Rorug02G0552300

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011810
Physical Location & Seq
Reverse (-)
67949171 .. 67971678
22508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0552300.1

Sequence Viewer

Length: 6843 bp
ATGGCGAAGAAGCACGTCAAAGATAACCCCCTGCCGCTCTCTCAGTTCGGCGTCTTGGTAGCTCAATTGGAGTCCATAGTCGCCTCCGCCGCCCAGAAGCCACCGGAGCCCCTCCTCTGCTTCGATCTCCTCTCCGATCTCATCTCCGCCATCGCCGAAGAGCCCAAGGAGTCGATTTTGTTATGGCAAAGGCGATGCGAGGAGGCACTTTATTCACTGCTTGTACTCGGAGCTCGGCGGCCGGTGCGGCATTTGGCTTCAGTTGCAATGGCGAGGGTTATATCCAAGGGAGATAGCATTTCAATATACTCCAGAGCCAGCAGTCTCCAAGGGTTTCTTTCTGATGGGAAGAAGAGCGACCCGCAAAAAGTAGCTGGGGCTGCACAATGCTTAGGAGAATTATATCGATATTTTGGGAGGCGAATCACTTCAGGTTTACTTGAAACGACTATGATTGCCACAAAACTCATCAAGTTTCATGAGGAATTTGTGAGACAAGAGGCTTTATATATGCTTCAAAATGCTTTGGAAGGGTCTGGTGGTAGTGCTGCTGCTTCAGCATACACCGAGGCATTTCGTCTCATCATGCGGTTTGCTGTTGGTGATAAATCATTTCTTGTCAGAATCGCTGCTGCAAGATGTTTGAAGGCATTTGCTATTATTGGAGGACCGGGTTTAGGGGTGGGAGAACTTGACAATTCAGCTTCTTTCTGTGCCAAGGCCCTTGAGGATCCTGTTTCATCTGTCCGTGATGCATTTGCTGAAGCATTGGGTTCGCTGCTTGCTCTTGGAATGAATCCTGATGCACAGGTTCAACCAAGAGGGAAGGGACCTTTTCCTCCAGCAAAGAAACTAGAAGGTGGTTTGCAAAGGCATTTAGCATTGCCTTTCACAAAAGCAAGTGGCGCTCGGTCAAAGGATGTGCGAGTTGGCATAACATTGTCTTGGGTGTTCTTCTTACAGGCAATTCGTTTGAAGTACTTGCATCCAGACAGTGAGCTTCAAAACTATGCAATTCAGGTTATGGACATGCTTCGTGCCGATACTTCTGTTGATGCCTACACTCTGGCTTGTGTTCTCTATATCCTTCGTGTTGGTGTTACCGATCAGATGACAGAACCTACTCAGAGGATTTTTTTGGTTTTTCTTGGACAGCAGCTTATGTCTCCTGATGCCAGTCCTTCCATGAAAATTGCTGGTCTACGTACTGTCTCATATACCCTAAAAACTCTGGGAGAGGTTCCAGCTGAGTTCAAGGAAGTACTTGACAACACTGTTGTTGCAGCAGTATCTCATTCTTCTCAACTTGTACGAATTGAGGCTGCTTTGACATTGCGTGCACTGGCTGAGGTTGATCCCACCTGTGTTGGTGGTTTAATTTCATATGGGGTGACCATGTTGAATGCTCTGAGAGAAAATATATCGTTTGAAAAGGGGAGCACTTTGCAATTAGAGCTTGATTCTTTGCATGGGCAAGCTACTGTGTTAGCAACTCTGGTGTCCATTTCCCCAAAATTACCTCTTGGTTATCCCGCTAGGTTGCCCAAATCAGTTCTTGAAGTTTCAAAGAAAATGCTGACTGAATCTAGTCGGAATCCTCTGGCCGCTACAATTGAAAAAGAAGCTGGGTGGTTACTTTTGTCATCTCTCTTAGCATCCATGCCAAAGGAGGAGCTTGAAGACCAGGTTTTTGATATTCTTTCCTTGTGGGTGTCCCTTTTTACTGGAAACCCACAAAATGAAACTAACCAAACTGGGGATTTGATATCTAGGATTCGCATGTGGTCTGCTGCAATTGATGCTCTTACGTCATTTTTAAGATGCTTTCTTTCCCATGATGCTAAGAATAATCGGATTTTGCTTCAACCTGTGCTTGTATACCTAAGTAGGGCTTTATCCTTTATATCACTGATAGCAGCCAAGGAACTGCCAAATGTGAAGCCTGCTCTGAATATATTTATTATCAGGACACTAATAGCTTATCAGTCTCTTCCTGATCCAATGGCATATAAGAATGAACACCCCCAGATTATTCAAATTTGCACTAGTCCATTCAGAGAGGCTTTTGGATGTGAAGAAAGTTCATGCTTGAGGTTTCTGTTAGACAAAAGAGATGCATGGTTGGGTCCCTGGATTCCTGGAAGGGATTGGTTTGAAGATGAACTTCGTGCTTTTCAAGGTGGAAAAGATGGTATTATGCCCTGTGTCTGGGAGAACGAAGTTTCTAGCTTTCCTCAGCCGGAACCAGTAAACAAGATATTGGTGAACCAGATGCTTCTCTGCTTTGGAGTCATGTTTGCTTCTCAGGATAGTGGTGGGATGCTGTCACTTCTTGGAATGATTGAGCAATCTATGAAAGCTGGAAGAAAGCAACCATGGCATGCAGCAAGTGTAACCAATATATGTGTGGGGTTACTTTCAGGATTCAAGGCTTTGCTTTCCTTGCGTTCCCAGCCATTAGCAGTTGATGTATTAAATTCAGCACAGGCTATATTTCAGAGTATTCTAGCAGAGGGTGACATCTGTCCATCACAACGTAGGGCTGCATCAGAATGTCTTGGCCTTTTAGCTCGTCTTGGAAATGATATCTTTACAGCAAGAATGACAAGATCACTGCTTGGTGACCTAACTGGAGCAACAGATTCAAACTATGCAGGTTCAATTGCTTTTGCTCTGGGTTGCATTCATTGCAGTGCCGGAGGAATGGCATTGTCAACATTAGTGCCTTCAACTGTGAGCTCAATTTCTTTGCTTGCTAAAAGTTCAATAGCTGGTTTACAGATCTGGTCTTTACATGGACTTCTGTTAACCATAGAAGCTGCTGGCTTGTCCTATGTATCTCAAGTTCAGGCAACACTTGGCCTTGCTTTGGATATTCTTTTGTCCGAGGAGAATGGTTGGGTTGTACTTCAGCAAGGGGTTGGCCGTCTTATAAATGCTATTGTAGCTGTTCTTGGTCCTGAACTTGCCCCTGGAAGTATTTTCTTTTCACGTTGCAAGTCAGTTATTTCAGAGATAAGCTCAGGGCAAGAAACAGCAACAATGCTCGAGAGTGTTCGCTTTACTCAACAACTTGTACTATTTGCACCACAAGCTGTTTCTGTGCACACCCATGTCCAAACTCTTCTTCCTACTTTATCCTCAAGACAGCCAGTTTTGAGGCATCTTGCTGTTTCCACTCTTCGGCATCTTATTGAGAAAGATCCAGTGTCCGTTGTTAACGAGCAAATAGAAGATAAACTATTTCAAATGCTGGATGAAGAAACTGACTCCGAGATTGGTGATCTGGTGCGAGCCACAATTATGCGATTGCTCTATGCATCAAGCCCTTCACGTCCATCTCATTGGATGTCAATATGTCGAAGTGTGGTTCTTGCCGCATCAATGAGGAGAAATGCTGATAGTATAAACGGTTTAGAAAATGATCCGGTGGGTACAGAGGGTGATCCAAGTTTAAATTCTGGAGAAGATGATGATAACATGGTTTCTGGATCTAGAGGCTCACCCCAGTTGATTCTCAGCAGGGAAAAGCACCTCAGATATCGAACCAGAGTATTTGCTGCTGAGTGTTTGAGTTACCTACCTGGAGCTGTTGGAAAGAATCCAGCTCATTTTGATCTCTGTTTGGCTAGGGACCAATCTACTAATGGACAGGCCTCTGGTGATTGGCTTGTCCTCCATATCCAAGAACTTATAGCACTTGCTTATCAGATAAGCACGATCCAGTTTGAAAACATGCAGCCAATTGGTGTAGTACTTCTGAGTACGATTACAGACAAGTTCGAGAGGACTCCTGACCCGGAGCTTCCAGGGCACCTCCTGCTGGAACAATATCAGGCGCAACTAGTGTCTGCTGTCCGCACTGCCTTAGACTCGTCCTCTGGTCCTATTCTTTTGGAAGCAGGCTTCCAGTTGGCTACAAAGATATTCACAAGCGGAATAATTAAGGGTGATCAGATTGCTGTTAAGCGCATATATTCTTTGATTTCACGACCATTGAATGACTTCAAGGACCTGTATTATCCTTCATTTGCAGAATGGGTCTCGTGCAAGATTAAGATAAGACTTCTTGCTGCCCATGCCTCTCTCAAATGTTATACATATGCATTCTTGAGGAGACACCAAAGTGCGGTTCCAGATGAATATTTAGCACTTTTACCATTATTTTCAAAGAGTTCAGACATGCTTGGGAAGTACTGGATTAGAGTCTTGAAGGACTACAGCTATATTTGCTTGTGCGTGCATCTTAAGGCAAAGTGGAATCCTTTTCTTGATGGAATTCAATCACCTCTGGTATCATCAAAGTTGCAGCCCTGCTTGGAAGAATCCTGGCCAGTAATATTGCTGGCAATTGCATTGGATGCAGTTCCAGTGAATCTTGAAGAGAATGAATCTTCAAAATCCACTGATGAAACTACATCAAGTAATAGCTTATTGTCTGGATATAGTATGGTTCAATTGGAATCCGAAGATTATCAGTTTCTCTGGGGATTTGCTTTGCTTGTTTTATTTCAGGGGCAAAATTCGACCCCCAATGGATTGAAAAATCCAGTTTCTATAGTTAAAGCTTATAACAGGGGAGATCCATCCAGTGAAGAATTATCTTCCCCAGGAGTTAATTTATACGAGATTGTATTACCGGTCTTCCAGTTTTTGTCCACCAAAAGATTTGCCAGTGCAGGATTTCTCACTATGGACATATGCAGTGAATTGCTTCAGGTTTTCTCATATTCTATGTGCATGGATAATTCATGGGATACTCTTTCAGTTTCTGTTCTATCACAGATTGTGAAGAACTGCCCTGAAACGTTTTATGAACTGGAAAACTTTGCTTATCTGGCAATGGAACTCTGTTTGGCGTACCTCTGCAAGGTGTTTCTAAGTACCGATGTGACATCTCTGGACAAATCCTTGGAGGATTTGATATCCTCAATATTTGTCACAGCAAAAACCCTTGTGAACTGTTTTCAACCAAAGAAGCAACTAGTATCTGCGGCATTGGCGTTTCTTTTAATTGGTTACAAGGGAATCAGAGAAGTCTCAACTGACTTCTGCTTCTCCAAACTTGATGAATATTTCAAGTGCACGAGCCTCTTATTGAAAAGATTTATTGATGATACATGCAGTGTTGGTGATGATGGTATTCTTCAGATGAGAAAAATATTGGGAACTTGTCTGAATGTGATCACTGATTTGACCAAGGACTGCAATAAGTGTATTCATATGCTGGAGAATAAGAGATCTGATTCGCATACATTACTGCAAACAAAGCTTGCTTTCTCTCTTGAACAAACTATATCATTTGCCAAGCTGGGTTATGAAATGGATTATCTTGAAGATAATACAGATGGTGATTCAATTTATTATGGTATGTTTAAATATTGCACCAGATGTGTCCAAACTGTACTCACCGATTCAAGTTTACAGGTTCAAGAAATTGGGTTGCAAGTACTAAAAAACTTGATTCAGAAAGGCACAGATGCAGAGGATGACACTTTCTTAATGCTTTTTGTTGGGGAACTTGCTTGTGATTTTTTCCTGATAATGCAGAATATGCTAAAGAAACCTGTAACTGAAAAATCAGCATCTGTTGCTGGTGAATGTTTAAGACTTTTAGTGCTTCTGCAAACATCGTCAAAATCTAGCGAATGTCAGAGAGGTTTTATGAATTTGCTCCTGGAAGCTGTTATCGTGGTTTTCAAGGCTTCAGAAGAAGGTTCTTCTCAGGAAGTCAATAAACTTAGGAGCATTGCTGTGAGACTTGTTTCTCATCTTGCTCAAGTTCCTTCCTCTGCAGTTCACTTCAAGGATGTGTTGCTGTCTATGCCTCTGACTCACCGACAGCAATTTCAGGGGTTTATTCGTGCTTCTGTGACCCAAGAGCATAATGCAACACAGATGAAGCCCACAACTCCATTTTTGGAGATAAAACTACCAGTGCCAACAGTGGTAAGTAAAGAGAAACAGTATCCACTGCCAGCCACTACATCACATTCTTCTGTTTCTGATTATCAAAGAGATGAGGAGGAGGAAGAGGATGAAGATGATTGGGATGCTTTTCAGTCTTTTCCTGCTACTACAAACGCAGCTGAAATTGACTCGAGAGTAGACAGTGCCTTGGAAACACCCGACCTGGTTGAGAAATCGTCTCCTTCGGAAGTGAATACAGAGAGTGATCAATTTCATGGAGATTCTGTTTCCCAACCTCTCAACAATGTAGAGGCTACCAGCAAGGCAGACCATCAAGAGGCTGGCATAGCTGAGGTGATATCTGATTCCCCAGATGATCTGACATCCTCACAGGGTAATATACTCTGTGACAATGTCAAGACAGAAAAACCCGATGATTCCCGAACTAATAGTGGTGTTATTGAGGCATTGGATGATCAAAAGGAGAGAGACGATAAATTGTTATCCGGACCAGAAGAGGGAAAGGGAGCAGAGTTGAACCAAGTTACTGAACACAGAACATCTGAACTTCATCCTATTGAAGATGCTGAAGGATTGGTCGGAAAGAATTCAGCACACCATGAGCAGGGAAAGGAGAACACTGATAATAGACCAGTGCAATCATCATTAGATCCTGTAAAGCTTGGTGAGGAAGTAAAGGGAGGAGGGTCGAACCAAGTGACAGAACAGATACCATCTGAACTTCATCCTGTCGAAGATGCTCAAGGATCGGTTGCAGTGAATTCAGCGCACCATGAGCAGGGAAAGGAGATCCCTGATAATAGATCAGTGCAATCATCATCAGATCCAGTCAAGCTTGACCAGGAAGTAAAGGATAAAAGAGAAGACAAAGAAGAGTCGGCAGTTGAAGATTCTCGGCCCCAACAACACTAA

Protein Analysis

2280

Amino Acids

250.42

Weight (kDa)

5.18

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Laa1_Sip1_HTR5 PF20210 1223 - 1408 3.6e-43 Laa1/Sip1/HEATR5 HEAT repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011810)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14880
fragaria_vesca FvH4_6g50500 FvH4_6g50500
malus_domestica MD09G1035900.v1.1 MD17G1037500.v1.1
prunus_persica Prupe.3G281900_v2.0.a1 Prupe.3G281900_v2.0.a1
pyrus_communis pycom111g02870 pycom17g03360
rosa_chinensis RchiOBHm_Chr2g0170921
rosa_laevigata RLG00000021992
rosa_multiflora Rmu_sc0009938.1_g000001
rosa_roxburghii Rroxscaffold_2G00080980
rosa_rugosa Rorug02G0552300
rosa_samantha Rh2AG625000 Rh2BG635700 Rh2CG604400 Rh2DG647300
