Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0011774

Physical Location & Seq

Genomic Coordinates Reverse (-)

52918866 .. 52922744

Gene Structure 3879 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G035460.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2898 bp

ATGGCCTCTCACATAACCTACTTCCTTTCTGTGTTACTTATAATATTCTTGGTTTTGTATCCTTCTGAAGCCTTGATCACTAGTAGTAACCAGTCTCAGTTTTTTGTGCAAATAATAAAGTCTCTTTCACCTAATTCGGGTAGCTCTTTGTCAGATTGGGATGTCAAAGGAGGAAAACCTTACTGTAACTTCTCTGGGGTTAGCTGCAATGATGATGGCTACGTTGTCCGGATTGATATTTCCGGTCGATCACTCTCAGGTCACTTCCCAGCAGACATATGCTCCTACCTGCCTCAGTTGCGCATTCTCCGTCTTGGTCACAACAACCTCCAGGGTGACTTTGTTGATAGCATCACCAACTGCTCATTCTTGGAAGAGCTCAGCATGGATCATTTGTATCTCAGTGGAACTCTACCAGATTTTTCACCCTTGAAGAATCTAAAGATACTTGACTTGTCTTACAACAAGTTCAAAGGCAAGTTCCCCATGTCAGTGTTTAATCTTACCAATCTTGAGGTGCTCAATTTCAATGAGAATGGAGATTTCAACTTATGGCAGCTGCCGGAGAATATACACACACTGACAAAGCTCAAATCCATGGTCTTAACAACATGCATGGTACAAGGGAAGATCCCAACATCAATAGGAAACATGACTTCCCTTGTTGATCTTGAACTGAGTGGAAATTACCTGGTGGGTCAAATTCCAACAGAGATTGGATTGCTCAAGAATTTGAAGCAATTGGAGCTGTACTACAACCAACTCACTGGTAACATACCTGAGGAGCTCGGAAATCTCACAGACCTCACTGATATGGACATGTCAGTCAATAAGTTGACTGGGAAGATTCCAGAGTCAATCTGTCGGCTTCCCCGGCTTCAAGTCCTGCAGCTTTACAACAACAGCCTTTCAGGAGAGATCCCAACTGTGATTGCAGACTCAAAAACACTGAGCATGCTGTCACTTTATGACAATTTCCTGACTGGAGAAGTTCCAAGAAATCTGGGGAAATCATCAGCTATAGTTGTCCTCGACTTGTCAGAGAACCAACTTTCTGGTCCATTACCAACAGAGGTTTGCAGGGGAGGTAAGTTGCTCTACTTTCTTATTCTGGAAAACCAGTTTTCTGGAGAAATACCAGACAGCTATGCAGGATGTGAATCTCTTCTTCGATTTCGGCTTAGCTTTAATCGTTTGGAGGGCTCGATACCTGCAGGACTTCTTAATCTTCCCCATGTTTCCATCTTTGATCTGGCTTACAACAATCTGAGTGGTCAGATTGCTGATACAATTGGGAGAGCCAGAAATTTGTCTGAACTTTTTATCCAAAACAACAGCATTTCAGGTGTTCTGCCCCCTGGAATCTCTGGAGCAATAAGTCTAGTGAAGATTGATCTCAGTAATAATCTTATTTCTGGTCCAATTCCTTCAGAAATTGGAAAATTAAAGAAGCTGAATTTACTGATGTTGCAAGGCAACAAGCTCACTTCTTCCATTCCTGATTCACTTTCTTTGTTGAAATCACTCAATGTTCTTGATCTCTCCAACAACCTCTTGACAGGAAATATCCCTGATAGTCTTTGTGAATTGTTACCAAACTCTATCAACTTCTCAAACAATAAGCTTTCAGGTCCTATTCCAGTCCATTTGATAGAAGGAGGGCTAATAGAAAGCTTTTCTGGCAACCCTGGCCTTTGTGTGAAAGTCTATGTTAACTCATCTGATCAAAATAGGTTTCCCGTATGCCCACAACACTTCAACAGAAAGAAAATAAATTCCTTTTGGGTAGTAACAGTTTCAGTAGTTATTATTCTTATTGGAGCTATTCTCTTTCTGAAGCGCCGATTTGGAAAAGAAAGAGCAGAAGTGGAACATGAAGAATCTCTATCCTCATCATTCTTCTGCTATGATGTGAAAAGTTTTCATCGAATAAGTTTTGACCATCGTGAGGTCATTGAAGCTATGGTTGACAAGAACATAGTAGGCAATGGAGGATCTGGGACAGTTTACAAGATCGAGTTGAGCAGTGGGGACGTAGTTGCAGTGAAGAGACTATGGAGTAAGAAAACAAAAGAATCTTCAGAAGATGATCAGTTTGTTATACACAAGGAGTTGAAAACTGAGGTAGAGACTCTGGGTAACATAAGGCATAAGAACATTGTAAAATTGTTTTGCTACTTCTCAAGTTTGGATTGCAACCTGTTGGTTTATGAGTATATGCCGAACGGAAACCTTTGGGATGCTCTTCACAAAGGTTGGATCCATTTGGAGTGGCCTACTCGTCACCAGATCGCGCTAGGGATTGCACAGGGCTTGTCATATCTCCACCATGATCTGATGCCTCCAATTATTCACAGAGATATTAAGTCCACCAACATACTGCTTGATGTGAATTACCAACCCAAAGTTGCAGATTTTGGCATAGCAAAGGTTTTACAAGCAAGAGGAGGGAAAGATTCCACAACCACTGTTATCGCTGGGACATACGGTTACTTAGCCCCAGAATATGCATATTCATCCAAAGCTACAACCAAGTGCGATGTCTACAGTTTTGGGGTAGTTCTAATGGAACTGATAACTGGGAAGAAGCCGGTGGAGGCAGAGTTTGGTGAAAACAAGAACATCATATACTGGGTTTCAAATAAAGTGGATACTAAAGAAGGAGCCATGGAGGTTTTAGACAAGCGATTGTCAGAGTCATTCAAGGAGGAGATGATCCAAGTTCTTCGAATTGCCGTTCGATGTACCTACAAGGCCCCTTCTCTGCGTCCAACCATGAAGGAAGTGGTTCAGTTGCTGATTGAGGCAGACCCTTGCAGATTTGATTCATGCAAGTCATCAACCAAGACCAAAGAAGCATCAAATGTAACAAAGGTTAAGAACCCTTTTGAGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001653 GO:0003002 GO:0003674 GO:0005488 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009962 GO:0009987 GO:0009991 GO:0010015 GO:0010051 GO:0010101 GO:0010102 GO:0010311 GO:0015698 GO:0015706 GO:0017046 GO:0019222 GO:0022622 GO:0023052 GO:0031323 GO:0031326 GO:0031537 GO:0031540 GO:0031667 GO:0032501 GO:0032502 GO:0033218 GO:0038023 GO:0042277 GO:0042562 GO:0042594 GO:0043455 GO:0044087 GO:0048364 GO:0048518 GO:0048527 GO:0048528 GO:0048580 GO:0048582 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051716 GO:0060089 GO:0065007 GO:0071705 GO:0080090 GO:0090548 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1900376 GO:1901141 GO:1901333 GO:1902025 GO:1903338 GO:1905392 GO:1905393 GO:2000023 GO:2000026 GO:2000069 GO:2000280 GO:2000652 GO:2000762

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

965

Amino Acids

107.23

Weight (kDa)

6.29

Isoelectric Point (pI)

36.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	29 - 70	5.2e-06	Leucine rich repeat N-terminal domain
LRR_14	PF23598	137 - 253	8.8e-11	Leucine-rich repeat region
LRR_14	PF23598	238 - 448	6.9e-08	Leucine-rich repeat region
