MD08G1211300.v1.1
NAC Family

inactive poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013684
Physical Location & Seq
Forward (+)
27491140 .. 27492456
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1211300.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGGCTTCCTACGCCGCCACCACCCCCAAGAAAATCCTCCAATCGGTCACCGTTAGGGTTCCGAAGGGGATCCTCTCGCCGTCGTCTTCCTCAGCGAGCTCTAAAAACTTCTGCAATCGGACGTCCAATCGGATGCACTACCCCTCCAATTTCAAGCGGAGCGCCGCCCCGGCACGGGTTATGTTCTTCCAGAACGAGTCCTGGGACGACTTGCCGAACCCGATCGTCGAATTTCTGCGTGCAGGTTTCGCGGAGCGCAAGGCGGTGGTCAGCGTTGACATGGAGGGGTCAACGGTGTTGTTTGACTTTGTGCGAATGTTGCAGATTGATTTGGGGGCTGGGAGTCAGAGATCTATCGCTTGGATTGACGTGAACGGCAGGCCCTTCTTCCCTAAGGCGTTTGTCAGCGAGGATTTGGTGGACCGGTCGGATAGTCCGAAGGTCCAAATCAACCAACGGGTCAATGTATCGGGTCGGGTTTTGGGCAAGAGGCGGACGGTGGAAGAGCCGGCGGATGAGGACGAAGTGACATCGTCGATAAGGCCGCTGAGTTCGGAGTGGCCGAATGTGAGGTTGCTGGGAGGAGTTGAGAGGGTTTACACAGTGTGCAGCAATTTGTTTCTCGCGGGGATGAGGAAGGTCGATCTCTCGGCTGCTGTGACGGCAGTTCATCAGCGCGTTCGTGACGGACCGTTGGATAAGGCTCGATTGGAGGTGTTTCAGAAGCAGATTGAGATCACCAAGGCGGCTCGGGGCTCGGCCAATGTTGTATACGCCTGGTGCGGCGTCTCGGGCAGCGATGTGAGAGGCATTTTGACACATGGGTTCGGCGCACCCAGCAAGGTTTCTGGGACGCAGTCGCATGGCGTCGGTCTTCACCTTTCGCATTTATCGGCGCCTTTCCTGAGTTGTGCGCAGTCGGAACGGGATGATAATGGCGAAAAATATGCTATTTTGTGCCGGGTTATACTAGGCAGTGTTGAAAAAGTAGAGGCAGGTTCTAAGCAATGCTACTAG

Protein Analysis

339

Amino Acids

36.97

Weight (kDa)

9.31

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WWE_5 PF23467 51 - 130 5.2e-25 WWE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013684)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g33460 FvH4_5g33460
malus_domestica MD08G1211300.v1.1
prunus_persica Prupe.1G543300_v2.0.a1
pyrus_communis pycom08g18210 pycom15g35530
rosa_chinensis RchiOBHm_Chr7g0232191
rosa_laevigata RLG00000001378
rosa_multiflora Rmu_co8293403.1_g000001 Rmu_sc0029785.1_g000001
rosa_roxburghii Rroxscaffold_3G00228680
rosa_rugosa Rorug07G0270100
rosa_samantha Rh7AG424700 Rh7BG398800 Rh7CG445200 Rh7DG416700
rosa_wichuraiana Rw7G035130 Rw7G035230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 125
Acc16I TGCGCA 1 cut(s) 915
Acc36I ACCTGC 2 cut(s) 233, 986
AccB1I GGYRCC 1 cut(s) 895
AccI GTMKAC 1 cut(s) 771
AccII CGCG 3 cut(s) 251, 626, 678
