Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0015327

Physical Location & Seq

Genomic Coordinates Reverse (-)

27289656 .. 27293414

Gene Structure 3759 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1209800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1695 bp

ATGTGCAACCCAAAGGCTTCTTCTTCCTCCCCACTTCTCTCTCCCAACAAAACCAATTTCATGGAAACCTCCAACAAAACCATCGACGACCTTCACTGTTCTGATGTTTTTGTTGAAGACGACGATAAAGAGCTCCATAGATGGCCTACTCTCTCAGAGGCTGTACAAGAAATCAAGGCAATAGGGAAGATCTCAGGTCCATCAACCATGACAGGCCTACTTCTCTACTCAAGAGCCATGATCTCCATGCTTTTTCTAGGATATCTCGGTGAGCTTGAGCTCGCCGGCGGCTCTCTCTCAATTGGATTTGCCAACATCACTGGCTACTCTGTCATCTCCGGCCTCGCCATGGGAATGGAACCCATCTGCGGGCAAGCTTACGGGGCCAAACAGATGAAGCTCCTCGGCCTAACCCTTCAGAGAACCGTCCTCCTTCTCCTCTCTACCTCCCTCCCCATCTCCTTCATGTGGTTCAACATGAAAAGAGTCCTCCTCTGGTTTGGCCAGGACGAAGAGATTTCCTCCGTCGCCCACACTTTCATCCTCTTCTCCACTCCTGACCTCTTCTTTCTCTCTCTTCTCCACCCCCTCAGAATTTATCTCCGCACCCAAAACATCACATTGCCAGTGACCTACTGCTCCGCCATCTCTGTCCTCCTCCACATCCCTCTAAACTTCCTCCTAGTCGTTAAATTCCAAATGGGGATTTCCGGAGTGGCCATAGCCATGGTTTGGACTAATCTCAACCTCTTCATCCTGCTCTTCTCCTTCACGTACTTCTCCAATGTTTATAAGGACTCGTGGGTTTGTCCCAGCGCTGATTGCCTCCGCGGCTGGTCATCTTTGCTTGCGCTCGCTATCCCGACCTGCATCTCCGTTTGCCTCGAATGGTGGTGGTATGAGTTCATGATAATGCTGTGCGGACTGTTAGCCAACCCGAAAGCCACCATTTCTTCAATGGGAATACTTATCCAGACCACCTCTCTTGTCTACGTGTTCCCTTCTTCGCTTAGCCTCGCCGTCTCCACGAGAGTTGGGAATGAGTTGGGCGCTAATCGACCGTCACGAGCACGCATTTCCATGATCGTCTCGCTCGGTTGTTCAGTGGCTTTAGGCCTTTTAGCCATGCTGTTCACAACATTTATGAGGCACCAATGGGGGAGATTCTTCACTAATGACGCCGAGATACTCAAGCTCACGGCGATTGCATTGCCGATAGCAGGGCTGTGCGAACTCGGAAACTGCCCGCAAACCACCGGCTGCGGTGTTCTGAGGGGAAGCGCGAGACCTACGATTGGAGCCAACATAAACTTGGGGTCATTTTACTTGGTGGGAATGCCGGTGGCGATGCTGATGGGGTTCGTGGTGAAAATGGGGTTTGCAGGGCTGTGGCTTGGATTGCTTGCAGCTCAAGGCTCTTGTGCTTTGCTCATGTTTTATGTCCTTTGCACAACAGATTGGAATCTTCAGGTCAAAAGAGCAAATGATCTCACCCAAGCTTCTTCTGCTTCTACAACTTCTTCAATATTACCAATATCATCACCACGGCCACCACCAAGTTCAGATCACCAGGATCCCAACATGGAAGATGATGAGGTTGTCAAGTCATCAGAGGTGCTTGAAACAGACCCTCTTATTCACGTAGCGCTTGAAGCCCACTTCTCCTCTCATCAGCTTTCCTTATCTTCTTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0001101 GO:0003674 GO:0005215 GO:0005381 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005794 GO:0006810 GO:0006811 GO:0006812 GO:0006826 GO:0006855 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008324 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009605 GO:0009607 GO:0009624 GO:0009628 GO:0009642 GO:0009646 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0010015 GO:0010033 GO:0012505 GO:0015075 GO:0015238 GO:0015318 GO:0015893 GO:0017119 GO:0022603 GO:0022622 GO:0022857 GO:0022890 GO:0030001 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032991 GO:0033993 GO:0034220 GO:0034755 GO:0042221 GO:0042493 GO:0042592 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050789 GO:0050793 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0055085 GO:0065007 GO:0065008 GO:0097305 GO:0097708 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0099023 GO:0099402 GO:1901700 GO:1905392 GO:1905428

