Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0006439

Physical Location & Seq

Genomic Coordinates Forward (+)

27770278 .. 27775034

Gene Structure 4757 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1214900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGGGCTGTCTTTGCTCGAAAGCAACATCGGAAGAGGGAAAGGTGGAGCAATCCGAGATAGCAGAACGCCCGTTGAAGGATTATTCGGTTCAGTTGATTGCTCCTACTCCCTCATTGAGAGATGACTTTGTCACAGAAGTAGGCGGTGGTGGTGATGGGGCTGAGGTATCAGTGCGCAGGTCATCAAGAACAGCCTCAAAAGCAAACGTGGGGAACAATGGTAGTAAGAATGAAGACCATCAAAGAGGGGCAACACTGGGTTTAGGAGCAAGTGGAAGGCAAACGGTGATGTCTAGGATAGTGAGCATGCCGCATGGGGCACAAGGAGAACAAACTGCTGCTGGATGGCCGTCATGGCTAACTTCGGTTGCTGGAGATGCCATCAAAGGGTGGGTGCCTCGACGGGCAGACTCCTTTGAAAAGTTAGACAAAATTGGACAAGGAACTTATAGCAGTGTATACAAGGCTCGCGACCTGGAAACTGGTAAAATCGTTGCATTGAAGAAAGTACGATTCGTAAATATGGACCCAGAAAGTGTTCGTTTTATGGCAAGAGAAATCTATATATTACGTAGACTTGATCACCCAAATGTTATGAAGCTCGAGGGTCTAGTCACTTCAAGAATGTCATGCAGCTTATACCTTGTCTTCGAGTATATGGAGCATGACCTTGCTGGTCTAGCAGCAACACGTGGCATCAAGTTTACTGAACCGCAGATAAAATGTTATATGCAACAACTGCTTCGCGGACTTGAATACTGTCATAGTCAAGGTGTCCTGCACCGAGATATCAAGGGTTCAAATCTTTTACTTGACAATAATGGAGCTCTCAAGATTGGCGACTTTGGTCTGGCGACCTTTTATGAGCCTGACCAAAACCAGGCATTAACCAGTCGTGTTGTGACTCTGTGGTATAGGGCACCTGAACTTTTACTTGGTTCTACTGAGTATGGAGTTTCTATAGATCTATGGAGCACCGGCTGTATCCTTGCGGAAATGTATGCTGGAAAGCCTATCATGCCCGGCAGAACAGAGGTGGAACAAATGCATAAAATTTTTAAGCTTTGTGGTTCGCCATCTGAGGAATTCTGGAAGAAAACAAAGTTACCCCTTGCAACTAGTTTCAAACCTCAACAACCTTACAAGCGTCGTATTGCTGATACTTTCAAAGACGTCCCTCCTTCAGCTTTGGAGCTTATTGAAAAACTCCTTGCAATAGAACCAGAGACTCGGGGATCGGCTGCTTCAGCGCTTAGTAGTGAGTTCTTCAGTACACATCCTCTACCTTGTGATCCCTCAGATCTACCAAAATATCGTCCGAGCAAAGAGTTTGATGCAAAGCTTCGGGATGAGGAAAAACGGCGAAAAGCCGATGCATTTAAAGGGCGTGGTCCTGAATCTGTTAGAAGGGGTTCAAGAGATACCAAGGCACCATCGCCAGAATTTAATACTCCGGGAGAGGGACAGAGAAACCGGAAAACCACAGGTCACAAGTACATGTCACAGCGAGAAACTGTCTCCGGCTTTGCAATGGAACCACATGGAGGAGCTAGGTCAAATGGGTACTCTCATTGTAGTACAATGGTACACCCTAGTGCAGTAGGATCGTCATTGAATAAGACAGCAGGTTCTACAAGTAGGCCAGAGCTAAGGTCGCAGAGATCTCATATGCCTCAAGCTGCAGCAGACTTATTAAGTTCTTCCAGTAAGAAAAAAGAAACTGTGGCTGGCAAAGAATCCAGAACGGGCTATGTACCTAAGAACAGAATCCATTGCTCTGGACCATTGGTGCCTCCGGGGGGAAATCTTGAGGACATGCTCAAAGAGCACGAAAGACAAATCCAGCAGGCTGTACGTAAAGCACGAGCGCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0071944 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