AclWI GGATC 2 cut(s) 64, 77
AcoI YGGCCR 2 cut(s) 560, 759
AcsI RAATTY 1 cut(s) 230
AcyI GRCGYC 4 cut(s) 122, 786, 867, 896
AdeI CACNNNGTG 1 cut(s) 606
AfiI CCNNNNNNNGG 3 cut(s) 43, 174, 175
AgeI ACCGGT 1 cut(s) 423
AgsI TTSAA 2 cut(s) 154, 983
AjiI CACGTC 1 cut(s) 370
AjnI CCWGG 2 cut(s) 200, 776
AluBI AGCT 1 cut(s) 99
AluI AGCT 1 cut(s) 99
Alw21I GWGCWC 1 cut(s) 101
Alw26I GTCTC 1 cut(s) 793
AlwI GGATC 2 cut(s) 64, 77
Ama87I CYCGRG 2 cut(s) 750, 790
AoxI GGCC 4 cut(s) 380, 542, 560, 759
ApeKI GCWGC 3 cut(s) 609, 653, 795
ApoI RAATTY 1 cut(s) 230
AsiGI ACCGGT 1 cut(s) 423
AspLEI GCGC 6 cut(s) 164, 258, 678, 833, 898, 916
AspS9I GGNCC 4 cut(s) 381, 421, 442, 689
AsuC2I CCSGG 2 cut(s) 170, 962
AsuHPI GGTGA 3 cut(s) 40, 730, 869
AvaI CYCGRG 2 cut(s) 750, 790
AvaII GGWCC 3 cut(s) 421, 442, 689
AxyI CCTNAGG 1 cut(s) 393
BamHI GGATCC 1 cut(s) 69
BanI GGYRCC 1 cut(s) 895
BanII GRGCYC 2 cut(s) 101, 758
BbsI GAAGAC 2 cut(s) 78, 866
Bbv12I GWGCWC 1 cut(s) 101
BbvCI CCTCAGC 1 cut(s) 91
BbvI GCAGC 3 cut(s) 621, 640, 807
BceAI ACGGC 3 cut(s) 64, 391, 678
BcgI CGANNNNNNTGC 2 cut(s) 929, 963
BciT130I CCWGG 2 cut(s) 202, 778
BcnI CCSGG 2 cut(s) 170, 962
BcoDI GTCTC 1 cut(s) 793
BfaI CTAG 2 cut(s) 971, 1015
BfoI RGCGCY 2 cut(s) 165, 899
BfuAI ACCTGC 2 cut(s) 233, 986
BglI GCCNNNNNGGC 1 cut(s) 170
BglII AGATCT 1 cut(s) 350
BisI GCNGC 8 cut(s) 15, 165, 545, 610, 654, 747, 784, 796
BlsI GCNGC 8 cut(s) 16, 166, 546, 611, 655, 748, 785, 797
Bme1390I CCNGG 4 cut(s) 170, 202, 778, 962
Bme18I GGWCC 3 cut(s) 421, 442, 689
BmeT110I CYCGRG 2 cut(s) 750, 790
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 4 cut(s) 381, 421, 442, 689
BmiI GGNNCC 3 cut(s) 60, 71, 897
BmrFI CCNGG 4 cut(s) 170, 202, 778, 962
BmsI GCATC 1 cut(s) 123
BpiI GAAGAC 2 cut(s) 78, 866
Bpu10I CCTNAGC 1 cut(s) 91
BpuMI CCSGG 2 cut(s) 170, 962
BsaBI GATNNNNATC 1 cut(s) 734
BsaHI GRCGYC 4 cut(s) 122, 786, 867, 896
BsaJI CCNNGG 3 cut(s) 168, 201, 741
BsaWI WCCGGW 1 cut(s) 423
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 174, 175
Bse118I RCCGGY 2 cut(s) 423, 508
Bse21I CCTNAGG 1 cut(s) 393
Bse3DI GCAATG 1 cut(s) 1013
Bse8I GATNNNNATC 1 cut(s) 734
BseBI CCWGG 2 cut(s) 202, 778
