RLG00000021685

Cell division topological specificity factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016378
Physical Location & Seq
Forward (+)
77261291 .. 77263108
1818 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021685

Sequence Viewer

Length: 789 bp
ATGGTGTTTAGAGGCGGAGCCGCCAAATACACAGTTCACAGTTCACAGTTCACAGTACACTCTTCAGCTGAAAACCCAAACTGGGATAGTTCTGGGGAGAATAAAGCAGCTAGTGAAAGTAAAATGGCGGTTTCTAGGGATTTTTGTATCTCTGCGGCATTGGGTTCTTGTCCTTTTAAACACCACCGTCTGGGAAGCTCTTTCCAACCTTCTAAGGTAACCTTTAAGGGTTGTTATGGTGGAGTATTTAGTAATTCATGGGAGATGAGTCAGATGACACAACCATCCTGCTTAAATTTGAAAGGGTACAAGTCTAAGTGCCACTATAACCAATCATTCGGGATTGCAGAAGACAGTGAACTTATTCCCGAACCTGTAAGCCAAGAAACAGAAGGCTTTCTTCTTGATGCTATCAATATGAGCTTCTTCCAGCGTTTAAACTTGGCATGGAAGATAATGTTCCCTTCACCATCTACTAGGAGAAATTCCAATGCAAAGTTTGCCGAGCAACGCTTGCAGATGATCCTGTTCTCTGATCGATGTGCTGTAACTGATGGTGCAAAGCAGAAGATTGTGAGCAACATTGTGGGTGCTCTCTCTGACTTTGTAGAGATAGATTCACAGGACAAAGTCCAGCTTAGCGTTTCAGCAGATTCAGAAACGGGTACAGTGTACTCTGTTACAGTTCCTGTTCGGCGCGTGAAGCCTGAACATCAAGCTGCGGATGAGCTTGGCGCCATGACAAATGTTCGAGTACAAGGATACAGGGACATCATCAGATTCTGTTGA

Protein Analysis

263

Amino Acids

29.0

Weight (kDa)

