Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002382

Physical Location & Seq

Genomic Coordinates Reverse (-)

48131487 .. 48137736

Gene Structure 6250 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1388700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3186 bp

ATGGCAGAGGCACAGCCCACCGATTCCCACGCTGAATTCCCTGGTTCTTGGTCTTGCAACGTTCTTGGCCTCGAAACACACCCCAAACCGATCGACAGTGGCCATGTCGCTGACGACGAAGACTCGACCCTCTCCGCGCTCTGTGTGCGAGGTTCGAGATTGGTCCGAACCGGGTTTGTTCAGTCCACTTGCTCAGATAGCATTGTGTTATTTGTGAATGCCGGAGGGGAGGCTGTAAGGGAAAACATAAATCCCGGGATTCGAGTTAAGCCTGACGGGTTTTTTCAAGGAGGTGATGTTCTAAGAACTAACGAGACCATTGACGGCAGTGATGTGCCATCTGTTTATCAATCTGCAAGGTTTGGGGATTTTTCGTACCGTTTTGAGAACCTGTCTCCAGGAGATTACTTCGTGGATCTTCACTTTGCGGAAATTGTATACACGAATGGTCCGAAAGGAATGAGAGTGTTTGATGTGTTCATGCAGGACGAGAAGGTTCTATCAGGAATTGATATCTATTCTATCGTTGGAGCTAATAAGGCGCTACAGTTGGTAGATGTTGCAGTTTCGGTGGGGGAGGATGGGGCAATTTTGATAAGGTTTGATGGAGTTAATGGAAGTCCAATTGTTGGGGGAATTTGTATAAAGCAGGAAGCTACGTTACCTGCATCAAAGATGAAACTTGGACATCTTGTATGTAAAAATTGTGCTGCTGAGATAGAAATTTCATCTGCTCAGGACAAATATATGAGGTTGGAATTTACAACCAAGTATGAAAAGAAGATAGAGGATCTGAAGAGACAGTTCCAGCTCAAGACAGATGAATGCCATGAGGCCTGGATGTCACTGACATCTGCAAACAAACAGTTACAGAATATCACCATGGAACTCGATAACAAGTTGTATGAAAGTCATTGCTTAGATCATGCTAGAGACGAAGCTGAAGCAAAATTGAGAGATATTTCTACTAGATATGAGCATGATAAGGGGTTGTGGTCCGCAGTTGCTGACAATTTAGAAAGTAAAGTCAAGTTGATGAAGGAAGACTATTCTCAACTCTATTCTGAAGTGCACAAATTTACTGATGTAATTCCAGAATTCAACAATATGGTACTTGCAATTCAAACGATGGTTGAACAGTCTGATGAACTTAAGCTTAAGTTCAATGAGGAGCAGGCAAAGAGGAAGGTACTTTATAACCAAATGCAGGAGGCAAAAGGGAACATTCGGGTGTTTTGTCGGTGTCGCCCATTGCGGAAGGATGAGTTATTAGCTGGGCATGCAACAGTTGTAAAATTTGACGCAGCTAAGGATGGAGAACTTGGGATACTTACTAGTGGGTCCACTAAAAAGATGTTCAGATTTGACAGAGTTTACACACCAGAGGATGATCAAGTTGATGTATTTGCAGATGCTTCACCAATGATAACCTCAGTGTTAGATGGTTATAATGTTTGCATATTTGCCTATGGGCAAACGGGGACTGGAAAAACTTTCACGATGGAAGGTTCTGAAACTAATCGAGGGGTCAATTACAGGACCTTGGAACAGTTGTTTAAAACCGCTGAGGAAAGGAAAGAAATTTTTACATATAGCATATCTGTTAGCGTGCTTGAAGTTTATAATGAGCAAATCAGAGACTTGCTAGCAGCCTCTCCGACATCGAAAAGGTTGGAGATGAGGCAAGCTCCTGAAGGTGTTCATCACATACCTGGAATTGTTGAAGCCAAAGTTGAGAGCATTAAAGAGGTTTGGAATGTTCTGCAAGCTGGAAGCAGTGCAAGGGCAGTTGGATCAAACAATGTGAACGAGCATAGCAGCCGATCTCATTGCATACTCTGCATCATGGTAAAAGCTCAGAATTTGATCACTGGTGAGTCCACAATGAGCAAGCTTTGGCTTGTGGATTTGGCTGGCAGTGAGAGACTAGCAAAAACTGAAGTTCAAGGGGAACGCCTTAAGGAAGCTCAAAACATCAATAGATCTCTTTCAGCTCTGGGGGATGTCATCTCAGCTTTGGCAACAAAAAGAAGCCACATTCCATACAGGAATTCCAAGCTCACGCATCTACTTCAAGACTCCTTGGGTGGTGATGCTAAAACTCTAATGTTTGTCCAAATAAGCCCGTCTGAGCAGGATGTAAGTGAGACTCTAAGTTCTCTTAACTTTGCAACACGGGTTCGAGGTGTTGAATTGGGTCCTGCAAGGAAGCAAATAGATACGGGTGAGGTTCAAAAGTTAAAACTAATGCTTGACAAGCTAAAGCAAGAGTTGAAATCCAAAGATGATGATTTACATAAATTAGAAGAGAACTTGCAGAATCGAGAAGGTAAAGCAAAACGGGAGTTTCAACTGTCTAGAAGTCGACAAGAAGAGATTAATGAACTTGAAGGCCAGATTGCTGTGAAGACCGATGTGTGCAGACAACTGGAAAATCAGTTGTTGGAACTTTCAGATGAAGCAAATGGAAAGAAAGAATCATGCTTGAATCTTCACAAAAAGGTCAAAGAGCTTGAGGACAAGTTGAAAGAACGGGAACATGCAGATTCCATGGTTCTTCAAAACAAGGTCAGGGACCTCGAGAACAAGTTAAAAGAAAGAACGCAAGAGTATGAGCACAATACTGCCGTACTTCAGTGGAAGGTTCAAGAACTCGAGAAAAGGCTGATAACGGAGGAAAACAACAAGGGATCTCTGCAACTGAAGGTTAAAGAGCTTGAAGAGAAATTGAGACAGAAAGAACAAGAACATGTGATATCGACATTTTGTGGCAACAAGTCAGCTGCATCAACCCCCAATGACAGAAAATCCTGGCCGAGAAGAGAGACTATTGGTGGTGACATAGACCCGTTGAGGCTAAGTAACTTGAGCGATAGTAACCAAATGACAATCCGGGAGTCCATCCTACTCAAGGGAACCGACTCCCTTCGCGAGTTGAGGAGACGAAGAGAGTTGCACAGCAAAGGTCATGAGAATGTGATGTTGCCAGCTACTTTGGCTGATAGGAAGATGTCATCAGCCGAATCCAGCACTGACAAGTCTGGTGACCAAAAACCACTGTCCAGGATCACAAGAAGTTTAACAAGACCGGCAGCCACTGGTCAAAAACCAGGCCCTTCGAACCCGAGCAACAAAAACTATAGAGATCAGGTTCCAGGTTCCAGGTCAAGGACTTGGTTACGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin Malectin

Protein Analysis

1062

Amino Acids

119.35

Weight (kDa)

6.2

Isoelectric Point (pI)

42.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin	PF11721	69 - 193	4.4e-18	Malectin domain
Microtub_bd	PF16796	389 - 549	4.3e-44	Microtubule binding
Kinesin	PF00225	414 - 729	1.6e-115	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22610 AT2G22610 AT2G22610
fragaria_vesca	FvH4_1g19311 FvH4_1g19311 FvH4_1g19311 FvH4_1g19311 FvH4_1g19311 FvH4_5g34300 FvH4_5g34300
malus_domestica	MD08G1202900.v1.1 MD15G1388700.v1.1
prunus_persica	Prupe.1G535100_v2.0.a1 Prupe.6G205100_v2.0.a1 Prupe.6G205100_v2.0.a1 Prupe.6G205100_v2.0.a1 Prupe.6G205100_v2.0.a1 Prupe.6G205100_v2.0.a1 Prupe.6G205100_v2.0.a1
pyrus_communis	pycom08g17520 pycom15g34860
rosa_chinensis	RchiOBHm_Chr2g0110681 RchiOBHm_Chr7g0234871
rosa_laevigata	RLG00000001208 RLG00000017830
rosa_multiflora	Rmu_sc0000039.1_g000002 Rmu_sc0000389.1_g000040 Rmu_sc0040758.1_g000001
rosa_roxburghii	Rroxscaffold_2G00133040 Rroxscaffold_3G00226690
rosa_rugosa	Rorug02G0168600 Rorug02G0168600 Rorug07G0285900