rosa_wichuraiana Rw2G051770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2933, 4572
Acc36I ACCTGC 1 cut(s) 2645
AccB1I GGYRCC 1 cut(s) 3813
AccBSI CCGCTC 1 cut(s) 37
AccI GTMKAC 3 cut(s) 1201, 1878, 6108
AccIII TCCGGA 1 cut(s) 6416
AclI AACGTT 1 cut(s) 4808
AcoI YGGCCR 4 cut(s) 239, 1602, 2923, 4331
AcsI RAATTY 9 cut(s) 485, 2037, 2476, 3457, 4278, 4522, 5668, 6517, 6691
AcuI CTGAAG 9 cut(s) 243, 414, 540, 783, 2894, 4700, 5132, 5691, 6519
AcyI GRCGYC 1 cut(s) 51
AflII CTTAAG 1 cut(s) 4247
AgeI ACCGGT 1 cut(s) 4639
AhlI ACTAGT 3 cut(s) 2045, 3844, 4984
AjiI CACGTC 2 cut(s) 16, 3335
AjuI GAANNNNNNNTTGG 6 cut(s) 3111, 3143, 3442, 3474, 4116, 4148
AleI CACNNNNGTG 1 cut(s) 4125
Alw21I GWGCWC 6 cut(s) 235, 1342, 1442, 2741, 3108, 5087
Alw44I GTGCAC 3 cut(s) 1338, 3104, 5083
AlwNI CAGNNNCTG 2 cut(s) 4772, 5588
Ama87I CYCGRG 2 cut(s) 3047, 6100
Aor13HI TCCGGA 1 cut(s) 6416
AoxI GGCC 9 cut(s) 239, 720, 1602, 2560, 2860, 2923, 3654, 4331, 6827
ApaLI GTGCAC 3 cut(s) 1338, 3104, 5083
ApoI RAATTY 9 cut(s) 485, 2037, 2476, 3457, 4278, 4522, 5668, 6517, 6691
ArsI GACNNNNNNTTYG 2 cut(s) 1673, 1705
AsiGI ACCGGT 1 cut(s) 4639
Asp700I GAANNNNTTC 4 cut(s) 427, 4004, 4713, 6517
AspLEI GCGC 4 cut(s) 908, 3841, 3972, 6700
AsuC2I CCSGG 2 cut(s) 672, 3800
AvaI CYCGRG 2 cut(s) 3047, 6100
AvaII GGWCC 8 cut(s) 668, 830, 2126, 2957, 3634, 3884, 4012, 6419
BaeGI GKGCMC 4 cut(s) 1342, 3108, 3816, 5087
BaeI ACNNNNGTAYC 2 cut(s) 1035, 1068
BalI TGGCCA 1 cut(s) 4333
BamHI GGATCC 1 cut(s) 730
BanI GGYRCC 1 cut(s) 3813
BanII GRGCYC 4 cut(s) 111, 165, 235, 2741
BauI CACGAG 2 cut(s) 4045, 5086
BbsI GAAGAC 3 cut(s) 1686, 4636, 6801
Bbv12I GWGCWC 6 cut(s) 235, 1342, 1442, 2741, 3108, 5087
BbvCI CCTCAGC 3 cut(s) 1347, 2235, 6261
BceAI ACGGC 1 cut(s) 2910
BcgI CGANNNNNNTGC 2 cut(s) 756, 790
BciVI GTATCC 2 cut(s) 4750, 5979
BclI TGATCA 4 cut(s) 3952, 5184, 6175, 6385
BcnI CCSGG 2 cut(s) 672, 3800
BcuI ACTAGT 3 cut(s) 2045, 3844, 4984
BfmI CTRYAG 3 cut(s) 4219, 4557, 5793
BfoI RGCGCY 1 cut(s) 909
BfrI CTTAAG 1 cut(s) 4247
BfuAI ACCTGC 1 cut(s) 2645
BfuI GTATCC 2 cut(s) 4750, 5979
BglI GCCNNNNNGGC 1 cut(s) 247
BglII AGATCT 2 cut(s) 2781, 5240
BmcAI AGTACT 5 cut(s) 980, 1263, 3756, 4196, 5451
Bme18I GGWCC 8 cut(s) 668, 830, 2126, 2957, 3634, 3884, 4012, 6419
BmeT110I CYCGRG 2 cut(s) 3047, 6100
BmgBI CACGTC 2 cut(s) 16, 3335
BmrI ACTGGG 2 cut(s) 1764, 3502
BmuI ACTGGG 2 cut(s) 1764, 3502
BoxI GACNNNNGTC 1 cut(s) 2523
BpiI GAAGAC 3 cut(s) 1686, 4636, 6801
BplI GAGNNNNNCTC 2 cut(s) 3005, 3037
BpmI CTGGAG 6 cut(s) 295, 825, 2652, 3483, 3606, 5249
Bpu10I CCTNAGC 5 cut(s) 391, 1347, 2235, 3022, 6261
