAAAGAGAGAATCATGCTTGAATCTTCAGAAAAAGGTCAAAGAGCTTGAGGACAAGTTGAAAGAACGAGAACGTGCAGATTCCATGATTCTTCAAAACAAGGTCAAGGACCTTGAGAACAAGTTAAAAGAAAGAACGCAAGAGTATGAGCACAATTCGGCTGTAGTTCAGCGGAAGGTTCAAGAACTCGAGAAAAGGCTGATAACGGAGGAAATCAACAAGGGATCTCTGCAACTGAAGGTTAAAGAGCTTGAAGAGAAGTTGAGACAGAAAGAACAAGAACATGTAATATCGACATTTTGTGGCGACAAGTCAGGTGCCACAACCCCCAATGGCAGAAAATCCTGGTCGAGAAGAGAGACCATTGGCGACATGGACCCTTTGAGGCTAAGTAACTTGAGCTATAGTAACCAAATGACAAGCCAGGAGTCTGTCCTACTCAAGGGAACCGACTCCCTCCGCGAGCTGAAGAGACGAAGAGAGTTGCACAGCAAAGGTCATGAGAATGTGATGTTGCCAGCTACTTTGGCTGATAGGAAGATGTCATCAGCCGAATCCAGCACTGACAAGTCTGGCGACCAAAAAGCACTGTCCAGGATCACAAGAAGTTTAACAAAACCGGCAACCACTGGTAAAAAAGCAGGCCCTTCGAACCCGAGCAACAAAAACCATAGAGATCAGGTTCCAGGTTCCAGGTCAAGGATTTGGTTAAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin Malectin

Protein Analysis

1059

Amino Acids

118.36

Weight (kDa)

6.59

Isoelectric Point (pI)

40.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin	PF11721	67 - 190	2e-17	Malectin domain
Microtub_bd	PF16796	387 - 547	1e-43	Microtubule binding
Kinesin	PF00225	412 - 727	1.6e-115	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22610 AT2G22610 AT2G22610
fragaria_vesca	FvH4_1g19311 FvH4_1g19311 FvH4_1g19311 FvH4_1g19311 FvH4_1g19311 FvH4_5g34300 FvH4_5g34300
malus_domestica	MD08G1202900.v1.1 MD15G1388700.v1.1
prunus_persica	Prupe.1G535100_v2.0.a1 Prupe.6G205100_v2.0.a1 Prupe.6G205100_v2.0.a1 Prupe.6G205100_v2.0.a1 Prupe.6G205100_v2.0.a1 Prupe.6G205100_v2.0.a1 Prupe.6G205100_v2.0.a1
pyrus_communis	pycom08g17520 pycom15g34860
rosa_chinensis	RchiOBHm_Chr2g0110681 RchiOBHm_Chr7g0234871
rosa_laevigata	RLG00000001208 RLG00000017830
rosa_multiflora	Rmu_sc0000039.1_g000002 Rmu_sc0000389.1_g000040 Rmu_sc0040758.1_g000001
rosa_roxburghii	Rroxscaffold_2G00133040 Rroxscaffold_3G00226690
rosa_rugosa	Rorug02G0168600 Rorug02G0168600 Rorug07G0285900
rosa_samantha	Rh2AG220900 Rh2BG231000 Rh2CG223300 Rh2DG227100 Rh7AG440500 Rh7BG413300 Rh7CG462200 Rh7DG430200
rosa_wichuraiana	Rw7G036620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1191, 1443
Acc36I	ACCTGC	1 cut(s)	667
AccB1I	GGYRCC	1 cut(s)	2777