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MatE

Protein Analysis

565

Amino Acids

61.63

Weight (kDa)

6.07

Isoelectric Point (pI)

38.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MatE	PF01554	67 - 227	3.4e-37	MatE
MatE	PF01554	288 - 450	2.2e-35	MatE

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0015327)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G58340
fragaria_vesca	FvH4_5g33670
malus_domestica	MD08G1209800.v1.1 MD15G1393900.v1.1
pyrus_communis	pycom08g18100 pycom15g35380
rosa_chinensis	RchiOBHm_Chr7g0232641
rosa_laevigata	RLG00000001319
rosa_multiflora	Rmu_sc0005034.1_g000008
rosa_roxburghii	Rroxscaffold_3G00228070
rosa_rugosa	Rorug07G0275900
rosa_samantha	Rh7AG430200 Rh7BG403700 Rh7CG449100 Rh7DG419900
rosa_wichuraiana	Rw7G035540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	792
Acc36I	ACCTGC	1 cut(s)	875
AccB1I	GGYRCC	1 cut(s)	1149
AccB7I	CCANNNNNTGG	1 cut(s)	732
AccI	GTMKAC	1 cut(s)	990
AccII	CGCG	2 cut(s)	831, 1283
AccIII	TCCGGA	1 cut(s)	710
AciI	CCGC	9 cut(s)	288, 369, 604, 642, 829, 831, 921, 1247, 1263
AclWI	GGATC	2 cut(s)	1568, 1581
AcoI	YGGCCR	3 cut(s)	502, 717, 1547
AcsI	RAATTY	2 cut(s)	594, 692
AcuI	CTGAAG	2 cut(s)	401, 1451
AcyI	GRCGYC	1 cut(s)	1179
AfaI	GTAC	2 cut(s)	165, 776
AfeI	AGCGCT	2 cut(s)	817, 1647
AfiI	CCNNNNNNNGG	7 cut(s)	349, 368, 369, 468, 732, 1220, 1295
AflIII	ACRYGT	1 cut(s)	993
AgsI	TTSAA	6 cut(s)	116, 475, 957, 1524, 1622, 1652
AjnI	CCWGG	2 cut(s)	504, 1569
AjuI	GAANNNNNNNTTGG	1 cut(s)	36
AluBI	AGCT	9 cut(s)	133, 274, 280, 377, 400, 1195, 1409, 1499, 1675
AluI	AGCT	9 cut(s)	133, 274, 280, 377, 400, 1195, 1409, 1499, 1675
Alw21I	GWGCWC	3 cut(s)	135, 282, 1072
Alw26I	GTCTC	3 cut(s)	1027, 1093, 1279
AlwI	GGATC	2 cut(s)	1568, 1581
AlwNI	CAGNNNCTG	1 cut(s)	161
Aor13HI	TCCGGA	1 cut(s)	710
Aor51HI	AGCGCT	2 cut(s)	817, 1647
AoxI	GGCC	9 cut(s)	143, 214, 340, 384, 406, 502, 717, 1114, 1547
ApeKI	GCWGC	2 cut(s)	1260, 1406
ApoI	RAATTY	2 cut(s)	594, 692