Pkinase	PF00069	658 - 931	7.2e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	659 - 932	9.6e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011774)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49660
fragaria_vesca	FvH4_5g33580 FvH4_5g33580
malus_domestica	MD08G1210400.v1.1 MD12G1134800.v1.1 MD15G1394300.v1.1
prunus_persica	Prupe.1G542400_v2.0.a1
pyrus_communis	pycom08g18140 pycom15g35440 pycom15g35450
rosa_chinensis	RchiOBHm_Chr7g0232421
rosa_laevigata	RLG00000001343
rosa_multiflora	Rmu_sc0003113.1_g000026
rosa_roxburghii	Rroxscaffold_3G00228340
rosa_samantha	Rh7AG427300 Rh7BG402200 Rh7CG446800 Rh7DG418800
rosa_wichuraiana	Rw7G035460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	41
AasI	GACNNNNNNGTC	1 cut(s)	824
Acc16I	TGCGCA	1 cut(s)	302
Acc36I	ACCTGC	2 cut(s)	297, 1219
AccI	GTMKAC	1 cut(s)	2544
AccII	CGCG	1 cut(s)	2294
AccIII	TCCGGA	1 cut(s)	228
AclWI	GGATC	7 cut(s)	396, 625, 913, 2002, 2254, 2267, 2710
AcsI	RAATTY	5 cut(s)	702, 730, 1306, 1456, 1774
AcuI	CTGAAG	4 cut(s)	87, 1413, 1856, 2064
AfaI	GTAC	3 cut(s)	621, 752, 2746
AfiI	CCNNNNNNNGG	2 cut(s)	137, 1948
AflIII	ACRYGT	1 cut(s)	819
AhlI	ACTAGT	1 cut(s)	80
AjnI	CCWGG	4 cut(s)	330, 690, 1357, 1687
AjuI	GAANNNNNNNTTGG	2 cut(s)	350, 382
AloI	GAACNNNNNNTCC	2 cut(s)	1308, 1340
Alw21I	GWGCWC	3 cut(s)	381, 522, 789
Alw26I	GTCTC	4 cut(s)	99, 126, 2044, 2123
AlwI	GGATC	7 cut(s)	396, 625, 913, 2002, 2254, 2267, 2710
AlwNI	CAGNNNCTG	1 cut(s)	2797
Aor13HI	TCCGGA	1 cut(s)	228
AoxI	GGCC	4 cut(s)	3, 1690, 2273, 2754
ApeKI	GCWGC	4 cut(s)	204, 556, 559, 889
ApoI	RAATTY	5 cut(s)	702, 730, 1306, 1456, 1774
ArsI	GACNNNNNNTTYG	2 cut(s)	585, 617
Asp700I	GAANNNNTTC	2 cut(s)	1164, 2787
AspLEI	GCGC	3 cut(s)	303, 1842, 2296
AspS9I	GGNCC	4 cut(s)	1058, 1418, 1631, 2755
AsuC2I	CCSGG	1 cut(s)	874
AsuHPI	GGTGA	6 cut(s)	120, 346, 347, 417, 2276, 2621
AsuII	TTCGAA	1 cut(s)	2728
AvaII	GGWCC	3 cut(s)	1058, 1418, 1631
AxyI	CCTNAGG	1 cut(s)	780
BamHI	GGATCC	1 cut(s)	2259
BanII	GRGCYC	3 cut(s)	381, 789, 1205
Bbv12I	GWGCWC	3 cut(s)	381, 522, 789
BbvI	GCAGC	4 cut(s)	191, 546, 568, 901
BccI	CCATC	3 cut(s)	209, 1250, 1950
BceAI	ACGGC	1 cut(s)	2720
BciT130I	CCWGG	4 cut(s)	332, 692, 1359, 1689