AccB7I	CCANNNNNTGG	1 cut(s)	623
AccI	GTMKAC	3 cut(s)	432, 1614, 2359
AccII	CGCG	2 cut(s)	131, 2922
AciI	CCGC	8 cut(s)	129, 422, 705, 935, 993, 1557, 2632, 2920
AclI	AACGTT	1 cut(s)	60
AclWI	GGATC	5 cut(s)	417, 792, 1795, 2692, 3065
AcsI	RAATTY	9 cut(s)	35, 717, 752, 1070, 1091, 1289, 1575, 1855, 2044
AcuI	CTGAAG	5 cut(s)	1080, 1707, 2471, 2717, 2948
AfaI	GTAC	3 cut(s)	371, 1586, 2039
AfiI	CCNNNNNNNGG	7 cut(s)	88, 286, 623, 1201, 1518, 2902, 3159
AflII	CTTAAG	3 cut(s)	1145, 1151, 1952
AflIII	ACRYGT	1 cut(s)	2743
AgeI	ACCGGT	2 cut(s)	88, 372
AhlI	ACTAGT	1 cut(s)	1328
AjnI	CCWGG	9 cut(s)	40, 830, 1705, 2388, 2804, 2883, 3053, 3145, 3152
AloI	GAACNNNNNNTCC	2 cut(s)	276, 308
Alw21I	GWGCWC	3 cut(s)	1068, 1696, 2613
Alw26I	GTCTC	7 cut(s)	302, 787, 1502, 2135, 2719, 2813, 2926
Alw44I	GTGCAC	1 cut(s)	1064
AlwI	GGATC	5 cut(s)	417, 792, 1795, 2692, 3065
AlwNI	CAGNNNCTG	1 cut(s)	1001
Ama87I	CYCGRG	3 cut(s)	248, 2648, 3115
AoxI	GGCC	5 cut(s)	67, 225, 828, 1719, 3103
ApaLI	GTGCAC	1 cut(s)	1064
ApeKI	GCWGC	6 cut(s)	321, 647, 938, 1298, 1643, 1812
ApoI	RAATTY	9 cut(s)	35, 717, 752, 1070, 1091, 1289, 1575, 1855, 2044
AseI	ATTAAT	1 cut(s)	2373
AsiGI	ACCGGT	2 cut(s)	88, 372
AspLEI	GCGC	2 cut(s)	133, 538
AspS9I	GGNCC	9 cut(s)	68, 157, 443, 990, 1533, 2192, 2569, 2836, 3104
AsuC2I	CCSGG	5 cut(s)	144, 166, 249, 250, 393
AsuHPI	GGTGA	4 cut(s)	865, 1404, 1880, 2096
AsuII	TTCGAA	2 cut(s)	1658, 3110
AsuNHI	GCTAGC	2 cut(s)	1639, 1921
AvaI	CYCGRG	3 cut(s)	248, 2648, 3115
AvaII	GGWCC	7 cut(s)	157, 443, 990, 1533, 2192, 2569, 2836
BaeGI	GKGCMC	2 cut(s)	144, 1068
BanI	GGYRCC	1 cut(s)	2777
BanII	GRGCYC	2 cut(s)	267, 1696
BbsI	GAAGAC	4 cut(s)	463, 1044, 2391, 2408
Bbv12I	GWGCWC	3 cut(s)	1068, 1696, 2613
BbvCI	CCTCAGC	1 cut(s)	1560
BbvI	GCAGC	6 cut(s)	333, 659, 950, 1310, 1655, 1824
BccI	CCATC	6 cut(s)	131, 340, 569, 593, 1301, 1430
BceAI	ACGGC	3 cut(s)	212, 334, 1603
BcgI	CGANNNNNNTGC	4 cut(s)	1806, 1840, 3090, 3124
BciT130I	CCWGG	9 cut(s)	42, 832, 1707, 2390, 2806, 2885, 3055, 3147, 3154
BciVI	GTATCC	1 cut(s)	1314
BclI	TGATCA	2 cut(s)	1384, 1860
BcnI	CCSGG	5 cut(s)	144, 166, 249, 250, 393
