Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0011774

Physical Location & Seq

Genomic Coordinates Forward (+)

27392738 .. 27396527

Gene Structure 3790 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1210400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3072 bp

ATGCTTCTTGTTACTGCTGCACCCAATGATCCCCTCAATCATCACATTTATAAACCGCTTCTCTTCCTCTCCTCCTTCCCTGCCACAATTCCTATCAGTTTTTTAAAACTACCCTTTATTTCCCTCTCTCTAAAACCTTCTCTCTCTCTCTCTCTCTCAATCTTTGATTTTTCAAACATGGCTTCTTATAATCTTACCAGCTTCGTCCTTTCTATCTTGCTTATAATTCACTCGCTTTTTTATCCGTTTCAAGCCGTCATCAGTACAAATACTAACCAGTCTGAGTTTTTTGTGCTCATAATAAAGTCTGTTTCAGCTAACTCAGGAAACTCCCTGTCAGATTGGGATGTCACAGGAGGGAAACCATACTGCAACTTCTCCGGCGTCACCTGCAACAATGATGGGTATGTTCTCGAGCTTGATATCTCCGGTAGGTCTCTGTCCGGCAAGTTTCCTGCAGGAATCTGCTCTTACCTGCCTCAGTTGCGCGTTCTCCGTCTTGGACGTAACAATCTCCAGGGTGACTTTGTGGACAGCATCACCAACTGCTCATTCTTGGAAGAGGTTAACATGGATCATTTGACCCTCAGTCAGACGCTTCCGGATTTTTCACCCTTGAAATCTCTGAGGGTTCTTGACATGTCTTACAATTTGTTCAAAGGCAAGTTCCCCATGTCAGTGTTCAATCTCACCAATCTTGAGGTGCTCAACTTCAACGAAAACGGAGATTTCAACTTGTGGCAACTTCCAGATGACATCCATCGGCTGACAAAGCTCAATTCTATGATCTTGACAACGTGCATGATTCAAGGAAAGATCCCTCCATCAATAGGAAACATGACTTCTCTTGTTGATCTCGAATTGAGTGGCAATTACCTGGTTGGTAAAATTCCTGCGGAGCTTGGTTTGCTCAAGAATTTGAAACAGTTAGAACTCTACTACAACCTACTTGTTGGAACTATCCCTGAGGAGCTTGGAAATCTCACTGAACTCGAAGACTTAGACATGTCAGTCAATAAGCTGACTGGAAATATTCCAGAATCTATTTGTCGCCTTCCCAAGCTCCAAGTCCTGCAGTTTTACAACAACAGCCTTTCAGGTGAAATACCAAGCGCCATTGCAGACTCGAAAACACTAAGCATGCTGTCGCTGTACGATAACTTCCTGACAGGAGAAGTTCCAAGAAATCTGGGGCACTCATCATCCATGATTGTCCTGGACTTGTCCGAGAACCATCTTTCCGGTCCATTGCCAACGGAGGTTTGCAAGGGAGGTAAGCTGCTCTATTTTCTTATGCTGGACAATAAGTTATCTGGGGAGATACCAGAGAGCTACGCAGAATGCCAGTCTCTTCTTCGCTTTCGACTTAATGGTAATCATCTGGAGGGTTCAATACCTGCAGGACTTCTTAGTCTTCCCCATGTTTCGATCTTTGATTTGAGTTACAACAATTTGAGTGGTCAGATTGCAGATACAATTGGAAGAGCTAGAAACTTGTCAGAATTTTTTATACAAAGCAACAGGATTTCAGGTATTCTACCACCTGCAATCTCTGGAGCAATCAGCCTAGTGAAGATTGACCTCAGTAATAATTTTCTTTCTGGTCCAATTCCTTCTGAAATTGGCAACTTAAAAAAGCTAAATCTACTGATGTTGCAATGCAACAAGCTCAACTCTTCCATTCCGGATTCACTTTCTTCACTGAAATCTCTCAATGTTCTTGATGTCTCCAACAACCTCTTGACTGGAAAAATCCCAGAGAGTCTCAGTGAATTGTTACCAAACTCCATCAACTTCTCAAACAATAAGCTTTCTGGTCCAATTCCGCTCTCATTGATAAAAGGCGGGTTGGTGGAAAGCTTTTCAGGCAACCCACGCCTCTGTGTTAAAGTTTCTTCAGATCAAAATAATTTTCCCATATGTTCACAATCCTTGAACAAAAAGAAGTTGAATTCTTTCTGGGTTGTTATAGTTTCGGTAGTCCTCCTCCTCATTGGAGCTCTGCTGTTCCTAAAGCGCCGATTTGGAAAAGAAAGAGCAGAGGTGGAACATGATGAGACCCTGTCCTCATCATTCTTCTCATATGATGTAAAGAGCTTTCATCGAATAAGCTTCGACCACCGCGAGGTCATTGAAGCCATGGTTGATAAGAACATAGTAGGCCATGGAGGATCAGGGACAGTGTACAAGATTGAGCTGAGCCGCGGGGATGTGATTGCAGTGAAGAGGCTGTGGAGTAGAAAAGCAAAAGATTCGACAGAAGATCAGCCGTTCATCCACAAGGAGTTGAAAACTGAGGTGGAGACGCTGGGAAGTATCAGGCACAAAAACATTGTCAAATTGTACTGCTACTTCTCAAGCTTGGACTGCAACCTGTTGGTTTATGAGTACATGCCAAATGGTAACCTTTGGGATGCCCTTCACAAAGGATGGATCCATCTAGATTGGCCTACTCGTCACCAGATTGCGCTCGGGATTGCACAGGGTTTGGCATACCTCCACCATGATCTGATGCCTCCAATTATTCACAGAGATATCAAGTCTACCAACATCCTGCTTGACGTCAATAACCAAGCCAAGGTTGCAGATTTTGGCATAGCCAAGGTTTTACAAGCAAGTGGAGGAAAAGATTCCACCACCACTGTTATTGCTGGGACTTATGGTTACTTAGCCCCAGAATATGCATATTCATCCAAAGCAACAACCAAGTGTGACGTCTACAGTTTTGGGGTAGTTTTGATGGAGCTGATAACAGGGAAGAGGCCAGTGGAGGCAGAGTTTGGAGACAACAAGAACATCATATACTGGGTTTCAAACAAAGTGGACACCAAGGAAGGAGCCATGGAGGTGCTTGACAAGCGATTATCGGACTCACTCCAGGAGGAGATGATCCAGGTTCTTCGCATTGCCGTTCGATGTACCTATAAGGCGCCTTCGCTGCGTCCAAGCATGAAGGAGGTGGTTCAGCTGCTGATTGAAGCTGACCCTTGCAGATTTGATTCATGCAAGTCATCAACTAAGACCAAAGAAGCACCAAATGTGACAAAGGTCAAGAACCCTTATGAGTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001653 GO:0003002 GO:0003674 GO:0005488 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009962 GO:0009987 GO:0009991 GO:0010015 GO:0010051 GO:0010101 GO:0010102 GO:0010311 GO:0015698 GO:0015706 GO:0017046 GO:0019222 GO:0022622 GO:0023052 GO:0031323 GO:0031326 GO:0031537 GO:0031540 GO:0031667 GO:0032501 GO:0032502 GO:0033218 GO:0038023 GO:0042277 GO:0042562 GO:0042594 GO:0043455 GO:0044087 GO:0048364 GO:0048518 GO:0048527 GO:0048528 GO:0048580 GO:0048582 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051716 GO:0060089 GO:0065007 GO:0071705 GO:0080090 GO:0090548 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1900376 GO:1901141 GO:1901333 GO:1902025 GO:1903338 GO:1905392 GO:1905393 GO:2000023 GO:2000026 GO:2000069 GO:2000280 GO:2000652 GO:2000762

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1024

Amino Acids

113.45

Weight (kDa)

6.43

Isoelectric Point (pI)

36.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	202 - 313	7.8e-10	Leucine-rich repeat region
LRR_14	PF23598	319 - 509	4.1e-08	Leucine-rich repeat region
Pkinase	PF00069	717 - 989	2.7e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	718 - 990	2.2e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011774)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49660
fragaria_vesca	FvH4_5g33580 FvH4_5g33580
malus_domestica	MD08G1210400.v1.1 MD12G1134800.v1.1 MD15G1394300.v1.1
prunus_persica	Prupe.1G542400_v2.0.a1
pyrus_communis	pycom08g18140 pycom15g35440 pycom15g35450
rosa_chinensis	RchiOBHm_Chr7g0232421
