Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0011774

Physical Location & Seq

Genomic Coordinates Reverse (-)

58951241 .. 58957563

Gene Structure 6323 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG418800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2511 bp

ATGGCCTCTCACATAACCTACTTCCTTTCTGTGTTACTTATAATATTCTTGGTTTTGTATCCTTCTGAAGCCTTGATCACTAGTAGTAACCAGTCTCAGTTTTTTGTGCAAATAATAAAGTCTCTTTCACCTAATTCGGGTAGCTCTTTGTCAGATTGGGATGTCAAAGGAGGAAAACCTTACTGTAACTTCTCTGGGGTTAGCTGCAATGATGATGGCTACGTTGTCCGGATTGATATTTCCGGTCGATCACTCTCAGGTCACTTCCCAGCAGACATATGCTCCTACCTGCCTCAGTTGCGCATTCTCCGTCTTGGTCACAACAACCTCCAGGGTGACTTTGTTGATAGCATCACCAACTGCTCATTCTTGGAAGAGCTCAGCATGGATCATTTGTATCTCAGTGGAACTCTACCAGATTTTTCACCCTTGAAGAATCTAAAGATACTTGACTTGTCTTACAACAAGTTCAAAGGCAAGTTCCCCATGTCAGTGTTTAATCTTACCAATCTTGAGGTGCTCAATTTCAATGAGAATGGAGATTTCAACTTATGGCAGCTGCCGGAGAATATACACACACTGACAAAGCTCAAATCCATGGTCTTAACAACATGCATGGTACAAGGGAAGATCCCAACATCAATAGGAAACATGACTTCCCTTGTTGATCTTGAACTGAGTGGAAATTACCTGGTGGGTCAAATTCCAACAGAGATTGGATTGCTCAAGAATTTGAAGCAATTGGAGCTGTACTACAACCAACTCACTGGTAACATACCTGAGGAGCTCGGAAATCTCACAGACCTCACTGATATGGACATGTCAGTCAATAAGTTGACTGGGAAGATTCCAGAGTCAATCTGTCGGCTTCCCCGGCTTCAAGTCCTGCAGCTTTACAACAACAGCCTTTCAGGAGAGATCCCAACTGTGATTGCAGACTCAAAAACACTGAGCATGCTGTCACTTTATGACAATTTCCTGACTGGAGAAGTTCCAAGAAATCTGGGGAAATCATCAGCTATAGTTGTCCTCGACTTGTCAGAGAACCAACTTTCTGGTCCATTACCAACAGAGGTTTGCAGGGGAGGTAAGTTGCTCTACTTTCTTATTCTGGAAAACCAGTTTTCTGGAGAAATACCAGACAGCTATGCAGGATGTGAATCTCTTCTTCGATTTCGGCTTAGCTTTAATCGTTTGGAGGGCTCGATACCTGCAGGACTTCTTAATCTTCCCCATGTTTCCATCTTTGATCTGGCTTACAACAATCTGAGTGGTCAGATTGCTGATACAATTGGGAGAGCCAGAAATTTGTCTGAACTTTTTATCCAAAACAACAGCATTTCAGGTGTTCTGCCCCCTGGAATCTCTGGAGCAATAAGTCTAGTGAAGATTGATCTCAGTAATAATCTTATTTCTGGTCCAATTCCTTCAGAAATTGGAAAATTAAAGAAGCTGAATTTACTGATGTTGCAAGGCAACAAGCTCACTTCTTCCATTCCTGATTCACTTTCTTTGTTGAAATCACTCAATGTTCTTGATCTCTCCAACAACCTCTTGACAGGAAATATCCCTGATAGTCTTTGTGAATTGTTACCAAACTCTATCAACTTCTCAAACAATAAGCTTTCAGGTCCTATTCCAGTCCATTTGATAGAAGGAGGGCTAATAGAAAGCTTTTCTGGCAACCCTGGCCTTTGTGTGAAAGTCTATGTTAACTCATCTGATCAAAATAGGTTTCCCGTATGCCCACAACACTTCAACAGAAAGAAAATAAATTCCTTTTGGGTAGTAACAGTTTCAGTAGTTATTATTCTTATTGGAGCTATTCTCTTTCTGAAGCGCCGATTTGGAAAAGAAAGAGCAGAAGTGGAACATGAAGAATCTCTATCCTCATCATTCTTCTGCTATGATGTGAAAAGTTTTCATCGAATAAGTTTTGACCATCGTGAGGTCATTGAAGCTATGGTTGACAAGAACATAGTAGGCAATGGAGGATCTGGGACAGTTTACAAGATCGAGTTGAGCAGTGGGGACGTAGTTGCAGTGAAGAGACTATGGAGTAAGAAAACAAAAGAATCTTCAGAAGATGATCAGTTTGTTATACACAAGGAGTTGAAAACTGAGGTAGAGACTCTGGGTAACATAAGGCATAAGAACATTGTAAAATTGTTTTGCTACTTCTCAAGTTTGGATTGCAACCTGTTGGTTTATGAGTATATGCCGAATGGAAACCTTTGGGATGCTCTTCACAAAGGTTGGATCCATTTGGAGTGGCCTACTCGTCACCAGATCGCGCTAGGGATTGCACAGGGCTTGTCATATCTCCACCATGATCTGATGCCTCCAATTATTCACAGAGATATTAAGTCCACCAACATACTGCTTGATGTGAATTACCAACCCAAAGTTGCAGATTTTGGCATAGCAAAGGTTTTACAAGCAAGAGGAGGGAAAGATTCCACAACCACTGTTATCGCTGGGACATACGGTTACTTAGCCCCAGGTACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001653 GO:0003002 GO:0003674 GO:0005488 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009962 GO:0009987 GO:0009991 GO:0010015 GO:0010051 GO:0010101 GO:0010102 GO:0010311 GO:0015698 GO:0015706 GO:0017046 GO:0019222 GO:0022622 GO:0023052 GO:0031323 GO:0031326 GO:0031537 GO:0031540 GO:0031667 GO:0032501 GO:0032502 GO:0033218 GO:0038023 GO:0042277 GO:0042562 GO:0042594 GO:0043455 GO:0044087 GO:0048364 GO:0048518 GO:0048527 GO:0048528 GO:0048580 GO:0048582 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051716 GO:0060089 GO:0065007 GO:0071705 GO:0080090 GO:0090548 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1900376 GO:1901141 GO:1901333 GO:1902025 GO:1903338 GO:1905392 GO:1905393 GO:2000023 GO:2000026 GO:2000069 GO:2000280 GO:2000652 GO:2000762

