Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0011810

Physical Location & Seq

Genomic Coordinates Forward (+)

2216585 .. 2221974

Gene Structure 5390 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1035900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2328 bp

ATGGATCTGGAGGGGGTGAGCCGCAGCCATCCAATCAAGAAGGATTCATGGAAGACGCTGTTAACTCTGGCTTACCAGAGCCTGGGAGTAGTATATGGAGATTTAAGCACTTCTCCTCTCTATGTTTACAAAAGCACTTTTGCAGAAGATATTCACCACTCAGAAACCAATGAGGAGATTTTTGGGGTTCTCTCGTTTGTTTTCTGGACATTGACGCTGATTCCATTAGTGAAATATGTGTTTATTGTGCTTAGAGCCGACGACAATGGCGAAGGTGGGACTTTTGCTCTGTATTCTCTGCTTTCCCGGCACGCCAGGGTGAGCTCCTTGCCCAATTGCCAGCTGGCAGATGAGGAGCTATCGGAGTACACCAAAGATGGGGTTGTTCCGACGGCCAACAGTGCTTTCGGGTCGAGTTTGAAATCGACATTGGAGAAGCATAAGGTGCTGAAAAAGGTGCTGCTTGTTCTGGCTTTGATTGGAACTTGTATGGTTATTGGTGATGGGGTGCTCACACCAGCAATTTCTGTTTTTTCCGCTGTTTCGGGGCTGGAGCTCTCCATGTCGAAACAGCAGCATCGATATGTTGAAGTCCCGGTTGCTTGTGTCATACTCATATTTCTGTTTGCCCTTCAACATTACGGGACCCACAGAGTAGGGTTCTTGTTTGCACCAATTGTGATCACATGGCTTTTGTGCATCAGTTCCATTGGTGTATACAACATCTTCCATTGGAATCCCCATGTTTATCAAGCACTCTCTCCATACTACATGTACAAATTTTTGAAGAAGACCCGAAAGGGAGGTTGGATGTCCTTGGGCGGTATACTATTGTGTATGACAGGCTCGGAAGCTATGTTTGCTGATCTCGGACACTTTTCACAGACGTCAATCAAGATTGCTTTCACCTTTGTGGTTTACCCGTCACTGATTCTAGCATATATGGGACAAGCTGCATACCTTTCTAGGCATCATATCGTAGCAAGTGATTATCGGATTGGCTTCTATGAATCAGTTCCTGAGAAAATAAGATGGCCGGTTTTGGCAATAGCCATACTTGCTGCGGTGGTGGGAAGTCAAGCCATTATAACTGGAACTTTCTCGATAATCAAACAATGTTCTGCTTTGGGTTGCTTTCCAAGGGTCAAAATTATCCACACGTCTTCGAAAATACATGGTCAAATTTACATCCCCGAGATTAACTGGACTTTGATGTTGCTATGCTTGGCTGTTACCATTGGTTTTAGAGACACAAAATCCATGGGAAATGCATCAGGTTTGGCCGTCATAACTGTCATGCTGGTAACTACCTGCCTTATGTCTCTAGTCATAGTCTTGTGCTGGCACAAAAGCCTCTTCTGGGCAGTTTGCTTTATATTATTCTTCGGCTCCCTTGAGGCACTCTACTTCTCAGCATCGCTCATAAAGTTTCGTGAAGGGGCTTGGGTCCCGGTTGCTCTTTCATTCATCTTCTTAATGGTTATGTATGTTTGGCAATACGGCACCATCAAGAAGTACGAGTTTGATGTCCAAAATAAAGTCTCCATCAACTGGCTCCTCAGTTTAGGTCCTACTCTAGGGATTGTTCGAGTCCGGGGAATTGGCCTTATACACACTGAGCTTGTCTCTGGGATCCCGGCTATTTTCTCTCACTTTGTTACCAACCTCCCTGCTTTCCACCAGGTTGTAGTTTTCCTCTGCATCAAATCCGTCCCAGTCCCACATGTCAGACCCGAGGAAAGATTCTTAGTGGGAAGAGTTGGTCCAAAGGAATACCGGCTATATAGATGCATAGCACGCTATGGTTACCGTGATGTTCACAAGGATGACATGGAATTCGAAAGAGATCTAGTTTGCAGTATTGCAGAGTTCATTCGGTCAGAGAGACCAGAATGCAATCTCAGTCCAGAACAACTGGAAGACGATGAGAAAATGACAGTTGTTGGAACTTCATCATCAAACTTAGATGGCATAAGAATGTCTGTAGACGAGGCAGATTTTTCCGAAATAGCAAGCACCTCGGAGTTGCAGGAGATAAGACCTACAGAAAGGGCAAAGAAGAGAGTGAGGTTTGTCGTTCCAGAAACCCCACGAATCGATAGGGAAGCAGTAGAGGAGCTGCAGGAACTGATGGAAGCAAGGGAGGCAGGAATGGCATTTATATTAGGCCACTCGTACGTGAAGGCGAAGAGAGGTTCAAGTTTTATGAAGAAAATAGTGATCAATTTCGGGTACGATTTCTTGAGGAGGAATTTTCGAGGGCCGAGTTATGCTTTGAGCATTCCTCATGCTTCTACTCTAGAGGTTGGGATGGTCTACCACGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

776

Amino Acids

87.03

Weight (kDa)

8.44

Isoelectric Point (pI)

38.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	23 - 520	6.6e-171	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	533 - 774	2.1e-27	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011810)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14880
fragaria_vesca	FvH4_6g50500 FvH4_6g50500
malus_domestica	MD09G1035900.v1.1 MD17G1037500.v1.1
prunus_persica	Prupe.3G281900_v2.0.a1 Prupe.3G281900_v2.0.a1
pyrus_communis	pycom111g02870 pycom17g03360
rosa_chinensis	RchiOBHm_Chr2g0170921
rosa_laevigata	RLG00000021992
rosa_multiflora	Rmu_sc0009938.1_g000001
rosa_roxburghii	Rroxscaffold_2G00080980
rosa_rugosa	Rorug02G0552300
rosa_samantha	Rh2AG625000 Rh2BG635700 Rh2CG604400 Rh2DG647300
rosa_wichuraiana	Rw2G051770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1088
AatII	GACGTC	1 cut(s)	890
Acc36I	ACCTGC	1 cut(s)	1319
AccB1I	GGYRCC	1 cut(s)	1502
AccB7I	CCANNNNNTGG	2 cut(s)	82, 1551
AccI	GTMKAC	4 cut(s)	717, 826, 1986, 2316
AciI	CCGC	4 cut(s)	22, 537, 822, 1064
AclWI	GGATC	3 cut(s)	12, 1627, 1640
AcoI	YGGCCR	3 cut(s)	393, 1034, 1281
AcsI	RAATTY	4 cut(s)	779, 1182, 1835, 2251
AcyI	GRCGYC	1 cut(s)	887
AfaI	GTAC	5 cut(s)	368, 776, 1517, 2177, 2234
AfiI	CCNNNNNNNGG	5 cut(s)	82, 378, 1360, 1551, 1577
AflIII	ACRYGT	3 cut(s)	771, 1158, 1723
AgsI	TTSAA	5 cut(s)	421, 590, 635, 787, 2199
AjiI	CACGTC	1 cut(s)	1161
AjnI	CCWGG	3 cut(s)	81, 314, 1680
AjuI	GAANNNNNNNTTGG	6 cut(s)	389, 413, 421, 445, 790, 822
AleI	CACNNNNGTG	1 cut(s)	911
AluBI	AGCT	8 cut(s)	324, 343, 358, 556, 854, 953, 1621, 2119
AluI	AGCT	8 cut(s)	324, 343, 358, 556, 854, 953, 1621, 2119
Alw21I	GWGCWC	3 cut(s)	326, 513, 558
Alw26I	GTCTC	5 cut(s)	1242, 1326, 1546, 1630, 1879
