Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011774

Physical Location & Seq

Genomic Coordinates Forward (+)

49225994 .. 49229525

Gene Structure 3532 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1394300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2892 bp

ATGGCTTCTTATAATCATACCACCTTCGTCCTTTCTATCTTCCTTATAATTTACTCGCTTTTTAATCCGTCTCAAGCCGTCATCCGTACAAATACTAGCCAGTCTGAGTTTTTTTTGCTCATAATAAAGTCTGTTTCAGCAAACGCCGGGAACTCCCTGTCAGATTGGGATGTCAAAGGAGGGAAACCTTACTGCAACTTCTCCGGTGTCACCTGCAACAACGAAGGGTATGTTGTCGAGCTTGATATATCCAGTAGGTCACTCTCCGGTAACTTTCCTGCAGGCATCTGCTCTTACCTGCCTCAGTTGCGTGTTCTCCGTCTTGGACGTAACAATCTCCGGGGTGACTTTGTGGACAGCATCACCAACTGCTCATTCTTGGAAGAGCTCAACATGGAACATTTGTACCTCAGTCAGACGCTTCCGGATTTTTCACCCATGAAATCTCTGAAGGTTCTTGACATGTCGTACAATTTGTTCATCGGCAAGTTCCCCATGTCAGTGTTCAACCTCACCAATCTTGAGGTACTCAACTTCAATGAAAACGAAGGTTTCAACTTGTGGCAGCTTCCGGAGGACATCACTCGGCTGACAAATCTCAAATCTATGATCTTGACAACGTGCATGATTGAAGGGAAGATCCCGGCATCAATTGGAAAAATGACTTCTCTTGTTGATCTCGAATTGAGCGGCAATTTCCTGGGCGGTGAAATTCCGGCCGAGATTGGTTTGCTCAAGAATTTGAAACAGTTGGAGCTCTACTACAACCAACTTGTTGGAACTATACCTGAGGAGCTTGGAAATCTCACTGGCCTGGTAGAATTAGACATGTCAGTCAATAAGCTGACTGGAAGGATTCCGGAATCTATTTGTCGTCTTCCCAAGCTCCAAGTCCTGCAGCTTTACAACAACAGTCTTTCAGGTGAAATCCCAAGCGCCATCGCAGACTCGAAAACACTGAGCATGCTGTCGCTTTATGACAACTTCCTGACAGGAGAAATTCCTCGAAATCTGGGGCACTCATCACCAATGATTGTCTTAGACTTGTCCGAGAACCATTTTTCCGGTCCATTGCCAACGGAGGTTTGCAAGGGAGGTAAGTTGCTCTACTTTCTTATGCTGGACAATAAGTTATCTGGAGAGATACCAGAGAGCTACTCAGAATGCCAGTCTCTTCTTCGATTTCGACTTAGCTATAATCATCTGGAGGGCTCAATACCTGCAGGACTTCTTAGTCTTCCCCATGTTTCGATCTTTGATTTGGGTTACAATAATTTGAGTGGTCAGATTGCAGATACAATTGGAAGAGCTAGAAACTTGTCAGAATTGTTTATACAAAGCAACAAGATTTCAGGTGTTCTACCACCTGCAGTCTCTGGAGCAATCAGCCTAGTAAAGATTGACCTCAGTAATAATCTTCTTTCTGGTCCAATTCCTTCTGCAATTGGAAACTTAAAGAAGCTTAATTTACTGATGTTACAAGGCAACAAGCTCAACTCTTCCATTCCCGATTCTCTTTCTTCACTAAAATCTCTCAATGTTCTTGATCTCTCCAACAACCTCTTGACCGGAAACATCCCAGAGAGTCTCGTTGAATTATTACCAAACTCCATCAACTTCTCAAACAATAAGCTTTCTGGTCCAATTCCTCTCTCATTGATAAAAGGCGGGTTGGTGGAAAGCTTTTCCGGCAACCCAGGCCTCTGTGTTAAAGTTTCATCAGATCAAAATAATTTTCCCATATGTTCACAATCTCTTAACAAAAAAAAGTTAAATTCTTTCTGGGTAGTTACAGTTTCAGTAGTCTTCCTCCTCATTGGAGCTCTGCTGTTCCTAAAGCGCCGGTTTGGAAGAAGAGCAGAGGTGGAACATGATGAGAGCCTGTCCTCATCATTCTTCTCATATGATGTAAAGAGCTTCCATCGAATAAGCTTCGACCACCGCAAGGTCATTGAAGGCATGGTTGATAAGAACATAGTAGGCCATGGAGGATCAGGGACAGTGTACAAGATTGAGCTGAGCAGCGGGGATGTGATTGCAGTGAAGAGGCTGTGGAGTAGAAAAGCAAAAGATTCTGGAGAAGATCAGCTGTTCACACACAAGGAGTTGAAAACTGAGGTCGAGACGCTGGGAAGTATAAGGCACAAAAACATTGTCAAATTGTACTGCTACTTCTCAAGCTCGGACTGCAATCTTTTGGTTTATGAGTATATGCCAAATGGTAACCTTTGGGATGCTCTTCACAAAGGTTGGATCCATCTAGATTGGCCTACTCGTCACCAGATTGCCCTCGGGATTGCGCAGGGTTTGGCATACCTCCACCATGATCTGATGCCTCCAATTATTCACAGAGATATCAAGTCTACCAACATCCTGCTCGATGTCAATAACCAACCCAAGGTTGCAGATTTTGGCATAGCCAAGGTTTTACAAGCAAGAGGAGGAAAAGATTCCACCACCACTGTTATTGCTGGGACTTATGGTTACTTAGCCCCGGAATATGCATATTCATCCAAAGCAACAACCAAGTGTGACGTCTACAGTTTTGGGGTAGTTTTGATGGAGCTGATAACTGGGAAGAAGCCGGTGGAGGCAGAGTTTGGAGACAACAAGAACATCATATACTGGGTTTCAAACAAAGTGGACACCAAAGAAGGAGCTACGGAGGTGCTGGACAAGCGATCATCGGACTCATTCAAGGAGGAGATGATCCAGGTTCTTCGCATTGCCGTTCGATGCACCTACAAGGCGCCTTCGCTGCGCCCGAGCATGAAGGAGGTCGTTCAGCTGCTGATTGAAGCTGACCCTTGCAGATTTGATTCATGCAAGTCATCAACTAAGACCAAAGAAGCACCAAATGTGACAAAGGTCAAGAACCCTTATGAGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001653 GO:0003002 GO:0003674 GO:0005488 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009962 GO:0009987 GO:0009991 GO:0010015 GO:0010051 GO:0010101 GO:0010102 GO:0010311 GO:0015698 GO:0015706 GO:0017046 GO:0019222 GO:0022622 GO:0023052 GO:0031323 GO:0031326 GO:0031537 GO:0031540 GO:0031667 GO:0032501 GO:0032502 GO:0033218 GO:0038023 GO:0042277 GO:0042562 GO:0042594 GO:0043455 GO:0044087 GO:0048364 GO:0048518 GO:0048527 GO:0048528 GO:0048580 GO:0048582 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051716 GO:0060089 GO:0065007 GO:0071705 GO:0080090 GO:0090548 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1900376 GO:1901141 GO:1901333 GO:1902025 GO:1903338 GO:1905392 GO:1905393 GO:2000023 GO:2000026 GO:2000069 GO:2000280 GO:2000652 GO:2000762

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

964

Amino Acids

106.54

Weight (kDa)

6.82

Isoelectric Point (pI)

37.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	120 - 255	6e-10	Leucine-rich repeat region
LRR_14	PF23598	241 - 452	1.2e-09	Leucine-rich repeat region
LRR_8	PF13855	438 - 498	1e-06	Leucine rich repeat
LRR_8	PF13855	463 - 522	6.3e-07	Leucine rich repeat
Pkinase	PF00069	657 - 929	8.9e-49	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	658 - 930	1.4e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011774)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49660
fragaria_vesca	FvH4_5g33580 FvH4_5g33580
malus_domestica	MD08G1210400.v1.1 MD12G1134800.v1.1 MD15G1394300.v1.1
prunus_persica	Prupe.1G542400_v2.0.a1
pyrus_communis	pycom08g18140 pycom15g35440 pycom15g35450
rosa_chinensis	RchiOBHm_Chr7g0232421
