Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0011810

Physical Location & Seq

Genomic Coordinates Forward (+)

2728427 .. 2733624

Gene Structure 5198 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1037500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2328 bp

ATGGATCTGGAGGGTGTGATCCGCAGCCATCCAATCAAGAAAGATTCATGGATGACGGTGTTAACTCTGGCTTACCAGAGCCTGGGAGTAGTCTATGGAGATTTAAGCACGTCTCCTCTCTATGTCTACAAAAGCACTTTTGCAGAAGACATTCACCACTCAGAAACCAATGAGGAGATTTTTGGGGTTCTCTCGTTTGTGTTCTGGACGCTGACGCTGATTCCATTAGTGAAATATGTGTTTATTGTGCTTAGAGCCGACGACAATGGCGAAGGTGGGACTTTTGCTCTGTATTCTCTGCTTTCCCGGCACGCCCGGGTGAGCTCCTTGCCCAATTGCCAGCTGGCGGATGAGGAGCTGTCGGAGTACACAAAAGATGGGGTTGTTCCGACAGCGAACAGCGCTTTCGGGTCGAGTTTGAAATCGACATTGGAGAAGCATAAGGCGCTGCAAAAGGTGCTGCTTGTTCTGGCTTTGATTGGAACTTGTATGGTTATTGGTGATGGGGTGCTCACACCAGCAATTTCTGTGTTTTCCGCTGTTTCGGGATTGGAGCTCTCCATGTCGAAAGAGCAGCATCGTTATGTTGAAGTCCCTGTTGCTTGTGTCATCCTCATATTTCTGTTTGCCCTTCAACATTACGGGACTCACCGAGTAGGGTTCTTGTTTGCACCAGTTGTGATCACATGGCTTTTGTGCATCAGTTCCATTGGTGTATACAACATCATTTACTGGAATCCCCATGTTTATCAGGCACTCTCTCCATACTACATGTACAAATTTTTTAAGAAGACTCGAAAAGGAGGTTGGATGTCCTTGGGCGGTATACTGTTGTGCATGACAGGCTCGGAAGCTATGTTTGCTGATCTTGGACACTTTTCACAGACGTCAATCAAGATTGCTTTCACGTTTGTGGTTTACCCGTCCTTGATTCTAGCATACATGGGACAAGCTGCATACCTTTCTAAGCATCATATCATAGAAAGTGATTATCGAATTGGATTCTATGAATCAGTTCCCGAGAAATTAAGATGGCCCGTCTTGGCAATAGCCATACTTGCTGCAGTGGTGGGAAGTCAAGCAATCATAACTGGAACTTTCTCGATAATCAAACAATGTTCTGCTTTGGGTTGCTTTCCAAGGGTCAAGATTGTCCACACATCTTCGAAAATACACGGTCAAATTTACATCCCTGAGATTAACTGGACTTTGATGTTTCTATGCTTGGCTGTTACCATTGGTTTTAGAGACACAAAAGCCATGGGAAATGCATCAGGTTTGGCCGTTATAACTGTCATGCTGGTAACTACCTGCCTTATGTCTCTAGTCATAGTCTTGTGCTGGCACAAAAGCCTCTTCTGGGCAATTTGCTTTATATTATTCTTCGGCTCCCTTGAGGCACTCTACTTCTCAGCATCTCTCATGAAGTTTCGTGAAGGGGCTTGGGTCCCGATTGCTCTTTCATTCATCTTCTTAATGGTTATGTATGTTTGGCACTACGGCACCTTCAAGAAATACGAGTTTGACGTCCAAAATAAAGTCTCCATCAACTGGCTCCTCAGTTTGGGTCCTACTCTAGGGATTGTTCGAGTCCGGGGGATTGGCCTTATACACACTGAGCTCGTCTCTGGGATCCCGGCTATTTTCTCTCACTTTGTTACCAACCTCCCTGCTTTCCATCAGGTTGTAGTTTTCCTCTGCATCAAATCCGTCCCAGTCCCACATGTCAGACCCGAGGAAAGATTCTTAGTGGGAAGAGTTGGTCCAAAGGAATACCGGCTATATAGATGCATAGCACGCTATGGTTACCGTGATGTTCACAAGGATGACATGGAATTCGAAAGAGATCTGATTTGTAGTATTGCAGAGTTCATTCGGTCAGAGAGACCAGAATGCAATCTGAGTCTAGTAAAATTGGAAGATGATGAGAAAATGACAGTTGTTGGGACTTCATCATCAAATTTAGACGGCATAAGAATGTCTGTAGCTGAACCGGATTCCTCCGAAATAGCAAGCACCTCAGAGTTGCAGGAGATAGGGCCTACCGAAAAGGCAAAGAAGAGAGTGAAGTTCGTTGTTCCAGAAACCCCACGAATCGACAGGGAAGCAGTAGGGGAGCTGCAGGAACTGATGGAAGCAAGGGAGGCAGGAATGGCATTTATATTAGGCCACTCTTACGTGAAGGCGAAGAGAGGATCAAGTTTTATGAAGAAAATAGTGATCAATTTCGGGTACGATTTCTTAAGGAGGAATTTTCGAGGGCCGAGTTATGCTTTGAGCATTCCTCATGTATCTACTCTGGAGGTTGGGATGGTCTACCACGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

776

Amino Acids

86.89

Weight (kDa)

8.33

Isoelectric Point (pI)

39.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	23 - 520	7.6e-172	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	533 - 774	1.5e-26	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011810)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14880
fragaria_vesca	FvH4_6g50500 FvH4_6g50500
malus_domestica	MD09G1035900.v1.1 MD17G1037500.v1.1
prunus_persica	Prupe.3G281900_v2.0.a1 Prupe.3G281900_v2.0.a1
pyrus_communis	pycom111g02870 pycom17g03360
rosa_chinensis	RchiOBHm_Chr2g0170921
rosa_laevigata	RLG00000021992
rosa_multiflora	Rmu_sc0009938.1_g000001
rosa_roxburghii	Rroxscaffold_2G00080980
rosa_rugosa	Rorug02G0552300
rosa_samantha	Rh2AG625000 Rh2BG635700 Rh2CG604400 Rh2DG647300
rosa_wichuraiana	Rw2G051770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1289
AatII	GACGTC	2 cut(s)	890, 1530
Acc36I	ACCTGC	1 cut(s)	1319
AccB1I	GGYRCC	1 cut(s)	1502
AccB7I	CCANNNNNTGG	2 cut(s)	82, 1551
AccI	GTMKAC	4 cut(s)	126, 717, 826, 2316
AciI	CCGC	4 cut(s)	22, 347, 537, 822
AclWI	GGATC	5 cut(s)	12, 13, 1627, 1640, 2203
AcoI	YGGCCR	1 cut(s)	1281
AcsI	RAATTY	5 cut(s)	779, 1182, 1835, 1960, 2251
AcyI	GRCGYC	2 cut(s)	887, 1527
AfaI	GTAC	3 cut(s)	368, 776, 2234
AfeI	AGCGCT	1 cut(s)	403
AfiI	CCNNNNNNNGG	6 cut(s)	82, 346, 1360, 1551, 1564, 1577
AflII	CTTAAG	1 cut(s)	2242
AflIII	ACRYGT	2 cut(s)	771, 1723
AgsI	TTSAA	4 cut(s)	421, 590, 635, 1510
AjiI	CACGTC	1 cut(s)	111
AjnI	CCWGG	1 cut(s)	81
AjuI	GAANNNNNNNTTGG	4 cut(s)	413, 445, 790, 822
AleI	CACNNNNGTG	1 cut(s)	911
AluBI	AGCT	9 cut(s)	324, 343, 358, 556, 854, 953, 1621, 1988, 2119
AluI	AGCT	9 cut(s)	324, 343, 358, 556, 854, 953, 1621, 1988, 2119
Alw21I	GWGCWC	4 cut(s)	326, 513, 558, 1623
Alw26I	GTCTC	6 cut(s)	117, 1242, 1326, 1546, 1630, 1879