AlwI	GGATC	3 cut(s)	12, 1627, 1640
AlwNI	CAGNNNCTG	3 cut(s)	82, 1019, 2128
Ama87I	CYCGRG	2 cut(s)	1193, 1733
AoxI	GGCC	6 cut(s)	393, 1034, 1281, 1603, 2167, 2261
ApeKI	GCWGC	6 cut(s)	24, 460, 574, 953, 1061, 2119
ApoI	RAATTY	4 cut(s)	779, 1182, 1835, 2251
ArsI	GACNNNNNNTTYG	2 cut(s)	1820, 1852
Asp700I	GAANNNNTTC	2 cut(s)	150, 1014
AspS9I	GGNCC	5 cut(s)	645, 1447, 1568, 1763, 2261
AsuC2I	CCSGG	5 cut(s)	307, 596, 1451, 1595, 1637
AsuHPI	GGTGA	5 cut(s)	28, 146, 331, 512, 898
AsuII	TTCGAA	2 cut(s)	1166, 1839
AvaI	CYCGRG	2 cut(s)	1193, 1733
AvaII	GGWCC	4 cut(s)	645, 1447, 1568, 1763
BamHI	GGATCC	1 cut(s)	1632
BanI	GGYRCC	1 cut(s)	1502
BanII	GRGCYC	2 cut(s)	326, 558
BbsI	GAAGAC	4 cut(s)	59, 797, 1155, 1926
Bbv12I	GWGCWC	3 cut(s)	326, 513, 558
BbvI	GCAGC	6 cut(s)	36, 447, 586, 940, 1048, 2106
BccI	CCATC	9 cut(s)	36, 371, 497, 1026, 1514, 1553, 1961, 2125, 2305
BceAI	ACGGC	3 cut(s)	408, 1268, 1516
BcgI	CGANNNNNNTGC	2 cut(s)	2001, 2035
BciT130I	CCWGG	3 cut(s)	83, 316, 1682
BclI	TGATCA	2 cut(s)	681, 2220
BcnI	CCSGG	5 cut(s)	307, 596, 1451, 1595, 1637
BcoDI	GTCTC	5 cut(s)	1242, 1326, 1546, 1630, 1879
BfaI	CTAG	6 cut(s)	935, 966, 1325, 1577, 1850, 2300
BfmI	CTRYAG	3 cut(s)	1983, 2043, 2120
BfuAI	ACCTGC	1 cut(s)	1319
BglII	AGATCT	1 cut(s)	1846
BisI	GCNGC	7 cut(s)	22, 25, 461, 575, 954, 1062, 2120
BlsI	GCNGC	7 cut(s)	23, 26, 462, 576, 955, 1063, 2121
Bme1390I	CCNGG	8 cut(s)	83, 307, 316, 596, 1451, 1595, 1637, 1682
Bme18I	GGWCC	4 cut(s)	645, 1447, 1568, 1763
BmeT110I	CYCGRG	2 cut(s)	1193, 1733
BmgBI	CACGTC	1 cut(s)	1161
BmgT120I	GGNCC	5 cut(s)	645, 1447, 1568, 1763, 2261
BmiI	GGNNCC	8 cut(s)	646, 647, 1390, 1448, 1449, 1504, 1556, 1634
BmrFI	CCNGG	8 cut(s)	83, 307, 316, 596, 1451, 1595, 1637, 1682
BmrI	ACTGGG	1 cut(s)	1709
BmsI	GCATC	7 cut(s)	586, 708, 979, 1280, 1424, 1710, 1778
BmuI	ACTGGG	1 cut(s)	1709
BpiI	GAAGAC	4 cut(s)	59, 797, 1155, 1926
BplI	GAGNNNNNCTC	4 cut(s)	1610, 1642, 2269, 2301
BpmI	CTGGAG	2 cut(s)	29, 572
Bpu14I	TTCGAA	2 cut(s)	1166, 1839
BpuEI	CTTGAG	2 cut(s)	1415, 2263
BpuMI	CCSGG	5 cut(s)	307, 596, 1451, 1595, 1637
Bsa29I	ATCGAT	2 cut(s)	580, 2097
BsaAI	YACGTR	2 cut(s)	2179, 2323
BsaHI	GRCGYC	1 cut(s)	887
BsaI	GGTCTC	1 cut(s)	1879
BsaJI	CCNNGG	8 cut(s)	82, 315, 816, 1139, 1260, 1594, 1734, 2019