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

836

Amino Acids

92.54

Weight (kDa)

6.12

Isoelectric Point (pI)

35.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	29 - 70	4.4e-06	Leucine rich repeat N-terminal domain
LRR_14	PF23598	138 - 253	8.7e-11	Leucine-rich repeat region
LRR_14	PF23598	238 - 448	6e-08	Leucine-rich repeat region
Pkinase	PF00069	658 - 834	1.5e-33	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	659 - 815	1.3e-24	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0011774)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49660
fragaria_vesca	FvH4_5g33580 FvH4_5g33580
malus_domestica	MD08G1210400.v1.1 MD12G1134800.v1.1 MD15G1394300.v1.1
prunus_persica	Prupe.1G542400_v2.0.a1
pyrus_communis	pycom08g18140 pycom15g35440 pycom15g35450
rosa_chinensis	RchiOBHm_Chr7g0232421
rosa_laevigata	RLG00000001343
rosa_multiflora	Rmu_sc0003113.1_g000026
rosa_roxburghii	Rroxscaffold_3G00228340
rosa_samantha	Rh7AG427300 Rh7BG402200 Rh7CG446800 Rh7DG418800
rosa_wichuraiana	Rw7G035460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	41
AasI	GACNNNNNNGTC	1 cut(s)	824
Acc16I	TGCGCA	1 cut(s)	302
Acc36I	ACCTGC	2 cut(s)	297, 1219
AccII	CGCG	1 cut(s)	2294
AccIII	TCCGGA	1 cut(s)	228
AclWI	GGATC	6 cut(s)	396, 625, 913, 2002, 2254, 2267
AcsI	RAATTY	5 cut(s)	702, 730, 1306, 1456, 1774
AcuI	CTGAAG	4 cut(s)	87, 1413, 1856, 2064
AfaI	GTAC	3 cut(s)	621, 752, 2506
AfiI	CCNNNNNNNGG	2 cut(s)	137, 1948
AflIII	ACRYGT	1 cut(s)	819
AhlI	ACTAGT	1 cut(s)	80
AjnI	CCWGG	5 cut(s)	330, 690, 1357, 1687, 2500
AjuI	GAANNNNNNNTTGG	2 cut(s)	350, 382
AloI	GAACNNNNNNTCC	2 cut(s)	1308, 1340
Alw21I	GWGCWC	3 cut(s)	381, 522, 789
Alw26I	GTCTC	4 cut(s)	99, 126, 2044, 2123
AlwI	GGATC	6 cut(s)	396, 625, 913, 2002, 2254, 2267
Aor13HI	TCCGGA	1 cut(s)	228
AoxI	GGCC	3 cut(s)	3, 1690, 2273
ApeKI	GCWGC	4 cut(s)	204, 556, 559, 889
ApoI	RAATTY	5 cut(s)	702, 730, 1306, 1456, 1774
ArsI	GACNNNNNNTTYG	2 cut(s)	585, 617
Asp700I	GAANNNNTTC	1 cut(s)	1164
AspLEI	GCGC	3 cut(s)	303, 1842, 2296
AspS9I	GGNCC	3 cut(s)	1058, 1418, 1631
AsuC2I	CCSGG	1 cut(s)	874
AsuHPI	GGTGA	5 cut(s)	120, 346, 347, 417, 2276
AvaII	GGWCC	3 cut(s)	1058, 1418, 1631
AxyI	CCTNAGG	1 cut(s)	780
BamHI	GGATCC	1 cut(s)	2259
BanII	GRGCYC	3 cut(s)	381, 789, 1205
Bbv12I	GWGCWC	3 cut(s)	381, 522, 789
BbvI	GCAGC	4 cut(s)	191, 546, 568, 901
BccI	CCATC	3 cut(s)	209, 1250, 1950
BciT130I	CCWGG	5 cut(s)	332, 692, 1359, 1689, 2502
BciVI	GTATCC	1 cut(s)	69
BclI	TGATCA	3 cut(s)	75, 1723, 2089