BseDI CCNNGG 3 cut(s) 168, 201, 741
BseGI GGATG 4 cut(s) 138, 520, 636, 934
BseJI GATNNNNATC 1 cut(s) 734
BseLI CCNNNNNNNGG 3 cut(s) 43, 174, 175
BseMI GCAATG 1 cut(s) 1013
BseMII CTCAG 3 cut(s) 105, 539, 896
BseRI GAGGAG 1 cut(s) 597
BseXI GCAGC 3 cut(s) 621, 640, 807
BseYI CCCAGC 3 cut(s) 338, 577, 836
BsgI GTGCAG 2 cut(s) 261, 628
Bsh1236I CGCG 3 cut(s) 251, 626, 678
Bsh1285I CGRYCG 2 cut(s) 225, 428
BshFI GGCC 4 cut(s) 382, 544, 562, 761
BshNI GGYRCC 1 cut(s) 895
BshTI ACCGGT 1 cut(s) 423
BsiEI CGRYCG 2 cut(s) 225, 428
BsiHKAI GWGCWC 1 cut(s) 101
BsiHKCI CYCGRG 2 cut(s) 750, 790
BsiSI CCGG 4 cut(s) 170, 424, 509, 961
BslFI GGGAC 2 cut(s) 218, 865
BslI CCNNNNNNNGG 3 cut(s) 43, 174, 175
BsmAI GTCTC 1 cut(s) 793
BsmBI CGTCTC 1 cut(s) 793
BsmFI GGGAC 2 cut(s) 218, 865
BsnI GGCC 4 cut(s) 382, 544, 562, 761
BsoBI CYCGRG 2 cut(s) 750, 790
Bsp1286I GDGCHC 2 cut(s) 101, 758
Bsp143I GATC 5 cut(s) 69, 222, 350, 643, 735
BspANI GGCC 4 cut(s) 382, 544, 562, 761
BspCNI CTCAG 3 cut(s) 104, 540, 897
BspFNI CGCG 3 cut(s) 251, 626, 678
BspLI GGNNCC 3 cut(s) 60, 71, 897
BspMI ACCTGC 2 cut(s) 233, 986
BspPI GGATC 2 cut(s) 64, 77
BspQI GCTCTTC 1 cut(s) 498
BspT107I GGYRCC 1 cut(s) 895
BsrDI GCAATG 1 cut(s) 1013
BsrFI RCCGGY 2 cut(s) 423, 508
BssAI RCCGGY 2 cut(s) 423, 508
BssECI CCNNGG 3 cut(s) 168, 201, 741
BssMI GATC 5 cut(s) 69, 222, 350, 643, 735
BssNAI GTATAC 1 cut(s) 772
BssNI GRCGYC 4 cut(s) 122, 786, 867, 896
BssT1I CCWWGG 1 cut(s) 741
Bst1107I GTATAC 1 cut(s) 772
Bst2UI CCWGG 2 cut(s) 202, 778
Bst4CI ACNGT 5 cut(s) 52, 295, 499, 604, 693
Bst6I CTCTTC 1 cut(s) 498
BstACI GRCGYC 4 cut(s) 122, 786, 867, 896
BstC8I GCNNGC 4 cut(s) 97, 240, 380, 510
BstDEI CTNAG 5 cut(s) 91, 393, 548, 905, 1002
BstEII GGTNACC 1 cut(s) 46
BstF5I GGATG 4 cut(s) 138, 520, 636, 934
BstFNI CGCG 3 cut(s) 251, 626, 678
BstH2I RGCGCY 2 cut(s) 165, 899
BstHHI GCGC 6 cut(s) 164, 258, 678, 833, 898, 916
BstKTI GATC 5 cut(s) 72, 225, 353, 646, 738
BstMAI GTCTC 1 cut(s) 793
BstMBI GATC 5 cut(s) 69, 222, 350, 643, 735
BstMCI CGRYCG 2 cut(s) 225, 428
BstMWI GCNNNNNNNGC 7 cut(s) 11, 170, 248, 319, 662, 792, 837
BstNI CCWGG 2 cut(s) 202, 778
BstPI GGTNACC 1 cut(s) 46
BstSCI CCNGG 4 cut(s) 168, 200, 776, 960