625

Amino Acids

68.83

Weight (kDa)

9.34

Isoelectric Point (pI)

51.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	139 - 423	3.1e-70	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	142 - 352	6.6e-32	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006439)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33770 AT1G71530 AT1G71530 AT1G71530 AT1G71530 AT4G10010 AT4G10010
fragaria_vesca	FvH4_5g33160 FvH4_5g33160
malus_domestica	MD08G1214900.v1.1 MD15G1397900.v1.1
prunus_persica	Prupe.1G546300_v2.0.a1 Prupe.1G546300_v2.0.a1
pyrus_communis	pycom15g35760
rosa_chinensis	RchiOBHm_Chr7g0231421
rosa_laevigata	RLG00000001418
rosa_multiflora	Rmu_sc0003396.1_g000031 Rmu_sc0010066.1_g000001
rosa_roxburghii	Rroxscaffold_3G00229330
rosa_rugosa	Rorug07G0266500 Rorug07G0266500
rosa_samantha	Rh7AG420800 Rh7BG395200 Rh7CG440900 Rh7DG413400
rosa_wichuraiana	Rw7G034720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1176
Acc16I	TGCGCA	1 cut(s)	176
Acc36I	ACCTGC	2 cut(s)	168, 1616
AccB1I	GGYRCC	4 cut(s)	394, 919, 1429, 1789
AccI	GTMKAC	2 cut(s)	459, 574
AccII	CGCG	3 cut(s)	471, 747, 1870
AciI	CCGC	5 cut(s)	144, 311, 713, 747, 992
AclWI	GGATC	3 cut(s)	1243, 1286, 1612
AcoI	YGGCCR	1 cut(s)	347
AcsI	RAATTY	3 cut(s)	1053, 1085, 1442
AcuI	CTGAAG	3 cut(s)	1167, 1230, 1252
AcvI	CACGTG	1 cut(s)	692
AcyI	GRCGYC	1 cut(s)	1173
AfaI	GTAC	8 cut(s)	510, 1273, 1496, 1565, 1579, 1587, 1755, 1854
AfeI	AGCGCT	1 cut(s)	1251
AfiI	CCNNNNNNNGG	6 cut(s)	76, 387, 880, 1460, 1544, 1799
AflIII	ACRYGT	2 cut(s)	689, 1497
AhlI	ACTAGT	1 cut(s)	1118
AjnI	CCWGG	2 cut(s)	474, 879
AjuI	GAANNNNNNNTTGG	2 cut(s)	918, 950
AleI	CACNNNNGTG	1 cut(s)	1593
Alw21I	GWGCWC	3 cut(s)	829, 977, 1830
Alw26I	GTCTC	2 cut(s)	1220, 1522
AlwI	GGATC	3 cut(s)	1243, 1286, 1612
Ama87I	CYCGRG	2 cut(s)	602, 1230
Aor51HI	AGCGCT	1 cut(s)	1251
AoxI	GGCC	2 cut(s)	347, 1640
ApeKI	GCWGC	6 cut(s)	338, 633, 683, 1241, 1679, 1682
ApoI	RAATTY	3 cut(s)	1053, 1085, 1442