rosa_laevigata	RLG00000001343
rosa_multiflora	Rmu_sc0003113.1_g000026
rosa_roxburghii	Rroxscaffold_3G00228340
rosa_samantha	Rh7AG427300 Rh7BG402200 Rh7CG446800 Rh7DG418800
rosa_wichuraiana	Rw7G035460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	12, 47
AarI	CACCTGC	2 cut(s)	221, 1375
AasI	GACNNNNNNGTC	2 cut(s)	833, 1233
AatII	GACGTC	1 cut(s)	2538
Acc16I	TGCGCA	1 cut(s)	2301
Acc36I	ACCTGC	4 cut(s)	221, 306, 1228, 1375
AccB1I	GGYRCC	1 cut(s)	2749
AccBSI	CCGCTC	1 cut(s)	690
AccI	GTMKAC	2 cut(s)	2363, 2538
AccIII	TCCGGA	3 cut(s)	424, 571, 859
AciI	CCGC	5 cut(s)	690, 705, 1668, 1942, 2025
AclWI	GGATC	5 cut(s)	634, 1999, 2248, 2261, 2704
AcoI	YGGCCR	1 cut(s)	717
AcsI	RAATTY	4 cut(s)	711, 739, 999, 1774
AcuI	CTGAAG	1 cut(s)	470
AcyI	GRCGYC	2 cut(s)	2535, 2750
AfaI	GTAC	6 cut(s)	88, 407, 470, 528, 2006, 2165
AfiI	CCNNNNNNNGG	2 cut(s)	1945, 2398
AflIII	ACRYGT	2 cut(s)	462, 828
AjnI	CCWGG	4 cut(s)	699, 813, 1696, 2712
AjuI	GAANNNNNNNTTGG	2 cut(s)	359, 391
AloI	GAACNNNNNNTCC	2 cut(s)	1046, 1078
Alw21I	GWGCWC	3 cut(s)	390, 759, 1825
Alw26I	GTCTC	6 cut(s)	75, 1176, 1378, 1592, 2117, 2598
AlwI	GGATC	5 cut(s)	634, 1999, 2248, 2261, 2704
AlwNI	CAGNNNCTG	2 cut(s)	1376, 2791
Ama87I	CYCGRG	2 cut(s)	2291, 2764
Aor13HI	TCCGGA	3 cut(s)	424, 571, 859
AoxI	GGCC	5 cut(s)	717, 811, 1699, 1981, 2267
ApeKI	GCWGC	5 cut(s)	565, 898, 2022, 2758, 2788
ApoI	RAATTY	4 cut(s)	711, 739, 999, 1774
ArsI	GACNNNNNNTTYG	2 cut(s)	2139, 2171
Asp700I	GAANNNNTTC	1 cut(s)	2449
AspLEI	GCGC	5 cut(s)	938, 1842, 2302, 2752, 2763
AspS9I	GGNCC	3 cut(s)	1067, 1427, 1640
AsuC2I	CCSGG	4 cut(s)	148, 341, 644, 2495
AsuHPI	GGTGA	9 cut(s)	202, 355, 356, 426, 505, 719, 935, 1017, 2270
AvaI	CYCGRG	2 cut(s)	2291, 2764
AvaII	GGWCC	3 cut(s)	1067, 1427, 1640
AxyI	CCTNAGG	1 cut(s)	789
BaeGI	GKGCMC	1 cut(s)	1020
BaeI	ACNNNNGTAYC	2 cut(s)	389, 422
BamHI	GGATCC	1 cut(s)	2253
BanI	GGYRCC	1 cut(s)	2749
BanII	GRGCYC	4 cut(s)	390, 759, 1214, 1825
BarI	GAAGNNNNNNTAC	2 cut(s)	2156, 2188
BbsI	GAAGAC	3 cut(s)	869, 1229, 1798
Bbv12I	GWGCWC	3 cut(s)	390, 759, 1825
BbvI	GCAGC	5 cut(s)	577, 910, 2034, 2745, 2775
BccI	CCATC	5 cut(s)	947, 1619, 1929, 2265, 2554
BceAI	ACGGC	2 cut(s)	62, 2714
BciT130I	CCWGG	4 cut(s)	701, 815, 1698, 2714
BcnI	CCSGG	4 cut(s)	148, 341, 644, 2495
BcoDI	GTCTC	6 cut(s)	75, 1176, 1378, 1592, 2117, 2598
BfaI	CTAG	4 cut(s)	96, 1311, 1391, 2261
BfmI	CTRYAG	5 cut(s)	279, 896, 1221, 1368, 2539
BfoI	RGCGCY	3 cut(s)	939, 1843, 2753
BfuAI	ACCTGC	4 cut(s)	221, 306, 1228, 1375