BpuEI CTTGAG 7 cut(s) 746, 2110, 2826, 3127, 4132, 5763, 6657
BpuMI CCSGG 2 cut(s) 672, 3800
Bsa29I ATCGAT 1 cut(s) 406
BsaAI YACGTR 1 cut(s) 1205
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 4048
BsaWI WCCGGW 4 cut(s) 103, 3427, 4639, 6416
BsaXI ACNNNNNCTCC 2 cut(s) 6180, 6210
Bse118I RCCGGY 2 cut(s) 241, 4639
Bse3DI GCAATG 6 cut(s) 273, 881, 1331, 2686, 4848, 5748
BseAI TCCGGA 1 cut(s) 6416
BseCI ATCGAT 1 cut(s) 406
BseMI GCAATG 6 cut(s) 273, 881, 1331, 2686, 4848, 5748
BseSI GKGCMC 4 cut(s) 1342, 3108, 3816, 5087
BseX3I CGGCCG 1 cut(s) 239
BseYI CCCAGC 4 cut(s) 374, 1625, 2451, 5311
BsgI GTGCAG 2 cut(s) 366, 4698
Bsh1285I CGRYCG 1 cut(s) 242
BshFI GGCC 9 cut(s) 241, 722, 1604, 2562, 2862, 2925, 3656, 4333, 6829
BshNI GGYRCC 1 cut(s) 3813
BshTI ACCGGT 1 cut(s) 4639
BshVI ATCGAT 1 cut(s) 406
BsiEI CGRYCG 1 cut(s) 242
BsiHKAI GWGCWC 6 cut(s) 235, 1342, 1442, 2741, 3108, 5087
BsiHKCI CYCGRG 2 cut(s) 3047, 6100
BsiSI CCGG 9 cut(s) 104, 242, 671, 2240, 2697, 3428, 3800, 4640, 6417
BslFI GGGAC 4 cut(s) 843, 1699, 2112, 3647
BsmBI CGTCTC 3 cut(s) 584, 6153, 6393
BsmFI GGGAC 4 cut(s) 843, 1699, 2112, 3647
BsmI GAATGC 3 cut(s) 1408, 2682, 4106
BsnI GGCC 9 cut(s) 241, 722, 1604, 2562, 2862, 2925, 3656, 4333, 6829
Bso31I GGTCTC 1 cut(s) 4048
BsoBI CYCGRG 2 cut(s) 3047, 6100
Bsp1286I GDGCHC 9 cut(s) 111, 165, 235, 1342, 1442, 2741, 3108, 3816, 5087
Bsp13I TCCGGA 1 cut(s) 6416
Bsp19I CCATGG 1 cut(s) 2375
BspANI GGCC 9 cut(s) 241, 722, 1604, 2562, 2862, 2925, 3656, 4333, 6829
BspDI ATCGAT 1 cut(s) 406
BspEI TCCGGA 1 cut(s) 6416
BspHI TCATGA 1 cut(s) 478
BspMAI CTGCAG 1 cut(s) 5797
BspMI ACCTGC 1 cut(s) 2645
BspQI GCTCTTC 2 cut(s) 153, 347
BspT107I GGYRCC 1 cut(s) 3813
BspTI CTTAAG 1 cut(s) 4247
BspTNI GGTCTC 1 cut(s) 4048
BsrBI CCGCTC 1 cut(s) 37
BsrDI GCAATG 6 cut(s) 273, 881, 1331, 2686, 4848, 5748
BsrFI RCCGGY 2 cut(s) 241, 4639
BssAI RCCGGY 2 cut(s) 241, 4639
BssNAI GTATAC 1 cut(s) 1879
BssNI GRCGYC 1 cut(s) 51
BssSI CACGAG 2 cut(s) 4045, 5086
BssT1I CCWWGG 9 cut(s) 165, 285, 328, 717, 1920, 2375, 4911, 5199, 6117
Bst1107I GTATAC 1 cut(s) 1879
Bst2BI CACGAG 2 cut(s) 4045, 5086
Bst6I CTCTTC 9 cut(s) 153, 347, 1995, 3129, 3186, 4377, 6027, 6420, 6798
BstACI GRCGYC 1 cut(s) 51
BstAFI CTTAAG 1 cut(s) 4247
BstAPI GCANNNNNTGC 6 cut(s) 1799, 2688, 3820, 4361, 5554, 5591
BstBAI YACGTR 1 cut(s) 1205
BstDSI CCRYGG 1 cut(s) 2375
BstEII GGTNACC 2 cut(s) 1390, 2621
BstENI CCTNNNNNAGG 1 cut(s) 4868
BstH2I RGCGCY 1 cut(s) 909
BstHHI GCGC 4 cut(s) 908, 3841, 3972, 6700
BstMCI CGRYCG 1 cut(s) 242
BstNSI RCATGY 6 cut(s) 1033, 1783, 2384, 3739, 4186, 5124