AspLEI	GCGC	5 cut(s)	818, 853, 1052, 1283, 1648
AspS9I	GGNCC	2 cut(s)	197, 384
AsuHPI	GGTGA	5 cut(s)	281, 1378, 1483, 1533, 1559
AvaII	GGWCC	1 cut(s)	197
BalI	TGGCCA	2 cut(s)	504, 719
BamHI	GGATCC	1 cut(s)	1573
BanI	GGYRCC	1 cut(s)	1149
BanII	GRGCYC	2 cut(s)	135, 282
BauI	CACGAG	3 cut(s)	799, 1027, 1065
BbsI	GAAGAC	1 cut(s)	123
Bbv12I	GWGCWC	3 cut(s)	135, 282, 1072
BbvI	GCAGC	2 cut(s)	1247, 1418
BccI	CCATC	7 cut(s)	89, 135, 208, 371, 464, 653, 1348
BceAI	ACGGC	3 cut(s)	1004, 1215, 1562
BcgI	CGANNNNNNTGC	2 cut(s)	1192, 1226
BciT130I	CCWGG	2 cut(s)	506, 1571
BcoDI	GTCTC	3 cut(s)	1027, 1093, 1279
BfaI	CTAG	2 cut(s)	257, 683
BfoI	RGCGCY	3 cut(s)	819, 1053, 1649
BfuAI	ACCTGC	1 cut(s)	875
BglI	GCCNNNNNGGC	1 cut(s)	831
BglII	AGATCT	1 cut(s)	189
BisI	GCNGC	4 cut(s)	289, 832, 1261, 1407
BlpI	GCTNAGC	1 cut(s)	1010
BlsI	GCNGC	4 cut(s)	290, 833, 1262, 1408
Bme1390I	CCNGG	2 cut(s)	506, 1571
Bme18I	GGWCC	1 cut(s)	197
BmgT120I	GGNCC	2 cut(s)	197, 384
BmiI	GGNNCC	5 cut(s)	360, 385, 1151, 1300, 1575
BmrFI	CCNGG	2 cut(s)	506, 1571
BmsI	GCATC	2 cut(s)	879, 1338
BpiI	GAAGAC	1 cut(s)	123
BplI	GAGNNNNNCTC	4 cut(s)	477, 509, 506, 538
Bpu1102I	GCTNAGC	1 cut(s)	1010
BpuEI	CTTGAG	4 cut(s)	214, 296, 1175, 1395
BsaAI	YACGTR	3 cut(s)	774, 994, 1642
BsaBI	GATNNNNATC	1 cut(s)	1461
BsaHI	GRCGYC	1 cut(s)	1179
BsaI	GGTCTC	1 cut(s)	1279
BsaJI	CCNNGG	5 cut(s)	348, 403, 726, 829, 1544
BsaWI	WCCGGW	1 cut(s)	710
Bsc4I	CCNNNNNNNGG	7 cut(s)	349, 368, 369, 468, 732, 1220, 1295
Bse118I	RCCGGY	3 cut(s)	284, 1256, 1339
Bse1I	ACTGG	2 cut(s)	325, 626
Bse3DI	GCAATG	2 cut(s)	620, 1208
Bse8I	GATNNNNATC	1 cut(s)	1461
BseAI	TCCGGA	1 cut(s)	710
BseBI	CCWGG	2 cut(s)	506, 1571
BseDI	CCNNGG	5 cut(s)	348, 403, 726, 829, 1544
BseGI	GGATG	3 cut(s)	540, 663, 753
BseJI	GATNNNNATC	1 cut(s)	1461
BseLI	CCNNNNNNNGG	7 cut(s)	349, 368, 369, 468, 732, 1220, 1295
BseMI	GCAATG	2 cut(s)	620, 1208
BseMII	CTCAG	4 cut(s)	168, 207, 604, 1262
BseNI	ACTGG	2 cut(s)	325, 626