AlwI	GGATC	5 cut(s)	12, 13, 1627, 1640, 2203
AlwNI	CAGNNNCTG	2 cut(s)	82, 2128
Ama87I	CYCGRG	3 cut(s)	315, 1019, 1733
Aor51HI	AGCGCT	1 cut(s)	403
AoxI	GGCC	6 cut(s)	1034, 1281, 1603, 2039, 2167, 2261
ApeKI	GCWGC	7 cut(s)	24, 448, 460, 574, 953, 1061, 2119
ApoI	RAATTY	5 cut(s)	779, 1182, 1835, 1960, 2251
ArsI	GACNNNNNNTTYG	2 cut(s)	1820, 1852
Asp700I	GAANNNNTTC	2 cut(s)	150, 1014
AspLEI	GCGC	2 cut(s)	404, 448
AspS9I	GGNCC	6 cut(s)	1035, 1447, 1568, 1763, 2039, 2261
AsuC2I	CCSGG	5 cut(s)	307, 316, 317, 1595, 1637
AsuHPI	GGTGA	4 cut(s)	146, 331, 512, 641
AsuII	TTCGAA	2 cut(s)	1166, 1839
AvaI	CYCGRG	3 cut(s)	315, 1019, 1733
AvaII	GGWCC	3 cut(s)	1447, 1568, 1763
BamHI	GGATCC	1 cut(s)	1632
BanI	GGYRCC	1 cut(s)	1502
BanII	GRGCYC	3 cut(s)	326, 558, 1623
BbsI	GAAGAC	2 cut(s)	153, 797
Bbv12I	GWGCWC	4 cut(s)	326, 513, 558, 1623
BbvI	GCAGC	7 cut(s)	36, 435, 447, 586, 940, 1048, 2106
BccI	CCATC	8 cut(s)	36, 371, 497, 1026, 1553, 1686, 2125, 2305
BceAI	ACGGC	3 cut(s)	1268, 1516, 1984
BciT130I	CCWGG	1 cut(s)	83
BclI	TGATCA	2 cut(s)	681, 2220
BcnI	CCSGG	5 cut(s)	307, 316, 317, 1595, 1637
BcoDI	GTCTC	6 cut(s)	117, 1242, 1326, 1546, 1630, 1879
BfaI	CTAG	4 cut(s)	935, 1325, 1577, 1907
BfmI	CTRYAG	3 cut(s)	1062, 1983, 2120
BfoI	RGCGCY	2 cut(s)	405, 449
BfrI	CTTAAG	1 cut(s)	2242
BfuAI	ACCTGC	1 cut(s)	1319
BglII	AGATCT	1 cut(s)	1846
BisI	GCNGC	7 cut(s)	25, 449, 461, 575, 954, 1062, 2120
BlsI	GCNGC	7 cut(s)	26, 450, 462, 576, 955, 1063, 2121
Bme1390I	CCNGG	6 cut(s)	83, 307, 316, 317, 1595, 1637
Bme18I	GGWCC	3 cut(s)	1447, 1568, 1763
BmeT110I	CYCGRG	3 cut(s)	315, 1019, 1733
BmgBI	CACGTC	1 cut(s)	111
BmgT120I	GGNCC	6 cut(s)	1035, 1447, 1568, 1763, 2039, 2261
BmiI	GGNNCC	7 cut(s)	1390, 1448, 1449, 1504, 1556, 1569, 1634
BmrFI	CCNGG	6 cut(s)	83, 307, 316, 317, 1595, 1637
BmrI	ACTGGG	1 cut(s)	1709
BmsI	GCATC	7 cut(s)	586, 708, 979, 1280, 1424, 1710, 1778
BmuI	ACTGGG	1 cut(s)	1709
BpiI	GAAGAC	2 cut(s)	153, 797
BplI	GAGNNNNNCTC	4 cut(s)	1610, 1642, 2269, 2301
BpmI	CTGGAG	2 cut(s)	29, 2321
Bpu14I	TTCGAA	2 cut(s)	1166, 1839
BpuEI	CTTGAG	1 cut(s)	1415
BpuMI	CCSGG	5 cut(s)	307, 316, 317, 1595, 1637
BsaAI	YACGTR	2 cut(s)	2179, 2323
BsaHI	GRCGYC	2 cut(s)	887, 1527
BsaI	GGTCTC	1 cut(s)	1879