BcoDI	GTCTC	7 cut(s)	302, 787, 1502, 2135, 2719, 2813, 2926
BcuI	ACTAGT	1 cut(s)	1328
BfaI	CTAG	6 cut(s)	924, 963, 1329, 1640, 1922, 2318
BfmI	CTRYAG	3 cut(s)	539, 2622, 2863
BfoI	RGCGCY	1 cut(s)	539
BfrI	CTTAAG	3 cut(s)	1145, 1151, 1952
BfuAI	ACCTGC	1 cut(s)	667
BfuI	GTATCC	1 cut(s)	1314
BglII	AGATCT	1 cut(s)	1976
BisI	GCNGC	8 cut(s)	322, 648, 705, 936, 939, 1299, 1644, 1813
BlsI	GCNGC	8 cut(s)	323, 649, 706, 937, 940, 1300, 1645, 1814
Bme18I	GGWCC	7 cut(s)	157, 443, 990, 1533, 2192, 2569, 2836
BmeT110I	CYCGRG	3 cut(s)	248, 2648, 3115
BmgT120I	GGNCC	9 cut(s)	68, 157, 443, 990, 1533, 2192, 2569, 2836, 3104
BmiI	GGNNCC	9 cut(s)	264, 800, 1336, 2193, 2779, 2838, 2908, 3144, 3151
BmsI	GCATC	5 cut(s)	671, 1285, 1396, 1845, 2077
BmtI	GCTAGC	2 cut(s)	1643, 1925
BpiI	GAAGAC	4 cut(s)	463, 1044, 2391, 2408
BplI	GAGNNNNNCTC	2 cut(s)	1666, 1698
Bpu10I	CCTNAGC	3 cut(s)	729, 1302, 1560
Bpu14I	TTCGAA	2 cut(s)	1658, 3110
BpuEI	CTTGAG	5 cut(s)	791, 2528, 2594, 2878, 2885
BpuMI	CCSGG	5 cut(s)	144, 166, 249, 250, 393
BsaAI	YACGTR	1 cut(s)	1584
BsaI	GGTCTC	3 cut(s)	302, 1502, 2813
BsaJI	CCNNGG	7 cut(s)	40, 248, 280, 876, 1516, 1536, 2076
BsaWI	WCCGGW	2 cut(s)	88, 372
BsaXI	ACNNNNNCTCC	2 cut(s)	276, 306
Bsc4I	CCNNNNNNNGG	7 cut(s)	88, 286, 623, 1201, 1518, 2902, 3159
Bse118I	RCCGGY	3 cut(s)	88, 372, 3079
Bse1I	ACTGG	4 cut(s)	1483, 1870, 2427, 3094
Bse3DI	GCAATG	4 cut(s)	103, 907, 1128, 1822
BseBI	CCWGG	9 cut(s)	42, 832, 1707, 2390, 2806, 2885, 3055, 3147, 3154
BseDI	CCNNGG	7 cut(s)	40, 248, 280, 876, 1516, 1536, 2076
BseGI	GGATG	9 cut(s)	295, 332, 580, 675, 840, 1312, 1387, 2002, 2137
BseLI	CCNNNNNNNGG	7 cut(s)	88, 286, 623, 1201, 1518, 2902, 3159
BseMI	GCAATG	4 cut(s)	103, 907, 1128, 1822
BseMII	CTCAG	7 cut(s)	201, 699, 743, 1440, 1497, 1551, 2115
BseNI	ACTGG	4 cut(s)	1483, 1870, 2427, 3094
BseRI	GAGGAG	2 cut(s)	1178, 1271
BseSI	GKGCMC	2 cut(s)	144, 1068
BseXI	GCAGC	6 cut(s)	333, 659, 950, 1310, 1655, 1824
BseYI	CCCAGC	1 cut(s)	1268
BsgI	GTGCAG	2 cut(s)	398, 2556
Bsh1236I	CGCG	2 cut(s)	131, 2922
Bsh1285I	CGRYCG	1 cut(s)	93
BshFI	GGCC	5 cut(s)	69, 227, 830, 1721, 3105