Asp700I	GAANNNNTTC	2 cut(s)	537, 1411
AspLEI	GCGC	3 cut(s)	177, 1252, 1870
AspS9I	GGNCC	3 cut(s)	526, 1391, 1781
AsuC2I	CCSGG	3 cut(s)	1023, 1455, 1797
AsuHPI	GGTGA	3 cut(s)	164, 298, 575
AvaI	CYCGRG	2 cut(s)	602, 1230
AvaII	GGWCC	3 cut(s)	526, 1391, 1781
BaeGI	GKGCMC	2 cut(s)	322, 922
BanI	GGYRCC	4 cut(s)	394, 919, 1429, 1789
BanII	GRGCYC	1 cut(s)	829
BauI	CACGAG	1 cut(s)	1863
BbrPI	CACGTG	1 cut(s)	692
BbsI	GAAGAC	2 cut(s)	240, 640
Bbv12I	GWGCWC	3 cut(s)	829, 977, 1830
BbvCI	CCTCAGC	1 cut(s)	162
BbvI	GCAGC	6 cut(s)	325, 645, 695, 1228, 1666, 1694
BccI	CCATC	6 cut(s)	149, 246, 339, 389, 1084, 1441
BceAI	ACGGC	2 cut(s)	334, 1376
BcgI	CGANNNNNNTGC	2 cut(s)	1587, 1621
BciT130I	CCWGG	2 cut(s)	476, 881
BciVI	GTATCC	1 cut(s)	995
BclI	TGATCA	1 cut(s)	580
BcnI	CCSGG	3 cut(s)	1023, 1455, 1797
BcoDI	GTCTC	2 cut(s)	1220, 1522
BcuI	ACTAGT	1 cut(s)	1118
BfaI	CTAG	6 cut(s)	294, 611, 680, 1119, 1551, 1593
BfmI	CTRYAG	2 cut(s)	960, 1680
BfoI	RGCGCY	1 cut(s)	1253
BfuAI	ACCTGC	2 cut(s)	168, 1616
BfuI	GTATCC	1 cut(s)	995
BglI	GCCNNNNNGGC	1 cut(s)	355
BglII	AGATCT	3 cut(s)	964, 1300, 1661
BisI	GCNGC	7 cut(s)	311, 339, 634, 684, 1242, 1680, 1683
BlsI	GCNGC	7 cut(s)	312, 340, 635, 685, 1243, 1681, 1684
Bme1390I	CCNGG	5 cut(s)	476, 881, 1023, 1455, 1797
Bme18I	GGWCC	3 cut(s)	526, 1391, 1781
BmeT110I	CYCGRG	2 cut(s)	602, 1230
BmgT120I	GGNCC	3 cut(s)	526, 1391, 1781
BmiI	GGNNCC	6 cut(s)	396, 528, 921, 1431, 1536, 1791
BmrFI	CCNGG	5 cut(s)	476, 881, 1023, 1455, 1797
BmrI	ACTGGG	1 cut(s)	266
BmsI	GCATC	4 cut(s)	367, 705, 1324, 1363
BmuI	ACTGGG	1 cut(s)	266
BoxI	GACNNNNGTC	1 cut(s)	846
BpiI	GAAGAC	2 cut(s)	240, 640
BpmI	CTGGAG	1 cut(s)	393
Bpu10I	CCTNAGC	2 cut(s)	162, 1649
BpuEI	CTTGAG	3 cut(s)	815, 1659, 1829
BpuMI	CCSGG	3 cut(s)	1023, 1455, 1797
BsaAI	YACGTR	3 cut(s)	572, 692, 1856
BsaHI	GRCGYC	1 cut(s)	1173
BsaJI	CCNNGG	2 cut(s)	1425, 1796
BsaWI	WCCGGW	1 cut(s)	1473
Bsc4I	CCNNNNNNNGG	6 cut(s)	76, 387, 880, 1460, 1544, 1799
Bse118I	RCCGGY	1 cut(s)	977
Bse1I	ACTGG	4 cut(s)	261, 487, 891, 1704
Bse3DI	GCAATG	2 cut(s)	1536, 1771
BseBI	CCWGG	2 cut(s)	476, 881