BisI	GCNGC	6 cut(s)	566, 691, 899, 2023, 2759, 2789
BlpI	GCTNAGC	1 cut(s)	2018
BlsI	GCNGC	6 cut(s)	567, 692, 900, 2024, 2760, 2790
Bme1390I	CCNGG	8 cut(s)	148, 341, 644, 701, 815, 1698, 2495, 2714
Bme18I	GGWCC	3 cut(s)	1067, 1427, 1640
BmeT110I	CYCGRG	2 cut(s)	2291, 2764
BmgT120I	GGNCC	3 cut(s)	1067, 1427, 1640
BmiI	GGNNCC	2 cut(s)	2255, 2751
BmrFI	CCNGG	8 cut(s)	148, 341, 644, 701, 815, 1698, 2495, 2714
BmrI	ACTGGG	2 cut(s)	2583, 2635
BmsI	GCATC	6 cut(s)	294, 369, 656, 2224, 2322, 2726
BmuI	ACTGGG	2 cut(s)	2583, 2635
BoxI	GACNNNNGTC	1 cut(s)	2867
BpiI	GAAGAC	3 cut(s)	869, 1229, 1798
BplI	GAGNNNNNCTC	2 cut(s)	1142, 1174
BpmI	CTGGAG	4 cut(s)	1158, 1226, 1398, 2097
Bpu1102I	GCTNAGC	1 cut(s)	2018
BpuEI	CTTGAG	4 cut(s)	57, 542, 719, 2161
BpuMI	CCSGG	4 cut(s)	148, 341, 644, 2495
BsaHI	GRCGYC	2 cut(s)	2535, 2750
BsaJI	CCNNGG	8 cut(s)	340, 700, 1696, 1984, 2290, 2397, 2421, 2493
BsaWI	WCCGGW	7 cut(s)	203, 266, 424, 571, 859, 1064, 1568
BsaXI	ACNNNNNCTCC	2 cut(s)	2582, 2612
Bsc4I	CCNNNNNNNGG	2 cut(s)	1945, 2398
Bse118I	RCCGGY	2 cut(s)	1842, 2584
Bse1I	ACTGG	7 cut(s)	100, 252, 814, 853, 1168, 2578, 2630
Bse21I	CCTNAGG	1 cut(s)	789
Bse3DI	GCAATG	2 cut(s)	1070, 2724
BseAI	TCCGGA	3 cut(s)	424, 571, 859
BseBI	CCWGG	4 cut(s)	701, 815, 1698, 2714
BseDI	CCNNGG	8 cut(s)	340, 700, 1696, 1984, 2290, 2397, 2421, 2493
BseGI	GGATG	7 cut(s)	81, 175, 1575, 2035, 2239, 2370, 2510
BseLI	CCNNNNNNNGG	2 cut(s)	1945, 2398
BseMI	GCAATG	2 cut(s)	1070, 2724
BseMII	CTCAG	9 cut(s)	96, 317, 424, 780, 950, 1173, 1420, 2009, 2106
BseNI	ACTGG	7 cut(s)	100, 252, 814, 853, 1168, 2578, 2630
BseRI	GAGGAG	4 cut(s)	806, 1802, 2454, 2717
BseSI	GKGCMC	1 cut(s)	1020
BseX3I	CGGCCG	1 cut(s)	717
BseXI	GCAGC	5 cut(s)	577, 910, 2034, 2745, 2775
BseYI	CCCAGC	2 cut(s)	2128, 2471
Bsh1285I	CGRYCG	1 cut(s)	720
BshFI	GGCC	5 cut(s)	719, 813, 1701, 1983, 2269
BshNI	GGYRCC	1 cut(s)	2749
BsiEI	CGRYCG	1 cut(s)	720
BsiHKAI	GWGCWC	3 cut(s)	390, 759, 1825
BsiHKCI	CYCGRG	2 cut(s)	2291, 2764
BslFI	GGGAC	2 cut(s)	2011, 2488
BslI	CCNNNNNNNGG	2 cut(s)	1945, 2398
BsmAI	GTCTC	6 cut(s)	75, 1176, 1378, 1592, 2117, 2598
BsmBI	CGTCTC	2 cut(s)	75, 2117
BsmFI	GGGAC	2 cut(s)	2011, 2488
BsmI	GAATGC	1 cut(s)	1169
BsnI	GGCC	5 cut(s)	719, 813, 1701, 1983, 2269
BsoBI	CYCGRG	2 cut(s)	2291, 2764
Bsp1286I	GDGCHC	5 cut(s)	390, 759, 1020, 1214, 1825
Bsp13I	TCCGGA	3 cut(s)	424, 571, 859
Bsp1407I	TGTACA	1 cut(s)	2004
Bsp1720I	GCTNAGC	1 cut(s)	2018