BshNI	GGYRCC	1 cut(s)	2777
BshTI	ACCGGT	2 cut(s)	88, 372
BsiEI	CGRYCG	1 cut(s)	93
BsiHKAI	GWGCWC	3 cut(s)	1068, 1696, 2613
BsiHKCI	CYCGRG	3 cut(s)	248, 2648, 3115
BsiSI	CCGG	8 cut(s)	89, 144, 165, 216, 249, 373, 393, 3080
BsiWI	CGTACG	1 cut(s)	1584
BslFI	GGGAC	2 cut(s)	1489, 1646
BslI	CCNNNNNNNGG	7 cut(s)	88, 286, 623, 1201, 1518, 2902, 3159
BsmAI	GTCTC	7 cut(s)	302, 787, 1502, 2135, 2719, 2813, 2926
BsmBI	CGTCTC	2 cut(s)	787, 2926
BsmFI	GGGAC	2 cut(s)	1489, 1646
BsmI	GAATGC	1 cut(s)	824
BsnI	GGCC	5 cut(s)	69, 227, 830, 1721, 3105
Bso31I	GGTCTC	3 cut(s)	302, 1502, 2813
BsoBI	CYCGRG	3 cut(s)	248, 2648, 3115
Bsp119I	TTCGAA	2 cut(s)	1658, 3110
Bsp1286I	GDGCHC	5 cut(s)	144, 267, 1068, 1696, 2613
Bsp19I	CCATGG	1 cut(s)	876
BspACI	CCGC	8 cut(s)	129, 422, 705, 935, 993, 1557, 2632, 2920
BspANI	GGCC	5 cut(s)	69, 227, 830, 1721, 3105
BspCNI	CTCAG	7 cut(s)	200, 700, 742, 1439, 1498, 1552, 2116
BspFNI	CGCG	2 cut(s)	131, 2922
BspHI	TCATGA	1 cut(s)	2959
BspLI	GGNNCC	9 cut(s)	264, 800, 1336, 2193, 2779, 2838, 2908, 3144, 3151
BspMI	ACCTGC	1 cut(s)	667
BspOI	GCTAGC	2 cut(s)	1643, 1925
BspPI	GGATC	5 cut(s)	417, 792, 1795, 2692, 3065
BspT104I	TTCGAA	2 cut(s)	1658, 3110
BspT107I	GGYRCC	1 cut(s)	2777
BspTI	CTTAAG	3 cut(s)	1145, 1151, 1952
BspTNI	GGTCTC	3 cut(s)	302, 1502, 2813
BsrDI	GCAATG	4 cut(s)	103, 907, 1128, 1822
BsrFI	RCCGGY	3 cut(s)	88, 372, 3079
BsrI	ACTGG	4 cut(s)	1483, 1870, 2427, 3094
BssAI	RCCGGY	3 cut(s)	88, 372, 3079
BssECI	CCNNGG	7 cut(s)	40, 248, 280, 876, 1516, 1536, 2076
BssNAI	GTATAC	1 cut(s)	433
BssT1I	CCWWGG	4 cut(s)	280, 876, 1536, 2076
Bst1107I	GTATAC	1 cut(s)	433
Bst2UI	CCWGG	9 cut(s)	42, 832, 1707, 2390, 2806, 2885, 3055, 3147, 3154
Bst4CI	ACNGT	9 cut(s)	543, 798, 861, 1134, 1282, 1545, 1879, 2349, 3051
Bst6I	CTCTTC	6 cut(s)	477, 2295, 2709, 2809, 2924, 2932
BstAFI	CTTAAG	3 cut(s)	1145, 1151, 1952
BstAPI	GCANNNNNTGC	1 cut(s)	1833
BstBAI	YACGTR	1 cut(s)	1584
BstBI	TTCGAA	2 cut(s)	1658, 3110
BstDSI	CCRYGG	1 cut(s)	876
BstENI	CCTNNNNNAGG	1 cut(s)	2900
BstF5I	GGATG	9 cut(s)	295, 332, 580, 675, 840, 1312, 1387, 2002, 2137
BstFNI	CGCG	2 cut(s)	131, 2922