BciVI	GTATCC	2 cut(s)	69, 2644
BclI	TGATCA	3 cut(s)	75, 1723, 2089
BcnI	CCSGG	1 cut(s)	874
BcoDI	GTCTC	4 cut(s)	99, 126, 2044, 2123
BcuI	ACTAGT	1 cut(s)	80
BfaI	CTAG	3 cut(s)	81, 1382, 2297
BfmI	CTRYAG	4 cut(s)	887, 1020, 1212, 2545
BfoI	RGCGCY	1 cut(s)	1843
BfuAI	ACCTGC	2 cut(s)	297, 1219
BfuI	GTATCC	2 cut(s)	69, 2644
BisI	GCNGC	4 cut(s)	205, 557, 560, 890
BlpI	GCTNAGC	2 cut(s)	380, 1181
BlsI	GCNGC	4 cut(s)	206, 558, 561, 891
Bme1390I	CCNGG	5 cut(s)	332, 692, 874, 1359, 1689
Bme18I	GGWCC	3 cut(s)	1058, 1418, 1631
BmgT120I	GGNCC	4 cut(s)	1058, 1418, 1631, 2755
BmiI	GGNNCC	3 cut(s)	2261, 2665, 2757
BmrFI	CCNGG	5 cut(s)	332, 692, 874, 1359, 1689
BmrI	ACTGGG	3 cut(s)	849, 2589, 2641
BmsI	GCATC	4 cut(s)	360, 2230, 2328, 2867
BmuI	ACTGGG	3 cut(s)	849, 2589, 2641
BplI	GAGNNNNNCTC	2 cut(s)	1812, 1844
BpmI	CTGGAG	4 cut(s)	314, 1005, 1149, 1389
Bpu1102I	GCTNAGC	2 cut(s)	380, 1181
Bpu14I	TTCGAA	1 cut(s)	2728
BpuEI	CTTGAG	3 cut(s)	533, 710, 2167
BpuMI	CCSGG	1 cut(s)	874
BsaBI	GATNNNNATC	1 cut(s)	1159
BsaJI	CCNNGG	6 cut(s)	331, 597, 872, 1357, 1687, 2667
BsaWI	WCCGGW	2 cut(s)	228, 242
BsaXI	ACNNNNNCTCC	4 cut(s)	266, 296, 2588, 2618
Bsc4I	CCNNNNNNNGG	2 cut(s)	137, 1948
Bse118I	RCCGGY	1 cut(s)	2590
Bse1I	ACTGG	8 cut(s)	91, 772, 844, 988, 1120, 1640, 2584, 2636
Bse21I	CCTNAGG	1 cut(s)	780
Bse3DI	GCAATG	2 cut(s)	214, 1993
Bse8I	GATNNNNATC	1 cut(s)	1159
BseAI	TCCGGA	1 cut(s)	228
BseBI	CCWGG	4 cut(s)	332, 692, 1359, 1689
BseDI	CCNNGG	6 cut(s)	331, 597, 872, 1357, 1687, 2667
BseGI	GGATG	4 cut(s)	166, 1160, 2245, 2516
BseJI	GATNNNNATC	1 cut(s)	1159
BseLI	CCNNNNNNNGG	2 cut(s)	137, 1948
BseMI	GCAATG	2 cut(s)	214, 1993
BseNI	ACTGG	8 cut(s)	91, 772, 844, 988, 1120, 1640, 2584, 2636
BseRI	GAGGAG	3 cut(s)	797, 2460, 2723
BseXI	GCAGC	4 cut(s)	191, 546, 568, 901
BseYI	CCCAGC	2 cut(s)	268, 2477
Bsh1236I	CGCG	1 cut(s)	2294
Bsh1285I	CGRYCG	1 cut(s)	247
BshFI	GGCC	4 cut(s)	5, 1692, 2275, 2756
BsiEI	CGRYCG	1 cut(s)	247
BsiHKAI	GWGCWC	3 cut(s)	381, 522, 789
BsiSI	CCGG	5 cut(s)	229, 243, 563, 874, 2591
BslFI	GGGAC	3 cut(s)	2014, 2045, 2494
BslI	CCNNNNNNNGG	2 cut(s)	137, 1948
BsmAI	GTCTC	4 cut(s)	99, 126, 2044, 2123
BsmFI	GGGAC	3 cut(s)	2014, 2045, 2494