rosa_samantha	Rh2AG220900 Rh2BG231000 Rh2CG223300 Rh2DG227100 Rh7AG440500 Rh7BG413300 Rh7CG462200 Rh7DG430200
rosa_wichuraiana	Rw7G036620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1197, 1449, 1623
Acc36I	ACCTGC	1 cut(s)	673
AccB7I	CCANNNNNTGG	1 cut(s)	629
AccI	GTMKAC	2 cut(s)	438, 2365
AccII	CGCG	2 cut(s)	137, 2931
AciI	CCGC	5 cut(s)	135, 428, 999, 1255, 1563
AclI	AACGTT	1 cut(s)	60
AclWI	GGATC	5 cut(s)	423, 798, 1801, 2698, 3074
AcoI	YGGCCR	2 cut(s)	100, 2813
AcuI	CTGAAG	7 cut(s)	815, 963, 1086, 1713, 1959, 2618, 2723
AfaI	GTAC	4 cut(s)	377, 1113, 1191, 2631
AfiI	CCNNNNNNNGG	4 cut(s)	629, 1207, 2911, 3168
AflII	CTTAAG	3 cut(s)	1151, 1157, 1958
AflIII	ACRYGT	1 cut(s)	2749
AhlI	ACTAGT	1 cut(s)	1334
AjnI	CCWGG	9 cut(s)	40, 397, 836, 1711, 2810, 3062, 3109, 3154, 3161
AloI	GAACNNNNNNTCC	2 cut(s)	282, 314
Alw21I	GWGCWC	2 cut(s)	1074, 2619
Alw44I	GTGCAC	1 cut(s)	1070
AlwI	GGATC	5 cut(s)	423, 798, 1801, 2698, 3074
AlwNI	CAGNNNCTG	2 cut(s)	1007, 3098
Ama87I	CYCGRG	4 cut(s)	254, 2579, 2654, 3124
AoxI	GGCC	6 cut(s)	67, 100, 834, 2392, 2813, 3112
ApaLI	GTGCAC	1 cut(s)	1070
ApeKI	GCWGC	6 cut(s)	710, 1304, 1649, 1818, 2783, 3092
AseI	ATTAAT	1 cut(s)	2379
Asp700I	GAANNNNTTC	1 cut(s)	1698
AspLEI	GCGC	2 cut(s)	139, 544
AspS9I	GGNCC	8 cut(s)	163, 449, 996, 1341, 1539, 2198, 2575, 3113
AsuC2I	CCSGG	4 cut(s)	172, 255, 256, 2894
AsuHPI	GGTGA	8 cut(s)	305, 871, 1410, 1886, 2102, 2237, 2849, 3056
AsuII	TTCGAA	1 cut(s)	3119
AsuNHI	GCTAGC	1 cut(s)	1645
AvaI	CYCGRG	4 cut(s)	254, 2579, 2654, 3124
AvaII	GGWCC	7 cut(s)	163, 449, 996, 1341, 1539, 2198, 2575
BaeGI	GKGCMC	1 cut(s)	1074
BalI	TGGCCA	1 cut(s)	102
BarI	GAAGNNNNNNTAC	2 cut(s)	645, 677
BbsI	GAAGAC	3 cut(s)	126, 1050, 2414
Bbv12I	GWGCWC	2 cut(s)	1074, 2619
BbvCI	CCTCAGC	1 cut(s)	1566
BbvI	GCAGC	6 cut(s)	697, 1316, 1661, 1830, 2770, 3104
BccI	CCATC	8 cut(s)	346, 575, 599, 1123, 1307, 1436, 1495, 2909
BceAI	ACGGC	2 cut(s)	340, 2612
BcgI	CGANNNNNNTGC	2 cut(s)	1812, 1846
BciT130I	CCWGG	9 cut(s)	42, 399, 838, 1713, 2812, 3064, 3111, 3156, 3163
BciVI	GTATCC	1 cut(s)	1320
BclI	TGATCA	2 cut(s)	1390, 1866
BcnI	CCSGG	4 cut(s)	172, 255, 256, 2894
BcuI	ACTAGT	1 cut(s)	1334
BfaI	CTAG	6 cut(s)	930, 969, 1335, 1646, 1928, 2358
BfmI	CTRYAG	2 cut(s)	545, 3139
BfoI	RGCGCY	1 cut(s)	545