BseRI	GAGGAG	5 cut(s)	392, 428, 482, 647, 1654
BseXI	GCAGC	2 cut(s)	1247, 1418
BseYI	CCCAGC	1 cut(s)	812
Bsh1236I	CGCG	2 cut(s)	831, 1283
Bsh1285I	CGRYCG	1 cut(s)	1061
BshFI	GGCC	9 cut(s)	145, 216, 342, 386, 408, 504, 719, 1116, 1549
BshNI	GGYRCC	1 cut(s)	1149
BsiEI	CGRYCG	1 cut(s)	1061
BsiHKAI	GWGCWC	3 cut(s)	135, 282, 1072
BsiSI	CCGG	5 cut(s)	285, 339, 711, 1257, 1340
BslFI	GGGAC	1 cut(s)	795
BslI	CCNNNNNNNGG	7 cut(s)	349, 368, 369, 468, 732, 1220, 1295
BsmAI	GTCTC	3 cut(s)	1027, 1093, 1279
BsmBI	CGTCTC	2 cut(s)	1027, 1093
BsmFI	GGGAC	1 cut(s)	795
BsmI	GAATGC	1 cut(s)	1341
BsnI	GGCC	9 cut(s)	145, 216, 342, 386, 408, 504, 719, 1116, 1549
Bso31I	GGTCTC	1 cut(s)	1279
Bsp1286I	GDGCHC	3 cut(s)	135, 282, 1072
Bsp13I	TCCGGA	1 cut(s)	710
Bsp1407I	TGTACA	1 cut(s)	163
Bsp143I	GATC	6 cut(s)	189, 240, 1083, 1486, 1564, 1573
Bsp1720I	GCTNAGC	1 cut(s)	1010
Bsp19I	CCATGG	2 cut(s)	348, 726
BspACI	CCGC	9 cut(s)	288, 369, 604, 642, 829, 831, 921, 1247, 1263
BspANI	GGCC	9 cut(s)	145, 216, 342, 386, 408, 504, 719, 1116, 1549
BspCNI	CTCAG	4 cut(s)	167, 206, 603, 1263
BspEI	TCCGGA	1 cut(s)	710
BspFNI	CGCG	2 cut(s)	831, 1283
BspHI	TCATGA	1 cut(s)	906
BspLI	GGNNCC	5 cut(s)	360, 385, 1151, 1300, 1575
BspMI	ACCTGC	1 cut(s)	875
BspPI	GGATC	2 cut(s)	1568, 1581
BspQI	GCTCTTC	1 cut(s)	767
BspT107I	GGYRCC	1 cut(s)	1149
BspTNI	GGTCTC	1 cut(s)	1279
BsrDI	GCAATG	2 cut(s)	620, 1208
BsrFI	RCCGGY	3 cut(s)	284, 1256, 1339
BsrGI	TGTACA	1 cut(s)	163
BsrI	ACTGG	2 cut(s)	325, 626
BssAI	RCCGGY	3 cut(s)	284, 1256, 1339
BssECI	CCNNGG	5 cut(s)	348, 403, 726, 829, 1544
BssMI	GATC	6 cut(s)	189, 240, 1083, 1486, 1564, 1573
BssNI	GRCGYC	1 cut(s)	1179
BssSI	CACGAG	3 cut(s)	799, 1027, 1065
BssT1I	CCWWGG	2 cut(s)	348, 726
Bst2BI	CACGAG	3 cut(s)	799, 1027, 1065
Bst2UI	CCWGG	2 cut(s)	506, 1571
Bst4CI	ACNGT	4 cut(s)	98, 427, 927, 1062
Bst6I	CTCTTC	6 cut(s)	507, 551, 569, 582, 755, 767
BstACI	GRCGYC	1 cut(s)	1179
BstAUI	TGTACA	1 cut(s)	163
BstBAI	YACGTR	3 cut(s)	774, 994, 1642