BsaJI	CCNNGG	7 cut(s)	82, 315, 816, 1139, 1260, 1594, 1734
BsaWI	WCCGGW	1 cut(s)	1993
Bsc4I	CCNNNNNNNGG	6 cut(s)	82, 346, 1360, 1551, 1564, 1577
Bse118I	RCCGGY	1 cut(s)	1776
Bse1I	ACTGG	6 cut(s)	674, 737, 1096, 1208, 1556, 1715
BseBI	CCWGG	1 cut(s)	83
BseDI	CCNNGG	7 cut(s)	82, 315, 816, 1139, 1260, 1594, 1734
BseGI	GGATG	8 cut(s)	28, 57, 355, 609, 816, 1188, 1831, 2316
BseLI	CCNNNNNNNGG	6 cut(s)	82, 346, 1360, 1551, 1564, 1577
BseMII	CTCAG	7 cut(s)	174, 1185, 1425, 1573, 1608, 1892, 2034
BseNI	ACTGG	6 cut(s)	674, 737, 1096, 1208, 1556, 1715
BseRI	GAGGAG	4 cut(s)	105, 188, 368, 1547
BseXI	GCAGC	7 cut(s)	36, 435, 447, 586, 940, 1048, 2106
BshFI	GGCC	6 cut(s)	1036, 1283, 1605, 2041, 2169, 2263
BshNI	GGYRCC	1 cut(s)	1502
BsiHKAI	GWGCWC	4 cut(s)	326, 513, 558, 1623
BsiHKCI	CYCGRG	3 cut(s)	315, 1019, 1733
BsiSI	CCGG	6 cut(s)	307, 316, 1594, 1637, 1777, 1994
BslFI	GGGAC	8 cut(s)	292, 578, 658, 960, 1433, 1697, 1703, 1960
BslI	CCNNNNNNNGG	6 cut(s)	82, 346, 1360, 1551, 1564, 1577
BsmAI	GTCTC	6 cut(s)	117, 1242, 1326, 1546, 1630, 1879
BsmBI	CGTCTC	2 cut(s)	117, 1630
BsmFI	GGGAC	8 cut(s)	292, 578, 658, 960, 1433, 1697, 1703, 1960
BsmI	GAATGC	2 cut(s)	1898, 2280
BsnI	GGCC	6 cut(s)	1036, 1283, 1605, 2041, 2169, 2263
Bso31I	GGTCTC	1 cut(s)	1879
BsoBI	CYCGRG	3 cut(s)	315, 1019, 1733
Bsp119I	TTCGAA	2 cut(s)	1166, 1839
Bsp1286I	GDGCHC	4 cut(s)	326, 513, 558, 1623
Bsp1407I	TGTACA	1 cut(s)	774
Bsp143I	GATC	8 cut(s)	4, 18, 681, 865, 1632, 1846, 2195, 2220
Bsp19I	CCATGG	1 cut(s)	1260
BspACI	CCGC	4 cut(s)	22, 347, 537, 822
BspANI	GGCC	6 cut(s)	1036, 1283, 1605, 2041, 2169, 2263
BspCNI	CTCAG	7 cut(s)	173, 1186, 1424, 1572, 1609, 1893, 2033
BspHI	TCATGA	1 cut(s)	1422
BspLI	GGNNCC	7 cut(s)	1390, 1448, 1449, 1504, 1556, 1569, 1634
BspMAI	CTGCAG	2 cut(s)	1066, 2124
BspMI	ACCTGC	1 cut(s)	1319
BspPI	GGATC	5 cut(s)	12, 13, 1627, 1640, 2203
BspT104I	TTCGAA	2 cut(s)	1166, 1839
BspT107I	GGYRCC	1 cut(s)	1502
BspTI	CTTAAG	1 cut(s)	2242
BspTNI	GGTCTC	1 cut(s)	1879
BsrFI	RCCGGY	1 cut(s)	1776
BsrGI	TGTACA	1 cut(s)	774
BsrI	ACTGG	6 cut(s)	674, 737, 1096, 1208, 1556, 1715
BssAI	RCCGGY	1 cut(s)	1776
BssECI	CCNNGG	7 cut(s)	82, 315, 816, 1139, 1260, 1594, 1734
BssMI	GATC	8 cut(s)	4, 18, 681, 865, 1632, 1846, 2195, 2220