rosa_laevigata	RLG00000001343
rosa_multiflora	Rmu_sc0003113.1_g000026
rosa_roxburghii	Rroxscaffold_3G00228340
rosa_samantha	Rh7AG427300 Rh7BG402200 Rh7CG446800 Rh7DG418800
rosa_wichuraiana	Rw7G035460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	51, 189, 224
AarI	CACCTGC	2 cut(s)	398, 1552
AasI	GACNNNNNNGTC	2 cut(s)	1010, 1410
AatII	GACGTC	2 cut(s)	2565, 2718
Acc36I	ACCTGC	4 cut(s)	398, 483, 1405, 1552
AccB1I	GGYRCC	1 cut(s)	2929
AccBSI	CCGCTC	1 cut(s)	1828
AccI	GTMKAC	2 cut(s)	2543, 2718
AccII	CGCG	3 cut(s)	489, 2124, 2205
AccIII	TCCGGA	2 cut(s)	601, 1684
AciI	CCGC	7 cut(s)	56, 896, 1826, 1845, 2122, 2203, 2205
AclWI	GGATC	7 cut(s)	23, 582, 811, 2179, 2428, 2441, 2884
AcsI	RAATTY	4 cut(s)	888, 916, 1502, 1951
AcuI	CTGAAG	1 cut(s)	1881
AcyI	GRCGYC	4 cut(s)	384, 2562, 2715, 2930
AfaI	GTAC	6 cut(s)	265, 1154, 2186, 2345, 2390, 2920
AfiI	CCNNNNNNNGG	3 cut(s)	830, 2125, 2578
AflIII	ACRYGT	2 cut(s)	639, 1005
AhdI	GACNNNNNGTC	1 cut(s)	588
AjnI	CCWGG	5 cut(s)	516, 876, 1215, 2877, 2892
AjuI	GAANNNNNNNTTGG	2 cut(s)	536, 568
AloI	GAACNNNNNNTCC	2 cut(s)	1223, 1255
Alw21I	GWGCWC	3 cut(s)	297, 708, 2002
Alw26I	GTCTC	7 cut(s)	441, 1353, 1732, 1769, 2051, 2297, 2778
AlwI	GGATC	7 cut(s)	23, 582, 811, 2179, 2428, 2441, 2884
AlwNI	CAGNNNCTG	1 cut(s)	2971
Ama87I	CYCGRG	2 cut(s)	413, 2471
Aor13HI	TCCGGA	2 cut(s)	601, 1684
AoxI	GGCC	3 cut(s)	2161, 2447, 2762
ApeKI	GCWGC	4 cut(s)	17, 1279, 2938, 2968
ApoI	RAATTY	4 cut(s)	888, 916, 1502, 1951
ArsI	GACNNNNNNTTYG	2 cut(s)	2319, 2351
Asp700I	GAANNNNTTC	2 cut(s)	1955, 2629
AspLEI	GCGC	5 cut(s)	489, 1115, 2019, 2470, 2932
AspS9I	GGNCC	3 cut(s)	1244, 1604, 1817
AsuHPI	GGTGA	7 cut(s)	379, 532, 533, 603, 682, 1112, 2450
AvaI	CYCGRG	2 cut(s)	413, 2471
AvaII	GGWCC	3 cut(s)	1244, 1604, 1817
AxyI	CCTNAGG	1 cut(s)	966
BaeGI	GKGCMC	1 cut(s)	1197
BamHI	GGATCC	1 cut(s)	2433
BanI	GGYRCC	1 cut(s)	2929
BanII	GRGCYC	1 cut(s)	2002
BarI	GAAGNNNNNNTAC	4 cut(s)	1145, 1177, 2336, 2368
BbsI	GAAGAC	2 cut(s)	1002, 1406
Bbv12I	GWGCWC	3 cut(s)	297, 708, 2002
BbvI	GCAGC	4 cut(s)	4, 1266, 2925, 2955
BccI	CCATC	8 cut(s)	395, 768, 832, 1242, 1796, 2424, 2445, 2734
BceAI	ACGGC	3 cut(s)	239, 2254, 2894
BcgI	CGANNNNNNTGC	2 cut(s)	2235, 2269
BciT130I	CCWGG	5 cut(s)	518, 878, 1217, 2879, 2894
BcoDI	GTCTC	7 cut(s)	441, 1353, 1732, 1769, 2051, 2297, 2778
BfaI	CTAG	3 cut(s)	1488, 1568, 2441
BfmI	CTRYAG	4 cut(s)	456, 1073, 1398, 2719
BfoI	RGCGCY	3 cut(s)	1116, 2020, 2933
BfuAI	ACCTGC	4 cut(s)	398, 483, 1405, 1552
BisI	GCNGC	5 cut(s)	18, 1280, 2203, 2939, 2969
BlpI	GCTNAGC	1 cut(s)	2198
BlsI	GCNGC	5 cut(s)	19, 1281, 2204, 2940, 2970
Bme1390I	CCNGG	5 cut(s)	518, 878, 1217, 2879, 2894
Bme18I	GGWCC	3 cut(s)	1244, 1604, 1817
BmeRI	GACNNNNNGTC	1 cut(s)	588
BmeT110I	CYCGRG	2 cut(s)	413, 2471
BmgT120I	GGNCC	3 cut(s)	1244, 1604, 1817
BmiI	GGNNCC	3 cut(s)	2435, 2839, 2931
BmrFI	CCNGG	5 cut(s)	518, 878, 1217, 2879, 2894
BmrI	ACTGGG	1 cut(s)	2815
BmsI	GCATC	3 cut(s)	546, 2404, 2502
BmuI	ACTGGG	1 cut(s)	2815
BoxI	GACNNNNGTC	1 cut(s)	3047
BpiI	GAAGAC	2 cut(s)	1002, 1406
BpmI	CTGGAG	4 cut(s)	500, 1403, 1575, 2861
Bpu1102I	GCTNAGC	1 cut(s)	2198
BpuEI	CTTGAG	3 cut(s)	719, 896, 2341
BsaHI	GRCGYC	4 cut(s)	384, 2562, 2715, 2930
BsaI	GGTCTC	2 cut(s)	441, 2051
BsaJI	CCNNGG	8 cut(s)	517, 2139, 2164, 2203, 2577, 2601, 2829, 2841
BsaWI	WCCGGW	4 cut(s)	428, 601, 1241, 1684
BsaXI	ACNNNNNCTCC	2 cut(s)	2762, 2792
Bsc4I	CCNNNNNNNGG	3 cut(s)	830, 2125, 2578
Bse1I	ACTGG	6 cut(s)	277, 1030, 1345, 1750, 2765, 2810
Bse21I	CCTNAGG	1 cut(s)	966
Bse3DI	GCAATG	4 cut(s)	1116, 1247, 1664, 2904
BseAI	TCCGGA	2 cut(s)	601, 1684
BseBI	CCWGG	5 cut(s)	518, 878, 1217, 2879, 2894
BseDI	CCNNGG	8 cut(s)	517, 2139, 2164, 2203, 2577, 2601, 2829, 2841
BseGI	GGATG	9 cut(s)	352, 756, 1202, 2215, 2274, 2419, 2435, 2550, 2690
BseLI	CCNNNNNNNGG	3 cut(s)	830, 2125, 2578
BseMI	GCAATG	4 cut(s)	1116, 1247, 1664, 2904
BseNI	ACTGG	6 cut(s)	277, 1030, 1345, 1750, 2765, 2810
BseRI	GAGGAG	5 cut(s)	61, 983, 1976, 1979, 2897
BseSI	GKGCMC	1 cut(s)	1197
BseXI	GCAGC	4 cut(s)	4, 1266, 2925, 2955
BseYI	CCCAGC	2 cut(s)	2308, 2651
Bsh1236I	CGCG	3 cut(s)	489, 2124, 2205
BshFI	GGCC	3 cut(s)	2163, 2449, 2764
BshNI	GGYRCC	1 cut(s)	2929
BsiHKAI	GWGCWC	3 cut(s)	297, 708, 2002
BsiHKCI	CYCGRG	2 cut(s)	413, 2471
BsiSI	CCGG	6 cut(s)	381, 429, 444, 602, 1242, 1685
BslFI	GGGAC	2 cut(s)	2191, 2668
BslI	CCNNNNNNNGG	3 cut(s)	830, 2125, 2578
BsmAI	GTCTC	7 cut(s)	441, 1353, 1732, 1769, 2051, 2297, 2778
BsmBI	CGTCTC	1 cut(s)	2297
BsmFI	GGGAC	2 cut(s)	2191, 2668
BsmI	GAATGC	1 cut(s)	1346
BsnI	GGCC	3 cut(s)	2163, 2449, 2764
Bso31I	GGTCTC	2 cut(s)	441, 2051
BsoBI	CYCGRG	2 cut(s)	413, 2471
Bsp1286I	GDGCHC	4 cut(s)	297, 708, 1197, 2002
Bsp13I	TCCGGA	2 cut(s)	601, 1684
Bsp1407I	TGTACA	1 cut(s)	2184
Bsp1720I	GCTNAGC	1 cut(s)	2198
Bsp19I	CCATGG	3 cut(s)	2139, 2164, 2841
BspACI	CCGC	7 cut(s)	56, 896, 1826, 1845, 2122, 2203, 2205
BspANI	GGCC	3 cut(s)	2163, 2449, 2764
BspEI	TCCGGA	2 cut(s)	601, 1684
BspFNI	CGCG	3 cut(s)	489, 2124, 2205