BstH2I	RGCGCY	1 cut(s)	539
BstHHI	GCGC	2 cut(s)	133, 538
BstMAI	GTCTC	7 cut(s)	302, 787, 1502, 2135, 2719, 2813, 2926
BstMCI	CGRYCG	1 cut(s)	93
BstMWI	GCNNNNNNNGC	7 cut(s)	139, 192, 533, 1118, 1274, 1833, 2987
BstNI	CCWGG	9 cut(s)	42, 832, 1707, 2390, 2806, 2885, 3055, 3147, 3154
BstNSI	RCATGY	2 cut(s)	1277, 2747
BstSFI	CTRYAG	3 cut(s)	539, 2622, 2863
BstSLI	GKGCMC	2 cut(s)	144, 1068
BstSNI	TACGTA	1 cut(s)	1584
BstUI	CGCG	2 cut(s)	131, 2922
BstV1I	GCAGC	6 cut(s)	333, 659, 950, 1310, 1655, 1824
BstV2I	GAAGAC	4 cut(s)	463, 1044, 2391, 2408
BstX2I	RGATCY	4 cut(s)	409, 784, 1976, 2684
BstYI	RGATCY	4 cut(s)	409, 784, 1976, 2684
BstZ17I	GTATAC	1 cut(s)	433
BsuI	GTATCC	1 cut(s)	1314
BsuRI	GGCC	5 cut(s)	69, 227, 830, 1721, 3105
BtgI	CCRYGG	1 cut(s)	876
BtgZI	GCGATG	2 cut(s)	117, 339
BtsCI	GGATG	9 cut(s)	295, 332, 580, 675, 840, 1312, 1387, 2002, 2137
BtsI	GCAGTG	2 cut(s)	1777, 1918
BtsIMutI	CAGTG	9 cut(s)	839, 1434, 1777, 1863, 1875, 1918, 3021, 3047, 3087
BveI	ACCTGC	1 cut(s)	667
CaiI	CAGNNNCTG	1 cut(s)	1001
CciI	TCATGA	1 cut(s)	2959
CfoI	GCGC	2 cut(s)	133, 538
Cfr10I	RCCGGY	3 cut(s)	88, 372, 3079
Cfr13I	GGNCC	9 cut(s)	68, 157, 443, 990, 1533, 2192, 2569, 2836, 3104
Cfr9I	CCCGGG	1 cut(s)	248
CsiI	ACCWGGT	1 cut(s)	2388
Csp6I	GTAC	3 cut(s)	370, 1585, 2038
CspAI	ACCGGT	2 cut(s)	88, 372
CviQI	GTAC	3 cut(s)	370, 1585, 2038
DraI	TTTAAA	1 cut(s)	1552
Eam1104I	CTCTTC	6 cut(s)	477, 2295, 2709, 2809, 2924, 2932
EarI	CTCTTC	6 cut(s)	477, 2295, 2709, 2809, 2924, 2932
Ecl136II	GAGCTC	1 cut(s)	1694
Eco105I	TACGTA	1 cut(s)	1584
Eco130I	CCWWGG	4 cut(s)	280, 876, 1536, 2076
Eco147I	AGGCCT	1 cut(s)	830
Eco24I	GRGCYC	2 cut(s)	267, 1696
Eco31I	GGTCTC	3 cut(s)	302, 1502, 2813
Eco32I	GATATC	1 cut(s)	508
Eco47I	GGWCC	7 cut(s)	157, 443, 990, 1533, 2192, 2569, 2836
Eco53kI	GAGCTC	1 cut(s)	1694
Eco57I	CTGAAG	5 cut(s)	1080, 1707, 2471, 2717, 2948
Eco88I	CYCGRG	3 cut(s)	248, 2648, 3115
EcoICRI	GAGCTC	1 cut(s)	1694
EcoNI	CCTNNNNNAGG	1 cut(s)	2900
EcoO109I	RGGNCCY	4 cut(s)	1533, 2192, 2569, 3104
EcoRI	GAATTC	3 cut(s)	35, 1091, 2044
EcoRII	CCWGG	9 cut(s)	40, 830, 1705, 2388, 2804, 2883, 3053, 3145, 3152