BssNAI	GTATAC	2 cut(s)	718, 827
BssNI	GRCGYC	2 cut(s)	887, 1527
BssT1I	CCWWGG	3 cut(s)	816, 1139, 1260
Bst1107I	GTATAC	2 cut(s)	718, 827
Bst2UI	CCWGG	1 cut(s)	83
Bst4CI	ACNGT	6 cut(s)	58, 831, 1178, 1294, 1811, 1939
Bst6I	CTCTTC	4 cut(s)	1361, 1750, 2054, 2183
BstACI	GRCGYC	2 cut(s)	887, 1527
BstAFI	CTTAAG	1 cut(s)	2242
BstAPI	GCANNNNNTGC	1 cut(s)	457
BstAUI	TGTACA	1 cut(s)	774
BstBAI	YACGTR	2 cut(s)	2179, 2323
BstBI	TTCGAA	2 cut(s)	1166, 1839
BstC8I	GCNNGC	6 cut(s)	312, 341, 345, 1343, 1798, 2014
BstDSI	CCRYGG	1 cut(s)	1260
BstEII	GGTNACC	1 cut(s)	1805
BstENI	CCTNNNNNAGG	1 cut(s)	1575
BstF5I	GGATG	8 cut(s)	28, 57, 355, 609, 816, 1188, 1831, 2316
BstH2I	RGCGCY	2 cut(s)	405, 449
BstHHI	GCGC	2 cut(s)	404, 448
BstKTI	GATC	8 cut(s)	7, 21, 684, 868, 1635, 1849, 2198, 2223
BstMAI	GTCTC	6 cut(s)	117, 1242, 1326, 1546, 1630, 1879
BstMBI	GATC	8 cut(s)	4, 18, 681, 865, 1632, 1846, 2195, 2220
BstNI	CCWGG	1 cut(s)	83
BstNSI	RCATGY	2 cut(s)	775, 1727
BstPI	GGTNACC	1 cut(s)	1805
BstSCI	CCNGG	6 cut(s)	81, 305, 314, 315, 1593, 1635
BstSFI	CTRYAG	3 cut(s)	1062, 1983, 2120
BstV1I	GCAGC	7 cut(s)	36, 435, 447, 586, 940, 1048, 2106
BstV2I	GAAGAC	2 cut(s)	153, 797
BstX2I	RGATCY	3 cut(s)	4, 1632, 1846
BstYI	RGATCY	3 cut(s)	4, 1632, 1846
BstZ17I	GTATAC	2 cut(s)	718, 827
BsuRI	GGCC	6 cut(s)	1036, 1283, 1605, 2041, 2169, 2263
BtgI	CCRYGG	1 cut(s)	1260
BtrI	CACGTC	1 cut(s)	111
BtsCI	GGATG	8 cut(s)	28, 57, 355, 609, 816, 1188, 1831, 2316
BtsI	GCAGTG	1 cut(s)	1071
BtsIMutI	CAGTG	2 cut(s)	1071, 1614
BveI	ACCTGC	1 cut(s)	1319
Cac8I	GCNNGC	6 cut(s)	312, 341, 345, 1343, 1798, 2014
CaiI	CAGNNNCTG	2 cut(s)	82, 2128
CciI	TCATGA	1 cut(s)	1422
CfoI	GCGC	2 cut(s)	404, 448
Cfr10I	RCCGGY	1 cut(s)	1776
Cfr13I	GGNCC	6 cut(s)	1035, 1447, 1568, 1763, 2039, 2261
Cfr9I	CCCGGG	1 cut(s)	315
CseI	GACGC	2 cut(s)	217, 223
Csp6I	GTAC	3 cut(s)	367, 775, 2233
CviQI	GTAC	3 cut(s)	367, 775, 2233
DpnI	GATC	8 cut(s)	6, 20, 683, 867, 1634, 1848, 2197, 2222
DpnII	GATC	8 cut(s)	4, 18, 681, 865, 1632, 1846, 2195, 2220
EaeI	YGGCCR	1 cut(s)	1281
Eam1104I	CTCTTC	4 cut(s)	1361, 1750, 2054, 2183
EarI	CTCTTC	4 cut(s)	1361, 1750, 2054, 2183
EciI	GGCGGA	1 cut(s)	362
Ecl136II	GAGCTC	3 cut(s)	324, 556, 1621
Eco130I	CCWWGG	3 cut(s)	816, 1139, 1260