BseDI	CCNNGG	2 cut(s)	1425, 1796
BseGI	GGATG	3 cut(s)	350, 1276, 1354
BseLI	CCNNNNNNNGG	6 cut(s)	76, 387, 880, 1460, 1544, 1799
BseMI	GCAATG	2 cut(s)	1536, 1771
BseMII	CTCAG	4 cut(s)	153, 936, 1071, 1311
BseNI	ACTGG	4 cut(s)	261, 487, 891, 1704
BseRI	GAGGAG	1 cut(s)	1560
BseSI	GKGCMC	2 cut(s)	322, 922
BseXI	GCAGC	6 cut(s)	325, 645, 695, 1228, 1666, 1694
BsgI	GTGCAG	2 cut(s)	764, 1617
Bsh1236I	CGCG	3 cut(s)	471, 747, 1870
BshFI	GGCC	2 cut(s)	349, 1642
BshNI	GGYRCC	4 cut(s)	394, 919, 1429, 1789
BsiHKAI	GWGCWC	3 cut(s)	829, 977, 1830
BsiHKCI	CYCGRG	2 cut(s)	602, 1230
BsiSI	CCGG	6 cut(s)	978, 1023, 1454, 1474, 1521, 1796
BslFI	GGGAC	2 cut(s)	1160, 1476
BslI	CCNNNNNNNGG	6 cut(s)	76, 387, 880, 1460, 1544, 1799
BsmAI	GTCTC	2 cut(s)	1220, 1522
BsmFI	GGGAC	2 cut(s)	1160, 1476
BsnI	GGCC	2 cut(s)	349, 1642
BsoBI	CYCGRG	2 cut(s)	602, 1230
Bsp1286I	GDGCHC	5 cut(s)	322, 829, 922, 977, 1830
Bsp143I	GATC	7 cut(s)	580, 964, 1235, 1291, 1300, 1604, 1661
Bsp68I	TCGCGA	1 cut(s)	471
BspACI	CCGC	5 cut(s)	144, 311, 713, 747, 992
BspANI	GGCC	2 cut(s)	349, 1642
BspCNI	CTCAG	4 cut(s)	154, 937, 1072, 1310
BspFNI	CGCG	3 cut(s)	471, 747, 1870
BspLI	GGNNCC	6 cut(s)	396, 528, 921, 1431, 1536, 1791
BspMAI	CTGCAG	1 cut(s)	1684
BspMI	ACCTGC	2 cut(s)	168, 1616
BspPI	GGATC	3 cut(s)	1243, 1286, 1612
BspT107I	GGYRCC	4 cut(s)	394, 919, 1429, 1789
BsrDI	GCAATG	2 cut(s)	1536, 1771
BsrFI	RCCGGY	1 cut(s)	977
BsrI	ACTGG	4 cut(s)	261, 487, 891, 1704
BssAI	RCCGGY	1 cut(s)	977
BssECI	CCNNGG	2 cut(s)	1425, 1796
BssMI	GATC	7 cut(s)	580, 964, 1235, 1291, 1300, 1604, 1661
BssNAI	GTATAC	1 cut(s)	460
BssNI	GRCGYC	1 cut(s)	1173
BssSI	CACGAG	1 cut(s)	1863
BssT1I	CCWWGG	1 cut(s)	1425
Bst1107I	GTATAC	1 cut(s)	460
Bst2BI	CACGAG	1 cut(s)	1863
Bst2UI	CCWGG	2 cut(s)	476, 881
Bst4CI	ACNGT	4 cut(s)	286, 761, 1516, 1723
Bst6I	CTCTTC	1 cut(s)	27
BstACI	GRCGYC	1 cut(s)	1173
BstAPI	GCANNNNNTGC	1 cut(s)	739
BstBAI	YACGTR	3 cut(s)	572, 692, 1856
BstC8I	GCNNGC	4 cut(s)	308, 469, 1729, 1848
BstDEI	CTNAG	7 cut(s)	162, 945, 1080, 1253, 1297, 1649, 1758
BstF5I	GGATG	3 cut(s)	350, 1276, 1354
BstFNI	CGCG	3 cut(s)	471, 747, 1870
BstH2I	RGCGCY	1 cut(s)	1253