8.13

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MinE PF03776 165 - 232 2e-11 Septum formation topological specificity factor MinE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016378)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g47773
malus_domestica MD09G1057200.v1.1
prunus_persica Prupe.3G261900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0166971
rosa_laevigata RLG00000021685
rosa_multiflora Rmu_sc0002735.1_g000025 Rmu_sc0010966.1_g000001 Rmu_sc0025421.1_g000002
rosa_roxburghii Rroxscaffold_2G00083900
rosa_rugosa Rorug02G0527000
rosa_samantha Rh2AG593900 Rh2BG605400 Rh2CG576300 Rh2DG616900
rosa_wichuraiana Rw2G049450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 734
AccB7I CCANNNNNTGG 1 cut(s) 190
AccII CGCG 1 cut(s) 699
AciI CCGC 5 cut(s) 15, 21, 128, 155, 722
AclWI GGATC 1 cut(s) 517
AcsI RAATTY 2 cut(s) 295, 484
AcuI CTGAAG 1 cut(s) 48
AcyI GRCGYC 1 cut(s) 735
AfaI GTAC 5 cut(s) 57, 308, 667, 674, 756
AfiI CCNNNNNNNGG 2 cut(s) 82, 190
AgsI TTSAA 1 cut(s) 301
AluBI AGCT 7 cut(s) 68, 110, 198, 423, 637, 719, 730
AluI AGCT 7 cut(s) 68, 110, 198, 423, 637, 719, 730
Alw21I GWGCWC 1 cut(s) 595
AlwI GGATC 1 cut(s) 517
AlwNI CAGNNNCTG 2 cut(s) 689, 783
ApeKI GCWGC 2 cut(s) 107, 719
ApoI RAATTY 2 cut(s) 295, 484
Asp700I GAANNNNTTC 2 cut(s) 363, 396
AspLEI GCGC 2 cut(s) 699, 737
AsuHPI GGTGA 1 cut(s) 459
BanI GGYRCC 1 cut(s) 734
BbsI GAAGAC 1 cut(s) 357
Bbv12I GWGCWC 1 cut(s) 595
BbvI GCAGC 2 cut(s) 119, 706
BccI CCATC 3 cut(s) 292, 478, 548
BciVI GTATCC 1 cut(s) 755
BfaI CTAG 3 cut(s) 111, 135, 477
BfoI RGCGCY 1 cut(s) 738
BfuI GTATCC 1 cut(s) 755
BisI GCNGC 4 cut(s) 21, 108, 156, 720
BlpI GCTNAGC 1 cut(s) 638
BlsI GCNGC 4 cut(s) 22, 109, 157, 721
BmiI GGNNCC 2 cut(s) 19, 736
BmrI ACTGGG 1 cut(s) 91
BmsI GCATC 1 cut(s) 397
BmuI ACTGGG 1 cut(s) 91
BpiI GAAGAC 1 cut(s) 357
Bpu1102I GCTNAGC 1 cut(s) 638
Bsa29I ATCGAT 1 cut(s) 538
BsaHI GRCGYC 1 cut(s) 735
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 190
Bse1I ACTGG 1 cut(s) 86
BseCI ATCGAT 1 cut(s) 538
BseGI GGATG 2 cut(s) 284, 730
BseLI CCNNNNNNNGG 2 cut(s) 82, 190
BseNI ACTGG 1 cut(s) 86
BseXI GCAGC 2 cut(s) 119, 706
Bsh1236I CGCG 1 cut(s) 699
BshNI GGYRCC 1 cut(s) 734
BshVI ATCGAT 1 cut(s) 538
BsiHKAI GWGCWC 1 cut(s) 595
BslFI GGGAC 1 cut(s) 782
BslI CCNNNNNNNGG 2 cut(s) 82, 190
BsmFI GGGAC 1 cut(s) 782
Bsp1286I GDGCHC 1 cut(s) 595
Bsp143I GATC 2 cut(s) 522, 535
Bsp1720I GCTNAGC 1 cut(s) 638
BspACI CCGC 5 cut(s) 15, 21, 128, 155, 722
BspDI ATCGAT 1 cut(s) 538
BspFNI CGCG 1 cut(s) 699
BspLI GGNNCC 2 cut(s) 19, 736
BspPI GGATC 1 cut(s) 517
BspT107I GGYRCC 1 cut(s) 734
BsrI ACTGG 1 cut(s) 86
BssMI GATC 2 cut(s) 522, 535
BssNI GRCGYC 1 cut(s) 735
Bst4CI ACNGT 8 cut(s) 34, 41, 48, 55, 188, 356, 670, 685
Bst6I CTCTTC 1 cut(s) 67
BstACI GRCGYC 1 cut(s) 735
BstAPI GCANNNNNTGC 2 cut(s) 500, 514
BstC8I GCNNGC 1 cut(s) 515
BstDEI CTNAG 3 cut(s) 213, 315, 638
BstEII GGTNACC 1 cut(s) 217
BstF5I GGATG 2 cut(s) 284, 730
BstFNI CGCG 1 cut(s) 699
BstH2I RGCGCY 1 cut(s) 738
BstHHI GCGC 2 cut(s) 699, 737
BstKTI GATC 2 cut(s) 525, 538
BstMBI GATC 2 cut(s) 522, 535
BstMWI GCNNNNNNNGC 3 cut(s) 500, 514, 703
BstPI GGTNACC 1 cut(s) 217
BstUI CGCG 1 cut(s) 699
BstV1I GCAGC 2 cut(s) 119, 706
BstV2I GAAGAC 1 cut(s) 357
Bsu15I ATCGAT 1 cut(s) 538
BsuI GTATCC 1 cut(s) 755
BsuTUI ATCGAT 1 cut(s) 538
BtsCI GGATG 2 cut(s) 284, 730
BtsIMutI CAGTG 2 cut(s) 361, 675
Cac8I GCNNGC 1 cut(s) 515
CaiI CAGNNNCTG 2 cut(s) 689, 783
CfoI GCGC 2 cut(s) 699, 737
ClaI ATCGAT 1 cut(s) 538
Csp6I GTAC 5 cut(s) 56, 307, 666, 673, 755
CviAII CATG 3 cut(s) 258, 447, 739
CviQI GTAC 5 cut(s) 56, 307, 666, 673, 755
DdeI CTNAG 3 cut(s) 213, 315, 638
DinI GGCGCC 1 cut(s) 736
DpnI GATC 2 cut(s) 524, 537