EcoRV	GATATC	1 cut(s)	508
EcoT14I	CCWWGG	4 cut(s)	280, 876, 1536, 2076
EcoT38I	GRGCYC	2 cut(s)	267, 1696
ErhI	CCWWGG	4 cut(s)	280, 876, 1536, 2076
Esp3I	CGTCTC	2 cut(s)	787, 2926
FalI	AAGNNNNNCTT	2 cut(s)	2128, 2160
FaqI	GGGAC	2 cut(s)	1489, 1646
FbaI	TGATCA	2 cut(s)	1384, 1860
FblI	GTMKAC	3 cut(s)	432, 1614, 2359
Fnu4HI	GCNGC	8 cut(s)	322, 648, 705, 936, 939, 1299, 1644, 1813
FokI	GGATG	9 cut(s)	302, 319, 587, 682, 847, 1319, 1394, 2009, 2144
FriOI	GRGCYC	2 cut(s)	267, 1696
Fsp4HI	GCNGC	8 cut(s)	322, 648, 705, 936, 939, 1299, 1644, 1813
FspBI	CTAG	6 cut(s)	924, 963, 1329, 1640, 1922, 2318
GlaI	GCGC	2 cut(s)	132, 537
GluI	GCNGC	8 cut(s)	322, 648, 705, 936, 939, 1299, 1644, 1813
GsaI	CCCAGC	1 cut(s)	1272
HaeII	RGCGCY	1 cut(s)	539
HaeIII	GGCC	5 cut(s)	69, 227, 830, 1721, 3105
HapII	CCGG	8 cut(s)	89, 144, 165, 216, 249, 373, 393, 3080
HhaI	GCGC	2 cut(s)	133, 538
Hin6I	GCGC	2 cut(s)	131, 536
HinP1I	GCGC	2 cut(s)	131, 536
HincII	GTYRAC	1 cut(s)	2360
HindII	GTYRAC	1 cut(s)	2360
HindIII	AAGCTT	2 cut(s)	1148, 1886
HpaII	CCGG	8 cut(s)	89, 144, 165, 216, 249, 373, 393, 3080
HphI	GGTGA	4 cut(s)	865, 1404, 1880, 2096
Hpy99I	CGWCG	2 cut(s)	113, 272
HpyCH4III	ACNGT	9 cut(s)	543, 798, 861, 1134, 1282, 1545, 1879, 2349, 3051
HpyCH4IV	ACGT	3 cut(s)	60, 1583, 2533
HpyF10VI	GCNNNNNNNGC	7 cut(s)	139, 192, 533, 1118, 1274, 1833, 2987
HpySE526I	ACGT	3 cut(s)	60, 1583, 2533
HspAI	GCGC	2 cut(s)	131, 536
Ksp22I	TGATCA	2 cut(s)	1384, 1860
LmnI	GCTCC	6 cut(s)	262, 524, 1165, 1340, 1687, 1691
Lsp1109I	GCAGC	6 cut(s)	333, 659, 950, 1310, 1655, 1824
LweI	GCATC	5 cut(s)	671, 1285, 1396, 1845, 2077
MabI	ACCWGGT	1 cut(s)	2388
MaeI	CTAG	6 cut(s)	924, 963, 1329, 1640, 1922, 2318
MaeII	ACGT	3 cut(s)	60, 1583, 2533
MaeIII	GTNAC	7 cut(s)	229, 692, 837, 861, 1126, 2852, 2867
MfeI	CAATTG	2 cut(s)	618, 697
MflI	RGATCY	4 cut(s)	409, 784, 1976, 2684
MhlI	GDGCHC	5 cut(s)	144, 267, 1068, 1696, 2613
MlyI	GAGTC	5 cut(s)	1268, 1880, 2066, 2897, 2906
MmeI	TCCRAC	7 cut(s)	502, 729, 826, 1676, 1765, 2368, 2418
MslI	CAYNNNNRTG	3 cut(s)	875, 1223, 2964
MspA1I	CMGCKG	3 cut(s)	707, 1559, 2632