BfrI	CTTAAG	3 cut(s)	1151, 1157, 1958
BfuAI	ACCTGC	1 cut(s)	673
BfuI	GTATCC	1 cut(s)	1320
BglII	AGATCT	1 cut(s)	1982
BisI	GCNGC	6 cut(s)	711, 1305, 1650, 1819, 2784, 3093
BlsI	GCNGC	6 cut(s)	712, 1306, 1651, 1820, 2785, 3094
Bme18I	GGWCC	7 cut(s)	163, 449, 996, 1341, 1539, 2198, 2575
BmeT110I	CYCGRG	4 cut(s)	254, 2579, 2654, 3124
BmgT120I	GGNCC	8 cut(s)	163, 449, 996, 1341, 1539, 2198, 2575, 3113
BmiI	GGNNCC	6 cut(s)	1342, 2199, 2576, 2917, 3153, 3160
BmsI	GCATC	6 cut(s)	677, 1402, 1851, 2074, 2083, 2795
BmtI	GCTAGC	1 cut(s)	1649
BpiI	GAAGAC	3 cut(s)	126, 1050, 2414
BplI	GAGNNNNNCTC	2 cut(s)	1672, 1704
BpmI	CTGGAG	1 cut(s)	381
Bpu10I	CCTNAGC	3 cut(s)	735, 1308, 1566
Bpu14I	TTCGAA	1 cut(s)	3119
BpuEI	CTTGAG	4 cut(s)	797, 2534, 2887, 2894
BpuMI	CCSGG	4 cut(s)	172, 255, 256, 2894
BsaI	GGTCTC	1 cut(s)	308
BsaJI	CCNNGG	6 cut(s)	40, 254, 882, 1542, 2082, 2550
BsaXI	ACNNNNNCTCC	2 cut(s)	282, 312
Bsc4I	CCNNNNNNNGG	4 cut(s)	629, 1207, 2911, 3168
Bse118I	RCCGGY	1 cut(s)	3088
Bse1I	ACTGG	4 cut(s)	1489, 1876, 2433, 3103
Bse3DI	GCAATG	3 cut(s)	913, 1250, 1828
BseBI	CCWGG	9 cut(s)	42, 399, 838, 1713, 2812, 3064, 3111, 3156, 3163
BseDI	CCNNGG	6 cut(s)	40, 254, 882, 1542, 2082, 2550
BseGI	GGATG	8 cut(s)	586, 846, 1267, 1318, 1393, 2008, 2143, 2901
BseLI	CCNNNNNNNGG	4 cut(s)	629, 1207, 2911, 3168
BseMI	GCAATG	3 cut(s)	913, 1250, 1828
BseMII	CTCAG	8 cut(s)	207, 705, 749, 1446, 1557, 1871, 2025, 2121
BseNI	ACTGG	4 cut(s)	1489, 1876, 2433, 3103
BseRI	GAGGAG	2 cut(s)	1184, 2953
BseSI	GKGCMC	1 cut(s)	1074
BseXI	GCAGC	6 cut(s)	697, 1316, 1661, 1830, 2770, 3104
BseYI	CCCAGC	1 cut(s)	1274
BsgI	GTGCAG	1 cut(s)	2440
Bsh1236I	CGCG	2 cut(s)	137, 2931
Bsh1285I	CGRYCG	1 cut(s)	93
BshFI	GGCC	6 cut(s)	69, 102, 836, 2394, 2815, 3114
BsiEI	CGRYCG	1 cut(s)	93
BsiHKAI	GWGCWC	2 cut(s)	1074, 2619
BsiHKCI	CYCGRG	4 cut(s)	254, 2579, 2654, 3124
BsiSI	CCGG	5 cut(s)	171, 222, 255, 2893, 3089
BslFI	GGGAC	2 cut(s)	1495, 2588
BslI	CCNNNNNNNGG	4 cut(s)	629, 1207, 2911, 3168
BsmBI	CGTCTC	2 cut(s)	927, 2935
BsmFI	GGGAC	2 cut(s)	1495, 2588
BsmI	GAATGC	2 cut(s)	223, 830
BsnI	GGCC	6 cut(s)	69, 102, 836, 2394, 2815, 3114
Bso31I	GGTCTC	1 cut(s)	308
BsoBI	CYCGRG	4 cut(s)	254, 2579, 2654, 3124
Bsp119I	TTCGAA	1 cut(s)	3119
Bsp1286I	GDGCHC	2 cut(s)	1074, 2619
Bsp19I	CCATGG	2 cut(s)	882, 2550
Bsp68I	TCGCGA	1 cut(s)	2931