BsmI	GAATGC	1 cut(s)	303
BsnI	GGCC	4 cut(s)	5, 1692, 2275, 2756
Bsp119I	TTCGAA	1 cut(s)	2728
Bsp1286I	GDGCHC	4 cut(s)	381, 522, 789, 1205
Bsp13I	TCCGGA	1 cut(s)	228
Bsp1720I	GCTNAGC	2 cut(s)	380, 1181
Bsp19I	CCATGG	2 cut(s)	597, 2667
BspANI	GGCC	4 cut(s)	5, 1692, 2275, 2756
BspEI	TCCGGA	1 cut(s)	228
BspFNI	CGCG	1 cut(s)	2294
BspLI	GGNNCC	3 cut(s)	2261, 2665, 2757
BspMAI	CTGCAG	2 cut(s)	891, 1216
BspMI	ACCTGC	2 cut(s)	297, 1219
BspPI	GGATC	7 cut(s)	396, 625, 913, 2002, 2254, 2267, 2710
BspQI	GCTCTTC	2 cut(s)	369, 2250
BspT104I	TTCGAA	1 cut(s)	2728
BsrDI	GCAATG	2 cut(s)	214, 1993
BsrFI	RCCGGY	1 cut(s)	2590
BsrI	ACTGG	8 cut(s)	91, 772, 844, 988, 1120, 1640, 2584, 2636
BssAI	RCCGGY	1 cut(s)	2590
BssECI	CCNNGG	6 cut(s)	331, 597, 872, 1357, 1687, 2667
BssT1I	CCWWGG	2 cut(s)	597, 2667
Bst2UI	CCWGG	4 cut(s)	332, 692, 1359, 1689
Bst4CI	ACNGT	7 cut(s)	185, 928, 1795, 2005, 2470, 2489, 2549
Bst6I	CTCTTC	4 cut(s)	369, 1170, 2042, 2250
BstBI	TTCGAA	1 cut(s)	2728
BstC8I	GCNNGC	1 cut(s)	956
BstDSI	CCRYGG	2 cut(s)	597, 2667
BstF5I	GGATG	4 cut(s)	166, 1160, 2245, 2516
BstFNI	CGCG	1 cut(s)	2294
BstH2I	RGCGCY	1 cut(s)	1843
BstHHI	GCGC	3 cut(s)	303, 1842, 2296
BstMAI	GTCTC	4 cut(s)	99, 126, 2044, 2123
BstMCI	CGRYCG	1 cut(s)	247
BstMWI	GCNNNNNNNGC	5 cut(s)	298, 745, 874, 1680, 1689
BstNI	CCWGG	4 cut(s)	332, 692, 1359, 1689
BstNSI	RCATGY	3 cut(s)	615, 823, 958
BstSCI	CCNGG	5 cut(s)	330, 690, 872, 1357, 1687
BstSFI	CTRYAG	4 cut(s)	887, 1020, 1212, 2545
BstUI	CGCG	1 cut(s)	2294
BstV1I	GCAGC	4 cut(s)	191, 546, 568, 901
BstX2I	RGATCY	4 cut(s)	630, 918, 1994, 2259
BstXI	CCANNNNNNTGG	3 cut(s)	767, 1055, 1127
BstYI	RGATCY	4 cut(s)	630, 918, 1994, 2259
Bsu36I	CCTNAGG	1 cut(s)	780
BsuI	GTATCC	2 cut(s)	69, 2644
BsuRI	GGCC	4 cut(s)	5, 1692, 2275, 2756
BtgI	CCRYGG	2 cut(s)	597, 2667
BtgZI	GCGATG	1 cut(s)	2553
BtsCI	GGATG	4 cut(s)	166, 1160, 2245, 2516
BtsI	GCAGTG	2 cut(s)	2032, 2049
BtsIMutI	CAGTG	9 cut(s)	409, 498, 578, 765, 807, 947, 2032, 2049, 2466
BveI	ACCTGC	2 cut(s)	297, 1219
Cac8I	GCNNGC	1 cut(s)	956
CaiI	CAGNNNCTG	1 cut(s)	2797
CfoI	GCGC	3 cut(s)	303, 1842, 2296
Cfr10I	RCCGGY	1 cut(s)	2590
Cfr13I	GGNCC	4 cut(s)	1058, 1418, 1631, 2755