BstC8I	GCNNGC	9 cut(s)	282, 286, 371, 375, 849, 855, 1072, 1247, 1404
BstDEI	CTNAG	5 cut(s)	154, 193, 590, 1010, 1271
BstDSI	CCRYGG	4 cut(s)	348, 726, 829, 1544
BstF5I	GGATG	3 cut(s)	540, 663, 753
BstFNI	CGCG	2 cut(s)	831, 1283
BstH2I	RGCGCY	3 cut(s)	819, 1053, 1649
BstHHI	GCGC	5 cut(s)	818, 853, 1052, 1283, 1648
BstKTI	GATC	6 cut(s)	192, 243, 1086, 1489, 1567, 1576
BstMAI	GTCTC	3 cut(s)	1027, 1093, 1279
BstMBI	GATC	6 cut(s)	189, 240, 1083, 1486, 1564, 1573
BstMCI	CGRYCG	1 cut(s)	1061
BstMWI	GCNNNNNNNGC	6 cut(s)	383, 822, 831, 1399, 1505, 1652
BstNI	CCWGG	2 cut(s)	506, 1571
BstSCI	CCNGG	2 cut(s)	504, 1569
BstUI	CGCG	2 cut(s)	831, 1283
BstV1I	GCAGC	2 cut(s)	1247, 1418
BstV2I	GAAGAC	1 cut(s)	123
BstX2I	RGATCY	2 cut(s)	189, 1573
BstXI	CCANNNNNNTGG	3 cut(s)	61, 355, 727
BstYI	RGATCY	2 cut(s)	189, 1573
BsuRI	GGCC	9 cut(s)	145, 216, 342, 386, 408, 504, 719, 1116, 1549
BtgI	CCRYGG	4 cut(s)	348, 726, 829, 1544
BtgZI	GCGATG	1 cut(s)	1361
BtsCI	GGATG	3 cut(s)	540, 663, 753
BtsIMutI	CAGTG	4 cut(s)	94, 318, 633, 1110
BveI	ACCTGC	1 cut(s)	875
Cac8I	GCNNGC	9 cut(s)	282, 286, 371, 375, 849, 855, 1072, 1247, 1404
CaiI	CAGNNNCTG	1 cut(s)	161
CciI	TCATGA	1 cut(s)	906
CfoI	GCGC	5 cut(s)	818, 853, 1052, 1283, 1648
Cfr10I	RCCGGY	3 cut(s)	284, 1256, 1339
Cfr13I	GGNCC	2 cut(s)	197, 384
Cfr42I	CCGCGG	1 cut(s)	832
CseI	GACGC	1 cut(s)	1187
Csp6I	GTAC	2 cut(s)	164, 775
CspCI	CAANNNNNGTGG	4 cut(s)	967, 1002, 1015, 1050
CviQI	GTAC	2 cut(s)	164, 775
DdeI	CTNAG	5 cut(s)	154, 193, 590, 1010, 1271
DpnI	GATC	6 cut(s)	191, 242, 1085, 1488, 1566, 1575
DpnII	GATC	6 cut(s)	189, 240, 1083, 1486, 1564, 1573
EaeI	YGGCCR	3 cut(s)	502, 717, 1547
Eam1104I	CTCTTC	6 cut(s)	507, 551, 569, 582, 755, 767
EarI	CTCTTC	6 cut(s)	507, 551, 569, 582, 755, 767
EciI	GGCGGA	1 cut(s)	631
Ecl136II	GAGCTC	2 cut(s)	133, 280
Eco130I	CCWWGG	2 cut(s)	348, 726
Eco147I	AGGCCT	2 cut(s)	216, 1116
Eco24I	GRGCYC	2 cut(s)	135, 282
Eco31I	GGTCTC	1 cut(s)	1279