BstUI CGCG 3 cut(s) 251, 626, 678
BstV1I GCAGC 3 cut(s) 621, 640, 807
BstV2I GAAGAC 2 cut(s) 78, 866
BstX2I RGATCY 2 cut(s) 69, 350
BstYI RGATCY 2 cut(s) 69, 350
BstZ17I GTATAC 1 cut(s) 772
Bsu36I CCTNAGG 1 cut(s) 393
BsuRI GGCC 4 cut(s) 382, 544, 562, 761
BtgZI GCGATG 1 cut(s) 813
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 4 cut(s) 138, 520, 636, 934
BtsI GCAGTG 1 cut(s) 982
BtsIMutI CAGTG 2 cut(s) 609, 982
BveI ACCTGC 2 cut(s) 233, 986
Cac8I GCNNGC 4 cut(s) 97, 240, 380, 510
CfoI GCGC 6 cut(s) 164, 258, 678, 833, 898, 916
Cfr10I RCCGGY 2 cut(s) 423, 508
Cfr13I GGNCC 4 cut(s) 381, 421, 442, 689
CpoI CGGWCCG 1 cut(s) 689
CseI GACGC 3 cut(s) 775, 856, 862
CspAI ACCGGT 1 cut(s) 423
CspI CGGWCCG 1 cut(s) 689
CviAII CATG 3 cut(s) 280, 821, 863
DdeI CTNAG 5 cut(s) 91, 393, 548, 905, 1002
DinI GGCGCC 1 cut(s) 897
DpnI GATC 5 cut(s) 71, 224, 352, 645, 737
DpnII GATC 5 cut(s) 69, 222, 350, 643, 735
DraIII CACNNNGTG 1 cut(s) 606
EaeI YGGCCR 2 cut(s) 560, 759
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
EciI GGCGGA 2 cut(s) 508, 527
Ecl136II GAGCTC 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 741
Eco24I GRGCYC 2 cut(s) 101, 758
Eco47I GGWCC 3 cut(s) 421, 442, 689
Eco53kI GAGCTC 1 cut(s) 99
Eco81I CCTNAGG 1 cut(s) 393
Eco88I CYCGRG 2 cut(s) 750, 790
Eco91I GGTNACC 1 cut(s) 46
EcoICRI GAGCTC 1 cut(s) 99
EcoO109I RGGNCCY 1 cut(s) 381
EcoO65I GGTNACC 1 cut(s) 46
EcoRII CCWGG 2 cut(s) 200, 776
EcoT14I CCWWGG 1 cut(s) 741
EcoT38I GRGCYC 2 cut(s) 101, 758
EgeI GGCGCC 1 cut(s) 897
EheI GGCGCC 1 cut(s) 897
ErhI CCWWGG 1 cut(s) 741
Esp3I CGTCTC 1 cut(s) 793
FaeI CATG 3 cut(s) 283, 824, 866
FaiI YATR 7 cut(s) 182, 281, 772, 822, 864, 948, 968
FaqI GGGAC 2 cut(s) 218, 865
FatI CATG 3 cut(s) 279, 820, 862
FauI CCCGC 1 cut(s) 619
FblI GTMKAC 1 cut(s) 771
Fnu4HI GCNGC 8 cut(s) 15, 165, 545, 610, 654, 747, 784, 796
FokI GGATG 4 cut(s) 145, 527, 643, 941
FriOI GRGCYC 2 cut(s) 101, 758
Fsp4HI GCNGC 8 cut(s) 15, 165, 545, 610, 654, 747, 784, 796
FspBI CTAG 2 cut(s) 971, 1015
FspI TGCGCA 1 cut(s) 915
GlaI GCGC 6 cut(s) 163, 257, 677, 832, 897, 915
GluI GCNGC 8 cut(s) 15, 165, 545, 610, 654, 747, 784, 796
GsaI CCCAGC 3 cut(s) 342, 581, 840