BstPAI GACNNNNGTC 1 cut(s) 2523
BstPI GGTNACC 2 cut(s) 1390, 2621
BstSFI CTRYAG 3 cut(s) 4219, 4557, 5793
BstSLI GKGCMC 4 cut(s) 1342, 3108, 3816, 5087
BstSNI TACGTA 1 cut(s) 1205
BstV2I GAAGAC 3 cut(s) 1686, 4636, 6801
BstX2I RGATCY 9 cut(s) 730, 2781, 3202, 3491, 4582, 5240, 6580, 6720, 6754
BstXI CCANNNNNNTGG 1 cut(s) 3345
BstYI RGATCY 9 cut(s) 730, 2781, 3202, 3491, 4582, 5240, 6580, 6720, 6754
BstZ17I GTATAC 1 cut(s) 1879
BstZI CGGCCG 1 cut(s) 239
Bsu15I ATCGAT 1 cut(s) 406
BsuI GTATCC 2 cut(s) 4750, 5979
BsuRI GGCC 9 cut(s) 241, 722, 1604, 2562, 2862, 2925, 3656, 4333, 6829
BsuTUI ATCGAT 1 cut(s) 406
BtgI CCRYGG 1 cut(s) 2375
BtgZI GCGATG 2 cut(s) 136, 208
BtrI CACGTC 2 cut(s) 16, 3335
BtsI GCAGTG 8 cut(s) 215, 2612, 2698, 3861, 4711, 5131, 5972, 6693
BveI ACCTGC 1 cut(s) 2645
CaiI CAGNNNCTG 2 cut(s) 4772, 5588
CciI TCATGA 1 cut(s) 478
CfoI GCGC 4 cut(s) 908, 3841, 3972, 6700
Cfr10I RCCGGY 2 cut(s) 241, 4639
ClaI ATCGAT 1 cut(s) 406
CseI GACGC 1 cut(s) 40
CsiI ACCWGGT 2 cut(s) 1683, 6132
CspAI ACCGGT 1 cut(s) 4639
CspCI CAANNNNNGTGG 2 cut(s) 1348, 1383
DraI TTTAAA 2 cut(s) 3456, 5377
EaeI YGGCCR 4 cut(s) 239, 1602, 2923, 4331
EagI CGGCCG 1 cut(s) 239
Eam1104I CTCTTC 9 cut(s) 153, 347, 1995, 3129, 3186, 4377, 6027, 6420, 6798
EarI CTCTTC 9 cut(s) 153, 347, 1995, 3129, 3186, 4377, 6027, 6420, 6798
EciI GGCGGA 2 cut(s) 76, 136
Ecl136II GAGCTC 2 cut(s) 233, 2739
EclXI CGGCCG 1 cut(s) 239
Eco105I TACGTA 1 cut(s) 1205
Eco130I CCWWGG 9 cut(s) 165, 285, 328, 717, 1920, 2375, 4911, 5199, 6117
Eco147I AGGCCT 1 cut(s) 3656
Eco24I GRGCYC 4 cut(s) 111, 165, 235, 2741
Eco31I GGTCTC 1 cut(s) 4048
Eco32I GATATC 5 cut(s) 1767, 2587, 3542, 4926, 6270
Eco47I GGWCC 8 cut(s) 668, 830, 2126, 2957, 3634, 3884, 4012, 6419
Eco52I CGGCCG 1 cut(s) 239
Eco53kI GAGCTC 2 cut(s) 233, 2739
Eco57I CTGAAG 9 cut(s) 243, 414, 540, 783, 2894, 4700, 5132, 5691, 6519
Eco88I CYCGRG 2 cut(s) 3047, 6100
Eco91I GGTNACC 2 cut(s) 1390, 2621
EcoICRI GAGCTC 2 cut(s) 233, 2739
EcoNI CCTNNNNNAGG 1 cut(s) 4868
EcoO109I RGGNCCY 4 cut(s) 721, 830, 2126, 4012
EcoO65I GGTNACC 2 cut(s) 1390, 2621
EcoRI GAATTC 3 cut(s) 4278, 6517, 6691
EcoRV GATATC 5 cut(s) 1767, 2587, 3542, 4926, 6270
EcoT14I CCWWGG 9 cut(s) 165, 285, 328, 717, 1920, 2375, 4911, 5199, 6117
EcoT22I ATGCAT 4 cut(s) 757, 2119, 3322, 4108
EcoT38I GRGCYC 4 cut(s) 111, 165, 235, 2741
ErhI CCWWGG 9 cut(s) 165, 285, 328, 717, 1920, 2375, 4911, 5199, 6117
Esp3I CGTCTC 3 cut(s) 584, 6153, 6393
FalI AAGNNNNNCTT 6 cut(s) 321, 353, 1876, 1908, 2148, 2180
FaqI GGGAC 4 cut(s) 843, 1699, 2112, 3647
FauI CCCGC 2 cut(s) 369, 1540
FauNDI CATATG 4 cut(s) 1384, 4102, 4700, 5223