DpnII GATC 2 cut(s) 522, 535
DraI TTTAAA 2 cut(s) 178, 438
Eam1104I CTCTTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 67
EciI GGCGGA 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 48
Eco91I GGTNACC 1 cut(s) 217
EcoO65I GGTNACC 1 cut(s) 217
EgeI GGCGCC 1 cut(s) 736
EheI GGCGCC 1 cut(s) 736
FaeI CATG 3 cut(s) 261, 450, 742
FaiI YATR 6 cut(s) 237, 259, 327, 419, 448, 740
FalI AAGNNNNNCTT 6 cut(s) 206, 238, 384, 416, 621, 653
FaqI GGGAC 1 cut(s) 782
FatI CATG 3 cut(s) 257, 446, 738
Fnu4HI GCNGC 4 cut(s) 21, 108, 156, 720
FokI GGATG 2 cut(s) 271, 737
Fsp4HI GCNGC 4 cut(s) 21, 108, 156, 720
FspBI CTAG 3 cut(s) 111, 135, 477
GlaI GCGC 2 cut(s) 698, 736
GluI GCNGC 4 cut(s) 21, 108, 156, 720
HaeII RGCGCY 1 cut(s) 738
HhaI GCGC 2 cut(s) 699, 737
Hin1I GRCGYC 1 cut(s) 735
Hin1II CATG 3 cut(s) 261, 450, 742
Hin6I GCGC 2 cut(s) 697, 735
HinP1I GCGC 2 cut(s) 697, 735
HinfI GANTC 4 cut(s) 268, 617, 653, 780
HphI GGTGA 1 cut(s) 459
Hpy166II GTNNAC 6 cut(s) 37, 44, 51, 58, 359, 673
Hpy188I TCNGA 5 cut(s) 273, 535, 601, 658, 779
Hpy188III TCNNGA 3 cut(s) 340, 368, 404
Hpy8I GTNNAC 6 cut(s) 37, 44, 51, 58, 359, 673
HpyAV CCTTC 3 cut(s) 219, 386, 474
HpyCH4III ACNGT 8 cut(s) 34, 41, 48, 55, 188, 356, 670, 685
HpyCH4V TGCA 4 cut(s) 347, 494, 517, 560
HpyF10VI GCNNNNNNNGC 3 cut(s) 500, 514, 703
HpyF3I CTNAG 3 cut(s) 213, 315, 638
Hsp92I GRCGYC 1 cut(s) 735
Hsp92II CATG 3 cut(s) 261, 450, 742
HspAI GCGC 2 cut(s) 697, 735
KasI GGCGCC 1 cut(s) 734
Kzo9I GATC 2 cut(s) 522, 535
LmnI GCTCC 1 cut(s) 17
Lsp1109I GCAGC 2 cut(s) 119, 706
LweI GCATC 1 cut(s) 397
MaeI CTAG 3 cut(s) 111, 135, 477
MaeIII GTNAC 3 cut(s) 217, 547, 679
MalI GATC 2 cut(s) 524, 537
MboI GATC 2 cut(s) 522, 535
MboII GAAGA 6 cut(s) 54, 362, 392, 418, 463, 580
MhlI GDGCHC 1 cut(s) 595
MluCI AATT 3 cut(s) 253, 295, 484
Mly113I GGCGCC 1 cut(s) 735
MlyI GAGTC 1 cut(s) 277
MmeI TCCRAC 1 cut(s) 229
MnlI CCTC 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 363, 396
MseI TTAA 4 cut(s) 177, 225, 293, 437
MspA1I CMGCKG 1 cut(s) 68
MssI GTTTAAAC 1 cut(s) 438
MvnI CGCG 1 cut(s) 699
MwoI GCNNNNNNNGC 3 cut(s) 500, 514, 703
NarI GGCGCC 1 cut(s) 735
NdeII GATC 2 cut(s) 522, 535
NlaIII CATG 3 cut(s) 261, 450, 742
NlaIV GGNNCC 2 cut(s) 19, 736
NmeAIII GCCGAG 1 cut(s) 529
PdmI GAANNNNTTC 2 cut(s) 363, 396
PfeI GAWTC 3 cut(s) 617, 653, 780
PflFI GACNNNGTC 1 cut(s) 629
PflMI CCANNNNNTGG 1 cut(s) 190
PkrI GCNGC 4 cut(s) 22, 109, 157, 721
PleI GAGTC 1 cut(s) 276
PluTI GGCGCC 1 cut(s) 738
PmeI GTTTAAAC 1 cut(s) 438
PpsI GAGTC 1 cut(s) 276
PspEI GGTNACC 1 cut(s) 217
PspN4I GGNNCC 2 cut(s) 19, 736
PstNI CAGNNNCTG 2 cut(s) 689, 783
PsyI GACNNNGTC 1 cut(s) 629
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 5 cut(s) 57, 308, 667, 674, 756
RsaNI GTAC 5 cut(s) 56, 307, 666, 673, 755
SaqAI TTAA 4 cut(s) 177, 225, 293, 437
SatI GCNGC 4 cut(s) 21, 108, 156, 720
Sau3AI GATC 2 cut(s) 522, 535
SchI GAGTC 1 cut(s) 277
SduI GDGCHC 1 cut(s) 595
SfaNI GCATC 1 cut(s) 397
SfoI GGCGCC 1 cut(s) 736
Sse9I AATT 3 cut(s) 253, 295, 484
SsiI CCGC 5 cut(s) 15, 21, 128, 155, 722
SspDI GGCGCC 1 cut(s) 734
SspMI CTAG 3 cut(s) 111, 135, 477
TaaI ACNGT 8 cut(s) 34, 41, 48, 55, 188, 356, 670, 685
TaqI TCGA 2 cut(s) 538, 751
TasI AATT 3 cut(s) 253, 295, 484
TatI WGTACW 3 cut(s) 55, 672, 754
TauI GCSGC 2 cut(s) 23, 158
TfiI GAWTC 3 cut(s) 617, 653, 780
Tru1I TTAA 4 cut(s) 177, 225, 293, 437
Tru9I TTAA 4 cut(s) 177, 225, 293, 437
TscAI CASTG 2 cut(s) 361, 675
TseI GCWGC 2 cut(s) 107, 719
TspDTI ATGAA 1 cut(s) 246
TspRI CASTG 2 cut(s) 361, 675
Tth111I GACNNNGTC 1 cut(s) 629
Van91I CCANNNNNTGG 1 cut(s) 190
XapI RAATTY 2 cut(s) 295, 484
XmnI GAANNNNTTC 2 cut(s) 363, 396
XspI CTAG 3 cut(s) 111, 135, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.