Eco24I	GRGCYC	3 cut(s)	326, 558, 1623
Eco31I	GGTCTC	1 cut(s)	1879
Eco47I	GGWCC	3 cut(s)	1447, 1568, 1763
Eco47III	AGCGCT	1 cut(s)	403
Eco53kI	GAGCTC	3 cut(s)	324, 556, 1621
Eco88I	CYCGRG	3 cut(s)	315, 1019, 1733
Eco91I	GGTNACC	1 cut(s)	1805
EcoICRI	GAGCTC	3 cut(s)	324, 556, 1621
EcoNI	CCTNNNNNAGG	1 cut(s)	1575
EcoO109I	RGGNCCY	3 cut(s)	1447, 1568, 2039
EcoO65I	GGTNACC	1 cut(s)	1805
EcoRI	GAATTC	1 cut(s)	1835
EcoRII	CCWGG	1 cut(s)	81
EcoT14I	CCWWGG	3 cut(s)	816, 1139, 1260
EcoT22I	ATGCAT	2 cut(s)	1273, 1793
EcoT38I	GRGCYC	3 cut(s)	326, 558, 1623
ErhI	CCWWGG	3 cut(s)	816, 1139, 1260
Esp3I	CGTCTC	2 cut(s)	117, 1630
FaqI	GGGAC	8 cut(s)	292, 578, 658, 960, 1433, 1697, 1703, 1960
FbaI	TGATCA	2 cut(s)	681, 2220
FblI	GTMKAC	4 cut(s)	126, 717, 826, 2316
Fnu4HI	GCNGC	7 cut(s)	25, 449, 461, 575, 954, 1062, 2120
FokI	GGATG	8 cut(s)	15, 64, 362, 596, 823, 1175, 1838, 2323
FriOI	GRGCYC	3 cut(s)	326, 558, 1623
Fsp4HI	GCNGC	7 cut(s)	25, 449, 461, 575, 954, 1062, 2120
FspBI	CTAG	4 cut(s)	935, 1325, 1577, 1907
GlaI	GCGC	2 cut(s)	403, 447
GluI	GCNGC	7 cut(s)	25, 449, 461, 575, 954, 1062, 2120
GsuI	CTGGAG	2 cut(s)	29, 2321
HaeII	RGCGCY	2 cut(s)	405, 449
HaeIII	GGCC	6 cut(s)	1036, 1283, 1605, 2041, 2169, 2263
HapII	CCGG	6 cut(s)	307, 316, 1594, 1637, 1777, 1994
HgaI	GACGC	2 cut(s)	217, 223
HhaI	GCGC	2 cut(s)	404, 448
Hin1I	GRCGYC	2 cut(s)	887, 1527
Hin6I	GCGC	2 cut(s)	402, 446
HinP1I	GCGC	2 cut(s)	402, 446
HincII	GTYRAC	1 cut(s)	63
HindII	GTYRAC	1 cut(s)	63
HpaI	GTTAAC	1 cut(s)	63
HpaII	CCGG	6 cut(s)	307, 316, 1594, 1637, 1777, 1994
HphI	GGTGA	4 cut(s)	146, 331, 512, 641
Hpy166II	GTNNAC	9 cut(s)	63, 127, 369, 718, 827, 919, 1156, 1819, 2317
Hpy8I	GTNNAC	9 cut(s)	63, 127, 369, 718, 827, 919, 1156, 1819, 2317
Hpy99I	CGWCG	1 cut(s)	263
HpyAV	CCTTC	5 cut(s)	266, 641, 1430, 1516, 2176
HpyCH4III	ACNGT	6 cut(s)	58, 831, 1178, 1294, 1811, 1939
HpyCH4IV	ACGT	6 cut(s)	110, 887, 908, 1527, 2178, 2322
HpySE526I	ACGT	6 cut(s)	110, 887, 908, 1527, 2178, 2322
Hsp92I	GRCGYC	2 cut(s)	887, 1527
HspAI	GCGC	2 cut(s)	402, 446
KflI	GGGWCCC	1 cut(s)	1447
Ksp22I	TGATCA	2 cut(s)	681, 2220
KspAI	GTTAAC	1 cut(s)	63
Kzo9I	GATC	8 cut(s)	4, 18, 681, 865, 1632, 1846, 2195, 2220
LmnI	GCTCC	6 cut(s)	329, 355, 553, 1394, 1560, 2116
