Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0012749

Physical Location & Seq

Genomic Coordinates Forward (+)

30939654 .. 30942230

Gene Structure 2577 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1244300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 651 bp

ATGGCGAGAAGGCCGGACGAGGAATACGATTACTTGTTCAAAGTGGTACTAATCGGCGATTCTGGCGTTGGGAAATCGAACCTCCTCTCCCGATTCACTCGCAACGAGTTCTGTTTGGAGTCTAAGTCCACCATTGGCGTCGAATTTGCCACCAGGACTCTCCAGGTTGAGGGGAGAACTGTGAAAGCTCAGATATGGGACACAGCAGGACAGGAGCGATATAGAGCAATTACTAGCGCCTACTATAGAGGCGCCCTTGGAGCTTTACTTGTCTATGATGTAACAAAACCAACAACATTTGAGAACGTAAGCAGGTGGCTGAAGGAATTGAGAGACCATGCTGACGCAAACATTGTGATCATGATGATCGGGAACAAGACTGATCTGAAGCATCTACGTGCAGTTGCCACTGAAGATGCCCAGGGTTATGCCGAAAGGGAAGGCCTTTCATTCATTGAAACGTCCGCTCTTGAAGCAATCAACGTGGAGAAGGCTTTCCAGACAATTCTGGCAGAGATATACCGGATCATTAGTAAGAAGTCTCTCTCATCAGGCGAGCCAACAGCTGCAAGCGTCAAAGAAGGGAAGACTATTGCAGTCACTGGAGGATCAGAGGTCAATACAAAGAAGACTTGCTGTTCTTCAGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005829 GO:0005886 GO:0007049 GO:0008150 GO:0009504 GO:0009987 GO:0012505 GO:0016020 GO:0022402 GO:0031410 GO:0031982 GO:0032506 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0051301 GO:0061640 GO:0071944 GO:0097708 GO:1902410 GO:1903047

KEGG Pathways

Metabolic & Signaling

ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972

Pfam Domains

Protein Families

Ras

Protein Analysis

217

Amino Acids

23.85

Weight (kDa)

7.66

Isoelectric Point (pI)