CseI	GACGC	1 cut(s)	2756
CsiI	ACCWGGT	1 cut(s)	690
Csp6I	GTAC	3 cut(s)	620, 751, 2745
CspCI	CAANNNNNGTGG	4 cut(s)	1252, 1287, 2628, 2663
CviQI	GTAC	3 cut(s)	620, 751, 2745
DrdI	GACNNNNNNGTC	1 cut(s)	824
DseDI	GACNNNNNNGTC	1 cut(s)	824
Eam1104I	CTCTTC	4 cut(s)	369, 1170, 2042, 2250
EarI	CTCTTC	4 cut(s)	369, 1170, 2042, 2250
Ecl136II	GAGCTC	2 cut(s)	379, 787
Eco130I	CCWWGG	2 cut(s)	597, 2667
Eco24I	GRGCYC	3 cut(s)	381, 789, 1205
Eco47I	GGWCC	3 cut(s)	1058, 1418, 1631
Eco53kI	GAGCTC	2 cut(s)	379, 787
Eco57I	CTGAAG	4 cut(s)	87, 1413, 1856, 2064
Eco81I	CCTNAGG	1 cut(s)	780
EcoICRI	GAGCTC	2 cut(s)	379, 787
EcoO109I	RGGNCCY	2 cut(s)	1631, 2755
EcoRII	CCWGG	4 cut(s)	330, 690, 1357, 1687
EcoT14I	CCWWGG	2 cut(s)	597, 2667
EcoT22I	ATGCAT	2 cut(s)	617, 2512
EcoT38I	GRGCYC	3 cut(s)	381, 789, 1205
ErhI	CCWWGG	2 cut(s)	597, 2667
FaqI	GGGAC	3 cut(s)	2014, 2045, 2494
FauNDI	CATATG	1 cut(s)	278
FbaI	TGATCA	3 cut(s)	75, 1723, 2089
FblI	GTMKAC	1 cut(s)	2544
Fnu4HI	GCNGC	4 cut(s)	205, 557, 560, 890
FokI	GGATG	4 cut(s)	173, 1167, 2252, 2503
FriOI	GRGCYC	3 cut(s)	381, 789, 1205
Fsp4HI	GCNGC	4 cut(s)	205, 557, 560, 890
FspBI	CTAG	3 cut(s)	81, 1382, 2297
FspI	TGCGCA	1 cut(s)	302
GlaI	GCGC	3 cut(s)	302, 1841, 2295
GluI	GCNGC	4 cut(s)	205, 557, 560, 890
GsaI	CCCAGC	2 cut(s)	272, 2481
GsuI	CTGGAG	4 cut(s)	314, 1005, 1149, 1389
HaeII	RGCGCY	1 cut(s)	1843
HaeIII	GGCC	4 cut(s)	5, 1692, 2275, 2756
HapII	CCGG	5 cut(s)	229, 243, 563, 874, 2591
HgaI	GACGC	1 cut(s)	2756
HhaI	GCGC	3 cut(s)	303, 1842, 2296
Hin6I	GCGC	3 cut(s)	301, 1840, 2294
HinP1I	GCGC	3 cut(s)	301, 1840, 2294
HincII	GTYRAC	3 cut(s)	837, 1714, 1969
HindII	GTYRAC	3 cut(s)	837, 1714, 1969
HindIII	AAGCTT	2 cut(s)	1622, 1672
HpaI	GTTAAC	1 cut(s)	1714
HpaII	CCGG	5 cut(s)	229, 243, 563, 874, 2591
HphI	GGTGA	6 cut(s)	120, 346, 347, 417, 2276, 2621
Hpy166II	GTNNAC	6 cut(s)	837, 1714, 1969, 2008, 2370, 2545
Hpy8I	GTNNAC	6 cut(s)	837, 1714, 1969, 2008, 2370, 2545
HpyAV	CCTTC	6 cut(s)	72, 1437, 1649, 2654, 2769, 2773
HpyCH4III	ACNGT	7 cut(s)	185, 928, 1795, 2005, 2470, 2489, 2549
HpyCH4IV	ACGT	2 cut(s)	222, 2034
HpyF10VI	GCNNNNNNNGC	5 cut(s)	298, 745, 874, 1680, 1689
HpySE526I	ACGT	2 cut(s)	222, 2034
HspAI	GCGC	3 cut(s)	301, 1840, 2294
