MD09G1007300.v1.1

May serve as docking site to facilitate the association of other proteins to the plasma membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014969
Physical Location & Seq
Reverse (-)
514577 .. 518001
3425 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1007300.v1.1.491

Sequence Viewer

Length: 357 bp
ATGCCGGAGGAAGACCTGGTGGAGCTCAAGTTCCGTCTGTATGATGGATCGGATATCGGCCCGTTCCGGTACTCGCCGGCGTCCACGGTGGCAATGCTCAAGGAGAGAATCGTGGCCGAGTGGCCAAAAGATAAAAAGATTGCACCAAAAGGAGCAAACGACATAAAATTGATAAATGCTGGGAAGATTTTGGAAAACAACAAGACTGTTGCCCAGTGTAGAGCACCATTTGGAGAGCTTCCAAGCGGGATTATCACCATGCATGTTGTTGTTCAGCCCTCTATAGCGAAAGCAAAAACAGAGAAGAAGGTTGACGAGGTCCCAAGGAAAAGCATGTGTTCTTGTTCGATATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rad60-SLD_2

Protein Analysis

119

Amino Acids

13.04

Weight (kDa)

9.01

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD_2 PF13881 5 - 116 9.3e-44 Ubiquitin-2 like Rad60 SUMO-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014969)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26980
fragaria_vesca FvH4_6g53910
malus_domestica MD09G1007300.v1.1 MD17G1011200.v1.1
prunus_persica Prupe.3G309700_v2.0.a1
pyrus_communis pycom111g00560 pycom17g00590
rosa_chinensis RchiOBHm_Chr2g0176071
rosa_laevigata RLG00000022378
rosa_roxburghii Rroxscaffold_2G00076790
rosa_rugosa Rorug02G0590200
rosa_samantha Rh2AG669000 Rh2BG680600 Rh2CG643500 Rh2DG694300
rosa_wichuraiana Rw2G054870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 246
AclWI GGATC 1 cut(s) 55
AcoI YGGCCR 2 cut(s) 114, 122
AcyI GRCGYC 1 cut(s) 80
AfaI GTAC 1 cut(s) 71
AjnI CCWGG 1 cut(s) 15
AloI GAACNNNNNNTCC 2 cut(s) 14, 46
AluBI AGCT 2 cut(s) 25, 238
AluI AGCT 2 cut(s) 25, 238
Alw21I GWGCWC 2 cut(s) 27, 226
AlwI GGATC 1 cut(s) 55
AoxI GGCC 3 cut(s) 58, 114, 122
AspS9I GGNCC 2 cut(s) 59, 319
AsuHPI GGTGA 1 cut(s) 247
AvaII GGWCC 1 cut(s) 319
BalI TGGCCA 1 cut(s) 124
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 2 cut(s) 27, 226
BccI CCATC 1 cut(s) 38
BciT130I CCWGG 1 cut(s) 17
BfmI CTRYAG 1 cut(s) 282
Bme1390I CCNGG 1 cut(s) 17
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 59, 319
BmiI GGNNCC 1 cut(s) 321
BmrFI CCNGG 1 cut(s) 17
BmrI ACTGGG 1 cut(s) 208
BmuI ACTGGG 1 cut(s) 208
BpiI GAAGAC 1 cut(s) 18
BpuEI CTTGAG 2 cut(s) 11, 83
BsaHI GRCGYC 1 cut(s) 80
BsaJI CCNNGG 2 cut(s) 84, 323
BsaWI WCCGGW 1 cut(s) 66
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 17
BseDI CCNNGG 2 cut(s) 84, 323
BseMI GCAATG 1 cut(s) 99
BseNI ACTGG 1 cut(s) 214
BseYI CCCAGC 1 cut(s) 179
BshFI GGCC 3 cut(s) 60, 116, 124
BsiHKAI GWGCWC 2 cut(s) 27, 226
BsiSI CCGG 3 cut(s) 5, 67, 77
BslFI GGGAC 1 cut(s) 305
BsmFI GGGAC 1 cut(s) 305
BsnI GGCC 3 cut(s) 60, 116, 124
Bsp1286I GDGCHC 2 cut(s) 27, 226
Bsp143I GATC 1 cut(s) 47
BspACI CCGC 1 cut(s) 246
BspANI GGCC 3 cut(s) 60, 116, 124
BspLI GGNNCC 1 cut(s) 321
BspPI GGATC 1 cut(s) 55
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 1 cut(s) 214
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 2 cut(s) 84, 323
BssMI GATC 1 cut(s) 47
BssNI GRCGYC 1 cut(s) 80
BssT1I CCWWGG 1 cut(s) 323
Bst2UI CCWGG 1 cut(s) 17
Bst4CI ACNGT 2 cut(s) 88, 208
BstACI GRCGYC 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 78
BstDSI CCRYGG 1 cut(s) 84
BstKTI GATC 1 cut(s) 50
BstMBI GATC 1 cut(s) 47
BstNI CCWGG 1 cut(s) 17
BstNSI RCATGY 2 cut(s) 266, 337
BstSCI CCNGG 1 cut(s) 15
BstSFI CTRYAG 1 cut(s) 282
BstV2I GAAGAC 1 cut(s) 18
BsuRI GGCC 3 cut(s) 60, 116, 124
BtgI CCRYGG 1 cut(s) 84
BtsIMutI CAGTG 1 cut(s) 221
Cac8I GCNNGC 1 cut(s) 78
