RLG00000022378

May serve as docking site to facilitate the association of other proteins to the plasma membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014969
Physical Location & Seq
Forward (+)
82971454 .. 82973750
2297 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022378

Sequence Viewer

Length: 357 bp
ATGCCGGAGGAGGACCTGGTCGAGCTCAAGTTCCGACTCTATGACGGATCGGATATCGGTCCTTTCCGCTACTCCCCGGCCTCTACAGTGGCAGTGCTTAAGGAGAGAATCGTCACCGAGTGGCCTAAAGATAAAAAAATTTCACCAAAAGGAGCAAACGACATAAAATTGATAAATGCTGGGAAGATCTTGGAAAACAACAAGACTGTTGGCCAGTGTAGAGTACCATTTGGAGAGCTTCCAAGAGGAATTATCACCATGCATGTTGTTGTTCAGCCTTCTGTAGCAAAAGCAAAAACAGAAAAGAAGATTGATGAGGCCCCAAGGAAACACATATGTTCATGTTCAATATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rad60-SLD_2

Protein Analysis

119

Amino Acids

13.14

Weight (kDa)

9.2

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD_2 PF13881 5 - 116 1.2e-43 Ubiquitin-2 like Rad60 SUMO-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014969)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26980
fragaria_vesca FvH4_6g53910
malus_domestica MD09G1007300.v1.1 MD17G1011200.v1.1
prunus_persica Prupe.3G309700_v2.0.a1
pyrus_communis pycom111g00560 pycom17g00590
rosa_chinensis RchiOBHm_Chr2g0176071
rosa_laevigata RLG00000022378
rosa_roxburghii Rroxscaffold_2G00076790
rosa_rugosa Rorug02G0590200
rosa_samantha Rh2AG669000 Rh2BG680600 Rh2CG643500 Rh2DG694300
rosa_wichuraiana Rw2G054870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 67
AclWI GGATC 1 cut(s) 55
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 1 cut(s) 138
AdeI CACNNNGTG 1 cut(s) 120
AfaI GTAC 1 cut(s) 225
AflII CTTAAG 1 cut(s) 98
AgsI TTSAA 1 cut(s) 348
AjnI CCWGG 1 cut(s) 15
AluBI AGCT 2 cut(s) 25, 238
AluI AGCT 2 cut(s) 25, 238
Alw21I GWGCWC 1 cut(s) 27
AlwI GGATC 1 cut(s) 55
AoxI GGCC 4 cut(s) 78, 122, 211, 318
ApoI RAATTY 1 cut(s) 138
AspS9I GGNCC 3 cut(s) 13, 59, 319
AsuC2I CCSGG 1 cut(s) 77
AsuHPI GGTGA 3 cut(s) 106, 135, 247
AvaII GGWCC 2 cut(s) 13, 59
BalI TGGCCA 1 cut(s) 213
BanII GRGCYC 1 cut(s) 27
Bbv12I GWGCWC 1 cut(s) 27
BciT130I CCWGG 1 cut(s) 17
BcnI CCSGG 1 cut(s) 77
BfmI CTRYAG 2 cut(s) 84, 282
BfrI CTTAAG 1 cut(s) 98
BglII AGATCT 1 cut(s) 186
Bme1390I CCNGG 2 cut(s) 17, 77
Bme18I GGWCC 2 cut(s) 13, 59
BmgT120I GGNCC 3 cut(s) 13, 59, 319
BmiI GGNNCC 1 cut(s) 321
BmrFI CCNGG 2 cut(s) 17, 77
BpuEI CTTGAG 1 cut(s) 11
BpuMI CCSGG 1 cut(s) 77
BsaJI CCNNGG 2 cut(s) 75, 323
Bse1I ACTGG 1 cut(s) 214
BseBI CCWGG 1 cut(s) 17
BseDI CCNNGG 2 cut(s) 75, 323
BseNI ACTGG 1 cut(s) 214
BseRI GAGGAG 1 cut(s) 23
BseYI CCCAGC 1 cut(s) 179
BshFI GGCC 4 cut(s) 80, 124, 213, 320
BsiHKAI GWGCWC 1 cut(s) 27
BsiSI CCGG 2 cut(s) 5, 77
BsnI GGCC 4 cut(s) 80, 124, 213, 320
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 2 cut(s) 47, 186
BspACI CCGC 1 cut(s) 67
BspANI GGCC 4 cut(s) 80, 124, 213, 320
BspLI GGNNCC 1 cut(s) 321
BspPI GGATC 1 cut(s) 55
BspTI CTTAAG 1 cut(s) 98
BsrI ACTGG 1 cut(s) 214
BssECI CCNNGG 2 cut(s) 75, 323
BssMI GATC 2 cut(s) 47, 186
BssT1I CCWWGG 1 cut(s) 323
Bst2UI CCWGG 1 cut(s) 17
Bst4CI ACNGT 2 cut(s) 88, 208
BstAFI CTTAAG 1 cut(s) 98
BstKTI GATC 2 cut(s) 50, 189
BstMBI GATC 2 cut(s) 47, 186
BstNI CCWGG 1 cut(s) 17
BstNSI RCATGY 1 cut(s) 266
BstSCI CCNGG 2 cut(s) 15, 75
BstSFI CTRYAG 2 cut(s) 84, 282
BstX2I RGATCY 1 cut(s) 186
BstYI RGATCY 1 cut(s) 186
BsuRI GGCC 4 cut(s) 80, 124, 213, 320
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 3 cut(s) 93, 99, 221
Cfr13I GGNCC 3 cut(s) 13, 59, 319
CsiI ACCWGGT 1 cut(s) 15
Csp6I GTAC 1 cut(s) 224
CviAII CATG 3 cut(s) 259, 263, 342
CviJI RGCY 7 cut(s) 25, 80, 124, 213, 238, 277, 320
CviKI_1 RGCY 7 cut(s) 25, 80, 124, 213, 238, 277, 320