BspLI	GGNNCC	3 cut(s)	2435, 2839, 2931
BspMAI	CTGCAG	3 cut(s)	460, 1077, 1402
BspMI	ACCTGC	4 cut(s)	398, 483, 1405, 1552
BspPI	GGATC	7 cut(s)	23, 582, 811, 2179, 2428, 2441, 2884
BspQI	GCTCTTC	1 cut(s)	1477
BspT107I	GGYRCC	1 cut(s)	2929
BspTNI	GGTCTC	2 cut(s)	441, 2051
BsrBI	CCGCTC	1 cut(s)	1828
BsrDI	GCAATG	4 cut(s)	1116, 1247, 1664, 2904
BsrGI	TGTACA	1 cut(s)	2184
BsrI	ACTGG	6 cut(s)	277, 1030, 1345, 1750, 2765, 2810
BssECI	CCNNGG	8 cut(s)	517, 2139, 2164, 2203, 2577, 2601, 2829, 2841
BssNI	GRCGYC	4 cut(s)	384, 2562, 2715, 2930
BssT1I	CCWWGG	6 cut(s)	2139, 2164, 2577, 2601, 2829, 2841
Bst2UI	CCWGG	5 cut(s)	518, 878, 1217, 2879, 2894
Bst4CI	ACNGT	4 cut(s)	927, 2182, 2644, 2723
Bst6I	CTCTTC	7 cut(s)	68, 555, 1356, 1477, 1681, 2219, 2753
BstACI	GRCGYC	4 cut(s)	384, 2562, 2715, 2930
BstAUI	TGTACA	1 cut(s)	2184
BstC8I	GCNNGC	1 cut(s)	1142
BstDSI	CCRYGG	4 cut(s)	2139, 2164, 2203, 2841
BstEII	GGTNACC	1 cut(s)	2402
BstF5I	GGATG	9 cut(s)	352, 756, 1202, 2215, 2274, 2419, 2435, 2550, 2690
BstFNI	CGCG	3 cut(s)	489, 2124, 2205
BstH2I	RGCGCY	3 cut(s)	1116, 2020, 2933
BstHHI	GCGC	5 cut(s)	489, 1115, 2019, 2470, 2932
BstMAI	GTCTC	7 cut(s)	441, 1353, 1732, 1769, 2051, 2297, 2778
BstNI	CCWGG	5 cut(s)	518, 878, 1217, 2879, 2894
BstNSI	RCATGY	4 cut(s)	643, 1009, 1144, 2395
BstPAI	GACNNNNGTC	1 cut(s)	3047
BstPI	GGTNACC	1 cut(s)	2402
BstSCI	CCNGG	5 cut(s)	516, 876, 1215, 2877, 2892
BstSFI	CTRYAG	4 cut(s)	456, 1073, 1398, 2719
BstSLI	GKGCMC	1 cut(s)	1197
BstUI	CGCG	3 cut(s)	489, 2124, 2205
BstV1I	GCAGC	4 cut(s)	4, 1266, 2925, 2955
BstV2I	GAAGAC	2 cut(s)	1002, 1406
BstX2I	RGATCY	2 cut(s)	816, 2433
BstYI	RGATCY	2 cut(s)	816, 2433
Bsu36I	CCTNAGG	1 cut(s)	966
BsuRI	GGCC	3 cut(s)	2163, 2449, 2764
BtgI	CCRYGG	4 cut(s)	2139, 2164, 2203, 2841
BtsCI	GGATG	9 cut(s)	352, 756, 1202, 2215, 2274, 2419, 2435, 2550, 2690
BtsI	GCAGTG	1 cut(s)	2226
BtsIMutI	CAGTG	8 cut(s)	684, 984, 1700, 1774, 2187, 2226, 2640, 2772
BveI	ACCTGC	4 cut(s)	398, 483, 1405, 1552
Cac8I	GCNNGC	1 cut(s)	1142
CaiI	CAGNNNCTG	1 cut(s)	2971
CfoI	GCGC	5 cut(s)	489, 1115, 2019, 2470, 2932
Cfr13I	GGNCC	3 cut(s)	1244, 1604, 1817
Cfr42I	CCGCGG	1 cut(s)	2206
CseI	GACGC	4 cut(s)	373, 604, 2314, 2930
CsiI	ACCWGGT	1 cut(s)	876
Csp6I	GTAC	6 cut(s)	264, 1153, 2185, 2344, 2389, 2919
CspCI	CAANNNNNGTGG	6 cut(s)	1438, 1473, 2629, 2664, 2802, 2837
CviQI	GTAC	6 cut(s)	264, 1153, 2185, 2344, 2389, 2919
DinI	GGCGCC	1 cut(s)	2931
DraI	TTTAAA	1 cut(s)	105
DrdI	GACNNNNNNGTC	2 cut(s)	1010, 1410