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 59, 319
CseI GACGC 1 cut(s) 69
CsiI ACCWGGT 1 cut(s) 15
Csp6I GTAC 1 cut(s) 70
CviAII CATG 3 cut(s) 259, 263, 334
CviJI RGCY 6 cut(s) 25, 60, 116, 124, 238, 277
CviKI_1 RGCY 6 cut(s) 25, 60, 116, 124, 238, 277
CviQI GTAC 1 cut(s) 70
DpnI GATC 1 cut(s) 49
DpnII GATC 1 cut(s) 47
EaeI YGGCCR 2 cut(s) 114, 122
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 323
Eco24I GRGCYC 1 cut(s) 27
Eco32I GATATC 1 cut(s) 55
Eco47I GGWCC 1 cut(s) 319
Eco53kI GAGCTC 1 cut(s) 25
EcoICRI GAGCTC 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 15
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 323
EcoT22I ATGCAT 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 323
FaeI CATG 3 cut(s) 262, 266, 337
FaiI YATR 6 cut(s) 42, 164, 260, 264, 284, 335
FaqI GGGAC 1 cut(s) 305
FatI CATG 3 cut(s) 258, 262, 333
FauI CCCGC 1 cut(s) 239
FriOI GRGCYC 1 cut(s) 27
GsaI CCCAGC 1 cut(s) 183
HaeIII GGCC 3 cut(s) 60, 116, 124
HapII CCGG 3 cut(s) 5, 67, 77
HgaI GACGC 1 cut(s) 69
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 3 cut(s) 262, 266, 337
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 1 cut(s) 108
HpaII CCGG 3 cut(s) 5, 67, 77
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 2 cut(s) 84, 313
Hpy188I TCNGA 1 cut(s) 52
Hpy8I GTNNAC 2 cut(s) 84, 313
HpyAV CCTTC 1 cut(s) 301
HpyCH4III ACNGT 2 cut(s) 88, 208
HpyCH4V TGCA 2 cut(s) 143, 262
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 3 cut(s) 262, 266, 337
KroI GCCGGC 1 cut(s) 76
KroNI GCCGGC 1 cut(s) 78
Kzo9I GATC 1 cut(s) 47
LmnI GCTCC 2 cut(s) 22, 152
LpnPI CCDG 7 cut(s) 2, 18, 29, 80, 90, 165, 227
MabI ACCWGGT 1 cut(s) 15
MalI GATC 1 cut(s) 49
MboI GATC 1 cut(s) 47
MboII GAAGA 3 cut(s) 23, 196, 316
MhlI GDGCHC 2 cut(s) 27, 226
MlsI TGGCCA 1 cut(s) 124
MluCI AATT 1 cut(s) 167
MluNI TGGCCA 1 cut(s) 124
MnlI CCTC 2 cut(s) 289, 310
Mox20I TGGCCA 1 cut(s) 124
Mph1103I ATGCAT 1 cut(s) 264
MreI CGCCGGCG 1 cut(s) 76
MroNI GCCGGC 1 cut(s) 76
MscI TGGCCA 1 cut(s) 124
Msp20I TGGCCA 1 cut(s) 124
MspI CCGG 3 cut(s) 5, 67, 77
MspR9I CCNGG 1 cut(s) 17
MvaI CCWGG 1 cut(s) 17
NaeI GCCGGC 1 cut(s) 78
NdeII GATC 1 cut(s) 47
NgoMIV GCCGGC 1 cut(s) 76
NlaIII CATG 3 cut(s) 262, 266, 337
NlaIV GGNNCC 1 cut(s) 321
NmeAIII GCCGAG 1 cut(s) 142
NsiI ATGCAT 1 cut(s) 264
NspI RCATGY 2 cut(s) 266, 337
PdiI GCCGGC 1 cut(s) 78
PfeI GAWTC 1 cut(s) 108
PflFI GACNNNGTC 1 cut(s) 317
PpuMI RGGWCCY 1 cut(s) 319
Psp124BI GAGCTC 1 cut(s) 27
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 15
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 321
PspPI GGNCC 2 cut(s) 59, 319
PspPPI RGGWCCY 1 cut(s) 319
PsyI GACNNNGTC 1 cut(s) 317
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
SacI GAGCTC 1 cut(s) 27
Sau3AI GATC 1 cut(s) 47
Sau96I GGNCC 2 cut(s) 59, 319
ScrFI CCNGG 1 cut(s) 17
SduI GDGCHC 2 cut(s) 27, 226
SetI ASST 5 cut(s) 18, 27, 240, 312, 321
SexAI ACCWGGT 1 cut(s) 15
SfcI CTRYAG 1 cut(s) 282
SgrAI CRCCGGYG 1 cut(s) 76
SinI GGWCC 1 cut(s) 319
SmlI CTYRAG 2 cut(s) 26, 98
SmoI CTYRAG 2 cut(s) 26, 98
Sse9I AATT 1 cut(s) 167
SsiI CCGC 1 cut(s) 246
SstI GAGCTC 1 cut(s) 27
StyD4I CCNGG 1 cut(s) 15
StyI CCWWGG 1 cut(s) 323
TaaI ACNGT 2 cut(s) 88, 208
TaqI TCGA 1 cut(s) 347
TasI AATT 1 cut(s) 167
TfiI GAWTC 1 cut(s) 108
TscAI CASTG 1 cut(s) 221
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 1 cut(s) 221
Tth111I GACNNNGTC 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 319
XceI RCATGY 2 cut(s) 266, 337
Zsp2I ATGCAT 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.