BstHHI	GCGC	3 cut(s)	177, 1252, 1870
BstKTI	GATC	7 cut(s)	583, 967, 1238, 1294, 1303, 1607, 1664
BstMAI	GTCTC	2 cut(s)	1220, 1522
BstMBI	GATC	7 cut(s)	580, 964, 1235, 1291, 1300, 1604, 1661
BstNI	CCWGG	2 cut(s)	476, 881
BstNSI	RCATGY	3 cut(s)	310, 1501, 1819
BstPAI	GACNNNNGTC	1 cut(s)	846
BstSCI	CCNGG	5 cut(s)	474, 879, 1021, 1453, 1795
BstSFI	CTRYAG	2 cut(s)	960, 1680
BstSLI	GKGCMC	2 cut(s)	322, 922
BstSNI	TACGTA	2 cut(s)	572, 1856
BstUI	CGCG	3 cut(s)	471, 747, 1870
BstV1I	GCAGC	6 cut(s)	325, 645, 695, 1228, 1666, 1694
BstV2I	GAAGAC	2 cut(s)	240, 640
BstX2I	RGATCY	3 cut(s)	964, 1300, 1661
BstXI	CCANNNNNNTGG	1 cut(s)	1778
BstYI	RGATCY	3 cut(s)	964, 1300, 1661
BstZ17I	GTATAC	1 cut(s)	460
BsuI	GTATCC	1 cut(s)	995
BsuRI	GGCC	2 cut(s)	349, 1642
BtgZI	GCGATG	1 cut(s)	1419
BtsCI	GGATG	3 cut(s)	350, 1276, 1354
BtsI	GCAGTG	1 cut(s)	460
BtsIMutI	CAGTG	3 cut(s)	177, 254, 460
BtuMI	TCGCGA	1 cut(s)	471
BveI	ACCTGC	2 cut(s)	168, 1616
Cac8I	GCNNGC	4 cut(s)	308, 469, 1729, 1848
CfoI	GCGC	3 cut(s)	177, 1252, 1870
Cfr10I	RCCGGY	1 cut(s)	977
Cfr13I	GGNCC	3 cut(s)	526, 1391, 1781
CseI	GACGC	1 cut(s)	1136
Csp6I	GTAC	8 cut(s)	509, 1272, 1495, 1564, 1578, 1586, 1754, 1853
CviAII	CATG	9 cut(s)	307, 314, 354, 630, 665, 1018, 1498, 1541, 1816
CviQI	GTAC	8 cut(s)	509, 1272, 1495, 1564, 1578, 1586, 1754, 1853
DdeI	CTNAG	7 cut(s)	162, 945, 1080, 1253, 1297, 1649, 1758
DpnI	GATC	7 cut(s)	582, 966, 1237, 1293, 1302, 1606, 1663
DpnII	GATC	7 cut(s)	580, 964, 1235, 1291, 1300, 1604, 1661
DraI	TTTAAA	1 cut(s)	1381
EaeI	YGGCCR	1 cut(s)	347
Eam1104I	CTCTTC	1 cut(s)	27
EarI	CTCTTC	1 cut(s)	27
Ecl136II	GAGCTC	1 cut(s)	827
Eco105I	TACGTA	2 cut(s)	572, 1856
Eco130I	CCWWGG	1 cut(s)	1425
Eco24I	GRGCYC	1 cut(s)	829
Eco32I	GATATC	1 cut(s)	790
Eco47I	GGWCC	3 cut(s)	526, 1391, 1781
Eco47III	AGCGCT	1 cut(s)	1251
Eco53kI	GAGCTC	1 cut(s)	827
Eco57I	CTGAAG	3 cut(s)	1167, 1230, 1252
Eco72I	CACGTG	1 cut(s)	692
Eco88I	CYCGRG	2 cut(s)	602, 1230
EcoICRI	GAGCTC	1 cut(s)	827
EcoRI	GAATTC	1 cut(s)	1085
EcoRII	CCWGG	2 cut(s)	474, 879
EcoRV	GATATC	1 cut(s)	790
EcoT14I	CCWWGG	1 cut(s)	1425