BspACI	CCGC	5 cut(s)	135, 428, 999, 1255, 1563
BspANI	GGCC	6 cut(s)	69, 102, 836, 2394, 2815, 3114
BspCNI	CTCAG	8 cut(s)	206, 706, 748, 1445, 1558, 1870, 2024, 2122
BspFNI	CGCG	2 cut(s)	137, 2931
BspHI	TCATGA	1 cut(s)	2968
BspLI	GGNNCC	6 cut(s)	1342, 2199, 2576, 2917, 3153, 3160
BspMI	ACCTGC	1 cut(s)	673
BspOI	GCTAGC	1 cut(s)	1649
BspPI	GGATC	5 cut(s)	423, 798, 1801, 2698, 3074
BspT104I	TTCGAA	1 cut(s)	3119
BspTI	CTTAAG	3 cut(s)	1151, 1157, 1958
BspTNI	GGTCTC	1 cut(s)	308
BsrDI	GCAATG	3 cut(s)	913, 1250, 1828
BsrFI	RCCGGY	1 cut(s)	3088
BsrI	ACTGG	4 cut(s)	1489, 1876, 2433, 3103
BssAI	RCCGGY	1 cut(s)	3088
BssECI	CCNNGG	6 cut(s)	40, 254, 882, 1542, 2082, 2550
BssNAI	GTATAC	1 cut(s)	439
BssT1I	CCWWGG	4 cut(s)	882, 1542, 2082, 2550
Bst1107I	GTATAC	1 cut(s)	439
Bst2UI	CCWGG	9 cut(s)	42, 399, 838, 1713, 2812, 3064, 3111, 3156, 3163
Bst6I	CTCTTC	6 cut(s)	791, 2301, 2367, 2715, 2815, 2941
BstAFI	CTTAAG	3 cut(s)	1151, 1157, 1958
BstAPI	GCANNNNNTGC	1 cut(s)	1839
BstBI	TTCGAA	1 cut(s)	3119
BstC8I	GCNNGC	9 cut(s)	1176, 1281, 1610, 1647, 1686, 1767, 1892, 1915, 2988
BstDSI	CCRYGG	2 cut(s)	882, 2550
BstEII	GGTNACC	1 cut(s)	3044
BstENI	CCTNNNNNAGG	1 cut(s)	2909
BstF5I	GGATG	8 cut(s)	586, 846, 1267, 1318, 1393, 2008, 2143, 2901
BstFNI	CGCG	2 cut(s)	137, 2931
BstH2I	RGCGCY	1 cut(s)	545
BstHHI	GCGC	2 cut(s)	139, 544
BstMCI	CGRYCG	1 cut(s)	93
BstMWI	GCNNNNNNNGC	7 cut(s)	145, 198, 539, 1280, 1839, 2257, 2996
BstNI	CCWGG	9 cut(s)	42, 399, 838, 1713, 2812, 3064, 3111, 3156, 3163
BstNSI	RCATGY	3 cut(s)	1283, 2543, 2753
BstPI	GGTNACC	1 cut(s)	3044
BstSFI	CTRYAG	2 cut(s)	545, 3139
BstSLI	GKGCMC	1 cut(s)	1074
BstUI	CGCG	2 cut(s)	137, 2931
BstV1I	GCAGC	6 cut(s)	697, 1316, 1661, 1830, 2770, 3104
BstV2I	GAAGAC	3 cut(s)	126, 1050, 2414
BstX2I	RGATCY	4 cut(s)	415, 790, 1982, 2690
BstYI	RGATCY	4 cut(s)	415, 790, 1982, 2690
BstZ17I	GTATAC	1 cut(s)	439
BsuI	GTATCC	1 cut(s)	1320
BsuRI	GGCC	6 cut(s)	69, 102, 836, 2394, 2815, 3114
BtgI	CCRYGG	2 cut(s)	882, 2550
BtsCI	GGATG	8 cut(s)	586, 846, 1267, 1318, 1393, 2008, 2143, 2901
BtsI	GCAGTG	3 cut(s)	334, 1783, 1924
BtuMI	TCGCGA	1 cut(s)	2931
BveI	ACCTGC	1 cut(s)	673
Cac8I	GCNNGC	9 cut(s)	1176, 1281, 1610, 1647, 1686, 1767, 1892, 1915, 2988
CaiI	CAGNNNCTG	2 cut(s)	1007, 3098
CciI	TCATGA	1 cut(s)	2968
CfoI	GCGC	2 cut(s)	139, 544