MspCI	CTTAAG	3 cut(s)	1145, 1151, 1952
MspI	CCGG	8 cut(s)	89, 144, 165, 216, 249, 373, 393, 3080
MunI	CAATTG	2 cut(s)	618, 697
Mva1269I	GAATGC	1 cut(s)	824
MvaI	CCWGG	9 cut(s)	42, 832, 1707, 2390, 2806, 2885, 3055, 3147, 3154
MvnI	CGCG	2 cut(s)	131, 2922
MwoI	GCNNNNNNNGC	7 cut(s)	139, 192, 533, 1118, 1274, 1833, 2987
NciI	CCSGG	5 cut(s)	144, 166, 249, 250, 393
NcoI	CCATGG	1 cut(s)	876
NheI	GCTAGC	2 cut(s)	1639, 1921
NlaIV	GGNNCC	9 cut(s)	264, 800, 1336, 2193, 2779, 2838, 2908, 3144, 3151
NmuCI	GTSAC	2 cut(s)	229, 837
NspI	RCATGY	2 cut(s)	1277, 2747
NspV	TTCGAA	2 cut(s)	1658, 3110
PaeI	GCATGC	1 cut(s)	1277
PaeR7I	CTCGAG	1 cut(s)	2648
PagI	TCATGA	1 cut(s)	2959
PceI	AGGCCT	1 cut(s)	830
PciI	ACATGT	1 cut(s)	2743
PcsI	WCGNNNNNNNCGW	2 cut(s)	99, 443
PctI	GAATGC	1 cut(s)	824
PfeI	GAWTC	7 cut(s)	23, 2314, 2471, 2482, 2540, 2548, 3014
Pfl23II	CGTACG	1 cut(s)	1584
PflMI	CCANNNNNTGG	1 cut(s)	623
PfoI	TCCNGGA	2 cut(s)	391, 3053
PinAI	ACCGGT	2 cut(s)	88, 372
PkrI	GCNGC	8 cut(s)	323, 649, 706, 937, 940, 1300, 1645, 1814
PleI	GAGTC	5 cut(s)	1267, 1879, 2066, 2896, 2906
PpsI	GAGTC	5 cut(s)	1267, 1879, 2066, 2896, 2906
Ppu21I	YACGTR	1 cut(s)	1584
PpuMI	RGGWCCY	3 cut(s)	1533, 2192, 2569
PscI	ACATGT	1 cut(s)	2743
PshBI	ATTAAT	1 cut(s)	2373
PsiI	TTATAA	2 cut(s)	1191, 1443
Psp124BI	GAGCTC	1 cut(s)	1696
Psp1406I	AACGTT	1 cut(s)	60
Psp5II	RGGWCCY	3 cut(s)	1533, 2192, 2569
Psp6I	CCWGG	9 cut(s)	40, 830, 1705, 2388, 2804, 2883, 3053, 3145, 3152
PspFI	CCCAGC	1 cut(s)	1268
PspGI	CCWGG	9 cut(s)	40, 830, 1705, 2388, 2804, 2883, 3053, 3145, 3152
PspLI	CGTACG	1 cut(s)	1584
PspN4I	GGNNCC	9 cut(s)	264, 800, 1336, 2193, 2779, 2838, 2908, 3144, 3151
PspPI	GGNCC	9 cut(s)	68, 157, 443, 990, 1533, 2192, 2569, 2836, 3104
PspPPI	RGGWCCY	3 cut(s)	1533, 2192, 2569
PstNI	CAGNNNCTG	1 cut(s)	1001
PsuI	RGATCY	4 cut(s)	409, 784, 1976, 2684
RsaI	GTAC	3 cut(s)	371, 1586, 2039
RsaNI	GTAC	3 cut(s)	370, 1585, 2038
RseI	CAYNNNNRTG	3 cut(s)	875, 1223, 2964
SacI	GAGCTC	1 cut(s)	1696
SalI	GTCGAC	1 cut(s)	2358
SatI	GCNGC	8 cut(s)	322, 648, 705, 936, 939, 1299, 1644, 1813