Bsc4I	CCNNNNNNNGG	5 cut(s)	82, 378, 1360, 1551, 1577
Bse118I	RCCGGY	2 cut(s)	1036, 1776
Bse1I	ACTGG	5 cut(s)	1096, 1208, 1556, 1715, 1920
BseBI	CCWGG	3 cut(s)	83, 316, 1682
BseCI	ATCGAT	2 cut(s)	580, 2097
BseDI	CCNNGG	8 cut(s)	82, 315, 816, 1139, 1260, 1594, 1734, 2019
BseGI	GGATG	5 cut(s)	28, 816, 1188, 1831, 2316
BseLI	CCNNNNNNNGG	5 cut(s)	82, 378, 1360, 1551, 1577
BseMII	CTCAG	6 cut(s)	174, 1011, 1425, 1573, 1608, 1915
BseNI	ACTGG	5 cut(s)	1096, 1208, 1556, 1715, 1920
BseRI	GAGGAG	6 cut(s)	105, 188, 368, 1547, 2129, 2260
BseXI	GCAGC	6 cut(s)	36, 447, 586, 940, 1048, 2106
BshFI	GGCC	6 cut(s)	395, 1036, 1283, 1605, 2169, 2263
BshNI	GGYRCC	1 cut(s)	1502
BshVI	ATCGAT	2 cut(s)	580, 2097
BsiHKAI	GWGCWC	3 cut(s)	326, 513, 558
BsiHKCI	CYCGRG	2 cut(s)	1193, 1733
BsiSI	CCGG	7 cut(s)	307, 596, 1037, 1451, 1594, 1637, 1777
BsiWI	CGTACG	1 cut(s)	2175
BslFI	GGGAC	7 cut(s)	292, 578, 658, 960, 1433, 1697, 1703
BslI	CCNNNNNNNGG	5 cut(s)	82, 378, 1360, 1551, 1577
BsmAI	GTCTC	5 cut(s)	1242, 1326, 1546, 1630, 1879
BsmFI	GGGAC	7 cut(s)	292, 578, 658, 960, 1433, 1697, 1703
BsmI	GAATGC	2 cut(s)	1898, 2280
BsnI	GGCC	6 cut(s)	395, 1036, 1283, 1605, 2169, 2263
Bso31I	GGTCTC	1 cut(s)	1879
BsoBI	CYCGRG	2 cut(s)	1193, 1733
Bsp119I	TTCGAA	2 cut(s)	1166, 1839
Bsp1286I	GDGCHC	3 cut(s)	326, 513, 558
Bsp1407I	TGTACA	1 cut(s)	774
Bsp143I	GATC	6 cut(s)	4, 681, 865, 1632, 1846, 2220
Bsp19I	CCATGG	1 cut(s)	1260
BspACI	CCGC	4 cut(s)	22, 537, 822, 1064
BspANI	GGCC	6 cut(s)	395, 1036, 1283, 1605, 2169, 2263
BspCNI	CTCAG	6 cut(s)	173, 1012, 1424, 1572, 1609, 1914
BspDI	ATCGAT	2 cut(s)	580, 2097
BspLI	GGNNCC	8 cut(s)	646, 647, 1390, 1448, 1449, 1504, 1556, 1634
BspMAI	CTGCAG	1 cut(s)	2124
BspMI	ACCTGC	1 cut(s)	1319
BspPI	GGATC	3 cut(s)	12, 1627, 1640
BspT104I	TTCGAA	2 cut(s)	1166, 1839
BspT107I	GGYRCC	1 cut(s)	1502
BspTNI	GGTCTC	1 cut(s)	1879
BsrFI	RCCGGY	2 cut(s)	1036, 1776
BsrGI	TGTACA	1 cut(s)	774
BsrI	ACTGG	5 cut(s)	1096, 1208, 1556, 1715, 1920
BssAI	RCCGGY	2 cut(s)	1036, 1776
BssECI	CCNNGG	8 cut(s)	82, 315, 816, 1139, 1260, 1594, 1734, 2019
BssMI	GATC	6 cut(s)	4, 681, 865, 1632, 1846, 2220
BssNAI	GTATAC	2 cut(s)	718, 827
BssNI	GRCGYC	1 cut(s)	887
BssT1I	CCWWGG	3 cut(s)	816, 1139, 1260