BcnI	CCSGG	1 cut(s)	874
BcoDI	GTCTC	4 cut(s)	99, 126, 2044, 2123
BcuI	ACTAGT	1 cut(s)	80
BfaI	CTAG	3 cut(s)	81, 1382, 2297
BfmI	CTRYAG	3 cut(s)	887, 1020, 1212
BfoI	RGCGCY	1 cut(s)	1843
BfuAI	ACCTGC	2 cut(s)	297, 1219
BfuI	GTATCC	1 cut(s)	69
BisI	GCNGC	4 cut(s)	205, 557, 560, 890
BlpI	GCTNAGC	2 cut(s)	380, 1181
BlsI	GCNGC	4 cut(s)	206, 558, 561, 891
Bme1390I	CCNGG	6 cut(s)	332, 692, 874, 1359, 1689, 2502
Bme18I	GGWCC	3 cut(s)	1058, 1418, 1631
BmgT120I	GGNCC	3 cut(s)	1058, 1418, 1631
BmiI	GGNNCC	1 cut(s)	2261
BmrFI	CCNGG	6 cut(s)	332, 692, 874, 1359, 1689, 2502
BmrI	ACTGGG	1 cut(s)	849
BmsI	GCATC	3 cut(s)	360, 2230, 2328
BmuI	ACTGGG	1 cut(s)	849
BplI	GAGNNNNNCTC	2 cut(s)	1812, 1844
BpmI	CTGGAG	4 cut(s)	314, 1005, 1149, 1389
Bpu1102I	GCTNAGC	2 cut(s)	380, 1181
BpuEI	CTTGAG	3 cut(s)	533, 710, 2167
BpuMI	CCSGG	1 cut(s)	874
BsaBI	GATNNNNATC	1 cut(s)	1159
BsaJI	CCNNGG	6 cut(s)	331, 597, 872, 1357, 1687, 2500
BsaWI	WCCGGW	2 cut(s)	228, 242
BsaXI	ACNNNNNCTCC	2 cut(s)	266, 296
Bsc4I	CCNNNNNNNGG	2 cut(s)	137, 1948
Bse1I	ACTGG	6 cut(s)	91, 772, 844, 988, 1120, 1640
Bse21I	CCTNAGG	1 cut(s)	780
Bse3DI	GCAATG	2 cut(s)	214, 1993
Bse8I	GATNNNNATC	1 cut(s)	1159
BseAI	TCCGGA	1 cut(s)	228
BseBI	CCWGG	5 cut(s)	332, 692, 1359, 1689, 2502
BseDI	CCNNGG	6 cut(s)	331, 597, 872, 1357, 1687, 2500
BseGI	GGATG	3 cut(s)	166, 1160, 2245
BseJI	GATNNNNATC	1 cut(s)	1159
BseLI	CCNNNNNNNGG	2 cut(s)	137, 1948
BseMI	GCAATG	2 cut(s)	214, 1993
BseNI	ACTGG	6 cut(s)	91, 772, 844, 988, 1120, 1640
BseRI	GAGGAG	2 cut(s)	797, 2460
BseXI	GCAGC	4 cut(s)	191, 546, 568, 901
BseYI	CCCAGC	2 cut(s)	268, 2477
Bsh1236I	CGCG	1 cut(s)	2294
Bsh1285I	CGRYCG	1 cut(s)	247
BshFI	GGCC	3 cut(s)	5, 1692, 2275
BsiEI	CGRYCG	1 cut(s)	247
BsiHKAI	GWGCWC	3 cut(s)	381, 522, 789
BsiSI	CCGG	4 cut(s)	229, 243, 563, 874
BslFI	GGGAC	3 cut(s)	2014, 2045, 2494
BslI	CCNNNNNNNGG	2 cut(s)	137, 1948
BsmAI	GTCTC	4 cut(s)	99, 126, 2044, 2123
BsmFI	GGGAC	3 cut(s)	2014, 2045, 2494
BsmI	GAATGC	1 cut(s)	303
BsnI	GGCC	3 cut(s)	5, 1692, 2275
Bsp1286I	GDGCHC	4 cut(s)	381, 522, 789, 1205
Bsp13I	TCCGGA	1 cut(s)	228
Bsp1720I	GCTNAGC	2 cut(s)	380, 1181
Bsp19I	CCATGG	1 cut(s)	597
BspANI	GGCC	3 cut(s)	5, 1692, 2275
BspEI	TCCGGA	1 cut(s)	228
BspFNI	CGCG	1 cut(s)	2294
BspLI	GGNNCC	1 cut(s)	2261
BspMAI	CTGCAG	2 cut(s)	891, 1216
BspMI	ACCTGC	2 cut(s)	297, 1219
BspPI	GGATC	6 cut(s)	396, 625, 913, 2002, 2254, 2267
BspQI	GCTCTTC	2 cut(s)	369, 2250
BsrDI	GCAATG	2 cut(s)	214, 1993