EcoT22I	ATGCAT	2 cut(s)	1050, 1378
EcoT38I	GRGCYC	1 cut(s)	829
ErhI	CCWWGG	1 cut(s)	1425
FaeI	CATG	9 cut(s)	310, 317, 357, 633, 668, 1021, 1501, 1544, 1819
FaqI	GGGAC	2 cut(s)	1160, 1476
FatI	CATG	9 cut(s)	306, 313, 353, 629, 664, 1017, 1497, 1540, 1815
FauNDI	CATATG	1 cut(s)	1668
FbaI	TGATCA	1 cut(s)	580
FblI	GTMKAC	2 cut(s)	459, 574
Fnu4HI	GCNGC	7 cut(s)	311, 339, 634, 684, 1242, 1680, 1683
FokI	GGATG	3 cut(s)	357, 1263, 1361
FriOI	GRGCYC	1 cut(s)	829
Fsp4HI	GCNGC	7 cut(s)	311, 339, 634, 684, 1242, 1680, 1683
FspBI	CTAG	6 cut(s)	294, 611, 680, 1119, 1551, 1593
FspI	TGCGCA	1 cut(s)	176
GlaI	GCGC	3 cut(s)	176, 1251, 1869
GluI	GCNGC	7 cut(s)	311, 339, 634, 684, 1242, 1680, 1683
GsuI	CTGGAG	1 cut(s)	393
HaeII	RGCGCY	1 cut(s)	1253
HaeIII	GGCC	2 cut(s)	349, 1642
HapII	CCGG	6 cut(s)	978, 1023, 1454, 1474, 1521, 1796
HgaI	GACGC	1 cut(s)	1136
HhaI	GCGC	3 cut(s)	177, 1252, 1870
Hin1I	GRCGYC	1 cut(s)	1173
Hin1II	CATG	9 cut(s)	310, 317, 357, 633, 668, 1021, 1501, 1544, 1819
Hin6I	GCGC	3 cut(s)	175, 1250, 1868
HinP1I	GCGC	3 cut(s)	175, 1250, 1868
HindIII	AAGCTT	2 cut(s)	1061, 1340
HinfI	GANTC	7 cut(s)	410, 513, 904, 1228, 1397, 1736, 1767
HpaII	CCGG	6 cut(s)	978, 1023, 1454, 1474, 1521, 1796
HphI	GGTGA	3 cut(s)	164, 298, 575
Hpy166II	GTNNAC	5 cut(s)	460, 575, 705, 1274, 1588
Hpy188I	TCNGA	5 cut(s)	31, 55, 1081, 1300, 1320
Hpy8I	GTNNAC	5 cut(s)	460, 575, 705, 1274, 1588
Hpy99I	CGWCG	2 cut(s)	405, 1152
HpyAV	CCTTC	4 cut(s)	70, 270, 1191, 1401
HpyCH4III	ACNGT	4 cut(s)	286, 761, 1516, 1723
HpyCH4IV	ACGT	5 cut(s)	207, 571, 691, 1173, 1855
HpyF3I	CTNAG	7 cut(s)	162, 945, 1080, 1253, 1297, 1649, 1758
HpySE526I	ACGT	5 cut(s)	207, 571, 691, 1173, 1855
Hsp92I	GRCGYC	1 cut(s)	1173
Hsp92II	CATG	9 cut(s)	310, 317, 357, 633, 668, 1021, 1501, 1544, 1819
HspAI	GCGC	3 cut(s)	175, 1250, 1868
Ksp22I	TGATCA	1 cut(s)	580
Kzo9I	GATC	7 cut(s)	580, 964, 1235, 1291, 1300, 1604, 1661
LmnI	GCTCC	8 cut(s)	46, 106, 266, 661, 824, 972, 1192, 1547
Lsp1109I	GCAGC	6 cut(s)	325, 645, 695, 1228, 1666, 1694
LweI	GCATC	4 cut(s)	367, 705, 1324, 1363
MaeI	CTAG	6 cut(s)	294, 611, 680, 1119, 1551, 1593
