MD09G1057200.v1.1

Cell division topological specificity factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0016378
Physical Location & Seq
Reverse (-)
3777284 .. 3778891
1608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1057200.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGCTTTTTCTGGTGATTTTGGAATCTCTGCAATTTTTGGAACTTTCCCTTTTAAGCACCACCACTCTCTGGGAATCTCTCTCCAACCCTCCAAGGTAAAATATAACGGTTTCAATGGCGGAGGTAGTTTTCCTCTGATGAATCAAACGTCTTTCTCAAATCTAGTTTGCTATAGCTCGAACTCGAAGTGTCACTATGACCAATCATTTGGAATTGAAGGAAACAGTGAACTCTTTCCGGGACCTATAAGCCAAGAAACAGAAGGCTTTCTCCTCAATGCTATAAACATGAGCTTTTTCGAGCGCTTGAATTTGGCATGTAAGATATTGCTCCCTTCACCATCGACACGAAGGACTTCCAATGCAAAGATTGCAAAGCAGCGGTTGCAGATGATCCTCTTCTCTGATCGATGCACAGTAACTGATGAGGCGAAACAGAAGATTGTAAGCAACATTGTCGGAGCTCTGTCCGATTTTGTGGAAATAGACTCGAAGGATAAAGTCCAGCTTAGTGTCTCGGAGGATCAGGACCTAGGCACCGTGTACTCCGTTACAATCCCCGTTCGGCGTGTGAAGCCCGAATATCAGGACGAGGATGATGTTGAGGGCATATCAAATGGTGAAGACATGGATACAGGGGGCATGCAACAGATTGAAGAGAGAGGGAAAGACAGAGAAGGGGGCATGCAATAG

Protein Analysis

231

Amino Acids

25.4

Weight (kDa)

5.05

Isoelectric Point (pI)