BsrI	ACTGG	6 cut(s)	91, 772, 844, 988, 1120, 1640
BssECI	CCNNGG	6 cut(s)	331, 597, 872, 1357, 1687, 2500
BssT1I	CCWWGG	1 cut(s)	597
Bst2UI	CCWGG	5 cut(s)	332, 692, 1359, 1689, 2502
Bst4CI	ACNGT	6 cut(s)	185, 928, 1795, 2005, 2470, 2489
Bst6I	CTCTTC	4 cut(s)	369, 1170, 2042, 2250
BstC8I	GCNNGC	1 cut(s)	956
BstDSI	CCRYGG	1 cut(s)	597
BstF5I	GGATG	3 cut(s)	166, 1160, 2245
BstFNI	CGCG	1 cut(s)	2294
BstH2I	RGCGCY	1 cut(s)	1843
BstHHI	GCGC	3 cut(s)	303, 1842, 2296
BstMAI	GTCTC	4 cut(s)	99, 126, 2044, 2123
BstMCI	CGRYCG	1 cut(s)	247
BstMWI	GCNNNNNNNGC	5 cut(s)	298, 745, 874, 1680, 1689
BstNI	CCWGG	5 cut(s)	332, 692, 1359, 1689, 2502
BstNSI	RCATGY	3 cut(s)	615, 823, 958
BstSCI	CCNGG	6 cut(s)	330, 690, 872, 1357, 1687, 2500
BstSFI	CTRYAG	3 cut(s)	887, 1020, 1212
BstUI	CGCG	1 cut(s)	2294
BstV1I	GCAGC	4 cut(s)	191, 546, 568, 901
BstX2I	RGATCY	4 cut(s)	630, 918, 1994, 2259
BstXI	CCANNNNNNTGG	3 cut(s)	767, 1055, 1127
BstYI	RGATCY	4 cut(s)	630, 918, 1994, 2259
Bsu36I	CCTNAGG	1 cut(s)	780
BsuI	GTATCC	1 cut(s)	69
BsuRI	GGCC	3 cut(s)	5, 1692, 2275
BtgI	CCRYGG	1 cut(s)	597
BtsCI	GGATG	3 cut(s)	166, 1160, 2245
BtsI	GCAGTG	2 cut(s)	2032, 2049
BtsIMutI	CAGTG	9 cut(s)	409, 498, 578, 765, 807, 947, 2032, 2049, 2466
BveI	ACCTGC	2 cut(s)	297, 1219
Cac8I	GCNNGC	1 cut(s)	956
CfoI	GCGC	3 cut(s)	303, 1842, 2296
Cfr13I	GGNCC	3 cut(s)	1058, 1418, 1631
CsiI	ACCWGGT	1 cut(s)	690
Csp6I	GTAC	3 cut(s)	620, 751, 2505
CspCI	CAANNNNNGTGG	2 cut(s)	1252, 1287
CviQI	GTAC	3 cut(s)	620, 751, 2505
DrdI	GACNNNNNNGTC	1 cut(s)	824
DseDI	GACNNNNNNGTC	1 cut(s)	824
Eam1104I	CTCTTC	4 cut(s)	369, 1170, 2042, 2250
EarI	CTCTTC	4 cut(s)	369, 1170, 2042, 2250
Ecl136II	GAGCTC	2 cut(s)	379, 787
Eco130I	CCWWGG	1 cut(s)	597
Eco24I	GRGCYC	3 cut(s)	381, 789, 1205
Eco47I	GGWCC	3 cut(s)	1058, 1418, 1631
Eco53kI	GAGCTC	2 cut(s)	379, 787
Eco57I	CTGAAG	4 cut(s)	87, 1413, 1856, 2064
Eco81I	CCTNAGG	1 cut(s)	780
EcoICRI	GAGCTC	2 cut(s)	379, 787
EcoO109I	RGGNCCY	1 cut(s)	1631
EcoRII	CCWGG	5 cut(s)	330, 690, 1357, 1687, 2500
EcoT14I	CCWWGG	1 cut(s)	597
EcoT22I	ATGCAT	1 cut(s)	617
EcoT38I	GRGCYC	3 cut(s)	381, 789, 1205
ErhI	CCWWGG	1 cut(s)	597
FaqI	GGGAC	3 cut(s)	2014, 2045, 2494
FauNDI	CATATG	1 cut(s)	278
FbaI	TGATCA	3 cut(s)	75, 1723, 2089
Fnu4HI	GCNGC	4 cut(s)	205, 557, 560, 890
FokI	GGATG	3 cut(s)	173, 1167, 2252
FriOI	GRGCYC	3 cut(s)	381, 789, 1205
Fsp4HI	GCNGC	4 cut(s)	205, 557, 560, 890
FspBI	CTAG	3 cut(s)	81, 1382, 2297
FspI	TGCGCA	1 cut(s)	302
GlaI	GCGC	3 cut(s)	302, 1841, 2295