Kpn2I	TCCGGA	1 cut(s)	228
Ksp22I	TGATCA	3 cut(s)	75, 1723, 2089
KspAI	GTTAAC	1 cut(s)	1714
LguI	GCTCTTC	2 cut(s)	369, 2250
LmnI	GCTCC	6 cut(s)	287, 745, 784, 1370, 1820, 2663
Lsp1109I	GCAGC	4 cut(s)	191, 546, 568, 901
LweI	GCATC	4 cut(s)	360, 2230, 2328, 2867
MabI	ACCWGGT	1 cut(s)	690
MaeI	CTAG	3 cut(s)	81, 1382, 2297
MaeII	ACGT	2 cut(s)	222, 2034
MfeI	CAATTG	2 cut(s)	740, 1290
MflI	RGATCY	4 cut(s)	630, 918, 1994, 2259
MhlI	GDGCHC	4 cut(s)	381, 522, 789, 1205
MlyI	GAGTC	4 cut(s)	863, 932, 2125, 2705
MmeI	TCCRAC	4 cut(s)	731, 1569, 2237, 2795
Mph1103I	ATGCAT	2 cut(s)	617, 2512
MroI	TCCGGA	1 cut(s)	228
MroXI	GAANNNNTTC	2 cut(s)	1164, 2787
MseI	TTAA	8 cut(s)	498, 605, 1188, 1224, 1445, 1713, 2364, 2877
MslI	CAYNNNNRTG	2 cut(s)	491, 812
MspA1I	CMGCKG	1 cut(s)	559
MspI	CCGG	5 cut(s)	229, 243, 563, 874, 2591
MspR9I	CCNGG	5 cut(s)	332, 692, 874, 1359, 1689
MunI	CAATTG	2 cut(s)	740, 1290
Mva1269I	GAATGC	1 cut(s)	303
MvaI	CCWGG	4 cut(s)	332, 692, 1359, 1689
MvnI	CGCG	1 cut(s)	2294
MwoI	GCNNNNNNNGC	5 cut(s)	298, 745, 874, 1680, 1689
NciI	CCSGG	1 cut(s)	874
NcoI	CCATGG	2 cut(s)	597, 2667
NdeI	CATATG	1 cut(s)	278
NlaIV	GGNNCC	3 cut(s)	2261, 2665, 2757
NmuCI	GTSAC	5 cut(s)	260, 317, 335, 960, 2282
NsbI	TGCGCA	1 cut(s)	302
NsiI	ATGCAT	2 cut(s)	617, 2512
NspI	RCATGY	3 cut(s)	615, 823, 958
NspV	TTCGAA	1 cut(s)	2728
PaeI	GCATGC	1 cut(s)	958
PciI	ACATGT	1 cut(s)	819
PciSI	GCTCTTC	2 cut(s)	369, 2250
PctI	GAATGC	1 cut(s)	303
PdmI	GAANNNNTTC	2 cut(s)	1164, 2787
PfeI	GAWTC	9 cut(s)	436, 847, 1160, 1362, 1502, 1880, 2075, 2456, 2825
PkrI	GCNGC	4 cut(s)	206, 558, 561, 891
PleI	GAGTC	4 cut(s)	862, 932, 2125, 2704
PpsI	GAGTC	4 cut(s)	862, 932, 2125, 2704
PpuMI	RGGWCCY	1 cut(s)	1631
PscI	ACATGT	1 cut(s)	819
PsiI	TTATAA	1 cut(s)	41
Psp124BI	GAGCTC	2 cut(s)	381, 789
Psp5II	RGGWCCY	1 cut(s)	1631
Psp6I	CCWGG	4 cut(s)	330, 690, 1357, 1687
PspFI	CCCAGC	2 cut(s)	268, 2477
PspGI	CCWGG	4 cut(s)	330, 690, 1357, 1687
PspN4I	GGNNCC	3 cut(s)	2261, 2665, 2757
PspPI	GGNCC	4 cut(s)	1058, 1418, 1631, 2755
PspPPI	RGGWCCY	1 cut(s)	1631
PsrI	GAACNNNNNNTAC	2 cut(s)	2612, 2644
PstI	CTGCAG	2 cut(s)	891, 1216
PstNI	CAGNNNCTG	1 cut(s)	2797
PsuI	RGATCY	4 cut(s)	630, 918, 1994, 2259