Bst1107I	GTATAC	2 cut(s)	718, 827
Bst2UI	CCWGG	3 cut(s)	83, 316, 1682
Bst4CI	ACNGT	4 cut(s)	401, 1294, 1811, 1939
Bst6I	CTCTTC	4 cut(s)	1361, 1750, 2054, 2183
BstACI	GRCGYC	1 cut(s)	887
BstAPI	GCANNNNNTGC	1 cut(s)	445
BstAUI	TGTACA	1 cut(s)	774
BstBAI	YACGTR	2 cut(s)	2179, 2323
BstBI	TTCGAA	2 cut(s)	1166, 1839
BstC8I	GCNNGC	6 cut(s)	312, 341, 345, 1343, 1798, 2014
BstDEI	CTNAG	9 cut(s)	160, 251, 1020, 1411, 1559, 1617, 1747, 1901, 1963
BstDSI	CCRYGG	1 cut(s)	1260
BstEII	GGTNACC	1 cut(s)	1805
BstENI	CCTNNNNNAGG	1 cut(s)	1575
BstF5I	GGATG	5 cut(s)	28, 816, 1188, 1831, 2316
BstKTI	GATC	6 cut(s)	7, 684, 868, 1635, 1849, 2223
BstMAI	GTCTC	5 cut(s)	1242, 1326, 1546, 1630, 1879
BstMBI	GATC	6 cut(s)	4, 681, 865, 1632, 1846, 2220
BstMWI	GCNNNNNNNGC	8 cut(s)	307, 401, 445, 860, 1058, 1797, 2144, 2153
BstNI	CCWGG	3 cut(s)	83, 316, 1682
BstNSI	RCATGY	2 cut(s)	775, 1727
BstPI	GGTNACC	1 cut(s)	1805
BstSCI	CCNGG	8 cut(s)	81, 305, 314, 594, 1449, 1593, 1635, 1680
BstSFI	CTRYAG	3 cut(s)	1983, 2043, 2120
BstV1I	GCAGC	6 cut(s)	36, 447, 586, 940, 1048, 2106
BstV2I	GAAGAC	4 cut(s)	59, 797, 1155, 1926
BstX2I	RGATCY	3 cut(s)	4, 1632, 1846
BstYI	RGATCY	3 cut(s)	4, 1632, 1846
BstZ17I	GTATAC	2 cut(s)	718, 827
Bsu15I	ATCGAT	2 cut(s)	580, 2097
BsuRI	GGCC	6 cut(s)	395, 1036, 1283, 1605, 2169, 2263
BsuTUI	ATCGAT	2 cut(s)	580, 2097
BtgI	CCRYGG	1 cut(s)	1260
BtgZI	GCGATG	1 cut(s)	1401
BtrI	CACGTC	1 cut(s)	1161
BtsCI	GGATG	5 cut(s)	28, 816, 1188, 1831, 2316
BtsIMutI	CAGTG	3 cut(s)	406, 926, 1614
BveI	ACCTGC	1 cut(s)	1319
Cac8I	GCNNGC	6 cut(s)	312, 341, 345, 1343, 1798, 2014
CaiI	CAGNNNCTG	3 cut(s)	82, 1019, 2128
Cfr10I	RCCGGY	2 cut(s)	1036, 1776
Cfr13I	GGNCC	5 cut(s)	645, 1447, 1568, 1763, 2261
ClaI	ATCGAT	2 cut(s)	580, 2097
CseI	GACGC	2 cut(s)	64, 223
CsiI	ACCWGGT	1 cut(s)	1680
Csp6I	GTAC	5 cut(s)	367, 775, 1516, 2176, 2233
CviQI	GTAC	5 cut(s)	367, 775, 1516, 2176, 2233
DdeI	CTNAG	9 cut(s)	160, 251, 1020, 1411, 1559, 1617, 1747, 1901, 1963
DpnI	GATC	6 cut(s)	6, 683, 867, 1634, 1848, 2222
DpnII	GATC	6 cut(s)	4, 681, 865, 1632, 1846, 2220
EaeI	YGGCCR	3 cut(s)	393, 1034, 1281
Eam1104I	CTCTTC	4 cut(s)	1361, 1750, 2054, 2183
EarI	CTCTTC	4 cut(s)	1361, 1750, 2054, 2183
Ecl136II	GAGCTC	2 cut(s)	324, 556