GluI	GCNGC	4 cut(s)	205, 557, 560, 890
GsaI	CCCAGC	2 cut(s)	272, 2481
GsuI	CTGGAG	4 cut(s)	314, 1005, 1149, 1389
HaeII	RGCGCY	1 cut(s)	1843
HaeIII	GGCC	3 cut(s)	5, 1692, 2275
HapII	CCGG	4 cut(s)	229, 243, 563, 874
HhaI	GCGC	3 cut(s)	303, 1842, 2296
Hin6I	GCGC	3 cut(s)	301, 1840, 2294
HinP1I	GCGC	3 cut(s)	301, 1840, 2294
HincII	GTYRAC	3 cut(s)	837, 1714, 1969
HindII	GTYRAC	3 cut(s)	837, 1714, 1969
HindIII	AAGCTT	2 cut(s)	1622, 1672
HpaI	GTTAAC	1 cut(s)	1714
HpaII	CCGG	4 cut(s)	229, 243, 563, 874
HphI	GGTGA	5 cut(s)	120, 346, 347, 417, 2276
Hpy166II	GTNNAC	5 cut(s)	837, 1714, 1969, 2008, 2370
Hpy8I	GTNNAC	5 cut(s)	837, 1714, 1969, 2008, 2370
HpyAV	CCTTC	3 cut(s)	72, 1437, 1649
HpyCH4III	ACNGT	6 cut(s)	185, 928, 1795, 2005, 2470, 2489
HpyCH4IV	ACGT	2 cut(s)	222, 2034
HpyF10VI	GCNNNNNNNGC	5 cut(s)	298, 745, 874, 1680, 1689
HpySE526I	ACGT	2 cut(s)	222, 2034
HspAI	GCGC	3 cut(s)	301, 1840, 2294
Kpn2I	TCCGGA	1 cut(s)	228
Ksp22I	TGATCA	3 cut(s)	75, 1723, 2089
KspAI	GTTAAC	1 cut(s)	1714
LguI	GCTCTTC	2 cut(s)	369, 2250
LmnI	GCTCC	5 cut(s)	287, 745, 784, 1370, 1820
Lsp1109I	GCAGC	4 cut(s)	191, 546, 568, 901
LweI	GCATC	3 cut(s)	360, 2230, 2328
MabI	ACCWGGT	1 cut(s)	690
MaeI	CTAG	3 cut(s)	81, 1382, 2297
MaeII	ACGT	2 cut(s)	222, 2034
MfeI	CAATTG	2 cut(s)	740, 1290
MflI	RGATCY	4 cut(s)	630, 918, 1994, 2259
MhlI	GDGCHC	4 cut(s)	381, 522, 789, 1205
MlyI	GAGTC	3 cut(s)	863, 932, 2125
MmeI	TCCRAC	3 cut(s)	731, 1569, 2237
Mph1103I	ATGCAT	1 cut(s)	617
MroI	TCCGGA	1 cut(s)	228
MroXI	GAANNNNTTC	1 cut(s)	1164
MseI	TTAA	8 cut(s)	498, 605, 1188, 1224, 1445, 1713, 2364, 2509
MslI	CAYNNNNRTG	2 cut(s)	491, 812
MspA1I	CMGCKG	1 cut(s)	559
MspI	CCGG	4 cut(s)	229, 243, 563, 874
MspR9I	CCNGG	6 cut(s)	332, 692, 874, 1359, 1689, 2502
MunI	CAATTG	2 cut(s)	740, 1290
Mva1269I	GAATGC	1 cut(s)	303
MvaI	CCWGG	5 cut(s)	332, 692, 1359, 1689, 2502
MvnI	CGCG	1 cut(s)	2294
MwoI	GCNNNNNNNGC	5 cut(s)	298, 745, 874, 1680, 1689
NciI	CCSGG	1 cut(s)	874
NcoI	CCATGG	1 cut(s)	597
NdeI	CATATG	1 cut(s)	278
NlaIV	GGNNCC	1 cut(s)	2261
NmuCI	GTSAC	5 cut(s)	260, 317, 335, 960, 2282
NsbI	TGCGCA	1 cut(s)	302
NsiI	ATGCAT	1 cut(s)	617
NspI	RCATGY	3 cut(s)	615, 823, 958
PaeI	GCATGC	1 cut(s)	958
PciI	ACATGT	1 cut(s)	819
PciSI	GCTCTTC	2 cut(s)	369, 2250
PctI	GAATGC	1 cut(s)	303
PdmI	GAANNNNTTC	1 cut(s)	1164
PfeI	GAWTC	8 cut(s)	436, 847, 1160, 1362, 1502, 1880, 2075, 2456
PkrI	GCNGC	4 cut(s)	206, 558, 561, 891
PleI	GAGTC	3 cut(s)	862, 932, 2125
PpsI	GAGTC	3 cut(s)	862, 932, 2125