Bsp19I	CCATGG	1 cut(s)	1984
BspACI	CCGC	5 cut(s)	690, 705, 1668, 1942, 2025
BspANI	GGCC	5 cut(s)	719, 813, 1701, 1983, 2269
BspCNI	CTCAG	9 cut(s)	97, 316, 423, 781, 951, 1172, 1419, 2010, 2107
BspEI	TCCGGA	3 cut(s)	424, 571, 859
BspLI	GGNNCC	2 cut(s)	2255, 2751
BspMAI	CTGCAG	4 cut(s)	283, 900, 1225, 1372
BspMI	ACCTGC	4 cut(s)	221, 306, 1228, 1375
BspPI	GGATC	5 cut(s)	634, 1999, 2248, 2261, 2704
BspQI	GCTCTTC	4 cut(s)	378, 1300, 1849, 2244
BspT107I	GGYRCC	1 cut(s)	2749
BsrBI	CCGCTC	1 cut(s)	690
BsrDI	GCAATG	2 cut(s)	1070, 2724
BsrFI	RCCGGY	2 cut(s)	1842, 2584
BsrGI	TGTACA	1 cut(s)	2004
BsrI	ACTGG	7 cut(s)	100, 252, 814, 853, 1168, 2578, 2630
BssAI	RCCGGY	2 cut(s)	1842, 2584
BssECI	CCNNGG	8 cut(s)	340, 700, 1696, 1984, 2290, 2397, 2421, 2493
BssNI	GRCGYC	2 cut(s)	2535, 2750
BssT1I	CCWWGG	3 cut(s)	1984, 2397, 2421
Bst2UI	CCWGG	4 cut(s)	701, 815, 1698, 2714
Bst4CI	ACNGT	6 cut(s)	750, 914, 1795, 2002, 2464, 2543
Bst6I	CTCTTC	7 cut(s)	378, 1179, 1300, 1504, 1849, 2039, 2244
BstACI	GRCGYC	2 cut(s)	2535, 2750
BstAUI	TGTACA	1 cut(s)	2004
BstC8I	GCNNGC	2 cut(s)	283, 965
BstDSI	CCRYGG	1 cut(s)	1984
BstEII	GGTNACC	1 cut(s)	2222
BstF5I	GGATG	7 cut(s)	81, 175, 1575, 2035, 2239, 2370, 2510
BstH2I	RGCGCY	3 cut(s)	939, 1843, 2753
BstHHI	GCGC	5 cut(s)	938, 1842, 2302, 2752, 2763
BstMAI	GTCTC	6 cut(s)	75, 1176, 1378, 1592, 2117, 2598
BstMCI	CGRYCG	1 cut(s)	720
BstMWI	GCNNNNNNNGC	6 cut(s)	307, 1689, 1698, 2187, 2677, 2758
BstNI	CCWGG	4 cut(s)	701, 815, 1698, 2714
BstNSI	RCATGY	3 cut(s)	466, 832, 967
BstPAI	GACNNNNGTC	1 cut(s)	2867
BstPI	GGTNACC	1 cut(s)	2222
BstSCI	CCNGG	8 cut(s)	146, 339, 642, 699, 813, 1696, 2493, 2712
BstSFI	CTRYAG	5 cut(s)	279, 896, 1221, 1368, 2539
BstSLI	GKGCMC	1 cut(s)	1020
BstV1I	GCAGC	5 cut(s)	577, 910, 2034, 2745, 2775
BstV2I	GAAGAC	3 cut(s)	869, 1229, 1798
BstX2I	RGATCY	2 cut(s)	639, 2253
BstXI	CCANNNNNNTGG	1 cut(s)	776
BstYI	RGATCY	2 cut(s)	639, 2253
BstZI	CGGCCG	1 cut(s)	717
Bsu36I	CCTNAGG	1 cut(s)	789
BsuRI	GGCC	5 cut(s)	719, 813, 1701, 1983, 2269
BtgI	CCRYGG	1 cut(s)	1984
BtgZI	GCGATG	1 cut(s)	925
BtsCI	GGATG	7 cut(s)	81, 175, 1575, 2035, 2239, 2370, 2510
BtsI	GCAGTG	1 cut(s)	2046
BtsIMutI	CAGTG	6 cut(s)	507, 807, 956, 2007, 2046, 2460
BveI	ACCTGC	4 cut(s)	221, 306, 1228, 1375
Cac8I	GCNNGC	2 cut(s)	283, 965
CaiI	CAGNNNCTG	2 cut(s)	1376, 2791
CfoI	GCGC	5 cut(s)	938, 1842, 2302, 2752, 2763
Cfr10I	RCCGGY	2 cut(s)	1842, 2584
Cfr13I	GGNCC	3 cut(s)	1067, 1427, 1640
CseI	GACGC	2 cut(s)	427, 2134