Eco130I	CCWWGG	3 cut(s)	816, 1139, 1260
Eco24I	GRGCYC	2 cut(s)	326, 558
Eco31I	GGTCTC	1 cut(s)	1879
Eco47I	GGWCC	4 cut(s)	645, 1447, 1568, 1763
Eco53kI	GAGCTC	2 cut(s)	324, 556
Eco88I	CYCGRG	2 cut(s)	1193, 1733
Eco91I	GGTNACC	1 cut(s)	1805
EcoICRI	GAGCTC	2 cut(s)	324, 556
EcoNI	CCTNNNNNAGG	1 cut(s)	1575
EcoO109I	RGGNCCY	3 cut(s)	645, 1447, 1568
EcoO65I	GGTNACC	1 cut(s)	1805
EcoRI	GAATTC	1 cut(s)	1835
EcoRII	CCWGG	3 cut(s)	81, 314, 1680
EcoT14I	CCWWGG	3 cut(s)	816, 1139, 1260
EcoT22I	ATGCAT	2 cut(s)	1273, 1793
EcoT38I	GRGCYC	2 cut(s)	326, 558
ErhI	CCWWGG	3 cut(s)	816, 1139, 1260
FaqI	GGGAC	7 cut(s)	292, 578, 658, 960, 1433, 1697, 1703
FbaI	TGATCA	2 cut(s)	681, 2220
FblI	GTMKAC	4 cut(s)	717, 826, 1986, 2316
Fnu4HI	GCNGC	7 cut(s)	22, 25, 461, 575, 954, 1062, 2120
FokI	GGATG	5 cut(s)	15, 823, 1175, 1838, 2323
FriOI	GRGCYC	2 cut(s)	326, 558
Fsp4HI	GCNGC	7 cut(s)	22, 25, 461, 575, 954, 1062, 2120
FspBI	CTAG	6 cut(s)	935, 966, 1325, 1577, 1850, 2300
GluI	GCNGC	7 cut(s)	22, 25, 461, 575, 954, 1062, 2120
GsuI	CTGGAG	2 cut(s)	29, 572
HaeIII	GGCC	6 cut(s)	395, 1036, 1283, 1605, 2169, 2263
HapII	CCGG	7 cut(s)	307, 596, 1037, 1451, 1594, 1637, 1777
HgaI	GACGC	2 cut(s)	64, 223
Hin1I	GRCGYC	1 cut(s)	887
HincII	GTYRAC	1 cut(s)	63
HindII	GTYRAC	1 cut(s)	63
HinfI	GANTC	8 cut(s)	44, 220, 736, 931, 1010, 1590, 1743, 2094
HpaI	GTTAAC	1 cut(s)	63
HpaII	CCGG	7 cut(s)	307, 596, 1037, 1451, 1594, 1637, 1777
HphI	GGTGA	5 cut(s)	28, 146, 331, 512, 898
Hpy166II	GTNNAC	9 cut(s)	63, 127, 369, 718, 827, 919, 1819, 1987, 2317
Hpy8I	GTNNAC	9 cut(s)	63, 127, 369, 718, 827, 919, 1819, 1987, 2317
Hpy99I	CGWCG	2 cut(s)	263, 394
HpyAV	CCTTC	5 cut(s)	34, 266, 641, 1430, 2176
HpyCH4III	ACNGT	4 cut(s)	401, 1294, 1811, 1939
HpyCH4IV	ACGT	4 cut(s)	887, 1160, 2178, 2322
HpyF10VI	GCNNNNNNNGC	8 cut(s)	307, 401, 445, 860, 1058, 1797, 2144, 2153
HpyF3I	CTNAG	9 cut(s)	160, 251, 1020, 1411, 1559, 1617, 1747, 1901, 1963
HpySE526I	ACGT	4 cut(s)	887, 1160, 2178, 2322
Hsp92I	GRCGYC	1 cut(s)	887
KflI	GGGWCCC	2 cut(s)	645, 1447
Ksp22I	TGATCA	2 cut(s)	681, 2220
KspAI	GTTAAC	1 cut(s)	63
Kzo9I	GATC	6 cut(s)	4, 681, 865, 1632, 1846, 2220
LmnI	GCTCC	6 cut(s)	329, 355, 553, 1394, 1560, 2116