Sau96I	GGNCC	9 cut(s)	68, 157, 443, 990, 1533, 2192, 2569, 2836, 3104
SchI	GAGTC	5 cut(s)	1268, 1880, 2066, 2897, 2906
SduI	GDGCHC	5 cut(s)	144, 267, 1068, 1696, 2613
SexAI	ACCWGGT	1 cut(s)	2388
SfaNI	GCATC	5 cut(s)	671, 1285, 1396, 1845, 2077
SfcI	CTRYAG	3 cut(s)	539, 2622, 2863
Sfr274I	CTCGAG	1 cut(s)	2648
SfuI	TTCGAA	2 cut(s)	1658, 3110
SinI	GGWCC	7 cut(s)	157, 443, 990, 1533, 2192, 2569, 2836
SlaI	CTCGAG	1 cut(s)	2648
SmaI	CCCGGG	1 cut(s)	250
SmiMI	CAYNNNNRTG	3 cut(s)	875, 1223, 2964
SmlI	CTYRAG	9 cut(s)	806, 1145, 1151, 1952, 2507, 2573, 2648, 2857, 2900
SmoI	CTYRAG	9 cut(s)	806, 1145, 1151, 1952, 2507, 2573, 2648, 2857, 2900
SnaBI	TACGTA	1 cut(s)	1584
SpeI	ACTAGT	1 cut(s)	1328
SphI	GCATGC	1 cut(s)	1277
SseBI	AGGCCT	1 cut(s)	830
SsiI	CCGC	8 cut(s)	129, 422, 705, 935, 993, 1557, 2632, 2920
SspMI	CTAG	6 cut(s)	924, 963, 1329, 1640, 1922, 2318
SstI	GAGCTC	1 cut(s)	1696
StuI	AGGCCT	1 cut(s)	830
StyI	CCWWGG	4 cut(s)	280, 876, 1536, 2076
TaaI	ACNGT	9 cut(s)	543, 798, 861, 1134, 1282, 1545, 1879, 2349, 3051
TaiI	ACGT	3 cut(s)	63, 1586, 2536
TaqII	GACCGA	1 cut(s)	2420
TauI	GCSGC	2 cut(s)	707, 938
TfiI	GAWTC	7 cut(s)	23, 2314, 2471, 2482, 2540, 2548, 3014
TscAI	CASTG	9 cut(s)	846, 1434, 1777, 1870, 1882, 1918, 3028, 3054, 3094
TseFI	GTSAC	2 cut(s)	229, 837
TseI	GCWGC	6 cut(s)	321, 647, 938, 1298, 1643, 1812
Tsp45I	GTSAC	2 cut(s)	229, 837
TspGWI	ACGGA	5 cut(s)	248, 285, 325, 2025, 2681
TspMI	CCCGGG	1 cut(s)	248
TspRI	CASTG	9 cut(s)	846, 1434, 1777, 1870, 1882, 1918, 3028, 3054, 3094
Van91I	CCANNNNNTGG	1 cut(s)	623
Vha464I	CTTAAG	3 cut(s)	1145, 1151, 1952
VneI	GTGCAC	1 cut(s)	1064
VpaK11BI	GGWCC	7 cut(s)	157, 443, 990, 1533, 2192, 2569, 2836
VspI	ATTAAT	1 cut(s)	2373
XagI	CCTNNNNNAGG	1 cut(s)	2900
XapI	RAATTY	9 cut(s)	35, 717, 752, 1070, 1091, 1289, 1575, 1855, 2044
XbaI	TCTAGA	1 cut(s)	2317
XceI	RCATGY	2 cut(s)	1277, 2747
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2830
XhoI	CTCGAG	1 cut(s)	2648
XmaI	CCCGGG	1 cut(s)	248
XmiI	GTMKAC	3 cut(s)	432, 1614, 2359
XspI	CTAG	6 cut(s)	924, 963, 1329, 1640, 1922, 2318

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.