32.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	13 - 132	2.3e-12	ADP-ribosylation factor family
Ras	PF00071	14 - 174	1.2e-62	Ras family
Roc	PF08477	14 - 128	2.4e-34	Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1	PF01926	14 - 120	3.3e-06	50S ribosome-binding GTPase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012749)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09630
fragaria_vesca	FvH4_5g28090
malus_domestica	MD08G1244300.v1.1 MD15G1439000.v1.1
prunus_persica	Prupe.1G581700_v2.0.a1
pyrus_communis	pycom08g21190 pycom15g38710
rosa_chinensis	RchiOBHm_Chr7g0220381
rosa_laevigata	RLG00000002224
rosa_multiflora	Rmu_sc0000099.1_g000015
rosa_roxburghii	Rroxscaffold_3G00239440
rosa_rugosa	Rorug07G0193400 Rorug07G0193500
rosa_samantha	Rh7AG334700 Rh7BG325600 Rh7CG352400 Rh7DG331500
rosa_wichuraiana	Rw7G028520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	303
Acc36I	ACCTGC	1 cut(s)	303
AccB1I	GGYRCC	1 cut(s)	251
AccBSI	CCGCTC	1 cut(s)	467
AciI	CCGC	1 cut(s)	465
AclWI	GGATC	2 cut(s)	533, 616
AcsI	RAATTY	1 cut(s)	143
AcuI	CTGAAG	4 cut(s)	341, 407, 432, 627
AcyI	GRCGYC	2 cut(s)	138, 252
AfaI	GTAC	1 cut(s)	48
AfiI	CCNNNNNNNGG	1 cut(s)	169
AgsI	TTSAA	3 cut(s)	40, 458, 473
AjnI	CCWGG	3 cut(s)	152, 162, 420
AloI	GAACNNNNNNTCC	2 cut(s)	71, 103
AluBI	AGCT	3 cut(s)	188, 263, 566
AluI	AGCT	3 cut(s)	188, 263, 566
Alw26I	GTCTC	2 cut(s)	327, 546
AlwI	GGATC	2 cut(s)	533, 616
AlwNI	CAGNNNCTG	1 cut(s)	602
AoxI	GGCC	2 cut(s)	11, 442
ApeKI	GCWGC	1 cut(s)	566
ApoI	RAATTY	1 cut(s)	143
Asp700I	GAANNNNTTC	1 cut(s)	494
AspLEI	GCGC	2 cut(s)	239, 254
BanI	GGYRCC	1 cut(s)	251
BbsI	GAAGAC	2 cut(s)	593, 635
BbvI	GCAGC	1 cut(s)	553
BciT130I	CCWGG	3 cut(s)	154, 164, 422
BclI	TGATCA	1 cut(s)	357
BcoDI	GTCTC	2 cut(s)	327, 546
BfaI	CTAG	1 cut(s)	234
BfmI	CTRYAG	1 cut(s)	244
BfoI	RGCGCY	2 cut(s)	240, 255
BfuAI	ACCTGC	1 cut(s)	303
BisI	GCNGC	1 cut(s)	567
BlsI	GCNGC	1 cut(s)	568
Bme1390I	CCNGG	3 cut(s)	154, 164, 422
BmiI	GGNNCC	1 cut(s)	253
BmrFI	CCNGG	3 cut(s)	154, 164, 422
BmsI	GCATC	2 cut(s)	400, 406
BpiI	GAAGAC	2 cut(s)	593, 635
BpmI	CTGGAG	2 cut(s)	146, 624
BsaAI	YACGTR	1 cut(s)	398
BsaHI	GRCGYC	2 cut(s)	138, 252
BsaI	GGTCTC	1 cut(s)	327
BsaJI	CCNNGG	3 cut(s)	256, 420, 421
BsaWI	WCCGGW	1 cut(s)	522
BsaXI	ACNNNNNCTCC	4 cut(s)	71, 101, 110, 140
Bsc4I	CCNNNNNNNGG	1 cut(s)	169
Bse1I	ACTGG	1 cut(s)	607
BseBI	CCWGG	3 cut(s)	154, 164, 422
BseDI	CCNNGG	3 cut(s)	256, 420, 421
BseLI	CCNNNNNNNGG	1 cut(s)	169
BseMII	CTCAG	1 cut(s)	203
BseNI	ACTGG	1 cut(s)	607
BseRI	GAGGAG	1 cut(s)	74
BseXI	GCAGC	1 cut(s)	553
BsgI	GTGCAG	1 cut(s)	420
BshFI	GGCC	2 cut(s)	13, 444
BshNI	GGYRCC	1 cut(s)	251
BsiSI	CCGG	2 cut(s)	14, 523
BslFI	GGGAC	1 cut(s)	212
BslI	CCNNNNNNNGG	1 cut(s)	169
BsmAI	GTCTC	2 cut(s)	327, 546
BsmFI	GGGAC	1 cut(s)	212
BsnI	GGCC	2 cut(s)	13, 444