Csp6I	GTAC	6 cut(s)	87, 406, 469, 527, 2005, 2164
CspCI	CAANNNNNGTGG	4 cut(s)	2449, 2484, 2622, 2657
CviQI	GTAC	6 cut(s)	87, 406, 469, 527, 2005, 2164
DinI	GGCGCC	1 cut(s)	2751
DrdI	GACNNNNNNGTC	2 cut(s)	833, 1233
DseDI	GACNNNNNNGTC	2 cut(s)	833, 1233
EaeI	YGGCCR	1 cut(s)	717
EagI	CGGCCG	1 cut(s)	717
Eam1104I	CTCTTC	7 cut(s)	378, 1179, 1300, 1504, 1849, 2039, 2244
EarI	CTCTTC	7 cut(s)	378, 1179, 1300, 1504, 1849, 2039, 2244
Ecl136II	GAGCTC	3 cut(s)	388, 757, 1823
EclXI	CGGCCG	1 cut(s)	717
Eco130I	CCWWGG	3 cut(s)	1984, 2397, 2421
Eco147I	AGGCCT	1 cut(s)	1701
Eco24I	GRGCYC	4 cut(s)	390, 759, 1214, 1825
Eco32I	GATATC	1 cut(s)	2356
Eco47I	GGWCC	3 cut(s)	1067, 1427, 1640
Eco52I	CGGCCG	1 cut(s)	717
Eco53kI	GAGCTC	3 cut(s)	388, 757, 1823
Eco57I	CTGAAG	1 cut(s)	470
Eco81I	CCTNAGG	1 cut(s)	789
Eco88I	CYCGRG	2 cut(s)	2291, 2764
Eco91I	GGTNACC	1 cut(s)	2222
EcoICRI	GAGCTC	3 cut(s)	388, 757, 1823
EcoO65I	GGTNACC	1 cut(s)	2222
EcoRII	CCWGG	4 cut(s)	699, 813, 1696, 2712
EcoRV	GATATC	1 cut(s)	2356
EcoT14I	CCWWGG	3 cut(s)	1984, 2397, 2421
EcoT22I	ATGCAT	1 cut(s)	2506
EcoT38I	GRGCYC	4 cut(s)	390, 759, 1214, 1825
EgeI	GGCGCC	1 cut(s)	2751
EheI	GGCGCC	1 cut(s)	2751
ErhI	CCWWGG	3 cut(s)	1984, 2397, 2421
Esp3I	CGTCTC	2 cut(s)	75, 2117
FaqI	GGGAC	2 cut(s)	2011, 2488
FauI	CCCGC	2 cut(s)	1661, 2018
FauNDI	CATATG	2 cut(s)	1742, 1903
FblI	GTMKAC	2 cut(s)	2363, 2538
Fnu4HI	GCNGC	6 cut(s)	566, 691, 899, 2023, 2759, 2789
FokI	GGATG	7 cut(s)	68, 182, 1562, 2042, 2246, 2357, 2497
FriOI	GRGCYC	4 cut(s)	390, 759, 1214, 1825
Fsp4HI	GCNGC	6 cut(s)	566, 691, 899, 2023, 2759, 2789
FspBI	CTAG	4 cut(s)	96, 1311, 1391, 2261
FspI	TGCGCA	1 cut(s)	2301
GlaI	GCGC	5 cut(s)	937, 1841, 2301, 2751, 2762
GluI	GCNGC	6 cut(s)	566, 691, 899, 2023, 2759, 2789
GsaI	CCCAGC	2 cut(s)	2132, 2475
GsuI	CTGGAG	4 cut(s)	1158, 1226, 1398, 2097
HaeII	RGCGCY	3 cut(s)	939, 1843, 2753
HaeIII	GGCC	5 cut(s)	719, 813, 1701, 1983, 2269
HgaI	GACGC	2 cut(s)	427, 2134
HhaI	GCGC	5 cut(s)	938, 1842, 2302, 2752, 2763
Hin1I	GRCGYC	2 cut(s)	2535, 2750
Hin6I	GCGC	5 cut(s)	936, 1840, 2300, 2750, 2761
HinP1I	GCGC	5 cut(s)	936, 1840, 2300, 2750, 2761
HindIII	AAGCTT	4 cut(s)	1460, 1631, 1681, 1930
HinfI	GANTC	9 cut(s)	856, 863, 947, 1511, 1585, 2072, 2450, 2690, 2819
HphI	GGTGA	9 cut(s)	202, 355, 356, 426, 505, 719, 935, 1017, 2270
Hpy166II	GTNNAC	7 cut(s)	355, 1748, 2005, 2094, 2364, 2539, 2644
Hpy8I	GTNNAC	7 cut(s)	355, 1748, 2005, 2094, 2364, 2539, 2644