Cfr10I	RCCGGY	1 cut(s)	3088
Cfr13I	GGNCC	8 cut(s)	163, 449, 996, 1341, 1539, 2198, 2575, 3113
Cfr9I	CCCGGG	1 cut(s)	254
CseI	GACGC	1 cut(s)	1310
Csp6I	GTAC	4 cut(s)	376, 1112, 1190, 2630
CviQI	GTAC	4 cut(s)	376, 1112, 1190, 2630
DraI	TTTAAA	1 cut(s)	1558
EaeI	YGGCCR	2 cut(s)	100, 2813
Eam1104I	CTCTTC	6 cut(s)	791, 2301, 2367, 2715, 2815, 2941
EarI	CTCTTC	6 cut(s)	791, 2301, 2367, 2715, 2815, 2941
Eco130I	CCWWGG	4 cut(s)	882, 1542, 2082, 2550
Eco147I	AGGCCT	1 cut(s)	836
Eco31I	GGTCTC	1 cut(s)	308
Eco32I	GATATC	2 cut(s)	514, 2757
Eco47I	GGWCC	7 cut(s)	163, 449, 996, 1341, 1539, 2198, 2575
Eco57I	CTGAAG	7 cut(s)	815, 963, 1086, 1713, 1959, 2618, 2723
Eco88I	CYCGRG	4 cut(s)	254, 2579, 2654, 3124
Eco91I	GGTNACC	1 cut(s)	3044
EcoNI	CCTNNNNNAGG	1 cut(s)	2909
EcoO109I	RGGNCCY	4 cut(s)	1539, 2198, 2575, 3113
EcoO65I	GGTNACC	1 cut(s)	3044
EcoRI	GAATTC	3 cut(s)	35, 1097, 2050
EcoRII	CCWGG	9 cut(s)	40, 397, 836, 1711, 2810, 3062, 3109, 3154, 3161
EcoRV	GATATC	2 cut(s)	514, 2757
EcoT14I	CCWWGG	4 cut(s)	882, 1542, 2082, 2550
ErhI	CCWWGG	4 cut(s)	882, 1542, 2082, 2550
Esp3I	CGTCTC	2 cut(s)	927, 2935
FaqI	GGGAC	2 cut(s)	1495, 2588
FbaI	TGATCA	2 cut(s)	1390, 1866
FblI	GTMKAC	2 cut(s)	438, 2365
Fnu4HI	GCNGC	6 cut(s)	711, 1305, 1650, 1819, 2784, 3093
FokI	GGATG	8 cut(s)	593, 853, 1274, 1325, 1400, 2015, 2150, 2888
Fsp4HI	GCNGC	6 cut(s)	711, 1305, 1650, 1819, 2784, 3093
FspBI	CTAG	6 cut(s)	930, 969, 1335, 1646, 1928, 2358
GlaI	GCGC	2 cut(s)	138, 543
GluI	GCNGC	6 cut(s)	711, 1305, 1650, 1819, 2784, 3093
GsaI	CCCAGC	1 cut(s)	1278
GsuI	CTGGAG	1 cut(s)	381
HaeII	RGCGCY	1 cut(s)	545
HaeIII	GGCC	6 cut(s)	69, 102, 836, 2394, 2815, 3114
HapII	CCGG	5 cut(s)	171, 222, 255, 2893, 3089
HgaI	GACGC	1 cut(s)	1310
HhaI	GCGC	2 cut(s)	139, 544
Hin6I	GCGC	2 cut(s)	137, 542
HinP1I	GCGC	2 cut(s)	137, 542
HincII	GTYRAC	1 cut(s)	2366
HindII	GTYRAC	1 cut(s)	2366
HindIII	AAGCTT	2 cut(s)	1154, 1892
HpaII	CCGG	5 cut(s)	171, 222, 255, 2893, 3089
HphI	GGTGA	8 cut(s)	305, 871, 1410, 1886, 2102, 2237, 2849, 3056
Hpy166II	GTNNAC	8 cut(s)	186, 439, 1072, 1344, 1375, 1807, 1881, 2366
Hpy8I	GTNNAC	8 cut(s)	186, 439, 1072, 1344, 1375, 1807, 1881, 2366
Hpy99I	CGWCG	1 cut(s)	119
HpyCH4IV	ACGT	2 cut(s)	60, 659
HpyF10VI	GCNNNNNNNGC	7 cut(s)	145, 198, 539, 1280, 1839, 2257, 2996
HpySE526I	ACGT	2 cut(s)	60, 659