51.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MinE PF03776 122 - 189 3.2e-14 Septum formation topological specificity factor MinE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016378)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g47773
malus_domestica MD09G1057200.v1.1
prunus_persica Prupe.3G261900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0166971
rosa_laevigata RLG00000021685
rosa_multiflora Rmu_sc0002735.1_g000025 Rmu_sc0010966.1_g000001 Rmu_sc0025421.1_g000002
rosa_roxburghii Rroxscaffold_2G00083900
rosa_rugosa Rorug02G0527000
rosa_samantha Rh2AG593900 Rh2BG605400 Rh2CG576300 Rh2DG616900
rosa_wichuraiana Rw2G049450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 536
AccB7I CCANNNNNTGG 1 cut(s) 70
AciI CCGC 2 cut(s) 120, 382
AclWI GGATC 2 cut(s) 388, 531
AcsI RAATTY 1 cut(s) 310
AfaI GTAC 1 cut(s) 545
AfeI AGCGCT 1 cut(s) 305
AfiI CCNNNNNNNGG 1 cut(s) 70
AgsI TTSAA 4 cut(s) 115, 218, 310, 656
AluBI AGCT 4 cut(s) 177, 294, 464, 508
AluI AGCT 4 cut(s) 177, 294, 464, 508
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 1 cut(s) 520
AlwI GGATC 2 cut(s) 388, 531
AlwNI CAGNNNCTG 1 cut(s) 422
Aor51HI AGCGCT 1 cut(s) 305
ApeKI GCWGC 1 cut(s) 379
ApoI RAATTY 1 cut(s) 310
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp700I GAANNNNTTC 3 cut(s) 234, 267, 355
AspA2I CCTAGG 1 cut(s) 532
AspLEI GCGC 1 cut(s) 306
AspS9I GGNCC 2 cut(s) 242, 529
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 3 cut(s) 26, 330, 632
AvaII GGWCC 2 cut(s) 242, 529
AvrII CCTAGG 1 cut(s) 532
BanI GGYRCC 1 cut(s) 536
BanII GRGCYC 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 1 cut(s) 391
BccI CCATC 1 cut(s) 349
BcgI CGANNNNNNTGC 2 cut(s) 439, 473
BciVI GTATCC 1 cut(s) 625
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 520
BfaI CTAG 2 cut(s) 164, 533
BfmI CTRYAG 1 cut(s) 172
BfoI RGCGCY 1 cut(s) 307
BfuI GTATCC 1 cut(s) 625
BisI GCNGC 1 cut(s) 380
BlnI CCTAGG 1 cut(s) 532
BlsI GCNGC 1 cut(s) 381
Bme1390I CCNGG 1 cut(s) 240
Bme18I GGWCC 2 cut(s) 242, 529
BmgT120I GGNCC 2 cut(s) 242, 529
BmiI GGNNCC 2 cut(s) 243, 538
BmrFI CCNGG 1 cut(s) 240
BmsI GCATC 1 cut(s) 401
BpiI GAAGAC 1 cut(s) 630
BpuMI CCSGG 1 cut(s) 240
Bsa29I ATCGAT 1 cut(s) 409
BsaJI CCNNGG 2 cut(s) 93, 532
Bsc4I CCNNNNNNNGG 1 cut(s) 70
BseCI ATCGAT 1 cut(s) 409
BseDI CCNNGG 2 cut(s) 93, 532
BseGI GGATG 1 cut(s) 601
BseLI CCNNNNNNNGG 1 cut(s) 70
BseRI GAGGAG 1 cut(s) 263
BseXI GCAGC 1 cut(s) 391
BshNI GGYRCC 1 cut(s) 536
BshVI ATCGAT 1 cut(s) 409
BsiHKAI GWGCWC 1 cut(s) 466
BsiSI CCGG 1 cut(s) 239
BslFI GGGAC 1 cut(s) 255
BslI CCNNNNNNNGG 1 cut(s) 70
BsmAI GTCTC 1 cut(s) 520
BsmFI GGGAC 1 cut(s) 255
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 3 cut(s) 393, 406, 523
BspACI CCGC 2 cut(s) 120, 382
BspDI ATCGAT 1 cut(s) 409