PpuMI	RGGWCCY	1 cut(s)	1631
PscI	ACATGT	1 cut(s)	819
PsiI	TTATAA	1 cut(s)	41
Psp124BI	GAGCTC	2 cut(s)	381, 789
Psp5II	RGGWCCY	1 cut(s)	1631
Psp6I	CCWGG	5 cut(s)	330, 690, 1357, 1687, 2500
PspFI	CCCAGC	2 cut(s)	268, 2477
PspGI	CCWGG	5 cut(s)	330, 690, 1357, 1687, 2500
PspN4I	GGNNCC	1 cut(s)	2261
PspPI	GGNCC	3 cut(s)	1058, 1418, 1631
PspPPI	RGGWCCY	1 cut(s)	1631
PstI	CTGCAG	2 cut(s)	891, 1216
PsuI	RGATCY	4 cut(s)	630, 918, 1994, 2259
PvuII	CAGCTG	1 cut(s)	559
RsaI	GTAC	3 cut(s)	621, 752, 2506
RsaNI	GTAC	3 cut(s)	620, 751, 2505
RseI	CAYNNNNRTG	2 cut(s)	491, 812
SacI	GAGCTC	2 cut(s)	381, 789
SapI	GCTCTTC	2 cut(s)	369, 2250
SaqAI	TTAA	8 cut(s)	498, 605, 1188, 1224, 1445, 1713, 2364, 2509
SatI	GCNGC	4 cut(s)	205, 557, 560, 890
Sau96I	GGNCC	3 cut(s)	1058, 1418, 1631
SbfI	CCTGCAGG	1 cut(s)	1216
SchI	GAGTC	3 cut(s)	863, 932, 2125
ScrFI	CCNGG	6 cut(s)	332, 692, 874, 1359, 1689, 2502
SdaI	CCTGCAGG	1 cut(s)	1216
SduI	GDGCHC	4 cut(s)	381, 522, 789, 1205
SexAI	ACCWGGT	1 cut(s)	690
SfaNI	GCATC	3 cut(s)	360, 2230, 2328
SfcI	CTRYAG	3 cut(s)	887, 1020, 1212
SinI	GGWCC	3 cut(s)	1058, 1418, 1631
SmiMI	CAYNNNNRTG	2 cut(s)	491, 812
SmlI	CTYRAG	3 cut(s)	512, 725, 2182
SmoI	CTYRAG	3 cut(s)	512, 725, 2182
SpeI	ACTAGT	1 cut(s)	80
SphI	GCATGC	1 cut(s)	958
Sse8387I	CCTGCAGG	1 cut(s)	1216
SspI	AATATT	1 cut(s)	45
SspMI	CTAG	3 cut(s)	81, 1382, 2297
SstI	GAGCTC	2 cut(s)	381, 789
StyD4I	CCNGG	6 cut(s)	330, 690, 872, 1357, 1687, 2500
StyI	CCWWGG	1 cut(s)	597
TaaI	ACNGT	6 cut(s)	185, 928, 1795, 2005, 2470, 2489
TaiI	ACGT	2 cut(s)	225, 2037
TaqI	TCGA	6 cut(s)	247, 1032, 1171, 1205, 1927, 2016
TatI	WGTACW	1 cut(s)	750
TfiI	GAWTC	8 cut(s)	436, 847, 1160, 1362, 1502, 1880, 2075, 2456
Tru1I	TTAA	8 cut(s)	498, 605, 1188, 1224, 1445, 1713, 2364, 2509
Tru9I	TTAA	8 cut(s)	498, 605, 1188, 1224, 1445, 1713, 2364, 2509
TscAI	CASTG	9 cut(s)	409, 498, 585, 772, 814, 954, 2032, 2049, 2473
TseFI	GTSAC	5 cut(s)	260, 317, 335, 960, 2282
TseI	GCWGC	4 cut(s)	204, 556, 559, 889
Tsp45I	GTSAC	5 cut(s)	260, 317, 335, 960, 2282
TspDTI	ATGAA	2 cut(s)	1890, 1913
TspGWI	ACGGA	1 cut(s)	299
TspRI	CASTG	9 cut(s)	409, 498, 585, 772, 814, 954, 2032, 2049, 2473
VpaK11BI	GGWCC	3 cut(s)	1058, 1418, 1631
XapI	RAATTY	5 cut(s)	702, 730, 1306, 1456, 1774
XceI	RCATGY	3 cut(s)	615, 823, 958
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1249
XmnI	GAANNNNTTC	1 cut(s)	1164
XspI	CTAG	3 cut(s)	81, 1382, 2297
Zsp2I	ATGCAT	1 cut(s)	617

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.