HspAI	GCGC	2 cut(s)	137, 542
Ksp22I	TGATCA	2 cut(s)	1390, 1866
LmnI	GCTCC	3 cut(s)	530, 1171, 1693
Lsp1109I	GCAGC	6 cut(s)	697, 1316, 1661, 1830, 2770, 3104
LweI	GCATC	6 cut(s)	677, 1402, 1851, 2074, 2083, 2795
MaeI	CTAG	6 cut(s)	930, 969, 1335, 1646, 1928, 2358
MaeII	ACGT	2 cut(s)	60, 659
MaeIII	GTNAC	8 cut(s)	660, 843, 867, 2837, 2861, 2876, 3044, 3177
MfeI	CAATTG	1 cut(s)	624
MflI	RGATCY	4 cut(s)	415, 790, 1982, 2690
MhlI	GDGCHC	2 cut(s)	1074, 2619
MlsI	TGGCCA	1 cut(s)	102
MluNI	TGGCCA	1 cut(s)	102
MlyI	GAGTC	6 cut(s)	116, 1886, 2072, 2143, 2906, 2915
MmeI	TCCRAC	6 cut(s)	508, 735, 1653, 1682, 1771, 2424
Mox20I	TGGCCA	1 cut(s)	102
MroXI	GAANNNNTTC	1 cut(s)	1698
MscI	TGGCCA	1 cut(s)	102
MslI	CAYNNNNRTG	2 cut(s)	1229, 2973
Msp20I	TGGCCA	1 cut(s)	102
MspA1I	CMGCKG	2 cut(s)	1565, 2783
MspCI	CTTAAG	3 cut(s)	1151, 1157, 1958
MspI	CCGG	5 cut(s)	171, 222, 255, 2893, 3089
MunI	CAATTG	1 cut(s)	624
Mva1269I	GAATGC	2 cut(s)	223, 830
MvaI	CCWGG	9 cut(s)	42, 399, 838, 1713, 2812, 3064, 3111, 3156, 3163
MvnI	CGCG	2 cut(s)	137, 2931
MwoI	GCNNNNNNNGC	7 cut(s)	145, 198, 539, 1280, 1839, 2257, 2996
NciI	CCSGG	4 cut(s)	172, 255, 256, 2894
NcoI	CCATGG	2 cut(s)	882, 2550
NheI	GCTAGC	1 cut(s)	1645
NlaIV	GGNNCC	6 cut(s)	1342, 2199, 2576, 2917, 3153, 3160
NmeAIII	GCCGAG	1 cut(s)	2841
NmuCI	GTSAC	3 cut(s)	843, 2837, 3044
NruI	TCGCGA	1 cut(s)	2931
NspI	RCATGY	3 cut(s)	1283, 2543, 2753
NspV	TTCGAA	1 cut(s)	3119
PaeI	GCATGC	1 cut(s)	1283
PaeR7I	CTCGAG	2 cut(s)	2579, 2654
PagI	TCATGA	1 cut(s)	2968
PceI	AGGCCT	1 cut(s)	836
PciI	ACATGT	1 cut(s)	2749
PcsI	WCGNNNNNNNCGW	2 cut(s)	114, 449
PctI	GAATGC	2 cut(s)	223, 830
PdmI	GAANNNNTTC	1 cut(s)	1698
PfeI	GAWTC	7 cut(s)	23, 259, 2320, 2477, 2488, 2546, 3023
PflMI	CCANNNNNTGG	1 cut(s)	629
PfoI	TCCNGGA	3 cut(s)	397, 2892, 3062
PkrI	GCNGC	6 cut(s)	712, 1306, 1651, 1820, 2785, 3094
Ple19I	CGATCG	1 cut(s)	93
PleI	GAGTC	6 cut(s)	116, 1885, 2072, 2143, 2905, 2915
PpsI	GAGTC	6 cut(s)	116, 1885, 2072, 2143, 2905, 2915
PpuMI	RGGWCCY	3 cut(s)	1539, 2198, 2575
PscI	ACATGT	1 cut(s)	2749
PshBI	ATTAAT	1 cut(s)	2379
PsiI	TTATAA	3 cut(s)	1197, 1449, 1623
Psp1406I	AACGTT	1 cut(s)	60
Psp5II	RGGWCCY	3 cut(s)	1539, 2198, 2575
Psp6I	CCWGG	9 cut(s)	40, 397, 836, 1711, 2810, 3062, 3109, 3154, 3161
PspEI	GGTNACC	1 cut(s)	3044