Eco32I	GATATC	1 cut(s)	263
Eco47I	GGWCC	1 cut(s)	197
Eco47III	AGCGCT	2 cut(s)	817, 1647
Eco53kI	GAGCTC	2 cut(s)	133, 280
Eco57I	CTGAAG	2 cut(s)	401, 1451
EcoICRI	GAGCTC	2 cut(s)	133, 280
EcoRII	CCWGG	2 cut(s)	504, 1569
EcoRV	GATATC	1 cut(s)	263
EcoT14I	CCWWGG	2 cut(s)	348, 726
EcoT38I	GRGCYC	2 cut(s)	135, 282
ErhI	CCWWGG	2 cut(s)	348, 726
Esp3I	CGTCTC	2 cut(s)	1027, 1093
FaqI	GGGAC	1 cut(s)	795
FauI	CCCGC	2 cut(s)	362, 1254
FblI	GTMKAC	1 cut(s)	990
Fnu4HI	GCNGC	4 cut(s)	289, 832, 1261, 1407
FokI	GGATG	3 cut(s)	527, 650, 740
FriOI	GRGCYC	2 cut(s)	135, 282
Fsp4HI	GCNGC	4 cut(s)	289, 832, 1261, 1407
FspBI	CTAG	2 cut(s)	257, 683
GlaI	GCGC	5 cut(s)	817, 852, 1051, 1282, 1647
GluI	GCNGC	4 cut(s)	289, 832, 1261, 1407
GsaI	CCCAGC	1 cut(s)	816
HaeII	RGCGCY	3 cut(s)	819, 1053, 1649
HaeIII	GGCC	9 cut(s)	145, 216, 342, 386, 408, 504, 719, 1116, 1549
HapII	CCGG	5 cut(s)	285, 339, 711, 1257, 1340
HgaI	GACGC	1 cut(s)	1187
HhaI	GCGC	5 cut(s)	818, 853, 1052, 1283, 1648
Hin1I	GRCGYC	1 cut(s)	1179
Hin6I	GCGC	5 cut(s)	816, 851, 1050, 1281, 1646
HinP1I	GCGC	5 cut(s)	816, 851, 1050, 1281, 1646
HindIII	AAGCTT	2 cut(s)	375, 1497
HinfI	GANTC	4 cut(s)	486, 797, 1164, 1462
HpaII	CCGG	5 cut(s)	285, 339, 711, 1257, 1340
HphI	GGTGA	5 cut(s)	281, 1378, 1483, 1533, 1559
Hpy166II	GTNNAC	2 cut(s)	991, 1134
Hpy188I	TCNGA	8 cut(s)	103, 157, 420, 593, 1238, 1272, 1564, 1612
Hpy188III	TCNNGA	7 cut(s)	231, 557, 711, 862, 907, 973, 1065
Hpy8I	GTNNAC	2 cut(s)	991, 1134
Hpy99I	CGWCG	3 cut(s)	89, 125, 530
HpyAV	CCTTC	6 cut(s)	101, 425, 443, 472, 778, 1011
HpyCH4III	ACNGT	4 cut(s)	98, 427, 927, 1062
HpyCH4IV	ACGT	3 cut(s)	773, 993, 1641
HpyCH4V	TGCA	6 cut(s)	6, 870, 1208, 1382, 1406, 1449
HpyF10VI	GCNNNNNNNGC	6 cut(s)	383, 822, 831, 1399, 1505, 1652
HpyF3I	CTNAG	5 cut(s)	154, 193, 590, 1010, 1271
HpySE526I	ACGT	3 cut(s)	773, 993, 1641
Hsp92I	GRCGYC	1 cut(s)	1179
HspAI	GCGC	5 cut(s)	816, 851, 1050, 1281, 1646
Kpn2I	TCCGGA	1 cut(s)	710
KroI	GCCGGC	1 cut(s)	284