Lsp1109I	GCAGC	6 cut(s)	36, 447, 586, 940, 1048, 2106
LweI	GCATC	7 cut(s)	586, 708, 979, 1280, 1424, 1710, 1778
MabI	ACCWGGT	1 cut(s)	1680
MaeI	CTAG	6 cut(s)	935, 966, 1325, 1577, 1850, 2300
MaeII	ACGT	4 cut(s)	887, 1160, 2178, 2322
MaeIII	GTNAC	5 cut(s)	924, 1231, 1303, 1657, 1805
MalI	GATC	6 cut(s)	6, 683, 867, 1634, 1848, 2222
MboI	GATC	6 cut(s)	4, 681, 865, 1632, 1846, 2220
MfeI	CAATTG	2 cut(s)	334, 675
MflI	RGATCY	3 cut(s)	4, 1632, 1846
MhlI	GDGCHC	3 cut(s)	326, 513, 558
MlyI	GAGTC	1 cut(s)	1599
MmeI	TCCRAC	3 cut(s)	413, 788, 1924
Mph1103I	ATGCAT	2 cut(s)	1273, 1793
MroXI	GAANNNNTTC	2 cut(s)	150, 1014
MseI	TTAA	4 cut(s)	62, 104, 1200, 1475
MslI	CAYNNNNRTG	4 cut(s)	582, 911, 1824, 1976
MspA1I	CMGCKG	2 cut(s)	343, 539
MspI	CCGG	7 cut(s)	307, 596, 1037, 1451, 1594, 1637, 1777
MspR9I	CCNGG	8 cut(s)	83, 307, 316, 596, 1451, 1595, 1637, 1682
MunI	CAATTG	2 cut(s)	334, 675
Mva1269I	GAATGC	2 cut(s)	1898, 2280
MvaI	CCWGG	3 cut(s)	83, 316, 1682
MwoI	GCNNNNNNNGC	8 cut(s)	307, 401, 445, 860, 1058, 1797, 2144, 2153
NciI	CCSGG	5 cut(s)	307, 596, 1451, 1595, 1637
NcoI	CCATGG	1 cut(s)	1260
NdeII	GATC	6 cut(s)	4, 681, 865, 1632, 1846, 2220
NlaIV	GGNNCC	8 cut(s)	646, 647, 1390, 1448, 1449, 1504, 1556, 1634
NmeAIII	GCCGAG	1 cut(s)	2289
NmuCI	GTSAC	1 cut(s)	924
NsiI	ATGCAT	2 cut(s)	1273, 1793
NspI	RCATGY	2 cut(s)	775, 1727
NspV	TTCGAA	2 cut(s)	1166, 1839
OliI	CACNNNNGTG	1 cut(s)	911
PciI	ACATGT	2 cut(s)	771, 1723
PcsI	WCGNNNNNNNCGW	1 cut(s)	267
PctI	GAATGC	2 cut(s)	1898, 2280
PdmI	GAANNNNTTC	2 cut(s)	150, 1014
PfeI	GAWTC	7 cut(s)	44, 220, 736, 931, 1010, 1743, 2094
Pfl23II	CGTACG	1 cut(s)	2175
PflMI	CCANNNNNTGG	2 cut(s)	82, 1551
PkrI	GCNGC	7 cut(s)	23, 26, 462, 576, 955, 1063, 2121
PleI	GAGTC	1 cut(s)	1598
PpsI	GAGTC	1 cut(s)	1598
Ppu21I	YACGTR	2 cut(s)	2179, 2323
PpuMI	RGGWCCY	3 cut(s)	645, 1447, 1568
PscI	ACATGT	2 cut(s)	771, 1723
PsiI	TTATAA	1 cut(s)	1088
Psp124BI	GAGCTC	2 cut(s)	326, 558
Psp5II	RGGWCCY	3 cut(s)	645, 1447, 1568
Psp6I	CCWGG	3 cut(s)	81, 314, 1680
PspEI	GGTNACC	1 cut(s)	1805
PspGI	CCWGG	3 cut(s)	81, 314, 1680
PspLI	CGTACG	1 cut(s)	2175
PspN4I	GGNNCC	8 cut(s)	646, 647, 1390, 1448, 1449, 1504, 1556, 1634
PspPI	GGNCC	5 cut(s)	645, 1447, 1568, 1763, 2261
PspPPI	RGGWCCY	3 cut(s)	645, 1447, 1568