Lsp1109I	GCAGC	7 cut(s)	36, 435, 447, 586, 940, 1048, 2106
LweI	GCATC	7 cut(s)	586, 708, 979, 1280, 1424, 1710, 1778
MaeI	CTAG	4 cut(s)	935, 1325, 1577, 1907
MaeII	ACGT	6 cut(s)	110, 887, 908, 1527, 2178, 2322
MaeIII	GTNAC	4 cut(s)	1231, 1303, 1657, 1805
MalI	GATC	8 cut(s)	6, 20, 683, 867, 1634, 1848, 2197, 2222
MboI	GATC	8 cut(s)	4, 18, 681, 865, 1632, 1846, 2195, 2220
MfeI	CAATTG	1 cut(s)	334
MflI	RGATCY	3 cut(s)	4, 1632, 1846
MhlI	GDGCHC	4 cut(s)	326, 513, 558, 1623
MlyI	GAGTC	4 cut(s)	640, 787, 1599, 1912
MmeI	TCCRAC	3 cut(s)	342, 413, 788
Mph1103I	ATGCAT	2 cut(s)	1273, 1793
MroXI	GAANNNNTTC	2 cut(s)	150, 1014
MseI	TTAA	7 cut(s)	62, 104, 786, 1028, 1200, 1475, 2243
MslI	CAYNNNNRTG	4 cut(s)	582, 911, 1824, 1976
MspA1I	CMGCKG	2 cut(s)	343, 539
MspCI	CTTAAG	1 cut(s)	2242
MspI	CCGG	6 cut(s)	307, 316, 1594, 1637, 1777, 1994
MspR9I	CCNGG	6 cut(s)	83, 307, 316, 317, 1595, 1637
MunI	CAATTG	1 cut(s)	334
Mva1269I	GAATGC	2 cut(s)	1898, 2280
MvaI	CCWGG	1 cut(s)	83
NciI	CCSGG	5 cut(s)	307, 316, 317, 1595, 1637
NcoI	CCATGG	1 cut(s)	1260
NdeII	GATC	8 cut(s)	4, 18, 681, 865, 1632, 1846, 2195, 2220
NlaIV	GGNNCC	7 cut(s)	1390, 1448, 1449, 1504, 1556, 1569, 1634
NmeAIII	GCCGAG	1 cut(s)	2289
NsiI	ATGCAT	2 cut(s)	1273, 1793
NspI	RCATGY	2 cut(s)	775, 1727
NspV	TTCGAA	2 cut(s)	1166, 1839
OliI	CACNNNNGTG	1 cut(s)	911
PagI	TCATGA	1 cut(s)	1422
PciI	ACATGT	2 cut(s)	771, 1723
PcsI	WCGNNNNNNNCGW	2 cut(s)	267, 1524
PctI	GAATGC	2 cut(s)	1898, 2280
PdmI	GAANNNNTTC	2 cut(s)	150, 1014
PfeI	GAWTC	9 cut(s)	44, 220, 736, 931, 1002, 1010, 1743, 1997, 2094
PflMI	CCANNNNNTGG	2 cut(s)	82, 1551
PkrI	GCNGC	7 cut(s)	26, 450, 462, 576, 955, 1063, 2121
PleI	GAGTC	4 cut(s)	640, 787, 1598, 1911
PpsI	GAGTC	4 cut(s)	640, 787, 1598, 1911
Ppu21I	YACGTR	2 cut(s)	2179, 2323
PpuMI	RGGWCCY	2 cut(s)	1447, 1568
PscI	ACATGT	2 cut(s)	771, 1723
PsiI	TTATAA	1 cut(s)	1289
Psp124BI	GAGCTC	3 cut(s)	326, 558, 1623
Psp5II	RGGWCCY	2 cut(s)	1447, 1568
Psp6I	CCWGG	1 cut(s)	81
PspEI	GGTNACC	1 cut(s)	1805
PspGI	CCWGG	1 cut(s)	81
PspN4I	GGNNCC	7 cut(s)	1390, 1448, 1449, 1504, 1556, 1569, 1634
PspPI	GGNCC	6 cut(s)	1035, 1447, 1568, 1763, 2039, 2261
PspPPI	RGGWCCY	2 cut(s)	1447, 1568
PstI	CTGCAG	2 cut(s)	1066, 2124