KroNI	GCCGGC	1 cut(s)	286
KspI	CCGCGG	1 cut(s)	832
Kzo9I	GATC	6 cut(s)	189, 240, 1083, 1486, 1564, 1573
LguI	GCTCTTC	1 cut(s)	767
LmnI	GCTCC	4 cut(s)	138, 405, 644, 1298
Lsp1109I	GCAGC	2 cut(s)	1247, 1418
LweI	GCATC	2 cut(s)	879, 1338
MaeI	CTAG	2 cut(s)	257, 683
MaeII	ACGT	3 cut(s)	773, 993, 1641
MaeIII	GTNAC	2 cut(s)	628, 1062
MalI	GATC	6 cut(s)	191, 242, 1085, 1488, 1566, 1575
MboI	GATC	6 cut(s)	189, 240, 1083, 1486, 1564, 1573
MfeI	CAATTG	1 cut(s)	300
MflI	RGATCY	2 cut(s)	189, 1573
MhlI	GDGCHC	3 cut(s)	135, 282, 1072
MlsI	TGGCCA	2 cut(s)	504, 719
MluCI	AATT	4 cut(s)	55, 300, 594, 692
MluNI	TGGCCA	2 cut(s)	504, 719
MlyI	GAGTC	2 cut(s)	495, 791
MmeI	TCCRAC	1 cut(s)	96
Mox20I	TGGCCA	2 cut(s)	504, 719
MreI	CGCCGGCG	1 cut(s)	284
MroI	TCCGGA	1 cut(s)	710
MroNI	GCCGGC	1 cut(s)	284
MscI	TGGCCA	2 cut(s)	504, 719
MseI	TTAA	1 cut(s)	690
MslI	CAYNNNNRTG	4 cut(s)	353, 725, 911, 1079
Msp20I	TGGCCA	2 cut(s)	504, 719
MspA1I	CMGCKG	1 cut(s)	831
MspI	CCGG	5 cut(s)	285, 339, 711, 1257, 1340
MspR9I	CCNGG	2 cut(s)	506, 1571
MunI	CAATTG	1 cut(s)	300
Mva1269I	GAATGC	1 cut(s)	1341
MvaI	CCWGG	2 cut(s)	506, 1571
MvnI	CGCG	2 cut(s)	831, 1283
MwoI	GCNNNNNNNGC	6 cut(s)	383, 822, 831, 1399, 1505, 1652
NaeI	GCCGGC	1 cut(s)	286
NcoI	CCATGG	2 cut(s)	348, 726
NdeII	GATC	6 cut(s)	189, 240, 1083, 1486, 1564, 1573
NgoMIV	GCCGGC	1 cut(s)	284
NlaIV	GGNNCC	5 cut(s)	360, 385, 1151, 1300, 1575
NmeAIII	GCCGAG	2 cut(s)	384, 1207
NmuCI	GTSAC	2 cut(s)	628, 1062
PagI	TCATGA	1 cut(s)	906
PceI	AGGCCT	2 cut(s)	216, 1116
PciSI	GCTCTTC	1 cut(s)	767
PcsI	WCGNNNNNNNCGW	1 cut(s)	1063
PctI	GAATGC	1 cut(s)	1341
PdiI	GCCGGC	1 cut(s)	286
PfeI	GAWTC	2 cut(s)	1164, 1462
PflMI	CCANNNNNTGG	1 cut(s)	732
PkrI	GCNGC	4 cut(s)	290, 833, 1262, 1408
PleI	GAGTC	2 cut(s)	494, 791
PpsI	GAGTC	2 cut(s)	494, 791
Ppu21I	YACGTR	3 cut(s)	774, 994, 1642
PsiI	TTATAA	1 cut(s)	792
Psp124BI	GAGCTC	2 cut(s)	135, 282
Psp6I	CCWGG	2 cut(s)	504, 1569
PspFI	CCCAGC	1 cut(s)	812