BspLI GGNNCC 2 cut(s) 243, 538
BspPI GGATC 2 cut(s) 388, 531
BspT107I GGYRCC 1 cut(s) 536
BssECI CCNNGG 2 cut(s) 93, 532
BssMI GATC 3 cut(s) 393, 406, 523
BssT1I CCWWGG 2 cut(s) 93, 532
Bst4CI ACNGT 4 cut(s) 110, 227, 418, 541
Bst6I CTCTTC 2 cut(s) 404, 651
BstAPI GCANNNNNTGC 2 cut(s) 371, 385
BstC8I GCNNGC 2 cut(s) 644, 686
BstDEI CTNAG 1 cut(s) 509
BstF5I GGATG 1 cut(s) 601
BstH2I RGCGCY 1 cut(s) 307
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 3 cut(s) 396, 409, 526
BstMAI GTCTC 1 cut(s) 520
BstMBI GATC 3 cut(s) 393, 406, 523
BstMWI GCNNNNNNNGC 3 cut(s) 371, 385, 574
BstNSI RCATGY 3 cut(s) 321, 646, 688
BstSCI CCNGG 1 cut(s) 238
BstSFI CTRYAG 1 cut(s) 172
BstV1I GCAGC 1 cut(s) 391
BstV2I GAAGAC 1 cut(s) 630
BstXI CCANNNNNNTGG 1 cut(s) 209
Bsu15I ATCGAT 1 cut(s) 409
BsuI GTATCC 1 cut(s) 625
BsuTUI ATCGAT 1 cut(s) 409
BtsCI GGATG 1 cut(s) 601
BtsIMutI CAGTG 1 cut(s) 232
Cac8I GCNNGC 2 cut(s) 644, 686
CaiI CAGNNNCTG 1 cut(s) 422
CfoI GCGC 1 cut(s) 306
Cfr13I GGNCC 2 cut(s) 242, 529
ClaI ATCGAT 1 cut(s) 409
Csp6I GTAC 1 cut(s) 544
CviAII CATG 5 cut(s) 289, 318, 628, 643, 685
CviJI RGCY 8 cut(s) 5, 177, 252, 267, 294, 464, 508, 577
CviKI_1 RGCY 8 cut(s) 5, 177, 252, 267, 294, 464, 508, 577
CviQI GTAC 1 cut(s) 544
DdeI CTNAG 1 cut(s) 509
DpnI GATC 3 cut(s) 395, 408, 525
DpnII GATC 3 cut(s) 393, 406, 523
Eam1104I CTCTTC 2 cut(s) 404, 651
EarI CTCTTC 2 cut(s) 404, 651
EciI GGCGGA 1 cut(s) 135
Ecl136II GAGCTC 1 cut(s) 464
Eco130I CCWWGG 2 cut(s) 93, 532
Eco24I GRGCYC 1 cut(s) 466
Eco47I GGWCC 2 cut(s) 242, 529
Eco47III AGCGCT 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 464
EcoICRI GAGCTC 1 cut(s) 464
EcoO109I RGGNCCY 2 cut(s) 242, 529
EcoT14I CCWWGG 2 cut(s) 93, 532
EcoT38I GRGCYC 1 cut(s) 466
ErhI CCWWGG 2 cut(s) 93, 532
FaeI CATG 5 cut(s) 292, 321, 631, 646, 688
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FaqI GGGAC 1 cut(s) 255
FatI CATG 5 cut(s) 288, 317, 627, 642, 684
Fnu4HI GCNGC 1 cut(s) 380
FokI GGATG 1 cut(s) 608
FriOI GRGCYC 1 cut(s) 466
Fsp4HI GCNGC 1 cut(s) 380
FspBI CTAG 2 cut(s) 164, 533
GlaI GCGC 1 cut(s) 305
GluI GCNGC 1 cut(s) 380
HaeII RGCGCY 1 cut(s) 307
HapII CCGG 1 cut(s) 239
HhaI GCGC 1 cut(s) 306
Hin1II CATG 5 cut(s) 292, 321, 631, 646, 688
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HinfI GANTC 4 cut(s) 24, 75, 142, 488
HpaII CCGG 1 cut(s) 239
HphI GGTGA 3 cut(s) 26, 330, 632
Hpy166II GTNNAC 2 cut(s) 230, 544
Hpy188I TCNGA 5 cut(s) 138, 406, 461, 472, 520
Hpy188III TCNNGA 2 cut(s) 527, 587
Hpy8I GTNNAC 2 cut(s) 230, 544
HpyAV CCTTC 6 cut(s) 212, 257, 345, 345, 487, 671
HpyCH4III ACNGT 4 cut(s) 110, 227, 418, 541
HpyCH4IV ACGT 1 cut(s) 149
HpyCH4V TGCA 7 cut(s) 32, 365, 374, 388, 414, 646, 688
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 385, 574
HpyF3I CTNAG 1 cut(s) 509