PstNI	CAGNNNCTG	2 cut(s)	82, 2128
PsuI	RGATCY	3 cut(s)	4, 1632, 1846
PvuII	CAGCTG	1 cut(s)	343
RsaI	GTAC	3 cut(s)	368, 776, 2234
RsaNI	GTAC	3 cut(s)	367, 775, 2233
RseI	CAYNNNNRTG	4 cut(s)	582, 911, 1824, 1976
SacI	GAGCTC	3 cut(s)	326, 558, 1623
SaqAI	TTAA	7 cut(s)	62, 104, 786, 1028, 1200, 1475, 2243
SatI	GCNGC	7 cut(s)	25, 449, 461, 575, 954, 1062, 2120
Sau3AI	GATC	8 cut(s)	4, 18, 681, 865, 1632, 1846, 2195, 2220
Sau96I	GGNCC	6 cut(s)	1035, 1447, 1568, 1763, 2039, 2261
SchI	GAGTC	4 cut(s)	640, 787, 1599, 1912
ScrFI	CCNGG	6 cut(s)	83, 307, 316, 317, 1595, 1637
SduI	GDGCHC	4 cut(s)	326, 513, 558, 1623
SfaNI	GCATC	7 cut(s)	586, 708, 979, 1280, 1424, 1710, 1778
SfcI	CTRYAG	3 cut(s)	1062, 1983, 2120
SfuI	TTCGAA	2 cut(s)	1166, 1839
SinI	GGWCC	3 cut(s)	1447, 1568, 1763
SmaI	CCCGGG	1 cut(s)	317
SmiMI	CAYNNNNRTG	4 cut(s)	582, 911, 1824, 1976
SmlI	CTYRAG	2 cut(s)	1394, 2242
SmoI	CTYRAG	2 cut(s)	1394, 2242
SsiI	CCGC	4 cut(s)	22, 347, 537, 822
SspMI	CTAG	4 cut(s)	935, 1325, 1577, 1907
SstI	GAGCTC	3 cut(s)	326, 558, 1623
StyD4I	CCNGG	6 cut(s)	81, 305, 314, 315, 1593, 1635
StyI	CCWWGG	3 cut(s)	816, 1139, 1260
TaaI	ACNGT	6 cut(s)	58, 831, 1178, 1294, 1811, 1939
TaiI	ACGT	6 cut(s)	113, 890, 911, 1530, 2181, 2325
TaqII	GACCGA	1 cut(s)	1866
TatI	WGTACW	2 cut(s)	366, 774
TfiI	GAWTC	9 cut(s)	44, 220, 736, 931, 1002, 1010, 1743, 1997, 2094
Tru1I	TTAA	7 cut(s)	62, 104, 786, 1028, 1200, 1475, 2243
Tru9I	TTAA	7 cut(s)	62, 104, 786, 1028, 1200, 1475, 2243
TscAI	CASTG	2 cut(s)	1071, 1621
TseI	GCWGC	7 cut(s)	24, 448, 460, 574, 953, 1061, 2119
TspDTI	ATGAA	8 cut(s)	36, 1023, 1439, 1452, 1456, 1861, 1941, 2222
TspGWI	ACGGA	1 cut(s)	1699
TspMI	CCCGGG	1 cut(s)	315
TspRI	CASTG	2 cut(s)	1071, 1621
Van91I	CCANNNNNTGG	2 cut(s)	82, 1551
Vha464I	CTTAAG	1 cut(s)	2242
VpaK11BI	GGWCC	3 cut(s)	1447, 1568, 1763
XagI	CCTNNNNNAGG	1 cut(s)	1575
XapI	RAATTY	5 cut(s)	779, 1182, 1835, 1960, 2251
XceI	RCATGY	2 cut(s)	775, 1727
XcmI	CCANNNNNNNNNTGG	1 cut(s)	340
XmaI	CCCGGG	1 cut(s)	315
XmiI	GTMKAC	4 cut(s)	126, 717, 826, 2316
XmnI	GAANNNNTTC	2 cut(s)	150, 1014
XspI	CTAG	4 cut(s)	935, 1325, 1577, 1907
ZraI	GACGTC	2 cut(s)	888, 1528
Zsp2I	ATGCAT	2 cut(s)	1273, 1793

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.