PspGI	CCWGG	2 cut(s)	504, 1569
PspN4I	GGNNCC	5 cut(s)	360, 385, 1151, 1300, 1575
PspPI	GGNCC	2 cut(s)	197, 384
PstNI	CAGNNNCTG	1 cut(s)	161
PsuI	RGATCY	2 cut(s)	189, 1573
RsaI	GTAC	2 cut(s)	165, 776
RsaNI	GTAC	2 cut(s)	164, 775
RseI	CAYNNNNRTG	4 cut(s)	353, 725, 911, 1079
SacI	GAGCTC	2 cut(s)	135, 282
SacII	CCGCGG	1 cut(s)	832
SapI	GCTCTTC	1 cut(s)	767
SaqAI	TTAA	1 cut(s)	690
SatI	GCNGC	4 cut(s)	289, 832, 1261, 1407
Sau3AI	GATC	6 cut(s)	189, 240, 1083, 1486, 1564, 1573
Sau96I	GGNCC	2 cut(s)	197, 384
SchI	GAGTC	2 cut(s)	495, 791
ScrFI	CCNGG	2 cut(s)	506, 1571
SduI	GDGCHC	3 cut(s)	135, 282, 1072
SfaNI	GCATC	2 cut(s)	879, 1338
Sfr303I	CCGCGG	1 cut(s)	832
SgrAI	CRCCGGYG	1 cut(s)	284
SgrBI	CCGCGG	1 cut(s)	832
SinI	GGWCC	1 cut(s)	197
SmiMI	CAYNNNNRTG	4 cut(s)	353, 725, 911, 1079
SmlI	CTYRAG	4 cut(s)	229, 275, 1190, 1410
SmoI	CTYRAG	4 cut(s)	229, 275, 1190, 1410
Sse9I	AATT	4 cut(s)	55, 300, 594, 692
SseBI	AGGCCT	2 cut(s)	216, 1116
SsiI	CCGC	9 cut(s)	288, 369, 604, 642, 829, 831, 921, 1247, 1263
SspI	AATATT	1 cut(s)	1527
SspMI	CTAG	2 cut(s)	257, 683
SstI	GAGCTC	2 cut(s)	135, 282
StuI	AGGCCT	2 cut(s)	216, 1116
StyD4I	CCNGG	2 cut(s)	504, 1569
StyI	CCWWGG	2 cut(s)	348, 726
TaaI	ACNGT	4 cut(s)	98, 427, 927, 1062
TaiI	ACGT	3 cut(s)	776, 996, 1644
TaqI	TCGA	3 cut(s)	84, 885, 1057
TasI	AATT	4 cut(s)	55, 300, 594, 692
TatI	WGTACW	1 cut(s)	163
TauI	GCSGC	2 cut(s)	291, 834
TfiI	GAWTC	2 cut(s)	1164, 1462
Tru1I	TTAA	1 cut(s)	690
Tru9I	TTAA	1 cut(s)	690
TscAI	CASTG	4 cut(s)	101, 325, 633, 1110
TseFI	GTSAC	2 cut(s)	628, 1062
TseI	GCWGC	2 cut(s)	1260, 1406
Tsp45I	GTSAC	2 cut(s)	628, 1062
TspDTI	ATGAA	7 cut(s)	49, 410, 454, 494, 529, 742, 895
TspGWI	ACGGA	2 cut(s)	514, 865
TspRI	CASTG	4 cut(s)	101, 325, 633, 1110
Van91I	CCANNNNNTGG	1 cut(s)	732
VpaK11BI	GGWCC	1 cut(s)	197
XapI	RAATTY	2 cut(s)	594, 692
XcmI	CCANNNNNNNNNTGG	2 cut(s)	955, 1309
XmiI	GTMKAC	1 cut(s)	990
XspI	CTAG	2 cut(s)	257, 683

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.