HpySE526I ACGT 1 cut(s) 149
Hsp92II CATG 5 cut(s) 292, 321, 631, 646, 688
HspAI GCGC 1 cut(s) 304
Kzo9I GATC 3 cut(s) 393, 406, 523
LmnI GCTCC 2 cut(s) 336, 461
LpnPI CCDG 6 cut(s) 56, 252, 512, 518, 572, 621
Lsp1109I GCAGC 1 cut(s) 391
LweI GCATC 1 cut(s) 401
MaeI CTAG 2 cut(s) 164, 533
MaeII ACGT 1 cut(s) 149
MaeIII GTNAC 3 cut(s) 191, 418, 550
MalI GATC 3 cut(s) 395, 408, 525
MboI GATC 3 cut(s) 393, 406, 523
MboII GAAGA 4 cut(s) 391, 451, 635, 668
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 3 cut(s) 33, 213, 310
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 2 cut(s) 109, 439
MroXI GAANNNNTTC 3 cut(s) 234, 267, 355
MseI TTAA 1 cut(s) 54
MspA1I CMGCKG 1 cut(s) 382
MspI CCGG 1 cut(s) 239
MspR9I CCNGG 1 cut(s) 240
MwoI GCNNNNNNNGC 3 cut(s) 371, 385, 574
NciI CCSGG 1 cut(s) 240
NdeII GATC 3 cut(s) 393, 406, 523
NlaIII CATG 5 cut(s) 292, 321, 631, 646, 688
NlaIV GGNNCC 2 cut(s) 243, 538
NmuCI GTSAC 1 cut(s) 191
NspI RCATGY 3 cut(s) 321, 646, 688
PaeI GCATGC 2 cut(s) 646, 688
PdmI GAANNNNTTC 3 cut(s) 234, 267, 355
PfeI GAWTC 3 cut(s) 24, 75, 142
PflMI CCANNNNNTGG 1 cut(s) 70
PfoI TCCNGGA 1 cut(s) 238
PkrI GCNGC 1 cut(s) 381
PleI GAGTC 1 cut(s) 482
PpsI GAGTC 1 cut(s) 482
PpuMI RGGWCCY 2 cut(s) 242, 529
Psp124BI GAGCTC 1 cut(s) 466
Psp5II RGGWCCY 2 cut(s) 242, 529
PspN4I GGNNCC 2 cut(s) 243, 538
PspPI GGNCC 2 cut(s) 242, 529
PspPPI RGGWCCY 2 cut(s) 242, 529
PstNI CAGNNNCTG 1 cut(s) 422
RsaI GTAC 1 cut(s) 545
RsaNI GTAC 1 cut(s) 544
SacI GAGCTC 1 cut(s) 466
SaqAI TTAA 1 cut(s) 54
SatI GCNGC 1 cut(s) 380
Sau3AI GATC 3 cut(s) 393, 406, 523
Sau96I GGNCC 2 cut(s) 242, 529
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 1 cut(s) 240
SduI GDGCHC 1 cut(s) 466
SetI ASST 9 cut(s) 99, 127, 152, 179, 247, 296, 466, 510, 534
SfaNI GCATC 1 cut(s) 401
SfcI CTRYAG 1 cut(s) 172
SinI GGWCC 2 cut(s) 242, 529
SphI GCATGC 2 cut(s) 646, 688
Sse9I AATT 3 cut(s) 33, 213, 310
SsiI CCGC 2 cut(s) 120, 382
SspMI CTAG 2 cut(s) 164, 533
SstI GAGCTC 1 cut(s) 466
StyD4I CCNGG 1 cut(s) 238
StyI CCWWGG 2 cut(s) 93, 532
TaaI ACNGT 4 cut(s) 110, 227, 418, 541
TaiI ACGT 1 cut(s) 152
TaqI TCGA 6 cut(s) 179, 185, 300, 344, 409, 491
TasI AATT 3 cut(s) 33, 213, 310
TatI WGTACW 1 cut(s) 543
TfiI GAWTC 3 cut(s) 24, 75, 142
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TscAI CASTG 1 cut(s) 232
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 1 cut(s) 379
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 1 cut(s) 155
TspGWI ACGGA 1 cut(s) 538
TspRI CASTG 1 cut(s) 232
Van91I CCANNNNNTGG 1 cut(s) 70
VpaK11BI GGWCC 2 cut(s) 242, 529
XapI RAATTY 1 cut(s) 310
XceI RCATGY 3 cut(s) 321, 646, 688
XmaJI CCTAGG 1 cut(s) 532
XmnI GAANNNNTTC 3 cut(s) 234, 267, 355
XspI CTAG 2 cut(s) 164, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.