HpyCH4III	ACNGT	6 cut(s)	750, 914, 1795, 2002, 2464, 2543
HpyCH4IV	ACGT	3 cut(s)	328, 620, 2535
HpyF10VI	GCNNNNNNNGC	6 cut(s)	307, 1689, 1698, 2187, 2677, 2758
HpySE526I	ACGT	3 cut(s)	328, 620, 2535
Hsp92I	GRCGYC	2 cut(s)	2535, 2750
HspAI	GCGC	5 cut(s)	936, 1840, 2300, 2750, 2761
KasI	GGCGCC	1 cut(s)	2749
Kpn2I	TCCGGA	3 cut(s)	424, 571, 859
LguI	GCTCTTC	4 cut(s)	378, 1300, 1849, 2244
LmnI	GCTCC	7 cut(s)	754, 793, 891, 1379, 1820, 2563, 2657
Lsp1109I	GCAGC	5 cut(s)	577, 910, 2034, 2745, 2775
LweI	GCATC	6 cut(s)	294, 369, 656, 2224, 2322, 2726
MaeI	CTAG	4 cut(s)	96, 1311, 1391, 2261
MaeII	ACGT	3 cut(s)	328, 620, 2535
MbiI	CCGCTC	1 cut(s)	690
MfeI	CAATTG	3 cut(s)	651, 1299, 1443
MflI	RGATCY	2 cut(s)	639, 2253
MhlI	GDGCHC	5 cut(s)	390, 759, 1020, 1214, 1825
Mly113I	GGCGCC	1 cut(s)	2750
MlyI	GAGTC	3 cut(s)	941, 1594, 2684
MmeI	TCCRAC	4 cut(s)	732, 757, 1578, 2231
Mph1103I	ATGCAT	1 cut(s)	2506
MroI	TCCGGA	3 cut(s)	424, 571, 859
MroXI	GAANNNNTTC	1 cut(s)	2449
MseI	TTAA	6 cut(s)	63, 1454, 1464, 1710, 1758, 1772
MslI	CAYNNNNRTG	1 cut(s)	500
MspA1I	CMGCKG	3 cut(s)	2025, 2089, 2788
MspR9I	CCNGG	8 cut(s)	148, 341, 644, 701, 815, 1698, 2495, 2714
MunI	CAATTG	3 cut(s)	651, 1299, 1443
Mva1269I	GAATGC	1 cut(s)	1169
MvaI	CCWGG	4 cut(s)	701, 815, 1698, 2714
MwoI	GCNNNNNNNGC	6 cut(s)	307, 1689, 1698, 2187, 2677, 2758
NarI	GGCGCC	1 cut(s)	2750
NciI	CCSGG	4 cut(s)	148, 341, 644, 2495
NcoI	CCATGG	1 cut(s)	1984
NdeI	CATATG	2 cut(s)	1742, 1903
NlaIV	GGNNCC	2 cut(s)	2255, 2751
NmeAIII	GCCGAG	2 cut(s)	565, 745
NmuCI	GTSAC	6 cut(s)	208, 258, 344, 2276, 2531, 2860
NsbI	TGCGCA	1 cut(s)	2301
NsiI	ATGCAT	1 cut(s)	2506
NspI	RCATGY	3 cut(s)	466, 832, 967
PaeI	GCATGC	1 cut(s)	967
PaqCI	CACCTGC	2 cut(s)	221, 1375
PceI	AGGCCT	1 cut(s)	1701
PciI	ACATGT	2 cut(s)	462, 828
PciSI	GCTCTTC	4 cut(s)	378, 1300, 1849, 2244
PcsI	WCGNNNNNNNCGW	1 cut(s)	2762
PctI	GAATGC	1 cut(s)	1169
PdmI	GAANNNNTTC	1 cut(s)	2449
PfeI	GAWTC	6 cut(s)	856, 863, 1511, 2072, 2450, 2819
PkrI	GCNGC	6 cut(s)	567, 692, 900, 2024, 2760, 2790
PleI	GAGTC	3 cut(s)	941, 1593, 2684
PluTI	GGCGCC	1 cut(s)	2753
PpsI	GAGTC	3 cut(s)	941, 1593, 2684
PscI	ACATGT	2 cut(s)	462, 828
PshAI	GACNNNNGTC	1 cut(s)	2867
PsiI	TTATAA	2 cut(s)	12, 47
Psp124BI	GAGCTC	3 cut(s)	390, 759, 1825
Psp6I	CCWGG	4 cut(s)	699, 813, 1696, 2712
PspEI	GGTNACC	1 cut(s)	2222
PspFI	CCCAGC	2 cut(s)	2128, 2471
PspGI	CCWGG	4 cut(s)	699, 813, 1696, 2712
PspN4I	GGNNCC	2 cut(s)	2255, 2751
PspPI	GGNCC	3 cut(s)	1067, 1427, 1640