HaeII RGCGCY 2 cut(s) 165, 899
HaeIII GGCC 4 cut(s) 382, 544, 562, 761
HapII CCGG 4 cut(s) 170, 424, 509, 961
HgaI GACGC 3 cut(s) 775, 856, 862
HhaI GCGC 6 cut(s) 164, 258, 678, 833, 898, 916
Hin1I GRCGYC 4 cut(s) 122, 786, 867, 896
Hin1II CATG 3 cut(s) 283, 824, 866
Hin6I GCGC 6 cut(s) 162, 256, 676, 831, 896, 914
HinP1I GCGC 6 cut(s) 162, 256, 676, 831, 896, 914
HincII GTYRAC 2 cut(s) 277, 291
HindII GTYRAC 2 cut(s) 277, 291
HinfI GANTC 2 cut(s) 197, 343
HpaII CCGG 4 cut(s) 170, 424, 509, 961
HphI GGTGA 3 cut(s) 40, 730, 869
Hpy166II GTNNAC 6 cut(s) 277, 291, 373, 421, 598, 772
Hpy188I TCNGA 9 cut(s) 63, 120, 132, 348, 430, 438, 556, 723, 922
Hpy188III TCNNGA 3 cut(s) 190, 683, 904
Hpy8I GTNNAC 6 cut(s) 277, 291, 373, 421, 598, 772
Hpy99I CGWCG 4 cut(s) 85, 230, 538, 872
HpyAV CCTTC 4 cut(s) 58, 394, 433, 631
HpyCH4III ACNGT 5 cut(s) 52, 295, 499, 604, 693
HpyCH4IV ACGT 2 cut(s) 122, 369
HpyCH4V TGCA 5 cut(s) 114, 136, 242, 322, 609
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 170, 248, 319, 662, 792, 837
HpyF3I CTNAG 5 cut(s) 91, 393, 548, 905, 1002
HpySE526I ACGT 2 cut(s) 122, 369
Hsp92I GRCGYC 4 cut(s) 122, 786, 867, 896
Hsp92II CATG 3 cut(s) 283, 824, 866
HspAI GCGC 6 cut(s) 162, 256, 676, 831, 896, 914
KasI GGCGCC 1 cut(s) 895
KroI GCCGGC 1 cut(s) 508
KroNI GCCGGC 1 cut(s) 510
Kzo9I GATC 5 cut(s) 69, 222, 350, 643, 735
LguI GCTCTTC 1 cut(s) 498
LmnI GCTCC 2 cut(s) 159, 253
Lsp1109I GCAGC 3 cut(s) 621, 640, 807
LweI GCATC 1 cut(s) 123
MaeI CTAG 2 cut(s) 971, 1015
MaeII ACGT 2 cut(s) 122, 369
MaeIII GTNAC 4 cut(s) 46, 526, 658, 683
MalI GATC 5 cut(s) 71, 224, 352, 645, 737
MboI GATC 5 cut(s) 69, 222, 350, 643, 735
MboII GAAGA 5 cut(s) 78, 178, 379, 515, 866
MflI RGATCY 2 cut(s) 69, 350
MhlI GDGCHC 2 cut(s) 101, 758
MluCI AATT 3 cut(s) 148, 230, 613
Mly113I GGCGCC 1 cut(s) 896
MlyI GAGTC 2 cut(s) 206, 352
MmeI TCCRAC 3 cut(s) 408, 675, 900
MroNI GCCGGC 1 cut(s) 508
MspA1I CMGCKG 1 cut(s) 547
MspI CCGG 4 cut(s) 170, 424, 509, 961
MspR9I CCNGG 4 cut(s) 170, 202, 778, 962
MvaI CCWGG 2 cut(s) 202, 778
MvnI CGCG 3 cut(s) 251, 626, 678
MwoI GCNNNNNNNGC 7 cut(s) 11, 170, 248, 319, 662, 792, 837
NaeI GCCGGC 1 cut(s) 510
NarI GGCGCC 1 cut(s) 896