Bso31I	GGTCTC	1 cut(s)	327
Bsp143I	GATC	5 cut(s)	357, 366, 382, 525, 608
BspACI	CCGC	1 cut(s)	465
BspANI	GGCC	2 cut(s)	13, 444
BspCNI	CTCAG	1 cut(s)	202
BspHI	TCATGA	1 cut(s)	360
BspLI	GGNNCC	1 cut(s)	253
BspMI	ACCTGC	1 cut(s)	303
BspPI	GGATC	2 cut(s)	533, 616
BspT107I	GGYRCC	1 cut(s)	251
BspTNI	GGTCTC	1 cut(s)	327
BsrBI	CCGCTC	1 cut(s)	467
BsrI	ACTGG	1 cut(s)	607
BssECI	CCNNGG	3 cut(s)	256, 420, 421
BssMI	GATC	5 cut(s)	357, 366, 382, 525, 608
BssNI	GRCGYC	2 cut(s)	138, 252
BssT1I	CCWWGG	1 cut(s)	256
Bst2UI	CCWGG	3 cut(s)	154, 164, 422
Bst4CI	ACNGT	1 cut(s)	181
BstACI	GRCGYC	2 cut(s)	138, 252
BstBAI	YACGTR	1 cut(s)	398
BstC8I	GCNNGC	2 cut(s)	557, 571
BstDEI	CTNAG	2 cut(s)	123, 189
BstH2I	RGCGCY	2 cut(s)	240, 255
BstHHI	GCGC	2 cut(s)	239, 254
BstKTI	GATC	5 cut(s)	360, 369, 385, 528, 611
BstMAI	GTCTC	2 cut(s)	327, 546
BstMBI	GATC	5 cut(s)	357, 366, 382, 525, 608
BstMWI	GCNNNNNNNGC	3 cut(s)	63, 260, 473
BstNI	CCWGG	3 cut(s)	154, 164, 422
BstSCI	CCNGG	3 cut(s)	152, 162, 420
BstSFI	CTRYAG	1 cut(s)	244
BstV1I	GCAGC	1 cut(s)	553
BstV2I	GAAGAC	2 cut(s)	593, 635
BsuRI	GGCC	2 cut(s)	13, 444
BtsIMutI	CAGTG	2 cut(s)	408, 600
BveI	ACCTGC	1 cut(s)	303
Cac8I	GCNNGC	2 cut(s)	557, 571
CaiI	CAGNNNCTG	1 cut(s)	602
CciI	TCATGA	1 cut(s)	360
CfoI	GCGC	2 cut(s)	239, 254
CseI	GACGC	3 cut(s)	127, 353, 562
Csp6I	GTAC	1 cut(s)	47
CspCI	CAANNNNNGTGG	2 cut(s)	465, 500
CviAII	CATG	2 cut(s)	338, 361
CviJI	RGCY	9 cut(s)	13, 188, 263, 319, 444, 494, 559, 566, 647
CviKI_1	RGCY	9 cut(s)	13, 188, 263, 319, 444, 494, 559, 566, 647
CviQI	GTAC	1 cut(s)	47
DdeI	CTNAG	2 cut(s)	123, 189
DinI	GGCGCC	1 cut(s)	253
DpnI	GATC	5 cut(s)	359, 368, 384, 527, 610
DpnII	GATC	5 cut(s)	357, 366, 382, 525, 608
Eco130I	CCWWGG	1 cut(s)	256
Eco147I	AGGCCT	1 cut(s)	444
Eco31I	GGTCTC	1 cut(s)	327
Eco57I	CTGAAG	4 cut(s)	341, 407, 432, 627
EcoRII	CCWGG	3 cut(s)	152, 162, 420
EcoT14I	CCWWGG	1 cut(s)	256
EgeI	GGCGCC	1 cut(s)	253
EheI	GGCGCC	1 cut(s)	253
ErhI	CCWWGG	1 cut(s)	256
FaeI	CATG	2 cut(s)	341, 364
FaiI	YATR	8 cut(s)	196, 222, 246, 276, 339, 362, 429, 520
FaqI	GGGAC	1 cut(s)	212
FatI	CATG	2 cut(s)	337, 360
FbaI	TGATCA	1 cut(s)	357
Fnu4HI	GCNGC	1 cut(s)	567
Fsp4HI	GCNGC	1 cut(s)	567
FspBI	CTAG	1 cut(s)	234
GlaI	GCGC	2 cut(s)	238, 253
GluI	GCNGC	1 cut(s)	567
GsuI	CTGGAG	2 cut(s)	146, 624
HaeII	RGCGCY	2 cut(s)	240, 255
HaeIII	GGCC	2 cut(s)	13, 444
HapII	CCGG	2 cut(s)	14, 523
HgaI	GACGC	3 cut(s)	127, 353, 562
HhaI	GCGC	2 cut(s)	239, 254
Hin1I	GRCGYC	2 cut(s)	138, 252
Hin1II	CATG	2 cut(s)	341, 364
Hin6I	GCGC	2 cut(s)	237, 252
HinP1I	GCGC	2 cut(s)	237, 252
HinfI	GANTC	4 cut(s)	59, 93, 119, 157
HpaII	CCGG	2 cut(s)	14, 523
Hpy166II	GTNNAC	1 cut(s)	129
Hpy188I	TCNGA	3 cut(s)	192, 387, 613
Hpy188III	TCNNGA	5 cut(s)	90, 361, 370, 470, 499
Hpy8I	GTNNAC	1 cut(s)	129
Hpy99I	CGWCG	1 cut(s)	143