FbaI TGATCA 4 cut(s) 3952, 5184, 6175, 6385
FblI GTMKAC 3 cut(s) 1201, 1878, 6108
FriOI GRGCYC 4 cut(s) 111, 165, 235, 2741
GlaI GCGC 4 cut(s) 907, 3840, 3971, 6699
GsaI CCCAGC 4 cut(s) 378, 1629, 2455, 5315
GsuI CTGGAG 6 cut(s) 295, 825, 2652, 3483, 3606, 5249
HaeII RGCGCY 1 cut(s) 909
HaeIII GGCC 9 cut(s) 241, 722, 1604, 2562, 2862, 2925, 3656, 4333, 6829
HapII CCGG 9 cut(s) 104, 242, 671, 2240, 2697, 3428, 3800, 4640, 6417
HgaI GACGC 1 cut(s) 40
HhaI GCGC 4 cut(s) 908, 3841, 3972, 6700
Hin1I GRCGYC 1 cut(s) 51
Hin6I GCGC 4 cut(s) 906, 3839, 3970, 6698
HinP1I GCGC 4 cut(s) 906, 3839, 3970, 6698
HincII GTYRAC 3 cut(s) 2715, 2808, 3220
HindII GTYRAC 3 cut(s) 2715, 2808, 3220
HindIII AAGCTT 4 cut(s) 4566, 5270, 6590, 6764
HpaI GTTAAC 2 cut(s) 2808, 3220
HpaII CCGG 9 cut(s) 104, 242, 671, 2240, 2697, 3428, 3800, 4640, 6417
HpyCH4IV ACGT 7 cut(s) 15, 1204, 1808, 2536, 2992, 3334, 4808
HpySE526I ACGT 7 cut(s) 15, 1204, 1808, 2536, 2992, 3334, 4808
Hsp92I GRCGYC 1 cut(s) 51
HspAI GCGC 4 cut(s) 906, 3839, 3970, 6698
KflI GGGWCCC 1 cut(s) 2126
Kpn2I TCCGGA 1 cut(s) 6416
Ksp22I TGATCA 4 cut(s) 3952, 5184, 6175, 6385
KspAI GTTAAC 2 cut(s) 2808, 3220
LguI GCTCTTC 2 cut(s) 153, 347
MabI ACCWGGT 2 cut(s) 1683, 6132
MaeII ACGT 7 cut(s) 15, 1204, 1808, 2536, 2992, 3334, 4808
MbiI CCGCTC 1 cut(s) 37
MfeI CAATTG 7 cut(s) 65, 1611, 1794, 2661, 3744, 4350, 4457
MflI RGATCY 9 cut(s) 730, 2781, 3202, 3491, 4582, 5240, 6580, 6720, 6754
MhlI GDGCHC 9 cut(s) 111, 165, 235, 1342, 1442, 2741, 3108, 3816, 5087
MlsI TGGCCA 1 cut(s) 4333
MluNI TGGCCA 1 cut(s) 4333
MmeI TCCRAC 3 cut(s) 1571, 3574, 6490
Mox20I TGGCCA 1 cut(s) 4333
Mph1103I ATGCAT 4 cut(s) 757, 2119, 3322, 4108
MroI TCCGGA 1 cut(s) 6416
MroXI GAANNNNTTC 4 cut(s) 427, 4004, 4713, 6517
MscI TGGCCA 1 cut(s) 4333
MslI CAYNNNNRTG 6 cut(s) 2551, 2691, 3111, 3302, 4125, 5753
Msp20I TGGCCA 1 cut(s) 4333
MspA1I CMGCKG 2 cut(s) 1247, 6089
MspCI CTTAAG 1 cut(s) 4247
MspI CCGG 9 cut(s) 104, 242, 671, 2240, 2697, 3428, 3800, 4640, 6417
MunI CAATTG 7 cut(s) 65, 1611, 1794, 2661, 3744, 4350, 4457
Mva1269I GAATGC 3 cut(s) 1408, 2682, 4106
NciI CCSGG 2 cut(s) 672, 3800
NcoI CCATGG 1 cut(s) 2375
NdeI CATATG 4 cut(s) 1384, 4102, 4700, 5223
NmeAIII GCCGAG 2 cut(s) 214, 6805
NmuCI GTSAC 9 cut(s) 1390, 2325, 2516, 2621, 4891, 4939, 5872, 6317, 6628
NsiI ATGCAT 4 cut(s) 757, 2119, 3322, 4108
NspI RCATGY 6 cut(s) 1033, 1783, 2384, 3739, 4186, 5124
OliI CACNNNNGTG 1 cut(s) 4125
PaeI GCATGC 1 cut(s) 2384
PaeR7I CTCGAG 2 cut(s) 3047, 6100
PagI TCATGA 1 cut(s) 478
PceI AGGCCT 1 cut(s) 3656
PciSI GCTCTTC 2 cut(s) 153, 347
PctI GAATGC 3 cut(s) 1408, 2682, 4106