PstI	CTGCAG	1 cut(s)	2124
PstNI	CAGNNNCTG	3 cut(s)	82, 1019, 2128
PsuI	RGATCY	3 cut(s)	4, 1632, 1846
PvuII	CAGCTG	1 cut(s)	343
RsaI	GTAC	5 cut(s)	368, 776, 1517, 2177, 2234
RsaNI	GTAC	5 cut(s)	367, 775, 1516, 2176, 2233
RseI	CAYNNNNRTG	4 cut(s)	582, 911, 1824, 1976
SacI	GAGCTC	2 cut(s)	326, 558
SaqAI	TTAA	4 cut(s)	62, 104, 1200, 1475
SatI	GCNGC	7 cut(s)	22, 25, 461, 575, 954, 1062, 2120
Sau3AI	GATC	6 cut(s)	4, 681, 865, 1632, 1846, 2220
Sau96I	GGNCC	5 cut(s)	645, 1447, 1568, 1763, 2261
SchI	GAGTC	1 cut(s)	1599
ScrFI	CCNGG	8 cut(s)	83, 307, 316, 596, 1451, 1595, 1637, 1682
SduI	GDGCHC	3 cut(s)	326, 513, 558
SexAI	ACCWGGT	1 cut(s)	1680
SfaNI	GCATC	7 cut(s)	586, 708, 979, 1280, 1424, 1710, 1778
SfcI	CTRYAG	3 cut(s)	1983, 2043, 2120
SfuI	TTCGAA	2 cut(s)	1166, 1839
SinI	GGWCC	4 cut(s)	645, 1447, 1568, 1763
SmiMI	CAYNNNNRTG	4 cut(s)	582, 911, 1824, 1976
SmlI	CTYRAG	2 cut(s)	1394, 2242
SmoI	CTYRAG	2 cut(s)	1394, 2242
SsiI	CCGC	4 cut(s)	22, 537, 822, 1064
SspMI	CTAG	6 cut(s)	935, 966, 1325, 1577, 1850, 2300
SstI	GAGCTC	2 cut(s)	326, 558
StyD4I	CCNGG	8 cut(s)	81, 305, 314, 594, 1449, 1593, 1635, 1680
StyI	CCWWGG	3 cut(s)	816, 1139, 1260
TaaI	ACNGT	4 cut(s)	401, 1294, 1811, 1939
TaiI	ACGT	4 cut(s)	890, 1163, 2181, 2325
TaqII	GACCGA	1 cut(s)	1866
TatI	WGTACW	2 cut(s)	366, 774
TauI	GCSGC	1 cut(s)	24
TfiI	GAWTC	7 cut(s)	44, 220, 736, 931, 1010, 1743, 2094
Tru1I	TTAA	4 cut(s)	62, 104, 1200, 1475
Tru9I	TTAA	4 cut(s)	62, 104, 1200, 1475
TscAI	CASTG	3 cut(s)	406, 933, 1621
TseFI	GTSAC	1 cut(s)	924
TseI	GCWGC	6 cut(s)	24, 460, 574, 953, 1061, 2119
Tsp45I	GTSAC	1 cut(s)	924
TspDTI	ATGAA	7 cut(s)	36, 1023, 1452, 1456, 1861, 1941, 2222
TspGWI	ACGGA	1 cut(s)	1699
TspRI	CASTG	3 cut(s)	406, 933, 1621
Van91I	CCANNNNNTGG	2 cut(s)	82, 1551
VpaK11BI	GGWCC	4 cut(s)	645, 1447, 1568, 1763
XagI	CCTNNNNNAGG	1 cut(s)	1575
XapI	RAATTY	4 cut(s)	779, 1182, 1835, 2251
XbaI	TCTAGA	1 cut(s)	2299
XceI	RCATGY	2 cut(s)	775, 1727
XcmI	CCANNNNNNNNNTGG	1 cut(s)	340
XmiI	GTMKAC	4 cut(s)	717, 826, 1986, 2316
XmnI	GAANNNNTTC	2 cut(s)	150, 1014
XspI	CTAG	6 cut(s)	935, 966, 1325, 1577, 1850, 2300
ZraI	GACGTC	1 cut(s)	888
Zsp2I	ATGCAT	2 cut(s)	1273, 1793

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.