MaeII	ACGT	5 cut(s)	207, 571, 691, 1173, 1855
MaeIII	GTNAC	6 cut(s)	130, 613, 901, 1104, 1487, 1500
MalI	GATC	7 cut(s)	582, 966, 1237, 1293, 1302, 1606, 1663
MboI	GATC	7 cut(s)	580, 964, 1235, 1291, 1300, 1604, 1661
MboII	GAAGA	7 cut(s)	44, 245, 514, 640, 1105, 1258, 1692
MflI	RGATCY	3 cut(s)	964, 1300, 1661
MhlI	GDGCHC	5 cut(s)	322, 829, 922, 977, 1830
MluCI	AATT	4 cut(s)	432, 1053, 1085, 1442
MlyI	GAGTC	3 cut(s)	404, 898, 1222
Mph1103I	ATGCAT	2 cut(s)	1050, 1378
MroXI	GAANNNNTTC	2 cut(s)	537, 1411
MseI	TTAA	5 cut(s)	887, 1059, 1380, 1446, 1694
MslI	CAYNNNNRTG	2 cut(s)	588, 1593
MspI	CCGG	6 cut(s)	978, 1023, 1454, 1474, 1521, 1796
MspR9I	CCNGG	5 cut(s)	476, 881, 1023, 1455, 1797
MvaI	CCWGG	2 cut(s)	476, 881
MvnI	CGCG	3 cut(s)	471, 747, 1870
NciI	CCSGG	3 cut(s)	1023, 1455, 1797
NdeI	CATATG	1 cut(s)	1668
NdeII	GATC	7 cut(s)	580, 964, 1235, 1291, 1300, 1604, 1661
NlaIII	CATG	9 cut(s)	310, 317, 357, 633, 668, 1021, 1501, 1544, 1819
NlaIV	GGNNCC	6 cut(s)	396, 528, 921, 1431, 1536, 1791
NmuCI	GTSAC	5 cut(s)	130, 613, 901, 1487, 1500
NruI	TCGCGA	1 cut(s)	471
NsbI	TGCGCA	1 cut(s)	176
NsiI	ATGCAT	2 cut(s)	1050, 1378
NspI	RCATGY	3 cut(s)	310, 1501, 1819
OliI	CACNNNNGTG	1 cut(s)	1593
PaeI	GCATGC	1 cut(s)	310
PaeR7I	CTCGAG	1 cut(s)	602
PciI	ACATGT	1 cut(s)	1497
PcsI	WCGNNNNNNNCGW	1 cut(s)	1861
PdmI	GAANNNNTTC	2 cut(s)	537, 1411
PfeI	GAWTC	4 cut(s)	513, 1397, 1736, 1767
PflFI	GACNNNGTC	1 cut(s)	128
PfoI	TCCNGGA	1 cut(s)	1453
PkrI	GCNGC	7 cut(s)	312, 340, 635, 685, 1243, 1681, 1684
PleI	GAGTC	3 cut(s)	404, 898, 1222
PmaCI	CACGTG	1 cut(s)	692
PmlI	CACGTG	1 cut(s)	692
PpsI	GAGTC	3 cut(s)	404, 898, 1222
Ppu21I	YACGTR	3 cut(s)	572, 692, 1856
PscI	ACATGT	1 cut(s)	1497
PshAI	GACNNNNGTC	1 cut(s)	846
Psp124BI	GAGCTC	1 cut(s)	829
Psp6I	CCWGG	2 cut(s)	474, 879
PspCI	CACGTG	1 cut(s)	692
PspGI	CCWGG	2 cut(s)	474, 879
PspN4I	GGNNCC	6 cut(s)	396, 528, 921, 1431, 1536, 1791
PspPI	GGNCC	3 cut(s)	526, 1391, 1781
PspXI	VCTCGAGB	1 cut(s)	602
PstI	CTGCAG	1 cut(s)	1684
PsuI	RGATCY	3 cut(s)	964, 1300, 1661
PsyI	GACNNNGTC	1 cut(s)	128
RruI	TCGCGA	1 cut(s)	471
RsaI	GTAC	8 cut(s)	510, 1273, 1496, 1565, 1579, 1587, 1755, 1854