PsrI	GAACNNNNNNTAC	4 cut(s)	461, 493, 2606, 2638
PstI	CTGCAG	4 cut(s)	283, 900, 1225, 1372
PstNI	CAGNNNCTG	2 cut(s)	1376, 2791
PsuI	RGATCY	2 cut(s)	639, 2253
PvuII	CAGCTG	2 cut(s)	2089, 2788
RsaI	GTAC	6 cut(s)	88, 407, 470, 528, 2006, 2165
RsaNI	GTAC	6 cut(s)	87, 406, 469, 527, 2005, 2164
RseI	CAYNNNNRTG	1 cut(s)	500
SacI	GAGCTC	3 cut(s)	390, 759, 1825
SapI	GCTCTTC	4 cut(s)	378, 1300, 1849, 2244
SaqAI	TTAA	6 cut(s)	63, 1454, 1464, 1710, 1758, 1772
SatI	GCNGC	6 cut(s)	566, 691, 899, 2023, 2759, 2789
Sau96I	GGNCC	3 cut(s)	1067, 1427, 1640
SbfI	CCTGCAGG	2 cut(s)	283, 1225
SchI	GAGTC	3 cut(s)	941, 1594, 2684
ScrFI	CCNGG	8 cut(s)	148, 341, 644, 701, 815, 1698, 2495, 2714
SdaI	CCTGCAGG	2 cut(s)	283, 1225
SduI	GDGCHC	5 cut(s)	390, 759, 1020, 1214, 1825
SfaNI	GCATC	6 cut(s)	294, 369, 656, 2224, 2322, 2726
SfcI	CTRYAG	5 cut(s)	279, 896, 1221, 1368, 2539
SfoI	GGCGCC	1 cut(s)	2751
SinI	GGWCC	3 cut(s)	1067, 1427, 1640
SmiMI	CAYNNNNRTG	1 cut(s)	500
SmlI	CTYRAG	4 cut(s)	72, 521, 734, 2176
SmoI	CTYRAG	4 cut(s)	72, 521, 734, 2176
SphI	GCATGC	1 cut(s)	967
Sse8387I	CCTGCAGG	2 cut(s)	283, 1225
SseBI	AGGCCT	1 cut(s)	1701
SsiI	CCGC	5 cut(s)	690, 705, 1668, 1942, 2025
SspDI	GGCGCC	1 cut(s)	2749
SspMI	CTAG	4 cut(s)	96, 1311, 1391, 2261
SstI	GAGCTC	3 cut(s)	390, 759, 1825
StuI	AGGCCT	1 cut(s)	1701
StyD4I	CCNGG	8 cut(s)	146, 339, 642, 699, 813, 1696, 2493, 2712
StyI	CCWWGG	3 cut(s)	1984, 2397, 2421
TaaI	ACNGT	6 cut(s)	750, 914, 1795, 2002, 2464, 2543
TaiI	ACGT	3 cut(s)	331, 623, 2538
TatI	WGTACW	2 cut(s)	2004, 2163
TauI	GCSGC	1 cut(s)	693
TfiI	GAWTC	6 cut(s)	856, 863, 1511, 2072, 2450, 2819
Tru1I	TTAA	6 cut(s)	63, 1454, 1464, 1710, 1758, 1772
Tru9I	TTAA	6 cut(s)	63, 1454, 1464, 1710, 1758, 1772
TscAI	CASTG	6 cut(s)	507, 814, 963, 2007, 2046, 2467
TseFI	GTSAC	6 cut(s)	208, 258, 344, 2276, 2531, 2860
TseI	GCWGC	5 cut(s)	565, 898, 2022, 2758, 2788
Tsp45I	GTSAC	6 cut(s)	208, 258, 344, 2276, 2531, 2860
TspDTI	ATGAA	7 cut(s)	455, 469, 555, 1707, 2499, 2786, 2811
TspGWI	ACGGA	5 cut(s)	57, 74, 308, 1094, 2678
TspRI	CASTG	6 cut(s)	507, 814, 963, 2007, 2046, 2467
VpaK11BI	GGWCC	3 cut(s)	1067, 1427, 1640
XapI	RAATTY	4 cut(s)	711, 739, 999, 1774
XbaI	TCTAGA	1 cut(s)	2260
XceI	RCATGY	3 cut(s)	466, 832, 967
XmiI	GTMKAC	2 cut(s)	2363, 2538
XmnI	GAANNNNTTC	1 cut(s)	2449
XspI	CTAG	4 cut(s)	96, 1311, 1391, 2261
ZraI	GACGTC	1 cut(s)	2536
Zsp2I	ATGCAT	1 cut(s)	2506

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.