PvuII	CAGCTG	1 cut(s)	559
RsaI	GTAC	3 cut(s)	621, 752, 2746
RsaNI	GTAC	3 cut(s)	620, 751, 2745
RseI	CAYNNNNRTG	2 cut(s)	491, 812
SacI	GAGCTC	2 cut(s)	381, 789
SapI	GCTCTTC	2 cut(s)	369, 2250
SaqAI	TTAA	8 cut(s)	498, 605, 1188, 1224, 1445, 1713, 2364, 2877
SatI	GCNGC	4 cut(s)	205, 557, 560, 890
Sau96I	GGNCC	4 cut(s)	1058, 1418, 1631, 2755
SbfI	CCTGCAGG	1 cut(s)	1216
SchI	GAGTC	4 cut(s)	863, 932, 2125, 2705
ScrFI	CCNGG	5 cut(s)	332, 692, 874, 1359, 1689
SdaI	CCTGCAGG	1 cut(s)	1216
SduI	GDGCHC	4 cut(s)	381, 522, 789, 1205
SexAI	ACCWGGT	1 cut(s)	690
SfaNI	GCATC	4 cut(s)	360, 2230, 2328, 2867
SfcI	CTRYAG	4 cut(s)	887, 1020, 1212, 2545
SfuI	TTCGAA	1 cut(s)	2728
SinI	GGWCC	3 cut(s)	1058, 1418, 1631
SmiMI	CAYNNNNRTG	2 cut(s)	491, 812
SmlI	CTYRAG	3 cut(s)	512, 725, 2182
SmoI	CTYRAG	3 cut(s)	512, 725, 2182
SpeI	ACTAGT	1 cut(s)	80
SphI	GCATGC	1 cut(s)	958
Sse8387I	CCTGCAGG	1 cut(s)	1216
SspI	AATATT	1 cut(s)	45
SspMI	CTAG	3 cut(s)	81, 1382, 2297
SstI	GAGCTC	2 cut(s)	381, 789
StyD4I	CCNGG	5 cut(s)	330, 690, 872, 1357, 1687
StyI	CCWWGG	2 cut(s)	597, 2667
TaaI	ACNGT	7 cut(s)	185, 928, 1795, 2005, 2470, 2489, 2549
TaiI	ACGT	2 cut(s)	225, 2037
TaqI	TCGA	8 cut(s)	247, 1032, 1171, 1205, 1927, 2016, 2728, 2740
TatI	WGTACW	1 cut(s)	750
TfiI	GAWTC	9 cut(s)	436, 847, 1160, 1362, 1502, 1880, 2075, 2456, 2825
Tru1I	TTAA	8 cut(s)	498, 605, 1188, 1224, 1445, 1713, 2364, 2877
Tru9I	TTAA	8 cut(s)	498, 605, 1188, 1224, 1445, 1713, 2364, 2877
TscAI	CASTG	9 cut(s)	409, 498, 585, 772, 814, 954, 2032, 2049, 2473
TseFI	GTSAC	5 cut(s)	260, 317, 335, 960, 2282
TseI	GCWGC	4 cut(s)	204, 556, 559, 889
Tsp45I	GTSAC	5 cut(s)	260, 317, 335, 960, 2282
TspDTI	ATGAA	5 cut(s)	1890, 1913, 2505, 2792, 2817
TspGWI	ACGGA	2 cut(s)	299, 2241
TspRI	CASTG	9 cut(s)	409, 498, 585, 772, 814, 954, 2032, 2049, 2473
VpaK11BI	GGWCC	3 cut(s)	1058, 1418, 1631
XapI	RAATTY	5 cut(s)	702, 730, 1306, 1456, 1774
XceI	RCATGY	3 cut(s)	615, 823, 958
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1249, 2782
XmiI	GTMKAC	1 cut(s)	2544
XmnI	GAANNNNTTC	2 cut(s)	1164, 2787
XspI	CTAG	3 cut(s)	81, 1382, 2297
Zsp2I	ATGCAT	2 cut(s)	617, 2512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.