NciI CCSGG 2 cut(s) 170, 962
NdeII GATC 5 cut(s) 69, 222, 350, 643, 735
NgoMIV GCCGGC 1 cut(s) 508
NlaIII CATG 3 cut(s) 283, 824, 866
NlaIV GGNNCC 3 cut(s) 60, 71, 897
NmeAIII GCCGAG 2 cut(s) 629, 737
NmuCI GTSAC 4 cut(s) 46, 526, 658, 683
NsbI TGCGCA 1 cut(s) 915
PciSI GCTCTTC 1 cut(s) 498
PcsI WCGNNNNNNNCGW 2 cut(s) 434, 560
PdiI GCCGGC 1 cut(s) 510
PflFI GACNNNGTC 2 cut(s) 532, 856
PinAI ACCGGT 1 cut(s) 423
PkrI GCNGC 8 cut(s) 16, 166, 546, 611, 655, 748, 785, 797
Ple19I CGATCG 1 cut(s) 225
PleI GAGTC 2 cut(s) 205, 351
PluTI GGCGCC 1 cut(s) 899
PpsI GAGTC 2 cut(s) 205, 351
Psp124BI GAGCTC 1 cut(s) 101
Psp6I CCWGG 2 cut(s) 200, 776
PspEI GGTNACC 1 cut(s) 46
PspFI CCCAGC 3 cut(s) 338, 577, 836
PspGI CCWGG 2 cut(s) 200, 776
PspN4I GGNNCC 3 cut(s) 60, 71, 897
PspPI GGNCC 4 cut(s) 381, 421, 442, 689
PsuI RGATCY 2 cut(s) 69, 350
PsyI GACNNNGTC 2 cut(s) 532, 856
PvuI CGATCG 1 cut(s) 225
Rsr2I CGGWCCG 1 cut(s) 689
RsrII CGGWCCG 1 cut(s) 689
SacI GAGCTC 1 cut(s) 101
SapI GCTCTTC 1 cut(s) 498
SatI GCNGC 8 cut(s) 15, 165, 545, 610, 654, 747, 784, 796
Sau3AI GATC 5 cut(s) 69, 222, 350, 643, 735
Sau96I GGNCC 4 cut(s) 381, 421, 442, 689
SchI GAGTC 2 cut(s) 206, 352
ScrFI CCNGG 4 cut(s) 170, 202, 778, 962
SduI GDGCHC 2 cut(s) 101, 758
SfaNI GCATC 1 cut(s) 123
SfoI GGCGCC 1 cut(s) 897
SinI GGWCC 3 cut(s) 421, 442, 689
Sse9I AATT 3 cut(s) 148, 230, 613
SspDI GGCGCC 1 cut(s) 895
SspMI CTAG 2 cut(s) 971, 1015
SstI GAGCTC 1 cut(s) 101
StyD4I CCNGG 4 cut(s) 168, 200, 776, 960
StyI CCWWGG 1 cut(s) 741
TaaI ACNGT 5 cut(s) 52, 295, 499, 604, 693
TaiI ACGT 2 cut(s) 125, 372
TaqI TCGA 4 cut(s) 228, 536, 642, 706
TaqII GACCGA 2 cut(s) 34, 860
TasI AATT 3 cut(s) 148, 230, 613
TauI GCSGC 5 cut(s) 17, 167, 547, 749, 786
TscAI CASTG 2 cut(s) 609, 982
TseFI GTSAC 4 cut(s) 46, 526, 658, 683
TseI GCWGC 3 cut(s) 609, 653, 795
Tsp45I GTSAC 4 cut(s) 46, 526, 658, 683
TspDTI ATGAA 1 cut(s) 659
TspGWI ACGGA 1 cut(s) 702
TspRI CASTG 2 cut(s) 609, 982
Tth111I GACNNNGTC 2 cut(s) 532, 856
VpaK11BI GGWCC 3 cut(s) 421, 442, 689
XapI RAATTY 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 771
XspI CTAG 2 cut(s) 971, 1015
ZraI GACGTC 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.