CviQI GTAC 1 cut(s) 224
DpnI GATC 2 cut(s) 49, 188
DpnII GATC 2 cut(s) 47, 186
DraIII CACNNNGTG 1 cut(s) 120
EaeI YGGCCR 1 cut(s) 211
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 323
Eco24I GRGCYC 1 cut(s) 27
Eco32I GATATC 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 13, 59
Eco53kI GAGCTC 1 cut(s) 25
EcoICRI GAGCTC 1 cut(s) 25
EcoO109I RGGNCCY 2 cut(s) 13, 319
EcoRII CCWGG 1 cut(s) 15
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 323
EcoT22I ATGCAT 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 323
FaeI CATG 3 cut(s) 262, 266, 345
FaiI YATR 7 cut(s) 42, 164, 260, 264, 335, 337, 343
FatI CATG 3 cut(s) 258, 262, 341
FauNDI CATATG 1 cut(s) 335
FriOI GRGCYC 1 cut(s) 27
GsaI CCCAGC 1 cut(s) 183
HaeIII GGCC 4 cut(s) 80, 124, 213, 320
HapII CCGG 2 cut(s) 5, 77
Hin1II CATG 3 cut(s) 262, 266, 345
HinfI GANTC 2 cut(s) 36, 108
HpaII CCGG 2 cut(s) 5, 77
HphI GGTGA 3 cut(s) 106, 135, 247
Hpy188I TCNGA 2 cut(s) 35, 52
HpyAV CCTTC 1 cut(s) 288
HpyCH4III ACNGT 2 cut(s) 88, 208
HpyCH4V TGCA 1 cut(s) 262
Hsp92II CATG 3 cut(s) 262, 266, 345
Kzo9I GATC 2 cut(s) 47, 186
LmnI GCTCC 1 cut(s) 152
LpnPI CCDG 6 cut(s) 2, 18, 29, 90, 165, 227
MabI ACCWGGT 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 112
MalI GATC 2 cut(s) 49, 188
MboI GATC 2 cut(s) 47, 186
MboII GAAGA 2 cut(s) 196, 319
MflI RGATCY 1 cut(s) 186
MhlI GDGCHC 1 cut(s) 27
MlsI TGGCCA 1 cut(s) 213
MluCI AATT 3 cut(s) 138, 167, 249
MluNI TGGCCA 1 cut(s) 213
MlyI GAGTC 1 cut(s) 30
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 4 cut(s) 4, 91, 239, 310
Mox20I TGGCCA 1 cut(s) 213
Mph1103I ATGCAT 1 cut(s) 264
MscI TGGCCA 1 cut(s) 213
MseI TTAA 1 cut(s) 99
Msp20I TGGCCA 1 cut(s) 213
MspCI CTTAAG 1 cut(s) 98
MspI CCGG 2 cut(s) 5, 77
MspR9I CCNGG 2 cut(s) 17, 77
MvaI CCWGG 1 cut(s) 17
NciI CCSGG 1 cut(s) 77
NdeI CATATG 1 cut(s) 335
NdeII GATC 2 cut(s) 47, 186
NlaIII CATG 3 cut(s) 262, 266, 345
NlaIV GGNNCC 1 cut(s) 321
NmuCI GTSAC 1 cut(s) 112
NsiI ATGCAT 1 cut(s) 264
NspI RCATGY 1 cut(s) 266
PfeI GAWTC 1 cut(s) 108
PflFI GACNNNGTC 1 cut(s) 17
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
PpuMI RGGWCCY 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 27
Psp5II RGGWCCY 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 15
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 321
PspPI GGNCC 3 cut(s) 13, 59, 319
PspPPI RGGWCCY 1 cut(s) 13
PsuI RGATCY 1 cut(s) 186
PsyI GACNNNGTC 1 cut(s) 17
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
SacI GAGCTC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 99
Sau3AI GATC 2 cut(s) 47, 186
Sau96I GGNCC 3 cut(s) 13, 59, 319
SchI GAGTC 1 cut(s) 30
ScrFI CCNGG 2 cut(s) 17, 77
SduI GDGCHC 1 cut(s) 27
SetI ASST 3 cut(s) 18, 27, 240
SexAI ACCWGGT 1 cut(s) 15
SfcI CTRYAG 2 cut(s) 84, 282
SinI GGWCC 2 cut(s) 13, 59
SmlI CTYRAG 2 cut(s) 26, 98
SmoI CTYRAG 2 cut(s) 26, 98
Sse9I AATT 3 cut(s) 138, 167, 249
SsiI CCGC 1 cut(s) 67
SspI AATATT 1 cut(s) 351
SstI GAGCTC 1 cut(s) 27
StyD4I CCNGG 2 cut(s) 15, 75
StyI CCWWGG 1 cut(s) 323
TaaI ACNGT 2 cut(s) 88, 208
TaqI TCGA 1 cut(s) 21
TaqII GACCGA 1 cut(s) 47
TasI AATT 3 cut(s) 138, 167, 249
TfiI GAWTC 1 cut(s) 108
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TscAI CASTG 3 cut(s) 93, 99, 221
TseFI GTSAC 1 cut(s) 112
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 1 cut(s) 330
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 3 cut(s) 93, 99, 221
Tth111I GACNNNGTC 1 cut(s) 17
Vha464I CTTAAG 1 cut(s) 98
VpaK11BI GGWCC 2 cut(s) 13, 59
XapI RAATTY 1 cut(s) 138
XceI RCATGY 1 cut(s) 266
Zsp2I ATGCAT 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.