DriI	GACNNNNNGTC	1 cut(s)	588
DseDI	GACNNNNNNGTC	2 cut(s)	1010, 1410
Eam1104I	CTCTTC	7 cut(s)	68, 555, 1356, 1477, 1681, 2219, 2753
Eam1105I	GACNNNNNGTC	1 cut(s)	588
EarI	CTCTTC	7 cut(s)	68, 555, 1356, 1477, 1681, 2219, 2753
Ecl136II	GAGCTC	1 cut(s)	2000
Eco130I	CCWWGG	6 cut(s)	2139, 2164, 2577, 2601, 2829, 2841
Eco24I	GRGCYC	1 cut(s)	2002
Eco31I	GGTCTC	2 cut(s)	441, 2051
Eco32I	GATATC	2 cut(s)	424, 2536
Eco47I	GGWCC	3 cut(s)	1244, 1604, 1817
Eco53kI	GAGCTC	1 cut(s)	2000
Eco57I	CTGAAG	1 cut(s)	1881
Eco81I	CCTNAGG	1 cut(s)	966
Eco88I	CYCGRG	2 cut(s)	413, 2471
Eco91I	GGTNACC	1 cut(s)	2402
EcoICRI	GAGCTC	1 cut(s)	2000
EcoO65I	GGTNACC	1 cut(s)	2402
EcoRI	GAATTC	1 cut(s)	1951
EcoRII	CCWGG	5 cut(s)	516, 876, 1215, 2877, 2892
EcoRV	GATATC	2 cut(s)	424, 2536
EcoT14I	CCWWGG	6 cut(s)	2139, 2164, 2577, 2601, 2829, 2841
EcoT22I	ATGCAT	1 cut(s)	2686
EcoT38I	GRGCYC	1 cut(s)	2002
EgeI	GGCGCC	1 cut(s)	2931
EheI	GGCGCC	1 cut(s)	2931
ErhI	CCWWGG	6 cut(s)	2139, 2164, 2577, 2601, 2829, 2841
Esp3I	CGTCTC	1 cut(s)	2297
FaqI	GGGAC	2 cut(s)	2191, 2668
FauI	CCCGC	2 cut(s)	1838, 2198
FauNDI	CATATG	2 cut(s)	1919, 2083
FblI	GTMKAC	2 cut(s)	2543, 2718
Fnu4HI	GCNGC	5 cut(s)	18, 1280, 2203, 2939, 2969
FokI	GGATG	9 cut(s)	359, 743, 1189, 2222, 2261, 2426, 2442, 2537, 2677
FriOI	GRGCYC	1 cut(s)	2002
Fsp4HI	GCNGC	5 cut(s)	18, 1280, 2203, 2939, 2969
FspBI	CTAG	3 cut(s)	1488, 1568, 2441
GlaI	GCGC	5 cut(s)	488, 1114, 2018, 2469, 2931
GluI	GCNGC	5 cut(s)	18, 1280, 2203, 2939, 2969
GsaI	CCCAGC	2 cut(s)	2312, 2655
GsuI	CTGGAG	4 cut(s)	500, 1403, 1575, 2861
HaeII	RGCGCY	3 cut(s)	1116, 2020, 2933
HaeIII	GGCC	3 cut(s)	2163, 2449, 2764
HapII	CCGG	6 cut(s)	381, 429, 444, 602, 1242, 1685
HgaI	GACGC	4 cut(s)	373, 604, 2314, 2930
HhaI	GCGC	5 cut(s)	489, 1115, 2019, 2470, 2932
Hin1I	GRCGYC	4 cut(s)	384, 2562, 2715, 2930
Hin6I	GCGC	5 cut(s)	487, 1113, 2017, 2468, 2930
HinP1I	GCGC	5 cut(s)	487, 1113, 2017, 2468, 2930
HincII	GTYRAC	1 cut(s)	568
HindII	GTYRAC	1 cut(s)	568
HindIII	AAGCTT	4 cut(s)	1808, 1858, 2110, 2359
HpaI	GTTAAC	1 cut(s)	568
HpaII	CCGG	6 cut(s)	381, 429, 444, 602, 1242, 1685
HphI	GGTGA	7 cut(s)	379, 532, 533, 603, 682, 1112, 2450
Hpy166II	GTNNAC	7 cut(s)	532, 568, 1925, 2185, 2544, 2719, 2824
Hpy8I	GTNNAC	7 cut(s)	532, 568, 1925, 2185, 2544, 2719, 2824
HpyAV	CCTTC	8 cut(s)	85, 147, 1064, 1623, 2429, 2828, 2943, 2947
HpyCH4III	ACNGT	4 cut(s)	927, 2182, 2644, 2723
HpyCH4IV	ACGT	4 cut(s)	505, 797, 2562, 2715
HpySE526I	ACGT	4 cut(s)	505, 797, 2562, 2715