HpyAV	CCTTC	5 cut(s)	3, 316, 434, 484, 575
HpyCH4III	ACNGT	1 cut(s)	181
HpyCH4IV	ACGT	4 cut(s)	306, 397, 461, 483
HpyCH4V	TGCA	3 cut(s)	401, 569, 596
HpyF10VI	GCNNNNNNNGC	3 cut(s)	63, 260, 473
HpyF3I	CTNAG	2 cut(s)	123, 189
HpySE526I	ACGT	4 cut(s)	306, 397, 461, 483
Hsp92I	GRCGYC	2 cut(s)	138, 252
Hsp92II	CATG	2 cut(s)	341, 364
HspAI	GCGC	2 cut(s)	237, 252
KasI	GGCGCC	1 cut(s)	251
Ksp22I	TGATCA	1 cut(s)	357
Kzo9I	GATC	5 cut(s)	357, 366, 382, 525, 608
LmnI	GCTCC	2 cut(s)	214, 260
Lsp1109I	GCAGC	1 cut(s)	553
LweI	GCATC	2 cut(s)	400, 406
MaeI	CTAG	1 cut(s)	234
MaeII	ACGT	4 cut(s)	306, 397, 461, 483
MaeIII	GTNAC	2 cut(s)	280, 598
MalI	GATC	5 cut(s)	359, 368, 384, 527, 610
MbiI	CCGCTC	1 cut(s)	467
MboI	GATC	5 cut(s)	357, 366, 382, 525, 608
MboII	GAAGA	4 cut(s)	425, 598, 633, 640
MluCI	AATT	4 cut(s)	143, 228, 326, 504
Mly113I	GGCGCC	1 cut(s)	252
MlyI	GAGTC	2 cut(s)	128, 151
MnlI	CCTC	7 cut(s)	13, 92, 95, 163, 242, 599, 607
MroXI	GAANNNNTTC	1 cut(s)	494
MslI	CAYNNNNRTG	1 cut(s)	396
MspA1I	CMGCKG	1 cut(s)	566
MspI	CCGG	2 cut(s)	14, 523
MspR9I	CCNGG	3 cut(s)	154, 164, 422
MvaI	CCWGG	3 cut(s)	154, 164, 422
MwoI	GCNNNNNNNGC	3 cut(s)	63, 260, 473
NarI	GGCGCC	1 cut(s)	252
NdeII	GATC	5 cut(s)	357, 366, 382, 525, 608
NlaIII	CATG	2 cut(s)	341, 364
NlaIV	GGNNCC	1 cut(s)	253
NmuCI	GTSAC	1 cut(s)	598
PagI	TCATGA	1 cut(s)	360
PaqCI	CACCTGC	1 cut(s)	303
PasI	CCCWGGG	1 cut(s)	421
PceI	AGGCCT	1 cut(s)	444
PcsI	WCGNNNNNNNCGW	1 cut(s)	24
PdmI	GAANNNNTTC	1 cut(s)	494
PfeI	GAWTC	2 cut(s)	59, 93
PkrI	GCNGC	1 cut(s)	568
PleI	GAGTC	2 cut(s)	127, 151
PluTI	GGCGCC	1 cut(s)	255
PpsI	GAGTC	2 cut(s)	127, 151
Ppu21I	YACGTR	1 cut(s)	398
Psp6I	CCWGG	3 cut(s)	152, 162, 420
PspGI	CCWGG	3 cut(s)	152, 162, 420
PspN4I	GGNNCC	1 cut(s)	253
PstNI	CAGNNNCTG	1 cut(s)	602
PvuII	CAGCTG	1 cut(s)	566
RsaI	GTAC	1 cut(s)	48
RsaNI	GTAC	1 cut(s)	47
RseI	CAYNNNNRTG	1 cut(s)	396
SatI	GCNGC	1 cut(s)	567
Sau3AI	GATC	5 cut(s)	357, 366, 382, 525, 608
SchI	GAGTC	2 cut(s)	128, 151
ScrFI	CCNGG	3 cut(s)	154, 164, 422
SfaNI	GCATC	2 cut(s)	400, 406
SfcI	CTRYAG	1 cut(s)	244
SfoI	GGCGCC	1 cut(s)	253
SmiMI	CAYNNNNRTG	1 cut(s)	396
Sse9I	AATT	4 cut(s)	143, 228, 326, 504
SseBI	AGGCCT	1 cut(s)	444
SsiI	CCGC	1 cut(s)	465
SspDI	GGCGCC	1 cut(s)	251
SspMI	CTAG	1 cut(s)	234
StuI	AGGCCT	1 cut(s)	444
StyD4I	CCNGG	3 cut(s)	152, 162, 420
StyI	CCWWGG	1 cut(s)	256
TaaI	ACNGT	1 cut(s)	181
TaiI	ACGT	4 cut(s)	309, 400, 464, 486
TaqI	TCGA	2 cut(s)	77, 141
TasI	AATT	4 cut(s)	143, 228, 326, 504
TfiI	GAWTC	2 cut(s)	59, 93
TscAI	CASTG	2 cut(s)	415, 607
TseFI	GTSAC	1 cut(s)	598
TseI	GCWGC	1 cut(s)	566
Tsp45I	GTSAC	1 cut(s)	598
TspDTI	ATGAA	2 cut(s)	438, 442
TspRI	CASTG	2 cut(s)	415, 607
XapI	RAATTY	1 cut(s)	143
XmnI	GAANNNNTTC	1 cut(s)	494
XspI	CTAG	1 cut(s)	234

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.