RsaNI	GTAC	8 cut(s)	509, 1272, 1495, 1564, 1578, 1586, 1754, 1853
RseI	CAYNNNNRTG	2 cut(s)	588, 1593
SacI	GAGCTC	1 cut(s)	829
SaqAI	TTAA	5 cut(s)	887, 1059, 1380, 1446, 1694
SatI	GCNGC	7 cut(s)	311, 339, 634, 684, 1242, 1680, 1683
Sau3AI	GATC	7 cut(s)	580, 964, 1235, 1291, 1300, 1604, 1661
Sau96I	GGNCC	3 cut(s)	526, 1391, 1781
SchI	GAGTC	3 cut(s)	404, 898, 1222
ScrFI	CCNGG	5 cut(s)	476, 881, 1023, 1455, 1797
SduI	GDGCHC	5 cut(s)	322, 829, 922, 977, 1830
SfaNI	GCATC	4 cut(s)	367, 705, 1324, 1363
SfcI	CTRYAG	2 cut(s)	960, 1680
Sfr274I	CTCGAG	1 cut(s)	602
SinI	GGWCC	3 cut(s)	526, 1391, 1781
SlaI	CTCGAG	1 cut(s)	602
SmiMI	CAYNNNNRTG	2 cut(s)	588, 1593
SmlI	CTYRAG	4 cut(s)	602, 830, 1674, 1808
SmoI	CTYRAG	4 cut(s)	602, 830, 1674, 1808
SnaBI	TACGTA	2 cut(s)	572, 1856
SpeI	ACTAGT	1 cut(s)	1118
SphI	GCATGC	1 cut(s)	310
Sse9I	AATT	4 cut(s)	432, 1053, 1085, 1442
SsiI	CCGC	5 cut(s)	144, 311, 713, 747, 992
SspMI	CTAG	6 cut(s)	294, 611, 680, 1119, 1551, 1593
SstI	GAGCTC	1 cut(s)	829
StyD4I	CCNGG	5 cut(s)	474, 879, 1021, 1453, 1795
StyI	CCWWGG	1 cut(s)	1425
TaaI	ACNGT	4 cut(s)	286, 761, 1516, 1723
TaiI	ACGT	5 cut(s)	210, 574, 694, 1176, 1858
TaqI	TCGA	4 cut(s)	17, 400, 603, 651
TasI	AATT	4 cut(s)	432, 1053, 1085, 1442
TatI	WGTACW	3 cut(s)	1271, 1494, 1577
TauI	GCSGC	1 cut(s)	313
TfiI	GAWTC	4 cut(s)	513, 1397, 1736, 1767
Tru1I	TTAA	5 cut(s)	887, 1059, 1380, 1446, 1694
Tru9I	TTAA	5 cut(s)	887, 1059, 1380, 1446, 1694
TscAI	CASTG	3 cut(s)	177, 261, 460
TseFI	GTSAC	5 cut(s)	130, 613, 901, 1487, 1500
TseI	GCWGC	6 cut(s)	338, 633, 683, 1241, 1679, 1682
Tsp45I	GTSAC	5 cut(s)	130, 613, 901, 1487, 1500
TspDTI	ATGAA	2 cut(s)	246, 611
TspRI	CASTG	3 cut(s)	177, 261, 460
Tth111I	GACNNNGTC	1 cut(s)	128
VpaK11BI	GGWCC	3 cut(s)	526, 1391, 1781
XapI	RAATTY	3 cut(s)	1053, 1085, 1442
XceI	RCATGY	3 cut(s)	310, 1501, 1819
XhoI	CTCGAG	1 cut(s)	602
XmiI	GTMKAC	2 cut(s)	459, 574
XmnI	GAANNNNTTC	2 cut(s)	537, 1411
XspI	CTAG	6 cut(s)	294, 611, 680, 1119, 1551, 1593
ZraI	GACGTC	1 cut(s)	1174
Zsp2I	ATGCAT	2 cut(s)	1050, 1378

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.