Hsp92I	GRCGYC	4 cut(s)	384, 2562, 2715, 2930
HspAI	GCGC	5 cut(s)	487, 1113, 2017, 2468, 2930
KasI	GGCGCC	1 cut(s)	2929
Kpn2I	TCCGGA	2 cut(s)	601, 1684
KspAI	GTTAAC	1 cut(s)	568
KspI	CCGCGG	1 cut(s)	2206
LguI	GCTCTTC	1 cut(s)	1477
LmnI	GCTCC	7 cut(s)	898, 970, 1068, 1556, 1997, 2743, 2837
Lsp1109I	GCAGC	4 cut(s)	4, 1266, 2925, 2955
LweI	GCATC	3 cut(s)	546, 2404, 2502
MabI	ACCWGGT	1 cut(s)	876
MaeI	CTAG	3 cut(s)	1488, 1568, 2441
MaeII	ACGT	4 cut(s)	505, 797, 2562, 2715
MbiI	CCGCTC	1 cut(s)	1828
MfeI	CAATTG	1 cut(s)	1476
MflI	RGATCY	2 cut(s)	816, 2433
MhlI	GDGCHC	4 cut(s)	297, 708, 1197, 2002
Mly113I	GGCGCC	1 cut(s)	2930
MlyI	GAGTC	3 cut(s)	1118, 1771, 2864
MmeI	TCCRAC	2 cut(s)	934, 1755
Mph1103I	ATGCAT	1 cut(s)	2686
MroI	TCCGGA	2 cut(s)	601, 1684
MroXI	GAANNNNTTC	2 cut(s)	1955, 2629
MseI	TTAA	5 cut(s)	104, 567, 1368, 1631, 1887
MslI	CAYNNNNRTG	2 cut(s)	677, 2846
MspA1I	CMGCKG	2 cut(s)	2205, 2968
MspI	CCGG	6 cut(s)	381, 429, 444, 602, 1242, 1685
MspR9I	CCNGG	5 cut(s)	518, 878, 1217, 2879, 2894
MunI	CAATTG	1 cut(s)	1476
Mva1269I	GAATGC	1 cut(s)	1346
MvaI	CCWGG	5 cut(s)	518, 878, 1217, 2879, 2894
MvnI	CGCG	3 cut(s)	489, 2124, 2205
NarI	GGCGCC	1 cut(s)	2930
NcoI	CCATGG	3 cut(s)	2139, 2164, 2841
NdeI	CATATG	2 cut(s)	1919, 2083
NlaIV	GGNNCC	3 cut(s)	2435, 2839, 2931
NmuCI	GTSAC	6 cut(s)	349, 385, 521, 2456, 2711, 3040
NsiI	ATGCAT	1 cut(s)	2686
NspI	RCATGY	4 cut(s)	643, 1009, 1144, 2395
PaeI	GCATGC	1 cut(s)	1144
PaeR7I	CTCGAG	1 cut(s)	413
PaqCI	CACCTGC	2 cut(s)	398, 1552
PciI	ACATGT	2 cut(s)	639, 1005
PciSI	GCTCTTC	1 cut(s)	1477
PcsI	WCGNNNNNNNCGW	1 cut(s)	2121
PctI	GAATGC	1 cut(s)	1346
PdmI	GAANNNNTTC	2 cut(s)	1955, 2629
PfeI	GAWTC	7 cut(s)	462, 805, 1040, 1688, 2252, 2630, 2999
PflFI	GACNNNGTC	1 cut(s)	2062
PfoI	TCCNGGA	2 cut(s)	1215, 2877
PkrI	GCNGC	5 cut(s)	19, 1281, 2204, 2940, 2970
PleI	GAGTC	3 cut(s)	1118, 1770, 2864
PluTI	GGCGCC	1 cut(s)	2933
PpsI	GAGTC	3 cut(s)	1118, 1770, 2864
PscI	ACATGT	2 cut(s)	639, 1005
PshAI	GACNNNNGTC	1 cut(s)	3047
PsiI	TTATAA	3 cut(s)	51, 189, 224
Psp124BI	GAGCTC	1 cut(s)	2002
Psp6I	CCWGG	5 cut(s)	516, 876, 1215, 2877, 2892
PspEI	GGTNACC	1 cut(s)	2402
PspFI	CCCAGC	2 cut(s)	2308, 2651
PspGI	CCWGG	5 cut(s)	516, 876, 1215, 2877, 2892
PspN4I	GGNNCC	3 cut(s)	2435, 2839, 2931
PspPI	GGNCC	3 cut(s)	1244, 1604, 1817
PsrI	GAACNNNNNNTAC	2 cut(s)	2786, 2818
PstI	CTGCAG	3 cut(s)	460, 1077, 1402
PstNI	CAGNNNCTG	1 cut(s)	2971
PsuI	RGATCY	2 cut(s)	816, 2433
PsyI	GACNNNGTC	1 cut(s)	2062
PvuII	CAGCTG	1 cut(s)	2968