PspFI	CCCAGC	1 cut(s)	1274
PspGI	CCWGG	9 cut(s)	40, 397, 836, 1711, 2810, 3062, 3109, 3154, 3161
PspN4I	GGNNCC	6 cut(s)	1342, 2199, 2576, 2917, 3153, 3160
PspPI	GGNCC	8 cut(s)	163, 449, 996, 1341, 1539, 2198, 2575, 3113
PspPPI	RGGWCCY	3 cut(s)	1539, 2198, 2575
PsrI	GAACNNNNNNTAC	2 cut(s)	1311, 1343
PstNI	CAGNNNCTG	2 cut(s)	1007, 3098
PsuI	RGATCY	4 cut(s)	415, 790, 1982, 2690
PvuI	CGATCG	1 cut(s)	93
PvuII	CAGCTG	1 cut(s)	2783
RruI	TCGCGA	1 cut(s)	2931
RsaI	GTAC	4 cut(s)	377, 1113, 1191, 2631
RsaNI	GTAC	4 cut(s)	376, 1112, 1190, 2630
RseI	CAYNNNNRTG	2 cut(s)	1229, 2973
SalI	GTCGAC	1 cut(s)	2364
SatI	GCNGC	6 cut(s)	711, 1305, 1650, 1819, 2784, 3093
Sau96I	GGNCC	8 cut(s)	163, 449, 996, 1341, 1539, 2198, 2575, 3113
SchI	GAGTC	6 cut(s)	116, 1886, 2072, 2143, 2906, 2915
SduI	GDGCHC	2 cut(s)	1074, 2619
SfaNI	GCATC	6 cut(s)	677, 1402, 1851, 2074, 2083, 2795
SfcI	CTRYAG	2 cut(s)	545, 3139
Sfr274I	CTCGAG	2 cut(s)	2579, 2654
SfuI	TTCGAA	1 cut(s)	3119
SinI	GGWCC	7 cut(s)	163, 449, 996, 1341, 1539, 2198, 2575
SlaI	CTCGAG	2 cut(s)	2579, 2654
SmaI	CCCGGG	1 cut(s)	256
SmiMI	CAYNNNNRTG	2 cut(s)	1229, 2973
SmlI	CTYRAG	9 cut(s)	812, 1151, 1157, 1958, 2513, 2579, 2654, 2866, 2909
SmoI	CTYRAG	9 cut(s)	812, 1151, 1157, 1958, 2513, 2579, 2654, 2866, 2909
SpeI	ACTAGT	1 cut(s)	1334
SphI	GCATGC	1 cut(s)	1283
SseBI	AGGCCT	1 cut(s)	836
SsiI	CCGC	5 cut(s)	135, 428, 999, 1255, 1563
SspMI	CTAG	6 cut(s)	930, 969, 1335, 1646, 1928, 2358
StuI	AGGCCT	1 cut(s)	836
StyI	CCWWGG	4 cut(s)	882, 1542, 2082, 2550
TaiI	ACGT	2 cut(s)	63, 662
TaqII	GACCGA	1 cut(s)	2426
TfiI	GAWTC	7 cut(s)	23, 259, 2320, 2477, 2488, 2546, 3023
TseFI	GTSAC	3 cut(s)	843, 2837, 3044
TseI	GCWGC	6 cut(s)	710, 1304, 1649, 1818, 2783, 3092
Tsp45I	GTSAC	3 cut(s)	843, 2837, 3044
TspGWI	ACGGA	1 cut(s)	2687
TspMI	CCCGGG	1 cut(s)	254
Van91I	CCANNNNNTGG	1 cut(s)	629
Vha464I	CTTAAG	3 cut(s)	1151, 1157, 1958
VneI	GTGCAC	1 cut(s)	1070
VpaK11BI	GGWCC	7 cut(s)	163, 449, 996, 1341, 1539, 2198, 2575
VspI	ATTAAT	1 cut(s)	2379
XagI	CCTNNNNNAGG	1 cut(s)	2909
XbaI	TCTAGA	1 cut(s)	2357
XceI	RCATGY	3 cut(s)	1283, 2543, 2753
XhoI	CTCGAG	2 cut(s)	2579, 2654
XmaI	CCCGGG	1 cut(s)	254
XmiI	GTMKAC	2 cut(s)	438, 2365
XmnI	GAANNNNTTC	1 cut(s)	1698
XspI	CTAG	6 cut(s)	930, 969, 1335, 1646, 1928, 2358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.