PdmI GAANNNNTTC 4 cut(s) 427, 4004, 4713, 6517
PflFI GACNNNGTC 1 cut(s) 6323
PfoI TCCNGGA 2 cut(s) 2137, 5676
PinAI ACCGGT 1 cut(s) 4639
Ppu21I YACGTR 1 cut(s) 1205
PpuMI RGGWCCY 3 cut(s) 830, 2126, 4012
PshAI GACNNNNGTC 1 cut(s) 2523
PsiI TTATAA 2 cut(s) 2933, 4572
Psp124BI GAGCTC 2 cut(s) 235, 2741
Psp1406I AACGTT 1 cut(s) 4808
Psp5II RGGWCCY 3 cut(s) 830, 2126, 4012
PspEI GGTNACC 2 cut(s) 1390, 2621
PspFI CCCAGC 4 cut(s) 374, 1625, 2451, 5311
PspPPI RGGWCCY 3 cut(s) 830, 2126, 4012
PsrI GAACNNNNNNTAC 2 cut(s) 2829, 2861
PstI CTGCAG 1 cut(s) 5797
PstNI CAGNNNCTG 2 cut(s) 4772, 5588
PsuI RGATCY 9 cut(s) 730, 2781, 3202, 3491, 4582, 5240, 6580, 6720, 6754
PsyI GACNNNGTC 1 cut(s) 6323
PvuII CAGCTG 2 cut(s) 1247, 6089
RseI CAYNNNNRTG 6 cut(s) 2551, 2691, 3111, 3302, 4125, 5753
SacI GAGCTC 2 cut(s) 235, 2741
SapI GCTCTTC 2 cut(s) 153, 347
ScaI AGTACT 5 cut(s) 980, 1263, 3756, 4196, 5451
SduI GDGCHC 9 cut(s) 111, 165, 235, 1342, 1442, 2741, 3108, 3816, 5087
SexAI ACCWGGT 2 cut(s) 1683, 6132
SfcI CTRYAG 3 cut(s) 4219, 4557, 5793
Sfr274I CTCGAG 2 cut(s) 3047, 6100
SinI GGWCC 8 cut(s) 668, 830, 2126, 2957, 3634, 3884, 4012, 6419
SlaI CTCGAG 2 cut(s) 3047, 6100
SmiMI CAYNNNNRTG 6 cut(s) 2551, 2691, 3111, 3302, 4125, 5753
SnaBI TACGTA 1 cut(s) 1205
SpeI ACTAGT 3 cut(s) 2045, 3844, 4984
SphI GCATGC 1 cut(s) 2384
SseBI AGGCCT 1 cut(s) 3656
SspI AATATT 6 cut(s) 4145, 4341, 4935, 5075, 5163, 5381
SstI GAGCTC 2 cut(s) 235, 2741
StuI AGGCCT 1 cut(s) 3656
StyI CCWWGG 9 cut(s) 165, 285, 328, 717, 1920, 2375, 4911, 5199, 6117
TaiI ACGT 7 cut(s) 18, 1207, 1811, 2539, 2995, 3337, 4811
TaqII GACCGA 1 cut(s) 900
TatI WGTACW 9 cut(s) 223, 978, 1261, 2905, 3076, 3754, 4194, 5404, 5449
TauI GCSGC 7 cut(s) 37, 92, 241, 250, 1607, 3380, 4997
TseFI GTSAC 9 cut(s) 1390, 2325, 2516, 2621, 4891, 4939, 5872, 6317, 6628
Tsp45I GTSAC 9 cut(s) 1390, 2325, 2516, 2621, 4891, 4939, 5872, 6317, 6628
TspGWI ACGGA 2 cut(s) 737, 3202
Tth111I GACNNNGTC 1 cut(s) 6323
Vha464I CTTAAG 1 cut(s) 4247
VneI GTGCAC 3 cut(s) 1338, 3104, 5083
VpaK11BI GGWCC 8 cut(s) 668, 830, 2126, 2957, 3634, 3884, 4012, 6419
XagI CCTNNNNNAGG 1 cut(s) 4868
XapI RAATTY 9 cut(s) 485, 2037, 2476, 3457, 4278, 4522, 5668, 6517, 6691
XbaI TCTAGA 1 cut(s) 3494
XceI RCATGY 6 cut(s) 1033, 1783, 2384, 3739, 4186, 5124
XcmI CCANNNNNNNNNTGG 3 cut(s) 2404, 3644, 6248
XhoI CTCGAG 2 cut(s) 3047, 6100
XmiI GTMKAC 3 cut(s) 1201, 1878, 6108
XmnI GAANNNNTTC 4 cut(s) 427, 4004, 4713, 6517
ZrmI AGTACT 5 cut(s) 980, 1263, 3756, 4196, 5451
Zsp2I ATGCAT 4 cut(s) 757, 2119, 3322, 4108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.