RsaI	GTAC	6 cut(s)	265, 1154, 2186, 2345, 2390, 2920
RsaNI	GTAC	6 cut(s)	264, 1153, 2185, 2344, 2389, 2919
RseI	CAYNNNNRTG	2 cut(s)	677, 2846
SacI	GAGCTC	1 cut(s)	2002
SacII	CCGCGG	1 cut(s)	2206
SapI	GCTCTTC	1 cut(s)	1477
SaqAI	TTAA	5 cut(s)	104, 567, 1368, 1631, 1887
SatI	GCNGC	5 cut(s)	18, 1280, 2203, 2939, 2969
Sau96I	GGNCC	3 cut(s)	1244, 1604, 1817
SbfI	CCTGCAGG	2 cut(s)	460, 1402
SchI	GAGTC	3 cut(s)	1118, 1771, 2864
ScrFI	CCNGG	5 cut(s)	518, 878, 1217, 2879, 2894
SdaI	CCTGCAGG	2 cut(s)	460, 1402
SduI	GDGCHC	4 cut(s)	297, 708, 1197, 2002
SexAI	ACCWGGT	1 cut(s)	876
SfaNI	GCATC	3 cut(s)	546, 2404, 2502
SfcI	CTRYAG	4 cut(s)	456, 1073, 1398, 2719
SfoI	GGCGCC	1 cut(s)	2931
Sfr274I	CTCGAG	1 cut(s)	413
Sfr303I	CCGCGG	1 cut(s)	2206
SgrBI	CCGCGG	1 cut(s)	2206
SinI	GGWCC	3 cut(s)	1244, 1604, 1817
SlaI	CTCGAG	1 cut(s)	413
SmiMI	CAYNNNNRTG	2 cut(s)	677, 2846
SmlI	CTYRAG	4 cut(s)	413, 698, 911, 2356
SmoI	CTYRAG	4 cut(s)	413, 698, 911, 2356
SphI	GCATGC	1 cut(s)	1144
Sse8387I	CCTGCAGG	2 cut(s)	460, 1402
SsiI	CCGC	7 cut(s)	56, 896, 1826, 1845, 2122, 2203, 2205
SspDI	GGCGCC	1 cut(s)	2929
SspI	AATATT	1 cut(s)	1033
SspMI	CTAG	3 cut(s)	1488, 1568, 2441
SstI	GAGCTC	1 cut(s)	2002
StyD4I	CCNGG	5 cut(s)	516, 876, 1215, 2877, 2892
StyI	CCWWGG	6 cut(s)	2139, 2164, 2577, 2601, 2829, 2841
TaaI	ACNGT	4 cut(s)	927, 2182, 2644, 2723
TaiI	ACGT	4 cut(s)	508, 800, 2565, 2718
TatI	WGTACW	4 cut(s)	263, 2184, 2343, 2388
TauI	GCSGC	1 cut(s)	2205
TfiI	GAWTC	7 cut(s)	462, 805, 1040, 1688, 2252, 2630, 2999
Tru1I	TTAA	5 cut(s)	104, 567, 1368, 1631, 1887
Tru9I	TTAA	5 cut(s)	104, 567, 1368, 1631, 1887
TscAI	CASTG	8 cut(s)	684, 991, 1707, 1774, 2187, 2226, 2647, 2772
TseFI	GTSAC	6 cut(s)	349, 385, 521, 2456, 2711, 3040
TseI	GCWGC	4 cut(s)	17, 1279, 2938, 2968
Tsp45I	GTSAC	6 cut(s)	349, 385, 521, 2456, 2711, 3040
TspDTI	ATGAA	5 cut(s)	2090, 2263, 2679, 2966, 2991
TspGWI	ACGGA	4 cut(s)	234, 485, 738, 1271
TspRI	CASTG	8 cut(s)	684, 991, 1707, 1774, 2187, 2226, 2647, 2772
Tth111I	GACNNNGTC	1 cut(s)	2062
VpaK11BI	GGWCC	3 cut(s)	1244, 1604, 1817
XapI	RAATTY	4 cut(s)	888, 916, 1502, 1951
XbaI	TCTAGA	1 cut(s)	2440
XceI	RCATGY	4 cut(s)	643, 1009, 1144, 2395
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1213
XhoI	CTCGAG	1 cut(s)	413
XmiI	GTMKAC	2 cut(s)	2543, 2718
XmnI	GAANNNNTTC	2 cut(s)	1955, 2629
XspI	CTAG	3 cut(s)	1488, 1568, 2441
ZraI	GACGTC	2 cut(s)	2563, 2716
Zsp2I	ATGCAT	1 cut(s)	2686

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.